Rh2BG314800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014644
Physical Location & Seq
Forward (+)
37073682 .. 37075100
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG314800.1

Sequence Viewer

Length: 1419 bp
ATGGGCTTTCTTCGCAACGAGTTCGTCTGTAATGGCCTGCTTGCTGTGTATGCTAAGGGACTCAAGGATCTGGGTTCTGCACGCCAGGTGTTTGATGAAATGCCTCACAAGGGTGTGGTTTGCTGTTGGACGTCTTTAATTGCTGGCTATGCTCAGTCAGGGAAGGCTGAGGAAGCTTTGCGGTTATTCGTGGTGATGGTTAAGGGAGATTTGCGGCCGGAGGATGATACAATGGTCAGTGTTTTATCCGCTTGTTCGAATCTTGACATGTCAAAGATTGAGAAATGGGTTTTGATTCTGTCCCGAGTTTTCGACAATGTGGATTCTAAGAATGTTGGTTCCGACTCCGTTAACACTGTACTGGTATATCTGTATGGGAAATGGGGGAAAATAGAGAAGAGCAGGGAAGCATTTGATGGAATTTCTGACAGTGGGAAGCCAAGTGTGCTGCCTTGGAATGCTATTATCGGTGCATACGTTCAGAATGGTTTTCCTGTGGAGTCTTTAAGTGTTTTCCGCGTAATGGTGGGAGATCCCACTCGTAAACCTAACCATGTTACTATGGTCAGTGTGCTTTCAGCCTGTGCTCAAGTTGGCGATTTAGAGCTTGGTAGATGGGTTCATGAATACTTGAAATCTAAAGGAAATAGAGGTGTGATTGGATCAAACAGAATTCTAGCCACTGCATTGATTGACATGTATTCCAAATGTGGGAGTTTGGAGAAGGCAAAAGAGGTTTTTCATCAGATGGTGTCGAAAGACATTGTTTCATTCAATGCTATGATCATGGGTCTTGCAGTTAACAGTGAAGGAGAGGAAGCCCTGAGGCTATTCTCAAAAATCCAAGATTTTGGTTTACAACCCAATGCTGGTACCTTCCTTGGTGCTTTATGTGCATGCAACCACTCAGGTTTTTCAGAGGAAGGGCATCAAATATTCAAGGATATGACCTCAGGCTTTTCCATTTCACCTAAATTAGAACATTATGCCTGCTATATTGATCTCCTTGCCCGAGTAGGCCTAGTTGAAGAAGCTCTCGAGGTTGTAACTTCCATGCCTTTTGAGCCTAACAGTATTGTGTGGGGTGCTCTGCTTGGAGGTTGCCTGCTCCACTCTAGATTAGACTATGCTGAATATGTTTCCCGTAAACTTGTTCAATCTGACCCCAACAATACCGGGGGTTATGTCATGCTGGCTAATGCATTTGCGTCTGATCATCGATGGGGTGATGTCTCTTCTCTGAGGCGGTTTATGAGGGAAAAGGGAGTGACCAAGCAGCCTGGCTGTAGTTGGATCAGCATTAATGGTGTTGTGCATGAATTTCTCGTTGGATGCTCGTCACATTCTCAAAGTGAAAGTATAAGTAGCATACTAAATGGATTGGTGAAACAAATGAAGATAGCAATTGACATACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

472

Amino Acids

51.68

Weight (kDa)

6.28

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 40 - 86 2.3e-07 PPR repeat family
PPR PF01535 42 - 68 3.8e-06 PPR repeat
PPR PF01535 228 - 255 9.6e-06 PPR repeat
PPR_2 PF13041 253 - 295 4.7e-07 PPR repeat family
E_motif PF20431 371 - 432 1.7e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014644)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25550
malus_domestica MD05G1093400.v1.1 MD10G1100300.v1.1
prunus_persica Prupe.8G138100_v2.0.a1
pyrus_communis pycom05g09250 pycom10g08630
