pycom08g19620

Transmembrane protein 87B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011281
Physical Location & Seq
Forward (+)
19623343 .. 19625513
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1044 bp
ATGGATTATCAAGAGATTTTAATCCCCAAAACTGGGATGTATAACTTGTTTTTTGTAGCATGTGATCCAAAACTAAAGGGACTAACTATGAGCGGGAAAACTTCGTGGAAAAATCCCAATGGTTATCTACCGGGTCGAATGGCTCCATTAATGAAGTTTTATGTGTTTATCGCACTTGCTTACGTGTTGCTTGGAATAATTTGGTTCACTCAGTATGTGAGGTTTTGGAAGGATATTCTGCAACTTCAGCACTGCATCACAGCTGTTATCGCTCTTGGGTTGTTTGAGATGATTCTTTGGTATTTTGATTACGTAAATTTTAATAATACAGGGATGAGGCCAATTGTTGTTACAACATTGGTTGTGACAGTTGGAGCTGTGAGAAAAACAGTTTCCCGTCTTCTTATCCTCATTGTTTCAATGGGTTATGGTGTGGTGCGGCCCACTCTCGGGGGCCTCACCTCAAAGGTGCTTCTCCTTGGAATAACATTTTTTCTGGCCACAGAGTTGCTTAATATCATGGAGTATGTGGGGACAATCAATGATGTATCCGGAAGAGCAAGATTGTTTCTAGTTCTTCCTGATGCTTTCTTGGACGCATTTTTGATATTATGGATTTTTACTTCTCTCTCAAAAACACTAGAGCAGCTACAGGCAAAGAGAAGCTCTGTCAAACTGGATATGTATAGGAAGTTCTCAAATGCATTAGCAGTCACAGTCATTGCCTCGGTTGCATGGATAGTGTATGAGGTGTACTTTAAAGCAACAGATCCATTCAATGAGAAGTGGCAGAGTGCTTGGGTCATCACTGCTTTCGGGGATATTCTTGCATTTGCATTGCTGTGTGTGATCTGCTATCTTTGGGCTCCATCTCAGAGTTCTCAACGGTATGCTTACTCAGATGAAGTGGGAGAAGAGTCTGATGACGAAGAATCTCACTCTCTTACGAGGGCCAAGCTAGAGGGTGATATCAGCTTAGTCAAGCAAGACAACATTGAAAGCACAGATTTTGATGAAGATGATACAGAAGACAAGAAGGAATGA

Protein Analysis

348

Amino Acids

39.41

Weight (kDa)

