pycom10g22730

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003665
Physical Location & Seq
Reverse (-)
25050108 .. 25051396
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 939 bp
ATGATCGATCCTCTTGAGCAGAGTCATCCCAAGTGGGCCGAAACTGTACAACTAGCACCAGATGTGATTAACATGACATTTACACCCATTGTCTCTTTGCTTGAAGGAGTGCCTGGAATAAAGCACTTGAGTCGCGCGATTGAGTTATATTTAGAGTACAAGCCACCAATTGAAGATCTGCAGTATTTCTTGGATTTTCAAATTGCTCAAGTTTGGGCCCCGGAGCATAATAATCTTCAAAGAAAAGAACCTGTGTGTACATCACTTCAGTTCAGCTTGATGGGACCGAAGCTTTACATCAGTCCGGATCAGGTAACAGTAGGCCGTAAGCCTGTCACAGGGCTTAGACTAAATCTAGAAGGCAGCAAACAGAACCGACTGGCTATACATTTGCAACATCTAGTGTCCCTTCCAAAAATTCTTCAACCCCATTGGGATGCGCACATGGCAATAGGTGCACCCAAGTGGCAAGGTCCGGAGGAGCAAGACAGCCGTTGGTTTGAACCAATCAAGTGGAAAAACTTCTCCCATGTAAGCACTGCACCGATAGAGCACGTGGAGGCTTCTATTGGTGACCTCTCCGGTGTTCACATTGTCACGGGGGCTCAGCTTGGTGTTTGGGATTTTGGTGCCAAAAATGTATTGCACCTCAAACTCCTCTTCTCCAAAGTACCAGGCTGCACTATCAGGCGTTCTGTTTGGGATCACAGCCCAGCCAGCCTGTCTTCTGCACAGAGGACGGATGGTTCTTCTTCATCGCATTTGAACAACAGAAGCTCTGACGATAAAAAGGACAAACTAGCAAAACTAGTGGACTTGACAGAAATGTCAAAGGGACCGCAAGATATACCAGGTCATTGGTTAGTCACCGGGGCTAAGCTTGGAGTCGACAAGGGAAAGATTATATTGCGCGTAAAATATTCCTTGCTGAACTACTGA

Protein Analysis

313

Amino Acids

34.95

Weight (kDa)

8.39

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 6 - 50 4.2e-08 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 826
Acc16I TGCGCA 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 629
AccI GTMKAC 1 cut(s) 888
AccII CGCG 3 cut(s) 135, 137, 912
AccIII TCCGGA 2 cut(s) 304, 475
AciI CCGC 1 cut(s) 839
AclWI GGATC 3 cut(s) 2, 315, 711
AcsI RAATTY 1 cut(s) 417
AcuI CTGAAG 1 cut(s) 251
AcvI CACGTG 1 cut(s) 556
AfaI GTAC 4 cut(s) 48, 158, 259, 672
AfiI CCNNNNNNNGG 1 cut(s) 338
AgsI TTSAA 7 cut(s) 104, 173, 200, 239, 425, 503, 766
AhlI ACTAGT 1 cut(s) 808
AjnI CCWGG 3 cut(s) 112, 673, 850
AleI CACNNNNGTG 1 cut(s) 463
AloI GAACNNNNNNTCC 2 cut(s) 730, 762
AluBI AGCT 5 cut(s) 276, 292, 610, 777, 880
AluI AGCT 5 cut(s) 276, 292, 610, 777, 880
Alw21I GWGCWC 2 cut(s) 460, 555
Alw26I GTCTC 1 cut(s) 97
Alw44I GTGCAC 1 cut(s) 456
AlwI GGATC 3 cut(s) 2, 315, 711
Aor13HI TCCGGA 2 cut(s) 304, 475
AoxI GGCC 3 cut(s) 36, 216, 322
ApaI GGGCCC 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 456
ApeKI GCWGC 2 cut(s) 363, 678
ApoI RAATTY 1 cut(s) 417
Asp700I GAANNNNTTC 1 cut(s) 521
AspLEI GCGC 3 cut(s) 137, 442, 912
