pycom08g21100

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008685
Physical Location & Seq
Reverse (-)
20683650 .. 20685758
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
ATGAAAAAACAATTGAGCAAAGCCTCTGCTCAATTATCAGCTTCTTCTCACAAAAATAAAGAAGCTGCTGCTCCTGCTTCTGCAATTGTCCCTGCTTCTACCGCTGCTGAATTCTTGCCATTAGAAGGCGGTCCAGCGCGGAAACTCCCCCAAGAAAAGCCCACGGAGGATGTTGCTACTGTTTTATACATTGGTCGGATACCTCATGGCTTCTATGAGAAAGAAATGGAAGGATTTTTTGGGCAATTTGGTTCGATTAAGAGATTAAGAATTGCGCGTAACAAAAAGACAGGGAAATCAAAGCATTTTGGCTTCATTGAGTTTGAAGAACCTCAGGTGGCGAAAGTTGTAGCTGACACTATGCATAACTATTTGTTGTTCGAACACGTGTTGCAAGTCCATCTTATTCCTCCGCAGAAAGTTCACCCGAAATTATGGAAAGGTTTCAACTACCGAGTCAGACCCGTGAACTGGGTTCAAATTGAGCGGAAGAGACAAGACAAGGAAAGAACAGTGGAAGAGCACAAGAAGTTAATGGGGAAGATTGTGAAGAGAGATCTGAAGCGTCAAAAGAAAATTGAGGCTGCTGGTATTGATTATAAATGCCCAGAAATTGTGGGTAGTAGTGAGGAGCCTGCTCCAAAGAAAAGGAAGACCGACCGAAAGAAGGTTGTTTCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

228

Amino Acids

25.87

Weight (kDa)

