pycom10g08630

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014644
Physical Location & Seq
Forward (+)
11379742 .. 11380638
897 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g08630.1

Sequence Viewer

Length: 765 bp
ATGGTAGAAAACCTCAGTTATGGGCCTAACCATGTTACGAAGGTCAGCGGTAATTGGGTCAAACAAAATCCTAGCCACTGCATTGAATCATTGATCAACATATATTCAAAATGCGGGAGTTTGGAGAGGGCAAAAGAAGTATTTTATCAGATGTTCATGAAAGATATTGTTTCATTCAACACCATGGTCATGGGTCTTGCGGTTAACAGTGAAGGAGATGAGGCTTTGAGGCTATTCTTTAAAAGCCAACAGTTCAGTTTGCAACCCAATGCAAGTATTTTCCTTGGTGCCTTATGTTATGACCTTAAGGTTTTCTGCTTATCCGAAATTAGAATGTATGCTTGTTACATTAATATCCTTGCAAAGGTAAGCCTACTTGAAGAAGCTTTAGAGGTTGTAAAATCCATGCCCCTTGAGCCTAATAATTTTGTGTGGGGTGCTCTGCTCGACGGCTGCCTGTTCCACTCTAGAGTACACGTGGCTCAACATGTTTCCAATAGACTGGCTCAAGTGGATCCTGAGAATTCTAGAGACTTTGTGATGCTAGCTAATACATTGGCATCCGATCATCGATGGGGTGATGTCTTTGAGTTGCGACGGTTTATGAGGGAAAAGAGGATGGCAATGCAACTCGGCTGTAGTTGGATCAGCATTAACGGTGTTGTGCAAGAATTTGTTGTGGGGTGTCCTTCACATCTTCAAATTGAGGGCATATTTAATACTTTAGACGGATTGGTGAAGCAAATGAAGGTAACAAGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

255

Amino Acids

28.8

Weight (kDa)

