pycom10g15800

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006341
Physical Location & Seq
Forward (+)
18983695 .. 18987737
4043 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2490 bp
ATGGATACTTTTCGACCAAAGTTCGATCTCAACTCAGAGGAGTCTGACTGTGAACAGTTTAATACGGAACCCGCTACAAAATATTGCCTTCCGAAGGGTGTTACTCACGGATGTCCAGATTGCAGTGGTTGCCTGAAGGTGACTGGAAGGTGGCGTCCTGAAGATGCAAGAACAGATGTCCTTGAAGAAGCACCTCTTTTCTACCCTACAGAAGAGGAATTTAAAGACACTTTTAAGTATGTTGCAAATATACGTGCAAGAGCAGAGCAATATGGTATCTGTCGTATTATTCCTCCTCCTTCCTGGAAACCTCCATGTCTTATTGAAGGAAACAAAATATGGAAAAGTTCTACATTTTCTACCCATATTCAGCGAATTGATGGGATACGAAATCAGTATTCACTGAGTAAAATGGTTGGCTTAAATGAAAGCCCAAAAAAGAAGAGGAGAAGAATCTTGACAGTGGGATTGGACTGTGGATCTACCTCAAGTCCAGGTGAAACTGAACATTCTTATATCAAAGGTTTTGAAACAGAACCTGGACAAGAATTCACTCTTGAGAATTTTAAGAGTTATGCAGCTGATTTCAAGCGTCAGTATTTCCGCAAAAGTGAAGTCACTGGTGGTCAAGAGAAGTGGGTGCCATCGCTGGAGAATATTGACGCTGAATATAAACGTATAGCTGAAAACCCAACTGAAGAAATTGAGGTGCTTTTTGGTGATAATTTGGAGACTAAAGCTCTTGGAAGCGGATTTCCAACAGTATCCAGTGATTCCAATCCTTTGGAAAAGTCAGATTACCCTGAATATTTAGCTTCAGGTTGGAACCTAAATAATCTACCCAGATTACCCGGTTCTCTTCTTTCTTTTGAAAGCCATGACACTTGCCATATTTTGGCGCCTCGGACTCGTGTAGGAATGTGCTTTTCATCTTTTCATTGGAAAGTTGAAGAGCACCGCTTATACTCACTGTCTTACTTGCATCTGGGTGCTCCTAAAATTTGGTATGGTGTCCCTGGTAAATACTATGTTAATTTCGAGGCAACCGTGAGGAGCTCCTTTCCAGATTCATCCAAACTAGAACACCCTGAATTGCATCATATGCTGGTTAAACAAATATCCCCGTCTGCATTGAAATCACAGGGCGTACCAGTCTTCCGTTGCATTCAGTCTCCCGGGGAGTTTGTTCTTGTCCTTCCAAGGGCTTATCATTCAGGATTTGATTCTGGTTTTAATTTTTCCGAGACAGCATGTGTTGCACCCTTGGACTGGTTGCCTCATGGACAGGATGCCGTTGAACTTTACTGTGAACAGGGGAGGAGAACATCTATATCACATGATAAGCTGCTGCTCGGAGCAGCTAGAGAGGCTGTGAGGGCACAATGGGATTCACTGTTCAGGAAGAACGCTGCGGATAATATATTCTGGAAAAATGTCTGTGGAAAGGATGGGATCTTAACACATGCATTCAAGTCGCGCCTCAAGTCTGAAGGCAGTAGAAGGAAATATCTCTTCAACTCTGCAAAATCAGAGAGGATGAAATCAAATTTTAATGTTACAAGCAAAAGGGAATGCAGCATTTGTCTCTGTGATCTGCATTTTTCTGCAGTGGTTTGTTCTTGTTGTGAAGGCAGATATTCGTGTCTGCTACACGCAAAGCAGCTCTGTTCTTGTGCTTGGAGTGACAAGATTTTCCTCTACCGTCACGAGATCTGTGATTTACAGCTACTTGTTGAAGCTTTGGAAGAAAAGTTTGACGCAGTCTACAAATGGGGCAAAGATGATCTCGGATTGACACTGCACGCGCATATTCCCAAGAACAGTCAAAATTCACCGGGGCATGTGGAGGGTGACTCTGACGCCGATAAACAGAAAGAGTCCATTTCTCGAATCAGGGAAGAAGTGAAGGCACGGATTCTGCGATCTACAACCTCAAACAAATCAAATTCAAATGATTACCCAACAGGATCCCTTGACTCTGCAACAGCCACTGGAAACGGTAAGAGTTCAGTCTCTAGCATCATGGATAAGGTGAAGGCGCATGCACTCCTCCGATCAACAATCTTCAATGAACTGAAAACAAAGGAGAATATAGTAGGACCTGCCATCGGCACAAACACAACAGGAAGCAATGCTACTCTTACGCCGAAAGTGGCTGCAGAGGGAACAACGAATAATGCTTCTCTTCTACCGAAAGAGGCGATAGACATGATGGAGGGGACATCCGATGTTTCATCTGCAAGTACTAGTGAAACAAGCTCCTCAGACTCCGACGATGTAATACCAGATCTTAACATTGTTCTGCGCGGAAGACAAAAAGTTCCCTCCCCTCCGGTGAAAAGCAGTGTTCCTCAAAAGCTTCCGAATGGTGGTCGTCCGGCCGACAATTACAAGGCATCTTCTTCGAGCCATTTGGCTTCCAAGAATCAAACCCCGAGACAACCATGTCCTCAGAGTCCAATTCTTATTAGTGATGACAGCGATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

830

Amino Acids

92.24

Weight (kDa)

6.91

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 65 - 98 4.8e-15 jmjN domain
JmjC PF02373 305 - 416 1e-30 JmjC domain, hydroxylase
zf-C5HC2 PF02928 525 - 575 1.2e-11 C5HC2 zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2445
Acc36I ACCTGC 1 cut(s) 2110
AccB1I GGYRCC 2 cut(s) 640, 898
AccB7I CCANNNNNTGG 1 cut(s) 895
AccI GTMKAC 1 cut(s) 1764
AccII CGCG 3 cut(s) 1477, 1805, 2307
AciI CCGC 6 cut(s) 72, 604, 750, 958, 1412, 2307
AclWI GGATC 4 cut(s) 487, 1460, 1962, 1975
AcoI YGGCCR 1 cut(s) 2379
AcsI RAATTY 7 cut(s) 218, 548, 562, 999, 1546, 1828, 1945
AcuI CTGAAG 5 cut(s) 155, 180, 717, 801, 1509
AcyI GRCGYC 3 cut(s) 154, 899, 1860
AfaI GTAC 2 cut(s) 1149, 2245
AfiI CCNNNNNNNGG 6 cut(s) 94, 895, 1201, 1269, 2108, 2369
AhlI ACTAGT 1 cut(s) 2246
AjnI CCWGG 4 cut(s) 302, 493, 538, 1015
AloI GAACNNNNNNTCC 2 cut(s) 1379, 1411
Alw21I GWGCWC 3 cut(s) 957, 994, 1058
Alw26I GTCTC 6 cut(s) 725, 1176, 1238, 1589, 2017, 2431
AlwI GGATC 4 cut(s) 487, 1460, 1962, 1975
AlwNI CAGNNNCTG 2 cut(s) 539, 1991
Ama87I CYCGRG 2 cut(s) 1175, 2434
AoxI GGCC 1 cut(s) 2379
ApeKI GCWGC 8 cut(s) 578, 1345, 1348, 1358, 1409, 1575, 1660, 2156
ApoI RAATTY 7 cut(s) 218, 548, 562, 999, 1546, 1828, 1945
ArsI GACNNNNNNTTYG 2 cut(s) 600, 632
AspLEI GCGC 5 cut(s) 901, 1479, 1807, 2041, 2307
AspS9I GGNCC 1 cut(s) 2099
AsuC2I CCSGG 4 cut(s) 852, 1176, 1177, 1836
AsuHPI GGTGA 7 cut(s) 151, 509, 731, 1824, 1862, 2044, 2347
AvaI CYCGRG 2 cut(s) 1175, 2434
AvaII GGWCC 1 cut(s) 2099
BaeGI GKGCMC 1 cut(s) 1381
BamHI GGATCC 1 cut(s) 1967
BanI GGYRCC 2 cut(s) 640, 898
BanII GRGCYC 1 cut(s) 1058
BarI GAAGNNNNNNTAC 2 cut(s) 2119, 2151
BauI CACGAG 2 cut(s) 909, 1706
BbsI GAAGAC 2 cut(s) 1147, 2317
Bbv12I GWGCWC 3 cut(s) 957, 994, 1058
BbvI GCAGC 8 cut(s) 590, 1332, 1335, 1370, 1396, 1587, 1672, 2143
