pycom08g21660
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010263
Physical Location & Seq
Reverse (-)
21083876 .. 21085558
1683 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 786 bp
ATGCCCAATCTAGTTGCAGAAACAACAACAACCTCATCATCATCAACAATGAGCCACGACGACTCCAACAAGGCCGACCATGAGCATGAGCATGATGAGTATGTTGATGAGCATGAGCACGACATGGTGATGCCGGGCTTTCGCTTCCACCCTACTGAGGAGGAACTTGTAGAGTTCTACCTTCGCCGTAAGGTTGAGGGCAAGCGCTTCAATGTTGAACTCATTACTTTTCTCGATCTTTATCGCTATGACCCTTGGGAACTTCCAGCTATGGCAGCAATTGGGGAGAAGGAATGGTTCTTTTATGTGCCAAGAGACCGCAAGTACCGCAACGGCGATAGGCCAAACCGGGTAACTACTTCTGGGTATTGGAAGGCAACTGGAGCCGACCGCATGATTCGAAGCGAAAACTTCCGCTCAATCGGCCTCAAGAAAACCCTAGTCTTTTACTCCGGGAAAGCTCCCAAAGGCATTCGAACTAGTTGGATCATGAACGAATATCGCTTGCCGCATCATGAAACTGAACGATATCAAAAGGGAGAAATATCACTATGCCGAGTATACAAGAGAGCCGGAGTTGAAGACCACCCATCACTCCCTCGTTGTCTCCCAGCATCCAGGGCATCCACATCTAGATCATCAGTACTAGTGGCTCAATCGACGATCAGGTCAGACAATAAAAAGCAGCACACCAATAATATCAACACAGTTGATAGTGTGATTGAAAAACTCCAAGCTTTTAATGAAGGCCAATCGCAGCAGATTCATCATGATCGATCTAGGTGA

Protein Analysis

262

Amino Acids

30.49

Weight (kDa)

