pycom09g09550
ERF Family

2OG-Fe(II) oxygenase superfamily

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
Physical Location & Seq
Reverse (-)
7723384 .. 7726124
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g09550.1

Sequence Viewer

Length: 1494 bp
ATGGACAATCGCGCTCAAGATGGCGACATCTCCCGCGGCGGACGATTCTCAACGAAACGGGGAGGTCAAGCTCGAGAAAGCCTCCGAGGCGGACGATTCTCCACGAACCGGGGAGGTCAAGCTCGAGAAAGCCTCCGAGGCGGACGTTTTCCCACGAATCGGGGAGGTCCAGCTCGAGGAAACCAACGGGGTGGCCGACAGTCTCCTCTCGCCGCCGGCCACCCTTCCAATCTCCACAGCCCTCAGGCCAGGCCTGGAACTCGTGGAACATTTTCTAATGACTCCTCGGGTAACAATGAGTTTCGGGTATCATATCATTCTGAGCATAAACCTGCCACAAGTTCTGCACATCGGCAGGAATGGCGTTCATCTGGAAGAGAGGACACGCATTCTCATGCTGAGAGTTTGAAGGAGGGACATAAATCAACTGAGTCCAGGGGTGTTAACCCGAAAGTTTCAAAGTGGTCAGTGGTCTCAGATTCAAACTACAGAGAGCATCCTCCTTCGTTTTCTAATCTGAGTCATTCTGAGAATTTGCTTGCCGGGGTAGAGAGAATGCAGATTAGAGAAACCCCAAGTGAGATGACAGAGGATAGTGTAACTCTGCCCTGCAAATCTGAGGCCCATGGTGTGCCTTTCCCTATTAACAGTCCTGTAAATGCAACCAACCAAGGGAAGCCAGAGCCTTCTGAACAGTCGACTTTTGATTTATGTCCTCCTAAATCAAGGGGTTCTATTACCCTAAAGGCTCCTCTGCTAGCACAGAATAGAGAAAGGCGAAATGAAATCAAGCGCTCCAAGGAACAGAACAGTGGAATCATTTTGCAGTCTGGAATGGTTCTATTAAAAAGTTACCTTTCCCTCCATGACCAGATGAATATAGTCAAGTTATGCCGAGACTTGGGTTTGGGTTCTGGAGGTTTCTACAATCCTGGTTACCGTGATGGAGCAAAGCTGAATTTGATGATGATGTGCCTTGGTAAGAATTGGGACCCTGAGACAAGTCAGTATGGAGATCATCGACCTGTTGATGGTGCTATTCCGCCAAAGATTCCTGATGAATTCTTTCAGTTGGTTGAAAGTGCAATCAAAGAGTCCCAGTCCCTTATTATGAAAGATTCTAAAACTAGCAATTCTCAAGGCATACTTCCATGGATGATCCCAGACATATGTCTTGTGAACTTTTACTCATCCTCTGGGCGACTAGGTCTTCATCAGGATCGTGATGAAAGTGAACAAAGTCTCCGCAAATGTTTGCCAGTTGTCTCTTTTTCCATTGGAGATACTGCAGAATTCTTGTACGGTGAGCAAAGGGATGTTGACAGGGCAAATAAGGTTATACTGGAATCTGGAGATGTTCTAATATTTGGTGGAAAATCGAGGCATGTATTTCATGGTGTATCGTCTATAAAGCCAGACACTGCTCCTAAGACCCTGCTGGAAAAAACAAATCTCCGAGCTGGCCGTCTGAATCTTACTTTCAGAGAGTATTGA

Protein Analysis

498

Amino Acids

54.92

Weight (kDa)

