pycom10g05530

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003852
Physical Location & Seq
Reverse (-)
5928537 .. 5931366
2830 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g05530.3

Sequence Viewer

Length: 906 bp
ATGATCGATCCAAAGGCTGCCGAAAATTTCCAGGCACTGGGCGAGGCTTATCAGGTTTTAAGTGATCCAGAGAAGCGTGAAGCATATGACAAGCACGGAAAGGCAGGCATACCACAGGATTCCATGGTAGACCCTGCAGCTGTGTTTGGGATGCTTTTTGGGAGCGAATTCTTTGAAGATTATGTTGGTCAACTTGCTTTAGCTTCTTTGTCATCTGTTGAAATTGAAGAAGAGACACAGGACCCTGAAGTCCGTAGGCAGAAAGTCACGGAGAAAATGAGGACCTTGCAGAAGGAAAGGGAAGTAAAGCTCATAACTATTCTGAAAGATCGCCTTCAACCATTTGTTGATGGTCGTAATGATGAGTTTGTAAAATGGGCAAATTCAGAAGCACAACGCCTCTCATCAGCAGCCTTTGGTGAGGCTATGCTGCATACGATTGGTTATATTTACACGAGGCAAGCTGCAAGAGAAATTGGAAAAGACAAACGATACATGAAGGTTCCATTTTTAGCAGAATGGGTGCGAGATAAAGGACACCAGATAAAATCACAAGTATCAGCAGCTTCAGGTGCTGTAGCTTTAATTCAACTACAGGAAGATCTAAAGAAGTTGAACCAAGGTGAAAACAAGGAAGAGACCTTAATGAAAGCCATTGAAGAGAGGAAGGATCAAGTGCTTAATTCTCTATGGCAAATTAATGTCGTTGATATTGAGTCAACCTTATCACGTGTCTGCCAAGCAGTACTTAAAGACCCCACTGTGTCCAGGGATGTTCTGAGGGATCGGGCTAAAGCATTGCAAAAGTTAGGAACAGTCTTCCAAGGTGCGAAAGCTGCTTACAGTAGAGAGAACAGTCTTCGCCATGAAAACGACACCAAAATAGATTCTGCATCATCATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ-X

Protein Analysis

302

Amino Acids

33.94

Weight (kDa)