7.84

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 45 - 169 1.8e-45 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 667
AccBSI CCGCTC 1 cut(s) 417
AccI GTMKAC 1 cut(s) 561
AciI CCGC 5 cut(s) 319, 328, 391, 415, 509
AclWI GGATC 1 cut(s) 494
AfaI GTAC 2 cut(s) 326, 645
AfeI AGCGCT 1 cut(s) 206
AfiI CCNNNNNNNGG 1 cut(s) 157
AgsI TTSAA 4 cut(s) 211, 218, 581, 725
AhlI ACTAGT 2 cut(s) 479, 646
AjnI CCWGG 1 cut(s) 617
AluBI AGCT 3 cut(s) 269, 461, 737
AluI AGCT 3 cut(s) 269, 461, 737
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 2 cut(s) 309, 611
AlwI GGATC 1 cut(s) 494
Aor51HI AGCGCT 1 cut(s) 206
AoxI GGCC 4 cut(s) 72, 341, 424, 748
ApeKI GCWGC 3 cut(s) 275, 685, 757
AspLEI GCGC 1 cut(s) 207
AsuC2I CCSGG 3 cut(s) 135, 350, 454
AsuHPI GGTGA 1 cut(s) 139
AsuII TTCGAA 2 cut(s) 400, 475
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 3 cut(s) 287, 697, 769
BccI CCATC 1 cut(s) 598
BceAI ACGGC 2 cut(s) 171, 349
BciT130I CCWGG 1 cut(s) 619
BcnI CCSGG 3 cut(s) 135, 350, 454
BcoDI GTCTC 2 cut(s) 309, 611
BcuI ACTAGT 2 cut(s) 479, 646
BfaI CTAG 6 cut(s) 11, 440, 480, 633, 647, 780
BfoI RGCGCY 1 cut(s) 208
BisI GCNGC 4 cut(s) 276, 509, 686, 758
BlsI GCNGC 4 cut(s) 277, 510, 687, 759
BmcAI AGTACT 1 cut(s) 645
Bme1390I CCNGG 4 cut(s) 135, 350, 454, 619
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 4 cut(s) 135, 350, 454, 619
BmsI GCATC 4 cut(s) 120, 520, 623, 632
BpiI GAAGAC 1 cut(s) 588
BpmI CTGGAG 1 cut(s) 402
Bpu14I TTCGAA 2 cut(s) 400, 475
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 3 cut(s) 135, 350, 454
Bsa29I ATCGAT 1 cut(s) 775
BsaBI GATNNNNATC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 254, 618
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 385
Bse8I GATNNNNATC 1 cut(s) 240
BseBI CCWGG 1 cut(s) 619
BseCI ATCGAT 1 cut(s) 775
BseDI CCNNGG 2 cut(s) 254, 618
BseGI GGATG 2 cut(s) 614, 623
BseJI GATNNNNATC 1 cut(s) 240
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 3 cut(s) 287, 697, 769
BseYI CCCAGC 1 cut(s) 610
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 4 cut(s) 74, 343, 426, 750
BshVI ATCGAT 1 cut(s) 775
BsiEI CGRYCG 1 cut(s) 391
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 4 cut(s) 134, 349, 453, 573
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 2 cut(s) 309, 611
BsmI GAATGC 1 cut(s) 471
BsnI GGCC 4 cut(s) 74, 343, 426, 750
Bso31I GGTCTC 1 cut(s) 309
Bsp119I TTCGAA 2 cut(s) 400, 475
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 6 cut(s) 235, 486, 635, 663, 772, 776
BspACI CCGC 5 cut(s) 319, 328, 391, 415, 509
BspANI GGCC 4 cut(s) 74, 343, 426, 750
BspCNI CTCAG 1 cut(s) 148
BspDI ATCGAT 1 cut(s) 775
BspHI TCATGA 3 cut(s) 489, 514, 769
BspLI GGNNCC 1 cut(s) 385
BspPI GGATC 1 cut(s) 494
BspT104I TTCGAA 2 cut(s) 400, 475
BspTNI GGTCTC 1 cut(s) 309
BsrBI CCGCTC 1 cut(s) 417
BsrI ACTGG 1 cut(s) 385
BssECI CCNNGG 2 cut(s) 254, 618
BssMI GATC 6 cut(s) 235, 486, 635, 663, 772, 776
BssNAI GTATAC 1 cut(s) 562
BssT1I CCWWGG 1 cut(s) 254
Bst1107I GTATAC 1 cut(s) 562
Bst2UI CCWGG 1 cut(s) 619
Bst4CI ACNGT 1 cut(s) 709
BstBI TTCGAA 2 cut(s) 400, 475
BstC8I GCNNGC 2 cut(s) 203, 506
BstDEI CTNAG 1 cut(s) 156
BstF5I GGATG 2 cut(s) 614, 623
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 6 cut(s) 238, 489, 638, 666, 775, 779
BstMAI GTCTC 2 cut(s) 309, 611
BstMBI GATC 6 cut(s) 235, 486, 635, 663, 772, 776
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 5 cut(s) 275, 327, 383, 423, 620
BstNI CCWGG 1 cut(s) 619
BstSCI CCNGG 4 cut(s) 133, 348, 452, 617
BstV1I GCAGC 3 cut(s) 287, 697, 769
BstV2I GAAGAC 1 cut(s) 588
BstZ17I GTATAC 1 cut(s) 562
Bsu15I ATCGAT 1 cut(s) 775
BsuRI GGCC 4 cut(s) 74, 343, 426, 750
BsuTUI ATCGAT 1 cut(s) 775
BtsCI GGATG 2 cut(s) 614, 623
Cac8I GCNNGC 2 cut(s) 203, 506
CciI TCATGA 3 cut(s) 489, 514, 769
CfoI GCGC 1 cut(s) 207
ClaI ATCGAT 1 cut(s) 775
Csp6I GTAC 2 cut(s) 325, 644
CviAII CATG 9 cut(s) 80, 86, 92, 113, 124, 394, 490, 515, 770
CviQI GTAC 2 cut(s) 325, 644
DdeI CTNAG 1 cut(s) 156
DpnI GATC 6 cut(s) 237, 488, 637, 665, 774, 778
DpnII GATC 6 cut(s) 235, 486, 635, 663, 772, 776
DrdI GACNNNNNNGTC 1 cut(s) 667
