pycom09g04080

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0008949
Physical Location & Seq
Reverse (-)
3010811 .. 3022495
11685 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 837 bp
ATGGCATACATGCACATTTCATGGCTTCTTCTAATTCAATGCCTACTTCTGGTCACCAGAATTGGTGCCAAAGTTCCAGCAATCATTGTGTTTGGAGACTCATCGGTCGATGCTGGGAACAACAACCAGATTCCCACAATTGCCAGGAGCAACTTTCAGCCTTATGGCCGTGACTTCGCCAGTGGCAAGCCTACTGGGAGATTCTCTAATGGCAGAGTTCCCACGGACTTCATCTCCGAAGCTTTTGGACTCAAACCATTCGTACCTGCCTACTTGGATCCTACTTATAACATATCAGATTTTGCAACTGGAGTCACCTTTGCTTCTGCTGGCACTGGCTACGACACTGCAACTTCAGATGTTCTATCTGTGATTCCACTATGGAAGCAAGTGCAGTACTACAAGGAATACCAGAGCAAACTGAGACTTCATCTAGGAGGAAACAAAACAAAGGAAACAATCCATGAAGCTTTGCACATGACAAGCTTAGGAACCAATGATTTCCTTGAAAACTACTACTCATACCCCGGCCGGTCGAATCAATACTCTATCGAACAATACCAAGATTTTCTCATTGGAATTGCAGGAAATTTCATCAAACAACTCTACGGTCTTGGAGCTCGGAAAATATCCTTAGGAGGCCTACCTCCGATGGGGTGCTTGCCATTGGAGAGAACCACCAATGTGATGGGTGGAAATGACTGCATTTCAAGTTACAACAATGTGGCCTTGGAGTTCAATGGCAAGTTGAAGACCTTGACTACAAGCCTTAACAAAGAGCTTACTGGGATCAAATTGTGGTTTTTTCAAACCCATATTACATTTTCCTGCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

279

Amino Acids

30.77

Weight (kDa)

