Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0005883

Physical Location & Seq

Genomic Coordinates Reverse (-)

14300036 .. 14306640

Gene Structure 6605 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3705 bp

ATGCTTCTGTTTATTCTAGAAGCACCTCCCAAATCAACGACTTCCGAATTACGAAAATACCCCTTACGTTTCTTCCAGCTCCGCCCCTGGCAATATATTCTCCGTGACACAGGTTTAGAACCTCTGTTCAAAAAATCAACAGGCTCTTCTCGTTCTCTCTCCGTCGTCTTCTTCCTCCATCAGAGTACCTCTCACCCCGAACTCCCAGATTTCGTCAGCTTGACACTAGGTACCACATTTATTGCCTGCTGTCAGTTTTGGATGGGAACCAAGGAGCATGACACTGTTTTTAAAGATGACGAGCCTACTACTATTGGCCACGATAATACTTCGTTGAGCCAGGTTGAGTCTTCAACAAGCGCAGTTCTTTGTGATTCAAATGTAAATATTGAAAGTAATGAAGTTAAAAGGTTAAGTGAGGTTGTCAGCAACATTAACAATACCCCATATATTTATAGACAAGATGTCGTGAGGAGCAAGAGTGGTATGACTGGGATTGTAACTGAGGTTGCTGGTGATTCAGATTCTGATAGTAGCATCATTGATGAGGAGGATGATGATGAAGATGATTATTACGATGATGATGAGAATGAGGAAGAAGGTGGTAATAACAATAATACTACTCATGGGAATAGTGACAAGAACAAAAATGGTAGTAATGATAAGACTGGTCCCCTTCCTGCTGACCAAGTTCGAGTACTTTGGATGGATGAGACAGAGTCAACCCAAAATATTAATGATGTAACGGTTGTTGATCGATCGTTCTTACATGGGGATTTTGTTGCTGCTGCTTCTGACCCAACTGGGCAAGTTGGTGTCGTGGTGGATGTCAATATCTCTGTTGATCGATTAGCTCCTGATGGGTCAGTCATAAAAGACCTTTCATCCAATGAATTAAAACGTGTGAGGGAGTTCACTGTGGGTGATTATGTGGTCCTTGGTCCCTGGCTAGGTAGGATTGATGATGTTTATGATAATGTGACAGTTTTGTTTGATGATGGTTCTGTGTGTAAAGTTATGAAAGCAGATCCGATGGACCTGAAACCAGTTTCCAAGAATATGCTTGAAGATGTGCATTTCCCTTACTACCCAGGGCAGCGTGTGAGGGCAAGGTCATCAGTTTTCAAGAATGCTAGGTGGCTATCTGGCTTGTGGAAACCTAATCGCGTGGAAGGTACTGTTACTAAGGTTACAGTTGCTTCAGTATTAATTTATTGGATAGCTTCTGCTGGCTGTGGGCCAGATTCTTCTACTGCACCTGCTAAAGAGCAGGTTCCCAAGAACTTGAAATTACTGTCTTGTTTTACGCATGCAACTTGGCAATTGGGTGATTGGTGTCTTCTCCCCTCATCAGTGTCATCGTCTTCCCTTCCTTTAGACAAGGGATCATCAGAATTAGAACTTAATGATTCTGTAAGCAGTGAGTTAGAACCTAGTCAAATGGGTAGTAAGTGTGATTCAGAAGAGAGTGCGCTAGATGAATCAAATGGAAATCATGAATCCATGGAAATCGATCCAGTATCTGTATTAGATGGAAACAATGGAAATACTGGAAGGAATACATCTGTTGAATCTAGTTCCTTTAATAGTTCATTGTCAGTTTCAAAGGAGCCTGTTCATGAAACTTGGCCTCTTCATCGCAAAAAAATCCGCAAAGTTGTGGTTAGGAGAGACAAGAAGGCACATAAGGAAGAAAATTTTGAAAGATCCTTTTTGATTGTCAATACCAAGACGACAGTTGACGTGGCATGGCAGGATGGCACAACAGAACTTAAACTGGACTCAAAAGATTTAATTCCGCTTGATAGTCCGGGTGACCACGAATTTGTTGCTGAACAGTATGTGGTGGAGAAGGCGACTGATGATGGTGATGATGCTTATGAAGCTAGGCATGTTGGGCTTGTGAAAAGTGTCAATGCCAAGGAGAGAACAGCTTGTGTAAGGTGGTTAAAGCCAGTTTCCAGACCAGAAGATCCTAGAGAGTTTGACAAAGAGGAAATTGTGAGCGTCTATGAGCTTGAGGGGCATCCGGATTATGATTACTGTTATGGGGATGTTGTTGTCAGATTATTGCCAGTTTTTGTCTCTGCACAAACTGCTTCAGGTACAGAATTCGATGAGGAACCAAAACAACAAAACGGACCATGCGAGCTTAGTTCTGCTTCTACAAAAAAAGATCTATCTAGTGATGAAGCTTGTTTGGACTTCTCAGATCTTTCTTGGGTTGGAAATATAACGGGTCTTAAGAATGGGGATATTGAAGTTACATGGGCTGACGGGATGGTTTCTATGGTTGGACCTCAAGCAATTTATGTTGTTGGCCGAGCTGATGATGATGACTCTATCGATGCTGGAAGTGATGTTAGTGATGCTGCTAGTTGGGAAACGGTTAATGATGATGAGACGCCTGCACTATGTGCCCCTGCGATCACAGAAGAGGAAGTCCAGCTACAAAATTCCTTTGGTATGAATTTTGAAGCAGAAGAAAGTGGAGAGAACAATTCTGGAAGGAACCCAGCATTGTCTGTTCCCTTGGCTGCACTCAGGTTCGTTACCAGACTGGCGCATGGTATATTCTCAAGGGGACAAAGGAATGTGGATTCAAATTCTTCGGATAATGAAGGTGAGGGCGAAGGTGAAGTTGAGCCTCGGGAAGTGGAAATTTCTCAGGGAAGAGATCATGGTGAAGATTCCAGCTCTCAGAATTCTAATGTAGTGGATCCTTATGGTCTAGAAATAAATAGGGCAGAAGAAGAGACAAATGTTACCCCACAGGCTGCAGAAATGTTGGATGCAGCAGAAGTTCTATGCAACTTAAACACTGAAGAATCAGATTCTATAGAATGCACTAAGGATGATGCTTGCAGTTTCAAACGCTTTGATATTGCCAAAGATCCTTTGGATCATCATTTCCTTTGTGCAGCTGGGCAGAATACCAGTGGAAGAAAGTGGTTGAAGAAGGTTCAGCAAGATTGGAACATCCTTCAAAATAACCTTCCCGATGGGATTTGTGTACGAGTGTATGAAGACCGAATGGATCTCTTGAGGGCAGTTATAGTTGGGGCTTATGGGACACCGTACCAAGATGGCCTCTTCTTTTTTGATTTTCACCTCCCTCCAGAGTACCCTGATGTTCCTCCGACAGCATACTATCATTCTGGTGGCTGGCGGATAAATCCAAATCTATATGAAGAAGGCAAAGTGTGTCTTAGTCTTTTGAATACTTGGACTGGCAGAGGGAATGAAGTATGGGATTCAAAGTCTTCTAGCATCCTTCAAGTTCTTGTTTCACTCCAGGGGTTGGTGCTAAATTCTAAGCCATATTTTAATGAAGCTGGCTATGATAAGCAGGTCGGGACGGCTGAAGGAGAGAAAAATTCATTGTCCTACAATGAGAATACTTTCTTACTGAACTGCAAGACAATGATGTATCTTATGCGGAGGCCACCAAAGGACTTTGAAGAGCTCATCAATGACCATTTCCGGAGGCGTGGTTATTATATCTTGAAGGCCTGTGATGCATATATGAAAGGCTATTTGATTGGTTCTCTCACTAAAGATGCCTCTCTTAGTGATAAGAGCGATGTAAATTCAACTTCGGTTGGTTTCAAGTTGATGTTAGCTAAGATTGTGCCAAAGCTTTTCTCGGCCCTGAGTGCGGTTGGAGCTAATTGTCAAGAATTTAAGCATCTGCAACAATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

1235

Amino Acids

136.79

Weight (kDa)

4.59

Isoelectric Point (pI)

41.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SH3-A_UBE2O	PF23048	151 - 251	6.2e-13	UBE2O, N-terminal SH3-A domain
tSH3-B_UBE2O	PF23046	307 - 437	4.9e-47	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	606 - 678	4.6e-20	UBE2O, SH3-B domain
SH3-C_UBE2O	PF23044	739 - 779	6.2e-07	UBE2O, ubiquitin binding SH3-C domain
UQ_con	PF00179	988 - 1118	4.2e-19	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16920 AT2G16920
