Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0014644

Physical Location & Seq

Genomic Coordinates Forward (+)

35716119 .. 35717975

Gene Structure 1857 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018770

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGAACTTGCTTCTCTCATCTCAAAAGCTCAAGACTTGCTATTCGTCTCACTCTCTTCTTCACATCTCTGCCGACTTAGCTGCTCTGCTACAAGGTCGTATCTCACACTCTCATCTCCTTCAAATCCATGCCCAAATTTTTGTTCTTGGTGCTCACCACGACGATTTGATAGCCACTCGTCTCATTGGCCACTACCCATCTCACATTGCTCTCCGTGTCTTTCATGGGCTCCGAAAACCCAATGTGTTCCCTTTCAACGCCATCATCAGGGTCTTCGCCGAACAGGGTCTCTTCTGCAATGCCTTCTCCCTCTTTAAAACCCTGAAACAGAGGTCCCTTTCGCCCAATGACTTCACCTTCTCTTTCCTTCTCAAGTCGTGCAGCTTTCGTTGCGGGGATTCTGTTTATGTCAAGCAAATTCACACCCATGTTGTGAAGATGGGGTTTCTTCGCAACGAGTTCGTCTGTAATGGCCTGCTTGCTGTGTATGGTAAGGGACTCAAGGATTTGGGTTCTGCACGCCAGGTGTTTGATGAAATGCCTGACAAGGGTGTGGTTTGCTGTTGGACGTCTTTAATTGCGGGTTATGCTCAGTCAGGGAAGGCTGAGGAAGCTTTGCGGTTATTCGTGATGATGGTTAAGGGAAATTTGCGGCCGGAGGATGATACAATGGTCAGTGTTTTATCAGCTTGTTCGAATCTTGACATGTCAAAGATTGAGAAATGGGTTTTGATTCTGTCCCGAGTTTTCGACAATGTGGATTCTAAGAATGTTGGTTTGGACTCCGTTAACACTGTACTGATATATCTGTATGGGAAATGGGGGAAAATAGAGAAGAGCAGGGAAGCATTTGATGGAATTTCTGACAGTGGGAAGCGAAGTGTGCTGCCTTGGAATGCTATTATCGGTGCATATGTTCAGAATGGTTTTCCTGTGGAGTCTTTAAGTGTTTTCCGTGTAATGGTGGGAGATCCCACTCGTAAACCTAACCATGTTACTATGGTCAGTGTGCTTTCAGCTTGTGCTCAAGTTGGCGATTTAGAGCTTGGTAGGTGGGTTCATGAATACTTGAAATCTAAAGGAAATAGAGGTGTGATTGGATCAAACAGAATTCTAGCCACTGCATTGATTGACATGTATTCCAAATGTGGGAGTTTGGAGAAGGCAAAAGAGATTTTTCATCAGATGGTGTCGAAAGACATTGTTTCATTCAATGCTATGATCATGGGTCTTGCAGTTAACAGTGAAGGAGAGGAAGCCCTCGGGCTATTCTCAAAAATCCAAGATTTTGGTTTACAACCCAATGCTGGTACCTTCCTTGGTGCTTTATGTGCATGCAACCACTCAGGTTTTTCAGAGGAAGGGCGTCAAATATTCAAGGATATGACCTCAGGCTTTTCCATTGCACCTAAATTAGAACATTATGCCTGCTATATTGATCTCCTTGCCCGAGTAGGCCTAGTTGAAGAAGCTCTCGAGGTTGTAACTTCCATGCCTTTTGAGCCTAACAGTATTGTGTGGGGTGCTCTGCTTGGAGGTTGCCTGCTCCACTCTAGATTAGACAATGCTGAATATGTTTCCCGTAAACTTGTTCAATCTGACCCCAACAATACCGGGGGTTATGTCATGCTGGCTAACGCATTTGCGTCTGATTATCGATGGGGTGATGTCTCTTCTCTGAGGCGGTTTATGAGGGAAAAAGGAGTGACCAAGCAGCCTGGCTGTAGTTGGATCAGCATTAATGGTGTTGTGCATGAATTTCTTGTTGGATGCTCGTCACATTCTAAAAGTGAAAGTATAAGTAGCATACTAAATGGATTGGTGAAACAAATGAAGATAGCAATTGACATACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