BccI CCATC 6 cut(s) 374, 652, 1442, 2114, 2206, 2477
BceAI ACGGC 1 cut(s) 1277
BcgI CGANNNNNNTGC 4 cut(s) 1455, 1489, 2385, 2419
BciT130I CCWGG 4 cut(s) 304, 495, 540, 1017
BciVI GTATCC 2 cut(s) 378, 775
BcnI CCSGG 4 cut(s) 852, 1176, 1177, 1836
BcoDI GTCTC 6 cut(s) 725, 1176, 1238, 1589, 2017, 2431
BcuI ACTAGT 1 cut(s) 2246
BfaI CTAG 4 cut(s) 1079, 1362, 2016, 2247
BfmI CTRYAG 3 cut(s) 207, 1605, 2157
BfoI RGCGCY 1 cut(s) 902
BfuAI ACCTGC 1 cut(s) 2110
BfuI GTATCC 2 cut(s) 378, 775
BglII AGATCT 2 cut(s) 1710, 2287
BisI GCNGC 8 cut(s) 579, 1346, 1349, 1359, 1410, 1576, 1661, 2157
BlsI GCNGC 8 cut(s) 580, 1347, 1350, 1360, 1411, 1577, 1662, 2158
BmcAI AGTACT 1 cut(s) 2245
Bme1390I CCNGG 8 cut(s) 304, 495, 540, 852, 1017, 1176, 1177, 1836
Bme18I GGWCC 1 cut(s) 2099
BmeT110I CYCGRG 2 cut(s) 1175, 2434
BmgT120I GGNCC 1 cut(s) 2099
BmiI GGNNCC 5 cut(s) 69, 642, 827, 900, 1969
BmrFI CCNGG 8 cut(s) 304, 495, 540, 852, 1017, 1176, 1177, 1836
BmsI GCATC 6 cut(s) 154, 991, 1105, 1279, 2028, 2405
BpiI GAAGAC 2 cut(s) 1147, 2317
BplI GAGNNNNNCTC 2 cut(s) 1838, 1870
BpmI CTGGAG 1 cut(s) 671
BpuEI CTTGAG 3 cut(s) 472, 578, 1466
BpuMI CCSGG 4 cut(s) 852, 1176, 1177, 1836
BsaAI YACGTR 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 777
BsaHI GRCGYC 3 cut(s) 154, 899, 1860
BsaJI CCNNGG 7 cut(s) 902, 1015, 1175, 1176, 1199, 1263, 1835
BsaWI WCCGGW 1 cut(s) 2332
Bsc4I CCNNNNNNNGG 6 cut(s) 94, 895, 1201, 1269, 2108, 2369
Bse1I ACTGG 6 cut(s) 148, 625, 768, 1151, 1274, 1996
Bse3DI GCAATG 1 cut(s) 2137
Bse8I GATNNNNATC 1 cut(s) 777
BseBI CCWGG 4 cut(s) 304, 495, 540, 1017
BseDI CCNNGG 7 cut(s) 902, 1015, 1175, 1176, 1199, 1263, 1835
BseGI GGATG 6 cut(s) 116, 1070, 1294, 1453, 1542, 2222
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 6 cut(s) 94, 895, 1201, 1269, 2108, 2369
BseMI GCAATG 1 cut(s) 2137
BseMII CTCAG 4 cut(s) 48, 395, 2277, 2465
BseNI ACTGG 6 cut(s) 148, 625, 768, 1151, 1274, 1996
BseRI GAGGAG 7 cut(s) 53, 285, 460, 1066, 1333, 2039, 2251
BseSI GKGCMC 1 cut(s) 1381
BseX3I CGGCCG 1 cut(s) 2379
BseXI GCAGC 8 cut(s) 590, 1332, 1335, 1370, 1396, 1587, 1672, 2143
BsgI GTGCAG 1 cut(s) 1784
Bsh1236I CGCG 3 cut(s) 1477, 1805, 2307
Bsh1285I CGRYCG 1 cut(s) 2382
BshFI GGCC 1 cut(s) 2381
BshNI GGYRCC 2 cut(s) 640, 898
BsiEI CGRYCG 1 cut(s) 2382
