pycom10g04380

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010240
Physical Location & Seq
Forward (+)
4604864 .. 4612482
7619 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3846 bp
ATGGGCAGTAAAAAGAGAAGCTCCACTTCCATGGAAGCTGCGGCTGATGCCGTCTTTGACGACGGCGGCTTCAGTAATCTGAAGAAATCGAAGAAGGGCAAAATAAAGCAGGAGACAGCAGAAGCTTCTGCCCCTTCTTCTTCTACCGCTACCACCGCCGTCAAACCCATGGAGAGACAAAAGAAAAGAAAAGCTTTGGACAAAGTGAGGCGCCTCCACACCGAGGAGACCAAACCCAAAGAACCTAAAACAATGGAGGTTCAATTGACAACTGAGGAGCCAGCTTCGTCTTCGACGAGCGGGATTCTTCCGGAGTTCCATGTGGGTGTGTTTAAGGACTTGGCTTCGGGTGATGGGTCTGTTAGAGAAGCTGCGGCGGAGGCTTTGGCTATGGAGTTGGTGGCGGTGCAGAGAGCTTATGATGGGCTTGAGAATAAGGAGTTGGTTGAAGGTGGAGTGAAGATGGAAGCCGAGAAGGATGATGGGTTGAATGATTGTGCGCCTTCCTTGAGATATGCCGTGCGGAGACTCATTCGCGGTGTTTCTTCATCAAGAGAGTGTGCAAGACAAGGGTTTGCATTAGGTTTAACTATTTTAGTAAGTACGATCCCCAGCATCAAGGTGAAGTCATTGTTGAAACTCATTGTTGATTTCTTAGAGGTTTCTTCATCAATGAAGGGTCAGGAACAAAGGGATCATCTTTTGGGTCGCTTATTTGCCTATGGAGCTATTGCCCGATCAGGAAGACTAGCTGAGGAGTGGGTTTCTGATAGAAATACTCCTCTCATAAAGGAATTCACTAGTCTTCTCATTGCCCTTGCATCAAAAAAGCGATACTTGCAGGAGCCTGTTGTTTTGGTTATTGTAGACTTGATTGAGAAGTTGCACTCGGAGGCCTTGCTGAACCACATGCTGGAGGCCCCAGGACTTAATGAGTGGTTTGAAGGAGCTATTCAAGTTGGAAATCCTGATGCATTGCTTCTGGCTCTGAAAATCAGAGAAAAAACACTTATTGAAAGTTCAAGTTTTGGGAACCTTTTACCAGTCCCATTCAGCCCCAACAAACTGTTTTCTGCTGACCATCTGTCTTCTCTTGCTAACTGCTTGAAGGAATCCACCTTTTGTCAGCCTCGTGTTCACAATGTGTGGCCTGTTCTGGTGAACATGCTTTTACCTGATAGAGTTTTGCAGGCTGAAGATGCAATTTCTGTTTCAAATTCCTTGAAGAGGCCTAAAAAGAATCGCAAGTCTAGCTCTTCTGATGAAGAAATTGCAAAGAATTTCCAGTGCTTCTGCGAGGTGATCATTGATCGATCACTTCTACCATCATCTCATGACCGCAAACACTTGGCCTTCGATGTTCTGCTTCTTCTTCTACCAAAGTTGCCGGCATCTTTTATTCCGATTAGTCTGTCTTACAAAATTGTTCAGTGCATGATTGATATACTTTCTACAAAGGATTCTTGGCTCTATAAAGTTCTGCAACATTTTCTTAAAACTTTAACTGATTGGGTTGGGGATGATGACGTGAGAAGGGTTTCTGTCATTGTGGCTTTGCAGAAACATAGCAATGGAAAATTTGATTGCATTACACGAACAAAGACAGTTAAGGATTTGATGTCAGATTTCAGAACAGAATCTGGTTGTATGCTGTTCATTCAGAACCTGTTGAACATGTTTCTGGATGAAAAACATGCTACCGAGGAACCTTCAGATCAAAGTCAAACGACAGATGACAATTCAGAGATAGGTTCTGTTGAAGACAAGGATTCCGTTGGAACCATGGGAAATTCTGATTTTTTGAAAACATGGATTGTGGAATCCCTCCCTAGTATATTGAAAAATTTGAAACTGGATCCCGAAGCAAAGTTTAGGGTCCAAAAAGGAATTTTGAAGTTTCTAGCAGTTCAGGGATTGTTCACCGCATCCCTTGGGTCTGAAGTAACATCCCTTGAATTACAAGAGACATTTAGGTGGCCAAAAGCTGCCACCTCAAGTGCCCTTTGCAGGATATGTATCGAGCAGCTTCAGTTACTGTTCGCAAACTCTCAGAAAGGAGAGGGGCCACGCCCTTTGCCAAATTGCATAGAGCAAATTGACCTTGGCTCATACTTTATGCGCTTTCTTAGCACTTTGTGCAGTATTCCTTCCGTCTCTTATTTTCGGCCTTTGGAAACCGGGGAAGAAAACACTTTGAAAAAATTGCAAGCAATGGAAACTTCACTCGCAAAAGAGGAAAGGAATTGTGGGCTTAGTGGTGATGCAAATAGGTTGCATGCACTAAGATACTTGCTCATCCAGCTGCTTTTGCAAATGCTACTTCGACCACAGGAATACTTAGAAGCAGTCTCCGAGCTTATTATATGTTGTAAGAAAGCTTTTCCTGTTGCTGATCTTCTTGATTCCCCTGGAGAAGATGACTTGGATGATGATGGTGCACCTGCCGTGATGGATGTTCTTGTGGATACATTGCTTTCTTTGCTGCCACAGTCATCAGCTCCCATGCGAACTGCAATTGAGCAGGTATTTAAATACTTCTGTGGTGATATCACGGATGATGGTCTGTTGCGGATGTTGAGGGTCATTAGGAAGAATTTGAAACCTGCTAGACATCAAGATGCTGATAGTGACGACATTTTTGACGATGAAGAAGATGATGAAGATTTTCTTAACATTGAAGAAGATGAGGGAATTGATAAAGCTGAGACAGGTGAAACCGGTGACAGTGATGAGCAGCCGGATGACTCCGAGGCTGAGTCTGAGGCTGTTGATGCTTTTGAGGCAGTTGGCAAAGAAAGTCCTGAAGCTTCTGATGATTCCGATGGAGGAATGGATGATGATGCAATGTTTCGAATGGATACTTACCTTACCCAGATCTTCAAAGAGCGAAAGAATCTAGCTGGGGGTGACACTGCCCATCACCAACTCATGCTGTTTAAGCTTCGTGTTCTTTCATTGCTAGAAATTTACCTACATGAAAATCCAGGCAAGCCGCAGGTTTTGTTGGTGTACTCAAACTTAGCTCGGGCATTTATTGAACCACCCAGTGCAGAAAGTAGCGAACAGCTAGGACAGCGTGTATGGGGGATACTGCAAAAGAAAATATTTAAAGCAAAGGACTATCCGAAGGGGGAAGATGTGCAATTATCTACTTTAGAATCTTTGCTGCAAAAGAATTTGAAGTTGGCCTCAAAACCAATTAAGAGAAAGAAATCCGCAACCAACTTGTCCAAGAAGAAACAGTCAGCCTCGTGGAACCGACACAAAATGATGACTAGCCTTGCACAGAGCTCCACTTTCTGGATTCTGAAGATCGTTCGGGAAGCAAGAAATTTTCCAGAGTCTGAGCTGCAGAGGGTTTTTGATATTTTCCAGGGAGTTCTAGTAGAGTATTTCAATAGTAAAAAGTCTCAAATCAAATCTGGATTTTTGAAAGAAATATTTAGAAGAAGGCCATGGGTAGGGCACCATCTCTTTGGCTTCCTTCTGGAGAAATGTGGCACTTCAAAGTCAGATTTTCGGCGAGTAGAAGCACTTGATTTGGTGAGTGAAATATTGAAGTCCCTGGGCTCTACTGATGGAAGTAGCCAGGAAGCGCTGAAGAATATTATGAAAAGCCATCTGGAGAAACTCTGTCGTCTGATAGAACAATTGCTGACAAATATATCAGAAAAACAGTCGAGGCAGGCTGAAGCGCGGAAGTTTTGTAGCAGGATTCTTCAGATGATAACAACCCTTAAGCTGACCAAGTCTTTCTTTAAAAATTTATCCCCAGATGCTCATGCCAAGTGTGAATCGCAACTCGGTGCCCAATTTATTAAAATGAAGACAGTAGCACATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

1282

Amino Acids

143.34

Weight (kDa)

5.95

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_phi PF04931 172 - 991 1.8e-177 DNA polymerase phi
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64420
fragaria_vesca FvH4_2g11160
malus_domestica MD05G1049800.v1.1 MD10G1056900.v1.1
prunus_persica Prupe.8G075100_v2.0.a1
pyrus_communis pycom05g04160 pycom05g04170 pycom10g04380
rosa_chinensis RchiOBHm_Chr6g0269481
rosa_laevigata RLG00000013848 RLG00000013849
rosa_multiflora Rmu_ssc0000291.1_g000033