619

Amino Acids

68.16

Weight (kDa)

8.56

Isoelectric Point (pI)

39.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	80 - 127	1.8e-09	PPR repeat family
PPR_2	PF13041	186 - 232	5.2e-08	PPR repeat family
PPR	PF01535	188 - 214	1.1e-06	PPR repeat
PPR_2	PF13041	399 - 441	3.2e-06	PPR repeat family
E_motif	PF20431	517 - 578	6.4e-15	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014644)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g25550
malus_domestica	MD05G1093400.v1.1 MD10G1100300.v1.1
prunus_persica	Prupe.8G138100_v2.0.a1
pyrus_communis	pycom05g09250 pycom10g08630
rosa_chinensis	RchiOBHm_Chr2g0123701
rosa_laevigata	RLG00000018770
rosa_multiflora	Rmu_sc0004915.1_g000001 Rmu_sc0006911.1_g000001
rosa_roxburghii	Rroxscaffold_2G00120410
rosa_rugosa	Rorug02G0247400
rosa_samantha	Rh2AG306400 Rh2BG314800 Rh2CG292800 Rh2DG330100
rosa_wichuraiana	Rw2G024580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	572
Acc65I	GGTACC	1 cut(s)	1310
AccB1I	GGYRCC	1 cut(s)	1310
AciI	CCGC	5 cut(s)	393, 581, 619, 652, 1684
AclWI	GGATC	3 cut(s)	965, 1108, 1739
AcoI	YGGCCR	2 cut(s)	187, 653
AcsI	RAATTY	6 cut(s)	135, 417, 646, 858, 1110, 1757
AcyI	GRCGYC	2 cut(s)	569, 1366
AfaI	GTAC	2 cut(s)	798, 1312
AfiI	CCNNNNNNNGG	5 cut(s)	267, 548, 961, 1149, 1307
AflIII	ACRYGT	2 cut(s)	705, 1134
AgsI	TTSAA	7 cut(s)	122, 256, 1072, 1213, 1378, 1466, 1595
AjnI	CCWGG	2 cut(s)	522, 1717
AjuI	GAANNNNNNNTTGG	6 cut(s)	126, 158, 761, 793, 1749, 1781
AluBI	AGCT	8 cut(s)	28, 80, 384, 614, 689, 1019, 1045, 1472
AluI	AGCT	8 cut(s)	28, 80, 384, 614, 689, 1019, 1045, 1472
Alw21I	GWGCWC	3 cut(s)	154, 1027, 1528
Alw26I	GTCTC	4 cut(s)	51, 185, 293, 1675
AlwI	GGATC	3 cut(s)	965, 1108, 1739
Ama87I	CYCGRG	4 cut(s)	741, 1262, 1449, 1475
AoxI	GGCC	4 cut(s)	187, 472, 653, 1456
ApeKI	GCWGC	4 cut(s)	80, 381, 886, 1714
ApoI	RAATTY	6 cut(s)	135, 417, 646, 858, 1110, 1757
ArsI	GACNNNNNNTTYG	2 cut(s)	25, 57
AseI	ATTAAT	1 cut(s)	1740
Asp718I	GGTACC	1 cut(s)	1310
AspS9I	GGNCC	1 cut(s)	333
AsuC2I	CCSGG	1 cut(s)	1615
