pycom08g15000

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010420
Physical Location & Seq
Reverse (-)
14456781 .. 14459146
2366 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1065 bp
ATGGAGGTACCGGTACCCCCATTCCGGGCATGCTCGGCGAAGTTCTGGATGTTTTCCCGGCGTCCAAACAACTCCGGTACCTCGTCGACTGCACTGTCGGAGCTCCGTGTTGATTACTGGCAGATAACTGAAGGGCATCCGGTAATGGAAGTGTATGTTGGGCTTGATGTCGATCCTGTGGCGCTTGAGAAGGCTCGAGCTCGGATCAACTCTGTTTTGCATGACCCGAATCCTTCGTCCCTCAAAGCTTACACCATTTTGGAGAATTTCAGATATGTGAAGTCTCTGCTTTGTGATGTGGATGAGACACTAGTTGAAACAGGGATTGATGGGATACGGATGGATTTGGGAATGTCGTCGATGCAAGTAAATGATCCTCAAAGAGGGTTCAGTGTGCTTGTTAATGGACCTCTTGATATGAGGATGGATCCTCAGGCAAGCCTGAAAGCTGAAGACATTTTGAACACTTGGCCAGATAATGAGGTAGGCTGCATCCTGCGAGACTATGGGGAAGAAAGCAACTGGTTCTCTCTTCAAAATAGAATTGTTAGAGCCCGATCACAGGGTGGATTGCATACCACTGGCGATTTTAACAAAGATCGATCTTATAAAAAGTGCAACTCTGCTGAGTCGAGGATGGCAAGGTTGGATGAAAACAGCTACAAGGGTGTTCAAGCTCAAGCTCTGAGGATAGCCGTGAACGATGAACTGAAGACTCTGGAGGATTCTCTTTATGCTTATTTTGACTGCCTTGCACCGGGTGGTAGGTTGGCAGTCGTCTCTTTACACAGTTTGGAGGACAGAATTGTGAAACAAACGTTTCTAGACATCATCAACGTCAAAGACAGAGATGTTGACGTTGACATGATTGAAGAAGCAGCGGGGAAGAAAGATTTGAGAGAGATCATGAGCGACATTGATGAAAACGAAGAATGGATCAAACAAACGGTACAGGGCTTGAGGGGAATAATTCTCACTAAGAGACCAATTACACGATCTGAAACGGAAGAGAGCCTCAACAGCCTAGCTAGAAGTGCCAAGCTTAGGGTGATTCAGAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_5

Protein Analysis

355

Amino Acids

40.01

Weight (kDa)

5.15

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_5 PF01795 51 - 353 8.4e-55 MraW methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
AasI GACNNNNNNGTC 1 cut(s) 94
Acc65I GGTACC 3 cut(s) 7, 13, 77
AccB1I GGYRCC 3 cut(s) 7, 13, 77
AccI GTMKAC 1 cut(s) 86
AciI CCGC 1 cut(s) 881
AclI AACGTT 1 cut(s) 818
AclWI GGATC 6 cut(s) 167, 212, 368, 422, 435, 944
AcoI YGGCCR 1 cut(s) 470
AcsI RAATTY 1 cut(s) 265
AcuI CTGAAG 3 cut(s) 150, 471, 731
AcyI GRCGYC 1 cut(s) 61
AdeI CACNNNGTG 2 cut(s) 566, 761
AfaI GTAC 4 cut(s) 9, 15, 79, 951
AfiI CCNNNNNNNGG 6 cut(s) 24, 25, 383, 562, 757, 1044
AgeI ACCGGT 1 cut(s) 10
AgsI TTSAA 5 cut(s) 317, 463, 536, 674, 872
AhlI ACTAGT 1 cut(s) 310
AjuI GAANNNNNNNTTGG 2 cut(s) 141, 173
Alw21I GWGCWC 2 cut(s) 105, 202
Alw26I GTCTC 5 cut(s) 288, 299, 495, 784, 976
AlwI GGATC 6 cut(s) 167, 212, 368, 422, 435, 944
Ama87I CYCGRG 1 cut(s) 195