AspS9I GGNCC 6 cut(s) 36, 216, 217, 284, 473, 836
AsuC2I CCSGG 2 cut(s) 221, 871
AsuHPI GGTGA 2 cut(s) 584, 859
AvaII GGWCC 3 cut(s) 284, 473, 836
BaeGI GKGCMC 2 cut(s) 220, 460
BanI GGYRCC 1 cut(s) 629
BanII GRGCYC 2 cut(s) 220, 607
BbrPI CACGTG 1 cut(s) 556
BbsI GAAGAC 1 cut(s) 717
Bbv12I GWGCWC 2 cut(s) 460, 555
BbvI GCAGC 2 cut(s) 375, 665
BccI CCATC 2 cut(s) 274, 737
BceAI ACGGC 2 cut(s) 309, 477
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 3 cut(s) 114, 675, 852
BcnI CCSGG 2 cut(s) 221, 871
BcoDI GTCTC 1 cut(s) 97
BcuI ACTAGT 1 cut(s) 808
BfaI CTAG 5 cut(s) 53, 356, 401, 800, 809
BfmI CTRYAG 1 cut(s) 179
BglII AGATCT 1 cut(s) 175
BisI GCNGC 2 cut(s) 364, 679
BlpI GCTNAGC 2 cut(s) 606, 876
BlsI GCNGC 2 cut(s) 365, 680
Bme1390I CCNGG 5 cut(s) 114, 221, 675, 852, 871
Bme18I GGWCC 3 cut(s) 284, 473, 836
BmgT120I GGNCC 6 cut(s) 36, 216, 217, 284, 473, 836
BmiI GGNNCC 5 cut(s) 218, 219, 285, 631, 837
BmrFI CCNGG 5 cut(s) 114, 221, 675, 852, 871
BmsI GCATC 1 cut(s) 427
BpiI GAAGAC 1 cut(s) 717
Bpu1102I GCTNAGC 2 cut(s) 606, 876
BpuEI CTTGAG 3 cut(s) 35, 148, 192
BpuMI CCSGG 2 cut(s) 221, 871
Bsa29I ATCGAT 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 556
BsaJI CCNNGG 2 cut(s) 219, 870
BsaWI WCCGGW 3 cut(s) 304, 475, 581
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 1 cut(s) 384
BseAI TCCGGA 2 cut(s) 304, 475
BseBI CCWGG 3 cut(s) 114, 675, 852
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 2 cut(s) 219, 870
BseGI GGATG 3 cut(s) 25, 442, 748
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 1 cut(s) 620
BseNI ACTGG 1 cut(s) 384
BseRI GAGGAG 2 cut(s) 494, 647
BseSI GKGCMC 2 cut(s) 220, 460
BseXI GCAGC 2 cut(s) 375, 665
BseYI CCCAGC 1 cut(s) 712
BsgI GTGCAG 3 cut(s) 525, 664, 714
Bsh1236I CGCG 3 cut(s) 135, 137, 912
BshFI GGCC 3 cut(s) 38, 218, 324
BshNI GGYRCC 1 cut(s) 629
BshVI ATCGAT 1 cut(s) 6
BsiHKAI GWGCWC 2 cut(s) 460, 555
BsiSI CCGG 5 cut(s) 221, 305, 476, 582, 870
BslFI GGGAC 3 cut(s) 297, 391, 849
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 1 cut(s) 97
BsmFI GGGAC 3 cut(s) 297, 391, 849
BsnI GGCC 3 cut(s) 38, 218, 324
Bsp120I GGGCCC 1 cut(s) 216
Bsp1286I GDGCHC 4 cut(s) 220, 460, 555, 607
Bsp13I TCCGGA 2 cut(s) 304, 475
Bsp1407I TGTACA 2 cut(s) 46, 257
Bsp143I GATC 5 cut(s) 3, 7, 175, 307, 703
Bsp1720I GCTNAGC 2 cut(s) 606, 876
BspACI CCGC 1 cut(s) 839