fragaria_vesca	FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490
malus_domestica	MD05G1123200.v1.1 MD10G1126200.v1.1
prunus_persica	Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1
pyrus_communis	pycom05g11760 pycom10g10840
rosa_chinensis	RchiOBHm_Chr6g0278491
rosa_laevigata	RLG00000013224
rosa_multiflora	Rmu_sc0000258.1_g000006
rosa_roxburghii	Rroxscaffold_7G00189850 Rroxscaffold_7G00190340
rosa_rugosa	Rorug06G0117200 Rorug06G0117200
rosa_samantha	Rh6AG226500 Rh6BG230700 Rh6CG233800 Rh6DG225100
rosa_wichuraiana	Rw6G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1267
Acc36I	ACCTGC	3 cut(s)	1261, 1267, 3340
Acc65I	GGTACC	1 cut(s)	230
AccB1I	GGYRCC	1 cut(s)	230
AccB7I	CCANNNNNTGG	1 cut(s)	3301
AccII	CGCG	1 cut(s)	1167
AccIII	TCCGGA	2 cut(s)	2029, 3483
AciI	CCGC	6 cut(s)	82, 1651, 1799, 3169, 3439, 3659
AcoI	YGGCCR	2 cut(s)	316, 2320
AcuI	CTGAAG	4 cut(s)	1185, 2085, 2844, 3384
AcyI	GRCGYC	1 cut(s)	2405
AdeI	CACNNNGTG	1 cut(s)	2417
AfaI	GTAC	8 cut(s)	187, 232, 699, 1177, 2107, 3015, 3080, 3125
AfiI	CCNNNNNNNGG	3 cut(s)	950, 3301, 3658
AflII	CTTAAG	1 cut(s)	2243
AflIII	ACRYGT	1 cut(s)	901
AhdI	GACNNNNNGTC	1 cut(s)	464
AjiI	CACGTC	1 cut(s)	1744
AjnI	CCWGG	5 cut(s)	86, 339, 944, 1090, 3294
Alw21I	GWGCWC	1 cut(s)	3468
Alw26I	GTCTC	5 cut(s)	707, 1665, 2089, 2396, 2750
AlwNI	CAGNNNCTG	2 cut(s)	527, 1523
Ama87I	CYCGRG	1 cut(s)	2649
Aor13HI	TCCGGA	2 cut(s)	2029, 3483
AoxI	GGCC	8 cut(s)	316, 1238, 1628, 2320, 3088, 3443, 3510, 3648
ApeKI	GCWGC	8 cut(s)	785, 788, 1096, 2372, 2537, 2777, 2795, 2921
ArsI	GACNNNNNNTTYG	2 cut(s)	1808, 1840
AseI	ATTAAT	2 cut(s)	735, 1208
Asp700I	GAANNNNTTC	1 cut(s)	3401
Asp718I	GGTACC	1 cut(s)	230
AspLEI	GCGC	3 cut(s)	362, 1474, 2566
AspS9I	GGNCC	8 cut(s)	671, 934, 941, 1036, 1238, 2141, 2297, 3649
AsuC2I	CCSGG	1 cut(s)	1812
AvaI	CYCGRG	1 cut(s)	2649
AvaII	GGWCC	6 cut(s)	671, 934, 941, 1036, 2141, 2297
BaeGI	GKGCMC	1 cut(s)	2422
BalI	TGGCCA	1 cut(s)	318
BamHI	GGATCC	1 cut(s)	2719
BanI	GGYRCC	1 cut(s)	230
BanII	GRGCYC	1 cut(s)	3468
BarI	GAAGNNNNNNTAC	2 cut(s)	2433, 2465
BbsI	GAAGAC	6 cut(s)	160, 342, 1331, 1356, 3033, 3255
Bbv12I	GWGCWC	1 cut(s)	3468
BbvI	GCAGC	8 cut(s)	772, 775, 1108, 2359, 2524, 2764, 2807, 2933
BceAI	ACGGC	1 cut(s)	3375
BcgI	CGANNNNNNTGC	2 cut(s)	1811, 1845
BciT130I	CCWGG	5 cut(s)	88, 341, 946, 1092, 3296
