pycom10g18430

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012861
Physical Location & Seq
Forward (+)
21708942 .. 21711315
2374 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1275 bp
ATGTGCAGCTCAAAGGCCAAGGTGACCTTAGGGATTGAAGTCACACCCATTGTGGCTAGAATCAATGGCAGGCCAGTCCTTCAGCCTAATTGCAACCGGGTTCCTAGCCTAGACCGGCGTGGTTCGATTAAGAAAATATCGACACCACCACCACCTCCACTACCATTGCCAACTTCTTCTGCTAGTATTACTAGTCATAGAACTACCAACAAGGGAGCTTCATCTTTGTTAACACCTCCCATTTCTCCCAAGTCAAAGTCCCCAAGGCCACCAGCAATCAAGAGAGGAAATGACCCTAATGGCCTCAATTCGAGTTCCGAAAAAGTTGTGACACCAGGAGGAACCACAAGAGCTAAGATTTTGGAGAGGAAGAAGTCCAAGAGTTTCAAGAGCGCTAGCGTTGGTGTTGATGTTGATGCTGCTGATCATAATCATGGAGGTTTTGGTAATGGTGGATTCTCTGCTTCTAATATCGAGGCGTCGTTGAGTTATTCCTCTTCTTTGATCACCGAGGCACCTGGCAGCATTGCTGCTGGGAGGAGGGAACAGATGGTGCTTCAGCATGCACAACGGAAGATGAGGATTGCCCATTACGGCAGGTCAAAGTCTGCCAACTTTGAAGGGGTTGTTCCTGTTGATGCTTCTGGTAATGTAGAAGCCAAGGCAGCTGAAGAAGAGAAAAGGTGCAGCTTTATCACGGCTAATTCAGATCCCATCTATGTTGCTTACCATGATGAAGAATGGGGAGTTCCTGTCCATGATGACAAGATGTTGTTTGAACTGCTTGTGTTAACTGGAGCTCAAGTTGGCTCAGATTGGACTTCCATTTTGAAAAAACGTCAAGATTTCAGGGATGCATTTTCAGGATTTGATGCAGAAACTGTGGCAAATTTAAGTGATAAACAGATGATGTCAATTGCTTCAGAATATGGCATTGAAATCAGCAGGATCCGAGGGGTTGTCGACAACTCTAACAGAATTCTTGAGATTAAGAAGGAATTTGGATCATTTGACAAGTACATCTGGGGATTTGTCAATGAAAAACCCATCTCACCACAATATAAATTAGGCTACAAAATCCCAGTGAAGACATCCAAATCAGAGTCGATCAGCAGAGACATGGTTAGGAGGGGATTTAGGTTTGTGGGTCCAACAGTTGTTCATTCATTCATGCAAGCCTCAGGCCTCACGAACGACCATCTGATCACTTGCCACAGCCACCTCCGATGCACCTTATTGGCTGCCTGCCGTCCCACATTGGAAGAAATTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

425

Amino Acids

46.15

Weight (kDa)

9.51

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 234 - 406 2.6e-64 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12710
fragaria_vesca FvH4_3g13890
malus_domestica MD05G1240400.v1.1 MD10G1218500.v1.1
prunus_persica Prupe.4G125700_v2.0.a1
pyrus_communis pycom05g21640 pycom10g18430
rosa_chinensis RchiOBHm_Chr5g0023071
rosa_laevigata RLG00000032698
rosa_multiflora Rmu_co8421393.1_g000001
rosa_roxburghii Rroxscaffold_1G00056250
rosa_rugosa Rorug05G0077100
rosa_samantha Rh5AG163200 Rh5BG162500 Rh5CG177300 Rh5DG163700
rosa_wichuraiana Rw5G014720 Rw5G014990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 588
AccB1I GGYRCC 1 cut(s) 514
AccI GTMKAC 1 cut(s) 963
AclWI GGATC 4 cut(s) 704, 943, 956, 1012
AcsI RAATTY 4 cut(s) 889, 978, 998, 1266
AcuI CTGAAG 4 cut(s) 65, 542, 690, 906
AcyI GRCGYC 1 cut(s) 479
AfaI GTAC 1 cut(s) 1019
AfeI AGCGCT 1 cut(s) 394
AgsI TTSAA 6 cut(s) 38, 388, 620, 779, 832, 938
AhlI ACTAGT 1 cut(s) 191
AjnI CCWGG 2 cut(s) 334, 517
AluBI AGCT 6 cut(s) 9, 218, 353, 668, 690, 800
AluI AGCT 6 cut(s) 9, 218, 353, 668, 690, 800
Alw21I GWGCWC 1 cut(s) 802
