Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0007047

Physical Location & Seq

Genomic Coordinates Forward (+)

50575923 .. 50587677

Gene Structure 11755 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0395100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3120 bp

ATGCCGACTTACAGTATCAGAGGCATCGATGTCGATTTCCCATTCGAGGCCTACGATTGCCAGCTCGTTTACATGGAGAAAGTCATTCAGTCTCTCCAACAAAGATCTAACGCACTGTTGGAGAGTCCGACTGGAACTGGGAAAACCCTTTGTCTTCTCTGTGCCACATTGGCTTGGAGGAAGAGCTTGGGGGTTTTCTCAACTGGTCCGAATGTGAAGTTGAGTCAGATCAATGGTGATAAATCAGGTGCTTCATTGTCCCAGTCCCAGTCCCAGTCCCAGACCAAAGATCATCCTACCATAATATATGCGTCCCGCACTCACAGCCAGATCCGGCAAGTAATTCAAGAGTTGAAAAGAAGCTCTTACAGGCCGAAAATGGTAGTATTGGGTTCTCGAGAGCAACTATGCATTCATGATGAAGTGAGTTCACTTCATGGAAAAGCACAGACGAATGCCTGTCACTTCCTCTGTAGAAAGCGTACAAAGCGTTATTGCAAACATTATTCTCGTACTGCTGATTACTTGAAGAATAATTCTCATCTTGGAGATGAACCTATTGACATTGAGGATTTGGTCAATACTGGAAGAAAGTCTGGGCCGTGCCCTTATTACTTATCCCGGGATCTTCATAAGGTTGTTGATATATTGTTTGTACCTTACAACTATCTTATTGATCGCGGGTTTAGAAAATCACTACAAATTAATTGGAAAAACAGTATTCTCATATTTGATGAAGCTCACAACCTGGAAAGCATATGTGCAGATGCGGCTTCTTTTGACTTGCCATCTTGGCTTCTTACAGCATGCATTTCTGAAGCGAAAAATTGTATTGACCTTTCTGTACAAAGAAGGGATGAATCAACCGATAAATCAAAAAATCCAGATAACTTTGCAATTCTTAGAGCGCTTCTTTTGAAGCTTGAGAAGCGAATTTCTGAAGTGCCTATTCAATCCAAGGAATTGGGATACACTAAGCCTGGAGCATATATCTATGAACTCCTTGCTGATCTAAACATCACACATGATACTGTTCCTATGATCATTAATATAATTGAGGAAGCTACTCTACTTATTGAGGAAGATAACCAGCAGAATGCTAAACGCAATGTCTGCCGGCTGGAAAGCATTAGTGACATCCTGAATACAATTTTCAGAGATATAAGCAATGCAAAATCCTATCGTGTTCATGTACAGGAAGTTCAAGGAAGTGCAACAGATGGTTTAATGGGTAAGTCATCTAGGACACTCAGCTGGTGGTGCTTTCATCCTGGAATTGCAATGGAGGAATTCTCCAAGTTGGATGTAGGCTCTATTATATTGACATCTGGCACACTATCTCCCTTAGAATCATTTGCACAGGAGCTGAAAATAGAGTTTCCTGTGCGGTTGGAAAATCCTCATGTTATTACCCCTAATCAGATATGGGCTGGAGTCGTTCCAGCTGGCCCTTCGGGTTGCTCTTTCAACTCCTCTTATAGAACTCGTGACTCGATTGAGTATAAGCAGGAACTTGGAAATGCTATTGTCAATTTTGCTCGAATTGTACCTGATGGATTGCTTGTATTCTTTCCTTCTTACTACCTTTTGGATCAATGCATTGGGTGCTGGAAGAATATGAGCCATGCCAATTCAACTACAATATGGGAAAGAATTTCCAAACACAAGAAACCTGTTGTAGAACCTAGACAATCTTCATTATTTCTTTCATCAATTGAGGACTATATGGCTAAGCTGAAGGACACCTCAGCTTCTGGTGCAATATTTTTTGCCGTTTGCCGTGGTAAGGTAAGTGAAGGATTAGATTTTGCCGATCACGCTGGAAGAGCTGTAATTGTTACTGGTATGCCATTTGCCACGTGGACTGATCCTAAGGTTCGTCTGAAACGTGAGTACTTAGATGAACAGGCACATTCTCAACGGCAAGTATGTAAGGTGCAGGTTCTGACTGGCGAAGACTGGTACAATCAACAAGCATCACGCGCAGTGAATCAGGCTGTTGGACGTGTAATCCGTCATCGTCATGATTATGGAGCAATTATTTTTCTTGATGAAAGGTTTGCACAGCCAAATCGTCAATCTCAAATATCTCTCTGGATACGACCTCATATCAAGTGTTACTCCAAATTTGGGGATGTGGTTTTTACATTAACCCGTTTCTTCCGGGATGGAGAAGCTCGGGGTCCTACAAAGCTGAATTTATTAACAACTGAAAAACAGGGAAAGATATATGAGTCGGAAATTCAGCAACCTTCAGACAAATTTTATTTTGATAAATTTCTCCCTGGGAATATACAAGTGGGTCAAGGCTGTCCTGTCAAGTTATCATCTTCCATTACTGAGGCTAGAAGGGAAAAAGTGTCGAGCCTGGTGGGTGAAATTGTCCCTGCTAATCGCTCTTCTGTTACTTGCTGCAAGGATCAGTCTGTGTCATGGAAGTGCTCAAATAACCTATTAGGAAATGAGAAAAAGCTTCTTTCCCTTAAGAGGGAGAGTATGCAGTATCAAGATAGAAAAGTAATTAATCTTACTGATGAATCAATATTAAATGAAAAGCGGAGTGAAGAACTGATGACGCCTTGTTCTGCGAAGAAGAGTAAAGTACGCGCAGAACACGATGCAGCACAACATATTAAAAATTCTATTGAGCATTCATCTCTTACAAAAGATTTTGAATCGCATGGCCTTCTTTCCACTATAAGTTCAATGAGAAGCGAAAGCTCACATAGATCTAAGTCATCTGATAGAGAAACTACTCAAAATGCTCAAGCTGGTTCAAAGCTGCTGCATAAAGACAATAGTGTAACATCGAGTCCTGTGCCTTCTGGTGATGAGGAAACGAGAGGTTCTCAATTTCTTGTTCAGGTTAAAGAGAAACTTAGTACTTTGGAGTATAAAGAATTTGTAAATTTGATGAAGGCACTGAAGTCCAAAGCAATGAACATAAGTGAAGTTTTGCAATCAATTGCGAGATTGTTTTCTGGACCAGAGAGGCTTCCCCTTCTTAAAAGATTTAAGTATTTTATCCCAGCAAAGTATCATTCCATGTATGAGCACTATTTTCAAACCAATGGATCTTCTCAGAGTGAGATGCACAACAAGAAGGCCGGTTTTTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006304 GO:0006307 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008198 GO:0009451 GO:0009987 GO:0016070 GO:0016491 GO:0016705 GO:0016706 GO:0032451 GO:0033554 GO:0034641 GO:0035510 GO:0035511 GO:0035513 GO:0035515 GO:0035552 GO:0035553 GO:0042245 GO:0043167 GO:0043169 GO:0043170 GO:0043412 GO:0043734 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0046483 GO:0046872 GO:0046914 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0070579 GO:0070988 GO:0070989 GO:0071704 GO:0080111 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy_2

Protein Analysis

1039

Amino Acids

117.48

Weight (kDa)

8.88

Isoelectric Point (pI)

48.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	103 - 263	4.4e-54	DEAD_2
ARCH_RTEL1	PF23109	269 - 424	9.8e-41	RTEL1 helicase, ARCH domain
Helicase_C_2	PF13307	509 - 702	3.7e-49	Helicase C-terminal domain
HHD_RTEL1	PF23116	900 - 1021	3.8e-12	RTEL1, HHD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007047)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14140 AT3G14160
