pycom10g15040

rpm1 interacting protein 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008119
Physical Location & Seq
Reverse (-)
18367515 .. 18369286
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g15040.1

Sequence Viewer

Length: 843 bp
ATGGCATCCCCCATATCGACTCCTGGATTCCCTTCTGTGCGCGCCCGCCAATCCGCTGCCACTTGTGACTCTGCCATGGAAACTCGCGTTGCTTCCTTGGAGGCTATCATCGCCTCTTTACCTTCCCTCATCGCCACTGCGATCGACAATGCCCTTGAAAAGGCCTTCGAAAAGGCCTTCGATGCCTCTCTTGAAAAATTTCGTCGGGAAATGCTCTCTCGAGGTGCCGGGGAAGGCATCTCTGCCCCTCTTGCCGGCGATCATCATGCTTCTCCTGCAATTGATCCGATCCAGCTACCTCGGAGCCCAATCTGTGCTGAAGGAGCCCGATCGCATCTCTCCGACGACCAACTGCCTCGGATCGACCTGGATGAGGAGGACAACTTTGATTGTGTATCGGAGCTTCTGGAATCCGTCGACAAGGTAACCGACGACGATGATTCCGATGACATTGTGGTGATCAGTGAGGTCAACTCAAAGCCTAGCCAAAAGTCTTCGAAACGGACGGTGGCGGATGATGACGATGATTGTGTGGTCTTGGACGGCGACCCGGATAAGCCGGTTTCGGTGGTGGAGGATTCGGGTAGCGGCTCCGACGAGTTGCTTGTAGTTGGGGAAACGGGGCAGGTAGCTTGTAGAGACTACCCTCATCCACGACATCTTTGTGCCAAATTCCCTTTCAAAACTACCCGGCCCCAGAACCACTGTAATCTTTGTCATTGTTATGTCTGTGACTCGCTGGCACCATGTGTACATTGGACCTTTAATCATTGTTATGCCACGGATGAAGAAGAGATATATAGAACATGGAGAAAAATATTCAAGACACGGAAAGTTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

30.62

Weight (kDa)

