pycom10g00430

Succinate dehydrogenase assembly factor 1 homolog, mitochondrial

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003139
Physical Location & Seq
Forward (+)
481623 .. 481922
300 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00430.1

Sequence Viewer

Length: 300 bp
ATGGGACCCTCCAGTGCACCAAGGCGGCTTTCTGGGATGCAGAAGCAAGTACTAAGTCTATACAGAGGGTTTCTGAGGGCAGCCCGCACAAAATCTGCTGAAGACCGGCAGCGCATTGAGTCACTTGTATCAATTGAGTTCCGCCGCAATGCAAAGGAAGTAGACCGCAAAAATTTCCTCTACATTGAGTACTTGCTGCGACGTGGTAAGAAACAGCTTGATCAGCTCAGGAGCCCCGATATTGTTGGATTATCAGCTACAAATGTCAGTTTCTCTCAAACGAAGGATCCAACACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Complex1_LYR

Protein Analysis

100

Amino Acids

11.41

Weight (kDa)

10.71

Isoelectric Point (pI)

59.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_LYR PF05347 15 - 75 5.3e-12 Complex 1 protein (LYR family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 162
AciI CCGC 5 cut(s) 25, 85, 142, 145, 166
AclWI GGATC 2 cut(s) 281, 294
AcsI RAATTY 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 120
AfaI GTAC 2 cut(s) 51, 191
AjiI CACGTC 1 cut(s) 203
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AluBI AGCT 3 cut(s) 217, 226, 257
AluI AGCT 3 cut(s) 217, 226, 257
Alw21I GWGCWC 1 cut(s) 19
Alw44I GTGCAC 1 cut(s) 15
AlwI GGATC 2 cut(s) 281, 294
ApaLI GTGCAC 1 cut(s) 15
ApeKI GCWGC 3 cut(s) 80, 109, 196
ApoI RAATTY 1 cut(s) 172
AspLEI GCGC 1 cut(s) 114
AspS9I GGNCC 1 cut(s) 5
AvaII GGWCC 1 cut(s) 5
BaeGI GKGCMC 1 cut(s) 19
BamHI GGATCC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 92, 121, 183
BclI TGATCA 1 cut(s) 220
BisI GCNGC 5 cut(s) 26, 81, 110, 145, 197
BlsI GCNGC 5 cut(s) 27, 82, 111, 146, 198
BmcAI AGTACT 2 cut(s) 51, 191
Bme18I GGWCC 1 cut(s) 5
BmgBI CACGTC 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 5
BmiI GGNNCC 4 cut(s) 6, 7, 233, 288
BmsI GCATC 1 cut(s) 27
BpiI GAAGAC 1 cut(s) 108
Bpu10I CCTNAGC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 20
Bse118I RCCGGY 1 cut(s) 105
Bse1I ACTGG 1 cut(s) 12
Bse3DI GCAATG 1 cut(s) 154
BseDI CCNNGG 1 cut(s) 20
BseGI GGATG 1 cut(s) 42
BseMI GCAATG 1 cut(s) 154
BseMII CTCAG 2 cut(s) 65, 241
BseNI ACTGG 1 cut(s) 12
BseSI GKGCMC 1 cut(s) 19
BseXI GCAGC 3 cut(s) 92, 121, 183
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 1 cut(s) 106
BslFI GGGAC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 19, 236
Bsp143I GATC 2 cut(s) 220, 286
BspACI CCGC 5 cut(s) 25, 85, 142, 145, 166
BspCNI CTCAG 2 cut(s) 66, 240
BspLI GGNNCC 4 cut(s) 6, 7, 233, 288
BspPI GGATC 2 cut(s) 281, 294
BsrDI GCAATG 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 105
BsrI ACTGG 1 cut(s) 12
BssAI RCCGGY 1 cut(s) 105
BssECI CCNNGG 1 cut(s) 20
BssMI GATC 2 cut(s) 220, 286
BssT1I CCWWGG 1 cut(s) 20
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 3 cut(s) 53, 74, 227
BstF5I GGATG 1 cut(s) 42
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 2 cut(s) 223, 289
BstMBI GATC 2 cut(s) 220, 286
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 19
BstV1I GCAGC 3 cut(s) 92, 121, 183
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BtrI CACGTC 1 cut(s) 203
BtsCI GGATG 1 cut(s) 42
BtsIMutI CAGTG 2 cut(s) 19, 295
Cac8I GCNNGC 1 cut(s) 85