fragaria_vesca	FvH4_6g35240 FvH4_6g35240
malus_domestica	MD09G1177300.v1.1
prunus_persica	Prupe.3G036700_v2.0.a1 Prupe.3G036700_v2.0.a1 Prupe.3G036700_v2.0.a1 Prupe.3G036700_v2.0.a1
pyrus_communis	pycom09g09550
rosa_chinensis	RchiOBHm_Chr2g0146601
rosa_laevigata	RLG00000020209
rosa_multiflora	Rmu_sc0001986.1_g000030
rosa_roxburghii	Rroxscaffold_2G00100020
rosa_rugosa	Rorug02G0395100 Rorug02G0395200 Rorug02G0395300 Rorug02G0395400 Rorug02G0395500 Rorug02G0395600
rosa_samantha	Rh2AG451800 Rh2BG464600 Rh2CG438700 Rh2DG473600
rosa_wichuraiana	Rw2G036910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1930
AccII	CGCG	3 cut(s)	681, 1983, 2607
AciI	CCGC	5 cut(s)	316, 681, 770, 1387, 2557
AclWI	GGATC	6 cut(s)	325, 633, 1599, 1862, 2427, 3082
AcuI	CTGAAG	5 cut(s)	837, 960, 1757, 2238, 2945
AcvI	CACGTG	1 cut(s)	1860
AcyI	GRCGYC	1 cut(s)	2576
AfeI	AGCGCT	1 cut(s)	909
AfiI	CCNNNNNNNGG	5 cut(s)	46, 1786, 2130, 2487, 2488
AflII	CTTAAG	1 cut(s)	2483
AflIII	ACRYGT	1 cut(s)	2005
AjiI	CACGTC	1 cut(s)	2006
AjnI	CCWGG	5 cut(s)	747, 979, 1270, 2284, 2367
Alw21I	GWGCWC	2 cut(s)	2444, 3057
Alw26I	GTCTC	1 cut(s)	96
AlwI	GGATC	6 cut(s)	325, 633, 1599, 1862, 2427, 3082
AlwNI	CAGNNNCTG	3 cut(s)	1366, 1754, 1945
Ama87I	CYCGRG	3 cut(s)	396, 621, 2178
Aor51HI	AGCGCT	1 cut(s)	909
AoxI	GGCC	6 cut(s)	48, 371, 599, 1447, 2683, 3105
ApeKI	GCWGC	4 cut(s)	2412, 2621, 2782, 2785
AseI	ATTAAT	3 cut(s)	705, 1047, 2523
AspLEI	GCGC	3 cut(s)	910, 1985, 2609
AspS9I	GGNCC	5 cut(s)	206, 599, 1448, 2183, 2984
AsuC2I	CCSGG	3 cut(s)	622, 623, 2165
AsuHPI	GGTGA	3 cut(s)	248, 2387, 2840
AvaI	CYCGRG	3 cut(s)	396, 621, 2178
AvaII	GGWCC	3 cut(s)	206, 2183, 2984
AxyI	CCTNAGG	1 cut(s)	1872
BaeGI	GKGCMC	1 cut(s)	608
BarI	GAAGNNNNNNTAC	2 cut(s)	1053, 1085
BauI	CACGAG	1 cut(s)	1485
BbrPI	CACGTG	1 cut(s)	1860
BbsI	GAAGAC	2 cut(s)	146, 1962
Bbv12I	GWGCWC	2 cut(s)	2444, 3057
BbvCI	CCTCAGC	1 cut(s)	1747
BbvI	GCAGC	4 cut(s)	2399, 2633, 2769, 2772
BccI	CCATC	4 cut(s)	796, 1214, 1547, 2162
BceAI	ACGGC	4 cut(s)	586, 1757, 1764, 1937
BcgI	CGANNNNNNTGC	4 cut(s)	13, 47, 2800, 2834
BciT130I	CCWGG	5 cut(s)	749, 981, 1272, 2286, 2369
