pycom10g04320
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011645
Physical Location & Seq
Forward (+)
4560822 .. 4570716
9895 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 840 bp
ATGGTCCATACCATGTTTCCACAAAAAAACAGCAGCGGAGGAGGTTGCAACTGTTTTCTCCATAGCTTGTTTAATCTTCTAAAAGGTATGGGGAGAGGAAAAATTGTGATTCGAAGGATTGACAATTCGACGAGCAGGCAAGTGACCTTTTCGAAGAGAAGAAATGGGTTGCTTAAGAAGGCTAAGGAGCTATCAATCCTTTGTGATGCTGAGGTTGGACTGATTATCTTCTCTAGCACCGGCAGGCTTTACGATTATGCTAGCACTAGCATGCAATCAGTTATCGATCGCTTCAACAAACTGAAAGCTGAGCATCATCGACTGCTGAATCCTGCTGCAGAAGTCAAGTTTTGGCAAAGGGAGGCAGCAAGCTTGAGGCAACAATTGCAGTACTTGGAAGAATGCCACAGGCAATTAATGGGGGAAGAACTTTCTGGTTTGAGTGCCAAAGATCTACAGAATTTGGAAAGCCAGCTGGAGATGAGTTTGAAAGGTGTCCGGACGAAAAAGAACCAGATATTTAATGATGAAATAAAAGAACTGAGCCAGAAGGAAATTTTCATACATCAAGAAAATATAGAACTGTATAAGAAGCTAAACCTCATAAGTAAAGAGAATGAGGAACTGCAAAACAAGGTTTATGAAGCAAGGGATGTGAATCGAGGAAACAAAAGTTCCCAGCCTCCATATAGCATCAGTAATGATTATGATTTGCATGCACCCATCCATCTGCAGCTAAGCCAGCCACAGAATCAAACTCAGCCTGGGCCTGGTCCTAAGAACAATGAAGTACCAGCAAAAGCAATATATAACCCCACTAAAGAATTACAACTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

280

Amino Acids

31.93

Weight (kDa)

