Rorug06G0146400

FtsJ-like methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009475
Physical Location & Seq
Forward (+)
20916754 .. 20919624
2871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0146400.1

Sequence Viewer

Length: 1041 bp
ATGCACTCCGTCCAGAGATGCCGGCCAAGGCCTCCGAGAAGGAGATCAAAGAACCTTGAGCTCTTCTTGAAGGTCGACGGACTGGATGAGAGGACGGCCAAACACACAATCGCCAACAAAAACGTCACCGCCGTCATTCACGAGGCTGCAGTTACTCATGGATGCAGTCGAAGCATTGGTAATCAATCTTCTATACACGGTTGCTATAAAGTGTCCAGCAAATGCCCTTGTGCATCGCCCGTATTGGCACACTACATTGTGTCTTCAAAGGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATATCTGTTAATGAGGTTTTTGAAGAGAATAAGTCTGCAATTTTGGAGCAGCGCTATAAAACAAATGCGGGTGACTTATTTGCACATCTTAGGAAGAGACATCCGTGGGCTGACCCAGAAGTTGTCAAGGCAATCTTATTTTACTCTTTTGAATGAAATTGCTATTGCTATTTCTACCCTGAATAGCCAGTTGAAGAAATTCTTAATCCATATGCGATATTCCCTCAACCAGAGGAAAACTTTAAGAGACTTTGCTATATATTCTGGTGTAAATTTTCAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCCATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAATCACTTGAGTGTGACTGGGGGGAGAGTTTTAACTCGCTTTCCTCCTGAGCTGAATGGATATATACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGAAAAAGAGAGAGGTGGTGGCTGCTATCTGAGGTTTGATGACATAAATCACGAAGCTGAAAAGAAAGAATATATTGATCATATTCAGGAAATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTACGTTGATCATCAGACGGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAAATGAACAGCCCTTGGAGGGGCAGGCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ S4

Protein Analysis

346

Amino Acids

39.96

Weight (kDa)

