pycom09g02200

Equilibrative nucleotide transporter 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0008216
Physical Location & Seq
Reverse (-)
1659443 .. 1661907
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1605 bp
ATGGCAAGCCCTGATGTAAATGCACCCCCACCAAAGCTTGAGGGGAAGTATGCTGCAATGGTAGTGTGTTGGCTTCTTGGAAATGGATGCCTCTTTTCATGGAACAGCATGCTCACAATTGTAGATTACTATGTTTACTTGTTCCCGGTAACTGCCCGAATAAATCAATTTTTGTTCTCACTATTCCTGTTAGTTCATAATCTCCCGTATTTGCTTTTGAATCATGTGAAAGTAGGCAGTGTGTTTGGACAGTTTGTTACATTTGAAGTTAGATTAAATGCCTCATCCCTTAAGTTTTGTTTCCTTTTTTTCCGGGTGCAGCGGTACCATCCCTCGAGGGTACTTACAGTGGTATACCAGCCATTCGCAGTTTTAACAATCATTGTACTGTCATACCATGAGGCGAAGATCAATACGAGGAAGCGAAACCTATTAGGATACATTCTGTTTTTCTTAAGTTCTCTGTTGGTGCTAGTTTTGGATTTAGCCACATCCGGGAAAGGAGGTCTTGGAACTTTCATTGGAATTTGTGCAATTAGCGCTGTCTTTGGAATTGCAGATGCTCACGTACTAGGAGGAATGGTTGGAGACCTCTCTTTCATGCCAGTAGAGCTCATCCAATATCTCTTGTTTCAAACTTTTCAGTCTTTCCTTGCTGGTTTGGCTGCATCAGGAGCTCTAACCTCTGCTTTGAGGTTGATTACAAAGGCAGCATTTGAGAATTCCCAAAATGGTCTTAGAAAGGGAGCCAGTAGGTTTTTTAGTTCTCAGTTTTTCAAATTTTTTTTGTTCTTTGCCATCACTTCATTCTTCGAGCTTCTCTGCATTCTCCTCTACGCTTTTTCTTTTCCAAAACTGCCAATTGTTAAGTACTATCGCTCTAAGGCAGCTTCCGAAGGATCTAAAACTGTTTCAGCTGACCTTGCAGCTGGAGGCATCCAAACATTTGCTGAAGCAACAGTATGTTATTTTGTCCATTATTTGCTTACAAAGTTTTCCAAGCATTCATGTTCTAATGTTCTGCAAAAAAAACACCCCAATTTGAAGCAGGATGAGGAGAATCTTAGAGAATGTGATCGACTTGGAAACAAACAGTTGTTGCTTCAAAACATTGATTATGCACTTGACATGTTTTTAATCTATACACTCACTTTGTCAATATCCCCCGGATTCTTATCAGAGGACACTGGATCTCACAGTTTGGGAACATGGTATGTAGTGAACATAATCTATCACATGTATGCACTTGTTTTGATTGCACTGTACAATGTGTGTGATCTCATCGGAAGATACATTCCGCTAGTGAAATTCCTGAAGTTGGAATCGCGGAGAGGGCTCATGACCGTGATTCTTTCGCGCTTCTTACTTGTCCCGTGTTTCTACTTCACAGCCAAGTATGGTGACCAAGGCTGGATGATCATGCTGACATCCTTCTTAGGTTTAACCAATGGTTACCTCACCGTCTGCGTCCTTACTTCAGCACCTAAGGGCTACAGGGGGCCGGAGCAAAATGCTTTGGGAAATCTTCTGGTGTTGTTCTTGCTTGGAGGTATACTAGCAGGGGTGGTACTTGACTGGTTGTGGCTTATAGGCAAGGGCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleoside_tran

Protein Analysis

535

Amino Acids

59.78

Weight (kDa)

9.21

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoside_tran PF01733 183 - 528 1.3e-19 Nucleoside transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 334