BsiHKAI GWGCWC 3 cut(s) 957, 994, 1058
BsiHKCI CYCGRG 2 cut(s) 1175, 2434
BsiSI CCGG 5 cut(s) 852, 1176, 1835, 2333, 2378
BslFI GGGAC 2 cut(s) 998, 2233
BslI CCNNNNNNNGG 6 cut(s) 94, 895, 1201, 1269, 2108, 2369
BsmAI GTCTC 6 cut(s) 725, 1176, 1238, 1589, 2017, 2431
BsmFI GGGAC 2 cut(s) 998, 2233
BsmI GAATGC 3 cut(s) 1164, 1466, 1577
BsnI GGCC 1 cut(s) 2381
BsoBI CYCGRG 2 cut(s) 1175, 2434
Bsp1286I GDGCHC 4 cut(s) 957, 994, 1058, 1381
BspACI CCGC 6 cut(s) 72, 604, 750, 958, 1412, 2307
BspANI GGCC 1 cut(s) 2381
BspCNI CTCAG 4 cut(s) 47, 396, 2276, 2464
BspFNI CGCG 3 cut(s) 1477, 1805, 2307
BspLI GGNNCC 5 cut(s) 69, 642, 827, 900, 1969
BspMAI CTGCAG 2 cut(s) 1609, 2161
BspMI ACCTGC 1 cut(s) 2110
BspPI GGATC 4 cut(s) 487, 1460, 1962, 1975
BspQI GCTCTTC 1 cut(s) 945
BspT107I GGYRCC 2 cut(s) 640, 898
BsrDI GCAATG 1 cut(s) 2137
BsrI ACTGG 6 cut(s) 148, 625, 768, 1151, 1274, 1996
BssECI CCNNGG 7 cut(s) 902, 1015, 1175, 1176, 1199, 1263, 1835
BssNI GRCGYC 3 cut(s) 154, 899, 1860
BssSI CACGAG 2 cut(s) 909, 1706
BssT1I CCWWGG 2 cut(s) 1199, 1263
Bst2BI CACGAG 2 cut(s) 909, 1706
Bst2UI CCWGG 4 cut(s) 304, 495, 540, 1017
Bst6I CTCTTC 6 cut(s) 207, 437, 864, 945, 1517, 2190
BstACI GRCGYC 3 cut(s) 154, 899, 1860
BstAPI GCANNNNNTGC 3 cut(s) 129, 1102, 1256
BstBAI YACGTR 1 cut(s) 254
BstC8I GCNNGC 2 cut(s) 1803, 2043
BstDEI CTNAG 4 cut(s) 34, 404, 2263, 2451
BstENI CCTNNNNNAGG 1 cut(s) 92
BstF5I GGATG 6 cut(s) 116, 1070, 1294, 1453, 1542, 2222
BstFNI CGCG 3 cut(s) 1477, 1805, 2307
BstH2I RGCGCY 1 cut(s) 902
BstHHI GCGC 5 cut(s) 901, 1479, 1807, 2041, 2307
BstMAI GTCTC 6 cut(s) 725, 1176, 1238, 1589, 2017, 2431
BstMCI CGRYCG 1 cut(s) 2382
BstMWI GCNNNNNNNGC 5 cut(s) 129, 1102, 1256, 1367, 1376
BstNI CCWGG 4 cut(s) 304, 495, 540, 1017
BstNSI RCATGY 4 cut(s) 1254, 1466, 1844, 2045
BstSCI CCNGG 8 cut(s) 302, 493, 538, 850, 1015, 1174, 1175, 1834
BstSFI CTRYAG 3 cut(s) 207, 1605, 2157
BstSLI GKGCMC 1 cut(s) 1381
BstUI CGCG 3 cut(s) 1477, 1805, 2307
BstV1I GCAGC 8 cut(s) 590, 1332, 1335, 1370, 1396, 1587, 1672, 2143
BstV2I GAAGAC 2 cut(s) 1147, 2317
BstX2I RGATCY 5 cut(s) 479, 1452, 1710, 1967, 2287
BstXI CCANNNNNNTGG 1 cut(s) 784
BstYI RGATCY 5 cut(s) 479, 1452, 1710, 1967, 2287
BstZI CGGCCG 1 cut(s) 2379
BsuI GTATCC 2 cut(s) 378, 775
BsuRI GGCC 1 cut(s) 2381
BtgZI GCGATG 1 cut(s) 630