5.78

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AccB7I CCANNNNNTGG 1 cut(s) 37
AccI GTMKAC 1 cut(s) 129
AclWI GGATC 4 cut(s) 2, 59, 678, 792
AcsI RAATTY 3 cut(s) 25, 167, 382
AcuI CTGAAG 2 cut(s) 267, 552
AcvI CACGTG 1 cut(s) 731
AfaI GTAC 1 cut(s) 747
AfiI CCNNNNNNNGG 1 cut(s) 37
AflIII ACRYGT 1 cut(s) 730
AgsI TTSAA 7 cut(s) 176, 221, 227, 338, 590, 616, 659
AjnI CCWGG 2 cut(s) 30, 767
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 7 cut(s) 140, 203, 310, 464, 566, 581, 836
AluI AGCT 7 cut(s) 140, 203, 310, 464, 566, 581, 836
Alw26I GTCTC 2 cut(s) 227, 632
AlwI GGATC 4 cut(s) 2, 59, 678, 792
AlwNI CAGNNNCTG 2 cut(s) 37, 575
ApeKI GCWGC 7 cut(s) 17, 137, 410, 430, 464, 563, 836
ApoI RAATTY 3 cut(s) 25, 167, 382
AseI ATTAAT 1 cut(s) 699
AspS9I GGNCC 2 cut(s) 241, 282
AsuHPI GGTGA 2 cut(s) 431, 635
AvaII GGWCC 2 cut(s) 241, 282
BauI CACGAG 1 cut(s) 454
BbrPI CACGTG 1 cut(s) 731
BbsI GAAGAC 2 cut(s) 811, 851
BbvI GCAGC 7 cut(s) 4, 149, 417, 422, 451, 575, 823
BccI CCATC 1 cut(s) 344
BcgI CGANNNNNNTGC 1 cut(s) 882
BciT130I CCWGG 2 cut(s) 32, 769
BcoDI GTCTC 2 cut(s) 227, 632
BfmI CTRYAG 3 cut(s) 135, 576, 593
BglII AGATCT 1 cut(s) 601
BisI GCNGC 7 cut(s) 18, 138, 411, 431, 465, 564, 837
BlsI GCNGC 7 cut(s) 19, 139, 412, 432, 466, 565, 838
BmcAI AGTACT 1 cut(s) 747
Bme1390I CCNGG 2 cut(s) 32, 769
Bme18I GGWCC 2 cut(s) 241, 282
BmgT120I GGNCC 2 cut(s) 241, 282
BmiI GGNNCC 2 cut(s) 243, 504
BmrFI CCNGG 2 cut(s) 32, 769
BmrI ACTGGG 1 cut(s) 47
BmsI GCATC 2 cut(s) 141, 902
BmuI ACTGGG 1 cut(s) 47
BpiI GAAGAC 2 cut(s) 811, 851
Bsa29I ATCGAT 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 731
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 4 cut(s) 123, 619, 768, 823
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 797
BseBI CCWGG 2 cut(s) 32, 769
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 4 cut(s) 123, 619, 768, 823
BseGI GGATG 2 cut(s) 156, 778
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMI GCAATG 1 cut(s) 797
BseMII CTCAG 1 cut(s) 770
BseNI ACTGG 1 cut(s) 42
BseXI GCAGC 7 cut(s) 4, 149, 417, 422, 451, 575, 823
BshVI ATCGAT 1 cut(s) 6
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 2 cut(s) 227, 632
Bso31I GGTCTC 1 cut(s) 632
Bsp143I GATC 7 cut(s) 3, 7, 64, 328, 601, 670, 784
Bsp19I CCATGG 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 771
BspDI ATCGAT 1 cut(s) 6
BspLI GGNNCC 2 cut(s) 243, 504
BspMAI CTGCAG 1 cut(s) 139
BspPI GGATC 4 cut(s) 2, 59, 678, 792
BspTNI GGTCTC 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 797
BsrI ACTGG 1 cut(s) 42
BssECI CCNNGG 4 cut(s) 123, 619, 768, 823
BssMI GATC 7 cut(s) 3, 7, 64, 328, 601, 670, 784
BssSI CACGAG 1 cut(s) 454
BssT1I CCWWGG 3 cut(s) 123, 619, 823
