Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0005171

Physical Location & Seq

Genomic Coordinates Reverse (-)

10111880 .. 10119400

Gene Structure 7521 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGATACGTCGGCAGCCGATGACAACTCCGGCGAACCCTTGACCCCAGTAGGTCGGCTCCTTCTCCAACCCGAAATGAACCAAATAATCCACTGTGCCATGGGATTCAAAAACTCCATAGACATAGACGCCGTAAAGTCTCACCTCAAAACCTCCCTCCTCCTCTCCCACCCAAGATTCTCAAGCCTCATGCTCCGCGACTCCCGCGGCCTCCAGCACTGGCACAATGCCCCCAACGTTGACCTCGACCGCCACATCATTGTCATCCACAACCCCGTCACCACATCTCCCGTCGATCACGAGACCGCAGTTAACGAATACTTGGCCGAACTCTCCACAAGCTCTACTAGACTGAGCGCGAACAAACCCCTCTGGGAACTTCACCTCCTCATGGCCCACAACTGCGGCATCTTCCGAATCCACCACGCTTTGGGAGACGGGGTTTCGGTCATGTCCCTATTCTTGGAGAGTTTCAGGACGAGAGGCTCAAATGGAAATGAAGAAAAGATTTTGGGTCGTGGAAGGAGGAAGAGAGTGAATGGTGAAAAGGGATGGTGGGGTTTGTTGATTCGGCGTGTGGAGATGTTGTGGTTTAGCTTGGTGTTTTTGGTGGAGAATGCGATGAGGAGCTTGTGGCTTTGTGACCAGAAAACAGCGATAAGTGGCGGCGATGGAGTTGAGCTCTGGCCTAGGAAGCTGGCCACGGCTAGGTTTCGGCTTGAGGACATGAAGCTCGTCAAGAAAGCTGTTCCGAATGCGACCATCAACGATGTTCTTGTTGGCGTGGTTTCGTCAGGGCTGTCAAGGTACCTAGATCACCAAACACCAAATGCTAAACTTCATTGTCCAGCTCTGCATGACGGAATTCGAATTACAGGCCTAGCTATGGTCAATTTAAGAGAGCAACCTGGATTGCAGGTATCCAATTTCTTGGAAAGCAACTCTGAATCAAGCTGGGGTAACAAGTTCGGCATGTTTCTCCTACCTATTTATTACCATAAAAGCAGTGATCTTGATCCTCTAGTGTACCTCAAGAGAGCTAAGCTGGTTCTTGACAGGAAGAAACAATCTCTAGAAGCTGATTTTTCGTACAAAGTTGGATGTTTTCTAATCACCTACTTTGGAGCAAAGGTTGCTAGTTGGCTTCTTTACAAGATGGTCTGCAATACCAGCTTTACCATTTCAAATATAATTGGCCCGCAAGAAGCAATTGCAATTGGGGGCAACCTTGTAACTTACCTTAGAGTGAATACGTGCAGCCTACCCCAGGCACTTACAATGCACATGGTGAGCTATGCTGAAAGAGCTGACCTGCAAATTTTGGTGGCCAAAGATATCATTCCGGATCCAACATTTCTCGCAAAATGCTTTGAAGAGGCATTGCTTGATATGAAGGAAGCAGCATCCAGCCAGCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009987 GO:0010025 GO:0010166 GO:0012505 GO:0016020 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0019432 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0047196 GO:0071704 GO:0071944 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1298 WES_acyltransf

Protein Analysis

473

Amino Acids

52.8

Weight (kDa)

8.51

Isoelectric Point (pI)

