pycom10g15870

Structural maintenance of chromosomes protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006326
Physical Location & Seq
Forward (+)
19061477 .. 19068359
6883 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2391 bp
ATGCCGTCCCTGGTCTCTCAGGGCAAAATCCTCCGCCTGGAGCTCGAGAACTTCAAGTCCTATAAGGGCTTCCAGACCATCGGTCCCTTCTACGACTTCACGGCGATTATTGGGCCCAATGGCGCTGGAAAGTCCAACCTCATGGACGCCATAAGCTTCGTCCTCGGCGTGCGGACGGGCCACCTCCGTGGGGCCCAGCTGAAGGACCTGATCGATGCCTTCAACGACAAGGAGAAGGACCAGAAGGGCCGCAGGGCCTTTGTCCGCTTGGTTTACCAGCTGGACAATGGGTCGGAGCTCCAGTTCACCCGAGCCATTACTGGCGGAGCGGGCAGCGAGTACCGCATTGATGGGGCGTCTGTGACGTGGGAAGAGTACAATGCGAAGCTCAGGTCTCTTGGGATTCTTGTCAAGGCGCGGAATTTCCTCGTCTTTCAGGGTGATGTGGAGTCCATTGCCTCCAAAAATCCCAAGGAACTCACTGCACTTCTTGAGCAGATATCTGGGTCTGATGAACTCAAGAGAGACTATGAAAAATATGAAGAAGAAAAATCCGAAGCTGAACAAAAAGCAAGTCTTGTTTATCAGAGAAAGAGAACAATAGTATTGGAGAGGAGGCAAAAGAAAGAACAAAAAGAAGAAGCTGAGAAACACCTCCGCCTGCAAGATCAACTGAAATCTTTGAAGAAAGAACATTCTTTATGGCAATTGTTCAACATAGAAAATGATATTGCAAAAATGACTGAGGAGCTTGAAGCTGAAAAGAAAAGCCGTGAACAAGTTATGCAAGAAATTGATGAGTTTCAGCTAGAAGCAAACAATAAGAAGAAAGAACAAGCAAAATATCTGAAAGAGATTGCACAATGTGAAAAGAAGATTTCAGATAGAAATAATAAACTTGACAAAAGTCAACCAGACCTTCTGAAACTGAAAGAGGAAATGTCTCGTATAAATGCAAAAATCAAGAAAAGTGAGAAGGAGCTCGCTAAGAAAGAACAAGAAAGGAAAAGACATAAAGAGGATATAAAGCAGCTCCAGAAGGGCATACATGATCTGACTGCAAAACTAGAAGATTTACATGAAAAAGGCCGAGATAGTGGCGACAAACTGAAGTTAGATGATACTGAATTAAGGGAATATTTTCGAATCAAGGAAGATGCTGGGATGAAAACTGCAAAGCTAAGAGATGAGAAAGAGGTTCTGGATAGGCAACAACATGCTGATCTTGAAGCTCAAAAGAATTTGGAAGAAAATCTTCAACAGTTGAGAAGTCGCGAGGCTGAATTAGAGGAACAAGAGGAACAAATGCAAACAAGGCAGAAAAAAATTAAAGAAAATTCTGCGAAACATAGGGATGAAGCTAAAAATCTGGATAATGAACTGCGTGCCATGCAATCTAAAAATTCACAGGCCAGAACAAAATATGAAAATCTGAAGTCAAAAATTGATGAAATAGAAAAGCAACTGCGGGAATTGAAGGCTGACAGATACGAGAATGAGAGAGATTCCAGGTTGTCTCAAGCAGTCGAGACTCTCAAACGCCTGTTTCACGGTGTCCATGGTCGTATGACTGAACTCTGTAGGCCAACACAAAAAAAGTATAACCTTGCTGTTACTGTTGCCATGGGTAAATTTATGGATGCCGTTGTAGTTGAGGATGAACAAACGGGAAAGGAATGCATCAAGTATTTGAAAGAACAAAGGCTTCCTCCTCAGACATTCATACCTCTTCAGTCTGTTCGTGTGAAGCCCGTTATGGAGCGATTGCGCAATTTAGGTGGTACTGCAAAGCTGATCGATCTCTTTGATGTAGTCCAATTTGATCCTGCCTTGGAGAAGGCAATTCTTTTTGCTGTTGGCAATACTCTTGTCTGTGATGAACTTGATGAGGCTAAGCGTCTCAGCTGGACTGGCGAGAGGTTCAAAGTTGTAACTGTTGATGGAATTCTGCTTGCGAAATCCGGCACAATGACTGGCGGTACCAGTGGTGGAATGGAAGCAAGGTCAAAACAGTGGGATGATAAAAAAGTTGAAGGATTGAAGAAGAAGAAAGAACAGTTTGAGTCAGAGTTGGAAGAGCTGGGGTCAATTAGAGAGATGCAGATAAAAGAGTCTGAGACAACTGGTAGAATTAGTGGACTTCAAAAAGAGATCCAGTATGCAGAGATTGAGAAGAAAAGCATTAAGGACAAACTTGCAACCTTGGCACGGGAAAAGCAGAATATAAAAGAAGAGATTGATCGCAGCAGTCCTGAACTTCTCAAGTTAAAGCAAGCTGTAGATAAGCGAAGCAAGGAAATCAATAAGCTTGAGAAGAGGATAAATGAAATTGTTGACAGAATCTACAAAGATTTTAGTAGATCGATCTGTTGGGGTGGCAAACATCCGTGA

Protein Analysis

797

Amino Acids

92.18

Weight (kDa)

8.9

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_15 PF13175 9 - 347 1.6e-15 AAA ATPase domain
SMC_N PF02463 10 - 355 1e-16 RecF/RecN/SMC N terminal domain
AAA_23 PF13476 12 - 231 1.2e-15 AAA domain
AAA_29 PF13555 12 - 55 4.6e-07 P-loop containing region of AAA domain
AAA_21 PF13304 33 - 63 1.9e-07 AAA domain, putative AbiEii toxin, Type IV TA system
SMC_hinge PF06470 516 - 634 2.2e-27 SMC proteins Flexible Hinge Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1769
Acc65I GGTACC 1 cut(s) 1979
AccB1I GGYRCC 1 cut(s) 1979
AccBSI CCGCTC 1 cut(s) 329
AccII CGCG 2 cut(s) 418, 1275
AclWI GGATC 2 cut(s) 1817, 2146
AcsI RAATTY 6 cut(s) 421, 1240, 1336, 1402, 1631, 1944
AcuI CTGAAG 4 cut(s) 221, 1130, 1454, 1715
AcyI GRCGYC 2 cut(s) 147, 356
AdeI CACNNNGTG 1 cut(s) 866
AfaI GTAC 4 cut(s) 341, 377, 1783, 1981
AfiI CCNNNNNNNGG 4 cut(s) 37, 190, 202, 2208
AhdI GACNNNNNGTC 2 cut(s) 81, 289
AjiI CACGTC 1 cut(s) 366
AjnI CCWGG 3 cut(s) 9, 36, 1508
AleI CACNNNNGTG 1 cut(s) 186
AloI GAACNNNNNNTCC 4 cut(s) 41, 73, 287, 319
Alw21I GWGCWC 3 cut(s) 45, 300, 984
Alw26I GTCTC 8 cut(s) 19, 399, 519, 948, 1521, 1523, 1904, 2111
AlwI GGATC 2 cut(s) 1817, 2146
Ama87I CYCGRG 2 cut(s) 44, 309
AoxI GGCC 8 cut(s) 113, 178, 192, 247, 255, 1087, 1410, 1583
ApaI GGGCCC 2 cut(s) 117, 196
ApeKI GCWGC 3 cut(s) 333, 1030, 2244
ApoI RAATTY 6 cut(s) 421, 1240, 1336, 1402, 1631, 1944
ArsI GACNNNNNNTTYG 4 cut(s) 377, 409, 559, 591
Asp700I GAANNNNTTC 2 cut(s) 1140, 1254
Asp718I GGTACC 1 cut(s) 1979
AspLEI GCGC 3 cut(s) 125, 418, 1770
AsuHPI GGTGA 2 cut(s) 298, 452
AsuII TTCGAA 1 cut(s) 1144
AvaI CYCGRG 2 cut(s) 44, 309
AvaII GGWCC 3 cut(s) 83, 205, 238
BaeGI GKGCMC 2 cut(s) 117, 196
BanI GGYRCC 1 cut(s) 1979
BanII GRGCYC 5 cut(s) 45, 117, 196, 300, 984
Bbv12I GWGCWC 3 cut(s) 45, 300, 984
BbvI GCAGC 3 cut(s) 345, 1042, 2256
