Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0004488

Physical Location & Seq

Genomic Coordinates Forward (+)

9157568 .. 9167833

Gene Structure 10266 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3354 bp

ATGGAAAATAGTTTCAACTTACTAAATTTCATTATTTCCCTCGCAATCCAAACCACAAAAGTTATGGGGGGAGCAACTCTGCTTCGCTCTTCTCTCGCCTCCAGCAATCGCCTCTTCTTCTCCTTCCGCTCTTCCCGCCGCCTCTCCTGTTTTTCCCTCCCCTCATCTCGCTCTTCATCTTCTTCTTCAGCTTCTTCATCTTCTTCTTCAGCTTCTGCTGTAAGAAACCACTGCCGCCGTCCGATTCTCAACCCCAGCAACCCCAGCAGACGCTCTCTGCTCCGCCCCGCCAGCAGATTACTCCCCTCTTCTGCCCCCAACGTCGCTAGAAGCTTCTCCTCCCTCTCTGCGCGCGCAGTCGCCATTCCATTCACTCAGTCCCCTTCAGAGTATTCTGGGGTGCAGGATGACGTCGTGGAAAAGCTCGGGTTCGAGAAGGTCTCTGAGGAGTTCATTGGAGAGTGTAAATCCAAAGCTCTGCTCTTCAGGCACAAGAAGACCGGTGCCCAAGTTATTTCGGTGTCCAATGATGACGAGAATAAGGTTTTCGGCATTGTTTTTCGGACCCCGCCGAACGATTCCACTGGGATCCCACACATCCTTGAACACAGTGTATTGTGTGGGTCAAGAAAGTACCCTTTGAAAGAACCATTTGTTGAGTTGTTAAAAGGGAGCTTGAATACGTTTCTGAATGCATTTACTTATCCGGATAGAACTTGCTACCCGGTTGCTTCCACAAATACAAAGGATTTCTATAACCTTGTCGATGTATATCTGGATGCTGTCTTCTTTCCTAAATGTGTGGAGGACGTCAGGACTTTCCAGCAGGAGGGTTGGCATTACGAGCTCAATGATCCTTCTGATGATATATCGTATAAAGGAGTTGTTTTTAATGAGATGAAAGGGGTCTATTCCCAGCCTGATAATATACTCGGGCGGACTGCTCAACAGGCTCTTTTTCCGGATAACACCTATGCTGTTGACAGTGGGGGTGATCCAACCGTTATTCCTAACCTGACATTTGACGAATTCAAGGAGTTCCACCGTAAGTATTACCATCCCAGCAATGCCCGAATATGGTTTTATGGAGATGATGATCCAACTGAACGCCTGCGCATTTTGAGTGAGTACCTAGATATGTTCGATGCAAGTTCATCTCCAAATGAATCAAGAATTCAACCGCAGAAACTATTTTCAGAACCAATTAGGATTTCTGAGAAATATCCTGCTGGTGAAGGTGGTGACTTGAGGAAGAAAAACATGGTATGCCTCAATTGGTTGCTTGCTGATAAGCCCCTAGATTTGGAGACTGAGCTTACGCTTGGGTTTCTGGATCATCTTATGCTGGGAACGCCTGCTTCTCCATTGAGGAAAATATTGCTTGAGAGTGGGTTGGGTGAGGCCATCGTTGGTGGTGGAGTTGACGATGATCTTCTTCAACCTCTATTTAGTATTGGTTTGAAGGGTGTTTCAGAAGATGATATTCAAAAGGTTGAAGAAGTTGTCATGAGTACACTTAAAAAGTTAGCAGAGGAAGGTTTTTATACGGAAGCTGTAGAAGCATCCATGAATACGATTGAGTTCTCTCTGAGGGAAAACAACACGGGATCATTTCCTCGTGGCTTGTCACTAATGCTCCGATCCATAGGTAAATGGATATATGATTTGGATCCGTTTGAGCCATTAAAGTATGAGAAACCTTTGCTGGCACTGAAAGCCAGAATAGAAGCTGAAGGCTCTAAGGCTGTTTTCTCTCCCTTAATCAAGAAATTCATTCTGGACAATCCCCATCGAGTTGTGGTAGAAATGCAGCCTGATCCAGAGAAAGCTTCTCGTGATGAAGAGGCTGAGAAAGAAATATTGCATAAAGTTAAATCCTGTATGACAGAAGAAGATCTAGCTGAGCTAGCACGTGCTACACAGGAGCTGCGACGGAGGCAGGAAACTCCTGACCCTTCGGAAGCTTTGAAAAGTGTTCCAAGTCTCTCTTTGCAAGACATTCCAAAGGAACCTACTTGTATTCCTACAGAGGTTGGGGATGTCAATGGTGTGAAAGTTCTGCGGCATGACCTCTTCACAAATGATGTCCTGTACACTGAAGTTGTGTTCAATATGAGTTCACTAAAGCAAGAGCTACTTCCTTTGGTACCCCTTTTTTGCCAATCGTTGCTAGAGATGGGTACAAAAGATTTGAGTTTTGTGCAACTGAATCAGTTGATCGGAAGGAAAACCGGAGGAATATCAGTTTATCCAATGACATCATCTGTACGGGGCAAGGAGGATCCCTGCAGCCATATAATTGTTCGTGGAAAAACTATGGCAGGACGTGCTGATGATCTATTTCACCTGTTCAACTGTGTTCTTCAAGAAGTCCAGTTTACAGACCAGCAGCGGTTTAAGCAGTTTGTCTCCCAAAGCAAAGCAAGAATGGAGAACCGATTGAGAGGTAGTGGTCATGGAATTGTTGCTGCAAGGATGGATGCAAAGCTAAATACTGCGGGGTGGATTTCTGAACAAATGGGTGGTGTCAGTTACTTGGAGTTTCTTCAAGCTCTTGAAGAGAAAGTTGATCAAGATTGGGATGGAATTTCTTCATCTCTTGAGGAGATCCGCAAGTCCTTGCTTTCTAGGCAAGGTTGCATAGTAAATGTCACTGCTGAAGGGAAGAATCTAACCAACTCTGAGAAGTTTGTGAGTAAATTTCTTGATTTGCTGCCTAACAGCTCTCCTGTTGCGGCTGCTACTTGGAATGCTCAATTACCTTCAGCAAATGAAGCCATTGTAATCCCTACTCAGGTTAATTATATTGGAAAAGCTGGTAACATTTATGACACTGGTTATCGGCTTAGTGGGAGTGCACATGTTATCTCCACCTACATTAGTAATACATGGTTGTGGGATCGTGTAAGAGTTAGTGGTGGAGCATATGGAGGATTTTGTGACTTTGATAATCACTCAGGAGTATTCTCGTTCTTATCTTATCGGGACCCCAACTTGTTAAAGACACTTGATGTATATGATGGGACTGGGGATTTTCTTCGACAATTAGAAATCGATGAAGAGACTCTTACCAAGTCCATCATTGGGACAATCGGAGATGTTGATTCGTACCAGCTTCCTGATGCCAAAGGTTATAGTAGTTTGTTGCGATATTTATTGGGAATCACAGAAGAAGAAAGGCAAATAAGACGAGCAGAGATACTGTCAACAAGCTTGAAGGACTTCAAGGAATTTGCAAATGCGATCGACGTAGTCAAAGACAAAGGGGTTGTGGTTGCAGTGGCATCTCCAGATGACGTCGATGCAGCACAAAAGGAGCGAAACGACTTCTTTCAAGTAAAGAAAGCCCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031967 GO:0031974 GO:0031975 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

1118

Amino Acids

124.49

Weight (kDa)

5.39

Isoelectric Point (pI)

46.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	191 - 267	3.4e-06	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	338 - 524	3.1e-27	Peptidase M16 inactive domain
M16C_assoc	PF08367	603 - 852	4.5e-76	Peptidase M16C associated
PreP_C	PF22516	924 - 1096	6.2e-82	PreP C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT3G19170 AT3G19170
fragaria_vesca	FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380
malus_domestica	MD05G1078000.v1.1 MD10G1092200.v1.1 MD11G1150900.v1.1
prunus_persica	Prupe.8G122100_v2.0.a1 Prupe.8G122200_v2.0.a1
pyrus_communis	pycom05g07050 pycom05g07160 pycom10g07580
rosa_chinensis	RchiOBHm_Chr6g0258261
rosa_laevigata	RLG00000014634
rosa_multiflora	Rmu_sc0004230.1_g000015