Acc65I GGTACC 1 cut(s) 324
AccB1I GGYRCC 1 cut(s) 324
AccI GTMKAC 2 cut(s) 354, 1552
AccII CGCG 2 cut(s) 1327, 1357
AciI CCGC 3 cut(s) 322, 1298, 1327
AclWI GGATC 2 cut(s) 907, 1198
AcsI RAATTY 4 cut(s) 525, 721, 779, 1307
AcuI CTGAAG 3 cut(s) 972, 1334, 1461
AfaI GTAC 7 cut(s) 326, 342, 387, 570, 872, 1265, 1569
AfeI AGCGCT 1 cut(s) 541
AfiI CCNNNNNNNGG 2 cut(s) 495, 1318
AflII CTTAAG 2 cut(s) 290, 454
AflIII ACRYGT 2 cut(s) 1128, 1236
AgsI TTSAA 6 cut(s) 220, 266, 635, 778, 1045, 1106
AluBI AGCT 7 cut(s) 37, 613, 677, 817, 890, 917, 929
AluI AGCT 7 cut(s) 37, 613, 677, 817, 890, 917, 929
Alw21I GWGCWC 2 cut(s) 615, 679
Alw26I GTCTC 1 cut(s) 582
AlwI GGATC 2 cut(s) 907, 1198
Ama87I CYCGRG 1 cut(s) 334
Aor51HI AGCGCT 1 cut(s) 541
AoxI GGCC 1 cut(s) 1499
ApeKI GCWGC 6 cut(s) 53, 319, 665, 710, 887, 926
ApoI RAATTY 4 cut(s) 525, 721, 779, 1307
Asp718I GGTACC 1 cut(s) 324
AspLEI GCGC 2 cut(s) 542, 1359
AspS9I GGNCC 1 cut(s) 1499
AsuC2I CCSGG 4 cut(s) 146, 314, 496, 1167
AsuHPI GGTGA 2 cut(s) 1412, 1450
AvaI CYCGRG 1 cut(s) 334
AxyI CCTNAGG 1 cut(s) 1485
BanI GGYRCC 1 cut(s) 324
BanII GRGCYC 3 cut(s) 615, 679, 1338
Bbv12I GWGCWC 2 cut(s) 615, 679
BbvI GCAGC 6 cut(s) 40, 331, 652, 722, 899, 938
BccI CCATC 2 cut(s) 336, 806
BciVI GTATCC 1 cut(s) 431
BclI TGATCA 1 cut(s) 1416
BcnI CCSGG 4 cut(s) 146, 314, 496, 1167
BcoDI GTCTC 1 cut(s) 582
BfaI CTAG 4 cut(s) 473, 572, 1301, 1556
BfmI CTRYAG 1 cut(s) 1492
BfoI RGCGCY 1 cut(s) 543
BfrI CTTAAG 2 cut(s) 290, 454
BfuI GTATCC 1 cut(s) 431
BisI GCNGC 6 cut(s) 54, 320, 666, 711, 888, 927
BlsI GCNGC 6 cut(s) 55, 321, 667, 712, 889, 928
BmcAI AGTACT 1 cut(s) 872
Bme1390I CCNGG 4 cut(s) 146, 314, 496, 1167
BmeT110I CYCGRG 1 cut(s) 334
BmgT120I GGNCC 1 cut(s) 1499
BmiI GGNNCC 3 cut(s) 326, 748, 1500
BmrFI CCNGG 4 cut(s) 146, 314, 496, 1167
BmsI GCATC 4 cut(s) 77, 550, 677, 945
BpmI CTGGAG 1 cut(s) 951
BpuEI CTTGAG 1 cut(s) 59
BpuMI CCSGG 4 cut(s) 146, 314, 496, 1167
BsaAI YACGTR 1 cut(s) 568
BsaBI GATNNNNATC 1 cut(s) 1174
BsaI GGTCTC 1 cut(s) 582
BsaJI CCNNGG 2 cut(s) 1165, 1405