4.71

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 616
AccB1I GGYRCC 2 cut(s) 224, 742
AccI GTMKAC 1 cut(s) 417
AccII CGCG 2 cut(s) 42, 87
AciI CCGC 4 cut(s) 46, 54, 512, 588
AclWI GGATC 3 cut(s) 278, 283, 368
AcsI RAATTY 2 cut(s) 197, 671
AcuI CTGAAG 1 cut(s) 339
AdeI CACNNNGTG 1 cut(s) 749
AfaI GTAC 1 cut(s) 753
AfiI CCNNNNNNNGG 3 cut(s) 160, 254, 373
AgsI TTSAA 4 cut(s) 158, 194, 682, 823
AjnI CCWGG 2 cut(s) 22, 366
AjuI GAANNNNNNNTTGG 2 cut(s) 662, 694
AluBI AGCT 3 cut(s) 295, 403, 632
AluI AGCT 3 cut(s) 295, 403, 632
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 3 cut(s) 278, 283, 368
Ama87I CYCGRG 1 cut(s) 219
AoxI GGCC 3 cut(s) 162, 174, 692
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 2 cut(s) 197, 671
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 2 cut(s) 42, 44
AspS9I GGNCC 2 cut(s) 693, 759
AsuC2I CCSGG 3 cut(s) 229, 551, 691
AsuHPI GGTGA 1 cut(s) 469
AsuII TTCGAA 2 cut(s) 168, 497
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 1 cut(s) 759
BanI GGYRCC 2 cut(s) 224, 742
BanII GRGCYC 2 cut(s) 308, 328
BarI GAAGNNNNNNTAC 2 cut(s) 387, 419
BbsI GAAGAC 1 cut(s) 486
BbvI GCAGC 1 cut(s) 43
BceAI ACGGC 1 cut(s) 559
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 2 cut(s) 24, 368
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 3 cut(s) 229, 551, 691
BcoDI GTCTC 1 cut(s) 633
BfaI CTAG 1 cut(s) 483
BfuAI ACCTGC 1 cut(s) 616
BisI GCNGC 2 cut(s) 57, 589
BlsI GCNGC 2 cut(s) 58, 590
Bme1390I CCNGG 5 cut(s) 24, 229, 368, 551, 691
Bme18I GGWCC 1 cut(s) 759
BmeT110I CYCGRG 1 cut(s) 219
BmgT120I GGNCC 2 cut(s) 693, 759
BmiI GGNNCC 6 cut(s) 226, 305, 325, 592, 695, 744
BmrFI CCNGG 5 cut(s) 24, 229, 368, 551, 691
BmsI GCATC 4 cut(s) 14, 172, 246, 343
BpiI GAAGAC 1 cut(s) 486
BplI GAGNNNNNCTC 2 cut(s) 458, 490
Bpu14I TTCGAA 2 cut(s) 168, 497
BpuMI CCSGG 3 cut(s) 229, 551, 691
BsaJI CCNNGG 6 cut(s) 75, 96, 228, 299, 356, 780
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 254, 373
Bse118I RCCGGY 2 cut(s) 254, 559
BseBI CCWGG 2 cut(s) 24, 368
BseDI CCNNGG 6 cut(s) 75, 96, 228, 299, 356, 780
BseGI GGATG 5 cut(s) 5, 376, 520, 649, 790
BseLI CCNNNNNNNGG 3 cut(s) 160, 254, 373
BsePI GCGCGC 1 cut(s) 40
BseRI GAGGAG 1 cut(s) 389
BseXI GCAGC 1 cut(s) 43
Bsh1236I CGCG 2 cut(s) 42, 87
Bsh1285I CGRYCG 2 cut(s) 144, 332
BshFI GGCC 3 cut(s) 164, 176, 694
BshNI GGYRCC 2 cut(s) 224, 742
BsiEI CGRYCG 2 cut(s) 144, 332
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 5 cut(s) 228, 255, 551, 560, 691
BslI CCNNNNNNNGG 3 cut(s) 160, 254, 373
BsmAI GTCTC 1 cut(s) 633
BsnI GGCC 3 cut(s) 164, 176, 694
BsoBI CYCGRG 1 cut(s) 219
Bsp119I TTCGAA 2 cut(s) 168, 497
Bsp1286I GDGCHC 2 cut(s) 308, 328
Bsp1407I TGTACA 1 cut(s) 751
Bsp143I GATC 7 cut(s) 141, 259, 283, 288, 329, 360, 459
Bsp19I CCATGG 1 cut(s) 75
BspACI CCGC 4 cut(s) 46, 54, 512, 588
BspANI GGCC 3 cut(s) 164, 176, 694
BspFNI CGCG 2 cut(s) 42, 87
BspLI GGNNCC 6 cut(s) 226, 305, 325, 592, 695, 744
BspMI ACCTGC 1 cut(s) 616
BspPI GGATC 3 cut(s) 278, 283, 368
BspT104I TTCGAA 2 cut(s) 168, 497
BspT107I GGYRCC 2 cut(s) 224, 742
BsrFI RCCGGY 2 cut(s) 254, 559
BsrGI TGTACA 1 cut(s) 751
BssAI RCCGGY 2 cut(s) 254, 559
BssECI CCNNGG 6 cut(s) 75, 96, 228, 299, 356, 780
BssHII GCGCGC 1 cut(s) 40
BssMI GATC 7 cut(s) 141, 259, 283, 288, 329, 360, 459
BssT1I CCWWGG 2 cut(s) 75, 96