AsuHPI	GGTGA	4 cut(s)	146, 346, 1676, 1834
AsuII	TTCGAA	1 cut(s)	695
AvaI	CYCGRG	4 cut(s)	741, 1262, 1449, 1475
AvaII	GGWCC	1 cut(s)	333
AxyI	CCTNAGG	1 cut(s)	1390
BaeI	ACNNNNGTAYC	2 cut(s)	657, 690
BalI	TGGCCA	1 cut(s)	189
BanI	GGYRCC	1 cut(s)	1310
BanII	GRGCYC	1 cut(s)	231
BbsI	GAAGAC	1 cut(s)	265
Bbv12I	GWGCWC	3 cut(s)	154, 1027, 1528
BbvCI	CCTCAGC	1 cut(s)	606
BbvI	GCAGC	4 cut(s)	67, 393, 873, 1726
BccI	CCATC	7 cut(s)	205, 269, 433, 628, 848, 1180, 1653
BcgI	CGANNNNNNTGC	4 cut(s)	62, 96, 442, 476
BciT130I	CCWGG	2 cut(s)	524, 1719
BclI	TGATCA	1 cut(s)	1221
BcnI	CCSGG	1 cut(s)	1615
BcoDI	GTCTC	4 cut(s)	51, 185, 293, 1675
BfaI	CTAG	3 cut(s)	1115, 1460, 1554
BfmI	CTRYAG	1 cut(s)	1723
BisI	GCNGC	5 cut(s)	81, 382, 653, 887, 1715
BlsI	GCNGC	5 cut(s)	82, 383, 654, 888, 1716
Bme1390I	CCNGG	3 cut(s)	524, 1615, 1719
Bme18I	GGWCC	1 cut(s)	333
BmeT110I	CYCGRG	4 cut(s)	741, 1262, 1449, 1475
BmgT120I	GGNCC	1 cut(s)	333
BmiI	GGNNCC	3 cut(s)	230, 335, 1312
BmrFI	CCNGG	3 cut(s)	524, 1615, 1719
BmsI	GCATC	1 cut(s)	1760
BpiI	GAAGAC	1 cut(s)	265
Bpu10I	CCTNAGC	1 cut(s)	606
Bpu14I	TTCGAA	1 cut(s)	695
BpuEI	CTTGAG	4 cut(s)	14, 356, 485, 1011
BpuMI	CCSGG	1 cut(s)	1615
Bsa29I	ATCGAT	1 cut(s)	1657
BsaHI	GRCGYC	2 cut(s)	569, 1366
BsaI	GGTCTC	1 cut(s)	293
BsaJI	CCNNGG	4 cut(s)	890, 1261, 1318, 1614
Bsc4I	CCNNNNNNNGG	5 cut(s)	267, 548, 961, 1149, 1307
Bse21I	CCTNAGG	1 cut(s)	1390
Bse3DI	GCAATG	3 cut(s)	204, 304, 1401
BseBI	CCWGG	2 cut(s)	524, 1719
BseCI	ATCGAT	1 cut(s)	1657
BseDI	CCNNGG	4 cut(s)	890, 1261, 1318, 1614
BseGI	GGATG	2 cut(s)	667, 1775
BseLI	CCNNNNNNNGG	5 cut(s)	267, 548, 961, 1149, 1307
BseMI	GCAATG	3 cut(s)	204, 304, 1401
BseMII	CTCAG	5 cut(s)	597, 605, 1359, 1404, 1670
BseX3I	CGGCCG	1 cut(s)	653
BseXI	GCAGC	4 cut(s)	67, 393, 873, 1726
BsgI	GTGCAG	2 cut(s)	400, 501
Bsh1285I	CGRYCG	1 cut(s)	656
BshFI	GGCC	4 cut(s)	189, 474, 655, 1458
BshNI	GGYRCC	1 cut(s)	1310
BshVI	ATCGAT	1 cut(s)	1657
BsiEI	CGRYCG	1 cut(s)	656
BsiHKAI	GWGCWC	3 cut(s)	154, 1027, 1528
BsiHKCI	CYCGRG	4 cut(s)	741, 1262, 1449, 1475
BsiSI	CCGG	2 cut(s)	656, 1614
BslFI	GGGAC	3 cut(s)	319, 510, 724