40.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WS_DGAT_cat	PF03007	72 - 258	3.8e-10	Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C	PF06974	319 - 465	3.5e-40	WS/DGAT C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005171)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g05740 FvH4_2g05740
malus_domestica	MD02G1104100.v1.1 MD05G1084200.v1.1 MD05G1084400.v1.1
prunus_persica	Prupe.8G128500_v2.0.a1 Prupe.8G128900_v2.0.a1 Prupe.8G129100_v2.0.a1
pyrus_communis	pycom05g08350 pycom10g07990
rosa_chinensis	RchiOBHm_Chr6g0256641
rosa_laevigata	RLG00000014747 RLG00000023074
rosa_multiflora	Rmu_co8379855.1_g000001 Rmu_sc0007004.1_g000003 Rmu_sc0039060.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395780 Rroxscaffold_7G00209140
rosa_rugosa	Rorug05G0565300 Rorug05G0565300
rosa_samantha	Rh3AG010700 Rh3BG010500 Rh3CG010200 Rh3DG010900 Rh5DG222300 Rh6AG083100 Rh6BG077700 Rh6CG072300 Rh6DG069500
rosa_wichuraiana	Rw6G007500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	907, 1320
Acc65I	GGTACC	1 cut(s)	807
AccB1I	GGYRCC	1 cut(s)	807
AccB7I	CCANNNNNTGG	1 cut(s)	430
AccII	CGCG	3 cut(s)	198, 207, 359
AccIII	TCCGGA	1 cut(s)	1342
AciI	CCGC	8 cut(s)	196, 205, 207, 250, 306, 405, 666, 1199
AclI	AACGTT	1 cut(s)	237
AclWI	GGATC	3 cut(s)	1010, 1340, 1353
AcoI	YGGCCR	3 cut(s)	324, 699, 1326
AcsI	RAATTY	2 cut(s)	864, 1317
AcyI	GRCGYC	1 cut(s)	129
AdeI	CACNNNGTG	1 cut(s)	1288
AfaI	GTAC	3 cut(s)	809, 1028, 1091
AfiI	CCNNNNNNNGG	6 cut(s)	391, 430, 463, 708, 886, 1267
AgsI	TTSAA	3 cut(s)	109, 1185, 1373
AjnI	CCWGG	2 cut(s)	907, 1266
AloI	GAACNNNNNNTCC	2 cut(s)	369, 401
Alw21I	GWGCWC	1 cut(s)	684
Alw26I	GTCTC	3 cut(s)	144, 296, 429
AlwI	GGATC	3 cut(s)	1010, 1340, 1353
Aor13HI	TCCGGA	1 cut(s)	1342
AoxI	GGCC	8 cut(s)	208, 324, 393, 686, 699, 877, 1195, 1326
ApeKI	GCWGC	3 cut(s)	14, 1257, 1400
ApoI	RAATTY	2 cut(s)	864, 1317
Asp718I	GGTACC	1 cut(s)	807
AspA2I	CCTAGG	1 cut(s)	689
AspLEI	GCGC	1 cut(s)	359
AspS9I	GGNCC	2 cut(s)	394, 1196
AsuHPI	GGTGA	7 cut(s)	134, 271, 374, 554, 809, 1105, 1300
AsuII	TTCGAA	1 cut(s)	868
AvrII	CCTAGG	1 cut(s)	689
BalI	TGGCCA	2 cut(s)	701, 1328
BamHI	GGATCC	1 cut(s)	1345
