pycom10g19580

Glutathione S-transferase omega-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005011
Physical Location & Seq
Forward (+)
22635105 .. 22637576
2472 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
ATGATCTTTTCCTCGTTTTCAGACTTTTCTTTTTCTATTTGTTTATTGTTACCATTCAGATCTGGTCGGTCGGACGCAGAGCTCACCTCTCTCTCTCTCTCTCTCTCTCTCTCTTGCTCTCTCTCTCCGAGTCTCTCCCTCAGATCCCTTTCAAGCCTTCAAGGTATTCATTCTTCAGCACACAATCAAATGGCTCGATCTGCACTAGACGAGACCTCACTATCAGGTGCATTTGTGAGAACTGCTTCTGTATTTCGTAACTTCATTTCGCGGGACCCGAATTCTCAATTTCCGGCAGAAGCTGGAAGATATCATCTGTATGTATCATATGCTTGCCCTTGGGCTTCCAGGTGCCTTGCGTACTTGAACATAAAAGGACTCGATAAAGCCATCAGCTTTACGTCAGTCAAACCGATATGGGAAAGGACAAAAGAAAGTGATGAACATATGGGGTGGGTTTTTCCTGCTTCTGAAACAGAGCTAGCGGGAGCTGAACCTGACCCTTTGAATGGAGCAAAAAGCATTAGAGAACTTTACAAGCTTGCAAGCACACAGTATACTGGAAAGTACACAGTTCCAGTTCTATGGGATAAAAAACTCAAAACAATTGTGAGTAATGAGAGCGGGGAGATTATTCGCATGTTCAATACTGAATTCAACGATATAGCTGAAAATGCGTCTTTGGACTTATATCCTCCTCACTTGCAATCCCAAATTGATCAGACAAATGAGTGGATATATGACAAGATAAACAACGGTGTCTATAAATGCGGGTTTGCGAGGAAGCAAGAGCCTTATGATGAGGCTGTGAAACAATTGTACGAAGCTTTGGATAAATGTGAGGAGATACTTAGCAAGCAACGATACCTGTGCGGGAACACATTGTCTGAAGCAGATATTCGGTTGTTTGTCACTCTTATAAGATTCGACGAGGTTTACGTAGTCCACTTCAAGTGCAACAAGAAAGTACTACGAGAATACCCGAATTTGTTCAACTACACCAAAGACATTTTCCAGGTTCCGGGGATGAGCAGCACAGTCAACATGGATCACATAAAGCGGCACTACTACGGAAGCCATCCGTCTATCAATCCATTCGGAATAATTCCTAGCGGGCCAGATATCGACTACTCTTCGCCTCATGACAGAAACAGGTTTTCGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

389

Amino Acids

44.02

Weight (kDa)