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bsc4I CCNNNNNNNGG 2 cut(s) 495, 1318
Bse1I ACTGG 4 cut(s) 605, 750, 1192, 1580
Bse21I CCTNAGG 1 cut(s) 1485
Bse3DI GCAATG 1 cut(s) 63
Bse8I GATNNNNATC 1 cut(s) 1174
BseDI CCNNGG 2 cut(s) 1165, 1405
BseGI GGATG 9 cut(s) 92, 284, 328, 491, 615, 936, 1057, 1419, 1427
BseJI GATNNNNATC 1 cut(s) 1174
BseLI CCNNNNNNNGG 2 cut(s) 495, 1318
BseMI GCAATG 1 cut(s) 63
BseMII CTCAG 1 cut(s) 782
BseNI ACTGG 4 cut(s) 605, 750, 1192, 1580
BseRI GAGGAG 2 cut(s) 821, 1070
BseXI GCAGC 6 cut(s) 40, 331, 652, 722, 899, 938
BsgI GTGCAG 1 cut(s) 338
Bsh1236I CGCG 2 cut(s) 1327, 1357
BshFI GGCC 1 cut(s) 1501
BshNI GGYRCC 1 cut(s) 324
BsiHKAI GWGCWC 2 cut(s) 615, 679
BsiHKCI CYCGRG 1 cut(s) 334
BsiSI CCGG 5 cut(s) 146, 313, 495, 1167, 1502
BslFI GGGAC 1 cut(s) 1355
BslI CCNNNNNNNGG 2 cut(s) 495, 1318
BsmAI GTCTC 1 cut(s) 582
BsmFI GGGAC 1 cut(s) 1355
BsmI GAATGC 2 cut(s) 825, 1003
BsnI GGCC 1 cut(s) 1501
Bso31I GGTCTC 1 cut(s) 582
BsoBI CYCGRG 1 cut(s) 334
Bsp1286I GDGCHC 3 cut(s) 615, 679, 1338
Bsp1407I TGTACA 1 cut(s) 1263
Bsp143I GATC 6 cut(s) 408, 899, 1075, 1190, 1276, 1416
BspACI CCGC 3 cut(s) 322, 1298, 1327
BspANI GGCC 1 cut(s) 1501
BspCNI CTCAG 1 cut(s) 781
BspFNI CGCG 2 cut(s) 1327, 1357
BspHI TCATGA 1 cut(s) 1338
BspLI GGNNCC 3 cut(s) 326, 748, 1500
BspPI GGATC 2 cut(s) 907, 1198
BspT107I GGYRCC 1 cut(s) 324
BspTI CTTAAG 2 cut(s) 290, 454
BspTNI GGTCTC 1 cut(s) 582
BsrDI GCAATG 1 cut(s) 63
BsrGI TGTACA 1 cut(s) 1263
BsrI ACTGG 4 cut(s) 605, 750, 1192, 1580
BssECI CCNNGG 2 cut(s) 1165, 1405
BssMI GATC 6 cut(s) 408, 899, 1075, 1190, 1276, 1416
BssNAI GTATAC 2 cut(s) 355, 1553
BssT1I CCWWGG 1 cut(s) 1405
Bst1107I GTATAC 2 cut(s) 355, 1553
BstAFI CTTAAG 2 cut(s) 290, 454
BstAUI TGTACA 1 cut(s) 1263
BstBAI YACGTR 1 cut(s) 568
BstC8I GCNNGC 2 cut(s) 7, 110
BstDEI CTNAG 6 cut(s) 737, 768, 882, 1064, 1435, 1485
BstEII GGTNACC 2 cut(s) 1400, 1451
BstF5I GGATG 9 cut(s) 92, 284, 328, 491, 615, 936, 1057, 1419, 1427
BstFNI CGCG 2 cut(s) 1327, 1357
BstH2I RGCGCY 1 cut(s) 543
BstHHI GCGC 2 cut(s) 542, 1359
BstKTI GATC 6 cut(s) 411, 902, 1078, 1193, 1279, 1419
