pycom08g16230

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004542
Physical Location & Seq
Reverse (-)
16101002 .. 16107787
6786 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1080 bp
ATGGCGCTTCGTTTCGAGGTTTTGGGGAGGTTTAACCGTGCTCGTGCAGCTAGATTGACCCTGCCTCACTTTGTGTGCCAGACACCACTTTTCATGCCCGTTGGAACGCAAGGAACTATAAAAGGGTTGACAACCGAACAGCTTGAAGATATTGGTGCTCAAATAATACTTGGCAATACGTATCATTTGGCACTTCGTCCGACCTCTGAGCTTCTTGATGAGTTGGGAGGTCTCCACAAATTTATGAACTGGCCAAGAGCATTGCTTACTGATTCTGGTGGCTTTCAGATGGTATCATTATTGCATTTAGCTGACATCACCGAAGAAGGTGTAACATTTCAGTCACCAGTTGATGGAAAGCCAATGCTTTTGACACCTGAGGAGTCAATTCAAATCCAAAACAGAATTGGAGCAGATATTATTATGGCCCTCGATGATGTAGTCAAAACCACCACCACTGGTCCAAGAGTTAAGGAGGCCATGGATCGCACTATACGATGGATAGACAGATGCATTAAAGCTCACAAGAGACCAACAGAGCAGAACTTATTTGGTATTGTACAAGGTGGCTTAGATCCTGTATTAAGGTATGCTATTGGCGGTCTTTCTGGCGGTGAAAGTAAGGATTCATTTTGGCGTGTTGTTGCACAGTGCACTGCCGCATTGCCTGAGGAGAAACCGCGATATGTTATGGGTGTTGGTTATCCTCTGGATATTGTAGTTTGCAGTGCTCTAGGTGCTGACATGTATGACTGTGTTTATCCCACACGTACTGCTCGCTTTGGTACAGCTCTTGTACGCGAGGGAGTGCTGAAACTCAAACAGAAAGCGATGGCTGAAGATACCCGCCCCGTTGATCCTACATGTGATTGTATGGTCTGCAAAAATTATACTAGAGCTTACATTCATTGCCTTGTTACGAAAGATGCTATGGGATCTCAGCTTCTGTCATATCACAATTTATATTACATGATGAAGTTTCCGAAAGGTGATGTCCCCGAGTGGGTCTGTGATGCCATGGAGGTTGCTGGAATCGACATTTCTTCGTGCTGCACTCCATTTTCATCATCCCAAGATTAG

Protein Analysis

360

Amino Acids

39.75

Weight (kDa)

