pycom09g12830

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005377
Physical Location & Seq
Forward (+)
11374758 .. 11379109
4352 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2025 bp
ATGAAAATGGAAGAGGAAACAGAAAAGGAGGCGGAGTCCCCAAAATTCCCAGCATTTCCGTACAAACCCTACGACATACAGATCGATTTCATGAAGGCCCTCTATCACTCTCTCAACAAGGGAGGCCTTTCCATGCTCGAAAGCCCCACCGGGACTGGGAAAACTCTAAGTATCATTTGTAGTGCTTTGCAATGGGTGGTTGATAGGAGGGAACAACAGAAGTCTGAAAATAGTGTTGAATCTGATCTTAATAGTGCTAAAGATGGTCGAGCTGACTCGGATGATGAGCCTGACTGGATAAGAAATGTTGTTATTAACAAAGACAAGCAGAGTGAGGAGAAGAAGACTAATAAGAAGGAGAAATTTGGGGTTGGGTTTCGAAGGGCCGATAAGAGGGGGAGCCGCGGAAATTGTAGAGATTTGTTCTCGCAGAGTATTGAGGAGGAGCCTTCCGCAAAGAAAGAGGAAAGGAATTTGCTAATGAGCAATGATGCTGTGGAGTTAAGTGATGAGGAGTTTTTGGTTGGAGATTATGAAAGCGAAGAGGAAGGGGATCCGGGTGGTGGGAAGTCAAAGAGGAAGCTCTCTGGGGTTTCTCTTAGTTCGTCAAGTGAGGAAGATGAAGAAAATGGGTCTGGTGACGATGAATTTGAGGATGAGAAGCTGCAGGTTTATTTTTGTAGCCGGACACATTCACAGCTTTCACAGTTCATAAAGGAGTTAAGGAAGACAATCTTTGCTAATAAGATGAAGGTGGTGTCTTTGGGCTCAAGGAAGAATTGCTGCATCAATGAAGAGGTATTGAAACTTGGAAACTCAACTCGCATCAATGAACGCTGTTTGGAACTTCAAAAGAACAAGAATAAGGATGTTTCCAAAATCAAGACCTTAGGTGCCAGAGTCAGGATTCGCAGGAACAAGGCTTCTTGTGGATGCCCGATGCTTAGAAAACATAAAGTACAAAAAGAATTCAGGAGCATGATGTCTCAACATGGTGCCTTGGATATTGAAGATCTTGTTGATCTTGGAAAAAGCATGAGGACTTGTCCATATTATGGCTCCCGAAGCATGGTTCCAGTTGCTGATCTAGTGGTTCTACCGTATCAGTCTCTTCTTTCTAAAGCATCTCGCGAGTCTCTTGGTCTCAAGTTAAAGAATAATATTGTTATTATAGATGAGGCTCACAATCTAGCTGACTCCTTGATCAGCATGTATGACTCAAGAATCACTTTGTCGCAGCTGGAGGATGTGCACTGGCACATTGAGAAGTACTTTGCAAGATTTTGCAATGTATTGGGACCTGGTAATCGTAGATATATTCAAACTCTCATGGTGCTCACTCGGGCTTTCCTCCAAGTGTTTCTAAGAGAGGATACGGGCTGCGGAATTTCTTGTCATGATACTGAAAAGGCTTCTGGAGCAAATGGAACGCCTACTTCCTCTATGGCCATTAATGACTTTTTATTTTCCCTCAATATAGACAACATCAACTTGGTCAAATTGCTTCAGTATATAAAGGAAAGCAACATCATGCACAAGGTCAGTGGTTATGGCGATAAAGTAGCTATTCAAAAGAGTTTGGCATTTAATGGCGGTAAAGAGAGAAGAACTTTGTCCAGTTTCCAAGCATTAGCAAATTTTTTGCTATCACTGACAAATAAAGATGGAGATGGGAGGATTATAATCTCAAAGACTAGTCCAACATGCCCTGGAGAGGAAGGAGGATATATAAAATATGTAATGCTTACTGGGGATAATATTTTTTCAGAGATTGTGGATCAAGCACATGCTGTTGTACTGGCTGGGGGAACTCTCCAACCTATAGAAGAAACAAGGGAGAGACTCTTCCCTTGGTTACCACTGAACCAGTTGCAATTCTTTTCATGCAGCCATATTGTTCCCCCAGAGAGCATTCTGCCAGTTGCAGTTTCCCGTGGTCCTTCTGGTCACACTTTTGACTTTAGCTACAGCTCCAGGAGGTCATCAATCACGTTCTCTCTTTTTAGTGCATCCGTAATTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD_2 Helicase_C_2