Bst2BI CACGAG 1 cut(s) 454
Bst2UI CCWGG 2 cut(s) 32, 769
Bst4CI ACNGT 4 cut(s) 763, 817, 845, 857
Bst6I CTCTTC 3 cut(s) 225, 630, 654
BstBAI YACGTR 1 cut(s) 731
BstC8I GCNNGC 2 cut(s) 106, 462
BstDEI CTNAG 1 cut(s) 779
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 2 cut(s) 156, 778
BstKTI GATC 7 cut(s) 6, 10, 67, 331, 604, 673, 787
BstMAI GTCTC 2 cut(s) 227, 632
BstMBI GATC 7 cut(s) 3, 7, 64, 328, 601, 670, 784
BstMWI GCNNNNNNNGC 2 cut(s) 572, 836
BstNI CCWGG 2 cut(s) 32, 769
BstSCI CCNGG 2 cut(s) 30, 767
BstSFI CTRYAG 3 cut(s) 135, 576, 593
BstV1I GCAGC 7 cut(s) 4, 149, 417, 422, 451, 575, 823
BstV2I GAAGAC 2 cut(s) 811, 851
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
Bsu15I ATCGAT 1 cut(s) 6
BsuTUI ATCGAT 1 cut(s) 6
BtgI CCRYGG 1 cut(s) 123
BtsCI GGATG 2 cut(s) 156, 778
BtsIMutI CAGTG 2 cut(s) 35, 759
Cac8I GCNNGC 2 cut(s) 106, 462
CaiI CAGNNNCTG 2 cut(s) 37, 575
Cfr13I GGNCC 2 cut(s) 241, 282
ClaI ATCGAT 1 cut(s) 6
Csp6I GTAC 1 cut(s) 746
CviAII CATG 3 cut(s) 124, 496, 866
CviQI GTAC 1 cut(s) 746
DdeI CTNAG 1 cut(s) 779
DpnI GATC 7 cut(s) 5, 9, 66, 330, 603, 672, 786
DpnII GATC 7 cut(s) 3, 7, 64, 328, 601, 670, 784
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
Eam1104I CTCTTC 3 cut(s) 225, 630, 654
EarI CTCTTC 3 cut(s) 225, 630, 654
Eco130I CCWWGG 3 cut(s) 123, 619, 823
Eco31I GGTCTC 1 cut(s) 632
Eco47I GGWCC 2 cut(s) 241, 282
Eco57I CTGAAG 2 cut(s) 267, 552
Eco72I CACGTG 1 cut(s) 731
EcoO109I RGGNCCY 2 cut(s) 241, 282
EcoRI GAATTC 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 30, 767
EcoT14I CCWWGG 3 cut(s) 123, 619, 823
ErhI CCWWGG 3 cut(s) 123, 619, 823
FaeI CATG 3 cut(s) 127, 499, 869
FalI AAGNNNNNCTT 4 cut(s) 318, 350, 732, 764
FatI CATG 3 cut(s) 123, 495, 865
FauNDI CATATG 1 cut(s) 85
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 7 cut(s) 18, 138, 411, 431, 465, 564, 837
FokI GGATG 2 cut(s) 163, 785
Fsp4HI GCNGC 7 cut(s) 18, 138, 411, 431, 465, 564, 837
GluI GCNGC 7 cut(s) 18, 138, 411, 431, 465, 564, 837
Hin1II CATG 3 cut(s) 127, 499, 869
HincII GTYRAC 2 cut(s) 191, 720
HindII GTYRAC 2 cut(s) 191, 720
HinfI GANTC 3 cut(s) 119, 716, 887
HphI GGTGA 2 cut(s) 431, 635
Hpy166II GTNNAC 3 cut(s) 130, 191, 720
Hpy188I TCNGA 3 cut(s) 324, 388, 780
Hpy188III TCNNGA 1 cut(s) 68
Hpy8I GTNNAC 3 cut(s) 130, 191, 720
HpyAV CCTTC 4 cut(s) 286, 344, 493, 661
HpyCH4III ACNGT 4 cut(s) 763, 817, 845, 857
HpyCH4IV ACGT 1 cut(s) 730
HpyCH4V TGCA 6 cut(s) 137, 289, 433, 467, 802, 893
HpyF10VI GCNNNNNNNGC 2 cut(s) 572, 836
HpyF3I CTNAG 1 cut(s) 779
HpySE526I ACGT 1 cut(s) 730
Hsp92II CATG 3 cut(s) 127, 499, 869
Kzo9I GATC 7 cut(s) 3, 7, 64, 328, 601, 670, 784
LmnI GCTCC 1 cut(s) 162
Lsp1109I GCAGC 7 cut(s) 4, 149, 417, 422, 451, 575, 823
