pycom08g11220

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010420
Physical Location & Seq
Reverse (-)
9532692 .. 9534288
1597 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 687 bp
ATGATCCATCAAAGAGGGTTCAGTGTGCTTGCTAATGGACCTCTTGATATGAGGATGGATCCTCAGGCAAGTCTGAAAGCTGAAGACATTTTGAACTCTTGGCCAGATAATGAGGTAGGGCGCATCCTGCGGGACTATGGGGAAGAAAGTAACTGGTACTCTCTTCAAAATAGAATTGTTAGAGCCCGTTCACAGGGTGGATTGCATACCACTGGTGATTTAGTAGATCTTATAAAGAGTGCAACTCCGCTGAGTCGAGAAGGACGGCAAGGTTGGATTAAGACAGCTACAAGGGTGTTTCAAGCTCTGAGGATAGCCGTGAACGATGAACTGAAGACTCCCGAGGATTCTCTTTATTCTTCTTTTGACTGCCTTGCACCTGGTGGCAGGTTGGCAGTCATCTCTTTCCATAGTTTGGAGGACAGAATTGTGAAACAAACATTTCTGGACATCATCAACATCAAAGACAGAGATGGTGACGTTGACATGAATGAAGAAGAACCACGGAAGAAAGATTTGAGAAAGATCATGAGTGACATTGATGAGAACGAGGCATGGATCAAACAAACGGTACAAGGCTTAAGAGGCATAATTCTCACTAAGAGACCAATTACACCGTCTGAAGCGGAAGAGTGCCTGAACAGCCGGGCTAGAAGTGCCAAGCTCAGGGTGCTTCAGAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_5

Protein Analysis

229

Amino Acids

25.89

Weight (kDa)