Protein Analysis

675

Amino Acids

75.67

Weight (kDa)

6.68

Isoelectric Point (pI)

60.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD_2 PF06733 226 - 414 5e-53 DEAD_2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1682
Acc36I ACCTGC 1 cut(s) 658
AccB1I GGYRCC 2 cut(s) 893, 995
AccB7I CCANNNNNTGG 1 cut(s) 1055
AccII CGCG 2 cut(s) 405, 1131
AciI CCGC 6 cut(s) 32, 403, 405, 453, 1383, 1593
AclWI GGATC 3 cut(s) 548, 561, 1785
AcoI YGGCCR 1 cut(s) 1446
AcsI RAATTY 7 cut(s) 44, 362, 472, 647, 968, 1386, 1636
AcuI CTGAAG 1 cut(s) 1490
AfaI GTAC 4 cut(s) 62, 960, 1271, 1797
AfiI CCNNNNNNNGG 7 cut(s) 156, 393, 563, 903, 1055, 1069, 1714
AgsI TTSAA 6 cut(s) 239, 805, 851, 1010, 1322, 1571
AhlI ACTAGT 1 cut(s) 1694
AjnI CCWGG 3 cut(s) 1300, 1708, 1973
AluBI AGCT 9 cut(s) 272, 583, 664, 700, 1193, 1240, 1565, 1965, 1971
AluI AGCT 9 cut(s) 272, 583, 664, 700, 1193, 1240, 1565, 1965, 1971
Alw21I GWGCWC 2 cut(s) 1254, 1338
Alw26I GTCTC 5 cut(s) 990, 1113, 1140, 1148, 1834
Alw44I GTGCAC 1 cut(s) 1250
AlwI GGATC 3 cut(s) 548, 561, 1785
AlwNI CAGNNNCTG 1 cut(s) 1082
Ama87I CYCGRG 1 cut(s) 1341
AoxI GGCC 4 cut(s) 96, 124, 384, 1446
ApaLI GTGCAC 1 cut(s) 1250
ApeKI GCWGC 5 cut(s) 664, 783, 1237, 1380, 1887
ApoI RAATTY 7 cut(s) 44, 362, 472, 647, 968, 1386, 1636
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AseI ATTAAT 1 cut(s) 1452
AspS9I GGNCC 4 cut(s) 97, 384, 1298, 1937
AsuC2I CCSGG 2 cut(s) 151, 558
AsuHPI GGTGA 1 cut(s) 650
AsuII TTCGAA 1 cut(s) 379
AvaI CYCGRG 1 cut(s) 1341
AvaII GGWCC 2 cut(s) 1298, 1937
AxyI CCTNAGG 1 cut(s) 889
BaeGI GKGCMC 1 cut(s) 1254
BalI TGGCCA 1 cut(s) 1448
BamHI GGATCC 1 cut(s) 553
BanI GGYRCC 2 cut(s) 893, 995
BanII GRGCYC 1 cut(s) 770
BbsI GAAGAC 2 cut(s) 350, 734
Bbv12I GWGCWC 2 cut(s) 1254, 1338
BbvI GCAGC 5 cut(s) 651, 770, 1249, 1367, 1899
BccI CCATC 3 cut(s) 257, 1658, 1664
BciT130I CCWGG 3 cut(s) 1302, 1710, 1975
BciVI GTATCC 1 cut(s) 1366
BclI TGATCA 1 cut(s) 1203
BcnI CCSGG 2 cut(s) 151, 558
BcoDI GTCTC 5 cut(s) 990, 1113, 1140, 1148, 1834