BccI CCATC 3 cut(s) 86, 344, 1934
BceAI ACGGC 3 cut(s) 117, 756, 1628
BciT130I CCWGG 3 cut(s) 11, 38, 1510
BcoDI GTCTC 8 cut(s) 19, 399, 519, 948, 1521, 1523, 1904, 2111
BfaI CTAG 2 cut(s) 809, 1067
BfmI CTRYAG 2 cut(s) 1579, 2277
BfoI RGCGCY 1 cut(s) 126
BisI GCNGC 4 cut(s) 250, 334, 1031, 2245
BlpI GCTNAGC 1 cut(s) 1893
BlsI GCNGC 4 cut(s) 251, 335, 1032, 2246
Bme1390I CCNGG 3 cut(s) 11, 38, 1510
Bme18I GGWCC 3 cut(s) 83, 205, 238
BmeRI GACNNNNNGTC 2 cut(s) 81, 289
BmeT110I CYCGRG 2 cut(s) 44, 309
BmgBI CACGTC 1 cut(s) 366
BmiI GGNNCC 5 cut(s) 85, 115, 193, 194, 1981
BmrFI CCNGG 3 cut(s) 11, 38, 1510
BmsI GCATC 5 cut(s) 205, 1147, 1630, 1689, 2088
BoxI GACNNNNGTC 1 cut(s) 906
BpmI CTGGAG 3 cut(s) 59, 284, 1019
Bpu10I CCTNAGC 1 cut(s) 389
Bpu1102I GCTNAGC 1 cut(s) 1893
Bpu14I TTCGAA 1 cut(s) 1144
BpuEI CTTGAG 5 cut(s) 503, 512, 1503, 2246, 2330
Bsa29I ATCGAT 3 cut(s) 213, 1797, 2363
BsaHI GRCGYC 2 cut(s) 147, 356
BsaI GGTCTC 2 cut(s) 19, 399
BsaJI CCNNGG 8 cut(s) 9, 163, 187, 471, 1558, 1623, 1830, 2202
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 190, 202, 2208
Bse1I ACTGG 7 cut(s) 301, 325, 1915, 1978, 1983, 2128, 2155
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 3 cut(s) 11, 38, 1510
BseCI ATCGAT 3 cut(s) 213, 1797, 2363
BseDI CCNNGG 8 cut(s) 9, 163, 187, 471, 1558, 1623, 1830, 2202
BseGI GGATG 6 cut(s) 1170, 1360, 1645, 1663, 2023, 2383
BseLI CCNNNNNNNGG 4 cut(s) 37, 190, 202, 2208
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 7 cut(s) 32, 403, 636, 735, 1727, 1915, 2106
BseNI ACTGG 7 cut(s) 301, 325, 1915, 1978, 1983, 2128, 2155
BseRI GAGGAG 3 cut(s) 628, 761, 1701
BseSI GKGCMC 2 cut(s) 117, 196
BseXI GCAGC 3 cut(s) 345, 1042, 2256
BseYI CCCAGC 3 cut(s) 195, 1160, 2080
BsgI GTGCAG 1 cut(s) 468
Bsh1236I CGCG 2 cut(s) 418, 1275
BshFI GGCC 8 cut(s) 115, 180, 194, 249, 257, 1089, 1412, 1585
BshNI GGYRCC 1 cut(s) 1979
BshVI ATCGAT 3 cut(s) 213, 1797, 2363
BsiHKAI GWGCWC 3 cut(s) 45, 300, 984
BsiHKCI CYCGRG 2 cut(s) 44, 309
BsiSI CCGG 1 cut(s) 1962
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 4 cut(s) 37, 190, 202, 2208
BsmAI GTCTC 8 cut(s) 19, 399, 519, 948, 1521, 1523, 1904, 2111
BsmBI CGTCTC 1 cut(s) 1904
BsmFI GGGAC 1 cut(s) 69
BsmI GAATGC 1 cut(s) 1682