8.55

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 261 3.9e-20 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AasI GACNNNNNNGTC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 274
AccB1I GGYRCC 1 cut(s) 65
AclWI GGATC 3 cut(s) 272, 285, 797
AcoI YGGCCR 2 cut(s) 166, 529
AcsI RAATTY 1 cut(s) 589
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 2 cut(s) 264, 398
AfiI CCNNNNNNNGG 2 cut(s) 49, 653
AgsI TTSAA 6 cut(s) 38, 509, 711, 739, 751, 809
AjnI CCWGG 1 cut(s) 143
AleI CACNNNNGTG 1 cut(s) 683
AluBI AGCT 5 cut(s) 242, 470, 486, 620, 781
AluI AGCT 5 cut(s) 242, 470, 486, 620, 781
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 2 cut(s) 90, 418
AlwI GGATC 3 cut(s) 272, 285, 797
AoxI GGCC 4 cut(s) 166, 529, 640, 726
ApoI RAATTY 1 cut(s) 589
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 2 cut(s) 46, 307
AxyI CCTNAGG 1 cut(s) 634
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 758
Bbv12I GWGCWC 1 cut(s) 622
BccI CCATC 2 cut(s) 646, 682
BceAI ACGGC 1 cut(s) 153
BciT130I CCWGG 1 cut(s) 145
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 2 cut(s) 90, 418
BfaI CTAG 1 cut(s) 434
BfuAI ACCTGC 1 cut(s) 274
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 2 cut(s) 145, 528
BmiI GGNNCC 3 cut(s) 67, 279, 493
BmrFI CCNGG 2 cut(s) 145, 528
BmrI ACTGGG 2 cut(s) 204, 795
BmsI GCATC 1 cut(s) 100
BmuI ACTGGG 2 cut(s) 204, 795
BpiI GAAGAC 1 cut(s) 758
BpmI CTGGAG 1 cut(s) 330
Bpu10I CCTNAGC 1 cut(s) 487
BpuMI CCSGG 1 cut(s) 528
BsaJI CCNNGG 3 cut(s) 222, 526, 729
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 653
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 5 cut(s) 180, 199, 313, 340, 790
Bse21I CCTNAGG 1 cut(s) 634
BseBI CCWGG 1 cut(s) 145
BseDI CCNNGG 3 cut(s) 222, 526, 729
BseLI CCNNNNNNNGG 2 cut(s) 49, 653
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 5 cut(s) 180, 199, 313, 340, 790
BseX3I CGGCCG 1 cut(s) 529
BseYI CCCAGC 1 cut(s) 113
BsgI GTGCAG 2 cut(s) 413, 814
Bsh1285I CGRYCG 3 cut(s) 108, 532, 536
BshFI GGCC 4 cut(s) 168, 531, 642, 728
BshNI GGYRCC 1 cut(s) 65
BsiEI CGRYCG 3 cut(s) 108, 532, 536
BsiHKAI GWGCWC 1 cut(s) 622
BsiSI CCGG 2 cut(s) 528, 532
BslI CCNNNNNNNGG 2 cut(s) 49, 653
BsmAI GTCTC 2 cut(s) 90, 418
BsnI GGCC 4 cut(s) 168, 531, 642, 728
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 2 cut(s) 277, 789
BspANI GGCC 4 cut(s) 168, 531, 642, 728
BspCNI CTCAG 1 cut(s) 414
BspLI GGNNCC 3 cut(s) 67, 279, 493
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 3 cut(s) 272, 285, 797
BspT107I GGYRCC 1 cut(s) 65
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 5 cut(s) 180, 199, 313, 340, 790
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 3 cut(s) 222, 526, 729
BssMI GATC 2 cut(s) 277, 789
BssT1I CCWWGG 1 cut(s) 729
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 1 cut(s) 611
BstC8I GCNNGC 3 cut(s) 188, 331, 662
BstDEI CTNAG 3 cut(s) 422, 487, 634
BstDSI CCRYGG 1 cut(s) 222
BstEII GGTNACC 1 cut(s) 52
BstKTI GATC 2 cut(s) 280, 792
BstMAI GTCTC 2 cut(s) 90, 418
BstMBI GATC 2 cut(s) 277, 789
BstMCI CGRYCG 3 cut(s) 108, 532, 536
BstNI CCWGG 1 cut(s) 145
BstNSI RCATGY 1 cut(s) 13
BstPI GGTNACC 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 143, 526
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 1 cut(s) 277
BstXI CCANNNNNNTGG 1 cut(s) 688
BstYI RGATCY 1 cut(s) 277
BstZI CGGCCG 1 cut(s) 529
Bsu36I CCTNAGG 1 cut(s) 634
BsuRI GGCC 4 cut(s) 168, 531, 642, 728
BtgI CCRYGG 1 cut(s) 222
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 3 cut(s) 187, 333, 345
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 3 cut(s) 188, 331, 662
Cfr10I RCCGGY 1 cut(s) 531
Csp6I GTAC 2 cut(s) 263, 397
CviAII CATG 5 cut(s) 10, 21, 464, 478, 834
CviQI GTAC 2 cut(s) 263, 397
DdeI CTNAG 3 cut(s) 422, 487, 634
