Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007674

Physical Location & Seq

Genomic Coordinates Forward (+)

16141589 .. 16145692

Gene Structure 4104 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3408 bp

ATGCTCACCACAATGCCCACCTACTTCATCTTCCTCCTGCTCTTCTTATCCTCTCCACTCACTGCATCTCCAAGGCCAAACTATAGAGTCCTACACCAGCCATTTCTGCCTCAGGAATCTTCTTCGCCGCCGCCCACTCACCCACCATCTCCATCCCCGCCTTCCCCTCCTCCGAACCCAACTCCCCCAAAGTATCCATTTTCCAGTTCCAATTCTCCCCCTCCCAATTCCCCATTTTTCCCATCCAACCCTTCCCCTCCGCCTCCCCCTCCCGCCCCAGCCACCGCCTTCGCCTCATTCCCCGCCAACATCTCCTCCCTCATCGTCCCCCAGCCCACCAAAAACTCCAGCTCCCACAAGCTCCTCGGCCTCACTATCGCCGCCGTGCTCTCCGCCGTCGTCGTGGTGGCCGTTGCCGCATTCTTCCTTTACCGTCGCAGACGGCACAACAGCCGCAACTTCCCCGACGAGGATGAGGATGAGAAGTCGTATCTTAGATCCGAACACAGCAACAGGCTGTTACAGGCGCCCAATCTCGGCCAGTCCTACAGTGGGACCCACAAGCTCCGGACCGGCTCCTCCACCAGCTCCGAGTTCCTTTACCTGGGCACATTGGTCAATTCACGCTCACTTGAGGAGAGAGTCGACTCGGGCGGGAGTGTTGGTCGAAGCGCCGAATTAGAAACTCGGAAAGTCGACTCACCGGACCTCCAGCCGCTTCCGCCGCTTTCTCGGCAGATCTCGAGGCTGGGCCGTAGCGAGAATGCCAAACTGGGTTCGACTCAGGACCGAGACGATGAGGAAGAGGAAGAGGAAGAAGAGTTCTACTCCCCCAGAGGGTCTTCGGGCGATCGCGAGAGCTCCAACGGAAACGGATCCGGGTCCAGGAGAATGTTGACGGCGGTAGCGGGTGGCGTATTTGACCGAAGAAGCAGCGAAACCACGTCGTGTTCTTGCTCGTCTTCCGAATTGGGTTCACCGCCTCGATCACACTCTATCAGTCTCTCGCCTCCGGTGACTTTGAGTCCCAACAGAAGACCAGAAGAGCCCAAACTGCCGGAGCCCTCAGCCACGCGTTATACGACGCCGTTTGGAGACGTCAATGTACGGTCTCCGTCGTTGACTCCACTGTCTTCGCCGGAGAGAGCTGCTGACGAATACCCATCTGCATCGGACCGAAACGGAGGGTCTCCGTCGATGTCCTCGTTATCTTCGTCGCCGGAGAGAGGATTGGAGAAGACCCCAGATGCACCGCTAAAAGTTTCAGTCGTTTCGGATCAAAGTTCGCCGATTTCTTCACCGGAGAGAGATTTCGGAAACAACCCACATGCATCATCGAAAGTTTCGGTAGTTACGGATCAAAGTTCGCTGATTTCGTCACCGGAGAGACATTTCGGAATCAATCCAGATGCATTATCGAAAGTTTCGGTGGTTTCGGGTCAGAGTTCTCCTATTTCTTCACCGGAATTAAATTTCGGAAACAACGCAGATGGGTTAACGAAAGTTACGGCCTTTCCGGATCGGAATGATCAGTCCCCTTCCCCATTATCACATTGTTCATCGTCACCGGAGAGAGAATCAGACGGTTCTGATTCAAAAGCCAAGTCGTTTTCACCTTCTATGTCACAACTGAGAGGTTTGGAGAACTCAGCTGCCTCACCAAGAATTTCAAATGCTTCGGATCGAGCATTTATTCATTTGGATCCCAAAATGCAGTCTCTTTCATCATCTTCATCGTCTTCGTTGTCGAATTCACCGGAGAGAGCGTTTAGTGTTGGTTTAAAGCAGCCTCTTTCGGTTCCTCCACCGCCACCGCCAATGCCTCCTTCACTGAGACTCTGGGAGACTCCAAGTCCTAGAACACCAGTTGGCCAAGTGATCTCTAGGCCTCCAGCTCTCATACCTCCTTCAAGACCTTTTGTGATTCAAAACCCGGGAAAGGTCTCTGTTTCGCCTGTGGAGTTGCCACCAAGTTCTACGCCGTTGGAGCCGATTGAGGAGACTCCGAAACCCAAGTTGAAGCCATTGCATTGGGATAAAGTTAGAGCAAGCTCCGACCGCGAAATGGTGTGGGATCAGCTTAGATCAAGCTCTTTCAAATTGAATGAAGAAATGATAGAGACATTGTTTGTTGTAAAAACACCGAACCCGAATCCAAAAGAAACGACCCCACGAGCGGTTCTTCCCTCACTGAACCAAGAGAACAGAGTGCTTGATCCTAAAAAGTCCCAAAATATTGCAATCTCGCTAAGGGCGCTCAATGTGACCATAGAGGAAGTTTGCGAAGCTTTATTAGAAGGAAACTCGGATGCTCTTGGAACTGAATTACTTGAAAGTCTAATGAAGATGGCTCCAACCAAAGAAGAAGAACGTAAGCTGAAGGAATACAAAGACGACTCACCAGTGAAGCTTGGTCCTGCTGAGAGATTTTTGAAAGAGCTGCTTGACGTTCCATTTGCATTTAGAAGGATTGATGCAATGCTTTACGTGACTAATTTTGAGTCTGAGATCGAGTACCTTAAAAAATCGTTTGAAACTCTAGAGGCTGCCTGTGAAGAATTGAGAAACAGCAGGATGTTCTTGAAGCTTCTGGAAGCTGTTCTAAAGACTGGAAACCGGATGAATGTTGGGACAAACCGTGGTGATGCCCATGCATTCAAACTGGACACACTCCTCAAGCTGGTTGACGTTAAGGGGGCAGATGGGAAAACAACACTCCTGCATTTTGTCGTACAAGAAATCATAAGAACTGAAGGTTCTCGTCTCACTGGTGGCAATCAAATTTCAAACCCAACTTTGAGTGATGATGTCAAGTGCAGGAGGCTTGGCCTGCAAGTTGTTTCAGCTCTCAGTTCAGACCTCAGTAACGTGAAGAAAGCTGCTGCCATGGATTTTGATGTGCTTAGCAGTGATGTTTCCAAGCTTTCTAAAGGAATTAGCAACATTGCAGAGGTGGTGCAACTGAATGAGACAACGGTATCAGATGATAGCAGGCGGAAATTTTCAGAGTTGATGAACATGTTCATGAAATTGGCTGAAGAGGAGATTATAAGACTTCAAGCTCAAGAAAGTGTTGCACTGTCCCTGGTGAAGGAGATTACAGAGTACTTCCATGGAAACTCTGCTAGGGAAGAAGCTCACCCGTTTAGAATCTTCACGGTGGTCAGAGACTTTCTTACAGTTCTTGATCGGGTATGCAAGGAAGTTGGTATGATAAATGAGCGAACCCAAGTTAGTACCGCCCACAAATTTCCAGTTCCAGTAAATCCAATGCTACCACAAATAGTTCCGGTAAATCCAATGCTGCCACAAGTAGTTCCAGTCAATCCAATGCCACCACAAGTACTTCCTGGAATTCATGAGTTGAGGCGGTACGGTTCGTCCGATGATGAGAGCACAGCTCCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0030029 GO:0030036 GO:0030312 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0030838 GO:0031333 GO:0031334 GO:0032271 GO:0032272 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051016 GO:0051128 GO:0051129 GO:0051130 GO:0051493 GO:0051494 GO:0051495 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

1136

Amino Acids

123.87

Weight (kDa)

6.03

Isoelectric Point (pI)

72.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	667 - 1062	6.5e-122	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007674)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25500
fragaria_vesca	FvH4_2g17360 FvH4_2g17360 FvH4_2g17360
malus_domestica	MD05G1152500.v1.1 MD10G1147000.v1.1
prunus_persica	Prupe.8G185400_v2.0.a1
pyrus_communis	pycom05g14690 pycom10g12670
rosa_chinensis	RchiOBHm_Chr6g0281991
rosa_laevigata	RLG00000012921
rosa_multiflora	Rmu_sc0000569.1_g000001 Rmu_sc0001255.1_g000001 Rmu_sc0011823.1_g000001
rosa_roxburghii	Rroxscaffold_7G00186650
rosa_rugosa	Rorug06G0144700
rosa_samantha	Rh4CG162200 Rh4CG270300 Rh6AG257900 Rh6BG260900 Rh6CG259800 Rh6DG252200 Rh7AG416400
rosa_wichuraiana	Rw6G022300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3050
AasI	GACNNNNNNGTC	1 cut(s)	1129