rosa_roxburghii	Rroxscaffold_178G00437220 Rroxscaffold_7G00205380
rosa_rugosa	Rorug05G0579700
rosa_samantha	Rh6AG097600 Rh6BG088700 Rh6CG084700 Rh6DG080800
rosa_wichuraiana	Rw6G008290 Rw6G008350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	3 cut(s)	414, 813, 3300
Acc16I	TGCGCA	1 cut(s)	1115
Acc65I	GGTACC	1 cut(s)	2146
AccB1I	GGYRCC	2 cut(s)	503, 2146
AccBSI	CCGCTC	1 cut(s)	129
AccII	CGCG	2 cut(s)	352, 354
AccIII	TCCGGA	2 cut(s)	706, 961
AcsI	RAATTY	7 cut(s)	25, 1028, 1173, 1769, 2586, 2699, 3230
AcuI	CTGAAG	8 cut(s)	171, 192, 369, 469, 1752, 2118, 2679, 2748
AcvI	CACGTG	1 cut(s)	1913
AcyI	GRCGYC	3 cut(s)	411, 810, 3297
AfaI	GTAC	8 cut(s)	635, 1130, 1513, 2093, 2148, 2182, 2268, 3110
AfiI	CCNNNNNNNGG	5 cut(s)	829, 1077, 1303, 2794, 3084
AflIII	ACRYGT	1 cut(s)	2860
AgeI	ACCGGT	1 cut(s)	500
AjiI	CACGTC	1 cut(s)	2327
AjuI	GAANNNNNNNTTGG	2 cut(s)	1195, 1227
Alw21I	GWGCWC	2 cut(s)	849, 2860
Alw26I	GTCTC	5 cut(s)	445, 1301, 1988, 2413, 3056
Alw44I	GTGCAC	1 cut(s)	2856
AlwNI	CAGNNNCTG	2 cut(s)	215, 1927
Ama87I	CYCGRG	2 cut(s)	425, 932
Aor13HI	TCCGGA	2 cut(s)	706, 961
AoxI	GGCC	1 cut(s)	1401
ApaLI	GTGCAC	1 cut(s)	2856
ApeKI	GCWGC	8 cut(s)	1810, 1927, 2289, 2389, 2468, 2713, 2738, 3305
ApoI	RAATTY	7 cut(s)	25, 1028, 1173, 1769, 2586, 2699, 3230
AsiGI	ACCGGT	1 cut(s)	500
Asp700I	GAANNNNTTC	2 cut(s)	2590, 3221
Asp718I	GGTACC	1 cut(s)	2146
AspLEI	GCGC	4 cut(s)	352, 354, 356, 1116
AspS9I	GGNCC	2 cut(s)	564, 2986
AsuC2I	CCSGG	1 cut(s)	725
AsuHPI	GGTGA	5 cut(s)	1004, 1244, 1253, 1409, 2336
AsuNHI	GCTAGC	1 cut(s)	1906
AvaI	CYCGRG	2 cut(s)	425, 932
AvaII	GGWCC	2 cut(s)	564, 2986
BaeGI	GKGCMC	2 cut(s)	508, 2860
BamHI	GGATCC	3 cut(s)	588, 1669, 2281
BanI	GGYRCC	2 cut(s)	503, 2146
BanII	GRGCYC	1 cut(s)	849
BauI	CACGAG	2 cut(s)	1617, 1833
BbrPI	CACGTG	1 cut(s)	1913
BbsI	GAAGAC	2 cut(s)	503, 778
Bbv12I	GWGCWC	2 cut(s)	849, 2860
BbvI	GCAGC	8 cut(s)	1822, 1914, 2301, 2401, 2455, 2700, 2725, 3317
BccI	CCATC	8 cut(s)	1065, 1412, 1797, 2170, 2470, 2576, 3014, 3086
BceAI	ACGGC	1 cut(s)	222
BclI	TGATCA	1 cut(s)	2569
BcnI	CCSGG	1 cut(s)	725
BcoDI	GTCTC	5 cut(s)	445, 1301, 1988, 2413, 3056
BfaI	CTAG	8 cut(s)	327, 1133, 1298, 1898, 1907, 2171, 2628, 3352
BfmI	CTRYAG	3 cut(s)	1554, 2025, 2287
BglII	AGATCT	1 cut(s)	1894