Alw26I GTCTC 1 cut(s) 1110
AlwI GGATC 4 cut(s) 704, 943, 956, 1012
AlwNI CAGNNNCTG 1 cut(s) 881
Aor51HI AGCGCT 1 cut(s) 394
AoxI GGCC 5 cut(s) 15, 71, 266, 301, 1183
ApeKI GCWGC 7 cut(s) 6, 419, 522, 530, 665, 687, 1241
ApoI RAATTY 4 cut(s) 889, 978, 998, 1266
ArsI GACNNNNNNTTYG 2 cut(s) 811, 843
Asp700I GAANNNNTTC 1 cut(s) 1266
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 1 cut(s) 1148
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 3 cut(s) 34, 499, 1044
AsuNHI GCTAGC 1 cut(s) 395
AvaII GGWCC 1 cut(s) 1148
AxyI CCTNAGG 2 cut(s) 28, 1180
BamHI GGATCC 1 cut(s) 948
BanI GGYRCC 1 cut(s) 514
BanII GRGCYC 1 cut(s) 802
BbsI GAAGAC 1 cut(s) 1094
Bbv12I GWGCWC 1 cut(s) 802
BbvI GCAGC 7 cut(s) 18, 406, 517, 534, 677, 699, 1228
BccI CCATC 4 cut(s) 544, 722, 1055, 1206
BceAI ACGGC 3 cut(s) 610, 714, 1233
BciT130I CCWGG 2 cut(s) 336, 519
BclI TGATCA 3 cut(s) 424, 504, 1203
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 1110
BcuI ACTAGT 1 cut(s) 191
BfaI CTAG 6 cut(s) 57, 105, 110, 183, 192, 396
BfmI CTRYAG 1 cut(s) 1271
BfoI RGCGCY 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 588
BisI GCNGC 7 cut(s) 7, 420, 523, 531, 666, 688, 1242
BlsI GCNGC 7 cut(s) 8, 421, 524, 532, 667, 689, 1243
Bme1390I CCNGG 3 cut(s) 98, 336, 519
Bme18I GGWCC 1 cut(s) 1148
BmgT120I GGNCC 1 cut(s) 1148
BmiI GGNNCC 5 cut(s) 102, 343, 516, 950, 1149
BmrFI CCNGG 3 cut(s) 98, 336, 519
BmrI ACTGGG 1 cut(s) 1076
BmsI GCATC 5 cut(s) 406, 628, 844, 862, 1217
BmtI GCTAGC 1 cut(s) 399
BmuI ACTGGG 1 cut(s) 1076
BpiI GAAGAC 1 cut(s) 1094
BpmI CTGGAG 1 cut(s) 816
BpuEI CTTGAG 2 cut(s) 786, 1004
BpuMI CCSGG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 479
BsaJI CCNNGG 5 cut(s) 18, 263, 510, 660, 952
BsaXI ACNNNNNCTCC 4 cut(s) 738, 768, 789, 819
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 3 cut(s) 74, 799, 1082
Bse21I CCTNAGG 2 cut(s) 28, 1180
Bse3DI GCAATG 2 cut(s) 164, 525
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 2 cut(s) 336, 519
BseDI CCNNGG 5 cut(s) 18, 263, 510, 660, 952
BseGI GGATG 2 cut(s) 859, 1091
BseJI GATNNNNATC 1 cut(s) 429
BseMI GCAATG 2 cut(s) 164, 525
BseMII CTCAG 2 cut(s) 825, 1194
BseNI ACTGG 3 cut(s) 74, 799, 1082
BseRI GAGGAG 1 cut(s) 553
BseXI GCAGC 7 cut(s) 18, 406, 517, 534, 677, 699, 1228
BseYI CCCAGC 1 cut(s) 533
BsgI GTGCAG 2 cut(s) 25, 706
BshFI GGCC 5 cut(s) 17, 73, 268, 303, 1185
BshNI GGYRCC 1 cut(s) 514
BsiHKAI GWGCWC 1 cut(s) 802
BsiSI CCGG 2 cut(s) 97, 115
BslFI GGGAC 2 cut(s) 244, 1236
BsmAI GTCTC 1 cut(s) 1110
BsmFI GGGAC 2 cut(s) 244, 1236
BsnI GGCC 5 cut(s) 17, 73, 268, 303, 1185
Bsp1286I GDGCHC 1 cut(s) 802
Bsp143I GATC 7 cut(s) 424, 504, 709, 948, 1004, 1107, 1203