AatII	GACGTC	1 cut(s)	1101
AccB1I	GGYRCC	1 cut(s)	526
AccBSI	CCGCTC	1 cut(s)	2177
AccI	GTMKAC	2 cut(s)	645, 696
AccII	CGCG	3 cut(s)	855, 1075, 2061
AccIII	TCCGGA	2 cut(s)	567, 1516
AcoI	YGGCCR	3 cut(s)	408, 538, 1870
AcsI	RAATTY	7 cut(s)	1471, 1665, 1750, 2781, 2999, 3248, 3354
AcuI	CTGAAG	3 cut(s)	2399, 2772, 3057
AcyI	GRCGYC	3 cut(s)	527, 1085, 1098
AdeI	CACNNNGTG	1 cut(s)	948
AfaI	GTAC	7 cut(s)	1107, 2516, 2733, 3107, 3238, 3345, 3374
AflIII	ACRYGT	2 cut(s)	1073, 3018
AhdI	GACNNNNNGTC	2 cut(s)	1023, 1851
AjiI	CACGTC	1 cut(s)	945
AjnI	CCWGG	4 cut(s)	603, 884, 3084, 3349
AjuI	GAANNNNNNNTTGG	2 cut(s)	1810, 1842
AloI	GAACNNNNNNTCC	2 cut(s)	806, 838
Alw21I	GWGCWC	3 cut(s)	390, 863, 3398
Ama87I	CYCGRG	3 cut(s)	649, 742, 1933
Aor13HI	TCCGGA	2 cut(s)	567, 1516
AoxI	GGCC	9 cut(s)	74, 367, 408, 538, 751, 1509, 1870, 1886, 2827
ApeKI	GCWGC	9 cut(s)	933, 1148, 1652, 1786, 2440, 2546, 2879, 2882, 3304
ApoI	RAATTY	7 cut(s)	1471, 1665, 1750, 2781, 2999, 3248, 3354
ArsI	GACNNNNNNTTYG	4 cut(s)	1589, 1621, 2438, 2470
AsiSI	GCGATCGC	1 cut(s)	853
Asp700I	GAANNNNTTC	2 cut(s)	2598, 3020
AspLEI	GCGC	3 cut(s)	529, 674, 2257
AspS9I	GGNCC	8 cut(s)	555, 570, 706, 751, 787, 882, 1174, 2414
AsuC2I	CCSGG	3 cut(s)	880, 1934, 1935
AvaI	CYCGRG	3 cut(s)	649, 742, 1933
AvaII	GGWCC	7 cut(s)	555, 570, 706, 787, 882, 1174, 2414
AxyI	CCTNAGG	1 cut(s)	111
BaeGI	GKGCMC	1 cut(s)	611
BaeI	ACNNNNGTAYC	2 cut(s)	2961, 2994
BalI	TGGCCA	1 cut(s)	1872
BamHI	GGATCC	2 cut(s)	875, 1702
BanI	GGYRCC	1 cut(s)	526
BanII	GRGCYC	3 cut(s)	863, 1050, 1065
BauI	CACGAG	1 cut(s)	2172
BbsI	GAAGAC	6 cut(s)	834, 954, 1042, 1125, 1244, 1731
Bbv12I	GWGCWC	3 cut(s)	390, 863, 3398
BbvCI	CCTCAGC	1 cut(s)	1066
BbvI	GCAGC	9 cut(s)	945, 1135, 1639, 1798, 2427, 2533, 2866, 2869, 3291
BccI	CCATC	7 cut(s)	154, 160, 250, 1171, 1484, 2341, 2696
BceAI	ACGGC	9 cut(s)	368, 380, 395, 458, 738, 915, 1072, 1524, 1966
BciT130I	CCWGG	4 cut(s)	605, 886, 3086, 3351
BciVI	GTATCC	1 cut(s)	204
BclI	TGATCA	1 cut(s)	1528
BcnI	CCSGG	3 cut(s)	880, 1934, 1935
BfaI	CTAG	4 cut(s)	1857, 1884, 2540, 3126
BfmI	CTRYAG	2 cut(s)	82, 547
BfoI	RGCGCY	3 cut(s)	530, 675, 2258
BfuI	GTATCC	1 cut(s)	204
BglII	AGATCT	1 cut(s)	738
BlpI	GCTNAGC	1 cut(s)	2903
BmcAI	AGTACT	2 cut(s)	3107, 3345
Bme1390I	CCNGG	7 cut(s)	605, 880, 886, 1934, 1935, 3086, 3351
Bme18I	GGWCC	7 cut(s)	555, 570, 706, 787, 882, 1174, 2414
BmeRI	GACNNNNNGTC	2 cut(s)	1023, 1851
BmeT110I	CYCGRG	3 cut(s)	649, 742, 1933