BciVI	GTATCC	2 cut(s)	962, 2091
BclI	TGATCA	1 cut(s)	1041
BcnI	CCSGG	3 cut(s)	622, 623, 2165
BcoDI	GTCTC	1 cut(s)	96
BfaI	CTAG	3 cut(s)	1242, 1686, 2346
BfmI	CTRYAG	1 cut(s)	472
BfoI	RGCGCY	1 cut(s)	911
BfrI	CTTAAG	1 cut(s)	2483
BfuAI	ACCTGC	1 cut(s)	1930
BfuI	GTATCC	2 cut(s)	962, 2091
BglI	GCCNNNNNGGC	2 cut(s)	170, 793
BglII	AGATCT	2 cut(s)	104, 2729
BisI	GCNGC	5 cut(s)	771, 2413, 2622, 2783, 2786
BlpI	GCTNAGC	1 cut(s)	1731
BlsI	GCNGC	5 cut(s)	772, 2414, 2623, 2784, 2787
BmcAI	AGTACT	2 cut(s)	1895, 2884
Bme1390I	CCNGG	8 cut(s)	622, 623, 749, 981, 1272, 2165, 2286, 2369
Bme18I	GGWCC	3 cut(s)	206, 2183, 2984
BmeT110I	CYCGRG	3 cut(s)	396, 621, 2178
BmgBI	CACGTC	1 cut(s)	2006
BmgT120I	GGNCC	5 cut(s)	206, 599, 1448, 2183, 2984
BmiI	GGNNCC	1 cut(s)	2184
BmrFI	CCNGG	8 cut(s)	622, 623, 749, 981, 1272, 2165, 2286, 2369
BmrI	ACTGGG	4 cut(s)	147, 256, 262, 268
BmsI	GCATC	5 cut(s)	33, 757, 1985, 2608, 3081
BmuI	ACTGGG	4 cut(s)	147, 256, 262, 268
BpiI	GAAGAC	2 cut(s)	146, 1962
BplI	GAGNNNNNCTC	4 cut(s)	1277, 1309, 2833, 2865
BpmI	CTGGAG	2 cut(s)	1002, 1452
Bpu10I	CCTNAGC	1 cut(s)	1747
Bpu1102I	GCTNAGC	1 cut(s)	1731
BpuEI	CTTGAG	2 cut(s)	944, 2751
BpuMI	CCSGG	3 cut(s)	622, 623, 2165
Bsa29I	ATCGAT	1 cut(s)	27
BsaAI	YACGTR	1 cut(s)	1860
BsaHI	GRCGYC	1 cut(s)	2576
BsaJI	CCNNGG	5 cut(s)	621, 957, 1780, 2284, 2285
BsaXI	ACNNNNNCTCC	2 cut(s)	1324, 1354
Bsc4I	CCNNNNNNNGG	5 cut(s)	46, 1786, 2130, 2487, 2488
Bse118I	RCCGGY	2 cut(s)	1116, 3107
Bse21I	CCTNAGG	1 cut(s)	1872
Bse3DI	GCAATG	4 cut(s)	1114, 1174, 1287, 2943
BseBI	CCWGG	5 cut(s)	749, 981, 1272, 2286, 2369
BseCI	ATCGAT	1 cut(s)	27
BseDI	CCNNGG	5 cut(s)	621, 957, 1780, 2284, 2285
BseGI	GGATG	7 cut(s)	292, 862, 1137, 1267, 1309, 2140, 2173
BseLI	CCNNNNNNNGG	5 cut(s)	46, 1786, 2130, 2487, 2488
BseMI	GCAATG	4 cut(s)	1114, 1174, 1287, 2943
BseMII	CTCAG	4 cut(s)	1264, 1761, 2331, 3095
BseRI	GAGGAG	1 cut(s)	1462
BseSI	GKGCMC	1 cut(s)	608
BseXI	GCAGC	4 cut(s)	2399, 2633, 2769, 2772
BseYI	CCCAGC	1 cut(s)	3028
BsgI	GTGCAG	2 cut(s)	783, 1958
Bsh1236I	CGCG	3 cut(s)	681, 1983, 2607
BshFI	GGCC	6 cut(s)	50, 373, 601, 1449, 2685, 3107
BshVI	ATCGAT	1 cut(s)	27
BsiHKAI	GWGCWC	2 cut(s)	2444, 3057
BsiHKCI	CYCGRG	3 cut(s)	396, 621, 2178
BsiSI	CCGG	5 cut(s)	334, 622, 1117, 2164, 3108
