pycom10g00390

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008617
Physical Location & Seq
Forward (+)
440427 .. 446431
6005 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1008 bp
ATGCGCACCCTCCTCACTCTTAGGCCACCACCAGCACCACCATCTTCGTCGTGTCTTCTCCTCCCATGCCTCTCCGCGCTCTCTCCTCCTCCCTTCTCCTCCCACCTCTTCAGCTTTCGACCCAACAAACGCTTCCACTTCCTCACCCCGTGCTCCTCTCTCAAGCAGTCCAAGAAGAAGTCCCTCCAGAACACCCCTAATGCTCCTCCGCAGAGCCAGCGCTGGTTCTTCAACATCCCCAAGAGCGACGACGCCGGCGATATTGAAAAGACAGGCGAGGATGAAGACGGTGGTTTCGACGGCGACAATGCGCTTAAAGGCACCGTTTTGGCCGGTGTTTTCCTGGTCGGCATTGTAGGTGGGCTCGCTGGTGTTGGGTACGTCTACAAGGACCAGATCAACGCCTTCTTGAATCAGTTTTCCACTCTCATTGAAGGTTATGGTCCAGCTGGATATGCTTTGTTTGTAGCAGTTTATGCCGGACTGGAAATCATTGCGATTCCAGCAATTCCATTGACCATGTCAGCTGGTCTTCTCTTTGGCACAGTGACTGGCACTATTCTCGTCTCCATAAGTGGCACGGTGGCTGCTAGCATTGCCTTTTTGATTGCTAGATATTTTGCTCGTGAGCGTATTCTGAAAATGGTTGAGGGAAACAAAACGTTTCTTGCCATTGATAAGGCTATTGGAGAAAATGGATTTAGAGTTGTCACTCTCCTTCGCTTGAGCCCTTTGCTTCCTTTTTCGTTGGGGAACTATTTGTATGGATTGACATCTGTAAAGTTTGTACCATACGTGTTGGGAAGTTGGTTGGGGATGCTTCCAGGAACATGGGCTTATGTGAGTGCTGGTGCATTTGGGCGAGCTATAATTCAAGAAGAATCTGAGGTTGGTTTGCCAGGAGGAAATGGTCAGCTCTTGACACTAGGAGTTGGACTGTTAGCCACAGCATTGGCTGCTGTTTATGTAACACGACTGGCAAAGGATGCTGTAAAAGATATCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

336

Amino Acids

35.6

Weight (kDa)