BcuI ACTAGT 1 cut(s) 1694
BfaI CTAG 3 cut(s) 1088, 1190, 1695
BfmI CTRYAG 3 cut(s) 665, 1821, 1966
BfuAI ACCTGC 1 cut(s) 658
BfuI GTATCC 1 cut(s) 1366
BglII AGATCT 1 cut(s) 1012
BisI GCNGC 6 cut(s) 403, 665, 784, 1238, 1381, 1888
BlsI GCNGC 6 cut(s) 404, 666, 785, 1239, 1382, 1889
BmcAI AGTACT 1 cut(s) 1271
Bme1390I CCNGG 5 cut(s) 151, 558, 1302, 1710, 1975
Bme18I GGWCC 2 cut(s) 1298, 1937
BmeT110I CYCGRG 1 cut(s) 1341
BmgT120I GGNCC 4 cut(s) 97, 384, 1298, 1937
BmiI GGNNCC 8 cut(s) 401, 447, 555, 895, 997, 1060, 1074, 1299
BmrFI CCNGG 5 cut(s) 151, 558, 1302, 1710, 1975
BmrI ACTGGG 2 cut(s) 165, 1758
BmsI GCATC 7 cut(s) 481, 795, 834, 923, 930, 1133, 2018
BmuI ACTGGG 2 cut(s) 165, 1758
BpiI GAAGAC 2 cut(s) 350, 734
BpmI CTGGAG 4 cut(s) 1262, 1437, 1731, 1957
Bpu14I TTCGAA 1 cut(s) 379
BpuEI CTTGAG 3 cut(s) 754, 1130, 1204
BpuMI CCSGG 2 cut(s) 151, 558
Bsa29I ATCGAT 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 1682
BsaI GGTCTC 1 cut(s) 1148
BsaJI CCNNGG 5 cut(s) 403, 999, 1708, 1850, 1933
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 7 cut(s) 156, 393, 563, 903, 1055, 1069, 1714
Bse1I ACTGG 9 cut(s) 160, 299, 1076, 1259, 1617, 1753, 1803, 1867, 1919
Bse21I CCTNAGG 1 cut(s) 889
Bse3DI GCAATG 3 cut(s) 197, 493, 1294
Bse8I GATNNNNATC 1 cut(s) 1682
BseBI CCWGG 3 cut(s) 1302, 1710, 1975
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 5 cut(s) 403, 999, 1708, 1850, 1933
BseGI GGATG 6 cut(s) 286, 661, 874, 938, 1252, 2009
BseJI GATNNNNATC 1 cut(s) 1682
BseLI CCNNNNNNNGG 7 cut(s) 156, 393, 563, 903, 1055, 1069, 1714
BseMI GCAATG 3 cut(s) 197, 493, 1294
BseNI ACTGG 9 cut(s) 160, 299, 1076, 1259, 1617, 1753, 1803, 1867, 1919
BseRI GAGGAG 4 cut(s) 350, 455, 458, 527
BseSI GKGCMC 1 cut(s) 1254
BseXI GCAGC 5 cut(s) 651, 770, 1249, 1367, 1899
BseYI CCCAGC 2 cut(s) 49, 1802
Bsh1236I CGCG 2 cut(s) 405, 1131
BshFI GGCC 4 cut(s) 98, 126, 386, 1448
BshNI GGYRCC 2 cut(s) 893, 995
BshVI ATCGAT 1 cut(s) 84
BsiHKAI GWGCWC 2 cut(s) 1254, 1338
BsiHKCI CYCGRG 1 cut(s) 1341
BsiSI CCGG 3 cut(s) 150, 557, 685
BslFI GGGAC 3 cut(s) 22, 166, 1311
BslI CCNNNNNNNGG 7 cut(s) 156, 393, 563, 903, 1055, 1069, 1714