BslFI	GGGAC	6 cut(s)	244, 250, 256, 262, 298, 2369
BslI	CCNNNNNNNGG	5 cut(s)	46, 1786, 2130, 2487, 2488
BsmAI	GTCTC	1 cut(s)	96
BsmFI	GGGAC	6 cut(s)	244, 250, 256, 262, 298, 2369
BsmI	GAATGC	4 cut(s)	411, 460, 1102, 2650
BsnI	GGCC	6 cut(s)	50, 373, 601, 1449, 2685, 3107
BsoBI	CYCGRG	3 cut(s)	396, 621, 2178
Bsp1286I	GDGCHC	3 cut(s)	608, 2444, 3057
Bsp1407I	TGTACA	2 cut(s)	844, 1192
Bsp1720I	GCTNAGC	1 cut(s)	1731
BspACI	CCGC	5 cut(s)	316, 681, 770, 1387, 2557
BspANI	GGCC	6 cut(s)	50, 373, 601, 1449, 2685, 3107
BspCNI	CTCAG	4 cut(s)	1263, 1760, 2332, 3094
BspDI	ATCGAT	1 cut(s)	27
BspFNI	CGCG	3 cut(s)	681, 1983, 2607
BspHI	TCATGA	2 cut(s)	415, 2023
BspLI	GGNNCC	1 cut(s)	2184
BspMI	ACCTGC	1 cut(s)	1930
BspPI	GGATC	6 cut(s)	325, 633, 1599, 1862, 2427, 3082
BspQI	GCTCTTC	3 cut(s)	176, 1819, 2404
BspTI	CTTAAG	1 cut(s)	2483
BsrDI	GCAATG	4 cut(s)	1114, 1174, 1287, 2943
BsrFI	RCCGGY	2 cut(s)	1116, 3107
BsrGI	TGTACA	2 cut(s)	844, 1192
BssAI	RCCGGY	2 cut(s)	1116, 3107
BssECI	CCNNGG	5 cut(s)	621, 957, 1780, 2284, 2285
BssNI	GRCGYC	1 cut(s)	2576
BssSI	CACGAG	1 cut(s)	1485
BssT1I	CCWWGG	1 cut(s)	957
Bst2BI	CACGAG	1 cut(s)	1485
Bst2UI	CCWGG	5 cut(s)	749, 981, 1272, 2286, 2369
Bst4CI	ACNGT	4 cut(s)	14, 117, 719, 1033
Bst6I	CTCTTC	4 cut(s)	176, 1819, 2404, 2588
BstACI	GRCGYC	1 cut(s)	2576
BstAFI	CTTAAG	1 cut(s)	2483
BstAPI	GCANNNNNTGC	2 cut(s)	1113, 1605
BstAUI	TGTACA	2 cut(s)	844, 1192
BstBAI	YACGTR	1 cut(s)	1860
BstC8I	GCNNGC	4 cut(s)	62, 808, 1118, 1447
BstDSI	CCRYGG	1 cut(s)	1780
BstF5I	GGATG	7 cut(s)	292, 862, 1137, 1267, 1309, 2140, 2173
BstFNI	CGCG	3 cut(s)	681, 1983, 2607
BstH2I	RGCGCY	1 cut(s)	911
BstHHI	GCGC	3 cut(s)	910, 1985, 2609
BstMAI	GTCTC	1 cut(s)	96
BstNI	CCWGG	5 cut(s)	749, 981, 1272, 2286, 2369
BstNSI	RCATGY	1 cut(s)	810
BstSCI	CCNGG	8 cut(s)	620, 621, 747, 979, 1270, 2163, 2284, 2367
BstSFI	CTRYAG	1 cut(s)	472
BstSLI	GKGCMC	1 cut(s)	608
BstUI	CGCG	3 cut(s)	681, 1983, 2607
BstV1I	GCAGC	4 cut(s)	2399, 2633, 2769, 2772
BstV2I	GAAGAC	2 cut(s)	146, 1962
BstX2I	RGATCY	5 cut(s)	104, 330, 625, 2729, 3074
BstXI	CCANNNNNNTGG	1 cut(s)	964
BstYI	RGATCY	5 cut(s)	104, 330, 625, 2729, 3074
Bsu15I	ATCGAT	1 cut(s)	27
Bsu36I	CCTNAGG	1 cut(s)	1872