8.86

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 167 - 285 4.4e-23 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 320
AccI GTMKAC 1 cut(s) 384
AccII CGCG 1 cut(s) 77
AciI CCGC 2 cut(s) 75, 209
AclI AACGTT 1 cut(s) 662
AcoI YGGCCR 1 cut(s) 330
AcuI CTGAAG 1 cut(s) 94
AcyI GRCGYC 1 cut(s) 252
AdeI CACNNNGTG 1 cut(s) 150
AfaI GTAC 2 cut(s) 380, 789
AfeI AGCGCT 1 cut(s) 221
AflIII ACRYGT 1 cut(s) 795
AgsI TTSAA 5 cut(s) 232, 266, 412, 434, 875
AjnI CCWGG 3 cut(s) 342, 823, 898
AjuI GAANNNNNNNTTGG 4 cut(s) 116, 148, 873, 905
AluBI AGCT 5 cut(s) 114, 449, 527, 866, 916
AluI AGCT 5 cut(s) 114, 449, 527, 866, 916
Alw21I GWGCWC 1 cut(s) 155
Alw26I GTCTC 1 cut(s) 571
AlwNI CAGNNNCTG 1 cut(s) 551
Aor51HI AGCGCT 1 cut(s) 221
AoxI GGCC 2 cut(s) 23, 330
ApeKI GCWGC 2 cut(s) 587, 956
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AspLEI GCGC 4 cut(s) 6, 79, 222, 313
AspS9I GGNCC 2 cut(s) 391, 443
AsuHPI GGTGA 1 cut(s) 136
AsuNHI GCTAGC 1 cut(s) 590
AvaII GGWCC 2 cut(s) 391, 443
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 2 cut(s) 366, 731
BauI CACGAG 1 cut(s) 624
BbsI GAAGAC 3 cut(s) 47, 291, 524
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 2 cut(s) 574, 943
BccI CCATC 1 cut(s) 49
BceAI ACGGC 1 cut(s) 316
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 3 cut(s) 344, 825, 900
BcoDI GTCTC 1 cut(s) 571
BfaI CTAG 3 cut(s) 591, 612, 926
BfoI RGCGCY 1 cut(s) 223
BisI GCNGC 2 cut(s) 588, 957
BlsI GCNGC 2 cut(s) 589, 958
Bme1390I CCNGG 3 cut(s) 344, 825, 900
Bme18I GGWCC 2 cut(s) 391, 443
BmgT120I GGNCC 2 cut(s) 391, 443
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 3 cut(s) 344, 825, 900
BmsI GCATC 2 cut(s) 807, 976
BmtI GCTAGC 1 cut(s) 594
BpiI GAAGAC 3 cut(s) 47, 291, 524
BpmI CTGGAG 1 cut(s) 170
BpuEI CTTGAG 2 cut(s) 146, 745
Bsa29I ATCGAT 1 cut(s) 1002
BsaAI YACGTR 1 cut(s) 796
BsaBI GATNNNNATC 1 cut(s) 772
BsaHI GRCGYC 1 cut(s) 252
Bse118I RCCGGY 2 cut(s) 254, 332
Bse1I ACTGG 3 cut(s) 489, 556, 981
Bse3DI GCAATG 2 cut(s) 492, 594
Bse8I GATNNNNATC 1 cut(s) 772
BseBI CCWGG 3 cut(s) 344, 825, 900
BseCI ATCGAT 1 cut(s) 1002
BseGI GGATG 4 cut(s) 234, 286, 822, 991
BseJI GATNNNNATC 1 cut(s) 772
BseMI GCAATG 2 cut(s) 492, 594
BseMII CTCAG 1 cut(s) 876
BseNI ACTGG 3 cut(s) 489, 556, 981
BseRI GAGGAG 6 cut(s) 50, 75, 78, 88, 145, 195
BseXI GCAGC 2 cut(s) 574, 943
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 2 cut(s) 25, 332
BshNI GGYRCC 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 1002
BsiHKAI GWGCWC 1 cut(s) 155
BsiSI CCGG 3 cut(s) 255, 333, 480
BslFI GGGAC 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 571
BsmBI CGTCTC 1 cut(s) 571
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 2 cut(s) 25, 332
Bsp1286I GDGCHC 3 cut(s) 155, 366, 731
Bsp143I GATC 1 cut(s) 396
BspACI CCGC 2 cut(s) 75, 209
BspANI GGCC 2 cut(s) 25, 332
BspCNI CTCAG 1 cut(s) 877
BspDI ATCGAT 1 cut(s) 1002
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 1 cut(s) 322
BspOI GCTAGC 1 cut(s) 594
BspT107I GGYRCC 1 cut(s) 320