BcnI	CCSGG	1 cut(s)	1812
BcoDI	GTCTC	5 cut(s)	707, 1665, 2089, 2396, 2750
BfmI	CTRYAG	2 cut(s)	2778, 2838
BfrI	CTTAAG	1 cut(s)	2243
BfuAI	ACCTGC	3 cut(s)	1261, 1267, 3340
BglII	AGATCT	2 cut(s)	2176, 2212
BisI	GCNGC	8 cut(s)	786, 789, 1097, 2373, 2538, 2778, 2796, 2922
BlsI	GCNGC	8 cut(s)	787, 790, 1098, 2374, 2539, 2779, 2797, 2923
BmcAI	AGTACT	1 cut(s)	699
Bme1390I	CCNGG	6 cut(s)	88, 341, 946, 1092, 1812, 3296
Bme18I	GGWCC	6 cut(s)	671, 934, 941, 1036, 2141, 2297
BmeRI	GACNNNNNGTC	1 cut(s)	464
BmeT110I	CYCGRG	1 cut(s)	2649
BmgBI	CACGTC	1 cut(s)	1744
BmgT120I	GGNCC	8 cut(s)	671, 934, 941, 1036, 1238, 2141, 2297, 3649
BmiI	GGNNCC	9 cut(s)	232, 268, 673, 943, 1275, 1611, 2124, 2513, 2721
BmrFI	CCNGG	6 cut(s)	88, 341, 946, 1092, 1812, 3296
BmrI	ACTGGG	2 cut(s)	501, 813
BmuI	ACTGGG	2 cut(s)	501, 813
BpiI	GAAGAC	6 cut(s)	160, 342, 1331, 1356, 3033, 3255
BplI	GAGNNNNNCTC	2 cut(s)	175, 207
BpmI	CTGGAG	2 cut(s)	3102, 3278
BpuEI	CTTGAG	4 cut(s)	2039, 2286, 2563, 3064
BpuMI	CCSGG	1 cut(s)	1812
Bsa29I	ATCGAT	4 cut(s)	757, 847, 1512, 2346
BsaHI	GRCGYC	1 cut(s)	2405
BsaWI	WCCGGW	2 cut(s)	2029, 3483
BsaXI	ACNNNNNCTCC	4 cut(s)	466, 496, 3478, 3508
Bsc4I	CCNNNNNNNGG	3 cut(s)	950, 3301, 3658
BseAI	TCCGGA	2 cut(s)	2029, 3483
BseBI	CCWGG	5 cut(s)	88, 341, 946, 1092, 3296
BseCI	ATCGAT	4 cut(s)	757, 847, 1512, 2346
BseLI	CCNNNNNNNGG	3 cut(s)	950, 3301, 3658
BseMII	CTCAG	6 cut(s)	495, 2223, 2557, 2681, 2714, 3644
BseRI	GAGGAG	2 cut(s)	487, 563
BseSI	GKGCMC	1 cut(s)	2422
BseXI	GCAGC	8 cut(s)	772, 775, 1108, 2359, 2524, 2764, 2807, 2933
BseYI	CCCAGC	2 cut(s)	2515, 2924
BsgI	GTGCAG	5 cut(s)	1239, 2073, 2394, 2523, 2940
Bsh1236I	CGCG	1 cut(s)	1167
Bsh1285I	CGRYCG	1 cut(s)	761
BshFI	GGCC	8 cut(s)	318, 1240, 1630, 2322, 3090, 3445, 3512, 3650
BshNI	GGYRCC	1 cut(s)	230
BshVI	ATCGAT	4 cut(s)	757, 847, 1512, 2346
BsiEI	CGRYCG	1 cut(s)	761
BsiHKAI	GWGCWC	1 cut(s)	3468
BsiHKCI	CYCGRG	1 cut(s)	2649
BsiSI	CCGG	3 cut(s)	1811, 2030, 3484
BslFI	GGGAC	5 cut(s)	657, 927, 2598, 3085, 3370
BslI	CCNNNNNNNGG	3 cut(s)	950, 3301, 3658
BsmAI	GTCTC	5 cut(s)	707, 1665, 2089, 2396, 2750
BsmBI	CGTCTC	1 cut(s)	2396
BsmFI	GGGAC	5 cut(s)	657, 927, 2598, 3085, 3370
BsmI	GAATGC	2 cut(s)	1135, 2849
BsnI	GGCC	8 cut(s)	318, 1240, 1630, 2322, 3090, 3445, 3512, 3650