9.11

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c PF00749 228 - 338 5.7e-43 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 75
AciI CCGC 2 cut(s) 129, 373
AcoI YGGCCR 2 cut(s) 23, 96
AcsI RAATTY 2 cut(s) 503, 577
AcuI CTGAAG 1 cut(s) 579
AfaI GTAC 1 cut(s) 995
AfeI AGCGCT 1 cut(s) 358
AfiI CCNNNNNNNGG 3 cut(s) 736, 1025, 1026
AgsI TTSAA 7 cut(s) 70, 267, 281, 328, 457, 499, 653
AhlI ACTAGT 1 cut(s) 896
AjnI CCWGG 1 cut(s) 759
AleI CACNNNNGTG 1 cut(s) 663
AluBI AGCT 4 cut(s) 61, 706, 818, 937
AluI AGCT 4 cut(s) 61, 706, 818, 937
Alw21I GWGCWC 2 cut(s) 63, 939
Alw26I GTCTC 2 cut(s) 396, 546
Aor51HI AGCGCT 1 cut(s) 358
AoxI GGCC 6 cut(s) 23, 29, 96, 727, 757, 1032
ApeKI GCWGC 3 cut(s) 146, 354, 783
ApoI RAATTY 2 cut(s) 503, 577
Asp700I GAANNNNTTC 1 cut(s) 503
AspLEI GCGC 1 cut(s) 359
AsuHPI GGTGA 2 cut(s) 118, 388
BanII GRGCYC 3 cut(s) 63, 879, 939
BarI GAAGNNNNNNTAC 2 cut(s) 172, 204
BauI CACGAG 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 2 cut(s) 63, 939
BbvI GCAGC 3 cut(s) 133, 366, 770
BccI CCATC 2 cut(s) 605, 859
BceAI ACGGC 2 cut(s) 111, 116
BciT130I CCWGG 1 cut(s) 761
BclI TGATCA 2 cut(s) 838, 964
BcoDI GTCTC 2 cut(s) 396, 546
BcuI ACTAGT 1 cut(s) 896
BfaI CTAG 1 cut(s) 897
BfmI CTRYAG 1 cut(s) 147
BfoI RGCGCY 1 cut(s) 360
BglII AGATCT 1 cut(s) 913
BisI GCNGC 3 cut(s) 147, 355, 784
BlsI GCNGC 3 cut(s) 148, 356, 785
Bme1390I CCNGG 1 cut(s) 761
BmiI GGNNCC 2 cut(s) 616, 876
BmrFI CCNGG 1 cut(s) 761
BmrI ACTGGG 1 cut(s) 681
BmsI GCATC 3 cut(s) 8, 152, 242
BmuI ACTGGG 1 cut(s) 681
BpiI GAAGAC 1 cut(s) 255
Bpu10I CCTNAGC 1 cut(s) 702
BpuEI CTTGAG 2 cut(s) 77, 682
BsaJI CCNNGG 4 cut(s) 26, 409, 735, 1020
Bsc4I CCNNNNNNNGG 3 cut(s) 736, 1025, 1026
Bse118I RCCGGY 1 cut(s) 21
Bse1I ACTGG 3 cut(s) 87, 493, 676
Bse3DI GCAATG 1 cut(s) 747
BseBI CCWGG 1 cut(s) 761
BseDI CCNNGG 4 cut(s) 26, 409, 735, 1020
BseGI GGATG 5 cut(s) 91, 167, 405, 870, 985
BseLI CCNNNNNNNGG 3 cut(s) 736, 1025, 1026
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 3 cut(s) 614, 693, 782
BseNI ACTGG 3 cut(s) 87, 493, 676
BseXI GCAGC 3 cut(s) 133, 366, 770
BshFI GGCC 6 cut(s) 25, 31, 98, 729, 759, 1034
BsiHKAI GWGCWC 2 cut(s) 63, 939
BsiSI CCGG 1 cut(s) 22
BslI CCNNNNNNNGG 3 cut(s) 736, 1025, 1026
BsmAI GTCTC 2 cut(s) 396, 546
BsnI GGCC 6 cut(s) 25, 31, 98, 729, 759, 1034
Bsp1286I GDGCHC 3 cut(s) 63, 879, 939
Bsp143I GATC 5 cut(s) 44, 838, 885, 913, 964
BspACI CCGC 2 cut(s) 129, 373
BspANI GGCC 6 cut(s) 25, 31, 98, 729, 759, 1034
BspCNI CTCAG 3 cut(s) 615, 694, 783
BspLI GGNNCC 2 cut(s) 616, 876
BspMAI CTGCAG 1 cut(s) 151
BspQI GCTCTTC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 747
BsrFI RCCGGY 1 cut(s) 21
BsrI ACTGG 3 cut(s) 87, 493, 676
BssAI RCCGGY 1 cut(s) 21
BssECI CCNNGG 4 cut(s) 26, 409, 735, 1020
BssMI GATC 5 cut(s) 44, 838, 885, 913, 964
BssSI CACGAG 1 cut(s) 140
BssT1I CCWWGG 3 cut(s) 26, 735, 1020
Bst2BI CACGAG 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 761