BsuI	GTATCC	2 cut(s)	962, 2091
BsuRI	GGCC	6 cut(s)	50, 373, 601, 1449, 2685, 3107
BsuTUI	ATCGAT	1 cut(s)	27
BtgI	CCRYGG	1 cut(s)	1780
BtrI	CACGTC	1 cut(s)	2006
BtsCI	GGATG	7 cut(s)	292, 862, 1137, 1267, 1309, 2140, 2173
BtsI	GCAGTG	1 cut(s)	1992
BtsIMutI	CAGTG	3 cut(s)	113, 1992, 2921
BveI	ACCTGC	1 cut(s)	1930
Cac8I	GCNNGC	4 cut(s)	62, 808, 1118, 1447
CaiI	CAGNNNCTG	3 cut(s)	1366, 1754, 1945
CciI	TCATGA	2 cut(s)	415, 2023
CfoI	GCGC	3 cut(s)	910, 1985, 2609
Cfr10I	RCCGGY	2 cut(s)	1116, 3107
Cfr13I	GGNCC	5 cut(s)	206, 599, 1448, 2183, 2984
Cfr9I	CCCGGG	1 cut(s)	621
ClaI	ATCGAT	1 cut(s)	27
CseI	GACGC	2 cut(s)	300, 2584
Eam1104I	CTCTTC	4 cut(s)	176, 1819, 2404, 2588
EarI	CTCTTC	4 cut(s)	176, 1819, 2404, 2588
Eco130I	CCWWGG	1 cut(s)	957
Eco147I	AGGCCT	1 cut(s)	50
Eco47I	GGWCC	3 cut(s)	206, 2183, 2984
Eco47III	AGCGCT	1 cut(s)	909
Eco57I	CTGAAG	5 cut(s)	837, 960, 1757, 2238, 2945
Eco72I	CACGTG	1 cut(s)	1860
Eco81I	CCTNAGG	1 cut(s)	1872
Eco88I	CYCGRG	3 cut(s)	396, 621, 2178
EcoO109I	RGGNCCY	1 cut(s)	2183
EcoRI	GAATTC	1 cut(s)	1289
EcoRII	CCWGG	5 cut(s)	747, 979, 1270, 2284, 2367
EcoT14I	CCWWGG	1 cut(s)	957
EcoT22I	ATGCAT	3 cut(s)	413, 812, 1601
ErhI	CCWWGG	1 cut(s)	957
FalI	AAGNNNNNCTT	4 cut(s)	349, 381, 2862, 2894
FaqI	GGGAC	6 cut(s)	244, 250, 256, 262, 298, 2369
FauI	CCCGC	2 cut(s)	323, 674
FauNDI	CATATG	1 cut(s)	758
FbaI	TGATCA	1 cut(s)	1041
Fnu4HI	GCNGC	5 cut(s)	771, 2413, 2622, 2783, 2786
FokI	GGATG	7 cut(s)	279, 869, 1124, 1254, 1316, 2147, 2180
Fsp4HI	GCNGC	5 cut(s)	771, 2413, 2622, 2783, 2786
FspBI	CTAG	3 cut(s)	1242, 1686, 2346
GlaI	GCGC	3 cut(s)	909, 1984, 2608
GluI	GCNGC	5 cut(s)	771, 2413, 2622, 2783, 2786
GsaI	CCCAGC	1 cut(s)	3032
GsuI	CTGGAG	2 cut(s)	1002, 1452
HaeII	RGCGCY	1 cut(s)	911
HaeIII	GGCC	6 cut(s)	50, 373, 601, 1449, 2685, 3107
HapII	CCGG	5 cut(s)	334, 622, 1117, 2164, 3108
HgaI	GACGC	2 cut(s)	300, 2584
HhaI	GCGC	3 cut(s)	910, 1985, 2609
Hin1I	GRCGYC	1 cut(s)	2576
Hin6I	GCGC	3 cut(s)	908, 1983, 2607
HinP1I	GCGC	3 cut(s)	908, 1983, 2607
HindIII	AAGCTT	2 cut(s)	920, 2471
HpaII	CCGG	5 cut(s)	334, 622, 1117, 2164, 3108
HphI	GGTGA	3 cut(s)	248, 2387, 2840
Hpy166II	GTNNAC	3 cut(s)	70, 431, 1863
Hpy8I	GTNNAC	3 cut(s)	70, 431, 1863
HpyCH4III	ACNGT	4 cut(s)	14, 117, 719, 1033