5.18

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 49 - 295 6.7e-90 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01070 AT2G01070
fragaria_vesca FvH4_5g30110 FvH4_5g30110
malus_domestica MD08G1225000.v1.1 MD15G1418500.v1.1
prunus_persica Prupe.1G562600_v2.0.a1
pyrus_communis pycom08g19610 pycom08g19620 pycom15g37150
rosa_chinensis RchiOBHm_Chr7g0224311 RchiOBHm_Chr7g0224371
rosa_laevigata RLG00000001905
rosa_multiflora Rmu_sc0025639.1_g000001
rosa_roxburghii Rroxscaffold_3G00234870
rosa_rugosa Rorug07G0222600
rosa_samantha Rh7AG380800 Rh7BG358500 Rh7DG367600
rosa_wichuraiana Rw7G031110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 93
AccIII TCCGGA 1 cut(s) 551
AciI CCGC 2 cut(s) 93, 439
AclWI GGATC 2 cut(s) 59, 764
AcoI YGGCCR 1 cut(s) 498
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 1 cut(s) 230
AfaI GTAC 1 cut(s) 755
AfiI CCNNNNNNNGG 4 cut(s) 32, 33, 449, 450
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 3 cut(s) 420, 778, 998
AluBI AGCT 6 cut(s) 263, 377, 649, 666, 958, 975
AluI AGCT 6 cut(s) 263, 377, 649, 666, 958, 975
AlwI GGATC 2 cut(s) 59, 764
Ama87I CYCGRG 1 cut(s) 449
Aor13HI TCCGGA 1 cut(s) 551
AoxI GGCC 5 cut(s) 338, 440, 454, 498, 951
ApeKI GCWGC 1 cut(s) 646
ApoI RAATTY 1 cut(s) 316
AseI ATTAAT 1 cut(s) 149
AspS9I GGNCC 3 cut(s) 441, 454, 951
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 2 cut(s) 451, 977
AvaI CYCGRG 1 cut(s) 449
BalI TGGCCA 1 cut(s) 500
BanII GRGCYC 1 cut(s) 868
BbsI GAAGAC 2 cut(s) 392, 1035
BbvI GCAGC 1 cut(s) 658
BccI CCATC 1 cut(s) 877
BciVI GTATCC 1 cut(s) 559
BcnI CCSGG 1 cut(s) 132
BfaI CTAG 3 cut(s) 572, 641, 959
BfmI CTRYAG 1 cut(s) 650
BfuI GTATCC 1 cut(s) 559
BisI GCNGC 2 cut(s) 440, 647
BlsI GCNGC 2 cut(s) 441, 648
Bme1390I CCNGG 1 cut(s) 132
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 3 cut(s) 441, 454, 951
BmiI GGNNCC 3 cut(s) 144, 455, 867
BmrFI CCNGG 1 cut(s) 132
BmrI ACTGGG 1 cut(s) 42
BmsI GCATC 2 cut(s) 264, 574
BmuI ACTGGG 1 cut(s) 42
BpiI GAAGAC 2 cut(s) 392, 1035
BpuMI CCSGG 1 cut(s) 132
BsaAI YACGTR 2 cut(s) 184, 313
BsaBI GATNNNNATC 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 478, 726
BsaWI WCCGGW 1 cut(s) 551
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 33, 449, 450
Bse1I ACTGG 2 cut(s) 37, 681
Bse3DI GCAATG 2 cut(s) 720, 836
Bse8I GATNNNNATC 1 cut(s) 20
BseAI TCCGGA 1 cut(s) 551
BseDI CCNNGG 2 cut(s) 478, 726
BseGI GGATG 2 cut(s) 42, 339
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 4 cut(s) 32, 33, 449, 450
BseMI GCAATG 2 cut(s) 720, 836
BseMII CTCAG 3 cut(s) 224, 887, 912
BseNI ACTGG 2 cut(s) 37, 681
BseXI GCAGC 1 cut(s) 658
BshFI GGCC 5 cut(s) 340, 442, 456, 500, 953
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 2 cut(s) 131, 552
BslFI GGGAC 2 cut(s) 93, 547
BslI CCNNNNNNNGG 4 cut(s) 32, 33, 449, 450
BsmFI GGGAC 2 cut(s) 93, 547
BsnI GGCC 5 cut(s) 340, 442, 456, 500, 953
BsoBI CYCGRG 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 868