6.02

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 13 - 327 3.6e-137 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 353
AccII CGCG 2 cut(s) 682, 801
AciI CCGC 5 cut(s) 600, 612, 660, 680, 847
AclWI GGATC 4 cut(s) 492, 569, 851, 943
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 858
AdeI CACNNNGTG 1 cut(s) 73
AfaI GTAC 4 cut(s) 561, 772, 787, 798
AfiI CCNNNNNNNGG 2 cut(s) 353, 1003
AflIII ACRYGT 3 cut(s) 744, 767, 863
AgsI TTSAA 2 cut(s) 146, 392
AluBI AGCT 8 cut(s) 50, 142, 211, 311, 521, 791, 899, 943
AluI AGCT 8 cut(s) 50, 142, 211, 311, 521, 791, 899, 943
Alw21I GWGCWC 4 cut(s) 43, 160, 656, 733
Alw26I GTCTC 2 cut(s) 236, 523
Alw44I GTGCAC 1 cut(s) 652
AlwI GGATC 4 cut(s) 492, 569, 851, 943
AlwNI CAGNNNCTG 1 cut(s) 946
Ama87I CYCGRG 1 cut(s) 998
AoxI GGCC 3 cut(s) 251, 426, 477
ApaLI GTGCAC 1 cut(s) 652
ApeKI GCWGC 2 cut(s) 47, 1050
ApoI RAATTY 1 cut(s) 239
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 427, 461
AsuHPI GGTGA 4 cut(s) 310, 336, 626, 1001
AvaI CYCGRG 1 cut(s) 998
AvaII GGWCC 1 cut(s) 461
AxyI CCTNAGG 2 cut(s) 378, 669
BaeGI GKGCMC 1 cut(s) 656
BalI TGGCCA 1 cut(s) 253
BauI CACGAG 1 cut(s) 42
Bbv12I GWGCWC 4 cut(s) 43, 160, 656, 733
BbvI GCAGC 2 cut(s) 59, 1037
BccI CCATC 4 cut(s) 283, 347, 492, 826
BcoDI GTCTC 2 cut(s) 236, 523
BfaI CTAG 3 cut(s) 51, 734, 894
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 3 cut(s) 48, 660, 1051
BlsI GCNGC 3 cut(s) 49, 661, 1052
Bme18I GGWCC 1 cut(s) 461
BmeT110I CYCGRG 1 cut(s) 998
BmgT120I GGNCC 2 cut(s) 427, 461
BmsI GCATC 3 cut(s) 500, 916, 1003
BsaAI YACGTR 2 cut(s) 180, 770
BsaI GGTCTC 2 cut(s) 236, 523
BsaJI CCNNGG 2 cut(s) 480, 1017
Bsc4I CCNNNNNNNGG 2 cut(s) 353, 1003
Bse1I ACTGG 3 cut(s) 254, 347, 463
Bse21I CCTNAGG 2 cut(s) 378, 669
Bse3DI GCAATG 3 cut(s) 260, 662, 907
BseDI CCNNGG 2 cut(s) 480, 1017
BseGI GGATG 1 cut(s) 1067
BseLI CCNNNNNNNGG 2 cut(s) 353, 1003
BseMI GCAATG 3 cut(s) 260, 662, 907
BseMII CTCAG 4 cut(s) 198, 369, 660, 953
BseNI ACTGG 3 cut(s) 254, 347, 463
BseRI GAGGAG 2 cut(s) 395, 686
BseSI GKGCMC 1 cut(s) 656
BseXI GCAGC 2 cut(s) 59, 1037
BsgI GTGCAG 2 cut(s) 66, 1036
Bsh1236I CGCG 2 cut(s) 682, 801
BshFI GGCC 3 cut(s) 253, 428, 479
BsiHKAI GWGCWC 4 cut(s) 43, 160, 656, 733
BsiHKCI CYCGRG 1 cut(s) 998
BslFI GGGAC 1 cut(s) 980
BslI CCNNNNNNNGG 2 cut(s) 353, 1003
BsmAI GTCTC 2 cut(s) 236, 523
BsmFI GGGAC 1 cut(s) 980
BsnI GGCC 3 cut(s) 253, 428, 479
Bso31I GGTCTC 2 cut(s) 236, 523
BsoBI CYCGRG 1 cut(s) 998
Bsp1286I GDGCHC 4 cut(s) 43, 160, 656, 733
Bsp1407I TGTACA 1 cut(s) 559
Bsp143I GATC 4 cut(s) 484, 574, 856, 935
Bsp19I CCATGG 2 cut(s) 480, 1017
BspACI CCGC 5 cut(s) 600, 612, 660, 680, 847
BspANI GGCC 3 cut(s) 253, 428, 479
BspCNI CTCAG 4 cut(s) 199, 370, 661, 952
BspFNI CGCG 2 cut(s) 682, 801
BspPI GGATC 4 cut(s) 492, 569, 851, 943
BspTNI GGTCTC 2 cut(s) 236, 523
BsrDI GCAATG 3 cut(s) 260, 662, 907