BanI	GGYRCC	1 cut(s)	807
BanII	GRGCYC	1 cut(s)	684
BauI	CACGAG	1 cut(s)	299
Bbv12I	GWGCWC	1 cut(s)	684
BbvI	GCAGC	3 cut(s)	26, 1269, 1412
BccI	CCATC	4 cut(s)	546, 665, 770, 1150
BceAI	ACGGC	2 cut(s)	116, 720
BciT130I	CCWGG	2 cut(s)	909, 1268
BciVI	GTATCC	1 cut(s)	931
BcoDI	GTCTC	3 cut(s)	144, 296, 429
BfaI	CTAG	8 cut(s)	348, 690, 708, 812, 881, 1022, 1073, 1137
BfuAI	ACCTGC	2 cut(s)	907, 1320
BfuI	GTATCC	1 cut(s)	931
BisI	GCNGC	6 cut(s)	15, 208, 406, 667, 1258, 1401
BlnI	CCTAGG	1 cut(s)	689
BlpI	GCTNAGC	1 cut(s)	1041
BlsI	GCNGC	6 cut(s)	16, 209, 407, 668, 1259, 1402
Bme1390I	CCNGG	2 cut(s)	909, 1268
BmgT120I	GGNCC	2 cut(s)	394, 1196
BmiI	GGNNCC	3 cut(s)	59, 809, 1347
BmrFI	CCNGG	2 cut(s)	909, 1268
BmrI	ACTGGG	1 cut(s)	41
BmsI	GCATC	2 cut(s)	417, 1412
BmuI	ACTGGG	1 cut(s)	41
BplI	GAGNNNNNCTC	2 cut(s)	666, 698
BpmI	CTGGAG	1 cut(s)	197
Bpu1102I	GCTNAGC	1 cut(s)	1041
Bpu14I	TTCGAA	1 cut(s)	868
BpuEI	CTTGAG	3 cut(s)	166, 740, 1016
BsaAI	YACGTR	1 cut(s)	1254
BsaBI	GATNNNNATC	1 cut(s)	1014
BsaHI	GRCGYC	1 cut(s)	129
BsaI	GGTCTC	1 cut(s)	296
BsaJI	CCNNGG	5 cut(s)	99, 205, 689, 702, 1266
BsaWI	WCCGGW	1 cut(s)	1342
Bsc4I	CCNNNNNNNGG	6 cut(s)	391, 430, 463, 708, 886, 1267
Bse1I	ACTGG	2 cut(s)	47, 224
Bse3DI	GCAATG	1 cut(s)	1379
Bse8I	GATNNNNATC	1 cut(s)	1014
BseAI	TCCGGA	1 cut(s)	1342
BseBI	CCWGG	2 cut(s)	909, 1268
BseDI	CCNNGG	5 cut(s)	99, 205, 689, 702, 1266
BseGI	GGATG	4 cut(s)	264, 557, 1106, 1403
BseJI	GATNNNNATC	1 cut(s)	1014
BseLI	CCNNNNNNNGG	6 cut(s)	391, 430, 463, 708, 886, 1267
BseMI	GCAATG	1 cut(s)	1379
BseMII	CTCAG	1 cut(s)	344
BseNI	ACTGG	2 cut(s)	47, 224
BseRI	GAGGAG	4 cut(s)	149, 152, 377, 640
BseXI	GCAGC	3 cut(s)	26, 1269, 1412
BseYI	CCCAGC	1 cut(s)	954
BsgI	GTGCAG	1 cut(s)	1276
Bsh1236I	CGCG	3 cut(s)	198, 207, 359
Bsh1285I	CGRYCG	1 cut(s)	250
BshFI	GGCC	8 cut(s)	210, 326, 395, 688, 701, 879, 1197, 1328
BshNI	GGYRCC	1 cut(s)	807
BsiEI	CGRYCG	1 cut(s)	250
BsiHKAI	GWGCWC	1 cut(s)	684
BsiSI	CCGG	2 cut(s)	30, 1343
BslFI	GGGAC	1 cut(s)	439
BslI	CCNNNNNNNGG	6 cut(s)	391, 430, 463, 708, 886, 1267
BsmAI	GTCTC	3 cut(s)	144, 296, 429
BsmBI	CGTCTC	1 cut(s)	429
BsmFI	GGGAC	1 cut(s)	439
BsmI	GAATGC	2 cut(s)	622, 760