BsoBI	CYCGRG	1 cut(s)	2649
Bsp1286I	GDGCHC	2 cut(s)	2422, 3468
Bsp13I	TCCGGA	2 cut(s)	2029, 3483
Bsp19I	CCATGG	1 cut(s)	1503
BspACI	CCGC	6 cut(s)	82, 1651, 1799, 3169, 3439, 3659
BspANI	GGCC	8 cut(s)	318, 1240, 1630, 2322, 3090, 3445, 3512, 3650
BspCNI	CTCAG	6 cut(s)	496, 2222, 2556, 2680, 2713, 3645
BspDI	ATCGAT	4 cut(s)	757, 847, 1512, 2346
BspEI	TCCGGA	2 cut(s)	2029, 3483
BspFNI	CGCG	1 cut(s)	1167
BspHI	TCATGA	2 cut(s)	1495, 1618
BspLI	GGNNCC	9 cut(s)	232, 268, 673, 943, 1275, 1611, 2124, 2513, 2721
BspMAI	CTGCAG	1 cut(s)	2782
BspMI	ACCTGC	3 cut(s)	1261, 1267, 3340
BspQI	GCTCTTC	2 cut(s)	151, 3456
BspT107I	GGYRCC	1 cut(s)	230
BspTI	CTTAAG	1 cut(s)	2243
BssNI	GRCGYC	1 cut(s)	2405
BssT1I	CCWWGG	5 cut(s)	270, 937, 1503, 1920, 2532
Bst2UI	CCWGG	5 cut(s)	88, 341, 946, 1092, 3296
Bst6I	CTCTTC	8 cut(s)	151, 1458, 1638, 2430, 2668, 2748, 3098, 3456
BstACI	GRCGYC	1 cut(s)	2405
BstAFI	CTTAAG	1 cut(s)	2243
BstAPI	GCANNNNNTGC	2 cut(s)	2096, 2417
BstC8I	GCNNGC	8 cut(s)	247, 1231, 1311, 2150, 2409, 2863, 3167, 3337
BstDSI	CCRYGG	1 cut(s)	1503
BstEII	GGTNACC	1 cut(s)	1814
BstFNI	CGCG	1 cut(s)	1167
BstHHI	GCGC	3 cut(s)	362, 1474, 2566
BstMAI	GTCTC	5 cut(s)	707, 1665, 2089, 2396, 2750
BstMCI	CGRYCG	1 cut(s)	761
BstMWI	GCNNNNNNNGC	8 cut(s)	1883, 1897, 2023, 2096, 2417, 3518, 3656, 3665
BstNI	CCWGG	5 cut(s)	88, 341, 946, 1092, 3296
BstNSI	RCATGY	2 cut(s)	1313, 1895
BstPI	GGTNACC	1 cut(s)	1814
BstSCI	CCNGG	6 cut(s)	86, 339, 944, 1090, 1810, 3294
BstSFI	CTRYAG	2 cut(s)	2778, 2838
BstSLI	GKGCMC	1 cut(s)	2422
BstUI	CGCG	1 cut(s)	1167
BstV1I	GCAGC	8 cut(s)	772, 775, 1108, 2359, 2524, 2764, 2807, 2933
BstV2I	GAAGAC	6 cut(s)	160, 342, 1331, 1356, 3033, 3255
BstX2I	RGATCY	8 cut(s)	1027, 1706, 1972, 2176, 2212, 2719, 2893, 3037
BstYI	RGATCY	8 cut(s)	1027, 1706, 1972, 2176, 2212, 2719, 2893, 3037
Bsu15I	ATCGAT	4 cut(s)	757, 847, 1512, 2346
BsuRI	GGCC	8 cut(s)	318, 1240, 1630, 2322, 3090, 3445, 3512, 3650
BsuTUI	ATCGAT	4 cut(s)	757, 847, 1512, 2346
BtgI	CCRYGG	1 cut(s)	1503
BtgZI	GCGATG	2 cut(s)	1622, 3597
BtrI	CACGTC	1 cut(s)	1744
BtsI	GCAGTG	1 cut(s)	1426
BtsIMutI	CAGTG	6 cut(s)	282, 915, 1359, 1426, 2820, 2944
BveI	ACCTGC	3 cut(s)	1261, 1267, 3340
Cac8I	GCNNGC	8 cut(s)	247, 1231, 1311, 2150, 2409, 2863, 3167, 3337
CaiI	CAGNNNCTG	2 cut(s)	527, 1523