BstMAI GTCTC 1 cut(s) 582
BstMBI GATC 6 cut(s) 408, 899, 1075, 1190, 1276, 1416
BstMWI GCNNNNNNNGC 6 cut(s) 539, 610, 662, 674, 923, 1333
BstNSI RCATGY 3 cut(s) 112, 1132, 1240
BstPI GGTNACC 2 cut(s) 1400, 1451
BstSCI CCNGG 4 cut(s) 144, 312, 494, 1165
BstSFI CTRYAG 1 cut(s) 1492
BstUI CGCG 2 cut(s) 1327, 1357
BstV1I GCAGC 6 cut(s) 40, 331, 652, 722, 899, 938
BstX2I RGATCY 2 cut(s) 899, 1190
BstYI RGATCY 2 cut(s) 899, 1190
BstZ17I GTATAC 2 cut(s) 355, 1553
Bsu36I CCTNAGG 1 cut(s) 1485
BsuI GTATCC 1 cut(s) 431
BsuRI GGCC 1 cut(s) 1501
BtsCI GGATG 9 cut(s) 92, 284, 328, 491, 615, 936, 1057, 1419, 1427
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 4 cut(s) 244, 354, 1185, 1259
Cac8I GCNNGC 2 cut(s) 7, 110
CciI TCATGA 1 cut(s) 1338
CfoI GCGC 2 cut(s) 542, 1359
Cfr13I GGNCC 1 cut(s) 1499
CseI GACGC 1 cut(s) 1456
Csp6I GTAC 7 cut(s) 325, 341, 386, 569, 871, 1264, 1568
CviQI GTAC 7 cut(s) 325, 341, 386, 569, 871, 1264, 1568
DdeI CTNAG 6 cut(s) 737, 768, 882, 1064, 1435, 1485
DpnI GATC 6 cut(s) 410, 901, 1077, 1192, 1278, 1418
DpnII GATC 6 cut(s) 408, 899, 1075, 1190, 1276, 1416
Ecl136II GAGCTC 2 cut(s) 613, 677
Eco130I CCWWGG 1 cut(s) 1405
Eco24I GRGCYC 3 cut(s) 615, 679, 1338
Eco31I GGTCTC 1 cut(s) 582
Eco47III AGCGCT 1 cut(s) 541
Eco53kI GAGCTC 2 cut(s) 613, 677
Eco57I CTGAAG 3 cut(s) 972, 1334, 1461
Eco81I CCTNAGG 1 cut(s) 1485
Eco88I CYCGRG 1 cut(s) 334
Eco91I GGTNACC 2 cut(s) 1400, 1451
EcoICRI GAGCTC 2 cut(s) 613, 677
EcoO65I GGTNACC 2 cut(s) 1400, 1451
EcoRI GAATTC 1 cut(s) 721
EcoT14I CCWWGG 1 cut(s) 1405
EcoT38I GRGCYC 3 cut(s) 615, 679, 1338
ErhI CCWWGG 1 cut(s) 1405
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 1 cut(s) 1355
FbaI TGATCA 1 cut(s) 1416
FblI GTMKAC 2 cut(s) 354, 1552
Fnu4HI GCNGC 6 cut(s) 54, 320, 666, 711, 888, 927
FokI GGATG 9 cut(s) 99, 271, 315, 478, 602, 923, 1064, 1414, 1426
FriOI GRGCYC 3 cut(s) 615, 679, 1338
Fsp4HI GCNGC 6 cut(s) 54, 320, 666, 711, 888, 927
FspBI CTAG 4 cut(s) 473, 572, 1301, 1556
GlaI GCGC 2 cut(s) 541, 1358
GluI GCNGC 6 cut(s) 54, 320, 666, 711, 888, 927
GsuI CTGGAG 1 cut(s) 951
HaeII RGCGCY 1 cut(s) 543