10.0

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 62 - 131 9.7e-14 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
AccBSI CCGCTC 1 cut(s) 487
AccII CGCG 2 cut(s) 139, 277
AciI CCGC 5 cut(s) 102, 129, 139, 413, 487
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 581
AcvI CACGTG 1 cut(s) 388
AfiI CCNNNNNNNGG 3 cut(s) 125, 471, 667
AflIII ACRYGT 2 cut(s) 385, 387
AgsI TTSAA 3 cut(s) 326, 448, 479
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AluBI AGCT 3 cut(s) 41, 65, 353
AluI AGCT 3 cut(s) 41, 65, 353
Alw21I GWGCWC 1 cut(s) 525
Alw26I GTCTC 1 cut(s) 487
ApeKI GCWGC 4 cut(s) 65, 68, 104, 584
ApoI RAATTY 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 2 cut(s) 139, 277
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 1 cut(s) 416
AsuII TTCGAA 1 cut(s) 381
AvaII GGWCC 1 cut(s) 131
AxyI CCTNAGG 1 cut(s) 333
BbrPI CACGTG 1 cut(s) 388
BbsI GAAGAC 1 cut(s) 659
Bbv12I GWGCWC 1 cut(s) 525
BbvI GCAGC 4 cut(s) 52, 55, 91, 571
BccI CCATC 1 cut(s) 408
BciVI GTATCC 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 487
BfuI GTATCC 1 cut(s) 192
BglII AGATCT 1 cut(s) 556
BisI GCNGC 4 cut(s) 66, 69, 105, 585
BlsI GCNGC 4 cut(s) 67, 70, 106, 586
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 633
BmrI ACTGGG 1 cut(s) 481
BmuI ACTGGG 1 cut(s) 481
BpiI GAAGAC 1 cut(s) 659
Bpu14I TTCGAA 1 cut(s) 381
BsaAI YACGTR 1 cut(s) 388
BsaJI CCNNGG 1 cut(s) 162
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 471, 667
Bse1I ACTGG 1 cut(s) 476
Bse21I CCTNAGG 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 3 cut(s) 125, 471, 667
BseMII CTCAG 1 cut(s) 347
BseNI ACTGG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 644
BseXI GCAGC 4 cut(s) 52, 55, 91, 571
Bsh1236I CGCG 2 cut(s) 139, 277
Bsh1285I CGRYCG 1 cut(s) 661
BsiEI CGRYCG 1 cut(s) 661
BsiHKAI GWGCWC 1 cut(s) 525
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 3 cut(s) 125, 471, 667
BsmAI GTCTC 1 cut(s) 487
BsmFI GGGAC 1 cut(s) 74
Bsp119I TTCGAA 1 cut(s) 381
Bsp1286I GDGCHC 1 cut(s) 525
Bsp143I GATC 1 cut(s) 556
BspACI CCGC 5 cut(s) 102, 129, 139, 413, 487
BspCNI CTCAG 1 cut(s) 346
BspFNI CGCG 2 cut(s) 139, 277
BspLI GGNNCC 1 cut(s) 633
BspQI GCTCTTC 1 cut(s) 513
BspT104I TTCGAA 1 cut(s) 381
BsrBI CCGCTC 1 cut(s) 487
BsrI ACTGG 1 cut(s) 476
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 556
Bst4CI ACNGT 2 cut(s) 181, 514
Bst6I CTCTTC 3 cut(s) 485, 513, 545
BstBAI YACGTR 1 cut(s) 388
BstBI TTCGAA 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 636
BstDEI CTNAG 1 cut(s) 333
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 2 cut(s) 139, 277
BstHHI GCGC 2 cut(s) 139, 277
BstKTI GATC 1 cut(s) 559
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 1 cut(s) 556
BstMCI CGRYCG 1 cut(s) 661
BstMWI GCNNNNNNNGC 2 cut(s) 74, 101
BstUI CGCG 2 cut(s) 139, 277
BstV1I GCAGC 4 cut(s) 52, 55, 91, 571
BstV2I GAAGAC 1 cut(s) 659
BstX2I RGATCY 1 cut(s) 556
BstYI RGATCY 1 cut(s) 556
Bsu36I CCTNAGG 1 cut(s) 333
BsuI GTATCC 1 cut(s) 192
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 1 cut(s) 636
CfoI GCGC 2 cut(s) 139, 277
Cfr13I GGNCC 1 cut(s) 131
CseI GACGC 1 cut(s) 554
CviAII CATG 1 cut(s) 206
CviJI RGCY 9 cut(s) 23, 41, 65, 160, 210, 312, 353, 584, 634
CviKI_1 RGCY 9 cut(s) 23, 41, 65, 160, 210, 312, 353, 584, 634
DdeI CTNAG 1 cut(s) 333
DpnI GATC 1 cut(s) 558
DpnII GATC 1 cut(s) 556