DpnI GATC 2 cut(s) 279, 791
DpnII GATC 2 cut(s) 277, 789
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 2 cut(s) 166, 529
EagI CGGCCG 1 cut(s) 529
Ecl136II GAGCTC 1 cut(s) 620
EclXI CGGCCG 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 729
Eco147I AGGCCT 1 cut(s) 642
Eco24I GRGCYC 1 cut(s) 622
Eco52I CGGCCG 1 cut(s) 529
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 1 cut(s) 339
Eco81I CCTNAGG 1 cut(s) 634
Eco91I GGTNACC 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 620
EcoO65I GGTNACC 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 729
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 729
FaeI CATG 5 cut(s) 13, 24, 467, 481, 837
FatI CATG 5 cut(s) 9, 20, 463, 477, 833
FriOI GRGCYC 1 cut(s) 622
FspBI CTAG 1 cut(s) 434
GsaI CCCAGC 1 cut(s) 117
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 4 cut(s) 168, 531, 642, 728
HapII CCGG 2 cut(s) 528, 532
Hin1II CATG 5 cut(s) 13, 24, 467, 481, 837
HindIII AAGCTT 3 cut(s) 240, 468, 484
HinfI GANTC 7 cut(s) 98, 130, 201, 249, 312, 373, 538
HpaII CCGG 2 cut(s) 528, 532
HphI GGTGA 2 cut(s) 46, 307
Hpy188I TCNGA 5 cut(s) 238, 298, 358, 624, 651
HpyCH4III ACNGT 1 cut(s) 611
HpyCH4V TGCA 8 cut(s) 13, 305, 350, 394, 475, 584, 705, 831
HpyF3I CTNAG 3 cut(s) 422, 487, 634
Hsp92II CATG 5 cut(s) 13, 24, 467, 481, 837
Kzo9I GATC 2 cut(s) 277, 789
LmnI GCTCC 2 cut(s) 147, 617
LweI GCATC 1 cut(s) 100
MaeI CTAG 1 cut(s) 434
MaeIII GTNAC 4 cut(s) 52, 170, 313, 713
MalI GATC 2 cut(s) 279, 791
MboI GATC 2 cut(s) 277, 789
MboII GAAGA 2 cut(s) 20, 763
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 6 cut(s) 33, 60, 138, 579, 589, 794
MlyI GAGTC 3 cut(s) 92, 243, 321
MnlI CCTC 3 cut(s) 431, 632, 657
MseI TTAA 1 cut(s) 771
MslI CAYNNNNRTG 1 cut(s) 683
MspI CCGG 2 cut(s) 528, 532
MspR9I CCNGG 2 cut(s) 145, 528
MunI CAATTG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 145
NciI CCSGG 1 cut(s) 528
NdeII GATC 2 cut(s) 277, 789
NlaIII CATG 5 cut(s) 13, 24, 467, 481, 837
NlaIV GGNNCC 3 cut(s) 67, 279, 493
NmuCI GTSAC 3 cut(s) 52, 170, 313
NspI RCATGY 1 cut(s) 13
OliI CACNNNNGTG 1 cut(s) 683
PceI AGGCCT 1 cut(s) 642
PfeI GAWTC 4 cut(s) 130, 201, 373, 538
PleI GAGTC 3 cut(s) 92, 243, 320
PpsI GAGTC 3 cut(s) 92, 243, 320
PsiI TTATAA 1 cut(s) 288
Psp124BI GAGCTC 1 cut(s) 622
Psp6I CCWGG 1 cut(s) 143
PspEI GGTNACC 1 cut(s) 52
PspFI CCCAGC 1 cut(s) 113
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 3 cut(s) 67, 279, 493
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 2 cut(s) 264, 398
RsaNI GTAC 2 cut(s) 263, 397
RseI CAYNNNNRTG 1 cut(s) 683
SacI GAGCTC 1 cut(s) 622
SaqAI TTAA 1 cut(s) 771
Sau3AI GATC 2 cut(s) 277, 789
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 3 cut(s) 92, 243, 321
ScrFI CCNGG 2 cut(s) 145, 528
SduI GDGCHC 1 cut(s) 622
SetI ASST 9 cut(s) 244, 268, 320, 472, 488, 622, 649, 758, 783
SfaNI GCATC 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 683
Sse9I AATT 6 cut(s) 33, 60, 138, 579, 589, 794
SseBI AGGCCT 1 cut(s) 642
SspMI CTAG 1 cut(s) 434
SstI GAGCTC 1 cut(s) 622
StuI AGGCCT 1 cut(s) 642
StyD4I CCNGG 2 cut(s) 143, 526
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 1 cut(s) 611
TaqI TCGA 3 cut(s) 108, 536, 552
TaqII GACCGA 1 cut(s) 94
TasI AATT 6 cut(s) 33, 60, 138, 579, 589, 794
TatI WGTACW 1 cut(s) 396
TfiI GAWTC 4 cut(s) 130, 201, 373, 538
Tru1I TTAA 1 cut(s) 771
Tru9I TTAA 1 cut(s) 771
TscAI CASTG 3 cut(s) 187, 340, 352
TseFI GTSAC 3 cut(s) 52, 170, 313
Tsp45I GTSAC 3 cut(s) 52, 170, 313
TspDTI ATGAA 5 cut(s) 9, 220, 419, 480, 583
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 3 cut(s) 187, 340, 352
XapI RAATTY 1 cut(s) 589
XceI RCATGY 1 cut(s) 13
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XspI CTAG 1 cut(s) 434
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.