6.38

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 155 - 216 5.5e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014644)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25550
malus_domestica MD05G1093400.v1.1 MD10G1100300.v1.1
prunus_persica Prupe.8G138100_v2.0.a1
pyrus_communis pycom05g09250 pycom10g08630
rosa_chinensis RchiOBHm_Chr2g0123701
rosa_laevigata RLG00000018770
rosa_multiflora Rmu_sc0004915.1_g000001 Rmu_sc0006911.1_g000001
rosa_roxburghii Rroxscaffold_2G00120410
rosa_rugosa Rorug02G0247400
rosa_samantha Rh2AG306400 Rh2BG314800 Rh2CG292800 Rh2DG330100
rosa_wichuraiana Rw2G024580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 287
AciI CCGC 3 cut(s) 48, 114, 200
AclWI GGATC 3 cut(s) 509, 522, 653
AcsI RAATTY 2 cut(s) 523, 671
AcvI CACGTG 1 cut(s) 478
AfaI GTAC 1 cut(s) 474
AfiI CCNNNNNNNGG 1 cut(s) 364
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 2 cut(s) 475, 487
AgsI TTSAA 5 cut(s) 86, 108, 178, 380, 701
AluBI AGCT 2 cut(s) 386, 548
AluI AGCT 2 cut(s) 386, 548
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 1 cut(s) 525
AlwI GGATC 3 cut(s) 509, 522, 653
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 1 cut(s) 453
ApoI RAATTY 2 cut(s) 523, 671
AseI ATTAAT 1 cut(s) 351
AspS9I GGNCC 1 cut(s) 23
AsuHPI GGTGA 2 cut(s) 590, 748
AsuNHI GCTAGC 1 cut(s) 544
BamHI GGATCC 1 cut(s) 514
BanI GGYRCC 1 cut(s) 287
BbrPI CACGTG 1 cut(s) 478
Bbv12I GWGCWC 1 cut(s) 442
BbvI GCAGC 1 cut(s) 440
BccI CCATC 2 cut(s) 567, 613
BceAI ACGGC 1 cut(s) 466
BclI TGATCA 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 525
BfaI CTAG 4 cut(s) 72, 468, 528, 545
BfmI CTRYAG 1 cut(s) 637
BfrI CTTAAG 1 cut(s) 305
BisI GCNGC 1 cut(s) 454
BlsI GCNGC 1 cut(s) 455
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 2 cut(s) 289, 516
BmsI GCATC 2 cut(s) 531, 569
BmtI GCTAGC 1 cut(s) 548
BpuEI CTTGAG 2 cut(s) 434, 492
Bsa29I ATCGAT 1 cut(s) 571
BsaAI YACGTR 1 cut(s) 478
BsaJI CCNNGG 2 cut(s) 183, 283
Bsc4I CCNNNNNNNGG 1 cut(s) 364
Bse1I ACTGG 1 cut(s) 507
Bse3DI GCAATG 1 cut(s) 630
BseCI ATCGAT 1 cut(s) 571
BseDI CCNNGG 2 cut(s) 183, 283
BseGI GGATG 2 cut(s) 560, 624
BseLI CCNNNNNNNGG 1 cut(s) 364
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 2 cut(s) 28, 510
BseNI ACTGG 1 cut(s) 507
BseXI GCAGC 1 cut(s) 440
BshFI GGCC 1 cut(s) 25
BshNI GGYRCC 1 cut(s) 287
BshVI ATCGAT 1 cut(s) 571
BsiHKAI GWGCWC 1 cut(s) 442
BslI CCNNNNNNNGG 1 cut(s) 364
BsmAI GTCTC 1 cut(s) 525
BsnI GGCC 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 442
Bsp143I GATC 4 cut(s) 93, 514, 565, 645
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 3 cut(s) 48, 114, 200
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 2 cut(s) 27, 511
BspDI ATCGAT 1 cut(s) 571
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 289, 516
BspOI GCTAGC 1 cut(s) 548
BspPI GGATC 3 cut(s) 509, 522, 653
BspT107I GGYRCC 1 cut(s) 287
BspTI CTTAAG 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 630
BsrI ACTGG 1 cut(s) 507
BssECI CCNNGG 2 cut(s) 183, 283
BssMI GATC 4 cut(s) 93, 514, 565, 645
BssT1I CCWWGG 2 cut(s) 183, 283
Bst4CI ACNGT 4 cut(s) 209, 252, 600, 659
BstAFI CTTAAG 1 cut(s) 305
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 1 cut(s) 546
BstDEI CTNAG 2 cut(s) 14, 519
BstDSI CCRYGG 1 cut(s) 183
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 2 cut(s) 560, 624
BstKTI GATC 4 cut(s) 96, 517, 568, 648
BstMAI GTCTC 1 cut(s) 525
BstMBI GATC 4 cut(s) 93, 514, 565, 645
BstMWI GCNNNNNNNGC 1 cut(s) 415
BstNSI RCATGY 1 cut(s) 491
BstSFI CTRYAG 1 cut(s) 637
BstV1I GCAGC 1 cut(s) 440
BstX2I RGATCY 1 cut(s) 514
BstXI CCANNNNNNTGG 2 cut(s) 190, 502
BstYI RGATCY 1 cut(s) 514
Bsu15I ATCGAT 1 cut(s) 571
BsuRI GGCC 1 cut(s) 25
BsuTUI ATCGAT 1 cut(s) 571
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 2 cut(s) 560, 624
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 76, 214
Cac8I GCNNGC 1 cut(s) 546
CciI TCATGA 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 23
ClaI ATCGAT 1 cut(s) 571
Csp6I GTAC 1 cut(s) 473