BsrDI GCAATG 2 cut(s) 492, 594
BsrFI RCCGGY 2 cut(s) 254, 332
BsrI ACTGG 3 cut(s) 489, 556, 981
BssAI RCCGGY 2 cut(s) 254, 332
BssMI GATC 1 cut(s) 396
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 624
Bst2BI CACGAG 1 cut(s) 624
Bst2UI CCWGG 3 cut(s) 344, 825, 900
Bst4CI ACNGT 5 cut(s) 290, 325, 547, 583, 939
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 252
BstAPI GCANNNNNTGC 3 cut(s) 476, 956, 986
BstBAI YACGTR 1 cut(s) 796
BstC8I GCNNGC 5 cut(s) 218, 256, 366, 592, 864
BstDEI CTNAG 2 cut(s) 20, 885
BstF5I GGATG 4 cut(s) 234, 286, 822, 991
BstFNI CGCG 1 cut(s) 77
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 4 cut(s) 6, 79, 222, 313
BstKTI GATC 1 cut(s) 399
BstMAI GTCTC 1 cut(s) 571
BstMBI GATC 1 cut(s) 396
BstMWI GCNNNNNNNGC 7 cut(s) 217, 455, 476, 503, 596, 956, 986
BstNI CCWGG 3 cut(s) 344, 825, 900
BstSCI CCNGG 3 cut(s) 342, 823, 898
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 574, 943
BstV2I GAAGAC 3 cut(s) 47, 291, 524
BstXI CCANNNNNNTGG 2 cut(s) 831, 952
Bsu15I ATCGAT 1 cut(s) 1002
BsuRI GGCC 2 cut(s) 25, 332
BsuTUI ATCGAT 1 cut(s) 1002
BtsCI GGATG 4 cut(s) 234, 286, 822, 991
BtsIMutI CAGTG 1 cut(s) 552
Cac8I GCNNGC 5 cut(s) 218, 256, 366, 592, 864
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 4 cut(s) 6, 79, 222, 313
Cfr10I RCCGGY 2 cut(s) 254, 332
Cfr13I GGNCC 2 cut(s) 391, 443
ClaI ATCGAT 1 cut(s) 1002
CseI GACGC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 379, 788
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 3 cut(s) 66, 520, 831
CviQI GTAC 2 cut(s) 379, 788
DdeI CTNAG 2 cut(s) 20, 885
DpnI GATC 1 cut(s) 398
DpnII GATC 1 cut(s) 396
DraIII CACNNNGTG 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 330
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco24I GRGCYC 2 cut(s) 366, 731
Eco32I GATATC 1 cut(s) 1000
Eco47I GGWCC 2 cut(s) 391, 443
Eco47III AGCGCT 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 94
EcoRII CCWGG 3 cut(s) 342, 823, 898
EcoRV GATATC 1 cut(s) 1000
EcoT38I GRGCYC 2 cut(s) 366, 731
Esp3I CGTCTC 1 cut(s) 571
FaeI CATG 3 cut(s) 69, 523, 834
FaqI GGGAC 1 cut(s) 166
FatI CATG 3 cut(s) 65, 519, 830
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 2 cut(s) 588, 957
FokI GGATG 4 cut(s) 221, 293, 829, 998
FriOI GRGCYC 2 cut(s) 366, 731
Fsp4HI GCNGC 2 cut(s) 588, 957
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 3 cut(s) 591, 612, 926
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 4 cut(s) 5, 78, 221, 312
GluI GCNGC 2 cut(s) 588, 957
GsuI CTGGAG 1 cut(s) 170
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 2 cut(s) 25, 332
HapII CCGG 3 cut(s) 255, 333, 480
HgaI GACGC 1 cut(s) 260
HhaI GCGC 4 cut(s) 6, 79, 222, 313
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 3 cut(s) 69, 523, 834
Hin6I GCGC 4 cut(s) 4, 77, 220, 311
HinP1I GCGC 4 cut(s) 4, 77, 220, 311
HinfI GANTC 3 cut(s) 412, 499, 881
HpaII CCGG 3 cut(s) 255, 333, 480
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 2 cut(s) 639, 886
Hpy188III TCNNGA 5 cut(s) 187, 409, 626, 875, 919