BspANI GGCC 3 cut(s) 38, 218, 324
BspCNI CTCAG 1 cut(s) 619
BspDI ATCGAT 1 cut(s) 6
BspEI TCCGGA 2 cut(s) 304, 475
BspFNI CGCG 3 cut(s) 135, 137, 912
BspLI GGNNCC 5 cut(s) 218, 219, 285, 631, 837
BspMAI CTGCAG 1 cut(s) 183
BspPI GGATC 3 cut(s) 2, 315, 711
BspT107I GGYRCC 1 cut(s) 629
BsrGI TGTACA 2 cut(s) 46, 257
BsrI ACTGG 1 cut(s) 384
BssECI CCNNGG 2 cut(s) 219, 870
BssMI GATC 5 cut(s) 3, 7, 175, 307, 703
Bst2UI CCWGG 3 cut(s) 114, 675, 852
Bst4CI ACNGT 2 cut(s) 46, 319
Bst6I CTCTTC 1 cut(s) 665
BstAPI GCANNNNNTGC 1 cut(s) 455
BstAUI TGTACA 2 cut(s) 46, 257
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 718
BstDEI CTNAG 3 cut(s) 344, 606, 876
BstEII GGTNACC 1 cut(s) 572
BstENI CCTNNNNNAGG 1 cut(s) 336
BstF5I GGATG 3 cut(s) 25, 442, 748
BstFNI CGCG 3 cut(s) 135, 137, 912
BstHHI GCGC 3 cut(s) 137, 442, 912
BstKTI GATC 5 cut(s) 6, 10, 178, 310, 706
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 5 cut(s) 3, 7, 175, 307, 703
BstMWI GCNNNNNNNGC 3 cut(s) 446, 455, 717
BstNI CCWGG 3 cut(s) 114, 675, 852
BstPI GGTNACC 1 cut(s) 572
BstSCI CCNGG 5 cut(s) 112, 219, 673, 850, 869
BstSFI CTRYAG 1 cut(s) 179
BstSLI GKGCMC 2 cut(s) 220, 460
BstUI CGCG 3 cut(s) 135, 137, 912
BstV1I GCAGC 2 cut(s) 375, 665
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 1 cut(s) 175
BstXI CCANNNNNNTGG 2 cut(s) 513, 858
BstYI RGATCY 1 cut(s) 175
Bsu15I ATCGAT 1 cut(s) 6
BsuRI GGCC 3 cut(s) 38, 218, 324
BsuTUI ATCGAT 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 741
BtsCI GGATG 3 cut(s) 25, 442, 748
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 1 cut(s) 537
Cac8I GCNNGC 1 cut(s) 718
CfoI GCGC 3 cut(s) 137, 442, 912
Cfr13I GGNCC 6 cut(s) 36, 216, 217, 284, 473, 836
ClaI ATCGAT 1 cut(s) 6
CsiI ACCWGGT 1 cut(s) 850
Csp6I GTAC 4 cut(s) 47, 157, 258, 671
CspCI CAANNNNNGTGG 2 cut(s) 792, 827
CviAII CATG 3 cut(s) 73, 445, 530
CviQI GTAC 4 cut(s) 47, 157, 258, 671
DdeI CTNAG 3 cut(s) 344, 606, 876
DpnI GATC 5 cut(s) 5, 9, 177, 309, 705
DpnII GATC 5 cut(s) 3, 7, 175, 307, 703
DrdI GACNNNNNNGTC 1 cut(s) 826
DseDI GACNNNNNNGTC 1 cut(s) 826
Eam1104I CTCTTC 1 cut(s) 665
EarI CTCTTC 1 cut(s) 665
Eco24I GRGCYC 2 cut(s) 220, 607
Eco47I GGWCC 3 cut(s) 284, 473, 836
Eco57I CTGAAG 1 cut(s) 251
Eco72I CACGTG 1 cut(s) 556
Eco91I GGTNACC 1 cut(s) 572
EcoNI CCTNNNNNAGG 1 cut(s) 336
EcoO109I RGGNCCY 1 cut(s) 217