rosa_roxburghii Rroxscaffold_7G00198700
rosa_rugosa Rorug06G0048500
rosa_samantha Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100
rosa_wichuraiana Rw6G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2449
Acc36I ACCTGC 4 cut(s) 2449, 2512, 2611, 2986
AccB1I GGYRCC 3 cut(s) 210, 3465, 3806
AccB7I CCANNNNNTGG 2 cut(s) 913, 3300
AccBSI CCGCTC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 867
AccII CGCG 2 cut(s) 537, 3697
AccIII TCCGGA 1 cut(s) 310
AclWI GGATC 4 cut(s) 601, 702, 1850, 1863
AcoI YGGCCR 1 cut(s) 1976
AcyI GRCGYC 1 cut(s) 211
AdeI CACNNNGTG 3 cut(s) 1144, 2136, 3047
AfaI GTAC 2 cut(s) 604, 3011
AfeI AGCGCT 1 cut(s) 3597
AfiI CCNNNNNNNGG 6 cut(s) 223, 913, 1227, 2007, 3300, 3461
AflII CTTAAG 1 cut(s) 3737
AflIII ACRYGT 1 cut(s) 1674
AgeI ACCGGT 1 cut(s) 2717
AhdI GACNNNNNGTC 1 cut(s) 1084
AhlI ACTAGT 1 cut(s) 800
AjiI CACGTC 1 cut(s) 1528
AjnI CCWGG 6 cut(s) 922, 2407, 2983, 3372, 3564, 3588
AjuI GAANNNNNNNTTGG 4 cut(s) 425, 457, 3167, 3199
AleI CACNNNNGTG 1 cut(s) 3841
Alw21I GWGCWC 2 cut(s) 2440, 3293
Alw26I GTCTC 9 cut(s) 107, 169, 221, 520, 1958, 2158, 2353, 2699, 3414
Alw44I GTGCAC 1 cut(s) 2436
AlwI GGATC 4 cut(s) 601, 702, 1850, 1863
AlwNI CAGNNNCTG 4 cut(s) 1640, 1666, 2035, 3344
Ama87I CYCGRG 1 cut(s) 3024
Aor13HI TCCGGA 1 cut(s) 310
Aor51HI AGCGCT 1 cut(s) 3597
ApaLI GTGCAC 1 cut(s) 2436
ApeKI GCWGC 9 cut(s) 38, 371, 1985, 2023, 2302, 2482, 2734, 3166, 3349
ArsI GACNNNNNNTTYG 2 cut(s) 2621, 2653
AsiGI ACCGGT 1 cut(s) 2717
Asp700I GAANNNNTTC 2 cut(s) 1537, 2664
AspLEI GCGC 5 cut(s) 213, 502, 2121, 3598, 3697
AspS9I GGNCC 3 cut(s) 919, 1876, 2063
AsuC2I CCSGG 1 cut(s) 2179
AsuII TTCGAA 1 cut(s) 2851
AvaI CYCGRG 1 cut(s) 3024
AvaII GGWCC 1 cut(s) 1876
BaeGI GKGCMC 4 cut(s) 2002, 2440, 3468, 3811
BalI TGGCCA 1 cut(s) 1978
BamHI GGATCC 1 cut(s) 1855
BanI GGYRCC 3 cut(s) 210, 3465, 3806
BanII GRGCYC 2 cut(s) 3293, 3572
BauI CACGAG 2 cut(s) 1131, 3250
BbsI GAAGAC 6 cut(s) 282, 751, 797, 1080, 1767, 3833
Bbv12I GWGCWC 2 cut(s) 2440, 3293
BbvCI CCTCAGC 1 cut(s) 753
BbvI GCAGC 9 cut(s) 25, 358, 1972, 2035, 2289, 2469, 2746, 3153, 3336
BceAI ACGGC 5 cut(s) 35, 79, 143, 503, 2429
BciT130I CCWGG 6 cut(s) 924, 2409, 2985, 3374, 3566, 3590
BciVI GTATCC 3 cut(s) 2458, 2851, 3081
BclI TGATCA 1 cut(s) 1302