6.35

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_5 PF01795 4 - 227 1.5e-55 MraW methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 233
Acc36I ACCTGC 1 cut(s) 378
AccB7I CCANNNNNTGG 1 cut(s) 415
AciI CCGC 3 cut(s) 130, 248, 626
AclWI GGATC 3 cut(s) 53, 66, 566
AcoI YGGCCR 1 cut(s) 101
AcuI CTGAAG 4 cut(s) 102, 353, 642, 659
AdeI CACNNNGTG 2 cut(s) 197, 383
AfaI GTAC 2 cut(s) 158, 573
AfiI CCNNNNNNNGG 3 cut(s) 193, 415, 666
AflII CTTAAG 1 cut(s) 580
AgsI TTSAA 3 cut(s) 94, 167, 302
AjnI CCWGG 1 cut(s) 379
AluBI AGCT 5 cut(s) 80, 287, 305, 664, 682
AluI AGCT 5 cut(s) 80, 287, 305, 664, 682
Alw26I GTCTC 1 cut(s) 598
AlwI GGATC 3 cut(s) 53, 66, 566
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 1 cut(s) 101
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 1 cut(s) 38
AsuC2I CCSGG 1 cut(s) 647
AsuHPI GGTGA 2 cut(s) 227, 488
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 1 cut(s) 38
AxyI CCTNAGG 1 cut(s) 63
BalI TGGCCA 1 cut(s) 103
BamHI GGATCC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 187
BbsI GAAGAC 2 cut(s) 90, 341
BccI CCATC 3 cut(s) 15, 49, 467
BceAI ACGGC 2 cut(s) 281, 302
BciT130I CCWGG 1 cut(s) 381
BcnI CCSGG 1 cut(s) 647
BcoDI GTCTC 1 cut(s) 598
BfaI CTAG 1 cut(s) 651
BfrI CTTAAG 1 cut(s) 580
BfuAI ACCTGC 1 cut(s) 378
BglII AGATCT 1 cut(s) 226
Bme1390I CCNGG 2 cut(s) 381, 647
Bme18I GGWCC 1 cut(s) 38
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 38
BmiI GGNNCC 1 cut(s) 60
BmrFI CCNGG 2 cut(s) 381, 647
BmsI GCATC 1 cut(s) 132
BpiI GAAGAC 2 cut(s) 90, 341
BplI GAGNNNNNCTC 2 cut(s) 229, 261
Bpu10I CCTNAGC 1 cut(s) 665
BpuMI CCSGG 1 cut(s) 647
BsaI GGTCTC 1 cut(s) 598
BsaJI CCNNGG 2 cut(s) 342, 503
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 415, 666
Bse1I ACTGG 2 cut(s) 158, 217
Bse21I CCTNAGG 1 cut(s) 63
BseBI CCWGG 1 cut(s) 381
BseDI CCNNGG 2 cut(s) 342, 503
BseGI GGATG 2 cut(s) 60, 123
BseLI CCNNNNNNNGG 3 cut(s) 193, 415, 666
BseMII CTCAG 4 cut(s) 77, 242, 299, 679
BseNI ACTGG 2 cut(s) 158, 217
BshFI GGCC 1 cut(s) 103
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 1 cut(s) 646
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 3 cut(s) 193, 415, 666
BsmAI GTCTC 1 cut(s) 598
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 1 cut(s) 103
Bso31I GGTCTC 1 cut(s) 598
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 187
Bsp143I GATC 5 cut(s) 3, 58, 226, 525, 558
BspACI CCGC 3 cut(s) 130, 248, 626
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 4 cut(s) 76, 243, 300, 678
BspHI TCATGA 1 cut(s) 528
BspLI GGNNCC 1 cut(s) 60
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 3 cut(s) 53, 66, 566
BspTI CTTAAG 1 cut(s) 580
BspTNI GGTCTC 1 cut(s) 598
BsrI ACTGG 2 cut(s) 158, 217
BssECI CCNNGG 2 cut(s) 342, 503
BssMI GATC 5 cut(s) 3, 58, 226, 525, 558
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 2 cut(s) 571, 618
Bst6I CTCTTC 2 cut(s) 168, 624
BstAFI CTTAAG 1 cut(s) 580
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 5 cut(s) 63, 251, 308, 600, 665
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 2 cut(s) 60, 123
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 5 cut(s) 6, 61, 229, 528, 561
BstMAI GTCTC 1 cut(s) 598
BstMBI GATC 5 cut(s) 3, 58, 226, 525, 558
BstMWI GCNNNNNNNGC 6 cut(s) 127, 585, 642, 656, 670, 679
BstNI CCWGG 1 cut(s) 381
BstSCI CCNGG 2 cut(s) 379, 645
BstV2I GAAGAC 2 cut(s) 90, 341
BstX2I RGATCY 2 cut(s) 58, 226
BstYI RGATCY 2 cut(s) 58, 226
Bsu36I CCTNAGG 1 cut(s) 63
BsuRI GGCC 1 cut(s) 103
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 2 cut(s) 60, 123
BtsIMutI CAGTG 2 cut(s) 28, 210
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 30
CciI TCATGA 1 cut(s) 528
CfoI GCGC 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 38
CsiI ACCWGGT 1 cut(s) 379
Csp6I GTAC 2 cut(s) 157, 572
CviAII CATG 3 cut(s) 487, 529, 555
CviQI GTAC 2 cut(s) 157, 572
DdeI CTNAG 5 cut(s) 63, 251, 308, 600, 665