BslI	CCNNNNNNNGG	5 cut(s)	267, 548, 961, 1149, 1307
BsmAI	GTCTC	4 cut(s)	51, 185, 293, 1675
BsmBI	CGTCTC	2 cut(s)	51, 185
BsmFI	GGGAC	3 cut(s)	319, 510, 724
BsmI	GAATGC	1 cut(s)	901
BsnI	GGCC	4 cut(s)	189, 474, 655, 1458
Bso31I	GGTCTC	1 cut(s)	293
BsoBI	CYCGRG	4 cut(s)	741, 1262, 1449, 1475
Bsp119I	TTCGAA	1 cut(s)	695
Bsp1286I	GDGCHC	4 cut(s)	154, 231, 1027, 1528
Bsp143I	GATC	5 cut(s)	970, 1100, 1221, 1438, 1731
BspACI	CCGC	5 cut(s)	393, 581, 619, 652, 1684
BspANI	GGCC	4 cut(s)	189, 474, 655, 1458
BspCNI	CTCAG	5 cut(s)	598, 604, 1358, 1403, 1671
BspDI	ATCGAT	1 cut(s)	1657
BspHI	TCATGA	1 cut(s)	1060
BspLI	GGNNCC	3 cut(s)	230, 335, 1312
BspPI	GGATC	3 cut(s)	965, 1108, 1739
BspQI	GCTCTTC	1 cut(s)	830
BspT104I	TTCGAA	1 cut(s)	695
BspT107I	GGYRCC	1 cut(s)	1310
BspTNI	GGTCTC	1 cut(s)	293
BsrDI	GCAATG	3 cut(s)	204, 304, 1401
BssECI	CCNNGG	4 cut(s)	890, 1261, 1318, 1614
BssMI	GATC	5 cut(s)	970, 1100, 1221, 1438, 1731
BssNI	GRCGYC	2 cut(s)	569, 1366
BssT1I	CCWWGG	2 cut(s)	890, 1318
Bst2UI	CCWGG	2 cut(s)	524, 1719
Bst4CI	ACNGT	4 cut(s)	796, 869, 1244, 1511
Bst6I	CTCTTC	4 cut(s)	60, 296, 830, 1678
BstACI	GRCGYC	2 cut(s)	569, 1366
BstBI	TTCGAA	1 cut(s)	695
BstC8I	GCNNGC	7 cut(s)	476, 480, 520, 1336, 1429, 1544, 1632
BstDEI	CTNAG	7 cut(s)	76, 591, 606, 765, 1345, 1390, 1679
BstF5I	GGATG	2 cut(s)	667, 1775
BstKTI	GATC	5 cut(s)	973, 1103, 1224, 1441, 1734
BstMAI	GTCTC	4 cut(s)	51, 185, 293, 1675
BstMBI	GATC	5 cut(s)	970, 1100, 1221, 1438, 1731
BstMCI	CGRYCG	1 cut(s)	656
BstMWI	GCNNNNNNNGC	7 cut(s)	77, 390, 587, 611, 883, 1331, 1501
BstNI	CCWGG	2 cut(s)	524, 1719
BstNSI	RCATGY	3 cut(s)	709, 1138, 1338
BstSCI	CCNGG	3 cut(s)	522, 1613, 1717
BstSFI	CTRYAG	1 cut(s)	1723
BstV1I	GCAGC	4 cut(s)	67, 393, 873, 1726
BstV2I	GAAGAC	1 cut(s)	265
BstX2I	RGATCY	1 cut(s)	970
BstXI	CCANNNNNNTGG	1 cut(s)	1289
BstYI	RGATCY	1 cut(s)	970
BstZI	CGGCCG	1 cut(s)	653
Bsu15I	ATCGAT	1 cut(s)	1657
Bsu36I	CCTNAGG	1 cut(s)	1390
BsuRI	GGCC	4 cut(s)	189, 474, 655, 1458
BsuTUI	ATCGAT	1 cut(s)	1657
BtsCI	GGATG	2 cut(s)	667, 1775
BtsI	GCAGTG	1 cut(s)	1119
BtsIMutI	CAGTG	6 cut(s)	682, 792, 874, 1012, 1119, 1249