BmgBI	CACGTC	1 cut(s)	945
BmgT120I	GGNCC	8 cut(s)	555, 570, 706, 751, 787, 882, 1174, 2414
BmrFI	CCNGG	7 cut(s)	605, 880, 886, 1934, 1935, 3086, 3351
BmrI	ACTGGG	1 cut(s)	782
BmsI	GCATC	8 cut(s)	74, 1178, 1237, 1340, 1399, 2299, 2464, 2635
BmuI	ACTGGG	1 cut(s)	782
BpiI	GAAGAC	6 cut(s)	834, 954, 1042, 1125, 1244, 1731
BplI	GAGNNNNNCTC	3 cut(s)	812, 844, 3385
BpmI	CTGGAG	3 cut(s)	331, 695, 1875
Bpu10I	CCTNAGC	2 cut(s)	1066, 2249
Bpu1102I	GCTNAGC	1 cut(s)	2903
BpuEI	CTTGAG	3 cut(s)	653, 2660, 3048
BpuMI	CCSGG	3 cut(s)	880, 1934, 1935
BsaAI	YACGTR	1 cut(s)	2488
BsaHI	GRCGYC	3 cut(s)	527, 1085, 1098
BsaI	GGTCTC	3 cut(s)	1116, 1194, 1948
BsaJI	CCNNGG	8 cut(s)	71, 364, 604, 1933, 2638, 2886, 3084, 3112
Bse118I	RCCGGY	1 cut(s)	572
Bse21I	CCTNAGG	1 cut(s)	111
Bse3DI	GCAATG	3 cut(s)	2024, 2484, 2943
BseAI	TCCGGA	2 cut(s)	567, 1516
BseBI	CCWGG	4 cut(s)	605, 886, 3086, 3351
BseDI	CCNNGG	8 cut(s)	71, 364, 604, 1933, 2638, 2886, 3084, 3112
BseGI	GGATG	7 cut(s)	152, 242, 478, 484, 2314, 2580, 2625
BseMI	GCAATG	3 cut(s)	2024, 2484, 2943
BseRI	GAGGAG	8 cut(s)	159, 304, 353, 568, 650, 2012, 2663, 3056
BseSI	GKGCMC	1 cut(s)	611
BseXI	GCAGC	9 cut(s)	945, 1135, 1639, 1798, 2427, 2533, 2866, 2869, 3291
BseYI	CCCAGC	3 cut(s)	277, 330, 748
BsgI	GTGCAG	1 cut(s)	2836
Bsh1236I	CGCG	3 cut(s)	855, 1075, 2061
Bsh1285I	CGRYCG	2 cut(s)	853, 2059
BshFI	GGCC	9 cut(s)	76, 369, 410, 540, 753, 1511, 1872, 1888, 2829
BshNI	GGYRCC	1 cut(s)	526
BsiEI	CGRYCG	2 cut(s)	853, 2059
BsiHKAI	GWGCWC	3 cut(s)	390, 863, 3398
BsiHKCI	CYCGRG	3 cut(s)	649, 742, 1933
BslFI	GGGAC	7 cut(s)	311, 568, 1011, 1519, 2212, 2644, 3067
BsmBI	CGTCTC	3 cut(s)	786, 1089, 2768
BsmFI	GGGAC	7 cut(s)	311, 568, 1011, 1519, 2212, 2644, 3067
BsmI	GAATGC	3 cut(s)	419, 769, 2654
BsnI	GGCC	9 cut(s)	76, 369, 410, 540, 753, 1511, 1872, 1888, 2829
Bso31I	GGTCTC	3 cut(s)	1116, 1194, 1948
BsoBI	CYCGRG	3 cut(s)	649, 742, 1933
Bsp1286I	GDGCHC	6 cut(s)	390, 611, 863, 1050, 1065, 3398
Bsp13I	TCCGGA	2 cut(s)	567, 1516
Bsp1720I	GCTNAGC	1 cut(s)	2903
Bsp19I	CCATGG	2 cut(s)	2886, 3112
Bsp68I	TCGCGA	1 cut(s)	855
BspANI	GGCC	9 cut(s)	76, 369, 410, 540, 753, 1511, 1872, 1888, 2829
BspEI	TCCGGA	2 cut(s)	567, 1516
BspFNI	CGCG	3 cut(s)	855, 1075, 2061
BspHI	TCATGA	2 cut(s)	3024, 3358
BspQI	GCTCTTC	2 cut(s)	47, 1038
BspT107I	GGYRCC	1 cut(s)	526
BspTNI	GGTCTC	3 cut(s)	1116, 1194, 1948
BsrBI	CCGCTC	1 cut(s)	2177
BsrDI	GCAATG	3 cut(s)	2024, 2484, 2943
BsrFI	RCCGGY	1 cut(s)	572
BssAI	RCCGGY	1 cut(s)	572