9.37

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 278 - 495 1.8e-45 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 340
AccI GTMKAC 1 cut(s) 698
AccII CGCG 2 cut(s) 12, 36
AciI CCGC 8 cut(s) 34, 36, 39, 90, 141, 213, 1043, 1246
AclWI GGATC 2 cut(s) 1153, 1227
AcoI YGGCCR 3 cut(s) 193, 217, 1462
AcsI RAATTY 4 cut(s) 532, 958, 1061, 1292
AfaI GTAC 1 cut(s) 1301
AfeI AGCGCT 1 cut(s) 794
AfiI CCNNNNNNNGG 6 cut(s) 108, 159, 176, 672, 901, 1031
AgsI TTSAA 4 cut(s) 409, 459, 483, 1079
AjnI CCWGG 4 cut(s) 248, 253, 434, 931
AloI GAACNNNNNNTCC 2 cut(s) 1227, 1259
AluBI AGCT 5 cut(s) 71, 122, 173, 955, 1460
AluI AGCT 5 cut(s) 71, 122, 173, 955, 1460
Alw26I GTCTC 6 cut(s) 207, 478, 891, 992, 1247, 1270
AlwI GGATC 2 cut(s) 1153, 1227
AlwNI CAGNNNCTG 1 cut(s) 1421
Ama87I CYCGRG 4 cut(s) 72, 123, 174, 286
Aor51HI AGCGCT 1 cut(s) 794
AoxI GGCC 6 cut(s) 193, 217, 246, 251, 621, 1462
ApoI RAATTY 4 cut(s) 532, 958, 1061, 1292
Asp700I GAANNNNTTC 2 cut(s) 271, 1065
AspLEI GCGC 2 cut(s) 14, 795
AspS9I GGNCC 3 cut(s) 167, 622, 991
AsuC2I CCSGG 2 cut(s) 110, 544
AsuHPI GGTGA 1 cut(s) 1316
AsuNHI GCTAGC 1 cut(s) 757
AvaI CYCGRG 4 cut(s) 72, 123, 174, 286
AvaII GGWCC 2 cut(s) 167, 991
AxyI CCTNAGG 1 cut(s) 243
BauI CACGAG 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 1202
BccI CCATC 3 cut(s) 14, 938, 1025
BceAI ACGGC 1 cut(s) 1449
BciT130I CCWGG 4 cut(s) 250, 255, 436, 933
BcnI CCSGG 2 cut(s) 110, 544
BcoDI GTCTC 6 cut(s) 207, 478, 891, 992, 1247, 1270
BfaI CTAG 3 cut(s) 758, 1128, 1205
BfmI CTRYAG 2 cut(s) 487, 1287
BfoI RGCGCY 1 cut(s) 796
BfuAI ACCTGC 1 cut(s) 340
BglI GCCNNNNNGGC 2 cut(s) 87, 138
BisI GCNGC 2 cut(s) 37, 213
BlsI GCNGC 2 cut(s) 38, 214
Bme1390I CCNGG 6 cut(s) 110, 250, 255, 436, 544, 933
Bme18I GGWCC 2 cut(s) 167, 991
BmeT110I CYCGRG 4 cut(s) 72, 123, 174, 286
BmgT120I GGNCC 3 cut(s) 167, 622, 991
BmiI GGNNCC 3 cut(s) 750, 992, 993
BmrFI CCNGG 6 cut(s) 110, 250, 255, 436, 544, 933
BmrI ACTGGG 1 cut(s) 1093
BmsI GCATC 1 cut(s) 505
BmtI GCTAGC 1 cut(s) 761
BmuI ACTGGG 1 cut(s) 1093
BoxI GACNNNNGTC 1 cut(s) 1170
BpiI GAAGAC 1 cut(s) 1202
BplI GAGNNNNNCTC 4 cut(s) 66, 98, 117, 149
BpmI CTGGAG 2 cut(s) 936, 1371
BpuEI CTTGAG 1 cut(s) 1122
BpuMI CCSGG 2 cut(s) 110, 544
BsaI GGTCTC 1 cut(s) 478