AoxI GGCC 1 cut(s) 470
ApeKI GCWGC 2 cut(s) 489, 878
ApoI RAATTY 1 cut(s) 265
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
AsiGI ACCGGT 1 cut(s) 10
Asp718I GGTACC 3 cut(s) 7, 13, 77
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 1 cut(s) 407
AsuC2I CCSGG 3 cut(s) 26, 58, 759
AsuHPI GGTGA 1 cut(s) 1060
AvaI CYCGRG 1 cut(s) 195
AvaII GGWCC 1 cut(s) 407
AxyI CCTNAGG 1 cut(s) 432
BaeI ACNNNNGTAYC 2 cut(s) 61, 94
BalI TGGCCA 1 cut(s) 472
BamHI GGATCC 1 cut(s) 427
BanI GGYRCC 3 cut(s) 7, 13, 77
BanII GRGCYC 3 cut(s) 105, 202, 556
BbsI GAAGAC 2 cut(s) 459, 719
Bbv12I GWGCWC 2 cut(s) 105, 202
BbvI GCAGC 2 cut(s) 476, 890
BccI CCATC 4 cut(s) 323, 334, 418, 631
BceAI ACGGC 1 cut(s) 680
BciVI GTATCC 1 cut(s) 327
BcnI CCSGG 3 cut(s) 26, 58, 759
BcoDI GTCTC 5 cut(s) 288, 299, 495, 784, 976
BcuI ACTAGT 1 cut(s) 310
BfaI CTAG 4 cut(s) 311, 824, 1025, 1029
BfoI RGCGCY 1 cut(s) 185
BfuI GTATCC 1 cut(s) 327
BisI GCNGC 2 cut(s) 490, 879
BlsI GCNGC 2 cut(s) 491, 880
Bme1390I CCNGG 3 cut(s) 26, 58, 759
Bme18I GGWCC 1 cut(s) 407
BmeT110I CYCGRG 1 cut(s) 195
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 4 cut(s) 9, 15, 79, 429
BmrFI CCNGG 3 cut(s) 26, 58, 759
BmsI GCATC 3 cut(s) 145, 351, 501
BpiI GAAGAC 2 cut(s) 459, 719
BpmI CTGGAG 1 cut(s) 740
Bpu10I CCTNAGC 1 cut(s) 1043
BpuEI CTTGAG 3 cut(s) 206, 663, 979
BpuMI CCSGG 3 cut(s) 26, 58, 759
Bsa29I ATCGAT 1 cut(s) 601
BsaBI GATNNNNATC 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 61
BsaI GGTCTC 1 cut(s) 976
BsaWI WCCGGW 3 cut(s) 10, 74, 139
Bsc4I CCNNNNNNNGG 6 cut(s) 24, 25, 383, 562, 757, 1044
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 3 cut(s) 122, 527, 586
Bse21I CCTNAGG 1 cut(s) 432
Bse8I GATNNNNATC 1 cut(s) 171
BseCI ATCGAT 1 cut(s) 601
BseGI GGATG 8 cut(s) 54, 136, 307, 345, 429, 492, 642, 655
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 6 cut(s) 24, 25, 383, 562, 757, 1044
BseMII CTCAG 3 cut(s) 446, 618, 677
BseNI ACTGG 3 cut(s) 122, 527, 586
BseXI GCAGC 2 cut(s) 476, 890
BsgI GTGCAG 1 cut(s) 75
BshFI GGCC 1 cut(s) 472
BshNI GGYRCC 3 cut(s) 7, 13, 77
BshTI ACCGGT 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 601
BsiHKAI GWGCWC 2 cut(s) 105, 202
BsiHKCI CYCGRG 1 cut(s) 195
BsiSI CCGG 6 cut(s) 11, 25, 58, 75, 140, 758