6.38

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_2 PF13409 111 - 217 6.5e-09 Glutathione S-transferase, N-terminal domain
GST_C PF00043 259 - 339 7.3e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 265 - 334 5.9e-14 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 270 - 342 5.5e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 920
AccB1I GGYRCC 1 cut(s) 351
AccBSI CCGCTC 1 cut(s) 624
AccI GTMKAC 1 cut(s) 557
AccII CGCG 1 cut(s) 271
AciI CCGC 7 cut(s) 271, 485, 624, 771, 873, 1060, 1113
AclWI GGATC 2 cut(s) 138, 1056
AcsI RAATTY 3 cut(s) 280, 653, 985
AcuI CTGAAG 2 cut(s) 159, 909
AfaI GTAC 4 cut(s) 362, 569, 821, 969
AfiI CCNNNNNNNGG 2 cut(s) 509, 1021
AgsI TTSAA 8 cut(s) 153, 161, 367, 508, 646, 658, 952, 994
AjnI CCWGG 2 cut(s) 347, 1014
AjuI GAANNNNNNNTTGG 2 cut(s) 995, 1027
AluBI AGCT 8 cut(s) 82, 302, 396, 481, 491, 541, 668, 827
AluI AGCT 8 cut(s) 82, 302, 396, 481, 491, 541, 668, 827
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 2 cut(s) 137, 206
AlwI GGATC 2 cut(s) 138, 1056
AlwNI CAGNNNCTG 1 cut(s) 302
AoxI GGCC 1 cut(s) 1115
ApeKI GCWGC 1 cut(s) 1032
ApoI RAATTY 3 cut(s) 280, 653, 985
Asp700I GAANNNNTTC 2 cut(s) 244, 989
AspS9I GGNCC 2 cut(s) 274, 1115
AsuC2I CCSGG 1 cut(s) 1023
AsuHPI GGTGA 1 cut(s) 76
AsuNHI GCTAGC 1 cut(s) 481
AvaII GGWCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 351
BanII GRGCYC 1 cut(s) 84
BarI GAAGNNNNNNTAC 2 cut(s) 157, 189
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 1044
BccI CCATC 2 cut(s) 398, 1086
BcgI CGANNNNNNTGC 2 cut(s) 852, 886
BciT130I CCWGG 2 cut(s) 349, 1016
BclI TGATCA 1 cut(s) 718
BcnI CCSGG 1 cut(s) 1023
BcoDI GTCTC 2 cut(s) 137, 206
BfaI CTAG 3 cut(s) 206, 482, 1110
BglII AGATCT 1 cut(s) 59
BisI GCNGC 2 cut(s) 1033, 1061
BlsI GCNGC 2 cut(s) 1034, 1062
BmcAI AGTACT 1 cut(s) 969
Bme1390I CCNGG 3 cut(s) 349, 1016, 1023
Bme18I GGWCC 1 cut(s) 274
BmgT120I GGNCC 2 cut(s) 274, 1115
BmiI GGNNCC 4 cut(s) 275, 276, 353, 1020
BmrFI CCNGG 3 cut(s) 349, 1016, 1023
BmtI GCTAGC 1 cut(s) 485
BplI GAGNNNNNCTC 2 cut(s) 71, 103
BpuMI CCSGG 1 cut(s) 1023
BsaAI YACGTR 1 cut(s) 940
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 2 cut(s) 338, 1022
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 1021
Bse1I ACTGG 2 cut(s) 565, 578
BseBI CCWGG 2 cut(s) 349, 1016
BseDI CCNNGG 2 cut(s) 338, 1022
BseGI GGATG 2 cut(s) 1032, 1078
BseLI CCNNNNNNNGG 2 cut(s) 509, 1021
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 2 cut(s) 565, 578
BseRI GAGGAG 2 cut(s) 687, 857
BseXI GCAGC 1 cut(s) 1044
BsgI GTGCAG 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 271
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 1 cut(s) 1117
BshNI GGYRCC 1 cut(s) 351
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 2 cut(s) 293, 1022
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 2 cut(s) 509, 1021
BsmAI GTCTC 2 cut(s) 137, 206
BsmFI GGGAC 1 cut(s) 287
BsnI GGCC 1 cut(s) 1117
Bso31I GGTCTC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 6 cut(s) 3, 59, 143, 197, 718, 1048
BspACI CCGC 7 cut(s) 271, 485, 624, 771, 873, 1060, 1113
BspANI GGCC 1 cut(s) 1117
BspCNI CTCAG 1 cut(s) 153
BspFNI CGCG 1 cut(s) 271
BspHI TCATGA 1 cut(s) 1141
BspLI GGNNCC 4 cut(s) 275, 276, 353, 1020
BspOI GCTAGC 1 cut(s) 485
BspPI GGATC 2 cut(s) 138, 1056
