Rroxscaffold_7G00198220
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011645
Physical Location & Seq
Forward (+)
43299381 .. 43327789
28409 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00198220.1

Sequence Viewer

Length: 756 bp
ATGGGGAGAGGCAAGATTGTGATCCGAAGGATTGACAATTCGACGAGCAGACAAGTGACGTTTTCCAAGCGAAGAAATGGGCTGCTGAAGAAGGCTAAGGAGCTTTCAGTCCTTTGTGATGCTGAGGTTGGATTGATCATCTTCTCCAGCACCGGCAGGCTCTATGATTATGCTAGCACTAGCATGAAATCAGTTATTGATCGCTATAAAAGGCTCAAAGAGGAACATCACCAACTGTTGAATCCTGCTTCTGAAGTCAAGTTTTGGGAAAGGGAGGCAGCAAGCTTGAGACAACAATTGCAGTACTTGCAAGAATGCCACAGGCAATTGATGGGTGAAGAACTATCTGGTTTGAGCACCAAAGATCTACAGAACCTGGAAAGCAAACTGGAGATGAGTTTGAAAGGTGTCCGGTTGAAAAAGGACCAGCTATTAAATGAAGAAATAAAAGACCTGAGCAAGAAGGGAAATCTCATACATCAAGAGAACATAGAACTGTATAAGAAGCTAGACGTCTTAGGTAAAGAGAATGTGGAACTCCAAAGAAAGGCCTATGGAGCAAGGAATGTGAATGAAGAAAACGAAAGCTCCGAACCTTCGTTTACCATTACTAATGGATATCAATTGCATGCACCCATCCATCTGCAGCTAAGCCAGCCACAGAATCAAACTGAGCCTCAGCCTCAGAACAATGTACCAGCAAGTGCGATCAAATTAGGGTATCACTTAAATTCTCACTATTTTGATGAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

251

Amino Acids

28.92

Weight (kDa)

8.87

Isoelectric Point (pI)