rosa_chinensis RchiOBHm_Chr2g0123701
rosa_laevigata RLG00000018770
rosa_multiflora Rmu_sc0004915.1_g000001 Rmu_sc0006911.1_g000001
rosa_roxburghii Rroxscaffold_2G00120410
rosa_rugosa Rorug02G0247400
rosa_samantha Rh2AG306400 Rh2BG314800 Rh2CG292800 Rh2DG330100
rosa_wichuraiana Rw2G024580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 134
Acc65I GGTACC 1 cut(s) 872
AccB1I GGYRCC 1 cut(s) 872
AccII CGCG 1 cut(s) 519
AciI CCGC 5 cut(s) 181, 214, 249, 517, 1246
AclWI GGATC 4 cut(s) 75, 527, 670, 1301
AcoI YGGCCR 1 cut(s) 215
AcsI RAATTY 3 cut(s) 420, 672, 1319
AcyI GRCGYC 1 cut(s) 131
AfaI GTAC 2 cut(s) 360, 874
AfiI CCNNNNNNNGG 4 cut(s) 110, 523, 711, 869
AflIII ACRYGT 2 cut(s) 267, 696
AgsI TTSAA 5 cut(s) 634, 775, 940, 1028, 1157
AjnI CCWGG 2 cut(s) 84, 1279
AjuI GAANNNNNNNTTGG 2 cut(s) 1311, 1343
AleI CACNNNNGTG 1 cut(s) 111
AluBI AGCT 3 cut(s) 176, 607, 1034
AluI AGCT 3 cut(s) 176, 607, 1034
Alw21I GWGCWC 2 cut(s) 589, 1090
Alw26I GTCTC 1 cut(s) 1237
AlwI GGATC 4 cut(s) 75, 527, 670, 1301
Ama87I CYCGRG 3 cut(s) 303, 1011, 1037
AoxI GGCC 3 cut(s) 34, 215, 1018
ApeKI GCWGC 2 cut(s) 448, 1276
ApoI RAATTY 3 cut(s) 420, 672, 1319
AseI ATTAAT 1 cut(s) 1302
Asp718I GGTACC 1 cut(s) 872
AsuC2I CCSGG 1 cut(s) 1177
AsuHPI GGTGA 4 cut(s) 205, 960, 1238, 1396
AsuII TTCGAA 1 cut(s) 257
AvaI CYCGRG 3 cut(s) 303, 1011, 1037
AxyI CCTNAGG 2 cut(s) 824, 952
BaeI ACNNNNGTAYC 2 cut(s) 219, 252
BanI GGYRCC 1 cut(s) 872
Bbv12I GWGCWC 2 cut(s) 589, 1090
BbvCI CCTCAGC 1 cut(s) 168
BbvI GCAGC 2 cut(s) 435, 1288
BccI CCATC 5 cut(s) 190, 410, 609, 742, 1215
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 2 cut(s) 86, 1281
BclI TGATCA 2 cut(s) 783, 1213
BcnI CCSGG 1 cut(s) 1177
BcoDI GTCTC 1 cut(s) 1237
BfaI CTAG 3 cut(s) 677, 1022, 1116
BfmI CTRYAG 1 cut(s) 1285
BisI GCNGC 3 cut(s) 215, 449, 1277
BlsI GCNGC 3 cut(s) 216, 450, 1278
Bme1390I CCNGG 3 cut(s) 86, 1177, 1281
BmeT110I CYCGRG 3 cut(s) 303, 1011, 1037
BmiI GGNNCC 2 cut(s) 340, 874
BmrFI CCNGG 3 cut(s) 86, 1177, 1281
BmsI GCATC 2 cut(s) 937, 1322
Bpu10I CCTNAGC 2 cut(s) 54, 168
Bpu14I TTCGAA 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 47, 573
BpuMI CCSGG 1 cut(s) 1177
Bsa29I ATCGAT 1 cut(s) 1219
BsaHI GRCGYC 1 cut(s) 131
BsaJI CCNNGG 3 cut(s) 452, 880, 1176