BssECI	CCNNGG	8 cut(s)	71, 364, 604, 1933, 2638, 2886, 3084, 3112
BssNI	GRCGYC	3 cut(s)	527, 1085, 1098
BssSI	CACGAG	1 cut(s)	2172
BssT1I	CCWWGG	3 cut(s)	71, 2886, 3112
Bst2BI	CACGAG	1 cut(s)	2172
Bst2UI	CCWGG	4 cut(s)	605, 886, 3086, 3351
Bst6I	CTCTTC	6 cut(s)	47, 798, 804, 813, 1038, 3033
BstACI	GRCGYC	3 cut(s)	527, 1085, 1098
BstBAI	YACGTR	1 cut(s)	2488
BstC8I	GCNNGC	3 cut(s)	2050, 2831, 2993
BstDSI	CCRYGG	3 cut(s)	2638, 2886, 3112
BstENI	CCTNNNNNAGG	1 cut(s)	3089
BstF5I	GGATG	7 cut(s)	152, 242, 478, 484, 2314, 2580, 2625
BstFNI	CGCG	3 cut(s)	855, 1075, 2061
BstH2I	RGCGCY	3 cut(s)	530, 675, 2258
BstHHI	GCGC	3 cut(s)	529, 674, 2257
BstMCI	CGRYCG	2 cut(s)	853, 2059
BstNI	CCWGG	4 cut(s)	605, 886, 3086, 3351
BstNSI	RCATGY	2 cut(s)	1331, 3022
BstSCI	CCNGG	7 cut(s)	603, 878, 884, 1932, 1933, 3084, 3349
BstSFI	CTRYAG	2 cut(s)	82, 547
BstSLI	GKGCMC	1 cut(s)	611
BstUI	CGCG	3 cut(s)	855, 1075, 2061
BstV1I	GCAGC	9 cut(s)	945, 1135, 1639, 1798, 2427, 2533, 2866, 2869, 3291
BstV2I	GAAGAC	6 cut(s)	834, 954, 1042, 1125, 1244, 1731
BstX2I	RGATCY	4 cut(s)	497, 738, 875, 1702
BstXI	CCANNNNNNTGG	1 cut(s)	2031
BstYI	RGATCY	4 cut(s)	497, 738, 875, 1702
Bsu36I	CCTNAGG	1 cut(s)	111
BsuI	GTATCC	1 cut(s)	204
BsuRI	GGCC	9 cut(s)	76, 369, 410, 540, 753, 1511, 1872, 1888, 2829
BtgI	CCRYGG	3 cut(s)	2638, 2886, 3112
BtrI	CACGTC	1 cut(s)	945
BtsCI	GGATG	7 cut(s)	152, 242, 478, 484, 2314, 2580, 2625
BtsI	GCAGTG	2 cut(s)	60, 2914
BtsIMutI	CAGTG	9 cut(s)	60, 556, 1127, 1829, 2189, 2409, 2766, 2914, 3077
BtuMI	TCGCGA	1 cut(s)	855
Cac8I	GCNNGC	3 cut(s)	2050, 2831, 2993
CciI	TCATGA	2 cut(s)	3024, 3358
CfoI	GCGC	3 cut(s)	529, 674, 2257
Cfr10I	RCCGGY	1 cut(s)	572
Cfr13I	GGNCC	8 cut(s)	555, 570, 706, 751, 787, 882, 1174, 2414
Cfr9I	CCCGGG	1 cut(s)	1933
CpoI	CGGWCCG	2 cut(s)	570, 1174
CseI	GACGC	1 cut(s)	1093
Csp6I	GTAC	7 cut(s)	1106, 2515, 2732, 3106, 3237, 3344, 3373
CspI	CGGWCCG	2 cut(s)	570, 1174
CviAII	CATG	7 cut(s)	1328, 2651, 2887, 3019, 3025, 3113, 3359
CviQI	GTAC	7 cut(s)	1106, 2515, 2732, 3106, 3237, 3344, 3373
DinI	GGCGCC	1 cut(s)	528
DraI	TTTAAA	1 cut(s)	1782
DraIII	CACNNNGTG	1 cut(s)	948
DrdI	GACNNNNNNGTC	1 cut(s)	1129
DriI	GACNNNNNGTC	2 cut(s)	1023, 1851
DseDI	GACNNNNNNGTC	1 cut(s)	1129
EaeI	YGGCCR	3 cut(s)	408, 538, 1870
Eam1104I	CTCTTC	6 cut(s)	47, 798, 804, 813, 1038, 3033
Eam1105I	GACNNNNNGTC	2 cut(s)	1023, 1851
EarI	CTCTTC	6 cut(s)	47, 798, 804, 813, 1038, 3033
EciI	GGCGGA	4 cut(s)	249, 382, 711, 3010
Ecl136II	GAGCTC	1 cut(s)	861