BcnI CCSGG 1 cut(s) 2179
BcoDI GTCTC 9 cut(s) 107, 169, 221, 520, 1958, 2158, 2353, 2699, 3414
BcuI ACTAGT 1 cut(s) 800
BfmI CTRYAG 1 cut(s) 3350
BfoI RGCGCY 2 cut(s) 214, 3599
BfrI CTTAAG 1 cut(s) 3737
BfuAI ACCTGC 4 cut(s) 2449, 2512, 2611, 2986
BfuI GTATCC 3 cut(s) 2458, 2851, 3081
BglII AGATCT 1 cut(s) 2874
Bme1390I CCNGG 7 cut(s) 924, 2179, 2409, 2985, 3374, 3566, 3590
Bme18I GGWCC 1 cut(s) 1876
BmeRI GACNNNNNGTC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 3024
BmgBI CACGTC 1 cut(s) 1528
BmgT120I GGNCC 3 cut(s) 919, 1876, 2063
BmrFI CCNGG 7 cut(s) 924, 2179, 2409, 2985, 3374, 3566, 3590
BmrI ACTGGG 1 cut(s) 3039
BmuI ACTGGG 1 cut(s) 3039
BpiI GAAGAC 6 cut(s) 282, 751, 797, 1080, 1767, 3833
BpmI CTGGAG 4 cut(s) 935, 2430, 3509, 3644
Bpu10I CCTNAGC 1 cut(s) 753
Bpu14I TTCGAA 1 cut(s) 2851
BpuEI CTTGAG 3 cut(s) 449, 529, 1978
BpuMI CCSGG 1 cut(s) 2179
Bsa29I ATCGAT 1 cut(s) 1312
BsaBI GATNNNNATC 1 cut(s) 2015
BsaHI GRCGYC 1 cut(s) 211
BsaI GGTCTC 1 cut(s) 221
BsaWI WCCGGW 2 cut(s) 310, 2717
BsaXI ACNNNNNCTCC 2 cut(s) 3369, 3399
Bsc4I CCNNNNNNNGG 6 cut(s) 223, 913, 1227, 2007, 3300, 3461
Bse118I RCCGGY 2 cut(s) 1387, 2717
Bse1I ACTGG 4 cut(s) 1043, 1285, 1857, 3045
Bse3DI GCAATG 7 cut(s) 810, 974, 1576, 2217, 2468, 2850, 2954
Bse8I GATNNNNATC 1 cut(s) 2015
BseAI TCCGGA 1 cut(s) 310
BseBI CCWGG 6 cut(s) 924, 2409, 2985, 3374, 3566, 3590
BseCI ATCGAT 1 cut(s) 1312
BseJI GATNNNNATC 1 cut(s) 2015
BseLI CCNNNNNNNGG 6 cut(s) 223, 913, 1227, 2007, 3300, 3461
BseMI GCAATG 7 cut(s) 810, 974, 1576, 2217, 2468, 2850, 2954
BseMII CTCAG 7 cut(s) 264, 744, 2063, 2694, 2745, 2751, 3336
BseNI ACTGG 4 cut(s) 1043, 1285, 1857, 3045
BseRI GAGGAG 4 cut(s) 239, 290, 770, 771
BseSI GKGCMC 4 cut(s) 2002, 2440, 3468, 3811
BseXI GCAGC 9 cut(s) 25, 358, 1972, 2035, 2289, 2469, 2746, 3153, 3336
BseYI CCCAGC 2 cut(s) 611, 2900
BsgI GTGCAG 3 cut(s) 428, 2158, 3069
Bsh1236I CGCG 2 cut(s) 537, 3697
BshNI GGYRCC 3 cut(s) 210, 3465, 3806
BshTI ACCGGT 1 cut(s) 2717
BshVI ATCGAT 1 cut(s) 1312
BsiHKAI GWGCWC 2 cut(s) 2440, 3293
BsiHKCI CYCGRG 1 cut(s) 3024
BsiSI CCGG 5 cut(s) 311, 1388, 2178, 2718, 2738
BslFI GGGAC 2 cut(s) 1031, 3547
BslI CCNNNNNNNGG 6 cut(s) 223, 913, 1227, 2007, 3300, 3461
BsmAI GTCTC 9 cut(s) 107, 169, 221, 520, 1958, 2158, 2353, 2699, 3414
BsmBI CGTCTC 1 cut(s) 2158