9.21

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 39 - 86 1.3e-25 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 113 - 199 3.7e-27 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 498
AciI CCGC 1 cut(s) 36
AcsI RAATTY 2 cut(s) 460, 555
AfaI GTAC 2 cut(s) 392, 792
AfiI CCNNNNNNNGG 1 cut(s) 770
AflII CTTAAG 1 cut(s) 173
AgsI TTSAA 2 cut(s) 295, 490
AjnI CCWGG 2 cut(s) 763, 769
AluBI AGCT 7 cut(s) 66, 190, 308, 372, 475, 595, 736
AluI AGCT 7 cut(s) 66, 190, 308, 372, 475, 595, 736
Aor13HI TCCGGA 1 cut(s) 498
AoxI GGCC 1 cut(s) 767
ApeKI GCWGC 4 cut(s) 33, 335, 365, 733
ApoI RAATTY 2 cut(s) 460, 555
AseI ATTAAT 1 cut(s) 416
AspS9I GGNCC 3 cut(s) 4, 767, 773
AsuII TTCGAA 2 cut(s) 112, 152
AsuNHI GCTAGC 1 cut(s) 260
AvaII GGWCC 2 cut(s) 4, 773
BbvCI CCTCAGC 1 cut(s) 210
BbvI GCAGC 4 cut(s) 45, 322, 377, 745
BccI CCATC 2 cut(s) 731, 735
BciT130I CCWGG 2 cut(s) 765, 771
BfaI CTAG 3 cut(s) 234, 261, 267
BfmI CTRYAG 3 cut(s) 336, 455, 731
BfrI CTTAAG 1 cut(s) 173
BglII AGATCT 1 cut(s) 451
BisI GCNGC 4 cut(s) 34, 336, 366, 734
BlpI GCTNAGC 2 cut(s) 309, 737
BlsI GCNGC 4 cut(s) 35, 337, 367, 735
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 2 cut(s) 765, 771
Bme18I GGWCC 2 cut(s) 4, 773
BmgT120I GGNCC 3 cut(s) 4, 767, 773
BmrFI CCNGG 2 cut(s) 765, 771
BmsI GCATC 3 cut(s) 196, 322, 702
BmtI GCTAGC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 497
Bpu10I CCTNAGC 2 cut(s) 183, 210
Bpu1102I GCTNAGC 2 cut(s) 309, 737
Bpu14I TTCGAA 2 cut(s) 112, 152
BpuEI CTTGAG 1 cut(s) 394
Bsa29I ATCGAT 1 cut(s) 285
BsaBI GATNNNNATC 1 cut(s) 657
BsaJI CCNNGG 1 cut(s) 764
BsaWI WCCGGW 1 cut(s) 498
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse118I RCCGGY 1 cut(s) 239
Bse8I GATNNNNATC 1 cut(s) 657
BseAI TCCGGA 1 cut(s) 498
BseBI CCWGG 2 cut(s) 765, 771
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 764
BseGI GGATG 2 cut(s) 658, 723
BseJI GATNNNNATC 1 cut(s) 657
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMII CTCAG 4 cut(s) 201, 300, 533, 773
BseRI GAGGAG 1 cut(s) 54
BseXI GCAGC 4 cut(s) 45, 322, 377, 745
BseYI CCCAGC 1 cut(s) 678
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 1 cut(s) 769
BshVI ATCGAT 1 cut(s) 285
BsiEI CGRYCG 1 cut(s) 289
BsiSI CCGG 2 cut(s) 240, 499
BslI CCNNNNNNNGG 1 cut(s) 770
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 1 cut(s) 769
Bsp119I TTCGAA 2 cut(s) 112, 152
Bsp13I TCCGGA 1 cut(s) 498
Bsp143I GATC 2 cut(s) 286, 451
Bsp1720I GCTNAGC 2 cut(s) 309, 737
BspACI CCGC 1 cut(s) 36
BspANI GGCC 1 cut(s) 769
BspCNI CTCAG 4 cut(s) 202, 301, 534, 772
BspDI ATCGAT 1 cut(s) 285
BspEI TCCGGA 1 cut(s) 498
BspMAI CTGCAG 2 cut(s) 340, 735
BspOI GCTAGC 1 cut(s) 264
BspT104I TTCGAA 2 cut(s) 112, 152
BspTI CTTAAG 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 239
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 764
BssMI GATC 2 cut(s) 286, 451
Bst2UI CCWGG 2 cut(s) 765, 771
Bst4CI ACNGT 2 cut(s) 53, 585
Bst6I CTCTTC 1 cut(s) 149
BstAFI CTTAAG 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 385
BstBI TTCGAA 2 cut(s) 112, 152
BstC8I GCNNGC 8 cut(s) 137, 245, 262, 272, 370, 473, 717, 743
BstDEI CTNAG 7 cut(s) 183, 210, 309, 542, 737, 759, 777
BstF5I GGATG 2 cut(s) 658, 723
BstKTI GATC 2 cut(s) 289, 454
BstMBI GATC 2 cut(s) 286, 451
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 2 cut(s) 385, 742
BstNI CCWGG 2 cut(s) 765, 771
BstNSI RCATGY 2 cut(s) 274, 719
BstSCI CCNGG 2 cut(s) 763, 769
BstSFI CTRYAG 3 cut(s) 336, 455, 731
BstV1I GCAGC 4 cut(s) 45, 322, 377, 745
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
Bsu15I ATCGAT 1 cut(s) 285
BsuRI GGCC 1 cut(s) 769
BsuTUI ATCGAT 1 cut(s) 285
BtsCI GGATG 2 cut(s) 658, 723
Cac8I GCNNGC 8 cut(s) 137, 245, 262, 272, 370, 473, 717, 743
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 3 cut(s) 4, 767, 773
ClaI ATCGAT 1 cut(s) 285
Csp6I GTAC 2 cut(s) 391, 791