BspANI GGCC 5 cut(s) 17, 73, 268, 303, 1185
BspCNI CTCAG 2 cut(s) 824, 1193
BspLI GGNNCC 5 cut(s) 102, 343, 516, 950, 1149
BspMI ACCTGC 1 cut(s) 588
BspOI GCTAGC 1 cut(s) 399
BspPI GGATC 4 cut(s) 704, 943, 956, 1012
BspT107I GGYRCC 1 cut(s) 514
BsrDI GCAATG 2 cut(s) 164, 525
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 3 cut(s) 74, 799, 1082
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 5 cut(s) 18, 263, 510, 660, 952
BssMI GATC 7 cut(s) 424, 504, 709, 948, 1004, 1107, 1203
BssNI GRCGYC 1 cut(s) 479
BssT1I CCWWGG 3 cut(s) 18, 263, 660
Bst2UI CCWGG 2 cut(s) 336, 519
Bst4CI ACNGT 2 cut(s) 883, 1156
Bst6I CTCTTC 2 cut(s) 502, 669
BstACI GRCGYC 1 cut(s) 479
BstC8I GCNNGC 5 cut(s) 71, 397, 564, 1176, 1246
BstDEI CTNAG 4 cut(s) 28, 354, 811, 1180
BstEII GGTNACC 1 cut(s) 22
BstF5I GGATG 2 cut(s) 859, 1091
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 7 cut(s) 427, 507, 712, 951, 1007, 1110, 1206
BstMAI GTCTC 1 cut(s) 1110
BstMBI GATC 7 cut(s) 424, 504, 709, 948, 1004, 1107, 1203
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstNI CCWGG 2 cut(s) 336, 519
BstNSI RCATGY 1 cut(s) 566
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 3 cut(s) 96, 334, 517
BstSFI CTRYAG 1 cut(s) 1271
BstV1I GCAGC 7 cut(s) 18, 406, 517, 534, 677, 699, 1228
BstV2I GAAGAC 1 cut(s) 1094
BstX2I RGATCY 2 cut(s) 709, 948
BstYI RGATCY 2 cut(s) 709, 948
Bsu36I CCTNAGG 2 cut(s) 28, 1180
BsuRI GGCC 5 cut(s) 17, 73, 268, 303, 1185
BtsCI GGATG 2 cut(s) 859, 1091
BtsIMutI CAGTG 1 cut(s) 1089
BveI ACCTGC 1 cut(s) 588
Cac8I GCNNGC 5 cut(s) 71, 397, 564, 1176, 1246
CaiI CAGNNNCTG 1 cut(s) 881
CfoI GCGC 1 cut(s) 395
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 1 cut(s) 1148
CseI GACGC 1 cut(s) 468
Csp6I GTAC 1 cut(s) 1018
CspCI CAANNNNNGTGG 2 cut(s) 147, 182
CviAII CATG 6 cut(s) 434, 563, 731, 758, 1120, 1171
CviQI GTAC 1 cut(s) 1018
DdeI CTNAG 4 cut(s) 28, 354, 811, 1180
DpnI GATC 7 cut(s) 426, 506, 711, 950, 1006, 1109, 1205
DpnII GATC 7 cut(s) 424, 504, 709, 948, 1004, 1107, 1203
Eam1104I CTCTTC 2 cut(s) 502, 669
EarI CTCTTC 2 cut(s) 502, 669
Ecl136II GAGCTC 1 cut(s) 800
Eco130I CCWWGG 3 cut(s) 18, 263, 660
Eco147I AGGCCT 1 cut(s) 1185
Eco24I GRGCYC 1 cut(s) 802
Eco47I GGWCC 1 cut(s) 1148
Eco47III AGCGCT 1 cut(s) 394
Eco53kI GAGCTC 1 cut(s) 800
Eco57I CTGAAG 4 cut(s) 65, 542, 690, 906
Eco81I CCTNAGG 2 cut(s) 28, 1180
Eco91I GGTNACC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 800
EcoO65I GGTNACC 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 978
EcoRII CCWGG 2 cut(s) 334, 517
EcoT14I CCWWGG 3 cut(s) 18, 263, 660
EcoT22I ATGCAT 1 cut(s) 859
EcoT38I GRGCYC 1 cut(s) 802
ErhI CCWWGG 3 cut(s) 18, 263, 660