BsnI GGCC 8 cut(s) 115, 180, 194, 249, 257, 1089, 1412, 1585
Bso31I GGTCTC 2 cut(s) 19, 399
BsoBI CYCGRG 2 cut(s) 44, 309
Bsp119I TTCGAA 1 cut(s) 1144
Bsp120I GGGCCC 2 cut(s) 113, 192
Bsp1286I GDGCHC 5 cut(s) 45, 117, 196, 300, 984
Bsp1720I GCTNAGC 1 cut(s) 1893
Bsp19I CCATGG 2 cut(s) 1558, 1623
Bsp68I TCGCGA 1 cut(s) 1275
BspANI GGCC 8 cut(s) 115, 180, 194, 249, 257, 1089, 1412, 1585
BspCNI CTCAG 7 cut(s) 31, 402, 637, 736, 1726, 1914, 2107
BspDI ATCGAT 3 cut(s) 213, 1797, 2363
BspFNI CGCG 2 cut(s) 418, 1275
BspLI GGNNCC 5 cut(s) 85, 115, 193, 194, 1981
BspPI GGATC 2 cut(s) 1817, 2146
BspQI GCTCTTC 1 cut(s) 2070
BspT104I TTCGAA 1 cut(s) 1144
BspT107I GGYRCC 1 cut(s) 1979
BspTNI GGTCTC 2 cut(s) 19, 399
BsrBI CCGCTC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 7 cut(s) 301, 325, 1915, 1978, 1983, 2128, 2155
BssECI CCNNGG 8 cut(s) 9, 163, 187, 471, 1558, 1623, 1830, 2202
BssNI GRCGYC 2 cut(s) 147, 356
BssT1I CCWWGG 5 cut(s) 471, 1558, 1623, 1830, 2202
Bst2UI CCWGG 3 cut(s) 11, 38, 1510
Bst4CI ACNGT 6 cut(s) 1263, 1553, 1618, 1936, 2013, 2058
Bst6I CTCTTC 5 cut(s) 366, 1734, 2070, 2226, 2309
BstACI GRCGYC 2 cut(s) 147, 356
BstBI TTCGAA 1 cut(s) 1144
BstC8I GCNNGC 7 cut(s) 170, 331, 662, 984, 1386, 1953, 2274
BstDSI CCRYGG 3 cut(s) 187, 1558, 1623
BstF5I GGATG 6 cut(s) 1170, 1360, 1645, 1663, 2023, 2383
BstFNI CGCG 2 cut(s) 418, 1275
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 3 cut(s) 125, 418, 1770
BstMAI GTCTC 8 cut(s) 19, 399, 519, 948, 1521, 1523, 1904, 2111
BstMWI GCNNNNNNNGC 6 cut(s) 330, 342, 1315, 1390, 1911, 2204
BstNI CCWGG 3 cut(s) 11, 38, 1510
BstNSI RCATGY 1 cut(s) 1220
BstPAI GACNNNNGTC 1 cut(s) 906
BstSCI CCNGG 3 cut(s) 9, 36, 1508
BstSFI CTRYAG 2 cut(s) 1579, 2277
BstSLI GKGCMC 2 cut(s) 117, 196
BstUI CGCG 2 cut(s) 418, 1275
BstV1I GCAGC 3 cut(s) 345, 1042, 2256
BstX2I RGATCY 1 cut(s) 2151
BstXI CCANNNNNNTGG 2 cut(s) 142, 188
BstYI RGATCY 1 cut(s) 2151
Bsu15I ATCGAT 3 cut(s) 213, 1797, 2363
BsuRI GGCC 8 cut(s) 115, 180, 194, 249, 257, 1089, 1412, 1585
BsuTUI ATCGAT 3 cut(s) 213, 1797, 2363
BtgI CCRYGG 3 cut(s) 187, 1558, 1623
BtrI CACGTC 1 cut(s) 366
BtsCI GGATG 6 cut(s) 1170, 1360, 1645, 1663, 2023, 2383
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 3 cut(s) 480, 1990, 2018