BsnI	GGCC	8 cut(s)	210, 326, 395, 688, 701, 879, 1197, 1328
Bso31I	GGTCTC	1 cut(s)	296
Bsp119I	TTCGAA	1 cut(s)	868
Bsp1286I	GDGCHC	1 cut(s)	684
Bsp13I	TCCGGA	1 cut(s)	1342
Bsp143I	GATC	5 cut(s)	295, 814, 1009, 1015, 1345
Bsp1720I	GCTNAGC	1 cut(s)	1041
Bsp19I	CCATGG	1 cut(s)	99
BspACI	CCGC	8 cut(s)	196, 205, 207, 250, 306, 405, 666, 1199
BspANI	GGCC	8 cut(s)	210, 326, 395, 688, 701, 879, 1197, 1328
BspCNI	CTCAG	1 cut(s)	345
BspEI	TCCGGA	1 cut(s)	1342
BspFNI	CGCG	3 cut(s)	198, 207, 359
BspLI	GGNNCC	3 cut(s)	59, 809, 1347
BspMI	ACCTGC	2 cut(s)	907, 1320
BspPI	GGATC	3 cut(s)	1010, 1340, 1353
BspT104I	TTCGAA	1 cut(s)	868
BspT107I	GGYRCC	1 cut(s)	807
BspTNI	GGTCTC	1 cut(s)	296
BsrDI	GCAATG	1 cut(s)	1379
BsrI	ACTGG	2 cut(s)	47, 224
BssECI	CCNNGG	5 cut(s)	99, 205, 689, 702, 1266
BssMI	GATC	5 cut(s)	295, 814, 1009, 1015, 1345
BssNI	GRCGYC	1 cut(s)	129
BssSI	CACGAG	1 cut(s)	299
BssT1I	CCWWGG	2 cut(s)	99, 689
Bst2BI	CACGAG	1 cut(s)	299
Bst2UI	CCWGG	2 cut(s)	909, 1268
Bst4CI	ACNGT	1 cut(s)	95
Bst6I	CTCTTC	2 cut(s)	524, 1368
BstACI	GRCGYC	1 cut(s)	129
BstAPI	GCANNNNNTGC	1 cut(s)	1133
BstBAI	YACGTR	1 cut(s)	1254
BstBI	TTCGAA	1 cut(s)	868
BstC8I	GCNNGC	3 cut(s)	699, 1199, 1412
BstDEI	CTNAG	3 cut(s)	353, 1041, 1241
BstDSI	CCRYGG	3 cut(s)	99, 205, 702
BstENI	CCTNNNNNAGG	1 cut(s)	1265
BstF5I	GGATG	4 cut(s)	264, 557, 1106, 1403
BstFNI	CGCG	3 cut(s)	198, 207, 359
BstHHI	GCGC	1 cut(s)	359
BstKTI	GATC	5 cut(s)	298, 817, 1012, 1018, 1348
BstMAI	GTCTC	3 cut(s)	144, 296, 429
BstMBI	GATC	5 cut(s)	295, 814, 1009, 1015, 1345
BstMCI	CGRYCG	1 cut(s)	250
BstMWI	GCNNNNNNNGC	5 cut(s)	204, 694, 1133, 1170, 1304
BstNI	CCWGG	2 cut(s)	909, 1268
BstNSI	RCATGY	1 cut(s)	976
BstSCI	CCNGG	2 cut(s)	907, 1266
BstUI	CGCG	3 cut(s)	198, 207, 359
BstV1I	GCAGC	3 cut(s)	26, 1269, 1412
BstX2I	RGATCY	1 cut(s)	1345
BstXI	CCANNNNNNTGG	1 cut(s)	931
BstYI	RGATCY	1 cut(s)	1345
BsuI	GTATCC	1 cut(s)	931
BsuRI	GGCC	8 cut(s)	210, 326, 395, 688, 701, 879, 1197, 1328
BtgI	CCRYGG	3 cut(s)	99, 205, 702
BtgZI	GCGATG	2 cut(s)	635, 684
BtsCI	GGATG	4 cut(s)	264, 557, 1106, 1403
BtsI	GCAGTG	1 cut(s)	1012
BtsIMutI	CAGTG	3 cut(s)	91, 217, 1012