BsmAI GTCTC 5 cut(s) 990, 1113, 1140, 1148, 1834
BsmFI GGGAC 3 cut(s) 22, 166, 1311
BsmI GAATGC 1 cut(s) 1911
BsnI GGCC 4 cut(s) 98, 126, 386, 1448
Bso31I GGTCTC 1 cut(s) 1148
BsoBI CYCGRG 1 cut(s) 1341
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 3 cut(s) 770, 1254, 1338
Bsp143I GATC 8 cut(s) 81, 244, 553, 1012, 1021, 1084, 1203, 1777
Bsp68I TCGCGA 1 cut(s) 1131
BspACI CCGC 6 cut(s) 32, 403, 405, 453, 1383, 1593
BspANI GGCC 4 cut(s) 98, 126, 386, 1448
BspDI ATCGAT 1 cut(s) 84
BspFNI CGCG 2 cut(s) 405, 1131
BspHI TCATGA 2 cut(s) 90, 1396
BspLI GGNNCC 8 cut(s) 401, 447, 555, 895, 997, 1060, 1074, 1299
BspMAI CTGCAG 1 cut(s) 669
BspMI ACCTGC 1 cut(s) 658
BspPI GGATC 3 cut(s) 548, 561, 1785
BspT104I TTCGAA 1 cut(s) 379
BspT107I GGYRCC 2 cut(s) 893, 995
BspTNI GGTCTC 1 cut(s) 1148
BsrDI GCAATG 3 cut(s) 197, 493, 1294
BsrI ACTGG 9 cut(s) 160, 299, 1076, 1259, 1617, 1753, 1803, 1867, 1919
BssECI CCNNGG 5 cut(s) 403, 999, 1708, 1850, 1933
BssMI GATC 8 cut(s) 81, 244, 553, 1012, 1021, 1084, 1203, 1777
BssT1I CCWWGG 2 cut(s) 999, 1850
Bst2UI CCWGG 3 cut(s) 1302, 1710, 1975
Bst4CI ACNGT 2 cut(s) 708, 1101
Bst6I CTCTTC 5 cut(s) 6, 537, 789, 1116, 1850
BstBI TTCGAA 1 cut(s) 379
BstDEI CTNAG 5 cut(s) 167, 599, 889, 944, 1364
BstDSI CCRYGG 2 cut(s) 403, 1933
BstEII GGTNACC 1 cut(s) 1854
BstF5I GGATG 6 cut(s) 286, 661, 874, 938, 1252, 2009
BstFNI CGCG 2 cut(s) 405, 1131
BstKTI GATC 8 cut(s) 84, 247, 556, 1015, 1024, 1087, 1206, 1780
BstMAI GTCTC 5 cut(s) 990, 1113, 1140, 1148, 1834
BstMBI GATC 8 cut(s) 81, 244, 553, 1012, 1021, 1084, 1203, 1777
BstMWI GCNNNNNNNGC 2 cut(s) 1065, 1418
BstNI CCWGG 3 cut(s) 1302, 1710, 1975
BstNSI RCATGY 3 cut(s) 1213, 1707, 1790
BstPI GGTNACC 1 cut(s) 1854
BstSCI CCNGG 5 cut(s) 149, 556, 1300, 1708, 1973
BstSFI CTRYAG 3 cut(s) 665, 1821, 1966
BstSLI GKGCMC 1 cut(s) 1254
BstUI CGCG 2 cut(s) 405, 1131
BstV1I GCAGC 5 cut(s) 651, 770, 1249, 1367, 1899
BstV2I GAAGAC 2 cut(s) 350, 734
BstX2I RGATCY 2 cut(s) 553, 1012
BstYI RGATCY 2 cut(s) 553, 1012
Bsu15I ATCGAT 1 cut(s) 84
Bsu36I CCTNAGG 1 cut(s) 889
BsuI GTATCC 1 cut(s) 1366
BsuRI GGCC 4 cut(s) 98, 126, 386, 1448
BsuTUI ATCGAT 1 cut(s) 84
BtgI CCRYGG 2 cut(s) 403, 1933