BspT107I GGYRCC 1 cut(s) 351
BspTNI GGTCTC 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 624
BsrI ACTGG 2 cut(s) 565, 578
BssECI CCNNGG 2 cut(s) 338, 1022
BssMI GATC 6 cut(s) 3, 59, 143, 197, 718, 1048
BssNAI GTATAC 1 cut(s) 558
BssT1I CCWWGG 1 cut(s) 338
Bst1107I GTATAC 1 cut(s) 558
Bst2UI CCWGG 2 cut(s) 349, 1016
Bst4CI ACNGT 4 cut(s) 555, 574, 758, 1039
Bst6I CTCTTC 1 cut(s) 1138
BstBAI YACGTR 1 cut(s) 940
BstC8I GCNNGC 6 cut(s) 334, 483, 543, 547, 857, 1115
BstDEI CTNAG 3 cut(s) 140, 851, 1164
BstF5I GGATG 2 cut(s) 1032, 1078
BstFNI CGCG 1 cut(s) 271
BstKTI GATC 6 cut(s) 6, 62, 146, 200, 721, 1051
BstMAI GTCTC 2 cut(s) 137, 206
BstMBI GATC 6 cut(s) 3, 59, 143, 197, 718, 1048
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 2 cut(s) 200, 674
BstNI CCWGG 2 cut(s) 349, 1016
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 3 cut(s) 347, 1014, 1021
BstSNI TACGTA 1 cut(s) 940
BstUI CGCG 1 cut(s) 271
BstV1I GCAGC 1 cut(s) 1044
BstX2I RGATCY 2 cut(s) 59, 143
BstXI CCANNNNNNTGG 1 cut(s) 585
BstYI RGATCY 2 cut(s) 59, 143
BstZ17I GTATAC 1 cut(s) 558
BsuRI GGCC 1 cut(s) 1117
BtsCI GGATG 2 cut(s) 1032, 1078
Cac8I GCNNGC 6 cut(s) 334, 483, 543, 547, 857, 1115
CaiI CAGNNNCTG 1 cut(s) 302
CciI TCATGA 1 cut(s) 1141
Cfr13I GGNCC 2 cut(s) 274, 1115
CseI GACGC 2 cut(s) 83, 666
Csp6I GTAC 4 cut(s) 361, 568, 820, 968
CviAII CATG 3 cut(s) 640, 1045, 1142
CviQI GTAC 4 cut(s) 361, 568, 820, 968
DdeI CTNAG 3 cut(s) 140, 851, 1164
DpnI GATC 6 cut(s) 5, 61, 145, 199, 720, 1050
DpnII GATC 6 cut(s) 3, 59, 143, 197, 718, 1048
Eam1104I CTCTTC 1 cut(s) 1138
EarI CTCTTC 1 cut(s) 1138
Ecl136II GAGCTC 1 cut(s) 82
Eco105I TACGTA 1 cut(s) 940
Eco130I CCWWGG 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 84
Eco31I GGTCTC 1 cut(s) 206
Eco32I GATATC 2 cut(s) 311, 1123
Eco47I GGWCC 1 cut(s) 274
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 2 cut(s) 159, 909
EcoICRI GAGCTC 1 cut(s) 82
EcoO109I RGGNCCY 1 cut(s) 274
EcoRI GAATTC 2 cut(s) 280, 653
EcoRII CCWGG 2 cut(s) 347, 1014
EcoRV GATATC 2 cut(s) 311, 1123
EcoT14I CCWWGG 1 cut(s) 338
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 3 cut(s) 643, 1048, 1145
FaqI GGGAC 1 cut(s) 287
FatI CATG 3 cut(s) 639, 1044, 1141
FauI CCCGC 6 cut(s) 264, 478, 617, 764, 866, 1106
FauNDI CATATG 2 cut(s) 328, 447
FbaI TGATCA 1 cut(s) 718
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 2 cut(s) 1033, 1061
FokI GGATG 2 cut(s) 1039, 1065
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 2 cut(s) 1033, 1061
FspBI CTAG 3 cut(s) 206, 482, 1110
GluI GCNGC 2 cut(s) 1033, 1061
HaeIII GGCC 1 cut(s) 1117
HapII CCGG 2 cut(s) 293, 1022
HgaI GACGC 2 cut(s) 83, 666
Hin1II CATG 3 cut(s) 643, 1048, 1145
HincII GTYRAC 1 cut(s) 1042
HindII GTYRAC 1 cut(s) 1042
HindIII AAGCTT 2 cut(s) 539, 825
HinfI GANTC 3 cut(s) 130, 378, 924
HpaII CCGG 2 cut(s) 293, 1022
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 5 cut(s) 558, 570, 937, 946, 1042
Hpy188I TCNGA 9 cut(s) 22, 59, 73, 129, 143, 472, 723, 889, 1100
Hpy188III TCNNGA 1 cut(s) 1142
Hpy8I GTNNAC 5 cut(s) 558, 570, 937, 946, 1042
Hpy99I CGWCG 1 cut(s) 932
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 4 cut(s) 555, 574, 758, 1039
HpyCH4IV ACGT 2 cut(s) 401, 939
HpyCH4V TGCA 5 cut(s) 203, 230, 545, 706, 957