Hpy8I GTNNAC 1 cut(s) 385
Hpy99I CGWCG 4 cut(s) 52, 251, 254, 302
HpyAV CCTTC 4 cut(s) 103, 415, 428, 728
HpyCH4III ACNGT 5 cut(s) 290, 325, 547, 583, 939
HpyCH4IV ACGT 3 cut(s) 381, 662, 795
HpyCH4V TGCA 1 cut(s) 854
HpyF10VI GCNNNNNNNGC 7 cut(s) 217, 455, 476, 503, 596, 956, 986
HpyF3I CTNAG 2 cut(s) 20, 885
HpySE526I ACGT 3 cut(s) 381, 662, 795
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 3 cut(s) 69, 523, 834
HspAI GCGC 4 cut(s) 4, 77, 220, 311
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Kzo9I GATC 1 cut(s) 396
LmnI GCTCC 2 cut(s) 158, 208
Lsp1109I GCAGC 2 cut(s) 574, 943
LweI GCATC 2 cut(s) 807, 976
MaeI CTAG 3 cut(s) 591, 612, 926
MaeII ACGT 3 cut(s) 381, 662, 795
MaeIII GTNAC 3 cut(s) 547, 709, 967
MalI GATC 1 cut(s) 398
MboI GATC 1 cut(s) 396
MboII GAAGA 8 cut(s) 36, 47, 100, 187, 220, 296, 524, 890
MhlI GDGCHC 3 cut(s) 155, 366, 731
MluCI AATT 2 cut(s) 507, 870
MmeI TCCRAC 1 cut(s) 913
MreI CGCCGGCG 1 cut(s) 254
MroNI GCCGGC 1 cut(s) 254
MseI TTAA 1 cut(s) 315
MspA1I CMGCKG 2 cut(s) 449, 527
MspI CCGG 3 cut(s) 255, 333, 480
MspR9I CCNGG 3 cut(s) 344, 825, 900
MvaI CCWGG 3 cut(s) 344, 825, 900
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 7 cut(s) 217, 455, 476, 503, 596, 956, 986
NaeI GCCGGC 1 cut(s) 256
NdeII GATC 1 cut(s) 396
NgoMIV GCCGGC 1 cut(s) 254
NheI GCTAGC 1 cut(s) 590
NlaIII CATG 3 cut(s) 69, 523, 834
NlaIV GGNNCC 1 cut(s) 322
NmuCI GTSAC 2 cut(s) 547, 709
NsbI TGCGCA 1 cut(s) 5
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 294
PdiI GCCGGC 1 cut(s) 256
PfeI GAWTC 3 cut(s) 412, 499, 881
PflFI GACNNNGTC 1 cut(s) 520
PfoI TCCNGGA 1 cut(s) 823
PkrI GCNGC 2 cut(s) 589, 958
Ppu21I YACGTR 1 cut(s) 796
Psp1406I AACGTT 1 cut(s) 662
Psp6I CCWGG 3 cut(s) 342, 823, 898
PspGI CCWGG 3 cut(s) 342, 823, 898
PspN4I GGNNCC 1 cut(s) 322
PspPI GGNCC 2 cut(s) 391, 443
PstNI CAGNNNCTG 1 cut(s) 551
PsyI GACNNNGTC 1 cut(s) 520
PvuII CAGCTG 2 cut(s) 449, 527
RsaI GTAC 2 cut(s) 380, 789
RsaNI GTAC 2 cut(s) 379, 788
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 2 cut(s) 588, 957
Sau3AI GATC 1 cut(s) 396
Sau96I GGNCC 2 cut(s) 391, 443
ScrFI CCNGG 3 cut(s) 344, 825, 900
SduI GDGCHC 3 cut(s) 155, 366, 731
SfaNI GCATC 2 cut(s) 807, 976
SgrAI CRCCGGYG 1 cut(s) 254
SinI GGWCC 2 cut(s) 391, 443
SmlI CTYRAG 2 cut(s) 161, 724
SmoI CTYRAG 2 cut(s) 161, 724
Sse9I AATT 2 cut(s) 507, 870
SsiI CCGC 2 cut(s) 75, 209
SspMI CTAG 3 cut(s) 591, 612, 926
StyD4I CCNGG 3 cut(s) 342, 823, 898
TaaI ACNGT 5 cut(s) 290, 325, 547, 583, 939
TaiI ACGT 3 cut(s) 384, 665, 798
TaqI TCGA 3 cut(s) 118, 297, 1002
TasI AATT 2 cut(s) 507, 870
TfiI GAWTC 3 cut(s) 412, 499, 881
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 1 cut(s) 552
TseFI GTSAC 2 cut(s) 547, 709
TseI GCWGC 2 cut(s) 587, 956
Tsp45I GTSAC 2 cut(s) 547, 709
TspDTI ATGAA 1 cut(s) 297
TspRI CASTG 1 cut(s) 552
Tth111I GACNNNGTC 1 cut(s) 520
VpaK11BI GGWCC 2 cut(s) 391, 443
XmiI GTMKAC 1 cut(s) 384
XspI CTAG 3 cut(s) 591, 612, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.