CfoI GCGC 1 cut(s) 114
Cfr10I RCCGGY 1 cut(s) 105
Cfr13I GGNCC 1 cut(s) 5
Csp6I GTAC 2 cut(s) 50, 190
CviJI RGCY 6 cut(s) 28, 83, 217, 226, 234, 257
CviKI_1 RGCY 6 cut(s) 28, 83, 217, 226, 234, 257
CviQI GTAC 2 cut(s) 50, 190
DdeI CTNAG 3 cut(s) 53, 74, 227
DpnI GATC 2 cut(s) 222, 288
DpnII GATC 2 cut(s) 220, 286
EciI GGCGGA 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 5
Eco57I CTGAAG 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 20
EcoT38I GRGCYC 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 20
FaiI YATR 1 cut(s) 61
FaqI GGGAC 1 cut(s) 18
FauI CCCGC 1 cut(s) 92
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 5 cut(s) 26, 81, 110, 145, 197
FokI GGATG 1 cut(s) 49
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 5 cut(s) 26, 81, 110, 145, 197
GlaI GCGC 1 cut(s) 113
GluI GCNGC 5 cut(s) 26, 81, 110, 145, 197
HapII CCGG 1 cut(s) 106
HhaI GCGC 1 cut(s) 114
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HinfI GANTC 1 cut(s) 119
HpaII CCGG 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 17, 163
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 2 cut(s) 17, 163
Hpy99I CGWCG 1 cut(s) 204
HpyAV CCTTC 1 cut(s) 277
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 3 cut(s) 17, 40, 152
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpyF3I CTNAG 3 cut(s) 53, 74, 227
HpySE526I ACGT 1 cut(s) 202
HspAI GCGC 1 cut(s) 112
KflI GGGWCCC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 2 cut(s) 220, 286
LmnI GCTCC 1 cut(s) 231
LpnPI CCDG 4 cut(s) 18, 25, 119, 214
Lsp1109I GCAGC 3 cut(s) 92, 121, 183
LweI GCATC 1 cut(s) 27
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 1 cut(s) 120
MalI GATC 2 cut(s) 222, 288
MboI GATC 2 cut(s) 220, 286
MboII GAAGA 1 cut(s) 113
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 2 cut(s) 19, 236
MluCI AATT 2 cut(s) 132, 172
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 4 cut(s) 19, 59, 69, 188
MspI CCGG 1 cut(s) 106
MunI CAATTG 1 cut(s) 132
MwoI GCNNNNNNNGC 1 cut(s) 223
NdeII GATC 2 cut(s) 220, 286
NlaIV GGNNCC 4 cut(s) 6, 7, 233, 288
NmuCI GTSAC 1 cut(s) 120
PkrI GCNGC 5 cut(s) 27, 82, 111, 146, 198
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PpuMI RGGWCCY 1 cut(s) 5
Psp5II RGGWCCY 1 cut(s) 5
PspN4I GGNNCC 4 cut(s) 6, 7, 233, 288
PspPI GGNCC 1 cut(s) 5
PspPPI RGGWCCY 1 cut(s) 5
PsuI RGATCY 1 cut(s) 286
RsaI GTAC 2 cut(s) 51, 191
RsaNI GTAC 2 cut(s) 50, 190
SatI GCNGC 5 cut(s) 26, 81, 110, 145, 197
Sau3AI GATC 2 cut(s) 220, 286
Sau96I GGNCC 1 cut(s) 5
ScaI AGTACT 2 cut(s) 51, 191
SchI GAGTC 1 cut(s) 128
SduI GDGCHC 2 cut(s) 19, 236
SetI ASST 4 cut(s) 205, 219, 228, 259
SfaNI GCATC 1 cut(s) 27
SinI GGWCC 1 cut(s) 5
Sse9I AATT 2 cut(s) 132, 172
SsiI CCGC 5 cut(s) 25, 85, 142, 145, 166
StyI CCWWGG 1 cut(s) 20
TaiI ACGT 1 cut(s) 205
TasI AATT 2 cut(s) 132, 172
TatI WGTACW 2 cut(s) 49, 189
TauI GCSGC 2 cut(s) 28, 147
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 120
TseI GCWGC 3 cut(s) 80, 109, 196
Tsp45I GTSAC 1 cut(s) 120
TspRI CASTG 1 cut(s) 19
VneI GTGCAC 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 5
XapI RAATTY 1 cut(s) 172
XmiI GTMKAC 1 cut(s) 162
ZrmI AGTACT 2 cut(s) 51, 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.