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 523, 711, 869
Bse1I ACTGG 1 cut(s) 366
Bse21I CCTNAGG 2 cut(s) 824, 952
BseBI CCWGG 2 cut(s) 86, 1281
BseCI ATCGAT 1 cut(s) 1219
BseDI CCNNGG 3 cut(s) 452, 880, 1176
BseGI GGATG 2 cut(s) 229, 1337
BseLI CCNNNNNNNGG 4 cut(s) 110, 523, 711, 869
BseMII CTCAG 6 cut(s) 159, 167, 815, 921, 966, 1232
BseNI ACTGG 1 cut(s) 366
BseX3I CGGCCG 1 cut(s) 215
BseXI GCAGC 2 cut(s) 435, 1288
BsgI GTGCAG 1 cut(s) 63
Bsh1236I CGCG 1 cut(s) 519
Bsh1285I CGRYCG 1 cut(s) 218
BshFI GGCC 3 cut(s) 36, 217, 1020
BshNI GGYRCC 1 cut(s) 872
BshVI ATCGAT 1 cut(s) 1219
BsiEI CGRYCG 1 cut(s) 218
BsiHKAI GWGCWC 2 cut(s) 589, 1090
BsiHKCI CYCGRG 3 cut(s) 303, 1011, 1037
BsiSI CCGG 2 cut(s) 218, 1176
BslFI GGGAC 2 cut(s) 72, 286
BslI CCNNNNNNNGG 4 cut(s) 110, 523, 711, 869
BsmAI GTCTC 1 cut(s) 1237
BsmFI GGGAC 2 cut(s) 72, 286
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 3 cut(s) 36, 217, 1020
BsoBI CYCGRG 3 cut(s) 303, 1011, 1037
Bsp119I TTCGAA 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 589, 1090
Bsp143I GATC 7 cut(s) 67, 532, 662, 783, 1000, 1213, 1293
BspACI CCGC 5 cut(s) 181, 214, 249, 517, 1246
BspANI GGCC 3 cut(s) 36, 217, 1020
BspCNI CTCAG 6 cut(s) 160, 166, 816, 920, 965, 1233
BspDI ATCGAT 1 cut(s) 1219
BspFNI CGCG 1 cut(s) 519
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 2 cut(s) 340, 874
BspPI GGATC 4 cut(s) 75, 527, 670, 1301
BspQI GCTCTTC 1 cut(s) 392
BspT104I TTCGAA 1 cut(s) 257
BspT107I GGYRCC 1 cut(s) 872
BsrI ACTGG 1 cut(s) 366
BssECI CCNNGG 3 cut(s) 452, 880, 1176
BssMI GATC 7 cut(s) 67, 532, 662, 783, 1000, 1213, 1293
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 2 cut(s) 452, 880
Bst2UI CCWGG 2 cut(s) 86, 1281
Bst4CI ACNGT 4 cut(s) 358, 431, 806, 1073
Bst6I CTCTTC 2 cut(s) 392, 1240
BstACI GRCGYC 1 cut(s) 131
BstBI TTCGAA 1 cut(s) 257
BstC8I GCNNGC 8 cut(s) 38, 42, 82, 145, 898, 991, 1106, 1194
BstDEI CTNAG 8 cut(s) 54, 153, 168, 327, 824, 907, 952, 1241
BstF5I GGATG 2 cut(s) 229, 1337
BstFNI CGCG 1 cut(s) 519
BstKTI GATC 7 cut(s) 70, 535, 665, 786, 1003, 1216, 1296
BstMAI GTCTC 1 cut(s) 1237
BstMBI GATC 7 cut(s) 67, 532, 662, 783, 1000, 1213, 1293
BstMCI CGRYCG 1 cut(s) 218
BstMWI GCNNNNNNNGC 7 cut(s) 12, 50, 149, 173, 445, 893, 1063