FaeI CATG 6 cut(s) 437, 566, 734, 761, 1123, 1174
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 2 cut(s) 244, 1236
FatI CATG 6 cut(s) 433, 562, 730, 757, 1119, 1170
FbaI TGATCA 3 cut(s) 424, 504, 1203
FblI GTMKAC 1 cut(s) 963
Fnu4HI GCNGC 7 cut(s) 7, 420, 523, 531, 666, 688, 1242
FokI GGATG 2 cut(s) 866, 1078
FriOI GRGCYC 1 cut(s) 802
Fsp4HI GCNGC 7 cut(s) 7, 420, 523, 531, 666, 688, 1242
FspBI CTAG 6 cut(s) 57, 105, 110, 183, 192, 396
GlaI GCGC 1 cut(s) 394
GluI GCNGC 7 cut(s) 7, 420, 523, 531, 666, 688, 1242
GsaI CCCAGC 1 cut(s) 537
GsuI CTGGAG 1 cut(s) 816
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 5 cut(s) 17, 73, 268, 303, 1185
HapII CCGG 2 cut(s) 97, 115
HgaI GACGC 1 cut(s) 468
HhaI GCGC 1 cut(s) 395
Hin1I GRCGYC 1 cut(s) 479
Hin1II CATG 6 cut(s) 437, 566, 734, 761, 1123, 1174
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HincII GTYRAC 3 cut(s) 231, 792, 964
HindII GTYRAC 3 cut(s) 231, 792, 964
HinfI GANTC 3 cut(s) 60, 456, 1103
HpaI GTTAAC 2 cut(s) 231, 792
HpaII CCGG 2 cut(s) 97, 115
HphI GGTGA 3 cut(s) 34, 499, 1044
Hpy166II GTNNAC 3 cut(s) 231, 792, 964
Hpy188I TCNGA 8 cut(s) 319, 709, 814, 925, 953, 1102, 1203, 1226
Hpy188III TCNNGA 6 cut(s) 280, 388, 842, 864, 983, 1189
Hpy8I GTNNAC 3 cut(s) 231, 792, 964
Hpy99I CGWCG 1 cut(s) 484
HpyAV CCTTC 3 cut(s) 89, 614, 988
HpyCH4III ACNGT 2 cut(s) 883, 1156
HpyCH4IV ACGT 1 cut(s) 838
HpyCH4V TGCA 8 cut(s) 6, 93, 566, 687, 857, 875, 1174, 1230
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 4 cut(s) 28, 354, 811, 1180
HpySE526I ACGT 1 cut(s) 838
Hsp92I GRCGYC 1 cut(s) 479
Hsp92II CATG 6 cut(s) 437, 566, 734, 761, 1123, 1174
HspAI GCGC 1 cut(s) 393
Ksp22I TGATCA 3 cut(s) 424, 504, 1203
KspAI GTTAAC 2 cut(s) 231, 792
Kzo9I GATC 7 cut(s) 424, 504, 709, 948, 1004, 1107, 1203
LmnI GCTCC 2 cut(s) 215, 797
Lsp1109I GCAGC 7 cut(s) 18, 406, 517, 534, 677, 699, 1228
LweI GCATC 5 cut(s) 406, 628, 844, 862, 1217
MaeI CTAG 6 cut(s) 57, 105, 110, 183, 192, 396
MaeII ACGT 1 cut(s) 838
MaeIII GTNAC 3 cut(s) 22, 40, 328
MalI GATC 7 cut(s) 426, 506, 711, 950, 1006, 1109, 1205
MboI GATC 7 cut(s) 424, 504, 709, 948, 1004, 1107, 1203
MboII GAAGA 9 cut(s) 168, 382, 489, 586, 683, 686, 749, 1099, 1274
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 2 cut(s) 709, 948
MhlI GDGCHC 1 cut(s) 802
MluCI AATT 9 cut(s) 88, 307, 703, 889, 915, 978, 998, 1064, 1266
MlyI GAGTC 1 cut(s) 1112
MmeI TCCRAC 1 cut(s) 1175
Mph1103I ATGCAT 1 cut(s) 859
MroXI GAANNNNTTC 1 cut(s) 1266
MseI TTAA 5 cut(s) 129, 230, 791, 893, 990
MslI CAYNNNNRTG 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 2 cut(s) 97, 115
MspR9I CCNGG 3 cut(s) 98, 336, 519