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 6 cut(s) 24, 25, 383, 562, 757, 1044
BsmAI GTCTC 5 cut(s) 288, 299, 495, 784, 976
BsmBI CGTCTC 1 cut(s) 784
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 1 cut(s) 472
Bso31I GGTCTC 1 cut(s) 976
BsoBI CYCGRG 1 cut(s) 195
Bsp1286I GDGCHC 3 cut(s) 105, 202, 556
BspACI CCGC 1 cut(s) 881
BspANI GGCC 1 cut(s) 472
BspCNI CTCAG 3 cut(s) 445, 619, 678
BspDI ATCGAT 1 cut(s) 601
BspHI TCATGA 1 cut(s) 906
BspLI GGNNCC 4 cut(s) 9, 15, 79, 429
BspPI GGATC 6 cut(s) 167, 212, 368, 422, 435, 944
BspT107I GGYRCC 3 cut(s) 7, 13, 77
BspTNI GGTCTC 1 cut(s) 976
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 3 cut(s) 122, 527, 586
BssAI RCCGGY 1 cut(s) 10
BssNI GRCGYC 1 cut(s) 61
Bst4CI ACNGT 3 cut(s) 96, 791, 949
Bst6I CTCTTC 2 cut(s) 537, 1002
BstACI GRCGYC 1 cut(s) 61
BstC8I GCNNGC 2 cut(s) 31, 439
BstDEI CTNAG 5 cut(s) 432, 627, 686, 978, 1043
BstENI CCTNNNNNAGG 1 cut(s) 381
BstF5I GGATG 8 cut(s) 54, 136, 307, 345, 429, 492, 642, 655
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 1 cut(s) 184
BstMAI GTCTC 5 cut(s) 288, 299, 495, 784, 976
BstMWI GCNNNNNNNGC 3 cut(s) 35, 1020, 1034
BstNSI RCATGY 1 cut(s) 33
BstSCI CCNGG 3 cut(s) 24, 56, 757
BstV1I GCAGC 2 cut(s) 476, 890
BstV2I GAAGAC 2 cut(s) 459, 719
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
Bsu15I ATCGAT 1 cut(s) 601
Bsu36I CCTNAGG 1 cut(s) 432
BsuI GTATCC 1 cut(s) 327
BsuRI GGCC 1 cut(s) 472
BsuTUI ATCGAT 1 cut(s) 601
BtsCI GGATG 8 cut(s) 54, 136, 307, 345, 429, 492, 642, 655
BtsIMutI CAGTG 3 cut(s) 92, 397, 579
Cac8I GCNNGC 2 cut(s) 31, 439
CciI TCATGA 1 cut(s) 906
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 407
ClaI ATCGAT 1 cut(s) 601
CseI GACGC 1 cut(s) 50
Csp6I GTAC 4 cut(s) 8, 14, 78, 950
CspAI ACCGGT 1 cut(s) 10
CviAII CATG 4 cut(s) 30, 221, 865, 907
CviQI GTAC 4 cut(s) 8, 14, 78, 950
DdeI CTNAG 5 cut(s) 432, 627, 686, 978, 1043
DraIII CACNNNGTG 2 cut(s) 566, 761
DrdI GACNNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 470
Eam1104I CTCTTC 2 cut(s) 537, 1002
EarI CTCTTC 2 cut(s) 537, 1002
Ecl136II GAGCTC 2 cut(s) 103, 200
Eco24I GRGCYC 3 cut(s) 105, 202, 556
Eco31I GGTCTC 1 cut(s) 976
Eco47I GGWCC 1 cut(s) 407
Eco53kI GAGCTC 2 cut(s) 103, 200
Eco57I CTGAAG 3 cut(s) 150, 471, 731
