pycom10g22270

Coatomer subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008972
Physical Location & Seq
Forward (+)
24678935 .. 24681109
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGGAGTCCTGCCCTTCGATAAAGAACATCCTCCTCCTCGATTCTGAAGGGAAACGTGTGGCTGTCAAGTATTATTCAGAGGAGGACTGGCCAACGAATACTGCCAAAGAATCTTTTGAGAAAGCTGTATTTACTAAAACTCAAAAGACAAATGCGCGGACAGAAGCTGAGATAGCAATGTTCGAGGGCACCATTGTAGTTTACAAGTTTGTTCAAGACCTTCACTTTTTTGTTACTGGTGGTGAAAATGAAAACGAGCTCATTTTAGCCACAGTTCTGCAGGGATTTTTTGATGCGGTTGGCATTCTCCTGAGAGGCAATGTGGACAAGAAGGAGGCACTTGAGAATTTGGATCTCATTTTACTATGCCTTGATGAAATTGTGGACGGAGGTATTATTCTCGAGACCGATTCAAATGTTATAGCAAGTAAGGTTGCAAGTCACAGTATTGATGCTGGAGCTCCGTTGTCTGAGCAGACAATAAGTCAAGCTTTGGCAACTGCCCGCGAACATCTAGCCAGACAACTTCTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

179

Amino Acids

19.6

Weight (kDa)