BtuMI TCGCGA 1 cut(s) 1275
Cac8I GCNNGC 7 cut(s) 170, 331, 662, 984, 1386, 1953, 2274
CfoI GCGC 3 cut(s) 125, 418, 1770
ClaI ATCGAT 3 cut(s) 213, 1797, 2363
CseI GACGC 3 cut(s) 155, 345, 1886
Csp6I GTAC 4 cut(s) 340, 376, 1782, 1980
CspCI CAANNNNNGTGG 2 cut(s) 1759, 1794
CviAII CATG 7 cut(s) 142, 1049, 1079, 1217, 1390, 1559, 1624
CviQI GTAC 4 cut(s) 340, 376, 1782, 1980
DraIII CACNNNGTG 1 cut(s) 866
DriI GACNNNNNGTC 2 cut(s) 81, 289
Eam1104I CTCTTC 5 cut(s) 366, 1734, 2070, 2226, 2309
Eam1105I GACNNNNNGTC 2 cut(s) 81, 289
EarI CTCTTC 5 cut(s) 366, 1734, 2070, 2226, 2309
EciI GGCGGA 3 cut(s) 23, 339, 647
Ecl136II GAGCTC 3 cut(s) 43, 298, 982
Eco130I CCWWGG 5 cut(s) 471, 1558, 1623, 1830, 2202
Eco24I GRGCYC 5 cut(s) 45, 117, 196, 300, 984
Eco31I GGTCTC 2 cut(s) 19, 399
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 3 cut(s) 83, 205, 238
Eco53kI GAGCTC 3 cut(s) 43, 298, 982
Eco57I CTGAAG 4 cut(s) 221, 1130, 1454, 1715
Eco88I CYCGRG 2 cut(s) 44, 309
EcoICRI GAGCTC 3 cut(s) 43, 298, 982
EcoO109I RGGNCCY 3 cut(s) 192, 205, 255
EcoRI GAATTC 1 cut(s) 1944
EcoRII CCWGG 3 cut(s) 9, 36, 1508
EcoRV GATATC 1 cut(s) 501
EcoT14I CCWWGG 5 cut(s) 471, 1558, 1623, 1830, 2202
EcoT22I ATGCAT 1 cut(s) 1682
EcoT38I GRGCYC 5 cut(s) 45, 117, 196, 300, 984
ErhI CCWWGG 5 cut(s) 471, 1558, 1623, 1830, 2202
Esp3I CGTCTC 1 cut(s) 1904
FaeI CATG 7 cut(s) 145, 1052, 1082, 1220, 1393, 1562, 1627
FalI AAGNNNNNCTT 4 cut(s) 561, 593, 1239, 1271
FaqI GGGAC 1 cut(s) 69
FatI CATG 7 cut(s) 141, 1048, 1078, 1216, 1389, 1558, 1623
FauI CCCGC 2 cut(s) 322, 1461
Fnu4HI GCNGC 4 cut(s) 250, 334, 1031, 2245
FokI GGATG 6 cut(s) 1177, 1367, 1652, 1670, 2030, 2370
FriOI GRGCYC 5 cut(s) 45, 117, 196, 300, 984
Fsp4HI GCNGC 4 cut(s) 250, 334, 1031, 2245
FspBI CTAG 2 cut(s) 809, 1067
FspI TGCGCA 1 cut(s) 1769
GlaI GCGC 3 cut(s) 124, 417, 1769
GluI GCNGC 4 cut(s) 250, 334, 1031, 2245
GsaI CCCAGC 3 cut(s) 199, 1164, 2084
GsuI CTGGAG 3 cut(s) 59, 284, 1019
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 8 cut(s) 115, 180, 194, 249, 257, 1089, 1412, 1585
HapII CCGG 1 cut(s) 1962
HgaI GACGC 3 cut(s) 155, 345, 1886
HhaI GCGC 3 cut(s) 125, 418, 1770
Hin1I GRCGYC 2 cut(s) 147, 356
Hin1II CATG 7 cut(s) 145, 1052, 1082, 1220, 1393, 1562, 1627