DpnI GATC 5 cut(s) 5, 60, 228, 527, 560
DpnII GATC 5 cut(s) 3, 58, 226, 525, 558
DraIII CACNNNGTG 2 cut(s) 197, 383
EaeI YGGCCR 1 cut(s) 101
Eam1104I CTCTTC 2 cut(s) 168, 624
EarI CTCTTC 2 cut(s) 168, 624
Eco24I GRGCYC 1 cut(s) 187
Eco31I GGTCTC 1 cut(s) 598
Eco47I GGWCC 1 cut(s) 38
Eco57I CTGAAG 4 cut(s) 102, 353, 642, 659
Eco81I CCTNAGG 1 cut(s) 63
Eco88I CYCGRG 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 379
EcoT38I GRGCYC 1 cut(s) 187
FaeI CATG 3 cut(s) 490, 532, 558
FaiI YATR 9 cut(s) 50, 138, 207, 233, 411, 488, 530, 556, 590
FaqI GGGAC 1 cut(s) 146
FatI CATG 3 cut(s) 486, 528, 554
FauI CCCGC 1 cut(s) 123
FokI GGATG 2 cut(s) 67, 110
FriOI GRGCYC 1 cut(s) 187
FspBI CTAG 1 cut(s) 651
GlaI GCGC 1 cut(s) 122
HaeIII GGCC 1 cut(s) 103
HapII CCGG 1 cut(s) 646
HhaI GCGC 1 cut(s) 123
Hin1II CATG 3 cut(s) 490, 532, 558
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HindIII AAGCTT 1 cut(s) 680
HinfI GANTC 3 cut(s) 253, 337, 347
HpaII CCGG 1 cut(s) 646
HphI GGTGA 2 cut(s) 227, 488
Hpy166II GTNNAC 3 cut(s) 191, 322, 484
Hpy188I TCNGA 4 cut(s) 75, 309, 622, 678
Hpy188III TCNNGA 5 cut(s) 44, 257, 341, 446, 529
Hpy8I GTNNAC 3 cut(s) 191, 322, 484
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 2 cut(s) 571, 618
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 3 cut(s) 205, 242, 377
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 585, 642, 656, 670, 679
HpyF3I CTNAG 5 cut(s) 63, 251, 308, 600, 665
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 3 cut(s) 490, 532, 558
HspAI GCGC 1 cut(s) 121
Kzo9I GATC 5 cut(s) 3, 58, 226, 525, 558
LweI GCATC 1 cut(s) 132
MabI ACCWGGT 1 cut(s) 379
MaeI CTAG 1 cut(s) 651
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 3 cut(s) 149, 476, 533
MalI GATC 5 cut(s) 5, 60, 228, 527, 560
MboI GATC 5 cut(s) 3, 58, 226, 525, 558
MboII GAAGA 9 cut(s) 95, 155, 155, 346, 351, 506, 509, 520, 641
MflI RGATCY 2 cut(s) 58, 226
MhlI GDGCHC 1 cut(s) 187
MlsI TGGCCA 1 cut(s) 103
MluCI AATT 4 cut(s) 174, 426, 591, 609
MluNI TGGCCA 1 cut(s) 103
MlyI GAGTC 2 cut(s) 262, 331
MmeI TCCRAC 1 cut(s) 254
Mox20I TGGCCA 1 cut(s) 103
MscI TGGCCA 1 cut(s) 103
MseI TTAA 3 cut(s) 279, 581, 685
Msp20I TGGCCA 1 cut(s) 103
MspA1I CMGCKG 1 cut(s) 250
MspCI CTTAAG 1 cut(s) 580
MspI CCGG 1 cut(s) 646
MspR9I CCNGG 2 cut(s) 381, 647
MvaI CCWGG 1 cut(s) 381
MwoI GCNNNNNNNGC 6 cut(s) 127, 585, 642, 656, 670, 679
NciI CCSGG 1 cut(s) 647
NdeII GATC 5 cut(s) 3, 58, 226, 525, 558
NlaIII CATG 3 cut(s) 490, 532, 558
NlaIV GGNNCC 1 cut(s) 60
NmuCI GTSAC 2 cut(s) 476, 533
PagI TCATGA 1 cut(s) 528
PfeI GAWTC 1 cut(s) 347
PflMI CCANNNNNTGG 1 cut(s) 415
PleI GAGTC 2 cut(s) 261, 331
PpsI GAGTC 2 cut(s) 261, 331
PsiI TTATAA 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 379
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 1 cut(s) 38
PsuI RGATCY 2 cut(s) 58, 226
RsaI GTAC 2 cut(s) 158, 573
RsaNI GTAC 2 cut(s) 157, 572
SaqAI TTAA 3 cut(s) 279, 581, 685
Sau3AI GATC 5 cut(s) 3, 58, 226, 525, 558
Sau96I GGNCC 1 cut(s) 38
SchI GAGTC 2 cut(s) 262, 331
ScrFI CCNGG 2 cut(s) 381, 647
SduI GDGCHC 1 cut(s) 187
SexAI ACCWGGT 1 cut(s) 379
SfaNI GCATC 1 cut(s) 132
SinI GGWCC 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 580
SmoI CTYRAG 1 cut(s) 580
Sse9I AATT 4 cut(s) 174, 426, 591, 609
SsiI CCGC 3 cut(s) 130, 248, 626
SspMI CTAG 1 cut(s) 651
StyD4I CCNGG 2 cut(s) 379, 645
TaaI ACNGT 2 cut(s) 571, 618
TaiI ACGT 1 cut(s) 483
TaqI TCGA 1 cut(s) 256
TasI AATT 4 cut(s) 174, 426, 591, 609
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 3 cut(s) 279, 581, 685
Tru9I TTAA 3 cut(s) 279, 581, 685
TscAI CASTG 2 cut(s) 28, 217
TseFI GTSAC 2 cut(s) 476, 533
Tsp45I GTSAC 2 cut(s) 476, 533
TspDTI ATGAA 3 cut(s) 342, 503, 507
TspGWI ACGGA 1 cut(s) 520
TspRI CASTG 2 cut(s) 28, 217
Van91I CCANNNNNTGG 1 cut(s) 415
Vha464I CTTAAG 1 cut(s) 580
VpaK11BI GGWCC 1 cut(s) 38
XspI CTAG 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.