BtsCI GGATG 6 cut(s) 286, 661, 874, 938, 1252, 2009
BtsIMutI CAGTG 4 cut(s) 1252, 1549, 1649, 1859
BtuMI TCGCGA 1 cut(s) 1131
BveI ACCTGC 1 cut(s) 658
CaiI CAGNNNCTG 1 cut(s) 1082
CciI TCATGA 2 cut(s) 90, 1396
Cfr13I GGNCC 4 cut(s) 97, 384, 1298, 1937
Cfr42I CCGCGG 1 cut(s) 406
ClaI ATCGAT 1 cut(s) 84
CsiI ACCWGGT 1 cut(s) 1300
Csp6I GTAC 4 cut(s) 61, 959, 1270, 1796
CspCI CAANNNNNGTGG 2 cut(s) 1525, 1560
CviQI GTAC 4 cut(s) 61, 959, 1270, 1796
DdeI CTNAG 5 cut(s) 167, 599, 889, 944, 1364
DpnI GATC 8 cut(s) 83, 246, 555, 1014, 1023, 1086, 1205, 1779
DpnII GATC 8 cut(s) 81, 244, 553, 1012, 1021, 1084, 1203, 1777
EaeI YGGCCR 1 cut(s) 1446
Eam1104I CTCTTC 5 cut(s) 6, 537, 789, 1116, 1850
EarI CTCTTC 5 cut(s) 6, 537, 789, 1116, 1850
EciI GGCGGA 1 cut(s) 47
Eco130I CCWWGG 2 cut(s) 999, 1850
Eco147I AGGCCT 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 770
Eco31I GGTCTC 1 cut(s) 1148
Eco47I GGWCC 2 cut(s) 1298, 1937
Eco57I CTGAAG 1 cut(s) 1490
Eco81I CCTNAGG 1 cut(s) 889
Eco88I CYCGRG 1 cut(s) 1341
Eco91I GGTNACC 1 cut(s) 1854
EcoO109I RGGNCCY 2 cut(s) 97, 1298
EcoO65I GGTNACC 1 cut(s) 1854
EcoRI GAATTC 1 cut(s) 968
EcoRII CCWGG 3 cut(s) 1300, 1708, 1973
EcoT14I CCWWGG 2 cut(s) 999, 1850
EcoT38I GRGCYC 1 cut(s) 770
ErhI CCWWGG 2 cut(s) 999, 1850
FalI AAGNNNNNCTT 4 cut(s) 719, 751, 1213, 1245
FaqI GGGAC 3 cut(s) 22, 166, 1311
FbaI TGATCA 1 cut(s) 1203
Fnu4HI GCNGC 6 cut(s) 403, 665, 784, 1238, 1381, 1888
FokI GGATG 6 cut(s) 293, 668, 881, 945, 1259, 1996
FriOI GRGCYC 1 cut(s) 770
Fsp4HI GCNGC 6 cut(s) 403, 665, 784, 1238, 1381, 1888
FspBI CTAG 3 cut(s) 1088, 1190, 1695
GluI GCNGC 6 cut(s) 403, 665, 784, 1238, 1381, 1888
GsaI CCCAGC 2 cut(s) 53, 1806
GsuI CTGGAG 4 cut(s) 1262, 1437, 1731, 1957
HaeIII GGCC 4 cut(s) 98, 126, 386, 1448
HapII CCGG 3 cut(s) 150, 557, 685
HpaII CCGG 3 cut(s) 150, 557, 685
HphI GGTGA 1 cut(s) 650
Hpy166II GTNNAC 1 cut(s) 1252
Hpy188I TCNGA 4 cut(s) 226, 244, 280, 1768
Hpy188III TCNNGA 9 cut(s) 91, 883, 904, 973, 1062, 1130, 1221, 1397, 1416
Hpy8I GTNNAC 1 cut(s) 1252
HpyAV CCTTC 8 cut(s) 88, 349, 375, 459, 542, 745, 1712, 1950
HpyCH4III ACNGT 2 cut(s) 708, 1101
HpyCH4IV ACGT 1 cut(s) 1991