BtsCI GGATG 6 cut(s) 116, 1070, 1294, 1453, 1542, 2222
BtsI GCAGTG 4 cut(s) 130, 1614, 1796, 2350
BveI ACCTGC 1 cut(s) 2110
Cac8I GCNNGC 2 cut(s) 1803, 2043
CaiI CAGNNNCTG 2 cut(s) 539, 1991
CfoI GCGC 5 cut(s) 901, 1479, 1807, 2041, 2307
Cfr13I GGNCC 1 cut(s) 2099
Cfr9I CCCGGG 1 cut(s) 1175
CseI GACGC 5 cut(s) 143, 581, 671, 1766, 1868
Csp6I GTAC 2 cut(s) 1148, 2244
CspCI CAANNNNNGTGG 2 cut(s) 617, 652
CviQI GTAC 2 cut(s) 1148, 2244
DdeI CTNAG 4 cut(s) 34, 404, 2263, 2451
DinI GGCGCC 1 cut(s) 900
DraI TTTAAA 1 cut(s) 223
DrdI GACNNNNNNGTC 1 cut(s) 2445
DseDI GACNNNNNNGTC 1 cut(s) 2445
EaeI YGGCCR 1 cut(s) 2379
EagI CGGCCG 1 cut(s) 2379
Eam1104I CTCTTC 6 cut(s) 207, 437, 864, 945, 1517, 2190
EarI CTCTTC 6 cut(s) 207, 437, 864, 945, 1517, 2190
Ecl136II GAGCTC 1 cut(s) 1056
EclXI CGGCCG 1 cut(s) 2379
Eco130I CCWWGG 2 cut(s) 1199, 1263
Eco24I GRGCYC 1 cut(s) 1058
Eco47I GGWCC 1 cut(s) 2099
Eco52I CGGCCG 1 cut(s) 2379
Eco53kI GAGCTC 1 cut(s) 1056
Eco57I CTGAAG 5 cut(s) 155, 180, 717, 801, 1509
Eco88I CYCGRG 2 cut(s) 1175, 2434
EcoICRI GAGCTC 1 cut(s) 1056
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 2099
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 4 cut(s) 302, 493, 538, 1015
EcoT14I CCWWGG 2 cut(s) 1199, 1263
EcoT22I ATGCAT 1 cut(s) 1468
EcoT38I GRGCYC 1 cut(s) 1058
EgeI GGCGCC 1 cut(s) 900
EheI GGCGCC 1 cut(s) 900
ErhI CCWWGG 2 cut(s) 1199, 1263
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 2 cut(s) 998, 2233
FauI CCCGC 1 cut(s) 79
FauNDI CATATG 1 cut(s) 1101
FblI GTMKAC 1 cut(s) 1764
Fnu4HI GCNGC 8 cut(s) 579, 1346, 1349, 1359, 1410, 1576, 1661, 2157
FokI GGATG 6 cut(s) 123, 1057, 1301, 1460, 1549, 2209
FriOI GRGCYC 1 cut(s) 1058
Fsp4HI GCNGC 8 cut(s) 579, 1346, 1349, 1359, 1410, 1576, 1661, 2157
FspBI CTAG 4 cut(s) 1079, 1362, 2016, 2247
GlaI GCGC 5 cut(s) 900, 1478, 1806, 2040, 2306
GluI GCNGC 8 cut(s) 579, 1346, 1349, 1359, 1410, 1576, 1661, 2157
GsuI CTGGAG 1 cut(s) 671
HaeII RGCGCY 1 cut(s) 902
HaeIII GGCC 1 cut(s) 2381
HapII CCGG 5 cut(s) 852, 1176, 1835, 2333, 2378
HgaI GACGC 5 cut(s) 143, 581, 671, 1766, 1868
HhaI GCGC 5 cut(s) 901, 1479, 1807, 2041, 2307
Hin1I GRCGYC 3 cut(s) 154, 899, 1860
Hin6I GCGC 5 cut(s) 899, 1477, 1805, 2039, 2305
HinP1I GCGC 5 cut(s) 899, 1477, 1805, 2039, 2305