CviAII CATG 3 cut(s) 13, 271, 716
CviQI GTAC 2 cut(s) 391, 791
DdeI CTNAG 7 cut(s) 183, 210, 309, 542, 737, 759, 777
DpnI GATC 2 cut(s) 288, 453
DpnII GATC 2 cut(s) 286, 451
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Eco47I GGWCC 2 cut(s) 4, 773
EcoRII CCWGG 2 cut(s) 763, 769
FaeI CATG 3 cut(s) 16, 274, 719
FatI CATG 3 cut(s) 12, 270, 715
Fnu4HI GCNGC 4 cut(s) 34, 336, 366, 734
FokI GGATG 2 cut(s) 665, 710
Fsp4HI GCNGC 4 cut(s) 34, 336, 366, 734
FspBI CTAG 3 cut(s) 234, 261, 267
GluI GCNGC 4 cut(s) 34, 336, 366, 734
GsaI CCCAGC 1 cut(s) 682
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 1 cut(s) 769
HapII CCGG 2 cut(s) 240, 499
Hin1II CATG 3 cut(s) 16, 274, 719
HindIII AAGCTT 1 cut(s) 370
HinfI GANTC 4 cut(s) 109, 328, 658, 751
HpaII CCGG 2 cut(s) 240, 499
Hpy188III TCNNGA 2 cut(s) 499, 569
Hpy99I CGWCG 1 cut(s) 133
HpyAV CCTTC 3 cut(s) 108, 172, 544
HpyCH4III ACNGT 2 cut(s) 53, 585
HpyCH4V TGCA 9 cut(s) 48, 274, 338, 388, 628, 715, 719, 733, 835
HpyF10VI GCNNNNNNNGC 2 cut(s) 385, 742
HpyF3I CTNAG 7 cut(s) 183, 210, 309, 542, 737, 759, 777
Hsp92II CATG 3 cut(s) 16, 274, 719
Kpn2I TCCGGA 1 cut(s) 498
Kzo9I GATC 2 cut(s) 286, 451
LmnI GCTCC 1 cut(s) 187
Lsp1109I GCAGC 4 cut(s) 45, 322, 377, 745
LweI GCATC 3 cut(s) 196, 322, 702
MaeI CTAG 3 cut(s) 234, 261, 267
MaeIII GTNAC 1 cut(s) 142
MalI GATC 2 cut(s) 288, 453
MboI GATC 2 cut(s) 286, 451
MboII GAAGA 6 cut(s) 68, 166, 171, 220, 410, 437
MfeI CAATTG 1 cut(s) 383
MflI RGATCY 1 cut(s) 451
MluCI AATT 7 cut(s) 102, 124, 383, 413, 460, 555, 824
MmeI TCCRAC 1 cut(s) 196
MroI TCCGGA 1 cut(s) 498
MseI TTAA 4 cut(s) 72, 174, 416, 522
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 36, 475
MspCI CTTAAG 1 cut(s) 173
MspI CCGG 2 cut(s) 240, 499
MspR9I CCNGG 2 cut(s) 765, 771
MunI CAATTG 1 cut(s) 383
Mva1269I GAATGC 1 cut(s) 407
MvaI CCWGG 2 cut(s) 765, 771
MwoI GCNNNNNNNGC 2 cut(s) 385, 742
NdeII GATC 2 cut(s) 286, 451
NheI GCTAGC 1 cut(s) 260
NlaIII CATG 3 cut(s) 16, 274, 719
NmuCI GTSAC 1 cut(s) 142
NspI RCATGY 2 cut(s) 274, 719
NspV TTCGAA 2 cut(s) 112, 152
PaeI GCATGC 2 cut(s) 274, 719
PctI GAATGC 1 cut(s) 407
PfeI GAWTC 4 cut(s) 109, 328, 658, 751
PkrI GCNGC 4 cut(s) 35, 337, 367, 735
Ple19I CGATCG 1 cut(s) 289
PshBI ATTAAT 1 cut(s) 416
Psp6I CCWGG 2 cut(s) 763, 769
PspFI CCCAGC 1 cut(s) 678
PspGI CCWGG 2 cut(s) 763, 769
PspPI GGNCC 3 cut(s) 4, 767, 773
PstI CTGCAG 2 cut(s) 340, 735
PsuI RGATCY 1 cut(s) 451
PvuI CGATCG 1 cut(s) 289
PvuII CAGCTG 1 cut(s) 475
RsaI GTAC 2 cut(s) 392, 792
RsaNI GTAC 2 cut(s) 391, 791
RseI CAYNNNNRTG 1 cut(s) 269
SaqAI TTAA 4 cut(s) 72, 174, 416, 522
SatI GCNGC 4 cut(s) 34, 336, 366, 734
Sau3AI GATC 2 cut(s) 286, 451
Sau96I GGNCC 3 cut(s) 4, 767, 773
ScaI AGTACT 1 cut(s) 392
ScrFI CCNGG 2 cut(s) 765, 771
SfaNI GCATC 3 cut(s) 196, 322, 702
SfcI CTRYAG 3 cut(s) 336, 455, 731
SfuI TTCGAA 2 cut(s) 112, 152
SinI GGWCC 2 cut(s) 4, 773
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 2 cut(s) 173, 373
SmoI CTYRAG 2 cut(s) 173, 373
SphI GCATGC 2 cut(s) 274, 719
Sse9I AATT 7 cut(s) 102, 124, 383, 413, 460, 555, 824
SsiI CCGC 1 cut(s) 36
SspMI CTAG 3 cut(s) 234, 261, 267
StyD4I CCNGG 2 cut(s) 763, 769
TaaI ACNGT 2 cut(s) 53, 585
TaqI TCGA 6 cut(s) 112, 128, 152, 285, 319, 661
TasI AATT 7 cut(s) 102, 124, 383, 413, 460, 555, 824
TatI WGTACW 1 cut(s) 390
TfiI GAWTC 4 cut(s) 109, 328, 658, 751
Tru1I TTAA 4 cut(s) 72, 174, 416, 522
Tru9I TTAA 4 cut(s) 72, 174, 416, 522
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 4 cut(s) 33, 335, 365, 733
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 4 cut(s) 543, 550, 657, 801
Vha464I CTTAAG 1 cut(s) 173
VpaK11BI GGWCC 2 cut(s) 4, 773
VspI ATTAAT 1 cut(s) 416
XapI RAATTY 2 cut(s) 460, 555
XceI RCATGY 2 cut(s) 274, 719
XspI CTAG 3 cut(s) 234, 261, 267
ZrmI AGTACT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.