BstNI CCWGG 2 cut(s) 86, 1281
BstNSI RCATGY 3 cut(s) 271, 700, 900
BstSCI CCNGG 3 cut(s) 84, 1175, 1279
BstSFI CTRYAG 1 cut(s) 1285
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 2 cut(s) 435, 1288
BstX2I RGATCY 2 cut(s) 67, 532
BstXI CCANNNNNNTGG 1 cut(s) 851
BstYI RGATCY 2 cut(s) 67, 532
BstZI CGGCCG 1 cut(s) 215
Bsu15I ATCGAT 1 cut(s) 1219
Bsu36I CCTNAGG 2 cut(s) 824, 952
BsuRI GGCC 3 cut(s) 36, 217, 1020
BsuTUI ATCGAT 1 cut(s) 1219
BtsCI GGATG 2 cut(s) 229, 1337
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 6 cut(s) 244, 354, 436, 574, 681, 811
Cac8I GCNNGC 8 cut(s) 38, 42, 82, 145, 898, 991, 1106, 1194
CciI TCATGA 1 cut(s) 622
ClaI ATCGAT 1 cut(s) 1219
CseI GACGC 1 cut(s) 1197
Csp6I GTAC 2 cut(s) 359, 873
CviAII CATG 9 cut(s) 268, 554, 623, 697, 787, 897, 1054, 1189, 1316
CviQI GTAC 2 cut(s) 359, 873
DdeI CTNAG 8 cut(s) 54, 153, 168, 327, 824, 907, 952, 1241
DpnI GATC 7 cut(s) 69, 534, 664, 785, 1002, 1215, 1295
DpnII GATC 7 cut(s) 67, 532, 662, 783, 1000, 1213, 1293
EaeI YGGCCR 1 cut(s) 215
EagI CGGCCG 1 cut(s) 215
Eam1104I CTCTTC 2 cut(s) 392, 1240
EarI CTCTTC 2 cut(s) 392, 1240
EclXI CGGCCG 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 452, 880
Eco147I AGGCCT 1 cut(s) 1020
Eco52I CGGCCG 1 cut(s) 215
Eco81I CCTNAGG 2 cut(s) 824, 952
Eco88I CYCGRG 3 cut(s) 303, 1011, 1037
EcoRI GAATTC 1 cut(s) 672
EcoRII CCWGG 2 cut(s) 84, 1279
EcoT14I CCWWGG 2 cut(s) 452, 880
EcoT22I ATGCAT 1 cut(s) 1204
ErhI CCWWGG 2 cut(s) 452, 880
FaeI CATG 9 cut(s) 271, 557, 626, 700, 790, 900, 1057, 1192, 1319
FaqI GGGAC 2 cut(s) 72, 286
FatI CATG 9 cut(s) 267, 553, 622, 696, 786, 896, 1053, 1188, 1315
FbaI TGATCA 2 cut(s) 783, 1213
Fnu4HI GCNGC 3 cut(s) 215, 449, 1277
FokI GGATG 2 cut(s) 236, 1344
Fsp4HI GCNGC 3 cut(s) 215, 449, 1277
FspBI CTAG 3 cut(s) 677, 1022, 1116
GluI GCNGC 3 cut(s) 215, 449, 1277
HaeIII GGCC 3 cut(s) 36, 217, 1020
HapII CCGG 2 cut(s) 218, 1176
HgaI GACGC 1 cut(s) 1197
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 9 cut(s) 271, 557, 626, 700, 790, 900, 1057, 1192, 1319
HincII GTYRAC 2 cut(s) 352, 802
HindII GTYRAC 2 cut(s) 352, 802
HindIII AAGCTT 1 cut(s) 174
HinfI GANTC 6 cut(s) 60, 259, 295, 323, 344, 500
HpaI GTTAAC 2 cut(s) 352, 802
HpaII CCGG 2 cut(s) 218, 1176