Bst4CI ACNGT 1 cut(s) 200
Bst6I CTCTTC 3 cut(s) 68, 323, 394
BstC8I GCNNGC 2 cut(s) 23, 1032
BstDEI CTNAG 4 cut(s) 394, 623, 702, 791
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 5 cut(s) 91, 167, 405, 870, 985
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 5 cut(s) 47, 841, 888, 916, 967
BstMAI GTCTC 2 cut(s) 396, 546
BstMBI GATC 5 cut(s) 44, 838, 885, 913, 964
BstMWI GCNNNNNNNGC 1 cut(s) 171
BstNI CCWGG 1 cut(s) 761
BstSCI CCNGG 1 cut(s) 759
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 133, 366, 770
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 1 cut(s) 913
BstYI RGATCY 1 cut(s) 913
BsuRI GGCC 6 cut(s) 25, 31, 98, 729, 759, 1034
BtgI CCRYGG 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 219
BtsCI GGATG 5 cut(s) 91, 167, 405, 870, 985
BtsI GCAGTG 1 cut(s) 936
BtsIMutI CAGTG 1 cut(s) 936
Cac8I GCNNGC 2 cut(s) 23, 1032
CfoI GCGC 1 cut(s) 359
Cfr10I RCCGGY 1 cut(s) 21
Csp6I GTAC 1 cut(s) 994
CviAII CATG 2 cut(s) 158, 731
CviQI GTAC 1 cut(s) 994
DdeI CTNAG 4 cut(s) 394, 623, 702, 791
DpnI GATC 5 cut(s) 46, 840, 887, 915, 966
DpnII GATC 5 cut(s) 44, 838, 885, 913, 964
EaeI YGGCCR 2 cut(s) 23, 96
Eam1104I CTCTTC 3 cut(s) 68, 323, 394
EarI CTCTTC 3 cut(s) 68, 323, 394
Ecl136II GAGCTC 2 cut(s) 61, 937
Eco130I CCWWGG 3 cut(s) 26, 735, 1020
Eco147I AGGCCT 1 cut(s) 31
Eco24I GRGCYC 3 cut(s) 63, 879, 939
Eco47III AGCGCT 1 cut(s) 358
Eco53kI GAGCTC 2 cut(s) 61, 937
Eco57I CTGAAG 1 cut(s) 579
EcoICRI GAGCTC 2 cut(s) 61, 937
EcoRII CCWGG 1 cut(s) 759
EcoT14I CCWWGG 3 cut(s) 26, 735, 1020
EcoT38I GRGCYC 3 cut(s) 63, 879, 939
ErhI CCWWGG 3 cut(s) 26, 735, 1020
FaeI CATG 2 cut(s) 161, 734
FalI AAGNNNNNCTT 4 cut(s) 424, 456, 491, 523
FatI CATG 2 cut(s) 157, 730
FauI CCCGC 1 cut(s) 366
FauNDI CATATG 1 cut(s) 516
FbaI TGATCA 2 cut(s) 838, 964
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 3 cut(s) 147, 355, 784
FokI GGATG 5 cut(s) 98, 174, 392, 877, 992
FriOI GRGCYC 3 cut(s) 63, 879, 939
Fsp4HI GCNGC 3 cut(s) 147, 355, 784
FspBI CTAG 1 cut(s) 897
GlaI GCGC 1 cut(s) 358
GluI GCNGC 3 cut(s) 147, 355, 784
HaeII RGCGCY 1 cut(s) 360
HaeIII GGCC 6 cut(s) 25, 31, 98, 729, 759, 1034
HapII CCGG 1 cut(s) 22
HhaI GCGC 1 cut(s) 359
Hin1II CATG 2 cut(s) 161, 734
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HpaII CCGG 1 cut(s) 22
HphI GGTGA 2 cut(s) 118, 388
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 5 cut(s) 36, 289, 624, 792, 972
Hpy188III TCNNGA 6 cut(s) 13, 67, 140, 701, 812, 848
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 3 cut(s) 33, 64, 939
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4IV ACGT 2 cut(s) 123, 960
HpyCH4V TGCA 7 cut(s) 4, 149, 165, 233, 343, 388, 633
HpyF10VI GCNNNNNNNGC 1 cut(s) 171
HpyF3I CTNAG 4 cut(s) 394, 623, 702, 791
HpySE526I ACGT 2 cut(s) 123, 960
Hsp92II CATG 2 cut(s) 161, 734
HspAI GCGC 1 cut(s) 357
KroI GCCGGC 1 cut(s) 21
KroNI GCCGGC 1 cut(s) 23
Ksp22I TGATCA 2 cut(s) 838, 964
