pycom10g12820

FtsJ-like methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009475
Physical Location & Seq
Reverse (-)
16312146 .. 16313456
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g12820.1

Sequence Viewer

Length: 393 bp
ATGGGGCTTCAGCTTCTCAAGCTCCCAAATCACACCGTAACCTCCTGCCGTTTTGCAAGAACGAGTTCAAGCTTCTCTTTTCTCTTCTCGCAACCCAACTTACAGGAGCTCCCTGCGAGATGGGTCGGAGTGCCAATGCATAGAACCTTTGCTTCTGTGAAGTTTGCAAAGGCTCAACCACCAAAGAAGAGGAAGAGGCTTGATGAGGTATGCCTCGAAAGGTTTCAACAATATAGTCGAACCTTCATCCAGTCGTGGATCTTACAAGGGAAAGTATTTGTGGATGGAAAGGTAGTCAGCAAAGCTGGGACTCCAGTCTCGGACAAAGCTGTTGTACAGATAATGGCTGAAGTCCCAAAATATAGCAGGACACAAGTTAGAAGCTGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ S4

Protein Analysis

131

Amino Acids

14.83

Weight (kDa)

10.49

Isoelectric Point (pI)

63.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S4 PF01479 66 - 111 1.4e-09 S4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 266
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 1 cut(s) 336
AgsI TTSAA 2 cut(s) 69, 227
AluBI AGCT 7 cut(s) 13, 22, 72, 109, 305, 329, 384
AluI AGCT 7 cut(s) 13, 22, 72, 109, 305, 329, 384
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 1 cut(s) 266
ApeKI GCWGC 1 cut(s) 384
Asp700I GAANNNNTTC 2 cut(s) 64, 222
BanII GRGCYC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 1 cut(s) 371
BccI CCATC 2 cut(s) 114, 278
BceAI ACGGC 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 322
BisI GCNGC 1 cut(s) 385
BlsI GCNGC 1 cut(s) 386
BoxI GACNNNNGTC 1 cut(s) 314
BpmI CTGGAG 1 cut(s) 297
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bse1I ACTGG 2 cut(s) 250, 314
BseGI GGATG 2 cut(s) 246, 289
BseNI ACTGG 2 cut(s) 250, 314
BseXI GCAGC 1 cut(s) 371
BseYI CCCAGC 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 111
BslFI GGGAC 2 cut(s) 322, 338
BsmAI GTCTC 1 cut(s) 322
BsmFI GGGAC 2 cut(s) 322, 338
Bsp1286I GDGCHC 1 cut(s) 111
Bsp1407I TGTACA 1 cut(s) 334
Bsp143I GATC 1 cut(s) 258
BspPI GGATC 1 cut(s) 266
BsrGI TGTACA 1 cut(s) 334
BsrI ACTGG 2 cut(s) 250, 314
BssMI GATC 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 3 cut(s) 89, 182, 188
BstAUI TGTACA 1 cut(s) 334
BstF5I GGATG 2 cut(s) 246, 289
BstKTI GATC 1 cut(s) 261
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 1 cut(s) 258
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstPAI GACNNNNGTC 1 cut(s) 314
BstV1I GCAGC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
BtsCI GGATG 2 cut(s) 246, 289
Csp6I GTAC 1 cut(s) 335
CviQI GTAC 1 cut(s) 335
DpnI GATC 1 cut(s) 260
DpnII GATC 1 cut(s) 258
Eam1104I CTCTTC 3 cut(s) 89, 182, 188
EarI CTCTTC 3 cut(s) 89, 182, 188
Ecl136II GAGCTC 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 111
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 369
EcoICRI GAGCTC 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 111
FaiI YATR 4 cut(s) 141, 211, 234, 363
FalI AAGNNNNNCTT 2 cut(s) 61, 93
FaqI GGGAC 2 cut(s) 322, 338
Fnu4HI GCNGC 1 cut(s) 385
FokI GGATG 2 cut(s) 233, 296
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 385
GluI GCNGC 1 cut(s) 385
GsaI CCCAGC 1 cut(s) 309
GsuI CTGGAG 1 cut(s) 297
HindIII AAGCTT 1 cut(s) 70
HinfI GANTC 1 cut(s) 310
Hpy188I TCNGA 2 cut(s) 128, 322
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 3 cut(s) 56, 139, 167
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
Kzo9I GATC 1 cut(s) 258
LmnI GCTCC 3 cut(s) 27, 106, 114
LpnPI CCDG 7 cut(s) 58, 89, 126, 263, 291, 327, 352
Lsp1109I GCAGC 1 cut(s) 371
MaeIII GTNAC 1 cut(s) 37
MalI GATC 1 cut(s) 260
MboI GATC 1 cut(s) 258
MboII GAAGA 3 cut(s) 76, 199, 205
MflI RGATCY 1 cut(s) 258
MhlI GDGCHC 1 cut(s) 111
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 1 cut(s) 106
MnlI CCTC 5 cut(s) 52, 183, 189, 199, 224
Mph1103I ATGCAT 1 cut(s) 141
MroXI GAANNNNTTC 2 cut(s) 64, 222
MwoI GCNNNNNNNGC 1 cut(s) 19
NdeII GATC 1 cut(s) 258
NsiI ATGCAT 1 cut(s) 141
PdmI GAANNNNTTC 2 cut(s) 64, 222
PkrI GCNGC 1 cut(s) 386
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PshAI GACNNNNGTC 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 111
PspFI CCCAGC 1 cut(s) 305
PsuI RGATCY 1 cut(s) 258
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
SacI GAGCTC 1 cut(s) 111
SatI GCNGC 1 cut(s) 385
Sau3AI GATC 1 cut(s) 258
SchI GAGTC 1 cut(s) 304
SduI GDGCHC 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
SstI GAGCTC 1 cut(s) 111
TaaI ACNGT 1 cut(s) 37
TaqI TCGA 2 cut(s) 216, 238
TatI WGTACW 1 cut(s) 334
TseI GCWGC 1 cut(s) 384
TspDTI ATGAA 1 cut(s) 235
XmnI GAANNNNTTC 2 cut(s) 64, 222
Zsp2I ATGCAT 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.