BveI	ACCTGC	2 cut(s)	907, 1320
Cac8I	GCNNGC	3 cut(s)	699, 1199, 1412
CfoI	GCGC	1 cut(s)	359
Cfr13I	GGNCC	2 cut(s)	394, 1196
Cfr42I	CCGCGG	1 cut(s)	208
CseI	GACGC	1 cut(s)	137
Csp6I	GTAC	3 cut(s)	808, 1027, 1090
CviAII	CATG	9 cut(s)	100, 190, 391, 451, 727, 857, 973, 1285, 1416
CviQI	GTAC	3 cut(s)	808, 1027, 1090
DdeI	CTNAG	3 cut(s)	353, 1041, 1241
DpnI	GATC	5 cut(s)	297, 816, 1011, 1017, 1347
DpnII	GATC	5 cut(s)	295, 814, 1009, 1015, 1345
DraIII	CACNNNGTG	1 cut(s)	1288
EaeI	YGGCCR	3 cut(s)	324, 699, 1326
Eam1104I	CTCTTC	2 cut(s)	524, 1368
EarI	CTCTTC	2 cut(s)	524, 1368
Ecl136II	GAGCTC	1 cut(s)	682
Eco130I	CCWWGG	2 cut(s)	99, 689
Eco147I	AGGCCT	1 cut(s)	879
Eco24I	GRGCYC	1 cut(s)	684
Eco31I	GGTCTC	1 cut(s)	296
Eco32I	GATATC	1 cut(s)	1336
Eco53kI	GAGCTC	1 cut(s)	682
EcoICRI	GAGCTC	1 cut(s)	682
EcoNI	CCTNNNNNAGG	1 cut(s)	1265
EcoRI	GAATTC	1 cut(s)	864
EcoRII	CCWGG	2 cut(s)	907, 1266
EcoRV	GATATC	1 cut(s)	1336
EcoT14I	CCWWGG	2 cut(s)	99, 689
EcoT38I	GRGCYC	1 cut(s)	684
ErhI	CCWWGG	2 cut(s)	99, 689
Esp3I	CGTCTC	1 cut(s)	429
FaeI	CATG	9 cut(s)	103, 193, 394, 454, 730, 860, 976, 1288, 1419
FaqI	GGGAC	1 cut(s)	439
FatI	CATG	9 cut(s)	99, 189, 390, 450, 726, 856, 972, 1284, 1415
FauI	CCCGC	2 cut(s)	212, 1206
Fnu4HI	GCNGC	6 cut(s)	15, 208, 406, 667, 1258, 1401
FokI	GGATG	4 cut(s)	251, 564, 1113, 1390
FriOI	GRGCYC	1 cut(s)	684
Fsp4HI	GCNGC	6 cut(s)	15, 208, 406, 667, 1258, 1401
FspBI	CTAG	8 cut(s)	348, 690, 708, 812, 881, 1022, 1073, 1137
GlaI	GCGC	1 cut(s)	358
GluI	GCNGC	6 cut(s)	15, 208, 406, 667, 1258, 1401
GsaI	CCCAGC	1 cut(s)	958
GsuI	CTGGAG	1 cut(s)	197
HaeIII	GGCC	8 cut(s)	210, 326, 395, 688, 701, 879, 1197, 1328
HapII	CCGG	2 cut(s)	30, 1343
HgaI	GACGC	1 cut(s)	137
HhaI	GCGC	1 cut(s)	359
Hin1I	GRCGYC	1 cut(s)	129
Hin1II	CATG	9 cut(s)	103, 193, 394, 454, 730, 860, 976, 1288, 1419
Hin6I	GCGC	1 cut(s)	357
HinP1I	GCGC	1 cut(s)	357
HincII	GTYRAC	2 cut(s)	241, 313
HindII	GTYRAC	2 cut(s)	241, 313
HinfI	GANTC	6 cut(s)	105, 177, 200, 417, 568, 947
HpaI	GTTAAC	1 cut(s)	313
HpaII	CCGG	2 cut(s)	30, 1343
HphI	GGTGA	7 cut(s)	134, 271, 374, 554, 809, 1105, 1300
Hpy166II	GTNNAC	3 cut(s)	241, 313, 1027
Hpy188I	TCNGA	3 cut(s)	416, 753, 946