CviAII CATG 6 cut(s) 32, 157, 184, 190, 406, 488
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 2 cut(s) 14, 519
DpnI GATC 4 cut(s) 95, 516, 567, 647
DpnII GATC 4 cut(s) 93, 514, 565, 645
DraI TTTAAA 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 183, 283
Eco72I CACGTG 1 cut(s) 478
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 523
EcoT14I CCWWGG 2 cut(s) 183, 283
ErhI CCWWGG 2 cut(s) 183, 283
FaeI CATG 6 cut(s) 35, 160, 187, 193, 409, 491
FatI CATG 6 cut(s) 31, 156, 183, 189, 405, 487
FauI CCCGC 1 cut(s) 107
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 1 cut(s) 454
FokI GGATG 2 cut(s) 547, 631
Fsp4HI GCNGC 1 cut(s) 454
FspBI CTAG 4 cut(s) 72, 468, 528, 545
GluI GCNGC 1 cut(s) 454
HaeIII GGCC 1 cut(s) 25
Hin1II CATG 6 cut(s) 35, 160, 187, 193, 409, 491
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 384
HinfI GANTC 1 cut(s) 86
HpaI GTTAAC 1 cut(s) 205
HphI GGTGA 2 cut(s) 590, 748
Hpy166II GTNNAC 2 cut(s) 205, 475
Hpy188I TCNGA 3 cut(s) 150, 325, 565
Hpy188III TCNNGA 4 cut(s) 157, 468, 518, 528
Hpy8I GTNNAC 2 cut(s) 205, 475
Hpy99I CGWCG 2 cut(s) 452, 600
HpyAV CCTTC 4 cut(s) 34, 206, 699, 742
HpyCH4III ACNGT 4 cut(s) 209, 252, 600, 659
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 6 cut(s) 81, 262, 272, 362, 628, 667
HpyF10VI GCNNNNNNNGC 1 cut(s) 415
HpyF3I CTNAG 2 cut(s) 14, 519
HpySE526I ACGT 1 cut(s) 477
Hsp92II CATG 6 cut(s) 35, 160, 187, 193, 409, 491
Ksp22I TGATCA 1 cut(s) 93
KspAI GTTAAC 1 cut(s) 205
Kzo9I GATC 4 cut(s) 93, 514, 565, 645
LpnPI CCDG 3 cut(s) 470, 488, 531
Lsp1109I GCAGC 1 cut(s) 440
LweI GCATC 2 cut(s) 531, 569
MaeI CTAG 4 cut(s) 72, 468, 528, 545
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 3 cut(s) 34, 344, 751
MalI GATC 4 cut(s) 95, 516, 567, 647
MboI GATC 4 cut(s) 93, 514, 565, 645
MboII GAAGA 2 cut(s) 392, 689
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 442
MluCI AATT 6 cut(s) 52, 327, 424, 523, 671, 702
MmeI TCCRAC 1 cut(s) 623
MnlI CCTC 8 cut(s) 23, 120, 214, 222, 385, 600, 609, 700
MseI TTAA 6 cut(s) 204, 240, 306, 351, 654, 717
MslI CAYNNNNRTG 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 48
MspCI CTTAAG 1 cut(s) 305
MwoI GCNNNNNNNGC 1 cut(s) 415
NcoI CCATGG 1 cut(s) 183
NdeII GATC 4 cut(s) 93, 514, 565, 645
NheI GCTAGC 1 cut(s) 544
NlaIII CATG 6 cut(s) 35, 160, 187, 193, 409, 491
NlaIV GGNNCC 2 cut(s) 289, 516
NmeAIII GCCGAG 1 cut(s) 612
NspI RCATGY 1 cut(s) 491
PagI TCATGA 1 cut(s) 156
PciI ACATGT 1 cut(s) 487
PfeI GAWTC 1 cut(s) 86
PkrI GCNGC 1 cut(s) 455
PmaCI CACGTG 1 cut(s) 478
PmlI CACGTG 1 cut(s) 478
Ppu21I YACGTR 1 cut(s) 478
PscI ACATGT 1 cut(s) 487
PshBI ATTAAT 1 cut(s) 351
PspCI CACGTG 1 cut(s) 478
PspN4I GGNNCC 2 cut(s) 289, 516
PspPI GGNCC 1 cut(s) 23
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
RseI CAYNNNNRTG 1 cut(s) 188
SaqAI TTAA 6 cut(s) 204, 240, 306, 351, 654, 717
SatI GCNGC 1 cut(s) 454
Sau3AI GATC 4 cut(s) 93, 514, 565, 645
Sau96I GGNCC 1 cut(s) 23
SduI GDGCHC 1 cut(s) 442
SfaNI GCATC 2 cut(s) 531, 569
SfcI CTRYAG 1 cut(s) 637
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 3 cut(s) 305, 413, 507
SmoI CTYRAG 3 cut(s) 305, 413, 507
Sse9I AATT 6 cut(s) 52, 327, 424, 523, 671, 702
SsiI CCGC 3 cut(s) 48, 114, 200
SspMI CTAG 4 cut(s) 72, 468, 528, 545
StyI CCWWGG 2 cut(s) 183, 283
TaaI ACNGT 4 cut(s) 209, 252, 600, 659
TaiI ACGT 1 cut(s) 480
TaqI TCGA 2 cut(s) 447, 571
TasI AATT 6 cut(s) 52, 327, 424, 523, 671, 702
TatI WGTACW 1 cut(s) 472
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 6 cut(s) 204, 240, 306, 351, 654, 717
Tru9I TTAA 6 cut(s) 204, 240, 306, 351, 654, 717
TscAI CASTG 2 cut(s) 83, 214
TseI GCWGC 1 cut(s) 453
TspDTI ATGAA 4 cut(s) 145, 162, 173, 761
TspGWI ACGGA 1 cut(s) 744
TspRI CASTG 2 cut(s) 83, 214
Vha464I CTTAAG 1 cut(s) 305
VspI ATTAAT 1 cut(s) 351
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 2 cut(s) 523, 671
XbaI TCTAGA 2 cut(s) 467, 527
XceI RCATGY 1 cut(s) 491
XspI CTAG 4 cut(s) 72, 468, 528, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.