BsaXI ACNNNNNCTCC 2 cut(s) 1227, 1257
Bsc4I CCNNNNNNNGG 6 cut(s) 108, 159, 176, 672, 901, 1031
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 3 cut(s) 1099, 1259, 1347
Bse21I CCTNAGG 1 cut(s) 243
BseBI CCWGG 4 cut(s) 250, 255, 436, 933
BseGI GGATG 4 cut(s) 496, 1161, 1190, 1321
BseLI CCNNNNNNNGG 6 cut(s) 108, 159, 176, 672, 901, 1031
BseMII CTCAG 9 cut(s) 257, 312, 390, 420, 489, 509, 519, 609, 987
BseNI ACTGG 3 cut(s) 1099, 1259, 1347
BseRI GAGGAG 3 cut(s) 195, 274, 741
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 2 cut(s) 12, 36
BshFI GGCC 6 cut(s) 195, 219, 248, 253, 623, 1464
BsiHKCI CYCGRG 4 cut(s) 72, 123, 174, 286
BsiSI CCGG 3 cut(s) 109, 216, 543
BslFI GGGAC 4 cut(s) 429, 1004, 1081, 1087
BslI CCNNNNNNNGG 6 cut(s) 108, 159, 176, 672, 901, 1031
BsmAI GTCTC 6 cut(s) 207, 478, 891, 992, 1247, 1270
BsmFI GGGAC 4 cut(s) 429, 1004, 1081, 1087
BsmI GAATGC 2 cut(s) 388, 561
BsnI GGCC 6 cut(s) 195, 219, 248, 253, 623, 1464
Bso31I GGTCTC 1 cut(s) 478
BsoBI CYCGRG 4 cut(s) 72, 123, 174, 286
Bsp143I GATC 3 cut(s) 1015, 1158, 1219
Bsp19I CCATGG 2 cut(s) 625, 1151
BspACI CCGC 8 cut(s) 34, 36, 39, 90, 141, 213, 1043, 1246
BspANI GGCC 6 cut(s) 195, 219, 248, 253, 623, 1464
BspCNI CTCAG 9 cut(s) 256, 313, 391, 421, 488, 510, 520, 610, 988
BspFNI CGCG 2 cut(s) 12, 36
BspLI GGNNCC 3 cut(s) 750, 992, 993
BspMAI CTGCAG 1 cut(s) 1291
BspMI ACCTGC 1 cut(s) 340
BspOI GCTAGC 1 cut(s) 761
BspPI GGATC 2 cut(s) 1153, 1227
BspTNI GGTCTC 1 cut(s) 478
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 3 cut(s) 1099, 1259, 1347
BssAI RCCGGY 1 cut(s) 215
BssMI GATC 3 cut(s) 1015, 1158, 1219
BssSI CACGAG 1 cut(s) 261
BssT1I CCWWGG 5 cut(s) 625, 670, 798, 976, 1151
Bst2BI CACGAG 1 cut(s) 261
Bst2UI CCWGG 4 cut(s) 250, 255, 436, 933
Bst4CI ACNGT 6 cut(s) 201, 650, 696, 812, 941, 1304
Bst6I CTCTTC 1 cut(s) 370
BstC8I GCNNGC 4 cut(s) 217, 540, 759, 1462
BstDSI CCRYGG 3 cut(s) 34, 625, 1151
BstEII GGTNACC 1 cut(s) 935
BstF5I GGATG 4 cut(s) 496, 1161, 1190, 1321
BstFNI CGCG 2 cut(s) 12, 36
BstH2I RGCGCY 1 cut(s) 796
BstHHI GCGC 2 cut(s) 14, 795
BstKTI GATC 3 cut(s) 1018, 1161, 1222
BstMAI GTCTC 6 cut(s) 207, 478, 891, 992, 1247, 1270
BstMBI GATC 3 cut(s) 1015, 1158, 1219
BstMWI GCNNNNNNNGC 3 cut(s) 87, 138, 361