Cac8I	GCNNGC	7 cut(s)	476, 480, 520, 1336, 1429, 1544, 1632
CciI	TCATGA	1 cut(s)	1060
Cfr13I	GGNCC	1 cut(s)	333
ClaI	ATCGAT	1 cut(s)	1657
CseI	GACGC	2 cut(s)	1355, 1635
Csp6I	GTAC	2 cut(s)	797, 1311
CviQI	GTAC	2 cut(s)	797, 1311
DdeI	CTNAG	7 cut(s)	76, 591, 606, 765, 1345, 1390, 1679
DpnI	GATC	5 cut(s)	972, 1102, 1223, 1440, 1733
DpnII	GATC	5 cut(s)	970, 1100, 1221, 1438, 1731
DraI	TTTAAA	1 cut(s)	316
EaeI	YGGCCR	2 cut(s)	187, 653
EagI	CGGCCG	1 cut(s)	653
Eam1104I	CTCTTC	4 cut(s)	60, 296, 830, 1678
EarI	CTCTTC	4 cut(s)	60, 296, 830, 1678
EclXI	CGGCCG	1 cut(s)	653
Eco130I	CCWWGG	2 cut(s)	890, 1318
Eco147I	AGGCCT	1 cut(s)	1458
Eco24I	GRGCYC	1 cut(s)	231
Eco31I	GGTCTC	1 cut(s)	293
Eco47I	GGWCC	1 cut(s)	333
Eco52I	CGGCCG	1 cut(s)	653
Eco81I	CCTNAGG	1 cut(s)	1390
Eco88I	CYCGRG	4 cut(s)	741, 1262, 1449, 1475
EcoO109I	RGGNCCY	1 cut(s)	333
EcoRI	GAATTC	1 cut(s)	1110
EcoRII	CCWGG	2 cut(s)	522, 1717
EcoT14I	CCWWGG	2 cut(s)	890, 1318
EcoT38I	GRGCYC	1 cut(s)	231
ErhI	CCWWGG	2 cut(s)	890, 1318
Esp3I	CGTCTC	2 cut(s)	51, 185
FaqI	GGGAC	3 cut(s)	319, 510, 724
FauI	CCCGC	2 cut(s)	386, 574
FauNDI	CATATG	1 cut(s)	913
FbaI	TGATCA	1 cut(s)	1221
Fnu4HI	GCNGC	5 cut(s)	81, 382, 653, 887, 1715
FokI	GGATG	2 cut(s)	674, 1782
FriOI	GRGCYC	1 cut(s)	231
Fsp4HI	GCNGC	5 cut(s)	81, 382, 653, 887, 1715
FspBI	CTAG	3 cut(s)	1115, 1460, 1554
GluI	GCNGC	5 cut(s)	81, 382, 653, 887, 1715
HaeIII	GGCC	4 cut(s)	189, 474, 655, 1458
HapII	CCGG	2 cut(s)	656, 1614
HgaI	GACGC	2 cut(s)	1355, 1635
Hin1I	GRCGYC	2 cut(s)	569, 1366
HincII	GTYRAC	2 cut(s)	790, 1240
HindII	GTYRAC	2 cut(s)	790, 1240
HindIII	AAGCTT	1 cut(s)	612
HinfI	GANTC	7 cut(s)	398, 498, 697, 733, 761, 782, 938
HpaI	GTTAAC	2 cut(s)	790, 1240
HpaII	CCGG	2 cut(s)	656, 1614
HphI	GGTGA	4 cut(s)	146, 346, 1676, 1834
Hpy166II	GTNNAC	5 cut(s)	790, 983, 1240, 1295, 1586
Hpy188I	TCNGA	8 cut(s)	233, 865, 921, 1185, 1357, 1600, 1651, 1680
Hpy188III	TCNNGA	7 cut(s)	31, 628, 701, 741, 1061, 1475, 1554
Hpy8I	GTNNAC	5 cut(s)	790, 983, 1240, 1295, 1586
Hpy99I	CGWCG	1 cut(s)	164
HpyAV	CCTTC	9 cut(s)	128, 313, 367, 377, 595, 1156, 1241, 1324, 1355
HpyCH4III	ACNGT	4 cut(s)	796, 869, 1244, 1511