BlpI	GCTNAGC	1 cut(s)	1902
Bme1390I	CCNGG	1 cut(s)	725
Bme18I	GGWCC	2 cut(s)	564, 2986
BmeT110I	CYCGRG	2 cut(s)	425, 932
BmgBI	CACGTC	1 cut(s)	2327
BmgT120I	GGNCC	2 cut(s)	564, 2986
BmiI	GGNNCC	9 cut(s)	505, 566, 590, 1671, 2010, 2148, 2283, 2987, 2988
BmrFI	CCNGG	1 cut(s)	725
BmrI	ACTGGG	2 cut(s)	594, 3036
BmsI	GCATC	7 cut(s)	769, 1135, 1571, 2470, 3112, 3292, 3293
BmtI	GCTAGC	1 cut(s)	1910
BmuI	ACTGGG	2 cut(s)	594, 3036
BpiI	GAAGAC	2 cut(s)	503, 778
BplI	GAGNNNNNCTC	2 cut(s)	425, 457
BpmI	CTGGAG	2 cut(s)	85, 3273
Bpu1102I	GCTNAGC	1 cut(s)	1902
BpuEI	CTTGAG	3 cut(s)	1267, 1403, 2621
BpuMI	CCSGG	1 cut(s)	725
Bsa29I	ATCGAT	1 cut(s)	3054
BsaAI	YACGTR	1 cut(s)	1913
BsaBI	GATNNNNATC	3 cut(s)	771, 1095, 1668
BsaHI	GRCGYC	3 cut(s)	411, 810, 3297
BsaI	GGTCTC	1 cut(s)	445
BsaWI	WCCGGW	4 cut(s)	500, 706, 961, 2231
BsaXI	ACNNNNNCTCC	2 cut(s)	1620, 1650
Bsc4I	CCNNNNNNNGG	5 cut(s)	829, 1077, 1303, 2794, 3084
Bse118I	RCCGGY	1 cut(s)	500
Bse1I	ACTGG	4 cut(s)	589, 2374, 2839, 3031
Bse3DI	GCAATG	1 cut(s)	1072
Bse8I	GATNNNNATC	3 cut(s)	771, 1095, 1668
BseAI	TCCGGA	2 cut(s)	706, 961
BseCI	ATCGAT	1 cut(s)	3054
BseGI	GGATG	9 cut(s)	412, 597, 784, 1057, 1562, 2044, 2481, 2485, 2587
BseJI	GATNNNNATC	3 cut(s)	771, 1095, 1668
BseLI	CCNNNNNNNGG	5 cut(s)	829, 1077, 1303, 2794, 3084
BseMI	GCAATG	1 cut(s)	1072
BseNI	ACTGG	4 cut(s)	589, 2374, 2839, 3031
BsePI	GCGCGC	2 cut(s)	350, 352
BseRI	GAGGAG	3 cut(s)	328, 461, 2618
BseSI	GKGCMC	2 cut(s)	508, 2860
BseXI	GCAGC	8 cut(s)	1822, 1914, 2301, 2401, 2455, 2700, 2725, 3317
BseYI	CCCAGC	5 cut(s)	254, 263, 915, 1061, 1345
BsgI	GTGCAG	1 cut(s)	422
Bsh1236I	CGCG	2 cut(s)	352, 354
Bsh1285I	CGRYCG	1 cut(s)	3246
BshFI	GGCC	1 cut(s)	1403
BshNI	GGYRCC	2 cut(s)	503, 2146
BshTI	ACCGGT	1 cut(s)	500
BshVI	ATCGAT	1 cut(s)	3054
BsiEI	CGRYCG	1 cut(s)	3246
BsiHKAI	GWGCWC	2 cut(s)	849, 2860
BsiHKCI	CYCGRG	2 cut(s)	425, 932
BsiSI	CCGG	5 cut(s)	501, 707, 725, 962, 2232
BslFI	GGGAC	4 cut(s)	364, 2999, 3037, 3100
BslI	CCNNNNNNNGG	5 cut(s)	829, 1077, 1303, 2794, 3084
BsmAI	GTCTC	5 cut(s)	445, 1301, 1988, 2413, 3056
BsmFI	GGGAC	4 cut(s)	364, 2999, 3037, 3100
BsmI	GAATGC	2 cut(s)	697, 2755
BsnI	GGCC	1 cut(s)	1403
Bso31I	GGTCTC	1 cut(s)	445
BsoBI	CYCGRG	2 cut(s)	425, 932
Bsp1286I	GDGCHC	3 cut(s)	508, 849, 2860