BstNI CCWGG 4 cut(s) 250, 255, 436, 933
BstNSI RCATGY 1 cut(s) 1388
BstPAI GACNNNNGTC 1 cut(s) 1170
BstPI GGTNACC 1 cut(s) 935
BstSCI CCNGG 6 cut(s) 108, 248, 253, 434, 542, 931
BstSFI CTRYAG 2 cut(s) 487, 1287
BstUI CGCG 2 cut(s) 12, 36
BstV2I GAAGAC 1 cut(s) 1202
BstXI CCANNNNNNTGG 1 cut(s) 191
Bsu36I CCTNAGG 1 cut(s) 243
BsuRI GGCC 6 cut(s) 195, 219, 248, 253, 623, 1464
BtgI CCRYGG 3 cut(s) 34, 625, 1151
BtsCI GGATG 4 cut(s) 496, 1161, 1190, 1321
BtsI GCAGTG 1 cut(s) 1419
BtsIMutI CAGTG 3 cut(s) 474, 817, 1419
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 4 cut(s) 217, 540, 759, 1462
CaiI CAGNNNCTG 1 cut(s) 1421
CfoI GCGC 2 cut(s) 14, 795
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 3 cut(s) 167, 622, 991
Cfr42I CCGCGG 1 cut(s) 37
Csp6I GTAC 1 cut(s) 1300
CviAII CATG 6 cut(s) 395, 626, 866, 1152, 1385, 1394
CviQI GTAC 1 cut(s) 1300
DpnI GATC 3 cut(s) 1017, 1160, 1221
DpnII GATC 3 cut(s) 1015, 1158, 1219
EaeI YGGCCR 3 cut(s) 193, 217, 1462
Eam1104I CTCTTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 370
EciI GGCGGA 4 cut(s) 54, 105, 156, 1032
Eco130I CCWWGG 5 cut(s) 625, 670, 798, 976, 1151
Eco147I AGGCCT 1 cut(s) 253
Eco31I GGTCTC 1 cut(s) 478
Eco47I GGWCC 2 cut(s) 167, 991
Eco47III AGCGCT 1 cut(s) 794
Eco81I CCTNAGG 1 cut(s) 243
Eco88I CYCGRG 4 cut(s) 72, 123, 174, 286
Eco91I GGTNACC 1 cut(s) 935
EcoO109I RGGNCCY 1 cut(s) 991
EcoO65I GGTNACC 1 cut(s) 935
EcoRI GAATTC 2 cut(s) 1061, 1292
EcoRII CCWGG 4 cut(s) 248, 253, 434, 931
EcoT14I CCWWGG 5 cut(s) 625, 670, 798, 976, 1151
ErhI CCWWGG 5 cut(s) 625, 670, 798, 976, 1151
FaeI CATG 6 cut(s) 398, 629, 869, 1155, 1388, 1397
FalI AAGNNNNNCTT 2 cut(s) 1131, 1163
FaqI GGGAC 4 cut(s) 429, 1004, 1081, 1087
FatI CATG 6 cut(s) 394, 625, 865, 1151, 1384, 1393
FauI CCCGC 1 cut(s) 41
FauNDI CATATG 1 cut(s) 1169
FblI GTMKAC 1 cut(s) 698
Fnu4HI GCNGC 2 cut(s) 37, 213
FokI GGATG 4 cut(s) 483, 1168, 1177, 1328
Fsp4HI GCNGC 2 cut(s) 37, 213
FspBI CTAG 3 cut(s) 758, 1128, 1205
GlaI GCGC 2 cut(s) 13, 794
GluI GCNGC 2 cut(s) 37, 213
GsuI CTGGAG 2 cut(s) 936, 1371
HaeII RGCGCY 1 cut(s) 796
HaeIII GGCC 6 cut(s) 195, 219, 248, 253, 623, 1464
HapII CCGG 3 cut(s) 109, 216, 543
HhaI GCGC 2 cut(s) 14, 795
Hin1II CATG 6 cut(s) 398, 629, 869, 1155, 1388, 1397
Hin6I GCGC 2 cut(s) 12, 793