LweI GCATC 2 cut(s) 141, 902
MaeII ACGT 1 cut(s) 730
MaeIII GTNAC 1 cut(s) 265
MalI GATC 7 cut(s) 5, 9, 66, 330, 603, 672, 786
MboI GATC 7 cut(s) 3, 7, 64, 328, 601, 670, 784
MboII GAAGA 8 cut(s) 188, 239, 242, 611, 647, 671, 811, 851
MflI RGATCY 1 cut(s) 601
MluCI AATT 8 cut(s) 25, 167, 222, 382, 474, 585, 682, 696
MlyI GAGTC 1 cut(s) 725
MnlI CCTC 7 cut(s) 37, 273, 410, 415, 450, 657, 774
MseI TTAA 6 cut(s) 59, 584, 644, 681, 699, 750
MspA1I CMGCKG 1 cut(s) 140
MspR9I CCNGG 2 cut(s) 32, 769
MvaI CCWGG 2 cut(s) 32, 769
MwoI GCNNNNNNNGC 2 cut(s) 572, 836
NcoI CCATGG 1 cut(s) 123
NdeI CATATG 1 cut(s) 85
NdeII GATC 7 cut(s) 3, 7, 64, 328, 601, 670, 784
NlaIII CATG 3 cut(s) 127, 499, 869
NlaIV GGNNCC 2 cut(s) 243, 504
NmuCI GTSAC 1 cut(s) 265
PfeI GAWTC 2 cut(s) 119, 887
PflMI CCANNNNNTGG 1 cut(s) 37
PkrI GCNGC 7 cut(s) 19, 139, 412, 432, 466, 565, 838
PleI GAGTC 1 cut(s) 724
PmaCI CACGTG 1 cut(s) 731
PmlI CACGTG 1 cut(s) 731
PpsI GAGTC 1 cut(s) 724
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 2 cut(s) 241, 282
PshBI ATTAAT 1 cut(s) 699
Psp5II RGGWCCY 2 cut(s) 241, 282
Psp6I CCWGG 2 cut(s) 30, 767
PspCI CACGTG 1 cut(s) 731
PspGI CCWGG 2 cut(s) 30, 767
PspN4I GGNNCC 2 cut(s) 243, 504
PspPI GGNCC 2 cut(s) 241, 282
PspPPI RGGWCCY 2 cut(s) 241, 282
PstI CTGCAG 1 cut(s) 139
PstNI CAGNNNCTG 2 cut(s) 37, 575
PsuI RGATCY 1 cut(s) 601
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 1 cut(s) 747
RsaNI GTAC 1 cut(s) 746
SaqAI TTAA 6 cut(s) 59, 584, 644, 681, 699, 750
SatI GCNGC 7 cut(s) 18, 138, 411, 431, 465, 564, 837
Sau3AI GATC 7 cut(s) 3, 7, 64, 328, 601, 670, 784
Sau96I GGNCC 2 cut(s) 241, 282
ScaI AGTACT 1 cut(s) 747
SchI GAGTC 1 cut(s) 725
ScrFI CCNGG 2 cut(s) 32, 769
SfaNI GCATC 2 cut(s) 141, 902
SfcI CTRYAG 3 cut(s) 135, 576, 593
SinI GGWCC 2 cut(s) 241, 282
Sse9I AATT 8 cut(s) 25, 167, 222, 382, 474, 585, 682, 696
StyD4I CCNGG 2 cut(s) 30, 767
StyI CCWWGG 3 cut(s) 123, 619, 823
TaaI ACNGT 4 cut(s) 763, 817, 845, 857
TaiI ACGT 1 cut(s) 733
TaqI TCGA 1 cut(s) 6
TasI AATT 8 cut(s) 25, 167, 222, 382, 474, 585, 682, 696
TatI WGTACW 1 cut(s) 745
TfiI GAWTC 2 cut(s) 119, 887
Tru1I TTAA 6 cut(s) 59, 584, 644, 681, 699, 750
Tru9I TTAA 6 cut(s) 59, 584, 644, 681, 699, 750
TscAI CASTG 2 cut(s) 42, 766
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 7 cut(s) 17, 137, 410, 430, 464, 563, 836
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 3 cut(s) 512, 662, 882
TspGWI ACGGA 3 cut(s) 111, 242, 284
TspRI CASTG 2 cut(s) 42, 766
Van91I CCANNNNNTGG 1 cut(s) 37
VpaK11BI GGWCC 2 cut(s) 241, 282
VspI ATTAAT 1 cut(s) 699
XapI RAATTY 3 cut(s) 25, 167, 382
XmiI GTMKAC 1 cut(s) 129
ZrmI AGTACT 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.