Eco81I CCTNAGG 1 cut(s) 432
Eco88I CYCGRG 1 cut(s) 195
EcoICRI GAGCTC 2 cut(s) 103, 200
EcoNI CCTNNNNNAGG 1 cut(s) 381
EcoT38I GRGCYC 3 cut(s) 105, 202, 556
Esp3I CGTCTC 1 cut(s) 784
FaeI CATG 4 cut(s) 33, 224, 868, 910
FaqI GGGAC 1 cut(s) 223
FatI CATG 4 cut(s) 29, 220, 864, 906
FauI CCCGC 1 cut(s) 874
FblI GTMKAC 1 cut(s) 86
Fnu4HI GCNGC 2 cut(s) 490, 879
FokI GGATG 8 cut(s) 61, 123, 314, 352, 436, 479, 649, 662
FriOI GRGCYC 3 cut(s) 105, 202, 556
Fsp4HI GCNGC 2 cut(s) 490, 879
FspBI CTAG 4 cut(s) 311, 824, 1025, 1029
GlaI GCGC 1 cut(s) 183
GluI GCNGC 2 cut(s) 490, 879
GsuI CTGGAG 1 cut(s) 740
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 1 cut(s) 472
HapII CCGG 6 cut(s) 11, 25, 58, 75, 140, 758
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 184
Hin1I GRCGYC 1 cut(s) 61
Hin1II CATG 4 cut(s) 33, 224, 868, 910
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HincII GTYRAC 3 cut(s) 87, 856, 862
HindII GTYRAC 3 cut(s) 87, 856, 862
HindIII AAGCTT 3 cut(s) 246, 1040, 1058
HinfI GANTC 5 cut(s) 229, 629, 715, 725, 1051
HpaII CCGG 6 cut(s) 11, 25, 58, 75, 140, 758
HphI GGTGA 1 cut(s) 1060
Hpy166II GTNNAC 4 cut(s) 87, 700, 856, 862
Hpy188I TCNGA 6 cut(s) 100, 204, 272, 687, 1000, 1056
Hpy188III TCNNGA 5 cut(s) 46, 413, 719, 824, 907
Hpy8I GTNNAC 4 cut(s) 87, 700, 856, 862
Hpy99I CGWCG 2 cut(s) 88, 361
HpyAV CCTTC 3 cut(s) 125, 184, 243
HpyCH4III ACNGT 3 cut(s) 96, 791, 949
HpyCH4IV ACGT 3 cut(s) 818, 837, 858
HpyCH4V TGCA 7 cut(s) 92, 220, 364, 492, 574, 618, 755
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 1020, 1034
HpyF3I CTNAG 5 cut(s) 432, 627, 686, 978, 1043
HpySE526I ACGT 3 cut(s) 818, 837, 858
Hsp92I GRCGYC 1 cut(s) 61
Hsp92II CATG 4 cut(s) 33, 224, 868, 910
HspAI GCGC 1 cut(s) 182
KpnI GGTACC 3 cut(s) 11, 17, 81
LmnI GCTCC 2 cut(s) 100, 108
Lsp1109I GCAGC 2 cut(s) 476, 890
LweI GCATC 3 cut(s) 145, 351, 501
MaeI CTAG 4 cut(s) 311, 824, 1025, 1029
MaeII ACGT 3 cut(s) 818, 837, 858
MboII GAAGA 8 cut(s) 464, 524, 524, 724, 884, 898, 941, 1019
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 3 cut(s) 105, 202, 556
MlsI TGGCCA 1 cut(s) 472
MluCI AATT 5 cut(s) 265, 543, 804, 969, 987
MluNI TGGCCA 1 cut(s) 472
MlyI GAGTC 2 cut(s) 638, 709
MmeI TCCRAC 2 cut(s) 78, 627
Mox20I TGGCCA 1 cut(s) 472