CciI	TCATGA	2 cut(s)	1495, 1618
CfoI	GCGC	3 cut(s)	362, 1474, 2566
Cfr13I	GGNCC	8 cut(s)	671, 934, 941, 1036, 1238, 2141, 2297, 3649
ClaI	ATCGAT	4 cut(s)	757, 847, 1512, 2346
CseI	GACGC	2 cut(s)	1996, 2413
Csp6I	GTAC	8 cut(s)	186, 231, 698, 1176, 2106, 3014, 3079, 3124
CspCI	CAANNNNNGTGG	2 cut(s)	223, 258
CviQI	GTAC	8 cut(s)	186, 231, 698, 1176, 2106, 3014, 3079, 3124
DraI	TTTAAA	1 cut(s)	292
DraIII	CACNNNGTG	1 cut(s)	2417
DriI	GACNNNNNGTC	1 cut(s)	464
EaeI	YGGCCR	2 cut(s)	316, 2320
Eam1104I	CTCTTC	8 cut(s)	151, 1458, 1638, 2430, 2668, 2748, 3098, 3456
Eam1105I	GACNNNNNGTC	1 cut(s)	464
EarI	CTCTTC	8 cut(s)	151, 1458, 1638, 2430, 2668, 2748, 3098, 3456
EciI	GGCGGA	2 cut(s)	71, 3184
Ecl136II	GAGCTC	1 cut(s)	3466
Eco130I	CCWWGG	5 cut(s)	270, 937, 1503, 1920, 2532
Eco147I	AGGCCT	1 cut(s)	3512
Eco24I	GRGCYC	1 cut(s)	3468
Eco47I	GGWCC	6 cut(s)	671, 934, 941, 1036, 2141, 2297
Eco53kI	GAGCTC	1 cut(s)	3466
Eco57I	CTGAAG	4 cut(s)	1185, 2085, 2844, 3384
Eco88I	CYCGRG	1 cut(s)	2649
Eco91I	GGTNACC	1 cut(s)	1814
EcoICRI	GAGCTC	1 cut(s)	3466
EcoO65I	GGTNACC	1 cut(s)	1814
EcoRI	GAATTC	2 cut(s)	2111, 2704
EcoRII	CCWGG	5 cut(s)	86, 339, 944, 1090, 3294
EcoT14I	CCWWGG	5 cut(s)	270, 937, 1503, 1920, 2532
EcoT22I	ATGCAT	1 cut(s)	3523
EcoT38I	GRGCYC	1 cut(s)	3468
ErhI	CCWWGG	5 cut(s)	270, 937, 1503, 1920, 2532
Esp3I	CGTCTC	1 cut(s)	2396
FalI	AAGNNNNNCTT	2 cut(s)	3192, 3224
FaqI	GGGAC	5 cut(s)	657, 927, 2598, 3085, 3370
Fnu4HI	GCNGC	8 cut(s)	786, 789, 1097, 2373, 2538, 2778, 2796, 2922
FriOI	GRGCYC	1 cut(s)	3468
Fsp4HI	GCNGC	8 cut(s)	786, 789, 1097, 2373, 2538, 2778, 2796, 2922
GlaI	GCGC	3 cut(s)	361, 1473, 2565
GluI	GCNGC	8 cut(s)	786, 789, 1097, 2373, 2538, 2778, 2796, 2922
GsaI	CCCAGC	2 cut(s)	2519, 2928
GsuI	CTGGAG	2 cut(s)	3102, 3278
HaeIII	GGCC	8 cut(s)	318, 1240, 1630, 2322, 3090, 3445, 3512, 3650
HapII	CCGG	3 cut(s)	1811, 2030, 3484
HgaI	GACGC	2 cut(s)	1996, 2413
HhaI	GCGC	3 cut(s)	362, 1474, 2566
Hin1I	GRCGYC	1 cut(s)	2405
Hin6I	GCGC	3 cut(s)	360, 1472, 2564
HinP1I	GCGC	3 cut(s)	360, 1472, 2564
HincII	GTYRAC	2 cut(s)	723, 1741
HindII	GTYRAC	2 cut(s)	723, 1741
HindIII	AAGCTT	2 cut(s)	2193, 3638
HpaII	CCGG	3 cut(s)	1811, 2030, 3484
Hpy166II	GTNNAC	4 cut(s)	723, 915, 1741, 3014
Hpy8I	GTNNAC	4 cut(s)	723, 915, 1741, 3014