HaeIII GGCC 1 cut(s) 1501
HapII CCGG 5 cut(s) 146, 313, 495, 1167, 1502
HgaI GACGC 1 cut(s) 1456
HhaI GCGC 2 cut(s) 542, 1359
Hin6I GCGC 2 cut(s) 540, 1357
HinP1I GCGC 2 cut(s) 540, 1357
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 5 cut(s) 220, 1060, 1170, 1322, 1348
HpaII CCGG 5 cut(s) 146, 313, 495, 1167, 1502
HphI GGTGA 2 cut(s) 1412, 1450
Hpy166II GTNNAC 4 cut(s) 136, 355, 1222, 1553
Hpy188I TCNGA 3 cut(s) 895, 1180, 1286
Hpy188III TCNNGA 3 cut(s) 672, 1312, 1339
Hpy8I GTNNAC 4 cut(s) 136, 355, 1222, 1553
HpyAV CCTTC 2 cut(s) 890, 1441
HpyCH4IV ACGT 1 cut(s) 567
HpyF10VI GCNNNNNNNGC 6 cut(s) 539, 610, 662, 674, 923, 1333
HpyF3I CTNAG 6 cut(s) 737, 768, 882, 1064, 1435, 1485
HpySE526I ACGT 1 cut(s) 567
HspAI GCGC 2 cut(s) 540, 1357
KpnI GGTACC 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 1416
Kzo9I GATC 6 cut(s) 408, 899, 1075, 1190, 1276, 1416
LmnI GCTCC 3 cut(s) 674, 746, 1504
Lsp1109I GCAGC 6 cut(s) 40, 331, 652, 722, 899, 938
LweI GCATC 4 cut(s) 77, 550, 677, 945
MaeI CTAG 4 cut(s) 473, 572, 1301, 1556
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 4 cut(s) 148, 256, 1400, 1451
MalI GATC 6 cut(s) 410, 901, 1077, 1192, 1278, 1418
MboI GATC 6 cut(s) 408, 899, 1075, 1190, 1276, 1416
MboII GAAGA 4 cut(s) 418, 802, 1299, 1517
MfeI CAATTG 2 cut(s) 117, 861
MflI RGATCY 2 cut(s) 899, 1190
MhlI GDGCHC 3 cut(s) 615, 679, 1338
MmeI TCCRAC 2 cut(s) 565, 1299
MseI TTAA 7 cut(s) 275, 291, 374, 455, 867, 1136, 1442
MslI CAYNNNNRTG 2 cut(s) 1239, 1343
MspA1I CMGCKG 3 cut(s) 322, 917, 929
MspCI CTTAAG 2 cut(s) 290, 454
MspI CCGG 5 cut(s) 146, 313, 495, 1167, 1502
MspR9I CCNGG 4 cut(s) 146, 314, 496, 1167
MunI CAATTG 2 cut(s) 117, 861
Mva1269I GAATGC 2 cut(s) 825, 1003
MvnI CGCG 2 cut(s) 1327, 1357
MwoI GCNNNNNNNGC 6 cut(s) 539, 610, 662, 674, 923, 1333
NciI CCSGG 4 cut(s) 146, 314, 496, 1167
NdeII GATC 6 cut(s) 408, 899, 1075, 1190, 1276, 1416
NlaIV GGNNCC 3 cut(s) 326, 748, 1500
NmuCI GTSAC 1 cut(s) 1400
NspI RCATGY 3 cut(s) 112, 1132, 1240
PaeI GCATGC 1 cut(s) 112
PaeR7I CTCGAG 1 cut(s) 334
PagI TCATGA 1 cut(s) 1338
PciI ACATGT 2 cut(s) 1128, 1236