Hpy188III	TCNNGA	8 cut(s)	299, 475, 739, 1013, 1033, 1052, 1073, 1343
Hpy8I	GTNNAC	3 cut(s)	241, 313, 1027
Hpy99I	CGWCG	2 cut(s)	13, 296
HpyAV	CCTTC	3 cut(s)	71, 516, 1387
HpyCH4III	ACNGT	1 cut(s)	95
HpyCH4IV	ACGT	3 cut(s)	8, 237, 1253
HpyCH4V	TGCA	7 cut(s)	856, 916, 1164, 1214, 1257, 1282, 1315
HpyF10VI	GCNNNNNNNGC	5 cut(s)	204, 694, 1133, 1170, 1304
HpyF3I	CTNAG	3 cut(s)	353, 1041, 1241
HpySE526I	ACGT	3 cut(s)	8, 237, 1253
Hsp92I	GRCGYC	1 cut(s)	129
Hsp92II	CATG	9 cut(s)	103, 193, 394, 454, 730, 860, 976, 1288, 1419
HspAI	GCGC	1 cut(s)	357
Kpn2I	TCCGGA	1 cut(s)	1342
KpnI	GGTACC	1 cut(s)	811
KspAI	GTTAAC	1 cut(s)	313
KspI	CCGCGG	1 cut(s)	208
Kzo9I	GATC	5 cut(s)	295, 814, 1009, 1015, 1345
LmnI	GCTCC	4 cut(s)	63, 198, 627, 1124
Lsp1109I	GCAGC	3 cut(s)	26, 1269, 1412
LweI	GCATC	2 cut(s)	417, 1412
MaeI	CTAG	8 cut(s)	348, 690, 708, 812, 881, 1022, 1073, 1137
MaeII	ACGT	3 cut(s)	8, 237, 1253
MaeIII	GTNAC	4 cut(s)	277, 641, 959, 1231
MalI	GATC	5 cut(s)	297, 816, 1011, 1017, 1347
MboI	GATC	5 cut(s)	295, 814, 1009, 1015, 1345
MboII	GAAGA	5 cut(s)	403, 512, 541, 1072, 1385
MfeI	CAATTG	2 cut(s)	1209, 1215
MflI	RGATCY	1 cut(s)	1345
MhlI	GDGCHC	1 cut(s)	684
MlsI	TGGCCA	2 cut(s)	701, 1328
MluCI	AATT	8 cut(s)	864, 870, 892, 925, 1191, 1209, 1215, 1317
MluNI	TGGCCA	2 cut(s)	701, 1328
MlyI	GAGTC	1 cut(s)	194
MmeI	TCCRAC	3 cut(s)	91, 1078, 1373
Mox20I	TGGCCA	2 cut(s)	701, 1328
MroI	TCCGGA	1 cut(s)	1342
MscI	TGGCCA	2 cut(s)	701, 1328
MseI	TTAA	2 cut(s)	312, 896
Msp20I	TGGCCA	2 cut(s)	701, 1328
MspA1I	CMGCKG	1 cut(s)	207
MspI	CCGG	2 cut(s)	30, 1343
MspR9I	CCNGG	2 cut(s)	909, 1268
MunI	CAATTG	2 cut(s)	1209, 1215
Mva1269I	GAATGC	2 cut(s)	622, 760
MvaI	CCWGG	2 cut(s)	909, 1268
MvnI	CGCG	3 cut(s)	198, 207, 359
MwoI	GCNNNNNNNGC	5 cut(s)	204, 694, 1133, 1170, 1304
NcoI	CCATGG	1 cut(s)	99
NdeII	GATC	5 cut(s)	295, 814, 1009, 1015, 1345
NlaIII	CATG	9 cut(s)	103, 193, 394, 454, 730, 860, 976, 1288, 1419
NlaIV	GGNNCC	3 cut(s)	59, 809, 1347
NmuCI	GTSAC	2 cut(s)	277, 641
NspI	RCATGY	1 cut(s)	976
NspV	TTCGAA	1 cut(s)	868
PceI	AGGCCT	1 cut(s)	879
PcsI	WCGNNNNNNNCGW	1 cut(s)	243
PctI	GAATGC	2 cut(s)	622, 760