Eco130I	CCWWGG	3 cut(s)	71, 2886, 3112
Eco147I	AGGCCT	1 cut(s)	1888
Eco24I	GRGCYC	3 cut(s)	863, 1050, 1065
Eco31I	GGTCTC	3 cut(s)	1116, 1194, 1948
Eco47I	GGWCC	7 cut(s)	555, 570, 706, 787, 882, 1174, 2414
Eco53kI	GAGCTC	1 cut(s)	861
Eco57I	CTGAAG	3 cut(s)	2399, 2772, 3057
Eco81I	CCTNAGG	1 cut(s)	111
Eco88I	CYCGRG	3 cut(s)	649, 742, 1933
EcoICRI	GAGCTC	1 cut(s)	861
EcoNI	CCTNNNNNAGG	1 cut(s)	3089
EcoO109I	RGGNCCY	1 cut(s)	555
EcoRI	GAATTC	2 cut(s)	1750, 3354
EcoRII	CCWGG	4 cut(s)	603, 884, 3084, 3349
EcoT14I	CCWWGG	3 cut(s)	71, 2886, 3112
EcoT22I	ATGCAT	3 cut(s)	1333, 1414, 2656
EcoT38I	GRGCYC	3 cut(s)	863, 1050, 1065
EgeI	GGCGCC	1 cut(s)	528
EheI	GGCGCC	1 cut(s)	528
ErhI	CCWWGG	3 cut(s)	71, 2886, 3112
Esp3I	CGTCTC	3 cut(s)	786, 1089, 2768
FaeI	CATG	7 cut(s)	1331, 2654, 2890, 3022, 3028, 3116, 3362
FalI	AAGNNNNNCTT	2 cut(s)	2427, 2459
FaqI	GGGAC	7 cut(s)	311, 568, 1011, 1519, 2212, 2644, 3067
FatI	CATG	7 cut(s)	1327, 2650, 2886, 3018, 3024, 3112, 3358
FauI	CCCGC	5 cut(s)	165, 280, 310, 647, 901
FbaI	TGATCA	1 cut(s)	1528
FblI	GTMKAC	2 cut(s)	645, 696
FokI	GGATG	7 cut(s)	139, 229, 485, 491, 2321, 2587, 2632
FriOI	GRGCYC	3 cut(s)	863, 1050, 1065
FspBI	CTAG	4 cut(s)	1857, 1884, 2540, 3126
GlaI	GCGC	3 cut(s)	528, 673, 2256
GsaI	CCCAGC	3 cut(s)	281, 334, 752
GsuI	CTGGAG	3 cut(s)	331, 695, 1875
HaeII	RGCGCY	3 cut(s)	530, 675, 2258
HaeIII	GGCC	9 cut(s)	76, 369, 410, 540, 753, 1511, 1872, 1888, 2829
HgaI	GACGC	1 cut(s)	1093
HhaI	GCGC	3 cut(s)	529, 674, 2257
Hin1I	GRCGYC	3 cut(s)	527, 1085, 1098
Hin1II	CATG	7 cut(s)	1331, 2654, 2890, 3022, 3028, 3116, 3362
Hin6I	GCGC	3 cut(s)	527, 672, 2255
HinP1I	GCGC	3 cut(s)	527, 672, 2255
HincII	GTYRAC	6 cut(s)	646, 697, 897, 1122, 1497, 2686
HindII	GTYRAC	6 cut(s)	646, 697, 897, 1122, 1497, 2686
HindIII	AAGCTT	4 cut(s)	2286, 2408, 2585, 2921
HpaI	GTTAAC	1 cut(s)	1497
Hpy166II	GTNNAC	7 cut(s)	646, 697, 897, 977, 1122, 1497, 2686
Hpy8I	GTNNAC	7 cut(s)	646, 697, 897, 977, 1122, 1497, 2686
Hpy99I	CGWCG	9 cut(s)	401, 404, 438, 470, 949, 1087, 1120, 1198, 1219
HpyCH4IV	ACGT	7 cut(s)	944, 1098, 2371, 2448, 2487, 2688, 2868
HpySE526I	ACGT	7 cut(s)	944, 1098, 2371, 2448, 2487, 2688, 2868
Hsp92I	GRCGYC	3 cut(s)	527, 1085, 1098
Hsp92II	CATG	7 cut(s)	1331, 2654, 2890, 3022, 3028, 3116, 3362
HspAI	GCGC	3 cut(s)	527, 672, 2255
KasI	GGCGCC	1 cut(s)	526
KflI	GGGWCCC	1 cut(s)	555
Kpn2I	TCCGGA	2 cut(s)	567, 1516
Ksp22I	TGATCA	1 cut(s)	1528
KspAI	GTTAAC	1 cut(s)	1497