MunI CAATTG 1 cut(s) 915
MvaI CCWGG 2 cut(s) 336, 519
MwoI GCNNNNNNNGC 1 cut(s) 665
NciI CCSGG 1 cut(s) 98
NdeII GATC 7 cut(s) 424, 504, 709, 948, 1004, 1107, 1203
NheI GCTAGC 1 cut(s) 395
NlaIII CATG 6 cut(s) 437, 566, 734, 761, 1123, 1174
NlaIV GGNNCC 5 cut(s) 102, 343, 516, 950, 1149
NmuCI GTSAC 3 cut(s) 22, 40, 328
NsiI ATGCAT 1 cut(s) 859
NspI RCATGY 1 cut(s) 566
PaeI GCATGC 1 cut(s) 566
PceI AGGCCT 1 cut(s) 1185
PdmI GAANNNNTTC 1 cut(s) 1266
PfeI GAWTC 2 cut(s) 60, 456
PkrI GCNGC 7 cut(s) 8, 421, 524, 532, 667, 689, 1243
PleI GAGTC 1 cut(s) 1111
PpsI GAGTC 1 cut(s) 1111
Psp124BI GAGCTC 1 cut(s) 802
Psp6I CCWGG 2 cut(s) 334, 517
PspEI GGTNACC 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 533
PspGI CCWGG 2 cut(s) 334, 517
PspN4I GGNNCC 5 cut(s) 102, 343, 516, 950, 1149
PspPI GGNCC 1 cut(s) 1148
PstNI CAGNNNCTG 1 cut(s) 881
PsuI RGATCY 2 cut(s) 709, 948
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 1 cut(s) 1019
RsaNI GTAC 1 cut(s) 1018
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 1 cut(s) 802
SalI GTCGAC 1 cut(s) 962
SaqAI TTAA 5 cut(s) 129, 230, 791, 893, 990
SatI GCNGC 7 cut(s) 7, 420, 523, 531, 666, 688, 1242
Sau3AI GATC 7 cut(s) 424, 504, 709, 948, 1004, 1107, 1203
Sau96I GGNCC 1 cut(s) 1148
SchI GAGTC 1 cut(s) 1112
ScrFI CCNGG 3 cut(s) 98, 336, 519
SduI GDGCHC 1 cut(s) 802
SfaNI GCATC 5 cut(s) 406, 628, 844, 862, 1217
SfcI CTRYAG 1 cut(s) 1271
SinI GGWCC 1 cut(s) 1148
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 2 cut(s) 801, 983
SmoI CTYRAG 2 cut(s) 801, 983
SpeI ACTAGT 1 cut(s) 191
SphI GCATGC 1 cut(s) 566
Sse9I AATT 9 cut(s) 88, 307, 703, 889, 915, 978, 998, 1064, 1266
SseBI AGGCCT 1 cut(s) 1185
SspMI CTAG 6 cut(s) 57, 105, 110, 183, 192, 396
SstI GAGCTC 1 cut(s) 802
StuI AGGCCT 1 cut(s) 1185
StyD4I CCNGG 3 cut(s) 96, 334, 517
StyI CCWWGG 3 cut(s) 18, 263, 660
TaaI ACNGT 2 cut(s) 883, 1156
TaiI ACGT 1 cut(s) 841
TaqI TCGA 6 cut(s) 125, 140, 311, 474, 963, 1106
TasI AATT 9 cut(s) 88, 307, 703, 889, 915, 978, 998, 1064, 1266
TatI WGTACW 1 cut(s) 1017
TfiI GAWTC 2 cut(s) 60, 456
Tru1I TTAA 5 cut(s) 129, 230, 791, 893, 990
Tru9I TTAA 5 cut(s) 129, 230, 791, 893, 990
TscAI CASTG 1 cut(s) 1089
TseFI GTSAC 3 cut(s) 22, 40, 328
TseI GCWGC 7 cut(s) 6, 419, 522, 530, 665, 687, 1241
Tsp45I GTSAC 3 cut(s) 22, 40, 328
TspDTI ATGAA 6 cut(s) 210, 750, 1053, 1151, 1155, 1159
TspGWI ACGGA 1 cut(s) 586
TspRI CASTG 1 cut(s) 1089
VpaK11BI GGWCC 1 cut(s) 1148
XapI RAATTY 4 cut(s) 889, 978, 998, 1266
XceI RCATGY 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 963
XmnI GAANNNNTTC 1 cut(s) 1266
XspI CTAG 6 cut(s) 57, 105, 110, 183, 192, 396
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.