BsmFI GGGAC 2 cut(s) 1031, 3547
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 3024
Bsp119I TTCGAA 1 cut(s) 2851
Bsp1286I GDGCHC 6 cut(s) 2002, 2440, 3293, 3468, 3572, 3811
Bsp13I TCCGGA 1 cut(s) 310
Bsp19I CCATGG 4 cut(s) 30, 168, 1782, 3455
BspCNI CTCAG 7 cut(s) 265, 745, 2062, 2695, 2746, 2752, 3337
BspDI ATCGAT 1 cut(s) 1312
BspEI TCCGGA 1 cut(s) 310
BspFNI CGCG 2 cut(s) 537, 3697
BspHI TCATGA 1 cut(s) 1333
BspMAI CTGCAG 1 cut(s) 3354
BspMI ACCTGC 4 cut(s) 2449, 2512, 2611, 2986
BspPI GGATC 4 cut(s) 601, 702, 1850, 1863
BspQI GCTCTTC 1 cut(s) 1261
BspT104I TTCGAA 1 cut(s) 2851
BspT107I GGYRCC 3 cut(s) 210, 3465, 3806
BspTI CTTAAG 1 cut(s) 3737
BspTNI GGTCTC 1 cut(s) 221
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 7 cut(s) 810, 974, 1576, 2217, 2468, 2850, 2954
BsrFI RCCGGY 2 cut(s) 1387, 2717
BsrI ACTGG 4 cut(s) 1043, 1285, 1857, 3045
BssAI RCCGGY 2 cut(s) 1387, 2717
BssNI GRCGYC 1 cut(s) 211
BssSI CACGAG 2 cut(s) 1131, 3250
BssT1I CCWWGG 6 cut(s) 30, 168, 1782, 1930, 2101, 3455
Bst2BI CACGAG 2 cut(s) 1131, 3250
Bst2UI CCWGG 6 cut(s) 924, 2409, 2985, 3374, 3566, 3590
Bst4CI ACNGT 8 cut(s) 1068, 1606, 2037, 2490, 2726, 3243, 3678, 3832
Bst6I CTCTTC 2 cut(s) 1220, 1261
BstACI GRCGYC 1 cut(s) 211
BstAFI CTTAAG 1 cut(s) 3737
BstAPI GCANNNNNTGC 1 cut(s) 2136
BstBI TTCGAA 1 cut(s) 2851
BstC8I GCNNGC 7 cut(s) 282, 1191, 1389, 2208, 2277, 2990, 3687
BstDSI CCRYGG 4 cut(s) 30, 168, 1782, 3455
BstENI CCTNNNNNAGG 1 cut(s) 1225
BstFNI CGCG 2 cut(s) 537, 3697
BstH2I RGCGCY 2 cut(s) 214, 3599
BstHHI GCGC 5 cut(s) 213, 502, 2121, 3598, 3697
BstMAI GTCTC 9 cut(s) 107, 169, 221, 520, 1958, 2158, 2353, 2699, 3414
BstNI CCWGG 6 cut(s) 924, 2409, 2985, 3374, 3566, 3590
BstNSI RCATGY 5 cut(s) 913, 1168, 1678, 1697, 2279
BstSCI CCNGG 7 cut(s) 922, 2177, 2407, 2983, 3372, 3564, 3588
BstSFI CTRYAG 1 cut(s) 3350
BstSLI GKGCMC 4 cut(s) 2002, 2440, 3468, 3811
BstUI CGCG 2 cut(s) 537, 3697
BstV1I GCAGC 9 cut(s) 25, 358, 1972, 2035, 2289, 2469, 2746, 3153, 3336
BstV2I GAAGAC 6 cut(s) 282, 751, 797, 1080, 1767, 3833
BstX2I RGATCY 2 cut(s) 1855, 2874
BstXI CCANNNNNNTGG 2 cut(s) 31, 3476
BstYI RGATCY 2 cut(s) 1855, 2874
Bsu15I ATCGAT 1 cut(s) 1312
BsuI GTATCC 3 cut(s) 2458, 2851, 3081
BsuTUI ATCGAT 1 cut(s) 1312
BtgI CCRYGG 4 cut(s) 30, 168, 1782, 3455