EcoO65I GGTNACC 1 cut(s) 572
EcoRII CCWGG 3 cut(s) 112, 673, 850
EcoT38I GRGCYC 2 cut(s) 220, 607
FaeI CATG 3 cut(s) 76, 448, 533
FaiI YATR 8 cut(s) 74, 148, 228, 386, 446, 531, 848, 905
FaqI GGGAC 3 cut(s) 297, 391, 849
FatI CATG 3 cut(s) 72, 444, 529
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 2 cut(s) 364, 679
FokI GGATG 3 cut(s) 12, 449, 755
FriOI GRGCYC 2 cut(s) 220, 607
Fsp4HI GCNGC 2 cut(s) 364, 679
FspAI RTGCGCAY 1 cut(s) 441
FspBI CTAG 5 cut(s) 53, 356, 401, 800, 809
FspI TGCGCA 1 cut(s) 441
GlaI GCGC 3 cut(s) 136, 441, 911
GluI GCNGC 2 cut(s) 364, 679
GsaI CCCAGC 1 cut(s) 716
HaeIII GGCC 3 cut(s) 38, 218, 324
HapII CCGG 5 cut(s) 221, 305, 476, 582, 870
HhaI GCGC 3 cut(s) 137, 442, 912
Hin1II CATG 3 cut(s) 76, 448, 533
Hin6I GCGC 3 cut(s) 135, 440, 910
HinP1I GCGC 3 cut(s) 135, 440, 910
HincII GTYRAC 1 cut(s) 889
HindII GTYRAC 1 cut(s) 889
HindIII AAGCTT 2 cut(s) 290, 878
HinfI GANTC 3 cut(s) 22, 130, 885
HpaII CCGG 5 cut(s) 221, 305, 476, 582, 870
HphI GGTGA 2 cut(s) 584, 859
Hpy166II GTNNAC 5 cut(s) 258, 458, 589, 814, 889
Hpy188I TCNGA 1 cut(s) 781
Hpy188III TCNNGA 4 cut(s) 14, 305, 356, 476
Hpy8I GTNNAC 5 cut(s) 258, 458, 589, 814, 889
HpyAV CCTTC 3 cut(s) 98, 353, 419
HpyCH4III ACNGT 2 cut(s) 46, 319
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 7 cut(s) 181, 394, 458, 542, 646, 681, 731
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 455, 717
HpyF3I CTNAG 3 cut(s) 344, 606, 876
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 3 cut(s) 76, 448, 533
HspAI GCGC 3 cut(s) 135, 440, 910
Kpn2I TCCGGA 2 cut(s) 304, 475
Kzo9I GATC 5 cut(s) 3, 7, 175, 307, 703
LmnI GCTCC 2 cut(s) 223, 481
Lsp1109I GCAGC 2 cut(s) 375, 665
LweI GCATC 1 cut(s) 427
MabI ACCWGGT 1 cut(s) 850
MaeI CTAG 5 cut(s) 53, 356, 401, 800, 809
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 5 cut(s) 313, 334, 572, 595, 865
MalI GATC 5 cut(s) 5, 9, 177, 309, 705
MboI GATC 5 cut(s) 3, 7, 175, 307, 703
MboII GAAGA 7 cut(s) 185, 227, 413, 652, 717, 741, 744
MfeI CAATTG 1 cut(s) 168
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 4 cut(s) 220, 460, 555, 607
MluCI AATT 3 cut(s) 168, 201, 417
MlyI GAGTC 3 cut(s) 31, 139, 894
MnlI CCTC 7 cut(s) 21, 472, 553, 587, 659, 668, 729
MroI TCCGGA 2 cut(s) 304, 475
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 2 cut(s) 435, 463