Bst2UI CCWGG 2 cut(s) 24, 368
Bst4CI ACNGT 2 cut(s) 508, 707
Bst6I CTCTTC 1 cut(s) 786
BstAUI TGTACA 1 cut(s) 751
BstBI TTCGAA 2 cut(s) 168, 497
BstC8I GCNNGC 4 cut(s) 42, 46, 256, 741
BstDSI CCRYGG 2 cut(s) 75, 780
BstEII GGTNACC 1 cut(s) 424
BstENI CCTNNNNNAGG 2 cut(s) 158, 371
BstF5I GGATG 5 cut(s) 5, 376, 520, 649, 790
BstFNI CGCG 2 cut(s) 42, 87
BstHHI GCGC 2 cut(s) 42, 44
BstKTI GATC 7 cut(s) 144, 262, 286, 291, 332, 363, 462
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 7 cut(s) 141, 259, 283, 288, 329, 360, 459
BstMCI CGRYCG 2 cut(s) 144, 332
BstMWI GCNNNNNNNGC 5 cut(s) 110, 182, 251, 275, 323
BstNI CCWGG 2 cut(s) 24, 368
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 5 cut(s) 22, 227, 366, 549, 689
BstUI CGCG 2 cut(s) 42, 87
BstV1I GCAGC 1 cut(s) 43
BstV2I GAAGAC 1 cut(s) 486
BsuRI GGCC 3 cut(s) 164, 176, 694
BtgI CCRYGG 2 cut(s) 75, 780
BtgZI GCGATG 2 cut(s) 94, 115
BtsCI GGATG 5 cut(s) 5, 376, 520, 649, 790
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 3 cut(s) 135, 469, 703
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 4 cut(s) 42, 46, 256, 741
CfoI GCGC 2 cut(s) 42, 44
Cfr10I RCCGGY 2 cut(s) 254, 559
Cfr13I GGNCC 2 cut(s) 693, 759
Csp6I GTAC 1 cut(s) 752
CviAII CATG 4 cut(s) 76, 266, 747, 807
CviQI GTAC 1 cut(s) 752
DpnI GATC 7 cut(s) 143, 261, 285, 290, 331, 362, 461
DpnII GATC 7 cut(s) 141, 259, 283, 288, 329, 360, 459
DraIII CACNNNGTG 1 cut(s) 749
Eam1104I CTCTTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 786
EciI GGCGGA 1 cut(s) 527
Eco130I CCWWGG 2 cut(s) 75, 96
Eco147I AGGCCT 2 cut(s) 164, 176
Eco24I GRGCYC 2 cut(s) 308, 328
Eco47I GGWCC 1 cut(s) 759
Eco57I CTGAAG 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 424
EcoNI CCTNNNNNAGG 2 cut(s) 158, 371
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 2 cut(s) 22, 366
EcoT14I CCWWGG 2 cut(s) 75, 96
EcoT38I GRGCYC 2 cut(s) 308, 328
ErhI CCWWGG 2 cut(s) 75, 96
FaeI CATG 4 cut(s) 79, 269, 750, 810
FaiI YATR 9 cut(s) 14, 77, 267, 726, 748, 777, 799, 801, 808
FatI CATG 4 cut(s) 75, 265, 746, 806
FauI CCCGC 1 cut(s) 53
FbaI TGATCA 1 cut(s) 459
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 2 cut(s) 57, 589
FokI GGATG 4 cut(s) 383, 527, 636, 797
FriOI GRGCYC 2 cut(s) 308, 328
Fsp4HI GCNGC 2 cut(s) 57, 589
FspBI CTAG 1 cut(s) 483
GlaI GCGC 2 cut(s) 41, 43
GluI GCNGC 2 cut(s) 57, 589
HaeIII GGCC 3 cut(s) 164, 176, 694
HapII CCGG 5 cut(s) 228, 255, 551, 560, 691
HhaI GCGC 2 cut(s) 42, 44
Hin1II CATG 4 cut(s) 79, 269, 750, 810
Hin6I GCGC 2 cut(s) 40, 42
HinP1I GCGC 2 cut(s) 40, 42
HincII GTYRAC 2 cut(s) 418, 472
HindII GTYRAC 2 cut(s) 418, 472
HinfI GANTC 7 cut(s) 19, 27, 68, 410, 440, 578, 734
HpaII CCGG 5 cut(s) 228, 255, 551, 560, 691
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 3 cut(s) 418, 472, 752
Hpy188I TCNGA 7 cut(s) 288, 303, 343, 360, 400, 445, 595
Hpy188III TCNNGA 5 cut(s) 191, 206, 219, 407, 823
Hpy8I GTNNAC 3 cut(s) 418, 472, 752
Hpy99I CGWCG 6 cut(s) 207, 347, 419, 434, 437, 599
HpyAV CCTTC 6 cut(s) 42, 132, 175, 187, 227, 314
HpyCH4III ACNGT 2 cut(s) 508, 707
HpyCH4V TGCA 1 cut(s) 278
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 182, 251, 275, 323
Hsp92II CATG 4 cut(s) 79, 269, 750, 810
HspAI GCGC 2 cut(s) 40, 42
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 7 cut(s) 141, 259, 283, 288, 329, 360, 459