HpyCH4IV	ACGT	3 cut(s)	1859, 1888, 2005
HpySE526I	ACGT	3 cut(s)	1859, 1888, 2005
Hsp92I	GRCGYC	1 cut(s)	2576
HspAI	GCGC	3 cut(s)	908, 1983, 2607
KroI	GCCGGC	1 cut(s)	1116
KroNI	GCCGGC	1 cut(s)	1118
Ksp22I	TGATCA	1 cut(s)	1041
LguI	GCTCTTC	3 cut(s)	176, 1819, 2404
LmnI	GCTCC	3 cut(s)	983, 1363, 2033
Lsp1109I	GCAGC	4 cut(s)	2399, 2633, 2769, 2772
LweI	GCATC	5 cut(s)	33, 757, 1985, 2608, 3081
MaeI	CTAG	3 cut(s)	1242, 1686, 2346
MaeII	ACGT	3 cut(s)	1859, 1888, 2005
MaeIII	GTNAC	7 cut(s)	461, 1133, 1487, 1837, 2117, 2404, 2803
MfeI	CAATTG	2 cut(s)	1713, 2964
MflI	RGATCY	5 cut(s)	104, 330, 625, 2729, 3074
MhlI	GDGCHC	3 cut(s)	608, 2444, 3057
MlyI	GAGTC	6 cut(s)	133, 232, 1443, 1484, 2243, 2821
MmeI	TCCRAC	7 cut(s)	99, 121, 152, 1281, 1371, 1981, 2217
Mph1103I	ATGCAT	3 cut(s)	413, 812, 1601
MroNI	GCCGGC	1 cut(s)	1116
MslI	CAYNNNNRTG	3 cut(s)	2022, 2028, 2437
MspA1I	CMGCKG	2 cut(s)	1254, 1445
MspCI	CTTAAG	1 cut(s)	2483
MspI	CCGG	5 cut(s)	334, 622, 1117, 2164, 3108
MspR9I	CCNGG	8 cut(s)	622, 623, 749, 981, 1272, 2165, 2286, 2369
MunI	CAATTG	2 cut(s)	1713, 2964
Mva1269I	GAATGC	4 cut(s)	411, 460, 1102, 2650
MvaI	CCWGG	5 cut(s)	749, 981, 1272, 2286, 2369
MvnI	CGCG	3 cut(s)	681, 1983, 2607
NaeI	GCCGGC	1 cut(s)	1118
NciI	CCSGG	3 cut(s)	622, 623, 2165
NdeI	CATATG	1 cut(s)	758
NgoMIV	GCCGGC	1 cut(s)	1116
NlaIV	GGNNCC	1 cut(s)	2184
NmuCI	GTSAC	3 cut(s)	461, 1133, 1487
NsiI	ATGCAT	3 cut(s)	413, 812, 1601
NspI	RCATGY	1 cut(s)	810
PaeI	GCATGC	1 cut(s)	810
PaeR7I	CTCGAG	1 cut(s)	396
PagI	TCATGA	2 cut(s)	415, 2023
PasI	CCCWGGG	1 cut(s)	2285
PceI	AGGCCT	1 cut(s)	50
PciSI	GCTCTTC	3 cut(s)	176, 1819, 2404
PcsI	WCGNNNNNNNCGW	3 cut(s)	51, 1885, 2011
PctI	GAATGC	4 cut(s)	411, 460, 1102, 2650
PdiI	GCCGGC	1 cut(s)	1118
PfeI	GAWTC	5 cut(s)	860, 1349, 1990, 2537, 2675
PfoI	TCCNGGA	2 cut(s)	1270, 2163
PkrI	GCNGC	5 cut(s)	772, 2414, 2623, 2784, 2787
PleI	GAGTC	6 cut(s)	132, 231, 1442, 1484, 2242, 2820
PmaCI	CACGTG	1 cut(s)	1860
PmlI	CACGTG	1 cut(s)	1860
PpsI	GAGTC	6 cut(s)	132, 231, 1442, 1484, 2242, 2820
Ppu21I	YACGTR	1 cut(s)	1860
PpuMI	RGGWCCY	1 cut(s)	2183
PshBI	ATTAAT	3 cut(s)	705, 1047, 2523
Psp5II	RGGWCCY	1 cut(s)	2183
Psp6I	CCWGG	5 cut(s)	747, 979, 1270, 2284, 2367
PspCI	CACGTG	1 cut(s)	1860