Bsp13I	TCCGGA	2 cut(s)	706, 961
Bsp1407I	TGTACA	1 cut(s)	2091
Bsp1720I	GCTNAGC	1 cut(s)	1902
BspANI	GGCC	1 cut(s)	1403
BspDI	ATCGAT	1 cut(s)	3054
BspEI	TCCGGA	2 cut(s)	706, 961
BspFNI	CGCG	2 cut(s)	352, 354
BspHI	TCATGA	1 cut(s)	1506
BspLI	GGNNCC	9 cut(s)	505, 566, 590, 1671, 2010, 2148, 2283, 2987, 2988
BspMAI	CTGCAG	1 cut(s)	2291
BspOI	GCTAGC	1 cut(s)	1910
BspQI	GCTCTTC	4 cut(s)	94, 136, 178, 488
BspT107I	GGYRCC	2 cut(s)	503, 2146
BspTNI	GGTCTC	1 cut(s)	445
BsrBI	CCGCTC	1 cut(s)	129
BsrDI	GCAATG	1 cut(s)	1072
BsrFI	RCCGGY	1 cut(s)	500
BsrGI	TGTACA	1 cut(s)	2091
BsrI	ACTGG	4 cut(s)	589, 2374, 2839, 3031
BssAI	RCCGGY	1 cut(s)	500
BssHII	GCGCGC	2 cut(s)	350, 352
BssNI	GRCGYC	3 cut(s)	411, 810, 3297
BssSI	CACGAG	2 cut(s)	1617, 1833
Bst2BI	CACGAG	2 cut(s)	1617, 1833
Bst4CI	ACNGT	6 cut(s)	611, 986, 1003, 1046, 2357, 3204
BstACI	GRCGYC	3 cut(s)	411, 810, 3297
BstAPI	GCANNNNNTGC	1 cut(s)	2327
BstAUI	TGTACA	1 cut(s)	2091
BstBAI	YACGTR	1 cut(s)	1913
BstC8I	GCNNGC	8 cut(s)	292, 352, 354, 1112, 1284, 1356, 1707, 1908
BstF5I	GGATG	9 cut(s)	412, 597, 784, 1057, 1562, 2044, 2481, 2485, 2587
BstFNI	CGCG	2 cut(s)	352, 354
BstHHI	GCGC	4 cut(s)	352, 354, 356, 1116
BstMAI	GTCTC	5 cut(s)	445, 1301, 1988, 2413, 3056
BstMCI	CGRYCG	1 cut(s)	3246
BstNSI	RCATGY	1 cut(s)	2864
BstSCI	CCNGG	1 cut(s)	723
BstSFI	CTRYAG	3 cut(s)	1554, 2025, 2287
BstSLI	GKGCMC	2 cut(s)	508, 2860
BstUI	CGCG	2 cut(s)	352, 354
BstV1I	GCAGC	8 cut(s)	1822, 1914, 2301, 2401, 2455, 2700, 2725, 3317
BstV2I	GAAGAC	2 cut(s)	503, 778
BstX2I	RGATCY	5 cut(s)	588, 1669, 1894, 2281, 2607
BstYI	RGATCY	5 cut(s)	588, 1669, 1894, 2281, 2607
Bsu15I	ATCGAT	1 cut(s)	3054
BsuRI	GGCC	1 cut(s)	1403
BsuTUI	ATCGAT	1 cut(s)	3054
BtrI	CACGTC	1 cut(s)	2327
BtsCI	GGATG	9 cut(s)	412, 597, 784, 1057, 1562, 2044, 2481, 2485, 2587
BtsI	GCAGTG	3 cut(s)	229, 2652, 3285
BtsIMutI	CAGTG	9 cut(s)	229, 582, 616, 991, 1709, 2094, 2652, 2832, 3285
Cac8I	GCNNGC	8 cut(s)	292, 352, 354, 1112, 1284, 1356, 1707, 1908
CaiI	CAGNNNCTG	2 cut(s)	215, 1927
CciI	TCATGA	1 cut(s)	1506
CfoI	GCGC	4 cut(s)	352, 354, 356, 1116
Cfr10I	RCCGGY	1 cut(s)	500
Cfr13I	GGNCC	2 cut(s)	564, 2986
ClaI	ATCGAT	1 cut(s)	3054
CseI	GACGC	1 cut(s)	279
Csp6I	GTAC	8 cut(s)	634, 1129, 1512, 2092, 2147, 2181, 2267, 3109
CspAI	ACCGGT	1 cut(s)	500