BtrI CACGTC 1 cut(s) 1528
BtsI GCAGTG 1 cut(s) 2910
BtsIMutI CAGTG 5 cut(s) 1292, 1436, 2731, 2910, 3052
BveI ACCTGC 4 cut(s) 2449, 2512, 2611, 2986
Cac8I GCNNGC 7 cut(s) 282, 1191, 1389, 2208, 2277, 2990, 3687
CaiI CAGNNNCTG 4 cut(s) 1640, 1666, 2035, 3344
CciI TCATGA 1 cut(s) 1333
CfoI GCGC 5 cut(s) 213, 502, 2121, 3598, 3697
Cfr10I RCCGGY 2 cut(s) 1387, 2717
Cfr13I GGNCC 3 cut(s) 919, 1876, 2063
ClaI ATCGAT 1 cut(s) 1312
Csp6I GTAC 2 cut(s) 603, 3010
CspAI ACCGGT 1 cut(s) 2717
CspCI CAANNNNNGTGG 2 cut(s) 2055, 2090
CviQI GTAC 2 cut(s) 603, 3010
DinI GGCGCC 1 cut(s) 212
DraI TTTAAA 3 cut(s) 2530, 3109, 3760
DraIII CACNNNGTG 3 cut(s) 1144, 2136, 3047
DriI GACNNNNNGTC 1 cut(s) 1084
EaeI YGGCCR 1 cut(s) 1976
Eam1104I CTCTTC 2 cut(s) 1220, 1261
Eam1105I GACNNNNNGTC 1 cut(s) 1084
EarI CTCTTC 2 cut(s) 1220, 1261
EciI GGCGGA 1 cut(s) 392
Ecl136II GAGCTC 1 cut(s) 3291
Eco130I CCWWGG 6 cut(s) 30, 168, 1782, 1930, 2101, 3455
Eco147I AGGCCT 2 cut(s) 896, 1231
Eco24I GRGCYC 2 cut(s) 3293, 3572
Eco31I GGTCTC 1 cut(s) 221
Eco32I GATATC 1 cut(s) 2548
Eco47I GGWCC 1 cut(s) 1876
Eco47III AGCGCT 1 cut(s) 3597
Eco53kI GAGCTC 1 cut(s) 3291
Eco88I CYCGRG 1 cut(s) 3024
EcoICRI GAGCTC 1 cut(s) 3291
EcoNI CCTNNNNNAGG 1 cut(s) 1225
EcoO109I RGGNCCY 1 cut(s) 919
EcoRI GAATTC 1 cut(s) 794
EcoRII CCWGG 6 cut(s) 922, 2407, 2983, 3372, 3564, 3588
EcoRV GATATC 1 cut(s) 2548
EcoT14I CCWWGG 6 cut(s) 30, 168, 1782, 1930, 2101, 3455
EcoT22I ATGCAT 1 cut(s) 976
EcoT38I GRGCYC 2 cut(s) 3293, 3572
EgeI GGCGCC 1 cut(s) 212
EheI GGCGCC 1 cut(s) 212
ErhI CCWWGG 6 cut(s) 30, 168, 1782, 1930, 2101, 3455
Esp3I CGTCTC 1 cut(s) 2158
FaqI GGGAC 2 cut(s) 1031, 3547
FauI CCCGC 1 cut(s) 293
FbaI TGATCA 1 cut(s) 1302
FblI GTMKAC 1 cut(s) 867
FriOI GRGCYC 2 cut(s) 3293, 3572
GlaI GCGC 5 cut(s) 212, 501, 2120, 3597, 3696
GsaI CCCAGC 2 cut(s) 615, 2904
GsuI CTGGAG 4 cut(s) 935, 2430, 3509, 3644
HaeII RGCGCY 2 cut(s) 214, 3599
HapII CCGG 5 cut(s) 311, 1388, 2178, 2718, 2738
HhaI GCGC 5 cut(s) 213, 502, 2121, 3598, 3697
Hin1I GRCGYC 1 cut(s) 211
Hin6I GCGC 5 cut(s) 211, 500, 2119, 3596, 3695
HinP1I GCGC 5 cut(s) 211, 500, 2119, 3596, 3695
HindIII AAGCTT 5 cut(s) 123, 192, 2376, 2805, 2939
HpaII CCGG 5 cut(s) 311, 1388, 2178, 2718, 2738
Hpy166II GTNNAC 6 cut(s) 868, 1138, 1162, 1920, 2438, 3010