PspFI	CCCAGC	1 cut(s)	3028
PspGI	CCWGG	5 cut(s)	747, 979, 1270, 2284, 2367
PspN4I	GGNNCC	1 cut(s)	2184
PspPI	GGNCC	5 cut(s)	206, 599, 1448, 2183, 2984
PspPPI	RGGWCCY	1 cut(s)	2183
PsrI	GAACNNNNNNTAC	2 cut(s)	1185, 1217
PstNI	CAGNNNCTG	3 cut(s)	1366, 1754, 1945
PsuI	RGATCY	5 cut(s)	104, 330, 625, 2729, 3074
PvuII	CAGCTG	2 cut(s)	1254, 1445
RseI	CAYNNNNRTG	3 cut(s)	2022, 2028, 2437
SapI	GCTCTTC	3 cut(s)	176, 1819, 2404
SatI	GCNGC	5 cut(s)	771, 2413, 2622, 2783, 2786
Sau96I	GGNCC	5 cut(s)	206, 599, 1448, 2183, 2984
ScaI	AGTACT	2 cut(s)	1895, 2884
SchI	GAGTC	6 cut(s)	133, 232, 1443, 1484, 2243, 2821
ScrFI	CCNGG	8 cut(s)	622, 623, 749, 981, 1272, 2165, 2286, 2369
SduI	GDGCHC	3 cut(s)	608, 2444, 3057
SfaNI	GCATC	5 cut(s)	33, 757, 1985, 2608, 3081
SfcI	CTRYAG	1 cut(s)	472
Sfr274I	CTCGAG	1 cut(s)	396
SinI	GGWCC	3 cut(s)	206, 2183, 2984
SlaI	CTCGAG	1 cut(s)	396
SmaI	CCCGGG	1 cut(s)	623
SmiMI	CAYNNNNRTG	3 cut(s)	2022, 2028, 2437
SmlI	CTYRAG	4 cut(s)	396, 923, 2483, 2766
SmoI	CTYRAG	4 cut(s)	396, 923, 2483, 2766
SphI	GCATGC	1 cut(s)	810
SseBI	AGGCCT	1 cut(s)	50
SsiI	CCGC	5 cut(s)	316, 681, 770, 1387, 2557
SspI	AATATT	2 cut(s)	1764, 2544
SspMI	CTAG	3 cut(s)	1242, 1686, 2346
StuI	AGGCCT	1 cut(s)	50
StyD4I	CCNGG	8 cut(s)	620, 621, 747, 979, 1270, 2163, 2284, 2367
StyI	CCWWGG	1 cut(s)	957
TaaI	ACNGT	4 cut(s)	14, 117, 719, 1033
TaiI	ACGT	3 cut(s)	1862, 1891, 2008
TaqI	TCGA	8 cut(s)	27, 33, 45, 397, 1493, 1540, 2363, 2810
TatI	WGTACW	4 cut(s)	844, 1192, 1893, 2882
TauI	GCSGC	1 cut(s)	773
TfiI	GAWTC	5 cut(s)	860, 1349, 1990, 2537, 2675
TscAI	CASTG	3 cut(s)	120, 1992, 2928
TseFI	GTSAC	3 cut(s)	461, 1133, 1487
TseI	GCWGC	4 cut(s)	2412, 2621, 2782, 2785
Tsp45I	GTSAC	3 cut(s)	461, 1133, 1487
TspGWI	ACGGA	1 cut(s)	2003
TspMI	CCCGGG	1 cut(s)	621
TspRI	CASTG	3 cut(s)	120, 1992, 2928
Vha464I	CTTAAG	1 cut(s)	2483
VpaK11BI	GGWCC	3 cut(s)	206, 2183, 2984
VspI	ATTAAT	3 cut(s)	705, 1047, 2523
XceI	RCATGY	1 cut(s)	810
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1857
XhoI	CTCGAG	1 cut(s)	396
XmaI	CCCGGG	1 cut(s)	621
XspI	CTAG	3 cut(s)	1242, 1686, 2346
ZrmI	AGTACT	2 cut(s)	1895, 2884
Zsp2I	ATGCAT	3 cut(s)	413, 812, 1601

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.