BsrGI TGTACA 1 cut(s) 559
BsrI ACTGG 3 cut(s) 254, 347, 463
BssECI CCNNGG 2 cut(s) 480, 1017
BssMI GATC 4 cut(s) 484, 574, 856, 935
BssSI CACGAG 1 cut(s) 42
BssT1I CCWWGG 2 cut(s) 480, 1017
Bst2BI CACGAG 1 cut(s) 42
Bst4CI ACNGT 3 cut(s) 38, 651, 755
BstAUI TGTACA 1 cut(s) 559
BstBAI YACGTR 2 cut(s) 180, 770
BstC8I GCNNGC 1 cut(s) 778
BstDEI CTNAG 5 cut(s) 207, 378, 571, 669, 939
BstDSI CCRYGG 2 cut(s) 480, 1017
BstF5I GGATG 1 cut(s) 1067
BstFNI CGCG 2 cut(s) 682, 801
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 4 cut(s) 487, 577, 859, 938
BstMAI GTCTC 2 cut(s) 236, 523
BstMBI GATC 4 cut(s) 484, 574, 856, 935
BstMWI GCNNNNNNNGC 2 cut(s) 47, 737
BstNSI RCATGY 2 cut(s) 748, 867
BstSLI GKGCMC 1 cut(s) 656
BstSNI TACGTA 1 cut(s) 180
BstUI CGCG 2 cut(s) 682, 801
BstV1I GCAGC 2 cut(s) 59, 1037
BstX2I RGATCY 2 cut(s) 574, 935
BstYI RGATCY 2 cut(s) 574, 935
Bsu36I CCTNAGG 2 cut(s) 378, 669
BsuRI GGCC 3 cut(s) 253, 428, 479
BtgI CCRYGG 2 cut(s) 480, 1017
BtgZI GCGATG 1 cut(s) 845
BtsCI GGATG 1 cut(s) 1067
BtsI GCAGTG 2 cut(s) 654, 733
BtsIMutI CAGTG 4 cut(s) 456, 654, 656, 733
Cac8I GCNNGC 1 cut(s) 778
CaiI CAGNNNCTG 1 cut(s) 946
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 427, 461
Csp6I GTAC 4 cut(s) 560, 771, 786, 797
CviAII CATG 6 cut(s) 94, 481, 745, 864, 970, 1018
CviQI GTAC 4 cut(s) 560, 771, 786, 797
DdeI CTNAG 5 cut(s) 207, 378, 571, 669, 939
DpnI GATC 4 cut(s) 486, 576, 858, 937
DpnII GATC 4 cut(s) 484, 574, 856, 935
DraIII CACNNNGTG 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 251
Eco105I TACGTA 1 cut(s) 180
Eco130I CCWWGG 2 cut(s) 480, 1017
Eco31I GGTCTC 2 cut(s) 236, 523
Eco47I GGWCC 1 cut(s) 461
Eco57I CTGAAG 1 cut(s) 858
Eco81I CCTNAGG 2 cut(s) 378, 669
Eco88I CYCGRG 1 cut(s) 998
EcoT14I CCWWGG 2 cut(s) 480, 1017
EcoT22I ATGCAT 1 cut(s) 515
ErhI CCWWGG 2 cut(s) 480, 1017
FaeI CATG 6 cut(s) 97, 484, 748, 867, 973, 1021
FaqI GGGAC 1 cut(s) 980
FatI CATG 6 cut(s) 93, 480, 744, 863, 969, 1017
FauI CCCGC 1 cut(s) 854
Fnu4HI GCNGC 3 cut(s) 48, 660, 1051
FokI GGATG 1 cut(s) 1054
Fsp4HI GCNGC 3 cut(s) 48, 660, 1051
FspBI CTAG 3 cut(s) 51, 734, 894
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 48, 660, 1051
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 253, 428, 479
HhaI GCGC 1 cut(s) 7
Hin1II CATG 6 cut(s) 97, 484, 748, 867, 973, 1021
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HinfI GANTC 4 cut(s) 272, 383, 626, 1032
HphI GGTGA 4 cut(s) 310, 336, 626, 1001
Hpy166II GTNNAC 2 cut(s) 129, 654
Hpy188I TCNGA 4 cut(s) 201, 208, 288, 984
Hpy188III TCNNGA 2 cut(s) 215, 710
Hpy8I GTNNAC 2 cut(s) 129, 654
HpyAV CCTTC 1 cut(s) 320
HpyCH4III ACNGT 3 cut(s) 38, 651, 755
HpyCH4IV ACGT 2 cut(s) 179, 769
HpyCH4V TGCA 8 cut(s) 47, 304, 513, 647, 654, 726, 882, 1053
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 737
HpyF3I CTNAG 5 cut(s) 207, 378, 571, 669, 939