LguI	GCTCTTC	2 cut(s)	47, 1038
Lsp1109I	GCAGC	9 cut(s)	945, 1135, 1639, 1798, 2427, 2533, 2866, 2869, 3291
LweI	GCATC	8 cut(s)	74, 1178, 1237, 1340, 1399, 2299, 2464, 2635
MaeI	CTAG	4 cut(s)	1857, 1884, 2540, 3126
MaeII	ACGT	7 cut(s)	944, 1098, 2371, 2448, 2487, 2688, 2868
MbiI	CCGCTC	1 cut(s)	2177
MflI	RGATCY	4 cut(s)	497, 738, 875, 1702
MhlI	GDGCHC	6 cut(s)	390, 611, 863, 1050, 1065, 3398
MlsI	TGGCCA	1 cut(s)	1872
MluI	ACGCGT	1 cut(s)	1073
MluNI	TGGCCA	1 cut(s)	1872
Mly113I	GGCGCC	1 cut(s)	527
MmeI	TCCRAC	5 cut(s)	270, 888, 1965, 2079, 2378
Mox20I	TGGCCA	1 cut(s)	1872
Mph1103I	ATGCAT	3 cut(s)	1333, 1414, 2656
MroI	TCCGGA	2 cut(s)	567, 1516
MroXI	GAANNNNTTC	2 cut(s)	2598, 3020
MscI	TGGCCA	1 cut(s)	1872
MseI	TTAA	5 cut(s)	1469, 1496, 1781, 2520, 2691
MslI	CAYNNNNRTG	2 cut(s)	11, 3023
Msp20I	TGGCCA	1 cut(s)	1872
MspA1I	CMGCKG	1 cut(s)	1652
MspR9I	CCNGG	7 cut(s)	605, 880, 886, 1934, 1935, 3086, 3351
Mva1269I	GAATGC	3 cut(s)	419, 769, 2654
MvaI	CCWGG	4 cut(s)	605, 886, 3086, 3351
MvnI	CGCG	3 cut(s)	855, 1075, 2061
NarI	GGCGCC	1 cut(s)	527
NciI	CCSGG	3 cut(s)	880, 1934, 1935
NcoI	CCATGG	2 cut(s)	2886, 3112
NlaIII	CATG	7 cut(s)	1331, 2654, 2890, 3022, 3028, 3116, 3362
NmeAIII	GCCGAG	3 cut(s)	345, 516, 712
NmuCI	GTSAC	6 cut(s)	1015, 1377, 1563, 1623, 2263, 2488
NruI	TCGCGA	1 cut(s)	855
NsiI	ATGCAT	3 cut(s)	1333, 1414, 2656
NspI	RCATGY	2 cut(s)	1331, 3022
PaeR7I	CTCGAG	1 cut(s)	742
PagI	TCATGA	2 cut(s)	3024, 3358
PceI	AGGCCT	1 cut(s)	1888
PciI	ACATGT	1 cut(s)	3018
PciSI	GCTCTTC	2 cut(s)	47, 1038
PcsI	WCGNNNNNNNCGW	6 cut(s)	408, 1079, 1202, 1211, 1373, 3381
PctI	GAATGC	3 cut(s)	419, 769, 2654
PdmI	GAANNNNTTC	2 cut(s)	2598, 3020
PfeI	GAWTC	7 cut(s)	116, 1398, 1577, 1592, 1924, 2152, 3150
PfoI	TCCNGGA	2 cut(s)	884, 3349
Ple19I	CGATCG	1 cut(s)	853
PluTI	GGCGCC	1 cut(s)	530
Ppu21I	YACGTR	1 cut(s)	2488
PpuMI	RGGWCCY	1 cut(s)	555
PscI	ACATGT	1 cut(s)	3018
PsiI	TTATAA	1 cut(s)	3050
Psp124BI	GAGCTC	1 cut(s)	863
Psp5II	RGGWCCY	1 cut(s)	555
Psp6I	CCWGG	4 cut(s)	603, 884, 3084, 3349
PspFI	CCCAGC	3 cut(s)	277, 330, 748
PspGI	CCWGG	4 cut(s)	603, 884, 3084, 3349
PspPI	GGNCC	8 cut(s)	555, 570, 706, 751, 787, 882, 1174, 2414
PspPPI	RGGWCCY	1 cut(s)	555
PsuI	RGATCY	4 cut(s)	497, 738, 875, 1702
PvuI	CGATCG	1 cut(s)	853
PvuII	CAGCTG	1 cut(s)	1652
RgaI	GCGATCGC	1 cut(s)	853
RruI	TCGCGA	1 cut(s)	855
RsaI	GTAC	7 cut(s)	1107, 2516, 2733, 3107, 3238, 3345, 3374
RsaNI	GTAC	7 cut(s)	1106, 2515, 2732, 3106, 3237, 3344, 3373