HphI GGTGA 4 cut(s) 205, 960, 1238, 1396
Hpy166II GTNNAC 5 cut(s) 352, 545, 802, 857, 1148
Hpy188I TCNGA 8 cut(s) 343, 427, 483, 747, 919, 1162, 1213, 1242
Hpy188III TCNNGA 5 cut(s) 263, 303, 623, 1037, 1116
Hpy8I GTNNAC 5 cut(s) 352, 545, 802, 857, 1148
HpyAV CCTTC 5 cut(s) 157, 718, 803, 886, 917
HpyCH4III ACNGT 4 cut(s) 358, 431, 806, 1073
HpyCH4IV ACGT 2 cut(s) 131, 477
HpyCH4V TGCA 8 cut(s) 80, 473, 686, 797, 896, 900, 1202, 1315
HpyF10VI GCNNNNNNNGC 7 cut(s) 12, 50, 149, 173, 445, 893, 1063
HpyF3I CTNAG 8 cut(s) 54, 153, 168, 327, 824, 907, 952, 1241
HpySE526I ACGT 2 cut(s) 131, 477
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 9 cut(s) 271, 557, 626, 700, 790, 900, 1057, 1192, 1319
KpnI GGTACC 1 cut(s) 876
Ksp22I TGATCA 2 cut(s) 783, 1213
KspAI GTTAAC 2 cut(s) 352, 802
Kzo9I GATC 7 cut(s) 67, 532, 662, 783, 1000, 1213, 1293
LguI GCTCTTC 1 cut(s) 392
LmnI GCTCC 1 cut(s) 1113
Lsp1109I GCAGC 2 cut(s) 435, 1288
LweI GCATC 2 cut(s) 937, 1322
MaeI CTAG 3 cut(s) 677, 1022, 1116
MaeII ACGT 2 cut(s) 131, 477
MaeIII GTNAC 4 cut(s) 556, 1045, 1267, 1338
MalI GATC 7 cut(s) 69, 534, 664, 785, 1002, 1215, 1295
MboI GATC 7 cut(s) 67, 532, 662, 783, 1000, 1213, 1293
MboII GAAGA 4 cut(s) 409, 1040, 1227, 1408
MfeI CAATTG 1 cut(s) 1404
MflI RGATCY 2 cut(s) 67, 532
MhlI GDGCHC 2 cut(s) 589, 1090
MluCI AATT 6 cut(s) 138, 420, 672, 974, 1319, 1404
MlyI GAGTC 3 cut(s) 54, 338, 509
MmeI TCCRAC 4 cut(s) 107, 366, 1271, 1309
Mph1103I ATGCAT 1 cut(s) 1204
MseI TTAA 6 cut(s) 137, 201, 351, 506, 801, 1302
MslI CAYNNNNRTG 1 cut(s) 111
MspI CCGG 2 cut(s) 218, 1176
MspR9I CCNGG 3 cut(s) 86, 1177, 1281
MunI CAATTG 1 cut(s) 1404
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 2 cut(s) 86, 1281
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 7 cut(s) 12, 50, 149, 173, 445, 893, 1063
NciI CCSGG 1 cut(s) 1177
NdeII GATC 7 cut(s) 67, 532, 662, 783, 1000, 1213, 1293
NlaIII CATG 9 cut(s) 271, 557, 626, 700, 790, 900, 1057, 1192, 1319
NlaIV GGNNCC 2 cut(s) 340, 874
NmuCI GTSAC 2 cut(s) 1267, 1338
NsiI ATGCAT 1 cut(s) 1204
NspI RCATGY 3 cut(s) 271, 700, 900
NspV TTCGAA 1 cut(s) 257
OliI CACNNNNGTG 1 cut(s) 111
PaeI GCATGC 1 cut(s) 900
PaeR7I CTCGAG 1 cut(s) 1037
PagI TCATGA 1 cut(s) 622
PceI AGGCCT 1 cut(s) 1020