MscI TGGCCA 1 cut(s) 472
MseI TTAA 3 cut(s) 402, 591, 1063
Msp20I TGGCCA 1 cut(s) 472
MspA1I CMGCKG 1 cut(s) 881
MspI CCGG 6 cut(s) 11, 25, 58, 75, 140, 758
MspR9I CCNGG 3 cut(s) 26, 58, 759
MwoI GCNNNNNNNGC 3 cut(s) 35, 1020, 1034
NciI CCSGG 3 cut(s) 26, 58, 759
NlaIII CATG 4 cut(s) 33, 224, 868, 910
NlaIV GGNNCC 4 cut(s) 9, 15, 79, 429
NmeAIII GCCGAG 1 cut(s) 14
NspI RCATGY 1 cut(s) 33
PaeI GCATGC 1 cut(s) 33
PaeR7I CTCGAG 1 cut(s) 195
PagI TCATGA 1 cut(s) 906
PfeI GAWTC 3 cut(s) 229, 725, 1051
PinAI ACCGGT 1 cut(s) 10
PkrI GCNGC 2 cut(s) 491, 880
PleI GAGTC 2 cut(s) 637, 709
PpsI GAGTC 2 cut(s) 637, 709
PsiI TTATAA 1 cut(s) 609
Psp124BI GAGCTC 2 cut(s) 105, 202
Psp1406I AACGTT 1 cut(s) 818
PspN4I GGNNCC 4 cut(s) 9, 15, 79, 429
PspPI GGNCC 1 cut(s) 407
PspXI VCTCGAGB 1 cut(s) 195
PsuI RGATCY 1 cut(s) 427
RsaI GTAC 4 cut(s) 9, 15, 79, 951
RsaNI GTAC 4 cut(s) 8, 14, 78, 950
SacI GAGCTC 2 cut(s) 105, 202
SalI GTCGAC 1 cut(s) 85
SaqAI TTAA 3 cut(s) 402, 591, 1063
SatI GCNGC 2 cut(s) 490, 879
Sau96I GGNCC 1 cut(s) 407
SchI GAGTC 2 cut(s) 638, 709
ScrFI CCNGG 3 cut(s) 26, 58, 759
SduI GDGCHC 3 cut(s) 105, 202, 556
SfaNI GCATC 3 cut(s) 145, 351, 501
Sfr274I CTCGAG 1 cut(s) 195
SinI GGWCC 1 cut(s) 407
SlaI CTCGAG 1 cut(s) 195
SmlI CTYRAG 4 cut(s) 185, 195, 678, 958
SmoI CTYRAG 4 cut(s) 185, 195, 678, 958
SpeI ACTAGT 1 cut(s) 310
SphI GCATGC 1 cut(s) 33
Sse9I AATT 5 cut(s) 265, 543, 804, 969, 987
SsiI CCGC 1 cut(s) 881
SspMI CTAG 4 cut(s) 311, 824, 1025, 1029
SstI GAGCTC 2 cut(s) 105, 202
StyD4I CCNGG 3 cut(s) 24, 56, 757
TaaI ACNGT 3 cut(s) 96, 791, 949
TaiI ACGT 3 cut(s) 821, 840, 861
TaqI TCGA 6 cut(s) 86, 171, 196, 359, 601, 632
TasI AATT 5 cut(s) 265, 543, 804, 969, 987
TfiI GAWTC 3 cut(s) 229, 725, 1051
Tru1I TTAA 3 cut(s) 402, 591, 1063
Tru9I TTAA 3 cut(s) 402, 591, 1063
TscAI CASTG 3 cut(s) 99, 397, 586
TseI GCWGC 2 cut(s) 489, 878
TspDTI ATGAA 3 cut(s) 666, 720, 936
TspGWI ACGGA 3 cut(s) 95, 352, 1019
TspRI CASTG 3 cut(s) 99, 397, 586
VpaK11BI GGWCC 1 cut(s) 407
XagI CCTNNNNNAGG 1 cut(s) 381
XapI RAATTY 1 cut(s) 265
XbaI TCTAGA 1 cut(s) 823
XceI RCATGY 1 cut(s) 33
XhoI CTCGAG 1 cut(s) 195
XmiI GTMKAC 1 cut(s) 86
XspI CTAG 4 cut(s) 311, 824, 1025, 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.