Hpy99I	CGWCG	1 cut(s)	167
HpyCH4IV	ACGT	3 cut(s)	67, 901, 1743
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1883, 1897, 2023, 2096, 2417, 3518, 3656, 3665
HpySE526I	ACGT	3 cut(s)	67, 901, 1743
Hsp92I	GRCGYC	1 cut(s)	2405
HspAI	GCGC	3 cut(s)	360, 1472, 2564
Kpn2I	TCCGGA	2 cut(s)	2029, 3483
KpnI	GGTACC	1 cut(s)	234
LguI	GCTCTTC	2 cut(s)	151, 3456
LmnI	GCTCC	6 cut(s)	84, 274, 474, 859, 1609, 3665
Lsp1109I	GCAGC	8 cut(s)	772, 775, 1108, 2359, 2524, 2764, 2807, 2933
MaeII	ACGT	3 cut(s)	67, 901, 1743
MfeI	CAATTG	1 cut(s)	1322
MflI	RGATCY	8 cut(s)	1027, 1706, 1972, 2176, 2212, 2719, 2893, 3037
MhlI	GDGCHC	2 cut(s)	2422, 3468
MlsI	TGGCCA	1 cut(s)	318
MluNI	TGGCCA	1 cut(s)	318
MlyI	GAGTC	4 cut(s)	356, 728, 1775, 2333
MmeI	TCCRAC	5 cut(s)	2206, 2275, 2769, 3164, 3643
Mox20I	TGGCCA	1 cut(s)	318
Mph1103I	ATGCAT	1 cut(s)	3523
MroI	TCCGGA	2 cut(s)	2029, 3483
MroXI	GAANNNNTTC	1 cut(s)	3401
MscI	TGGCCA	1 cut(s)	318
MslI	CAYNNNNRTG	1 cut(s)	3159
Msp20I	TGGCCA	1 cut(s)	318
MspA1I	CMGCKG	1 cut(s)	2924
MspCI	CTTAAG	1 cut(s)	2243
MspI	CCGG	3 cut(s)	1811, 2030, 3484
MspR9I	CCNGG	6 cut(s)	88, 341, 946, 1092, 1812, 3296
MunI	CAATTG	1 cut(s)	1322
Mva1269I	GAATGC	2 cut(s)	1135, 2849
MvaI	CCWGG	5 cut(s)	88, 341, 946, 1092, 3296
MvnI	CGCG	1 cut(s)	1167
MwoI	GCNNNNNNNGC	8 cut(s)	1883, 1897, 2023, 2096, 2417, 3518, 3656, 3665
NciI	CCSGG	1 cut(s)	1812
NcoI	CCATGG	1 cut(s)	1503
NlaIV	GGNNCC	9 cut(s)	232, 268, 673, 943, 1275, 1611, 2124, 2513, 2721
NmeAIII	GCCGAG	2 cut(s)	2348, 3626
NmuCI	GTSAC	4 cut(s)	104, 635, 979, 1814
NsiI	ATGCAT	1 cut(s)	3523
NspI	RCATGY	2 cut(s)	1313, 1895
PaeI	GCATGC	1 cut(s)	1313
PagI	TCATGA	2 cut(s)	1495, 1618
PaqCI	CACCTGC	1 cut(s)	1267
PasI	CCCWGGG	1 cut(s)	1091
PceI	AGGCCT	1 cut(s)	3512
PciSI	GCTCTTC	2 cut(s)	151, 3456
PcsI	WCGNNNNNNNCGW	1 cut(s)	2145
PctI	GAATGC	2 cut(s)	1135, 2849
PdmI	GAANNNNTTC	1 cut(s)	3401
PflFI	GACNNNGTC	1 cut(s)	718
PflMI	CCANNNNNTGG	1 cut(s)	3301
PkrI	GCNGC	8 cut(s)	787, 790, 1098, 2374, 2539, 2779, 2797, 2923
Ple19I	CGATCG	1 cut(s)	761
PleI	GAGTC	4 cut(s)	355, 727, 1775, 2333
PpsI	GAGTC	4 cut(s)	355, 727, 1775, 2333
PshBI	ATTAAT	2 cut(s)	735, 1208
Psp124BI	GAGCTC	1 cut(s)	3468
Psp6I	CCWGG	5 cut(s)	86, 339, 944, 1090, 3294
PspEI	GGTNACC	1 cut(s)	1814
PspFI	CCCAGC	2 cut(s)	2515, 2924