Hpy8I GTNNAC 6 cut(s) 868, 1138, 1162, 1920, 2438, 3010
Hpy99I CGWCG 2 cut(s) 65, 298
HpyCH4III ACNGT 8 cut(s) 1068, 1606, 2037, 2490, 2726, 3243, 3678, 3832
HpyCH4IV ACGT 1 cut(s) 1527
HpySE526I ACGT 1 cut(s) 1527
Hsp92I GRCGYC 1 cut(s) 211
HspAI GCGC 5 cut(s) 211, 500, 2119, 3596, 3695
KasI GGCGCC 1 cut(s) 210
Kpn2I TCCGGA 1 cut(s) 310
KroI GCCGGC 1 cut(s) 1387
KroNI GCCGGC 1 cut(s) 1389
Ksp22I TGATCA 1 cut(s) 1302
LguI GCTCTTC 1 cut(s) 1261
LmnI GCTCC 7 cut(s) 26, 277, 725, 844, 947, 2503, 3296
Lsp1109I GCAGC 9 cut(s) 25, 358, 1972, 2035, 2289, 2469, 2746, 3153, 3336
MaeII ACGT 1 cut(s) 1527
MaeIII GTNAC 5 cut(s) 1942, 2031, 2627, 2720, 2906
MbiI CCGCTC 1 cut(s) 300
MfeI CAATTG 3 cut(s) 263, 2514, 3650
MflI RGATCY 2 cut(s) 1855, 2874
MhlI GDGCHC 6 cut(s) 2002, 2440, 3293, 3468, 3572, 3811
MlsI TGGCCA 1 cut(s) 1978
MluNI TGGCCA 1 cut(s) 1978
Mly113I GGCGCC 1 cut(s) 211
MlyI GAGTC 4 cut(s) 522, 2738, 2765, 3350
MmeI TCCRAC 2 cut(s) 940, 1756
Mox20I TGGCCA 1 cut(s) 1978
Mph1103I ATGCAT 1 cut(s) 976
MroI TCCGGA 1 cut(s) 310
MroNI GCCGGC 1 cut(s) 1387
MroXI GAANNNNTTC 2 cut(s) 1537, 2664
MscI TGGCCA 1 cut(s) 1978
MslI CAYNNNNRTG 7 cut(s) 29, 324, 620, 1569, 1972, 2616, 3841
Msp20I TGGCCA 1 cut(s) 1978
MspA1I CMGCKG 1 cut(s) 2302
MspCI CTTAAG 1 cut(s) 3737
MspI CCGG 5 cut(s) 311, 1388, 2178, 2718, 2738
MspR9I CCNGG 7 cut(s) 924, 2179, 2409, 2985, 3374, 3566, 3590
MunI CAATTG 3 cut(s) 263, 2514, 3650
MvaI CCWGG 6 cut(s) 924, 2409, 2985, 3374, 3566, 3590
MvnI CGCG 2 cut(s) 537, 3697
NaeI GCCGGC 1 cut(s) 1389
NarI GGCGCC 1 cut(s) 211
NciI CCSGG 1 cut(s) 2179
NcoI CCATGG 4 cut(s) 30, 168, 1782, 3455
NgoMIV GCCGGC 1 cut(s) 1387
NmeAIII GCCGAG 1 cut(s) 496
NmuCI GTSAC 3 cut(s) 2627, 2720, 2906
NsiI ATGCAT 1 cut(s) 976
NspI RCATGY 5 cut(s) 913, 1168, 1678, 1697, 2279
NspV TTCGAA 1 cut(s) 2851
OliI CACNNNNGTG 1 cut(s) 3841
PaeI GCATGC 1 cut(s) 2279
PagI TCATGA 1 cut(s) 1333
PaqCI CACCTGC 1 cut(s) 2449
PasI CCCWGGG 1 cut(s) 3565
PceI AGGCCT 2 cut(s) 896, 1231
PciI ACATGT 1 cut(s) 1674
PciSI GCTCTTC 1 cut(s) 1261
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PdiI GCCGGC 1 cut(s) 1389
PdmI GAANNNNTTC 2 cut(s) 1537, 2664
PflFI GACNNNGTC 1 cut(s) 3748
PflMI CCANNNNNTGG 2 cut(s) 913, 3300
PinAI ACCGGT 1 cut(s) 2717
PleI GAGTC 4 cut(s) 522, 2738, 2764, 3349
PluTI GGCGCC 1 cut(s) 214