54.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.3e-25 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 83 - 170 4.5e-26 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 516
AclWI GGATC 1 cut(s) 16
AcsI RAATTY 1 cut(s) 730
AcuI CTGAAG 2 cut(s) 107, 273
AcyI GRCGYC 1 cut(s) 513
AfaI GTAC 2 cut(s) 305, 696
AfiI CCNNNNNNNGG 1 cut(s) 547
AgsI TTSAA 3 cut(s) 241, 403, 418
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 6 cut(s) 103, 285, 430, 508, 588, 649
AluI AGCT 6 cut(s) 103, 285, 430, 508, 588, 649
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 1 cut(s) 283
AlwI GGATC 1 cut(s) 16
AlwNI CAGNNNCTG 1 cut(s) 376
AoxI GGCC 1 cut(s) 549
ApeKI GCWGC 3 cut(s) 82, 278, 646
ApoI RAATTY 1 cut(s) 730
AspS9I GGNCC 1 cut(s) 424
AsuHPI GGTGA 2 cut(s) 221, 347
AsuNHI GCTAGC 1 cut(s) 173
AvaII GGWCC 1 cut(s) 424
Bbv12I GWGCWC 1 cut(s) 359
BbvCI CCTCAGC 2 cut(s) 123, 678
BbvI GCAGC 3 cut(s) 69, 290, 658
BccI CCATC 3 cut(s) 325, 644, 648
BciT130I CCWGG 1 cut(s) 377
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 283
BfaI CTAG 4 cut(s) 174, 180, 509, 754
BfmI CTRYAG 2 cut(s) 368, 644
BglII AGATCT 1 cut(s) 364
BisI GCNGC 3 cut(s) 83, 279, 647
BlpI GCTNAGC 1 cut(s) 650
BlsI GCNGC 3 cut(s) 84, 280, 648
BmcAI AGTACT 1 cut(s) 305
Bme1390I CCNGG 1 cut(s) 377
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 424
BmrFI CCNGG 1 cut(s) 377
BmsI GCATC 1 cut(s) 109
BmtI GCTAGC 1 cut(s) 177
BpmI CTGGAG 2 cut(s) 130, 410
Bpu10I CCTNAGC 4 cut(s) 96, 123, 455, 678
Bpu1102I GCTNAGC 1 cut(s) 650
BpuEI CTTGAG 1 cut(s) 307
BsaBI GATNNNNATC 1 cut(s) 20
BsaHI GRCGYC 1 cut(s) 513
BsaWI WCCGGW 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 572, 602
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 1 cut(s) 393
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 1 cut(s) 377
BseGI GGATG 1 cut(s) 636
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 5 cut(s) 114, 446, 663, 692, 698
BseNI ACTGG 1 cut(s) 393
BseXI GCAGC 3 cut(s) 69, 290, 658
BshFI GGCC 1 cut(s) 551
BsiHKAI GWGCWC 1 cut(s) 359
BsiSI CCGG 2 cut(s) 153, 412
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 1 cut(s) 283
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 359
Bsp143I GATC 5 cut(s) 21, 135, 199, 364, 708
Bsp1720I GCTNAGC 1 cut(s) 650
BspANI GGCC 1 cut(s) 551
BspCNI CTCAG 5 cut(s) 115, 447, 664, 691, 697
BspMAI CTGCAG 1 cut(s) 648
BspOI GCTAGC 1 cut(s) 177
BspPI GGATC 1 cut(s) 16
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 1 cut(s) 393
BssAI RCCGGY 1 cut(s) 152
BssMI GATC 5 cut(s) 21, 135, 199, 364, 708
BssNI GRCGYC 1 cut(s) 513
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 2 cut(s) 237, 498
BstACI GRCGYC 1 cut(s) 513
BstAPI GCANNNNNTGC 1 cut(s) 307
BstC8I GCNNGC 5 cut(s) 158, 175, 283, 630, 656
BstDEI CTNAG 8 cut(s) 96, 123, 455, 517, 650, 672, 678, 684
BstF5I GGATG 1 cut(s) 636
BstKTI GATC 5 cut(s) 24, 138, 202, 367, 711
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 5 cut(s) 21, 135, 199, 364, 708
BstMWI GCNNNNNNNGC 3 cut(s) 307, 557, 655
BstNI CCWGG 1 cut(s) 377
BstNSI RCATGY 1 cut(s) 632
BstSCI CCNGG 1 cut(s) 375
BstSFI CTRYAG 2 cut(s) 368, 644
BstV1I GCAGC 3 cut(s) 69, 290, 658
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuRI GGCC 1 cut(s) 551
BtsCI GGATG 1 cut(s) 636
Cac8I GCNNGC 5 cut(s) 158, 175, 283, 630, 656
CaiI CAGNNNCTG 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 424
Csp6I GTAC 2 cut(s) 304, 695
CviAII CATG 2 cut(s) 184, 629
CviQI GTAC 2 cut(s) 304, 695
DdeI CTNAG 8 cut(s) 96, 123, 455, 517, 650, 672, 678, 684
DpnI GATC 5 cut(s) 23, 137, 201, 366, 710
DpnII GATC 5 cut(s) 21, 135, 199, 364, 708
Eco147I AGGCCT 1 cut(s) 551
Eco32I GATATC 1 cut(s) 620
Eco47I GGWCC 1 cut(s) 424
Eco57I CTGAAG 2 cut(s) 107, 273
EcoRII CCWGG 1 cut(s) 375