LmnI GCTCC 4 cut(s) 303, 323, 400, 596
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 4 cut(s) 14, 172, 246, 343
MaeI CTAG 1 cut(s) 483
MaeIII GTNAC 3 cut(s) 65, 424, 731
MalI GATC 7 cut(s) 143, 261, 285, 290, 331, 362, 461
MboI GATC 7 cut(s) 141, 259, 283, 288, 329, 360, 459
MboII GAAGA 3 cut(s) 486, 800, 803
MfeI CAATTG 1 cut(s) 279
MhlI GDGCHC 2 cut(s) 308, 328
MluCI AATT 3 cut(s) 197, 279, 671
MlyI GAGTC 3 cut(s) 13, 62, 728
MmeI TCCRAC 2 cut(s) 366, 618
MroNI GCCGGC 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 1 cut(s) 765
MslI CAYNNNNRTG 4 cut(s) 455, 663, 723, 774
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 5 cut(s) 228, 255, 551, 560, 691
MspR9I CCNGG 5 cut(s) 24, 229, 368, 551, 691
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 2 cut(s) 24, 368
MvnI CGCG 2 cut(s) 42, 87
MwoI GCNNNNNNNGC 5 cut(s) 110, 182, 251, 275, 323
NaeI GCCGGC 1 cut(s) 256
NciI CCSGG 3 cut(s) 229, 551, 691
NcoI CCATGG 1 cut(s) 75
NdeII GATC 7 cut(s) 141, 259, 283, 288, 329, 360, 459
NgoMIV GCCGGC 1 cut(s) 254
NlaIII CATG 4 cut(s) 79, 269, 750, 810
NlaIV GGNNCC 6 cut(s) 226, 305, 325, 592, 695, 744
NmuCI GTSAC 2 cut(s) 65, 731
NspV TTCGAA 2 cut(s) 168, 497
PaeR7I CTCGAG 1 cut(s) 219
PauI GCGCGC 1 cut(s) 40
PceI AGGCCT 2 cut(s) 164, 176
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PdiI GCCGGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 4 cut(s) 27, 410, 440, 578
PfoI TCCNGGA 1 cut(s) 22
PkrI GCNGC 2 cut(s) 58, 590
Ple19I CGATCG 2 cut(s) 144, 332
PleI GAGTC 3 cut(s) 13, 62, 728
PpsI GAGTC 3 cut(s) 13, 62, 728
Psp6I CCWGG 2 cut(s) 22, 366
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 22, 366
PspN4I GGNNCC 6 cut(s) 226, 305, 325, 592, 695, 744
PspPI GGNCC 2 cut(s) 693, 759
PteI GCGCGC 1 cut(s) 40
PvuI CGATCG 2 cut(s) 144, 332
RsaI GTAC 1 cut(s) 753
RsaNI GTAC 1 cut(s) 752
RseI CAYNNNNRTG 4 cut(s) 455, 663, 723, 774
SalI GTCGAC 1 cut(s) 416
SaqAI TTAA 1 cut(s) 765
SatI GCNGC 2 cut(s) 57, 589
Sau3AI GATC 7 cut(s) 141, 259, 283, 288, 329, 360, 459
Sau96I GGNCC 2 cut(s) 693, 759
SchI GAGTC 3 cut(s) 13, 62, 728
ScrFI CCNGG 5 cut(s) 24, 229, 368, 551, 691
SduI GDGCHC 2 cut(s) 308, 328
SfaNI GCATC 4 cut(s) 14, 172, 246, 343
Sfr274I CTCGAG 1 cut(s) 219
SfuI TTCGAA 2 cut(s) 168, 497
SinI GGWCC 1 cut(s) 759
SlaI CTCGAG 1 cut(s) 219
SmiMI CAYNNNNRTG 4 cut(s) 455, 663, 723, 774
SmlI CTYRAG 1 cut(s) 219
SmoI CTYRAG 1 cut(s) 219
Sse9I AATT 3 cut(s) 197, 279, 671
SseBI AGGCCT 2 cut(s) 164, 176
SsiI CCGC 4 cut(s) 46, 54, 512, 588
SspI AATATT 1 cut(s) 819
SspMI CTAG 1 cut(s) 483
StuI AGGCCT 2 cut(s) 164, 176
StyD4I CCNGG 5 cut(s) 22, 227, 366, 549, 689
StyI CCWWGG 2 cut(s) 75, 96
TaaI ACNGT 2 cut(s) 508, 707
TaqI TCGA 8 cut(s) 17, 144, 168, 180, 220, 363, 417, 497
TasI AATT 3 cut(s) 197, 279, 671
TatI WGTACW 1 cut(s) 751
TauI GCSGC 1 cut(s) 591
TfiI GAWTC 4 cut(s) 27, 410, 440, 578
Tru1I TTAA 1 cut(s) 765
Tru9I TTAA 1 cut(s) 765
TscAI CASTG 3 cut(s) 142, 469, 710
TseFI GTSAC 2 cut(s) 65, 731
TseI GCWGC 1 cut(s) 56
Tsp45I GTSAC 2 cut(s) 65, 731
TspDTI ATGAA 1 cut(s) 801
TspGWI ACGGA 3 cut(s) 403, 517, 797
TspRI CASTG 3 cut(s) 142, 469, 710
VpaK11BI GGWCC 1 cut(s) 759
XagI CCTNNNNNAGG 2 cut(s) 158, 371
XapI RAATTY 2 cut(s) 197, 671
XcmI CCANNNNNNNNNTGG 1 cut(s) 753
XhoI CTCGAG 1 cut(s) 219
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.