CviAII	CATG	7 cut(s)	1261, 1507, 1567, 2066, 2456, 2861, 2889
CviQI	GTAC	8 cut(s)	634, 1129, 1512, 2092, 2147, 2181, 2267, 3109
EciI	GGCGGA	2 cut(s)	272, 952
Ecl136II	GAGCTC	1 cut(s)	847
Eco24I	GRGCYC	1 cut(s)	849
Eco31I	GGTCTC	1 cut(s)	445
Eco47I	GGWCC	2 cut(s)	564, 2986
Eco53kI	GAGCTC	1 cut(s)	847
Eco57I	CTGAAG	8 cut(s)	171, 192, 369, 469, 1752, 2118, 2679, 2748
Eco72I	CACGTG	1 cut(s)	1913
Eco88I	CYCGRG	2 cut(s)	425, 932
EcoICRI	GAGCTC	1 cut(s)	847
EcoO109I	RGGNCCY	1 cut(s)	2986
EcoRI	GAATTC	2 cut(s)	1028, 1173
EcoT22I	ATGCAT	1 cut(s)	697
EcoT38I	GRGCYC	1 cut(s)	849
FaeI	CATG	7 cut(s)	1264, 1510, 1570, 2069, 2459, 2864, 2892
FalI	AAGNNNNNCTT	6 cut(s)	1972, 2004, 2121, 2153, 3051, 3083
FaqI	GGGAC	4 cut(s)	364, 2999, 3037, 3100
FatI	CATG	7 cut(s)	1260, 1506, 1566, 2065, 2455, 2860, 2888
FauI	CCCGC	4 cut(s)	143, 295, 576, 2491
FauNDI	CATATG	1 cut(s)	2926
FbaI	TGATCA	1 cut(s)	2569
FokI	GGATG	9 cut(s)	419, 584, 791, 1044, 1549, 2051, 2488, 2492, 2594
FriOI	GRGCYC	1 cut(s)	849
FspBI	CTAG	8 cut(s)	327, 1133, 1298, 1898, 1907, 2171, 2628, 3352
FspI	TGCGCA	1 cut(s)	1115
GlaI	GCGC	4 cut(s)	351, 353, 355, 1115
GsaI	CCCAGC	5 cut(s)	258, 267, 919, 1065, 1349
GsuI	CTGGAG	2 cut(s)	85, 3273
HaeIII	GGCC	1 cut(s)	1403
HapII	CCGG	5 cut(s)	501, 707, 725, 962, 2232
HgaI	GACGC	1 cut(s)	279
HhaI	GCGC	4 cut(s)	352, 354, 356, 1116
Hin1I	GRCGYC	3 cut(s)	411, 810, 3297
Hin1II	CATG	7 cut(s)	1264, 1510, 1570, 2069, 2459, 2864, 2892
Hin6I	GCGC	4 cut(s)	350, 352, 354, 1114
HinP1I	GCGC	4 cut(s)	350, 352, 354, 1114
HincII	GTYRAC	3 cut(s)	982, 1423, 3207
HindII	GTYRAC	3 cut(s)	982, 1423, 3207
HindIII	AAGCTT	4 cut(s)	331, 1827, 1962, 3211
HinfI	GANTC	8 cut(s)	244, 578, 1166, 2209, 2668, 3064, 3104, 3162
HpaII	CCGG	5 cut(s)	501, 707, 725, 962, 2232
HphI	GGTGA	5 cut(s)	1004, 1244, 1253, 1409, 2336
Hpy166II	GTNNAC	8 cut(s)	982, 1423, 1514, 2094, 2120, 2379, 2858, 3207
Hpy8I	GTNNAC	8 cut(s)	982, 1423, 1514, 2094, 2120, 2379, 2858, 3207
Hpy99I	CGWCG	5 cut(s)	326, 416, 1935, 3251, 3302
HpyCH4III	ACNGT	6 cut(s)	611, 986, 1003, 1046, 2357, 3204
HpyCH4IV	ACGT	8 cut(s)	321, 411, 683, 810, 1912, 2326, 3249, 3297
HpySE526I	ACGT	8 cut(s)	321, 411, 683, 810, 1912, 2326, 3249, 3297
Hsp92I	GRCGYC	3 cut(s)	411, 810, 3297
Hsp92II	CATG	7 cut(s)	1264, 1510, 1570, 2069, 2459, 2864, 2892