4.72

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 7 - 147 8.5e-23 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AccII CGCG 2 cut(s) 157, 507
AciI CCGC 3 cut(s) 157, 296, 505
AclWI GGATC 1 cut(s) 360
AcoI YGGCCR 1 cut(s) 89
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 66
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 2 cut(s) 215, 414
AhdI GACNNNNNGTC 1 cut(s) 483
AluBI AGCT 5 cut(s) 125, 167, 259, 461, 491
AluI AGCT 5 cut(s) 125, 167, 259, 461, 491
Alw21I GWGCWC 2 cut(s) 261, 463
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 1 cut(s) 360
AlwNI CAGNNNCTG 1 cut(s) 167
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 1 cut(s) 89
ApoI RAATTY 1 cut(s) 346
AspLEI GCGC 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 254
AvaI CYCGRG 1 cut(s) 401
BaeGI GKGCMC 1 cut(s) 191
BalI TGGCCA 1 cut(s) 91
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 2 cut(s) 261, 463
Bbv12I GWGCWC 2 cut(s) 261, 463
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 1 cut(s) 515
BfmI CTRYAG 1 cut(s) 278
BmeRI GACNNNNNGTC 1 cut(s) 483
BmeT110I CYCGRG 1 cut(s) 401
BmiI GGNNCC 1 cut(s) 190
BmsI GCATC 2 cut(s) 283, 442
BpmI CTGGAG 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 362
BsaI GGTCTC 1 cut(s) 398
Bse1I ACTGG 2 cut(s) 92, 241
Bse3DI GCAATG 2 cut(s) 183, 325
BseGI GGATG 1 cut(s) 27
BseMI GCAATG 2 cut(s) 183, 325
BseMII CTCAG 3 cut(s) 159, 302, 462
BseNI ACTGG 2 cut(s) 92, 241
BseRI GAGGAG 3 cut(s) 23, 26, 95
BseSI GKGCMC 1 cut(s) 191
Bsh1236I CGCG 2 cut(s) 157, 507
BshFI GGCC 1 cut(s) 91
BshNI GGYRCC 1 cut(s) 188
BsiHKAI GWGCWC 2 cut(s) 261, 463
BsiHKCI CYCGRG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 398
BsmI GAATGC 1 cut(s) 303
BsnI GGCC 1 cut(s) 91
Bso31I GGTCTC 1 cut(s) 398
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 3 cut(s) 191, 261, 463
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 3 cut(s) 157, 296, 505
BspANI GGCC 1 cut(s) 91
BspCNI CTCAG 3 cut(s) 160, 303, 463
BspFNI CGCG 2 cut(s) 157, 507
BspLI GGNNCC 1 cut(s) 190
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 398
BsrDI GCAATG 2 cut(s) 183, 325
BsrI ACTGG 2 cut(s) 92, 241
BssMI GATC 1 cut(s) 352
Bst4CI ACNGT 2 cut(s) 274, 446
BstC8I GCNNGC 1 cut(s) 505
BstDEI CTNAG 3 cut(s) 168, 311, 471
BstF5I GGATG 1 cut(s) 27
BstFNI CGCG 2 cut(s) 157, 507
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstSFI CTRYAG 1 cut(s) 278
BstSLI GKGCMC 1 cut(s) 191
BstUI CGCG 2 cut(s) 157, 507
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 1 cut(s) 91
BtsCI GGATG 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 505
CaiI CAGNNNCTG 1 cut(s) 167
CfoI GCGC 1 cut(s) 157
CviJI RGCY 9 cut(s) 62, 91, 125, 167, 259, 269, 461, 491, 518
CviKI_1 RGCY 9 cut(s) 62, 91, 125, 167, 259, 269, 461, 491, 518
DdeI CTNAG 3 cut(s) 168, 311, 471
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
DriI GACNNNNNGTC 1 cut(s) 483
EaeI YGGCCR 1 cut(s) 89
Eam1105I GACNNNNNGTC 1 cut(s) 483
Ecl136II GAGCTC 2 cut(s) 259, 461
Eco24I GRGCYC 2 cut(s) 261, 463
Eco31I GGTCTC 1 cut(s) 398
Eco53kI GAGCTC 2 cut(s) 259, 461
Eco57I CTGAAG 1 cut(s) 66
Eco88I CYCGRG 1 cut(s) 401
EcoICRI GAGCTC 2 cut(s) 259, 461
EcoT38I GRGCYC 2 cut(s) 261, 463
FaiI YATR 2 cut(s) 367, 422
FalI AAGNNNNNCTT 2 cut(s) 475, 507
FauI CCCGC 1 cut(s) 512
FokI GGATG 1 cut(s) 14
FriOI GRGCYC 2 cut(s) 261, 463
FspBI CTAG 1 cut(s) 515
GlaI GCGC 1 cut(s) 156
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 1 cut(s) 91
HhaI GCGC 1 cut(s) 157
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HindIII AAGCTT 1 cut(s) 489
HinfI GANTC 4 cut(s) 5, 41, 110, 410
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 3 cut(s) 202, 325, 385
Hpy188I TCNGA 3 cut(s) 46, 79, 472
Hpy188III TCNNGA 4 cut(s) 215, 310, 401, 403
Hpy8I GTNNAC 3 cut(s) 202, 325, 385
HpyAV CCTTC 4 cut(s) 24, 41, 230, 325
HpyCH4III ACNGT 2 cut(s) 274, 446
HpyCH4IV ACGT 1 cut(s) 55
HpyCH4V TGCA 2 cut(s) 280, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 3 cut(s) 168, 311, 471
HpySE526I ACGT 1 cut(s) 55
HspAI GCGC 1 cut(s) 155
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 2 cut(s) 458, 466
LpnPI CCDG 7 cut(s) 22, 73, 222, 266, 323, 441, 532
LweI GCATC 2 cut(s) 283, 442
MaeI CTAG 1 cut(s) 515
MaeII ACGT 1 cut(s) 55
MaeIII GTNAC 2 cut(s) 232, 440
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 3 cut(s) 191, 261, 463
MlsI TGGCCA 1 cut(s) 91
MluCI AATT 2 cut(s) 346, 378
MluNI TGGCCA 1 cut(s) 91
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 9 cut(s) 41, 44, 47, 73, 76, 178, 308, 328, 383
Mox20I TGGCCA 1 cut(s) 91
MscI TGGCCA 1 cut(s) 91
MseI TTAA 1 cut(s) 531
Msp20I TGGCCA 1 cut(s) 91
Mva1269I GAATGC 1 cut(s) 303
MvnI CGCG 2 cut(s) 157, 507
MwoI GCNNNNNNNGC 1 cut(s) 173
NdeII GATC 1 cut(s) 352
NlaIV GGNNCC 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 440
PaeR7I CTCGAG 1 cut(s) 401
PctI GAATGC 1 cut(s) 303
PfeI GAWTC 3 cut(s) 41, 110, 410
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 2 cut(s) 261, 463
PspN4I GGNNCC 1 cut(s) 190
PstI CTGCAG 1 cut(s) 282
PstNI CAGNNNCTG 1 cut(s) 167
PsuI RGATCY 1 cut(s) 352
SacI GAGCTC 2 cut(s) 261, 463
SaqAI TTAA 1 cut(s) 531
Sau3AI GATC 1 cut(s) 352
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 3 cut(s) 191, 261, 463
SetI ASST 9 cut(s) 58, 127, 169, 222, 261, 394, 435, 463, 493
SfaNI GCATC 2 cut(s) 283, 442
SfcI CTRYAG 1 cut(s) 278
Sfr274I CTCGAG 1 cut(s) 401
SlaI CTCGAG 1 cut(s) 401
SmlI CTYRAG 2 cut(s) 341, 401
SmoI CTYRAG 2 cut(s) 341, 401
Sse9I AATT 2 cut(s) 346, 378
SsiI CCGC 3 cut(s) 157, 296, 505
SspMI CTAG 1 cut(s) 515
SstI GAGCTC 2 cut(s) 261, 463
TaaI ACNGT 2 cut(s) 274, 446
TaiI ACGT 1 cut(s) 58
TaqI TCGA 4 cut(s) 17, 39, 183, 402
TaqII GACCGA 1 cut(s) 422
TasI AATT 2 cut(s) 346, 378
TfiI GAWTC 3 cut(s) 41, 110, 410
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TseFI GTSAC 1 cut(s) 440
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 264, 390
TspGWI ACGGA 2 cut(s) 402, 453
XapI RAATTY 1 cut(s) 346
XhoI CTCGAG 1 cut(s) 401
XspI CTAG 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.