PfeI	GAWTC	5 cut(s)	105, 177, 417, 568, 947
PflMI	CCANNNNNTGG	1 cut(s)	430
PkrI	GCNGC	6 cut(s)	16, 209, 407, 668, 1259, 1402
PleI	GAGTC	1 cut(s)	194
PpsI	GAGTC	1 cut(s)	194
Ppu21I	YACGTR	1 cut(s)	1254
Psp124BI	GAGCTC	1 cut(s)	684
Psp1406I	AACGTT	1 cut(s)	237
Psp6I	CCWGG	2 cut(s)	907, 1266
PspFI	CCCAGC	1 cut(s)	954
PspGI	CCWGG	2 cut(s)	907, 1266
PspN4I	GGNNCC	3 cut(s)	59, 809, 1347
PspPI	GGNCC	2 cut(s)	394, 1196
PsuI	RGATCY	1 cut(s)	1345
RsaI	GTAC	3 cut(s)	809, 1028, 1091
RsaNI	GTAC	3 cut(s)	808, 1027, 1090
SacI	GAGCTC	1 cut(s)	684
SacII	CCGCGG	1 cut(s)	208
SaqAI	TTAA	2 cut(s)	312, 896
SatI	GCNGC	6 cut(s)	15, 208, 406, 667, 1258, 1401
Sau3AI	GATC	5 cut(s)	295, 814, 1009, 1015, 1345
Sau96I	GGNCC	2 cut(s)	394, 1196
SchI	GAGTC	1 cut(s)	194
ScrFI	CCNGG	2 cut(s)	909, 1268
SduI	GDGCHC	1 cut(s)	684
SfaNI	GCATC	2 cut(s)	417, 1412
Sfr303I	CCGCGG	1 cut(s)	208
SfuI	TTCGAA	1 cut(s)	868
SgrBI	CCGCGG	1 cut(s)	208
SmlI	CTYRAG	3 cut(s)	181, 719, 1031
SmoI	CTYRAG	3 cut(s)	181, 719, 1031
Sse9I	AATT	8 cut(s)	864, 870, 892, 925, 1191, 1209, 1215, 1317
SseBI	AGGCCT	1 cut(s)	879
SsiI	CCGC	8 cut(s)	196, 205, 207, 250, 306, 405, 666, 1199
SspMI	CTAG	8 cut(s)	348, 690, 708, 812, 881, 1022, 1073, 1137
SstI	GAGCTC	1 cut(s)	684
StuI	AGGCCT	1 cut(s)	879
StyD4I	CCNGG	2 cut(s)	907, 1266
StyI	CCWWGG	2 cut(s)	99, 689
TaaI	ACNGT	1 cut(s)	95
TaiI	ACGT	3 cut(s)	11, 240, 1256
TaqI	TCGA	3 cut(s)	246, 294, 868
TaqII	GACCGA	1 cut(s)	436
TasI	AATT	8 cut(s)	864, 870, 892, 925, 1191, 1209, 1215, 1317
TauI	GCSGC	3 cut(s)	210, 408, 669
TfiI	GAWTC	5 cut(s)	105, 177, 417, 568, 947
Tru1I	TTAA	2 cut(s)	312, 896
Tru9I	TTAA	2 cut(s)	312, 896
TscAI	CASTG	3 cut(s)	98, 224, 1012
TseFI	GTSAC	2 cut(s)	277, 641
TseI	GCWGC	3 cut(s)	14, 1257, 1400
Tsp45I	GTSAC	2 cut(s)	277, 641
TspDTI	ATGAA	5 cut(s)	92, 513, 743, 830, 1406
TspGWI	ACGGA	1 cut(s)	876
TspRI	CASTG	3 cut(s)	98, 224, 1012
Van91I	CCANNNNNTGG	1 cut(s)	430
XagI	CCTNNNNNAGG	1 cut(s)	1265
XapI	RAATTY	2 cut(s)	864, 1317
XbaI	TCTAGA	1 cut(s)	1072
XceI	RCATGY	1 cut(s)	976
XmaJI	CCTAGG	1 cut(s)	689
XspI	CTAG	8 cut(s)	348, 690, 708, 812, 881, 1022, 1073, 1137

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.