PctI GAATGC 2 cut(s) 825, 1003
PfeI GAWTC 5 cut(s) 220, 1060, 1170, 1322, 1348
PfoI TCCNGGA 1 cut(s) 494
PkrI GCNGC 6 cut(s) 55, 321, 667, 712, 889, 928
Ppu21I YACGTR 1 cut(s) 568
PscI ACATGT 2 cut(s) 1128, 1236
Psp124BI GAGCTC 2 cut(s) 615, 679
PspEI GGTNACC 2 cut(s) 1400, 1451
PspN4I GGNNCC 3 cut(s) 326, 748, 1500
PspPI GGNCC 1 cut(s) 1499
PspXI VCTCGAGB 1 cut(s) 334
PsuI RGATCY 2 cut(s) 899, 1190
PvuII CAGCTG 2 cut(s) 917, 929
RsaI GTAC 7 cut(s) 326, 342, 387, 570, 872, 1265, 1569
RsaNI GTAC 7 cut(s) 325, 341, 386, 569, 871, 1264, 1568
RseI CAYNNNNRTG 2 cut(s) 1239, 1343
SacI GAGCTC 2 cut(s) 615, 679
SaqAI TTAA 7 cut(s) 275, 291, 374, 455, 867, 1136, 1442
SatI GCNGC 6 cut(s) 54, 320, 666, 711, 888, 927
Sau3AI GATC 6 cut(s) 408, 899, 1075, 1190, 1276, 1416
Sau96I GGNCC 1 cut(s) 1499
ScaI AGTACT 1 cut(s) 872
ScrFI CCNGG 4 cut(s) 146, 314, 496, 1167
SduI GDGCHC 3 cut(s) 615, 679, 1338
SfaNI GCATC 4 cut(s) 77, 550, 677, 945
SfcI CTRYAG 1 cut(s) 1492
Sfr274I CTCGAG 1 cut(s) 334
SlaI CTCGAG 1 cut(s) 334
SmiMI CAYNNNNRTG 2 cut(s) 1239, 1343
SmlI CTYRAG 4 cut(s) 38, 290, 334, 454
SmoI CTYRAG 4 cut(s) 38, 290, 334, 454
SphI GCATGC 1 cut(s) 112
SsiI CCGC 3 cut(s) 322, 1298, 1327
SspMI CTAG 4 cut(s) 473, 572, 1301, 1556
SstI GAGCTC 2 cut(s) 615, 679
StyD4I CCNGG 4 cut(s) 144, 312, 494, 1165
StyI CCWWGG 1 cut(s) 1405
TaiI ACGT 1 cut(s) 570
TaqI TCGA 3 cut(s) 335, 813, 1078
TatI WGTACW 3 cut(s) 385, 870, 1263
TfiI GAWTC 5 cut(s) 220, 1060, 1170, 1322, 1348
Tru1I TTAA 7 cut(s) 275, 291, 374, 455, 867, 1136, 1442
Tru9I TTAA 7 cut(s) 275, 291, 374, 455, 867, 1136, 1442
TscAI CASTG 4 cut(s) 244, 354, 1192, 1266
TseFI GTSAC 1 cut(s) 1400
TseI GCWGC 6 cut(s) 53, 319, 665, 710, 887, 926
Tsp45I GTSAC 1 cut(s) 1400
TspDTI ATGAA 6 cut(s) 87, 185, 508, 589, 795, 996
TspRI CASTG 4 cut(s) 244, 354, 1192, 1266
Vha464I CTTAAG 2 cut(s) 290, 454
XapI RAATTY 4 cut(s) 525, 721, 779, 1307
XceI RCATGY 3 cut(s) 112, 1132, 1240
XhoI CTCGAG 1 cut(s) 334
XmiI GTMKAC 2 cut(s) 354, 1552
XspI CTAG 4 cut(s) 473, 572, 1301, 1556
ZrmI AGTACT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.