HspAI	GCGC	4 cut(s)	350, 352, 354, 1114
KflI	GGGWCCC	1 cut(s)	2986
Kpn2I	TCCGGA	2 cut(s)	706, 961
KpnI	GGTACC	1 cut(s)	2150
Ksp22I	TGATCA	1 cut(s)	2569
LguI	GCTCTTC	4 cut(s)	94, 136, 178, 488
LmnI	GCTCC	7 cut(s)	71, 285, 672, 1641, 1924, 2921, 3316
Lsp1109I	GCAGC	8 cut(s)	1822, 1914, 2301, 2401, 2455, 2700, 2725, 3317
LweI	GCATC	7 cut(s)	769, 1135, 1571, 2470, 3112, 3292, 3293
MaeI	CTAG	8 cut(s)	327, 1133, 1298, 1898, 1907, 2171, 2628, 3352
MaeII	ACGT	8 cut(s)	321, 411, 683, 810, 1912, 2326, 3249, 3297
MaeIII	GTNAC	6 cut(s)	1241, 1626, 2531, 2650, 2819, 2939
MbiI	CCGCTC	1 cut(s)	129
MfeI	CAATTG	1 cut(s)	1273
MflI	RGATCY	5 cut(s)	588, 1669, 1894, 2281, 2607
MhlI	GDGCHC	3 cut(s)	508, 849, 2860
MlyI	GAGTC	1 cut(s)	3058
MmeI	TCCRAC	2 cut(s)	1022, 1124
Mph1103I	ATGCAT	1 cut(s)	697
MroI	TCCGGA	2 cut(s)	706, 961
MroXI	GAANNNNTTC	2 cut(s)	2590, 3221
MseI	TTAA	9 cut(s)	665, 891, 1518, 1685, 1760, 1872, 2397, 2799, 2999
MslI	CAYNNNNRTG	1 cut(s)	2893
MspA1I	CMGCKG	1 cut(s)	2392
MspI	CCGG	5 cut(s)	501, 707, 725, 962, 2232
MspR9I	CCNGG	1 cut(s)	725
MunI	CAATTG	1 cut(s)	1273
Mva1269I	GAATGC	2 cut(s)	697, 2755
MvnI	CGCG	2 cut(s)	352, 354
NciI	CCSGG	1 cut(s)	725
NdeI	CATATG	1 cut(s)	2926
NheI	GCTAGC	1 cut(s)	1906
NlaIII	CATG	7 cut(s)	1264, 1510, 1570, 2069, 2459, 2864, 2892
NlaIV	GGNNCC	9 cut(s)	505, 566, 590, 1671, 2010, 2148, 2283, 2987, 2988
NmuCI	GTSAC	4 cut(s)	1241, 1626, 2650, 2939
NsbI	TGCGCA	1 cut(s)	1115
NsiI	ATGCAT	1 cut(s)	697
NspI	RCATGY	1 cut(s)	2864
PagI	TCATGA	1 cut(s)	1506
PauI	GCGCGC	2 cut(s)	350, 352
PciI	ACATGT	1 cut(s)	2860
PciSI	GCTCTTC	4 cut(s)	94, 136, 178, 488
PctI	GAATGC	2 cut(s)	697, 2755
PdmI	GAANNNNTTC	2 cut(s)	2590, 3221
PfeI	GAWTC	7 cut(s)	244, 578, 1166, 2209, 2668, 3104, 3162
PflFI	GACNNNGTC	1 cut(s)	3251
PinAI	ACCGGT	1 cut(s)	500
Ple19I	CGATCG	1 cut(s)	3246
PleI	GAGTC	1 cut(s)	3058
PmaCI	CACGTG	1 cut(s)	1913
PmlI	CACGTG	1 cut(s)	1913
PpsI	GAGTC	1 cut(s)	3058
Ppu21I	YACGTR	1 cut(s)	1913
PpuMI	RGGWCCY	1 cut(s)	2986
PscI	ACATGT	1 cut(s)	2860
Psp124BI	GAGCTC	1 cut(s)	849
Psp5II	RGGWCCY	1 cut(s)	2986
PspCI	CACGTG	1 cut(s)	1913
PspFI	CCCAGC	5 cut(s)	254, 263, 915, 1061, 1345
PspN4I	GGNNCC	9 cut(s)	505, 566, 590, 1671, 2010, 2148, 2283, 2987, 2988
PspPI	GGNCC	2 cut(s)	564, 2986