EcoRV GATATC 1 cut(s) 620
FaeI CATG 2 cut(s) 187, 632
FaiI YATR 9 cut(s) 165, 171, 185, 207, 476, 491, 501, 555, 630
FatI CATG 2 cut(s) 183, 628
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 3 cut(s) 83, 279, 647
FokI GGATG 1 cut(s) 623
Fsp4HI GCNGC 3 cut(s) 83, 279, 647
FspBI CTAG 4 cut(s) 174, 180, 509, 754
GluI GCNGC 3 cut(s) 83, 279, 647
GsuI CTGGAG 2 cut(s) 130, 410
HaeIII GGCC 1 cut(s) 551
HapII CCGG 2 cut(s) 153, 412
Hin1I GRCGYC 1 cut(s) 513
Hin1II CATG 2 cut(s) 187, 632
HindIII AAGCTT 1 cut(s) 283
HinfI GANTC 2 cut(s) 241, 664
HpaII CCGG 2 cut(s) 153, 412
HphI GGTGA 2 cut(s) 221, 347
Hpy166II GTNNAC 1 cut(s) 603
Hpy188I TCNGA 4 cut(s) 26, 253, 592, 687
Hpy188III TCNNGA 1 cut(s) 482
Hpy8I GTNNAC 1 cut(s) 603
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 4 cut(s) 21, 85, 457, 606
HpyCH4III ACNGT 2 cut(s) 237, 498
HpyCH4IV ACGT 2 cut(s) 59, 513
HpyCH4V TGCA 5 cut(s) 301, 310, 628, 632, 646
HpyF10VI GCNNNNNNNGC 3 cut(s) 307, 557, 655
HpyF3I CTNAG 8 cut(s) 96, 123, 455, 517, 650, 672, 678, 684
HpySE526I ACGT 2 cut(s) 59, 513
Hsp92I GRCGYC 1 cut(s) 513
Hsp92II CATG 2 cut(s) 187, 632
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 5 cut(s) 21, 135, 199, 364, 708
LmnI GCTCC 3 cut(s) 100, 557, 593
Lsp1109I GCAGC 3 cut(s) 69, 290, 658
LweI GCATC 1 cut(s) 109
MaeI CTAG 4 cut(s) 174, 180, 509, 754
MaeII ACGT 2 cut(s) 59, 513
MaeIII GTNAC 1 cut(s) 55
MalI GATC 5 cut(s) 23, 137, 201, 366, 710
MboI GATC 5 cut(s) 21, 135, 199, 364, 708
MboII GAAGA 6 cut(s) 84, 100, 133, 350, 452, 587
MfeI CAATTG 3 cut(s) 296, 326, 623
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 1 cut(s) 359
MluCI AATT 6 cut(s) 37, 296, 326, 623, 713, 730
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 5 cut(s) 118, 214, 268, 687, 693
MseI TTAA 2 cut(s) 434, 728
MslI CAYNNNNRTG 1 cut(s) 182
MspI CCGG 2 cut(s) 153, 412
MspR9I CCNGG 1 cut(s) 377
MunI CAATTG 3 cut(s) 296, 326, 623
Mva1269I GAATGC 1 cut(s) 320
MvaI CCWGG 1 cut(s) 377
MwoI GCNNNNNNNGC 3 cut(s) 307, 557, 655
NdeII GATC 5 cut(s) 21, 135, 199, 364, 708
NheI GCTAGC 1 cut(s) 173
NlaIII CATG 2 cut(s) 187, 632
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 1 cut(s) 632
PaeI GCATGC 1 cut(s) 632
PceI AGGCCT 1 cut(s) 551
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PctI GAATGC 1 cut(s) 320
PfeI GAWTC 2 cut(s) 241, 664
PkrI GCNGC 3 cut(s) 84, 280, 648
Psp6I CCWGG 1 cut(s) 375
PspGI CCWGG 1 cut(s) 375
PspPI GGNCC 1 cut(s) 424
PstI CTGCAG 1 cut(s) 648
PstNI CAGNNNCTG 1 cut(s) 376
PsuI RGATCY 1 cut(s) 364
RsaI GTAC 2 cut(s) 305, 696
RsaNI GTAC 2 cut(s) 304, 695
RseI CAYNNNNRTG 1 cut(s) 182
SaqAI TTAA 2 cut(s) 434, 728
SatI GCNGC 3 cut(s) 83, 279, 647
Sau3AI GATC 5 cut(s) 21, 135, 199, 364, 708
Sau96I GGNCC 1 cut(s) 424
ScaI AGTACT 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 1 cut(s) 359
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 2 cut(s) 368, 644
SinI GGWCC 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
SphI GCATGC 1 cut(s) 632
Sse9I AATT 6 cut(s) 37, 296, 326, 623, 713, 730
SseBI AGGCCT 1 cut(s) 551
SspMI CTAG 4 cut(s) 174, 180, 509, 754
StuI AGGCCT 1 cut(s) 551
StyD4I CCNGG 1 cut(s) 375
TaaI ACNGT 2 cut(s) 237, 498
TaiI ACGT 2 cut(s) 62, 516
TaqI TCGA 1 cut(s) 41
TasI AATT 6 cut(s) 37, 296, 326, 623, 713, 730
TatI WGTACW 1 cut(s) 303
TfiI GAWTC 2 cut(s) 241, 664
Tru1I TTAA 2 cut(s) 434, 728
Tru9I TTAA 2 cut(s) 434, 728
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 3 cut(s) 82, 278, 646
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 3 cut(s) 200, 453, 588
VpaK11BI GGWCC 1 cut(s) 424
XapI RAATTY 1 cut(s) 730
XceI RCATGY 1 cut(s) 632
XspI CTAG 4 cut(s) 174, 180, 509, 754
ZraI GACGTC 1 cut(s) 514
ZrmI AGTACT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.