HpyCH4IV	ACGT	1 cut(s)	569
HpyF10VI	GCNNNNNNNGC	7 cut(s)	77, 390, 587, 611, 883, 1331, 1501
HpyF3I	CTNAG	7 cut(s)	76, 591, 606, 765, 1345, 1390, 1679
HpySE526I	ACGT	1 cut(s)	569
Hsp92I	GRCGYC	2 cut(s)	569, 1366
KpnI	GGTACC	1 cut(s)	1314
Ksp22I	TGATCA	1 cut(s)	1221
KspAI	GTTAAC	2 cut(s)	790, 1240
Kzo9I	GATC	5 cut(s)	970, 1100, 1221, 1438, 1731
LguI	GCTCTTC	1 cut(s)	830
LmnI	GCTCC	2 cut(s)	234, 1551
Lsp1109I	GCAGC	4 cut(s)	67, 393, 873, 1726
LweI	GCATC	1 cut(s)	1760
MaeI	CTAG	3 cut(s)	1115, 1460, 1554
MaeII	ACGT	1 cut(s)	569
MaeIII	GTNAC	4 cut(s)	994, 1483, 1705, 1776
MalI	GATC	5 cut(s)	972, 1102, 1223, 1440, 1733
MboI	GATC	5 cut(s)	970, 1100, 1221, 1438, 1731
MfeI	CAATTG	1 cut(s)	1842
MflI	RGATCY	1 cut(s)	970
MhlI	GDGCHC	4 cut(s)	154, 231, 1027, 1528
MlsI	TGGCCA	1 cut(s)	189
MluCI	AATT	9 cut(s)	135, 417, 576, 646, 858, 1110, 1412, 1757, 1842
MluNI	TGGCCA	1 cut(s)	189
MlyI	GAGTC	3 cut(s)	492, 776, 947
MmeI	TCCRAC	3 cut(s)	545, 1709, 1747
Mox20I	TGGCCA	1 cut(s)	189
MscI	TGGCCA	1 cut(s)	189
MseI	TTAA	7 cut(s)	315, 575, 639, 789, 944, 1239, 1740
MslI	CAYNNNNRTG	1 cut(s)	426
Msp20I	TGGCCA	1 cut(s)	189
MspI	CCGG	2 cut(s)	656, 1614
MspR9I	CCNGG	3 cut(s)	524, 1615, 1719
MunI	CAATTG	1 cut(s)	1842
Mva1269I	GAATGC	1 cut(s)	901
MvaI	CCWGG	2 cut(s)	524, 1719
MwoI	GCNNNNNNNGC	7 cut(s)	77, 390, 587, 611, 883, 1331, 1501
NciI	CCSGG	1 cut(s)	1615
NdeI	CATATG	1 cut(s)	913
NdeII	GATC	5 cut(s)	970, 1100, 1221, 1438, 1731
NlaIV	GGNNCC	3 cut(s)	230, 335, 1312
NmuCI	GTSAC	2 cut(s)	1705, 1776
NspI	RCATGY	3 cut(s)	709, 1138, 1338
NspV	TTCGAA	1 cut(s)	695
PaeI	GCATGC	1 cut(s)	1338
PaeR7I	CTCGAG	1 cut(s)	1475
PagI	TCATGA	1 cut(s)	1060
PceI	AGGCCT	1 cut(s)	1458
PciI	ACATGT	2 cut(s)	705, 1134
PciSI	GCTCTTC	1 cut(s)	830
PctI	GAATGC	1 cut(s)	901
PfeI	GAWTC	4 cut(s)	398, 697, 733, 761
PkrI	GCNGC	5 cut(s)	82, 383, 654, 888, 1716
PleI	GAGTC	3 cut(s)	492, 776, 946
PpsI	GAGTC	3 cut(s)	492, 776, 946
PpuMI	RGGWCCY	1 cut(s)	333
PscI	ACATGT	2 cut(s)	705, 1134
PshBI	ATTAAT	1 cut(s)	1740
Psp5II	RGGWCCY	1 cut(s)	333
Psp6I	CCWGG	2 cut(s)	522, 1717
PspGI	CCWGG	2 cut(s)	522, 1717
PspN4I	GGNNCC	3 cut(s)	230, 335, 1312