HpySE526I ACGT 2 cut(s) 179, 769
Hsp92II CATG 6 cut(s) 97, 484, 748, 867, 973, 1021
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 4 cut(s) 484, 574, 856, 935
LmnI GCTCC 1 cut(s) 410
Lsp1109I GCAGC 2 cut(s) 59, 1037
LweI GCATC 3 cut(s) 500, 916, 1003
MaeI CTAG 3 cut(s) 51, 734, 894
MaeII ACGT 2 cut(s) 179, 769
MaeIII GTNAC 3 cut(s) 331, 342, 916
MalI GATC 4 cut(s) 486, 576, 858, 937
MboI GATC 4 cut(s) 484, 574, 856, 935
MboII GAAGA 4 cut(s) 158, 335, 851, 1035
MflI RGATCY 2 cut(s) 574, 935
MhlI GDGCHC 4 cut(s) 43, 160, 656, 733
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 5 cut(s) 239, 387, 405, 886, 958
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 1 cut(s) 392
MmeI TCCRAC 2 cut(s) 82, 224
Mox20I TGGCCA 1 cut(s) 253
Mph1103I ATGCAT 1 cut(s) 515
MscI TGGCCA 1 cut(s) 253
MseI TTAA 4 cut(s) 33, 471, 516, 584
Msp20I TGGCCA 1 cut(s) 253
MvnI CGCG 2 cut(s) 682, 801
MwoI GCNNNNNNNGC 2 cut(s) 47, 737
NcoI CCATGG 2 cut(s) 480, 1017
NdeII GATC 4 cut(s) 484, 574, 856, 935
NlaIII CATG 6 cut(s) 97, 484, 748, 867, 973, 1021
NmuCI GTSAC 1 cut(s) 342
NsiI ATGCAT 1 cut(s) 515
NspI RCATGY 2 cut(s) 748, 867
PciI ACATGT 2 cut(s) 744, 863
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PfeI GAWTC 3 cut(s) 272, 626, 1032
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 3 cut(s) 49, 661, 1052
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Ppu21I YACGTR 2 cut(s) 180, 770
PscI ACATGT 2 cut(s) 744, 863
PspPI GGNCC 2 cut(s) 427, 461
PstNI CAGNNNCTG 1 cut(s) 946
PsuI RGATCY 2 cut(s) 574, 935
RsaI GTAC 4 cut(s) 561, 772, 787, 798
RsaNI GTAC 4 cut(s) 560, 771, 786, 797
SaqAI TTAA 4 cut(s) 33, 471, 516, 584
SatI GCNGC 3 cut(s) 48, 660, 1051
Sau3AI GATC 4 cut(s) 484, 574, 856, 935
Sau96I GGNCC 2 cut(s) 427, 461
SchI GAGTC 1 cut(s) 392
SduI GDGCHC 4 cut(s) 43, 160, 656, 733
SfaNI GCATC 3 cut(s) 500, 916, 1003
SinI GGWCC 1 cut(s) 461
SnaBI TACGTA 1 cut(s) 180
Sse9I AATT 5 cut(s) 239, 387, 405, 886, 958
SsiI CCGC 5 cut(s) 600, 612, 660, 680, 847
SspMI CTAG 3 cut(s) 51, 734, 894
StyI CCWWGG 2 cut(s) 480, 1017
TaaI ACNGT 3 cut(s) 38, 651, 755
TaiI ACGT 2 cut(s) 182, 772
TaqI TCGA 3 cut(s) 15, 432, 1035
TasI AATT 5 cut(s) 239, 387, 405, 886, 958
TatI WGTACW 1 cut(s) 559
TauI GCSGC 1 cut(s) 662
TfiI GAWTC 3 cut(s) 272, 626, 1032
Tru1I TTAA 4 cut(s) 33, 471, 516, 584
Tru9I TTAA 4 cut(s) 33, 471, 516, 584
TscAI CASTG 4 cut(s) 463, 656, 661, 733
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 2 cut(s) 47, 1050
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 6 cut(s) 82, 260, 618, 896, 989, 1053
TspRI CASTG 4 cut(s) 463, 656, 661, 733
Van91I CCANNNNNTGG 1 cut(s) 353
VneI GTGCAC 1 cut(s) 652
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 1 cut(s) 239
XceI RCATGY 2 cut(s) 748, 867
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XspI CTAG 3 cut(s) 51, 734, 894
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.