DseDI GACNNNNNNGTC 1 cut(s) 667
Eco130I CCWWGG 1 cut(s) 254
Eco31I GGTCTC 1 cut(s) 309
Eco32I GATATC 1 cut(s) 530
Eco47III AGCGCT 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 617
EcoRV GATATC 1 cut(s) 530
EcoT14I CCWWGG 1 cut(s) 254
ErhI CCWWGG 1 cut(s) 254
FaeI CATG 9 cut(s) 83, 89, 95, 116, 127, 397, 493, 518, 773
FatI CATG 9 cut(s) 79, 85, 91, 112, 123, 393, 489, 514, 769
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 4 cut(s) 276, 509, 686, 758
FokI GGATG 2 cut(s) 601, 610
Fsp4HI GCNGC 4 cut(s) 276, 509, 686, 758
FspBI CTAG 6 cut(s) 11, 440, 480, 633, 647, 780
GlaI GCGC 1 cut(s) 206
GluI GCNGC 4 cut(s) 276, 509, 686, 758
GsaI CCCAGC 1 cut(s) 614
GsuI CTGGAG 1 cut(s) 402
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 4 cut(s) 74, 343, 426, 750
HapII CCGG 4 cut(s) 134, 349, 453, 573
HhaI GCGC 1 cut(s) 207
Hin1II CATG 9 cut(s) 83, 89, 95, 116, 127, 397, 493, 518, 773
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 735
HinfI GANTC 3 cut(s) 62, 397, 763
HpaII CCGG 4 cut(s) 134, 349, 453, 573
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 1 cut(s) 673
Hpy188III TCNNGA 6 cut(s) 233, 430, 490, 515, 633, 770
Hpy8I GTNNAC 1 cut(s) 562
Hpy99I CGWCG 2 cut(s) 62, 664
HpyAV CCTTC 4 cut(s) 191, 283, 367, 740
HpyCH4III ACNGT 1 cut(s) 709
HpyCH4V TGCA 1 cut(s) 17
HpyF10VI GCNNNNNNNGC 5 cut(s) 275, 327, 383, 423, 620
HpyF3I CTNAG 1 cut(s) 156
Hsp92II CATG 9 cut(s) 83, 89, 95, 116, 127, 397, 493, 518, 773
HspAI GCGC 1 cut(s) 205
Kzo9I GATC 6 cut(s) 235, 486, 635, 663, 772, 776
LmnI GCTCC 2 cut(s) 383, 466
Lsp1109I GCAGC 3 cut(s) 287, 697, 769
LweI GCATC 4 cut(s) 120, 520, 623, 632
MaeI CTAG 6 cut(s) 11, 440, 480, 633, 647, 780
MaeIII GTNAC 1 cut(s) 352
MalI GATC 6 cut(s) 237, 488, 637, 665, 774, 778
MbiI CCGCTC 1 cut(s) 417
MboI GATC 6 cut(s) 235, 486, 635, 663, 772, 776
MboII GAAGA 1 cut(s) 593
MfeI CAATTG 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 1 cut(s) 279
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 2 cut(s) 90, 464
MnlI CCTC 6 cut(s) 43, 151, 154, 190, 437, 609
MseI TTAA 1 cut(s) 741
MslI CAYNNNNRTG 3 cut(s) 84, 90, 128
MspI CCGG 4 cut(s) 134, 349, 453, 573
MspR9I CCNGG 4 cut(s) 135, 350, 454, 619
MunI CAATTG 1 cut(s) 279
Mva1269I GAATGC 1 cut(s) 471
MvaI CCWGG 1 cut(s) 619
MwoI GCNNNNNNNGC 5 cut(s) 275, 327, 383, 423, 620
NciI CCSGG 3 cut(s) 135, 350, 454
NdeII GATC 6 cut(s) 235, 486, 635, 663, 772, 776
NlaIII CATG 9 cut(s) 83, 89, 95, 116, 127, 397, 493, 518, 773
NlaIV GGNNCC 1 cut(s) 385
NmeAIII GCCGAG 1 cut(s) 581
NspV TTCGAA 2 cut(s) 400, 475
PagI TCATGA 3 cut(s) 489, 514, 769
PctI GAATGC 1 cut(s) 471
PfeI GAWTC 2 cut(s) 397, 763
PfoI TCCNGGA 1 cut(s) 452
PkrI GCNGC 4 cut(s) 277, 510, 687, 759
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 617
PspFI CCCAGC 1 cut(s) 610
PspGI CCWGG 1 cut(s) 617
PspN4I GGNNCC 1 cut(s) 385
RsaI GTAC 2 cut(s) 326, 645
RsaNI GTAC 2 cut(s) 325, 644
RseI CAYNNNNRTG 3 cut(s) 84, 90, 128
SaqAI TTAA 1 cut(s) 741
SatI GCNGC 4 cut(s) 276, 509, 686, 758
Sau3AI GATC 6 cut(s) 235, 486, 635, 663, 772, 776
ScaI AGTACT 1 cut(s) 645
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 4 cut(s) 135, 350, 454, 619
SduI GDGCHC 1 cut(s) 120
SetI ASST 8 cut(s) 35, 183, 195, 271, 463, 671, 739, 785
SfaNI GCATC 4 cut(s) 120, 520, 623, 632
SfuI TTCGAA 2 cut(s) 400, 475
SmiMI CAYNNNNRTG 3 cut(s) 84, 90, 128
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
SpeI ACTAGT 2 cut(s) 479, 646
Sse9I AATT 1 cut(s) 279
SsiI CCGC 5 cut(s) 319, 328, 391, 415, 509
SspMI CTAG 6 cut(s) 11, 440, 480, 633, 647, 780
StyD4I CCNGG 4 cut(s) 133, 348, 452, 617
StyI CCWWGG 1 cut(s) 254
TaaI ACNGT 1 cut(s) 709
TaqI TCGA 5 cut(s) 234, 400, 475, 659, 775
TasI AATT 1 cut(s) 279
TatI WGTACW 1 cut(s) 643
TauI GCSGC 1 cut(s) 511
TfiI GAWTC 2 cut(s) 397, 763
Tru1I TTAA 1 cut(s) 741
Tru9I TTAA 1 cut(s) 741
TseI GCWGC 3 cut(s) 275, 685, 757
TspDTI ATGAA 4 cut(s) 506, 531, 755, 759
XbaI TCTAGA 1 cut(s) 632
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 6 cut(s) 11, 440, 480, 633, 647, 780
ZrmI AGTACT 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.