HinP1I GCGC 2 cut(s) 12, 793
HincII GTYRAC 3 cut(s) 445, 699, 1321
HindII GTYRAC 3 cut(s) 445, 699, 1321
HpaI GTTAAC 1 cut(s) 445
HpaII CCGG 3 cut(s) 109, 216, 543
HphI GGTGA 1 cut(s) 1316
Hpy166II GTNNAC 5 cut(s) 445, 699, 1180, 1235, 1321
Hpy8I GTNNAC 5 cut(s) 445, 699, 1180, 1235, 1321
HpyAV CCTTC 4 cut(s) 234, 403, 513, 696
HpyCH4III ACNGT 6 cut(s) 201, 650, 696, 812, 941, 1304
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 7 cut(s) 347, 559, 612, 662, 826, 1085, 1289
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 138, 361
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 6 cut(s) 398, 629, 869, 1155, 1388, 1397
HspAI GCGC 2 cut(s) 12, 793
KflI GGGWCCC 1 cut(s) 991
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
KspAI GTTAAC 1 cut(s) 445
KspI CCGCGG 1 cut(s) 37
Kzo9I GATC 3 cut(s) 1015, 1158, 1219
LmnI GCTCC 4 cut(s) 754, 800, 947, 1429
LweI GCATC 1 cut(s) 505
MaeI CTAG 3 cut(s) 758, 1128, 1205
MaeII ACGT 1 cut(s) 145
MaeIII GTNAC 4 cut(s) 290, 598, 851, 935
MalI GATC 3 cut(s) 1017, 1160, 1221
MboI GATC 3 cut(s) 1015, 1158, 1219
MboII GAAGA 2 cut(s) 387, 1202
MluCI AATT 6 cut(s) 532, 958, 985, 1061, 1132, 1292
MlyI GAGTC 4 cut(s) 275, 440, 529, 1103
MroNI GCCGGC 1 cut(s) 215
MroXI GAANNNNTTC 2 cut(s) 271, 1065
MseI TTAA 3 cut(s) 444, 645, 845
MslI CAYNNNNRTG 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 3 cut(s) 109, 216, 543
MspR9I CCNGG 6 cut(s) 110, 250, 255, 436, 544, 933
Mva1269I GAATGC 2 cut(s) 388, 561
MvaI CCWGG 4 cut(s) 250, 255, 436, 933
MvnI CGCG 2 cut(s) 12, 36
MwoI GCNNNNNNNGC 3 cut(s) 87, 138, 361
NaeI GCCGGC 1 cut(s) 217
NciI CCSGG 2 cut(s) 110, 544
NcoI CCATGG 2 cut(s) 625, 1151
NdeI CATATG 1 cut(s) 1169
NdeII GATC 3 cut(s) 1015, 1158, 1219
NgoMIV GCCGGC 1 cut(s) 215
NheI GCTAGC 1 cut(s) 757
NlaIII CATG 6 cut(s) 398, 629, 869, 1155, 1388, 1397
NlaIV GGNNCC 3 cut(s) 750, 992, 993
NmeAIII GCCGAG 1 cut(s) 920
NspI RCATGY 1 cut(s) 1388
PaeR7I CTCGAG 3 cut(s) 72, 123, 174
PceI AGGCCT 1 cut(s) 253
PcsI WCGNNNNNNNCGW 1 cut(s) 193
PctI GAATGC 2 cut(s) 388, 561
PdiI GCCGGC 1 cut(s) 217
PdmI GAANNNNTTC 2 cut(s) 271, 1065
PfeI GAWTC 9 cut(s) 45, 96, 157, 479, 816, 1051, 1118, 1346, 1471
PflFI GACNNNGTC 1 cut(s) 1206
PkrI GCNGC 2 cut(s) 38, 214