HindIII AAGCTT 2 cut(s) 1737, 2357
HpaII CCGG 5 cut(s) 852, 1176, 1835, 2333, 2378
HphI GGTGA 7 cut(s) 151, 509, 731, 1824, 1862, 2044, 2347
Hpy166II GTNNAC 3 cut(s) 53, 1310, 1765
Hpy8I GTNNAC 3 cut(s) 53, 1310, 1765
Hpy99I CGWCG 1 cut(s) 2276
HpyCH4IV ACGT 2 cut(s) 253, 676
HpyF10VI GCNNNNNNNGC 5 cut(s) 129, 1102, 1256, 1367, 1376
HpyF3I CTNAG 4 cut(s) 34, 404, 2263, 2451
HpySE526I ACGT 2 cut(s) 253, 676
Hsp92I GRCGYC 3 cut(s) 154, 899, 1860
HspAI GCGC 5 cut(s) 899, 1477, 1805, 2039, 2305
KasI GGCGCC 1 cut(s) 898
LguI GCTCTTC 1 cut(s) 945
LmnI GCTCC 5 cut(s) 997, 1053, 1061, 1355, 2264
Lsp1109I GCAGC 8 cut(s) 590, 1332, 1335, 1370, 1396, 1587, 1672, 2143
LweI GCATC 6 cut(s) 154, 991, 1105, 1279, 2028, 2405
MaeI CTAG 4 cut(s) 1079, 1362, 2016, 2247
MaeII ACGT 2 cut(s) 253, 676
MaeIII GTNAC 7 cut(s) 100, 139, 616, 1555, 1682, 1703, 1850
MflI RGATCY 5 cut(s) 479, 1452, 1710, 1967, 2287
MhlI GDGCHC 4 cut(s) 957, 994, 1058, 1381
Mly113I GGCGCC 1 cut(s) 899
MlyI GAGTC 7 cut(s) 50, 901, 1847, 1886, 1970, 2261, 2464
MmeI TCCRAC 3 cut(s) 782, 803, 2295
Mph1103I ATGCAT 1 cut(s) 1468
MslI CAYNNNNRTG 1 cut(s) 987
MspA1I CMGCKG 1 cut(s) 581
MspI CCGG 5 cut(s) 852, 1176, 1835, 2333, 2378
MspR9I CCNGG 8 cut(s) 304, 495, 540, 852, 1017, 1176, 1177, 1836
Mva1269I GAATGC 3 cut(s) 1164, 1466, 1577
MvaI CCWGG 4 cut(s) 304, 495, 540, 1017
MvnI CGCG 3 cut(s) 1477, 1805, 2307
MwoI GCNNNNNNNGC 5 cut(s) 129, 1102, 1256, 1367, 1376
NarI GGCGCC 1 cut(s) 899
NciI CCSGG 4 cut(s) 852, 1176, 1177, 1836
NdeI CATATG 1 cut(s) 1101
NlaIV GGNNCC 5 cut(s) 69, 642, 827, 900, 1969
NmuCI GTSAC 5 cut(s) 139, 616, 1682, 1703, 1850
NsiI ATGCAT 1 cut(s) 1468
NspI RCATGY 4 cut(s) 1254, 1466, 1844, 2045
PaeI GCATGC 1 cut(s) 2045
PciSI GCTCTTC 1 cut(s) 945
PcsI WCGNNNNNNNCGW 2 cut(s) 1044, 1918
PctI GAATGC 3 cut(s) 1164, 1466, 1577
PfeI GAWTC 8 cut(s) 453, 773, 1067, 1223, 1388, 1890, 1915, 2425
PflFI GACNNNGTC 1 cut(s) 1760
PflMI CCANNNNNTGG 1 cut(s) 895
PfoI TCCNGGA 1 cut(s) 302
PkrI GCNGC 8 cut(s) 580, 1347, 1350, 1360, 1411, 1577, 1662, 2158
PleI GAGTC 7 cut(s) 49, 901, 1847, 1885, 1970, 2261, 2463
PluTI GGCGCC 1 cut(s) 902
PpsI GAGTC 7 cut(s) 49, 901, 1847, 1885, 1970, 2261, 2463
Ppu21I YACGTR 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 2099
Psp124BI GAGCTC 1 cut(s) 1058
Psp5II RGGWCCY 1 cut(s) 2099