PspPPI	RGGWCCY	1 cut(s)	2986
PstI	CTGCAG	1 cut(s)	2291
PstNI	CAGNNNCTG	2 cut(s)	215, 1927
PsuI	RGATCY	5 cut(s)	588, 1669, 1894, 2281, 2607
PsyI	GACNNNGTC	1 cut(s)	3251
PteI	GCGCGC	2 cut(s)	350, 352
PvuI	CGATCG	1 cut(s)	3246
RsaI	GTAC	8 cut(s)	635, 1130, 1513, 2093, 2148, 2182, 2268, 3110
RsaNI	GTAC	8 cut(s)	634, 1129, 1512, 2092, 2147, 2181, 2267, 3109
RseI	CAYNNNNRTG	1 cut(s)	2893
SacI	GAGCTC	1 cut(s)	849
SapI	GCTCTTC	4 cut(s)	94, 136, 178, 488
SaqAI	TTAA	9 cut(s)	665, 891, 1518, 1685, 1760, 1872, 2397, 2799, 2999
Sau96I	GGNCC	2 cut(s)	564, 2986
SchI	GAGTC	1 cut(s)	3058
ScrFI	CCNGG	1 cut(s)	725
SduI	GDGCHC	3 cut(s)	508, 849, 2860
SfaNI	GCATC	7 cut(s)	769, 1135, 1571, 2470, 3112, 3292, 3293
SfcI	CTRYAG	3 cut(s)	1554, 2025, 2287
SinI	GGWCC	2 cut(s)	564, 2986
SmiMI	CAYNNNNRTG	1 cut(s)	2893
SmlI	CTYRAG	3 cut(s)	1246, 1382, 2600
SmoI	CTYRAG	3 cut(s)	1246, 1382, 2600
SspI	AATATT	2 cut(s)	1377, 1860
SspMI	CTAG	8 cut(s)	327, 1133, 1298, 1898, 1907, 2171, 2628, 3352
SstI	GAGCTC	1 cut(s)	849
StyD4I	CCNGG	1 cut(s)	723
TaaI	ACNGT	6 cut(s)	611, 986, 1003, 1046, 2357, 3204
TaiI	ACGT	8 cut(s)	324, 414, 686, 813, 1915, 2329, 3252, 3300
TaqI	TCGA	8 cut(s)	432, 765, 1143, 1792, 3040, 3054, 3246, 3300
TatI	WGTACW	2 cut(s)	1511, 2091
TauI	GCSGC	4 cut(s)	141, 237, 2065, 2738
TfiI	GAWTC	7 cut(s)	244, 578, 1166, 2209, 2668, 3104, 3162
Tru1I	TTAA	9 cut(s)	665, 891, 1518, 1685, 1760, 1872, 2397, 2799, 2999
Tru9I	TTAA	9 cut(s)	665, 891, 1518, 1685, 1760, 1872, 2397, 2799, 2999
TscAI	CASTG	9 cut(s)	236, 589, 616, 991, 1716, 2101, 2659, 2839, 3285
TseFI	GTSAC	4 cut(s)	1241, 1626, 2650, 2939
TseI	GCWGC	8 cut(s)	1810, 1927, 2289, 2389, 2468, 2713, 2738, 3305
Tsp45I	GTSAC	4 cut(s)	1241, 1626, 2650, 2939
TspGWI	ACGGA	3 cut(s)	1562, 1662, 1948
TspRI	CASTG	9 cut(s)	236, 589, 616, 991, 1716, 2101, 2659, 2839, 3285
Tth111I	GACNNNGTC	1 cut(s)	3251
VneI	GTGCAC	1 cut(s)	2856
VpaK11BI	GGWCC	2 cut(s)	564, 2986
XapI	RAATTY	7 cut(s)	25, 1028, 1173, 1769, 2586, 2699, 3230
XceI	RCATGY	1 cut(s)	2864
XcmI	CCANNNNNNNNNTGG	1 cut(s)	61
XmnI	GAANNNNTTC	2 cut(s)	2590, 3221
XspI	CTAG	8 cut(s)	327, 1133, 1298, 1898, 1907, 2171, 2628, 3352
ZraI	GACGTC	3 cut(s)	412, 811, 3298
Zsp2I	ATGCAT	1 cut(s)	697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.