PpsI GAGTC 4 cut(s) 522, 2738, 2764, 3349
PscI ACATGT 1 cut(s) 1674
Psp124BI GAGCTC 1 cut(s) 3293
Psp6I CCWGG 6 cut(s) 922, 2407, 2983, 3372, 3564, 3588
PspFI CCCAGC 2 cut(s) 611, 2900
PspGI CCWGG 6 cut(s) 922, 2407, 2983, 3372, 3564, 3588
PspPI GGNCC 3 cut(s) 919, 1876, 2063
PstI CTGCAG 1 cut(s) 3354
PstNI CAGNNNCTG 4 cut(s) 1640, 1666, 2035, 3344
PsuI RGATCY 2 cut(s) 1855, 2874
PsyI GACNNNGTC 1 cut(s) 3748
PvuII CAGCTG 1 cut(s) 2302
RsaI GTAC 2 cut(s) 604, 3011
RsaNI GTAC 2 cut(s) 603, 3010
RseI CAYNNNNRTG 7 cut(s) 29, 324, 620, 1569, 1972, 2616, 3841
SacI GAGCTC 1 cut(s) 3293
SapI GCTCTTC 1 cut(s) 1261
Sau96I GGNCC 3 cut(s) 919, 1876, 2063
SchI GAGTC 4 cut(s) 522, 2738, 2765, 3350
ScrFI CCNGG 7 cut(s) 924, 2179, 2409, 2985, 3374, 3566, 3590
SduI GDGCHC 6 cut(s) 2002, 2440, 3293, 3468, 3572, 3811
SfcI CTRYAG 1 cut(s) 3350
SfoI GGCGCC 1 cut(s) 212
SfuI TTCGAA 1 cut(s) 2851
SinI GGWCC 1 cut(s) 1876
SmiI ATTTAAAT 1 cut(s) 2530
SmiMI CAYNNNNRTG 7 cut(s) 29, 324, 620, 1569, 1972, 2616, 3841
SmlI CTYRAG 4 cut(s) 428, 508, 1993, 3737
SmoI CTYRAG 4 cut(s) 428, 508, 1993, 3737
SpeI ACTAGT 1 cut(s) 800
SphI GCATGC 1 cut(s) 2279
SseBI AGGCCT 2 cut(s) 896, 1231
SspDI GGCGCC 1 cut(s) 210
SspI AATATT 4 cut(s) 3105, 3441, 3555, 3607
SstI GAGCTC 1 cut(s) 3293
StuI AGGCCT 2 cut(s) 896, 1231
StyD4I CCNGG 7 cut(s) 922, 2177, 2407, 2983, 3372, 3564, 3588
StyI CCWWGG 6 cut(s) 30, 168, 1782, 1930, 2101, 3455
SwaI ATTTAAAT 1 cut(s) 2530
TaaI ACNGT 8 cut(s) 1068, 1606, 2037, 2490, 2726, 3243, 3678, 3832
TaiI ACGT 1 cut(s) 1530
TaqI TCGA 8 cut(s) 89, 293, 1312, 1356, 2019, 2323, 2851, 3680
TatI WGTACW 1 cut(s) 3009
TauI GCSGC 4 cut(s) 44, 69, 377, 2995
TscAI CASTG 5 cut(s) 1292, 1436, 2731, 2917, 3052
TseFI GTSAC 3 cut(s) 2627, 2720, 2906
TseI GCWGC 9 cut(s) 38, 371, 1985, 2023, 2302, 2482, 2734, 3166, 3349
Tsp45I GTSAC 3 cut(s) 2627, 2720, 2906
TspGWI ACGGA 3 cut(s) 1762, 2140, 2567
TspRI CASTG 5 cut(s) 1292, 1436, 2731, 2917, 3052
Tth111I GACNNNGTC 1 cut(s) 3748
Van91I CCANNNNNTGG 2 cut(s) 913, 3300
Vha464I CTTAAG 1 cut(s) 3737
VneI GTGCAC 1 cut(s) 2436
VpaK11BI GGWCC 1 cut(s) 1876
XagI CCTNNNNNAGG 1 cut(s) 1225
XceI RCATGY 5 cut(s) 913, 1168, 1678, 1697, 2279
XmiI GTMKAC 1 cut(s) 867
XmnI GAANNNNTTC 2 cut(s) 1537, 2664
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.