PleI GAGTC 4 cut(s) 275, 439, 528, 1102
PpsI GAGTC 4 cut(s) 275, 439, 528, 1102
PpuMI RGGWCCY 1 cut(s) 991
PshAI GACNNNNGTC 1 cut(s) 1170
Psp5II RGGWCCY 1 cut(s) 991
Psp6I CCWGG 4 cut(s) 248, 253, 434, 931
PspEI GGTNACC 1 cut(s) 935
PspGI CCWGG 4 cut(s) 248, 253, 434, 931
PspN4I GGNNCC 3 cut(s) 750, 992, 993
PspPI GGNCC 3 cut(s) 167, 622, 991
PspPPI RGGWCCY 1 cut(s) 991
PspXI VCTCGAGB 1 cut(s) 174
PstI CTGCAG 1 cut(s) 1291
PstNI CAGNNNCTG 1 cut(s) 1421
PsyI GACNNNGTC 1 cut(s) 1206
RsaI GTAC 1 cut(s) 1301
RsaNI GTAC 1 cut(s) 1300
RseI CAYNNNNRTG 1 cut(s) 393
SacII CCGCGG 1 cut(s) 37
SalI GTCGAC 1 cut(s) 697
SaqAI TTAA 3 cut(s) 444, 645, 845
SatI GCNGC 2 cut(s) 37, 213
Sau3AI GATC 3 cut(s) 1015, 1158, 1219
Sau96I GGNCC 3 cut(s) 167, 622, 991
SchI GAGTC 4 cut(s) 275, 440, 529, 1103
ScrFI CCNGG 6 cut(s) 110, 250, 255, 436, 544, 933
SfaNI GCATC 1 cut(s) 505
SfcI CTRYAG 2 cut(s) 487, 1287
Sfr274I CTCGAG 3 cut(s) 72, 123, 174
Sfr303I CCGCGG 1 cut(s) 37
SgrBI CCGCGG 1 cut(s) 37
SinI GGWCC 2 cut(s) 167, 991
SlaI CTCGAG 3 cut(s) 72, 123, 174
SmiMI CAYNNNNRTG 1 cut(s) 393
SmlI CTYRAG 5 cut(s) 15, 72, 123, 174, 1137
SmoI CTYRAG 5 cut(s) 15, 72, 123, 174, 1137
Sse9I AATT 6 cut(s) 532, 958, 985, 1061, 1132, 1292
SseBI AGGCCT 1 cut(s) 253
SsiI CCGC 8 cut(s) 34, 36, 39, 90, 141, 213, 1043, 1246
SspI AATATT 1 cut(s) 1365
SspMI CTAG 3 cut(s) 758, 1128, 1205
StuI AGGCCT 1 cut(s) 253
StyD4I CCNGG 6 cut(s) 108, 248, 253, 434, 542, 931
StyI CCWWGG 5 cut(s) 625, 670, 798, 976, 1151
TaaI ACNGT 6 cut(s) 201, 650, 696, 812, 941, 1304
TaiI ACGT 1 cut(s) 148
TaqI TCGA 6 cut(s) 73, 124, 175, 698, 1021, 1379
TasI AATT 6 cut(s) 532, 958, 985, 1061, 1132, 1292
TauI GCSGC 2 cut(s) 39, 215
TfiI GAWTC 9 cut(s) 45, 96, 157, 479, 816, 1051, 1118, 1346, 1471
Tru1I TTAA 3 cut(s) 444, 645, 845
Tru9I TTAA 3 cut(s) 444, 645, 845
TscAI CASTG 3 cut(s) 474, 817, 1426
TspDTI ATGAA 8 cut(s) 357, 798, 890, 1074, 1127, 1202, 1242, 1382
TspRI CASTG 3 cut(s) 474, 817, 1426
Tth111I GACNNNGTC 1 cut(s) 1206
VpaK11BI GGWCC 2 cut(s) 167, 991
XapI RAATTY 4 cut(s) 532, 958, 1061, 1292
XceI RCATGY 1 cut(s) 1388
XhoI CTCGAG 3 cut(s) 72, 123, 174
XmiI GTMKAC 1 cut(s) 698
XmnI GAANNNNTTC 2 cut(s) 271, 1065
XspI CTAG 3 cut(s) 758, 1128, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.