Eam1104I CTCTTC 3 cut(s) 485, 513, 545
EarI CTCTTC 3 cut(s) 485, 513, 545
Eco47I GGWCC 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 581
Eco72I CACGTG 1 cut(s) 388
Eco81I CCTNAGG 1 cut(s) 333
EcoRI GAATTC 1 cut(s) 110
EcoT22I ATGCAT 1 cut(s) 366
FaeI CATG 1 cut(s) 209
FaiI YATR 7 cut(s) 187, 207, 216, 362, 366, 436, 600
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FaqI GGGAC 1 cut(s) 74
FatI CATG 1 cut(s) 205
Fnu4HI GCNGC 4 cut(s) 66, 69, 105, 585
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 4 cut(s) 66, 69, 105, 585
GlaI GCGC 2 cut(s) 138, 276
GluI GCNGC 4 cut(s) 66, 69, 105, 585
HgaI GACGC 1 cut(s) 554
HhaI GCGC 2 cut(s) 139, 277
Hin1II CATG 1 cut(s) 209
Hin6I GCGC 2 cut(s) 137, 275
HinP1I GCGC 2 cut(s) 137, 275
HinfI GANTC 1 cut(s) 456
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 2 cut(s) 424, 469
Hpy188I TCNGA 3 cut(s) 198, 461, 561
Hpy8I GTNNAC 2 cut(s) 424, 469
HpyAV CCTTC 3 cut(s) 119, 224, 661
HpyCH4III ACNGT 2 cut(s) 181, 514
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 3 cut(s) 83, 364, 394
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 101
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 1 cut(s) 209
HspAI GCGC 2 cut(s) 137, 275
Kzo9I GATC 1 cut(s) 556
LguI GCTCTTC 1 cut(s) 513
LmnI GCTCC 3 cut(s) 76, 631, 643
LpnPI CCDG 9 cut(s) 87, 105, 147, 276, 320, 457, 573, 621, 648
Lsp1109I GCAGC 4 cut(s) 52, 55, 91, 571
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 1 cut(s) 278
MalI GATC 1 cut(s) 558
MbiI CCGCTC 1 cut(s) 487
MboI GATC 1 cut(s) 556
MboII GAAGA 7 cut(s) 36, 338, 502, 530, 553, 562, 664
MfeI CAATTG 2 cut(s) 11, 84
MflI RGATCY 1 cut(s) 556
MhlI GDGCHC 1 cut(s) 525
MlyI GAGTC 1 cut(s) 465
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 7 cut(s) 34, 160, 213, 342, 420, 574, 622
Mph1103I ATGCAT 1 cut(s) 366
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 3 cut(s) 258, 266, 533
MspA1I CMGCKG 1 cut(s) 104
MunI CAATTG 2 cut(s) 11, 84
MvnI CGCG 2 cut(s) 139, 277
MwoI GCNNNNNNNGC 2 cut(s) 74, 101
NdeII GATC 1 cut(s) 556
NlaIII CATG 1 cut(s) 209
NlaIV GGNNCC 1 cut(s) 633
NsiI ATGCAT 1 cut(s) 366
NspV TTCGAA 1 cut(s) 381
PciSI GCTCTTC 1 cut(s) 513
PdmI GAANNNNTTC 1 cut(s) 443
PkrI GCNGC 4 cut(s) 67, 70, 106, 586
PleI GAGTC 1 cut(s) 464
PmaCI CACGTG 1 cut(s) 388
PmlI CACGTG 1 cut(s) 388
PpsI GAGTC 1 cut(s) 464
Ppu21I YACGTR 1 cut(s) 388
PsiI TTATAA 1 cut(s) 600
PspCI CACGTG 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 633
PspPI GGNCC 1 cut(s) 131
PsuI RGATCY 1 cut(s) 556
SapI GCTCTTC 1 cut(s) 513
SaqAI TTAA 3 cut(s) 258, 266, 533
SatI GCNGC 4 cut(s) 66, 69, 105, 585
Sau3AI GATC 1 cut(s) 556
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 1 cut(s) 465
SduI GDGCHC 1 cut(s) 525
SetI ASST 9 cut(s) 43, 67, 205, 334, 339, 355, 390, 445, 672
SfuI TTCGAA 1 cut(s) 381
SinI GGWCC 1 cut(s) 131
SsiI CCGC 5 cut(s) 102, 129, 139, 413, 487
TaaI ACNGT 2 cut(s) 181, 514
TaiI ACGT 1 cut(s) 390
TaqI TCGA 2 cut(s) 254, 381
TaqII GACCGA 2 cut(s) 671, 675
Tru1I TTAA 3 cut(s) 258, 266, 533
Tru9I TTAA 3 cut(s) 258, 266, 533
TscAI CASTG 1 cut(s) 519
TseI GCWGC 4 cut(s) 65, 68, 104, 584
TspDTI ATGAA 2 cut(s) 17, 304
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 1 cut(s) 519
VpaK11BI GGWCC 1 cut(s) 131
XapI RAATTY 1 cut(s) 110
XmnI GAANNNNTTC 1 cut(s) 443
Zsp2I ATGCAT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.