PciI ACATGT 2 cut(s) 267, 696
PciSI GCTCTTC 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PctI GAATGC 1 cut(s) 463
PfeI GAWTC 3 cut(s) 259, 295, 323
PkrI GCNGC 3 cut(s) 216, 450, 1278
PleI GAGTC 3 cut(s) 54, 338, 508
PpsI GAGTC 3 cut(s) 54, 338, 508
PscI ACATGT 2 cut(s) 267, 696
PshBI ATTAAT 1 cut(s) 1302
Psp6I CCWGG 2 cut(s) 84, 1279
PspGI CCWGG 2 cut(s) 84, 1279
PspN4I GGNNCC 2 cut(s) 340, 874
PsuI RGATCY 2 cut(s) 67, 532
RsaI GTAC 2 cut(s) 360, 874
RsaNI GTAC 2 cut(s) 359, 873
RseI CAYNNNNRTG 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 392
SaqAI TTAA 6 cut(s) 137, 201, 351, 506, 801, 1302
SatI GCNGC 3 cut(s) 215, 449, 1277
Sau3AI GATC 7 cut(s) 67, 532, 662, 783, 1000, 1213, 1293
SchI GAGTC 3 cut(s) 54, 338, 509
ScrFI CCNGG 3 cut(s) 86, 1177, 1281
SduI GDGCHC 2 cut(s) 589, 1090
SfaNI GCATC 2 cut(s) 937, 1322
SfcI CTRYAG 1 cut(s) 1285
Sfr274I CTCGAG 1 cut(s) 1037
SfuI TTCGAA 1 cut(s) 257
SlaI CTCGAG 1 cut(s) 1037
SmiMI CAYNNNNRTG 1 cut(s) 111
SmlI CTYRAG 3 cut(s) 62, 588, 1037
SmoI CTYRAG 3 cut(s) 62, 588, 1037
SphI GCATGC 1 cut(s) 900
Sse9I AATT 6 cut(s) 138, 420, 672, 974, 1319, 1404
SseBI AGGCCT 1 cut(s) 1020
SsiI CCGC 5 cut(s) 181, 214, 249, 517, 1246
SspI AATATT 1 cut(s) 936
SspMI CTAG 3 cut(s) 677, 1022, 1116
StuI AGGCCT 1 cut(s) 1020
StyD4I CCNGG 3 cut(s) 84, 1175, 1279
StyI CCWWGG 2 cut(s) 452, 880
TaaI ACNGT 4 cut(s) 358, 431, 806, 1073
TaiI ACGT 2 cut(s) 134, 480
TaqI TCGA 5 cut(s) 257, 312, 755, 1038, 1219
TasI AATT 6 cut(s) 138, 420, 672, 974, 1319, 1404
TatI WGTACW 1 cut(s) 358
TauI GCSGC 1 cut(s) 217
TfiI GAWTC 3 cut(s) 259, 295, 323
Tru1I TTAA 6 cut(s) 137, 201, 351, 506, 801, 1302
Tru9I TTAA 6 cut(s) 137, 201, 351, 506, 801, 1302
TscAI CASTG 6 cut(s) 244, 361, 436, 574, 688, 811
TseFI GTSAC 2 cut(s) 1267, 1338
TseI GCWGC 2 cut(s) 448, 1276
Tsp45I GTSAC 2 cut(s) 1267, 1338
TspDTI ATGAA 7 cut(s) 111, 611, 639, 731, 759, 1332, 1409
TspGWI ACGGA 1 cut(s) 337
TspRI CASTG 6 cut(s) 244, 361, 436, 574, 688, 811
VspI ATTAAT 1 cut(s) 1302
XapI RAATTY 3 cut(s) 420, 672, 1319
XbaI TCTAGA 1 cut(s) 1115
XceI RCATGY 3 cut(s) 271, 700, 900
XhoI CTCGAG 1 cut(s) 1037
XspI CTAG 3 cut(s) 677, 1022, 1116
ZraI GACGTC 1 cut(s) 132
Zsp2I ATGCAT 1 cut(s) 1204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.