Hin6I GCGC 3 cut(s) 123, 416, 1768
HinP1I GCGC 3 cut(s) 123, 416, 1768
HincII GTYRAC 2 cut(s) 911, 2335
HindII GTYRAC 2 cut(s) 911, 2335
HindIII AAGCTT 2 cut(s) 154, 2306
HinfI GANTC 8 cut(s) 403, 449, 1146, 1505, 1531, 2063, 2111, 2340
HpaII CCGG 1 cut(s) 1962
HphI GGTGA 2 cut(s) 298, 452
Hpy166II GTNNAC 6 cut(s) 274, 306, 776, 911, 2138, 2335
Hpy8I GTNNAC 6 cut(s) 274, 306, 776, 911, 2138, 2335
HpyCH4III ACNGT 6 cut(s) 1263, 1553, 1618, 1936, 2013, 2058
HpyCH4IV ACGT 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 6 cut(s) 330, 342, 1315, 1390, 1911, 2204
HpySE526I ACGT 1 cut(s) 365
Hsp92I GRCGYC 2 cut(s) 147, 356
Hsp92II CATG 7 cut(s) 145, 1052, 1082, 1220, 1393, 1562, 1627
HspAI GCGC 3 cut(s) 123, 416, 1768
KpnI GGTACC 1 cut(s) 1983
LguI GCTCTTC 1 cut(s) 2070
LmnI GCTCC 8 cut(s) 40, 295, 303, 326, 748, 979, 1038, 1759
Lsp1109I GCAGC 3 cut(s) 345, 1042, 2256
LweI GCATC 5 cut(s) 205, 1147, 1630, 1689, 2088
MaeI CTAG 2 cut(s) 809, 1067
MaeII ACGT 1 cut(s) 365
MaeIII GTNAC 3 cut(s) 361, 1612, 1930
MbiI CCGCTC 1 cut(s) 329
MfeI CAATTG 1 cut(s) 707
MflI RGATCY 1 cut(s) 2151
MhlI GDGCHC 5 cut(s) 45, 117, 196, 300, 984
MlyI GAGTC 4 cut(s) 458, 1525, 2072, 2120
MmeI TCCRAC 3 cut(s) 159, 273, 2052
Mph1103I ATGCAT 1 cut(s) 1682
MroXI GAANNNNTTC 2 cut(s) 1140, 1254
MseI TTAA 4 cut(s) 1130, 1329, 2184, 2267
MslI CAYNNNNRTG 2 cut(s) 186, 1353
MspA1I CMGCKG 3 cut(s) 199, 280, 1905
MspI CCGG 1 cut(s) 1962
MspR9I CCNGG 3 cut(s) 11, 38, 1510
MunI CAATTG 1 cut(s) 707
Mva1269I GAATGC 1 cut(s) 1682
MvaI CCWGG 3 cut(s) 11, 38, 1510
MvnI CGCG 2 cut(s) 418, 1275
MwoI GCNNNNNNNGC 6 cut(s) 330, 342, 1315, 1390, 1911, 2204
NcoI CCATGG 2 cut(s) 1558, 1623
NlaIII CATG 7 cut(s) 145, 1052, 1082, 1220, 1393, 1562, 1627
NlaIV GGNNCC 5 cut(s) 85, 115, 193, 194, 1981
NmeAIII GCCGAG 2 cut(s) 144, 1115
NmuCI GTSAC 1 cut(s) 361
NruI TCGCGA 1 cut(s) 1275
NsbI TGCGCA 1 cut(s) 1769
NsiI ATGCAT 1 cut(s) 1682
NspI RCATGY 1 cut(s) 1220
NspV TTCGAA 1 cut(s) 1144
OliI CACNNNNGTG 1 cut(s) 186
PaeR7I CTCGAG 1 cut(s) 44
PciSI GCTCTTC 1 cut(s) 2070
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 1682
PdmI GAANNNNTTC 2 cut(s) 1140, 1254
PfeI GAWTC 4 cut(s) 403, 1146, 1505, 2340
PkrI GCNGC 4 cut(s) 251, 335, 1032, 2246
PleI GAGTC 4 cut(s) 457, 1525, 2071, 2119