HpyF10VI GCNNNNNNNGC 2 cut(s) 1065, 1418
HpyF3I CTNAG 5 cut(s) 167, 599, 889, 944, 1364
HpySE526I ACGT 1 cut(s) 1991
Ksp22I TGATCA 1 cut(s) 1203
KspI CCGCGG 1 cut(s) 406
Kzo9I GATC 8 cut(s) 81, 244, 553, 1012, 1021, 1084, 1203, 1777
LmnI GCTCC 6 cut(s) 399, 445, 975, 1064, 1418, 1976
Lsp1109I GCAGC 5 cut(s) 651, 770, 1249, 1367, 1899
LweI GCATC 7 cut(s) 481, 795, 834, 923, 930, 1133, 2018
MabI ACCWGGT 1 cut(s) 1300
MaeI CTAG 3 cut(s) 1088, 1190, 1695
MaeII ACGT 1 cut(s) 1991
MaeIII GTNAC 3 cut(s) 638, 1854, 1946
MalI GATC 8 cut(s) 83, 246, 555, 1014, 1023, 1086, 1205, 1779
MboI GATC 8 cut(s) 81, 244, 553, 1012, 1021, 1084, 1203, 1777
MflI RGATCY 2 cut(s) 553, 1012
MhlI GDGCHC 3 cut(s) 770, 1254, 1338
MlsI TGGCCA 1 cut(s) 1448
MluNI TGGCCA 1 cut(s) 1448
MlyI GAGTC 7 cut(s) 44, 269, 909, 1142, 1190, 1211, 1836
MmeI TCCRAC 3 cut(s) 505, 1724, 1840
Mox20I TGGCCA 1 cut(s) 1448
MscI TGGCCA 1 cut(s) 1448
MseI TTAA 7 cut(s) 249, 315, 503, 722, 1151, 1452, 1587
Msp20I TGGCCA 1 cut(s) 1448
MspA1I CMGCKG 2 cut(s) 405, 1240
MspI CCGG 3 cut(s) 150, 557, 685
MspR9I CCNGG 5 cut(s) 151, 558, 1302, 1710, 1975
Mva1269I GAATGC 1 cut(s) 1911
MvaI CCWGG 3 cut(s) 1302, 1710, 1975
MvnI CGCG 2 cut(s) 405, 1131
MwoI GCNNNNNNNGC 2 cut(s) 1065, 1418
NciI CCSGG 2 cut(s) 151, 558
NdeII GATC 8 cut(s) 81, 244, 553, 1012, 1021, 1084, 1203, 1777
NlaIV GGNNCC 8 cut(s) 401, 447, 555, 895, 997, 1060, 1074, 1299
NmuCI GTSAC 2 cut(s) 638, 1946
NruI TCGCGA 1 cut(s) 1131
NspI RCATGY 3 cut(s) 1213, 1707, 1790
NspV TTCGAA 1 cut(s) 379
PagI TCATGA 2 cut(s) 90, 1396
PceI AGGCCT 1 cut(s) 126
PctI GAATGC 1 cut(s) 1911
PfeI GAWTC 3 cut(s) 239, 907, 1224
PflMI CCANNNNNTGG 1 cut(s) 1055
PfoI TCCNGGA 1 cut(s) 1973
PkrI GCNGC 6 cut(s) 404, 666, 785, 1239, 1382, 1889
PleI GAGTC 7 cut(s) 43, 269, 908, 1141, 1190, 1211, 1836
PpsI GAGTC 7 cut(s) 43, 269, 908, 1141, 1190, 1211, 1836
PpuMI RGGWCCY 1 cut(s) 1298
PshBI ATTAAT 1 cut(s) 1452
PsiI TTATAA 1 cut(s) 1682
Psp5II RGGWCCY 1 cut(s) 1298
Psp6I CCWGG 3 cut(s) 1300, 1708, 1973
PspEI GGTNACC 1 cut(s) 1854
PspFI CCCAGC 2 cut(s) 49, 1802
PspGI CCWGG 3 cut(s) 1300, 1708, 1973
PspN4I GGNNCC 8 cut(s) 401, 447, 555, 895, 997, 1060, 1074, 1299