RseI	CAYNNNNRTG	2 cut(s)	11, 3023
Rsr2I	CGGWCCG	2 cut(s)	570, 1174
RsrII	CGGWCCG	2 cut(s)	570, 1174
SacI	GAGCTC	1 cut(s)	863
SalI	GTCGAC	2 cut(s)	644, 695
SapI	GCTCTTC	2 cut(s)	47, 1038
SaqAI	TTAA	5 cut(s)	1469, 1496, 1781, 2520, 2691
Sau96I	GGNCC	8 cut(s)	555, 570, 706, 751, 787, 882, 1174, 2414
ScaI	AGTACT	2 cut(s)	3107, 3345
ScrFI	CCNGG	7 cut(s)	605, 880, 886, 1934, 1935, 3086, 3351
SduI	GDGCHC	6 cut(s)	390, 611, 863, 1050, 1065, 3398
SfaAI	GCGATCGC	1 cut(s)	853
SfaNI	GCATC	8 cut(s)	74, 1178, 1237, 1340, 1399, 2299, 2464, 2635
SfcI	CTRYAG	2 cut(s)	82, 547
SfoI	GGCGCC	1 cut(s)	528
Sfr274I	CTCGAG	1 cut(s)	742
SgfI	GCGATCGC	1 cut(s)	853
SinI	GGWCC	7 cut(s)	555, 570, 706, 787, 882, 1174, 2414
SlaI	CTCGAG	1 cut(s)	742
SmaI	CCCGGG	1 cut(s)	1935
SmiMI	CAYNNNNRTG	2 cut(s)	11, 3023
SmlI	CTYRAG	4 cut(s)	632, 742, 2675, 3063
SmoI	CTYRAG	4 cut(s)	632, 742, 2675, 3063
SseBI	AGGCCT	1 cut(s)	1888
SspDI	GGCGCC	1 cut(s)	526
SspI	AATATT	1 cut(s)	2236
SspMI	CTAG	4 cut(s)	1857, 1884, 2540, 3126
SstI	GAGCTC	1 cut(s)	863
StuI	AGGCCT	1 cut(s)	1888
StyD4I	CCNGG	7 cut(s)	603, 878, 884, 1932, 1933, 3084, 3349
StyI	CCWWGG	3 cut(s)	71, 2886, 3112
TaiI	ACGT	7 cut(s)	947, 1101, 2374, 2451, 2490, 2691, 2871
TaqII	GACCGA	3 cut(s)	804, 939, 1191
TatI	WGTACW	2 cut(s)	3105, 3343
TauI	GCSGC	7 cut(s)	130, 133, 383, 419, 456, 718, 727
TfiI	GAWTC	7 cut(s)	116, 1398, 1577, 1592, 1924, 2152, 3150
Tru1I	TTAA	5 cut(s)	1469, 1496, 1781, 2520, 2691
Tru9I	TTAA	5 cut(s)	1469, 1496, 1781, 2520, 2691
TscAI	CASTG	9 cut(s)	67, 556, 1134, 1836, 2196, 2409, 2773, 2914, 3084
TseFI	GTSAC	6 cut(s)	1015, 1377, 1563, 1623, 2263, 2488
TseI	GCWGC	9 cut(s)	933, 1148, 1652, 1786, 2440, 2546, 2879, 2882, 3304
Tsp45I	GTSAC	6 cut(s)	1015, 1377, 1563, 1623, 2263, 2488
TspGWI	ACGGA	7 cut(s)	882, 888, 1104, 1182, 1197, 1370, 3393
TspMI	CCCGGG	1 cut(s)	1933
TspRI	CASTG	9 cut(s)	67, 556, 1134, 1836, 2196, 2409, 2773, 2914, 3084
VpaK11BI	GGWCC	7 cut(s)	555, 570, 706, 787, 882, 1174, 2414
XagI	CCTNNNNNAGG	1 cut(s)	3089
XapI	RAATTY	7 cut(s)	1471, 1665, 1750, 2781, 2999, 3248, 3354
XbaI	TCTAGA	1 cut(s)	2539
XceI	RCATGY	2 cut(s)	1331, 3022
XcmI	CCANNNNNNNNNTGG	1 cut(s)	548
XhoI	CTCGAG	1 cut(s)	742
XmaI	CCCGGG	1 cut(s)	1933
XmiI	GTMKAC	2 cut(s)	645, 696
XmnI	GAANNNNTTC	2 cut(s)	2598, 3020
XspI	CTAG	4 cut(s)	1857, 1884, 2540, 3126
ZraI	GACGTC	1 cut(s)	1099
ZrmI	AGTACT	2 cut(s)	3107, 3345
Zsp2I	ATGCAT	3 cut(s)	1333, 1414, 2656

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.