Bsp13I TCCGGA 1 cut(s) 551
Bsp143I GATC 3 cut(s) 64, 769, 849
BspACI CCGC 2 cut(s) 93, 439
BspANI GGCC 5 cut(s) 340, 442, 456, 500, 953
BspCNI CTCAG 3 cut(s) 223, 886, 911
BspEI TCCGGA 1 cut(s) 551
BspLI GGNNCC 3 cut(s) 144, 455, 867
BspPI GGATC 2 cut(s) 59, 764
BspQI GCTCTTC 1 cut(s) 550
BsrBI CCGCTC 1 cut(s) 93
BsrDI GCAATG 2 cut(s) 720, 836
BsrI ACTGG 2 cut(s) 37, 681
BssECI CCNNGG 2 cut(s) 478, 726
BssMI GATC 3 cut(s) 64, 769, 849
BssT1I CCWWGG 1 cut(s) 478
Bst4CI ACNGT 4 cut(s) 370, 391, 718, 888
Bst6I CTCTTC 2 cut(s) 550, 909
BstBAI YACGTR 2 cut(s) 184, 313
BstDEI CTNAG 4 cut(s) 210, 873, 898, 976
BstF5I GGATG 2 cut(s) 42, 339
BstKTI GATC 3 cut(s) 67, 772, 852
BstMBI GATC 3 cut(s) 64, 769, 849
BstMWI GCNNNNNNNGC 3 cut(s) 247, 269, 731
BstNSI RCATGY 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 130
BstSFI CTRYAG 1 cut(s) 650
BstSNI TACGTA 1 cut(s) 313
BstV1I GCAGC 1 cut(s) 658
BstV2I GAAGAC 2 cut(s) 392, 1035
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
BsuI GTATCC 1 cut(s) 559
BsuRI GGCC 5 cut(s) 340, 442, 456, 500, 953
BtsCI GGATG 2 cut(s) 42, 339
BtsI GCAGTG 2 cut(s) 250, 807
BtsIMutI CAGTG 2 cut(s) 250, 807
Cfr13I GGNCC 3 cut(s) 441, 454, 951
CseI GACGC 1 cut(s) 605
Csp6I GTAC 1 cut(s) 754
CviAII CATG 3 cut(s) 60, 520, 735
CviQI GTAC 1 cut(s) 754
DdeI CTNAG 4 cut(s) 210, 873, 898, 976
DpnI GATC 3 cut(s) 66, 771, 851
DpnII GATC 3 cut(s) 64, 769, 849
DraI TTTAAA 1 cut(s) 760
EaeI YGGCCR 1 cut(s) 498
Eam1104I CTCTTC 2 cut(s) 550, 909
EarI CTCTTC 2 cut(s) 550, 909
Eco105I TACGTA 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 478
Eco24I GRGCYC 1 cut(s) 868
Eco32I GATATC 1 cut(s) 970
Eco57I CTGAAG 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 454
EcoRV GATATC 1 cut(s) 970
EcoT14I CCWWGG 1 cut(s) 478
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 868
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 3 cut(s) 63, 523, 738
FaqI GGGAC 2 cut(s) 93, 547
FatI CATG 3 cut(s) 59, 519, 734
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 2 cut(s) 440, 647
FokI GGATG 2 cut(s) 49, 346
FriOI GRGCYC 1 cut(s) 868
Fsp4HI GCNGC 2 cut(s) 440, 647
FspBI CTAG 3 cut(s) 572, 641, 959
GluI GCNGC 2 cut(s) 440, 647
HaeIII GGCC 5 cut(s) 340, 442, 456, 500, 953
HapII CCGG 2 cut(s) 131, 552
HgaI GACGC 1 cut(s) 605
Hin1II CATG 3 cut(s) 63, 523, 738
HinfI GANTC 3 cut(s) 292, 917, 932
HpaII CCGG 2 cut(s) 131, 552
HphI GGTGA 2 cut(s) 451, 977
Hpy166II GTNNAC 2 cut(s) 207, 754
Hpy188I TCNGA 3 cut(s) 876, 901, 922
Hpy188III TCNNGA 3 cut(s) 11, 552, 581
Hpy8I GTNNAC 2 cut(s) 207, 754
HpyAV CCTTC 2 cut(s) 223, 1030
HpyCH4III ACNGT 4 cut(s) 370, 391, 718, 888
HpyCH4IV ACGT 2 cut(s) 183, 312
HpyCH4V TGCA 6 cut(s) 241, 255, 704, 734, 830, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 247, 269, 731
HpyF3I CTNAG 4 cut(s) 210, 873, 898, 976
HpySE526I ACGT 2 cut(s) 183, 312
Hsp92II CATG 3 cut(s) 63, 523, 738
Kpn2I TCCGGA 1 cut(s) 551
Kzo9I GATC 3 cut(s) 64, 769, 849