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 674
HpyF3I CTNAG 3 cut(s) 140, 851, 1164
HpySE526I ACGT 2 cut(s) 401, 939
Hsp92II CATG 3 cut(s) 643, 1048, 1145
KflI GGGWCCC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 6 cut(s) 3, 59, 143, 197, 718, 1048
LmnI GCTCC 2 cut(s) 488, 512
Lsp1109I GCAGC 1 cut(s) 1044
MaeI CTAG 3 cut(s) 206, 482, 1110
MaeII ACGT 2 cut(s) 401, 939
MaeIII GTNAC 3 cut(s) 48, 257, 910
MalI GATC 6 cut(s) 5, 61, 145, 199, 720, 1050
MbiI CCGCTC 1 cut(s) 624
MboI GATC 6 cut(s) 3, 59, 143, 197, 718, 1048
MboII GAAGA 3 cut(s) 165, 318, 1125
MfeI CAATTG 2 cut(s) 606, 815
MflI RGATCY 2 cut(s) 59, 143
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 8 cut(s) 280, 287, 606, 653, 714, 815, 985, 1104
MlyI GAGTC 2 cut(s) 139, 372
MmeI TCCRAC 1 cut(s) 51
MroXI GAANNNNTTC 2 cut(s) 244, 989
MslI CAYNNNNRTG 1 cut(s) 318
MspI CCGG 2 cut(s) 293, 1022
MspR9I CCNGG 3 cut(s) 349, 1016, 1023
MunI CAATTG 2 cut(s) 606, 815
MvaI CCWGG 2 cut(s) 349, 1016
MvnI CGCG 1 cut(s) 271
MwoI GCNNNNNNNGC 2 cut(s) 200, 674
NciI CCSGG 1 cut(s) 1023
NdeI CATATG 2 cut(s) 328, 447
NdeII GATC 6 cut(s) 3, 59, 143, 197, 718, 1048
NheI GCTAGC 1 cut(s) 481
NlaIII CATG 3 cut(s) 643, 1048, 1145
NlaIV GGNNCC 4 cut(s) 275, 276, 353, 1020
NmuCI GTSAC 1 cut(s) 910
NspI RCATGY 1 cut(s) 643
PagI TCATGA 1 cut(s) 1141
PcsI WCGNNNNNNNCGW 2 cut(s) 275, 936
PdmI GAANNNNTTC 2 cut(s) 244, 989
PfeI GAWTC 1 cut(s) 924
PkrI GCNGC 2 cut(s) 1034, 1062
PleI GAGTC 2 cut(s) 138, 372
PpsI GAGTC 2 cut(s) 138, 372
Ppu21I YACGTR 1 cut(s) 940
PpuMI RGGWCCY 1 cut(s) 274
PsiI TTATAA 1 cut(s) 920
Psp124BI GAGCTC 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 2 cut(s) 347, 1014
PspGI CCWGG 2 cut(s) 347, 1014
PspN4I GGNNCC 4 cut(s) 275, 276, 353, 1020
PspPI GGNCC 2 cut(s) 274, 1115
PspPPI RGGWCCY 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 2 cut(s) 59, 143
RsaI GTAC 4 cut(s) 362, 569, 821, 969
RsaNI GTAC 4 cut(s) 361, 568, 820, 968
RseI CAYNNNNRTG 1 cut(s) 318
SacI GAGCTC 1 cut(s) 84
SatI GCNGC 2 cut(s) 1033, 1061
Sau3AI GATC 6 cut(s) 3, 59, 143, 197, 718, 1048
Sau96I GGNCC 2 cut(s) 274, 1115
ScaI AGTACT 1 cut(s) 969
SchI GAGTC 2 cut(s) 139, 372
ScrFI CCNGG 3 cut(s) 349, 1016, 1023
SduI GDGCHC 1 cut(s) 84
SinI GGWCC 1 cut(s) 274
SmiMI CAYNNNNRTG 1 cut(s) 318
SnaBI TACGTA 1 cut(s) 940
Sse9I AATT 8 cut(s) 280, 287, 606, 653, 714, 815, 985, 1104
SsiI CCGC 7 cut(s) 271, 485, 624, 771, 873, 1060, 1113
SspMI CTAG 3 cut(s) 206, 482, 1110
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 3 cut(s) 347, 1014, 1021
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 4 cut(s) 555, 574, 758, 1039
TaiI ACGT 2 cut(s) 404, 942
TaqI TCGA 5 cut(s) 196, 381, 927, 1125, 1160
TaqII GACCGA 1 cut(s) 57
TasI AATT 8 cut(s) 280, 287, 606, 653, 714, 815, 985, 1104
TatI WGTACW 2 cut(s) 567, 967
TauI GCSGC 1 cut(s) 1063
TfiI GAWTC 1 cut(s) 924
TseFI GTSAC 1 cut(s) 910
TseI GCWGC 1 cut(s) 1032
Tsp45I GTSAC 1 cut(s) 910
TspDTI ATGAA 3 cut(s) 158, 253, 456
TspGWI ACGGA 2 cut(s) 1071, 1086
VpaK11BI GGWCC 1 cut(s) 274
XapI RAATTY 3 cut(s) 280, 653, 985
XceI RCATGY 1 cut(s) 643
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 2 cut(s) 244, 989
XspI CTAG 3 cut(s) 206, 482, 1110
ZrmI AGTACT 1 cut(s) 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.