Kzo9I GATC 5 cut(s) 44, 838, 885, 913, 964
LguI GCTCTTC 1 cut(s) 68
LmnI GCTCC 3 cut(s) 351, 874, 934
Lsp1109I GCAGC 3 cut(s) 133, 366, 770
LweI GCATC 3 cut(s) 8, 152, 242
MaeI CTAG 1 cut(s) 897
MaeII ACGT 2 cut(s) 123, 960
MaeIII GTNAC 5 cut(s) 124, 151, 376, 667, 950
MalI GATC 5 cut(s) 46, 840, 887, 915, 966
MboI GATC 5 cut(s) 44, 838, 885, 913, 964
MboII GAAGA 8 cut(s) 55, 180, 255, 302, 305, 340, 411, 511
MfeI CAATTG 1 cut(s) 859
MflI RGATCY 1 cut(s) 913
MhlI GDGCHC 3 cut(s) 63, 879, 939
MluCI AATT 8 cut(s) 344, 462, 503, 577, 852, 859, 923, 1036
MroNI GCCGGC 1 cut(s) 21
MroXI GAANNNNTTC 1 cut(s) 503
MseI TTAA 5 cut(s) 314, 509, 548, 686, 1039
MslI CAYNNNNRTG 1 cut(s) 663
MspI CCGG 1 cut(s) 22
MspR9I CCNGG 1 cut(s) 761
MunI CAATTG 1 cut(s) 859
MvaI CCWGG 1 cut(s) 761
MwoI GCNNNNNNNGC 1 cut(s) 171
NaeI GCCGGC 1 cut(s) 23
NdeI CATATG 1 cut(s) 516
NdeII GATC 5 cut(s) 44, 838, 885, 913, 964
NgoMIV GCCGGC 1 cut(s) 21
NlaIII CATG 2 cut(s) 161, 734
NlaIV GGNNCC 2 cut(s) 616, 876
NmuCI GTSAC 4 cut(s) 124, 376, 667, 950
OliI CACNNNNGTG 1 cut(s) 663
PceI AGGCCT 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 68
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PdiI GCCGGC 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 503
PkrI GCNGC 3 cut(s) 148, 356, 785
Psp124BI GAGCTC 2 cut(s) 63, 939
Psp6I CCWGG 1 cut(s) 759
PspGI CCWGG 1 cut(s) 759
PspN4I GGNNCC 2 cut(s) 616, 876
PstI CTGCAG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 913
RsaI GTAC 1 cut(s) 995
RsaNI GTAC 1 cut(s) 994
RseI CAYNNNNRTG 1 cut(s) 663
SacI GAGCTC 2 cut(s) 63, 939
SalI GTCGAC 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 68
SaqAI TTAA 5 cut(s) 314, 509, 548, 686, 1039
SatI GCNGC 3 cut(s) 147, 355, 784
Sau3AI GATC 5 cut(s) 44, 838, 885, 913, 964
ScrFI CCNGG 1 cut(s) 761
SduI GDGCHC 3 cut(s) 63, 879, 939
SfaNI GCATC 3 cut(s) 8, 152, 242
SfcI CTRYAG 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 2 cut(s) 56, 661
SmoI CTYRAG 2 cut(s) 56, 661
SpeI ACTAGT 1 cut(s) 896
Sse9I AATT 8 cut(s) 344, 462, 503, 577, 852, 859, 923, 1036
SseBI AGGCCT 1 cut(s) 31
SsiI CCGC 2 cut(s) 129, 373
SspMI CTAG 1 cut(s) 897
SstI GAGCTC 2 cut(s) 63, 939
StuI AGGCCT 1 cut(s) 31
StyD4I CCNGG 1 cut(s) 759
StyI CCWWGG 3 cut(s) 26, 735, 1020
TaaI ACNGT 1 cut(s) 200
TaiI ACGT 2 cut(s) 126, 963
TaqI TCGA 2 cut(s) 75, 169
TasI AATT 8 cut(s) 344, 462, 503, 577, 852, 859, 923, 1036
TatI WGTACW 1 cut(s) 993
Tru1I TTAA 5 cut(s) 314, 509, 548, 686, 1039
Tru9I TTAA 5 cut(s) 314, 509, 548, 686, 1039
TscAI CASTG 1 cut(s) 936
TseFI GTSAC 4 cut(s) 124, 376, 667, 950
TseI GCWGC 3 cut(s) 146, 354, 783
Tsp45I GTSAC 4 cut(s) 124, 376, 667, 950
TspDTI ATGAA 4 cut(s) 474, 578, 936, 1026
TspGWI ACGGA 2 cut(s) 93, 398
TspRI CASTG 1 cut(s) 936
XapI RAATTY 2 cut(s) 503, 577
XmiI GTMKAC 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 503
XspI CTAG 1 cut(s) 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.