Psp6I CCWGG 4 cut(s) 302, 493, 538, 1015
PspGI CCWGG 4 cut(s) 302, 493, 538, 1015
PspN4I GGNNCC 5 cut(s) 69, 642, 827, 900, 1969
PspPI GGNCC 1 cut(s) 2099
PspPPI RGGWCCY 1 cut(s) 2099
PstI CTGCAG 2 cut(s) 1609, 2161
PstNI CAGNNNCTG 2 cut(s) 539, 1991
PsuI RGATCY 5 cut(s) 479, 1452, 1710, 1967, 2287
PsyI GACNNNGTC 1 cut(s) 1760
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 2 cut(s) 1149, 2245
RsaNI GTAC 2 cut(s) 1148, 2244
RseI CAYNNNNRTG 1 cut(s) 987
SacI GAGCTC 1 cut(s) 1058
SapI GCTCTTC 1 cut(s) 945
SatI GCNGC 8 cut(s) 579, 1346, 1349, 1359, 1410, 1576, 1661, 2157
Sau96I GGNCC 1 cut(s) 2099
ScaI AGTACT 1 cut(s) 2245
SchI GAGTC 7 cut(s) 50, 901, 1847, 1886, 1970, 2261, 2464
ScrFI CCNGG 8 cut(s) 304, 495, 540, 852, 1017, 1176, 1177, 1836
SduI GDGCHC 4 cut(s) 957, 994, 1058, 1381
SfaNI GCATC 6 cut(s) 154, 991, 1105, 1279, 2028, 2405
SfcI CTRYAG 3 cut(s) 207, 1605, 2157
SfoI GGCGCC 1 cut(s) 900
SinI GGWCC 1 cut(s) 2099
SmaI CCCGGG 1 cut(s) 1177
SmiMI CAYNNNNRTG 1 cut(s) 987
SmlI CTYRAG 3 cut(s) 487, 557, 1481
SmoI CTYRAG 3 cut(s) 487, 557, 1481
SpeI ACTAGT 1 cut(s) 2246
SphI GCATGC 1 cut(s) 2045
SsiI CCGC 6 cut(s) 72, 604, 750, 958, 1412, 2307
SspDI GGCGCC 1 cut(s) 898
SspI AATATT 3 cut(s) 83, 658, 809
SspMI CTAG 4 cut(s) 1079, 1362, 2016, 2247
SstI GAGCTC 1 cut(s) 1058
StyD4I CCNGG 8 cut(s) 302, 493, 538, 850, 1015, 1174, 1175, 1834
StyI CCWWGG 2 cut(s) 1199, 1263
TaiI ACGT 2 cut(s) 256, 679
TaqI TCGA 5 cut(s) 13, 24, 1038, 1888, 2405
TatI WGTACW 1 cut(s) 2243
TfiI GAWTC 8 cut(s) 453, 773, 1067, 1223, 1388, 1890, 1915, 2425
TseFI GTSAC 5 cut(s) 139, 616, 1682, 1703, 1850
TseI GCWGC 8 cut(s) 578, 1345, 1348, 1358, 1409, 1575, 1660, 2156
Tsp45I GTSAC 5 cut(s) 139, 616, 1682, 1703, 1850
TspDTI ATGAA 7 cut(s) 441, 918, 926, 1059, 1553, 2085, 2223
TspGWI ACGGA 4 cut(s) 80, 123, 1148, 1927
TspMI CCCGGG 1 cut(s) 1175
Tth111I GACNNNGTC 1 cut(s) 1760
Van91I CCANNNNNTGG 1 cut(s) 895
VpaK11BI GGWCC 1 cut(s) 2099
XagI CCTNNNNNAGG 1 cut(s) 92
XapI RAATTY 7 cut(s) 218, 548, 562, 999, 1546, 1828, 1945
XceI RCATGY 4 cut(s) 1254, 1466, 1844, 2045
XmaI CCCGGG 1 cut(s) 1175
XmiI GTMKAC 1 cut(s) 1764
XspI CTAG 4 cut(s) 1079, 1362, 2016, 2247
ZrmI AGTACT 1 cut(s) 2245
Zsp2I ATGCAT 1 cut(s) 1468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.