LguI GCTCTTC 1 cut(s) 550
LmnI GCTCC 3 cut(s) 148, 374, 871
LpnPI CCDG 8 cut(s) 18, 144, 315, 482, 565, 594, 638, 662
Lsp1109I GCAGC 1 cut(s) 658
LweI GCATC 2 cut(s) 264, 574
MaeI CTAG 3 cut(s) 572, 641, 959
MaeII ACGT 2 cut(s) 183, 312
MaeIII GTNAC 3 cut(s) 349, 364, 712
MalI GATC 3 cut(s) 66, 771, 851
MbiI CCGCTC 1 cut(s) 93
MboI GATC 3 cut(s) 64, 769, 849
MboII GAAGA 7 cut(s) 392, 567, 569, 926, 941, 1028, 1040
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 1 cut(s) 868
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 3 cut(s) 198, 316, 342
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 1 cut(s) 926
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 9 cut(s) 213, 330, 419, 467, 472, 736, 742, 942, 955
Mox20I TGGCCA 1 cut(s) 500
Mph1103I ATGCAT 1 cut(s) 706
MroI TCCGGA 1 cut(s) 551
MscI TGGCCA 1 cut(s) 500
MseI TTAA 5 cut(s) 20, 149, 321, 513, 759
MslI CAYNNNNRTG 1 cut(s) 841
Msp20I TGGCCA 1 cut(s) 500
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 2 cut(s) 131, 552
MspR9I CCNGG 1 cut(s) 132
MunI CAATTG 1 cut(s) 342
MwoI GCNNNNNNNGC 3 cut(s) 247, 269, 731
NciI CCSGG 1 cut(s) 132
NdeII GATC 3 cut(s) 64, 769, 849
NlaIII CATG 3 cut(s) 63, 523, 738
NlaIV GGNNCC 3 cut(s) 144, 455, 867
NmuCI GTSAC 2 cut(s) 364, 712
NsiI ATGCAT 1 cut(s) 706
NspI RCATGY 1 cut(s) 63
PciSI GCTCTTC 1 cut(s) 550
PfeI GAWTC 2 cut(s) 292, 932
PkrI GCNGC 2 cut(s) 441, 648
PleI GAGTC 1 cut(s) 925
PpsI GAGTC 1 cut(s) 925
Ppu21I YACGTR 2 cut(s) 184, 313
PshBI ATTAAT 1 cut(s) 149
PspN4I GGNNCC 3 cut(s) 144, 455, 867
PspPI GGNCC 3 cut(s) 441, 454, 951
PsrI GAACNNNNNNTAC 2 cut(s) 677, 709
PsuI RGATCY 1 cut(s) 769
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 755
RsaNI GTAC 1 cut(s) 754
RseI CAYNNNNRTG 1 cut(s) 841
SapI GCTCTTC 1 cut(s) 550
SaqAI TTAA 5 cut(s) 20, 149, 321, 513, 759
SatI GCNGC 2 cut(s) 440, 647
Sau3AI GATC 3 cut(s) 64, 769, 849
Sau96I GGNCC 3 cut(s) 441, 454, 951
SchI GAGTC 1 cut(s) 926
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 1 cut(s) 868
SfaNI GCATC 2 cut(s) 264, 574
SfcI CTRYAG 1 cut(s) 650
SmiMI CAYNNNNRTG 1 cut(s) 841
SnaBI TACGTA 1 cut(s) 313
Sse9I AATT 3 cut(s) 198, 316, 342
SsiI CCGC 2 cut(s) 93, 439
SspMI CTAG 3 cut(s) 572, 641, 959
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 1 cut(s) 478
TaaI ACNGT 4 cut(s) 370, 391, 718, 888
TaiI ACGT 2 cut(s) 186, 315
TaqI TCGA 1 cut(s) 136
TasI AATT 3 cut(s) 198, 316, 342
TatI WGTACW 1 cut(s) 753
TauI GCSGC 1 cut(s) 442
TfiI GAWTC 2 cut(s) 292, 932
Tru1I TTAA 5 cut(s) 20, 149, 321, 513, 759
Tru9I TTAA 5 cut(s) 20, 149, 321, 513, 759
TscAI CASTG 2 cut(s) 257, 814
TseFI GTSAC 2 cut(s) 364, 712
TseI GCWGC 1 cut(s) 646
Tsp45I GTSAC 2 cut(s) 364, 712
TspDTI ATGAA 3 cut(s) 167, 918, 1029
TspRI CASTG 2 cut(s) 257, 814
VspI ATTAAT 1 cut(s) 149
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 63
XspI CTAG 3 cut(s) 572, 641, 959
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.