PspPI	GGNCC	1 cut(s)	333
PspPPI	RGGWCCY	1 cut(s)	333
PsuI	RGATCY	1 cut(s)	970
RsaI	GTAC	2 cut(s)	798, 1312
RsaNI	GTAC	2 cut(s)	797, 1311
RseI	CAYNNNNRTG	1 cut(s)	426
SapI	GCTCTTC	1 cut(s)	830
SaqAI	TTAA	7 cut(s)	315, 575, 639, 789, 944, 1239, 1740
SatI	GCNGC	5 cut(s)	81, 382, 653, 887, 1715
Sau3AI	GATC	5 cut(s)	970, 1100, 1221, 1438, 1731
Sau96I	GGNCC	1 cut(s)	333
SchI	GAGTC	3 cut(s)	492, 776, 947
ScrFI	CCNGG	3 cut(s)	524, 1615, 1719
SduI	GDGCHC	4 cut(s)	154, 231, 1027, 1528
SfaNI	GCATC	1 cut(s)	1760
SfcI	CTRYAG	1 cut(s)	1723
Sfr274I	CTCGAG	1 cut(s)	1475
SfuI	TTCGAA	1 cut(s)	695
SinI	GGWCC	1 cut(s)	333
SlaI	CTCGAG	1 cut(s)	1475
SmiMI	CAYNNNNRTG	1 cut(s)	426
SmlI	CTYRAG	5 cut(s)	29, 371, 500, 1026, 1475
SmoI	CTYRAG	5 cut(s)	29, 371, 500, 1026, 1475
SphI	GCATGC	1 cut(s)	1338
Sse9I	AATT	9 cut(s)	135, 417, 576, 646, 858, 1110, 1412, 1757, 1842
SseBI	AGGCCT	1 cut(s)	1458
SsiI	CCGC	5 cut(s)	393, 581, 619, 652, 1684
SspI	AATATT	1 cut(s)	1374
SspMI	CTAG	3 cut(s)	1115, 1460, 1554
StuI	AGGCCT	1 cut(s)	1458
StyD4I	CCNGG	3 cut(s)	522, 1613, 1717
StyI	CCWWGG	2 cut(s)	890, 1318
TaaI	ACNGT	4 cut(s)	796, 869, 1244, 1511
TaiI	ACGT	1 cut(s)	572
TaqI	TCGA	5 cut(s)	695, 750, 1193, 1476, 1657
TasI	AATT	9 cut(s)	135, 417, 576, 646, 858, 1110, 1412, 1757, 1842
TatI	WGTACW	1 cut(s)	796
TauI	GCSGC	1 cut(s)	655
TfiI	GAWTC	4 cut(s)	398, 697, 733, 761
Tru1I	TTAA	7 cut(s)	315, 575, 639, 789, 944, 1239, 1740
Tru9I	TTAA	7 cut(s)	315, 575, 639, 789, 944, 1239, 1740
TscAI	CASTG	6 cut(s)	682, 799, 874, 1012, 1126, 1249
TseFI	GTSAC	2 cut(s)	1705, 1776
TseI	GCWGC	4 cut(s)	80, 381, 886, 1714
Tsp45I	GTSAC	2 cut(s)	1705, 1776
TspDTI	ATGAA	9 cut(s)	17, 212, 549, 1049, 1077, 1169, 1197, 1770, 1847
TspGWI	ACGGA	3 cut(s)	203, 775, 944
TspRI	CASTG	6 cut(s)	682, 799, 874, 1012, 1126, 1249
VpaK11BI	GGWCC	1 cut(s)	333
VspI	ATTAAT	1 cut(s)	1740
XapI	RAATTY	6 cut(s)	135, 417, 646, 858, 1110, 1757
XbaI	TCTAGA	1 cut(s)	1553
XceI	RCATGY	3 cut(s)	709, 1138, 1338
XhoI	CTCGAG	1 cut(s)	1475
XspI	CTAG	3 cut(s)	1115, 1460, 1554
ZraI	GACGTC	1 cut(s)	570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.