PspGI	CCWGG	5 cut(s)	86, 339, 944, 1090, 3294
PspN4I	GGNNCC	9 cut(s)	232, 268, 673, 943, 1275, 1611, 2124, 2513, 2721
PspPI	GGNCC	8 cut(s)	671, 934, 941, 1036, 1238, 2141, 2297, 3649
PstI	CTGCAG	1 cut(s)	2782
PstNI	CAGNNNCTG	2 cut(s)	527, 1523
PsuI	RGATCY	8 cut(s)	1027, 1706, 1972, 2176, 2212, 2719, 2893, 3037
PsyI	GACNNNGTC	1 cut(s)	718
PvuI	CGATCG	1 cut(s)	761
PvuII	CAGCTG	1 cut(s)	2924
RsaI	GTAC	8 cut(s)	187, 232, 699, 1177, 2107, 3015, 3080, 3125
RsaNI	GTAC	8 cut(s)	186, 231, 698, 1176, 2106, 3014, 3079, 3124
RseI	CAYNNNNRTG	1 cut(s)	3159
SacI	GAGCTC	1 cut(s)	3468
SapI	GCTCTTC	2 cut(s)	151, 3456
SatI	GCNGC	8 cut(s)	786, 789, 1097, 2373, 2538, 2778, 2796, 2922
Sau96I	GGNCC	8 cut(s)	671, 934, 941, 1036, 1238, 2141, 2297, 3649
ScaI	AGTACT	1 cut(s)	699
SchI	GAGTC	4 cut(s)	356, 728, 1775, 2333
ScrFI	CCNGG	6 cut(s)	88, 341, 946, 1092, 1812, 3296
SduI	GDGCHC	2 cut(s)	2422, 3468
SfcI	CTRYAG	2 cut(s)	2778, 2838
SinI	GGWCC	6 cut(s)	671, 934, 941, 1036, 2141, 2297
SmiMI	CAYNNNNRTG	1 cut(s)	3159
SmlI	CTYRAG	5 cut(s)	2018, 2243, 2301, 2578, 3043
SmoI	CTYRAG	5 cut(s)	2018, 2243, 2301, 2578, 3043
SphI	GCATGC	1 cut(s)	1313
SseBI	AGGCCT	1 cut(s)	3512
SsiI	CCGC	6 cut(s)	82, 1651, 1799, 3169, 3439, 3659
SspI	AATATT	2 cut(s)	388, 733
SstI	GAGCTC	1 cut(s)	3468
StuI	AGGCCT	1 cut(s)	3512
StyD4I	CCNGG	6 cut(s)	86, 339, 944, 1090, 1810, 3294
StyI	CCWWGG	5 cut(s)	270, 937, 1503, 1920, 2532
TaiI	ACGT	3 cut(s)	70, 904, 1746
TaqI	TCGA	6 cut(s)	694, 757, 847, 1512, 2115, 2346
TaqII	GACCGA	1 cut(s)	3045
TatI	WGTACW	1 cut(s)	697
TscAI	CASTG	6 cut(s)	289, 922, 1359, 1426, 2827, 2944
TseFI	GTSAC	4 cut(s)	104, 635, 979, 1814
TseI	GCWGC	8 cut(s)	785, 788, 1096, 2372, 2537, 2777, 2795, 2921
Tsp45I	GTSAC	4 cut(s)	104, 635, 979, 1814
TspGWI	ACGGA	3 cut(s)	92, 151, 2154
TspRI	CASTG	6 cut(s)	289, 922, 1359, 1426, 2827, 2944
Tth111I	GACNNNGTC	1 cut(s)	718
Van91I	CCANNNNNTGG	1 cut(s)	3301
Vha464I	CTTAAG	1 cut(s)	2243
VpaK11BI	GGWCC	6 cut(s)	671, 934, 941, 1036, 2141, 2297
VspI	ATTAAT	2 cut(s)	735, 1208
XbaI	TCTAGA	2 cut(s)	16, 2731
XceI	RCATGY	2 cut(s)	1313, 1895
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2896
XmnI	GAANNNNTTC	1 cut(s)	3401
ZrmI	AGTACT	1 cut(s)	699
Zsp2I	ATGCAT	1 cut(s)	3523

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.