PpsI GAGTC 4 cut(s) 457, 1525, 2071, 2119
PpuMI RGGWCCY 1 cut(s) 205
PshAI GACNNNNGTC 1 cut(s) 906
Psp124BI GAGCTC 3 cut(s) 45, 300, 984
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 3 cut(s) 9, 36, 1508
PspFI CCCAGC 3 cut(s) 195, 1160, 2080
PspGI CCWGG 3 cut(s) 9, 36, 1508
PspN4I GGNNCC 5 cut(s) 85, 115, 193, 194, 1981
PspOMI GGGCCC 2 cut(s) 113, 192
PspPPI RGGWCCY 1 cut(s) 205
PsuI RGATCY 1 cut(s) 2151
PvuII CAGCTG 3 cut(s) 199, 280, 1905
RruI TCGCGA 1 cut(s) 1275
RsaI GTAC 4 cut(s) 341, 377, 1783, 1981
RsaNI GTAC 4 cut(s) 340, 376, 1782, 1980
RseI CAYNNNNRTG 2 cut(s) 186, 1353
SacI GAGCTC 3 cut(s) 45, 300, 984
SapI GCTCTTC 1 cut(s) 2070
SaqAI TTAA 4 cut(s) 1130, 1329, 2184, 2267
SatI GCNGC 4 cut(s) 250, 334, 1031, 2245
SchI GAGTC 4 cut(s) 458, 1525, 2072, 2120
ScrFI CCNGG 3 cut(s) 11, 38, 1510
SduI GDGCHC 5 cut(s) 45, 117, 196, 300, 984
SfaNI GCATC 5 cut(s) 205, 1147, 1630, 1689, 2088
SfcI CTRYAG 2 cut(s) 1579, 2277
Sfr274I CTCGAG 1 cut(s) 44
SfuI TTCGAA 1 cut(s) 1144
SinI GGWCC 3 cut(s) 83, 205, 238
SlaI CTCGAG 1 cut(s) 44
SmiMI CAYNNNNRTG 2 cut(s) 186, 1353
SmlI CTYRAG 6 cut(s) 44, 491, 518, 1518, 2261, 2309
SmoI CTYRAG 6 cut(s) 44, 491, 518, 1518, 2261, 2309
SspI AATATT 1 cut(s) 1139
SspMI CTAG 2 cut(s) 809, 1067
SstI GAGCTC 3 cut(s) 45, 300, 984
StyD4I CCNGG 3 cut(s) 9, 36, 1508
StyI CCWWGG 5 cut(s) 471, 1558, 1623, 1830, 2202
TaaI ACNGT 6 cut(s) 1263, 1553, 1618, 1936, 2013, 2058
TaiI ACGT 1 cut(s) 368
TaqI TCGA 6 cut(s) 45, 213, 1144, 1527, 1797, 2363
TaqII GACCGA 1 cut(s) 71
TatI WGTACW 1 cut(s) 375
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 4 cut(s) 403, 1146, 1505, 2340
Tru1I TTAA 4 cut(s) 1130, 1329, 2184, 2267
Tru9I TTAA 4 cut(s) 1130, 1329, 2184, 2267
TscAI CASTG 3 cut(s) 487, 1990, 2018
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 3 cut(s) 333, 1030, 2244
Tsp45I GTSAC 1 cut(s) 361
TspGWI ACGGA 2 cut(s) 176, 2376
TspRI CASTG 3 cut(s) 487, 1990, 2018
VpaK11BI GGWCC 3 cut(s) 83, 205, 238
XapI RAATTY 6 cut(s) 421, 1240, 1336, 1402, 1631, 1944
XceI RCATGY 1 cut(s) 1220
XcmI CCANNNNNNNNNTGG 2 cut(s) 284, 1990
XhoI CTCGAG 1 cut(s) 44
XmnI GAANNNNTTC 2 cut(s) 1140, 1254
XspI CTAG 2 cut(s) 809, 1067
Zsp2I ATGCAT 1 cut(s) 1682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.