PspPI GGNCC 4 cut(s) 97, 384, 1298, 1937
PspPPI RGGWCCY 1 cut(s) 1298
PstI CTGCAG 1 cut(s) 669
PstNI CAGNNNCTG 1 cut(s) 1082
PsuI RGATCY 2 cut(s) 553, 1012
PvuII CAGCTG 1 cut(s) 1240
RruI TCGCGA 1 cut(s) 1131
RsaI GTAC 4 cut(s) 62, 960, 1271, 1797
RsaNI GTAC 4 cut(s) 61, 959, 1270, 1796
SacII CCGCGG 1 cut(s) 406
SaqAI TTAA 7 cut(s) 249, 315, 503, 722, 1151, 1452, 1587
SatI GCNGC 6 cut(s) 403, 665, 784, 1238, 1381, 1888
Sau3AI GATC 8 cut(s) 81, 244, 553, 1012, 1021, 1084, 1203, 1777
Sau96I GGNCC 4 cut(s) 97, 384, 1298, 1937
ScaI AGTACT 1 cut(s) 1271
SchI GAGTC 7 cut(s) 44, 269, 909, 1142, 1190, 1211, 1836
ScrFI CCNGG 5 cut(s) 151, 558, 1302, 1710, 1975
SduI GDGCHC 3 cut(s) 770, 1254, 1338
SexAI ACCWGGT 1 cut(s) 1300
SfaNI GCATC 7 cut(s) 481, 795, 834, 923, 930, 1133, 2018
SfcI CTRYAG 3 cut(s) 665, 1821, 1966
Sfr303I CCGCGG 1 cut(s) 406
SfuI TTCGAA 1 cut(s) 379
SgrBI CCGCGG 1 cut(s) 406
SinI GGWCC 2 cut(s) 1298, 1937
SmlI CTYRAG 3 cut(s) 769, 1145, 1219
SmoI CTYRAG 3 cut(s) 769, 1145, 1219
SpeI ACTAGT 1 cut(s) 1694
SseBI AGGCCT 1 cut(s) 126
SsiI CCGC 6 cut(s) 32, 403, 405, 453, 1383, 1593
SspI AATATT 2 cut(s) 1162, 1759
SspMI CTAG 3 cut(s) 1088, 1190, 1695
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 5 cut(s) 149, 556, 1300, 1708, 1973
StyI CCWWGG 2 cut(s) 999, 1850
TaaI ACNGT 2 cut(s) 708, 1101
TaiI ACGT 1 cut(s) 1994
TaqI TCGA 4 cut(s) 84, 138, 268, 379
TatI WGTACW 3 cut(s) 958, 1269, 1795
TauI GCSGC 1 cut(s) 405
TfiI GAWTC 3 cut(s) 239, 907, 1224
Tru1I TTAA 7 cut(s) 249, 315, 503, 722, 1151, 1452, 1587
Tru9I TTAA 7 cut(s) 249, 315, 503, 722, 1151, 1452, 1587
TscAI CASTG 4 cut(s) 1259, 1549, 1656, 1866
TseFI GTSAC 2 cut(s) 638, 1946
TseI GCWGC 5 cut(s) 664, 783, 1237, 1380, 1887
Tsp45I GTSAC 2 cut(s) 638, 1946
TspGWI ACGGA 2 cut(s) 48, 2002
TspRI CASTG 4 cut(s) 1259, 1549, 1656, 1866
Van91I CCANNNNNTGG 1 cut(s) 1055
VneI GTGCAC 1 cut(s) 1250
VpaK11BI GGWCC 2 cut(s) 1298, 1937
VspI ATTAAT 1 cut(s) 1452
XapI RAATTY 7 cut(s) 44, 362, 472, 647, 968, 1386, 1636
XceI RCATGY 3 cut(s) 1213, 1707, 1790
XspI CTAG 3 cut(s) 1088, 1190, 1695
ZrmI AGTACT 1 cut(s) 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.