MspI CCGG 5 cut(s) 221, 305, 476, 582, 870
MspR9I CCNGG 5 cut(s) 114, 221, 675, 852, 871
MunI CAATTG 1 cut(s) 168
MvaI CCWGG 3 cut(s) 114, 675, 852
MvnI CGCG 3 cut(s) 135, 137, 912
MwoI GCNNNNNNNGC 3 cut(s) 446, 455, 717
NciI CCSGG 2 cut(s) 221, 871
NdeII GATC 5 cut(s) 3, 7, 175, 307, 703
NlaIII CATG 3 cut(s) 76, 448, 533
NlaIV GGNNCC 5 cut(s) 218, 219, 285, 631, 837
NmuCI GTSAC 4 cut(s) 334, 572, 595, 865
NsbI TGCGCA 1 cut(s) 441
OliI CACNNNNGTG 1 cut(s) 463
PdmI GAANNNNTTC 1 cut(s) 521
PkrI GCNGC 2 cut(s) 365, 680
PleI GAGTC 3 cut(s) 30, 138, 893
PmaCI CACGTG 1 cut(s) 556
PmlI CACGTG 1 cut(s) 556
PpsI GAGTC 3 cut(s) 30, 138, 893
Ppu21I YACGTR 1 cut(s) 556
Psp6I CCWGG 3 cut(s) 112, 673, 850
PspCI CACGTG 1 cut(s) 556
PspEI GGTNACC 1 cut(s) 572
PspFI CCCAGC 1 cut(s) 712
PspGI CCWGG 3 cut(s) 112, 673, 850
PspN4I GGNNCC 5 cut(s) 218, 219, 285, 631, 837
PspOMI GGGCCC 1 cut(s) 216
PspPI GGNCC 6 cut(s) 36, 216, 217, 284, 473, 836
PstI CTGCAG 1 cut(s) 183
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 4 cut(s) 48, 158, 259, 672
RsaNI GTAC 4 cut(s) 47, 157, 258, 671
RseI CAYNNNNRTG 2 cut(s) 435, 463
SalI GTCGAC 1 cut(s) 887
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 2 cut(s) 364, 679
Sau3AI GATC 5 cut(s) 3, 7, 175, 307, 703
Sau96I GGNCC 6 cut(s) 36, 216, 217, 284, 473, 836
SchI GAGTC 3 cut(s) 31, 139, 894
ScrFI CCNGG 5 cut(s) 114, 221, 675, 852, 871
SduI GDGCHC 4 cut(s) 220, 460, 555, 607
SexAI ACCWGGT 1 cut(s) 850
SfaNI GCATC 1 cut(s) 427
SfcI CTRYAG 1 cut(s) 179
SinI GGWCC 3 cut(s) 284, 473, 836
SmiMI CAYNNNNRTG 2 cut(s) 435, 463
SmlI CTYRAG 3 cut(s) 14, 127, 207
SmoI CTYRAG 3 cut(s) 14, 127, 207
SpeI ACTAGT 1 cut(s) 808
Sse9I AATT 3 cut(s) 168, 201, 417
SsiI CCGC 1 cut(s) 839
SspI AATATT 1 cut(s) 920
SspMI CTAG 5 cut(s) 53, 356, 401, 800, 809
StyD4I CCNGG 5 cut(s) 112, 219, 673, 850, 869
TaaI ACNGT 2 cut(s) 46, 319
TaiI ACGT 1 cut(s) 558
TaqI TCGA 2 cut(s) 6, 888
TaqII GACCGA 1 cut(s) 301
TasI AATT 3 cut(s) 168, 201, 417
TatI WGTACW 3 cut(s) 46, 156, 257
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 1 cut(s) 544
TseFI GTSAC 4 cut(s) 334, 572, 595, 865
TseI GCWGC 2 cut(s) 363, 678
Tsp45I GTSAC 4 cut(s) 334, 572, 595, 865
TspDTI ATGAA 1 cut(s) 744
TspGWI ACGGA 1 cut(s) 755
TspRI CASTG 1 cut(s) 544
VneI GTGCAC 1 cut(s) 456
VpaK11BI GGWCC 3 cut(s) 284, 473, 836
XagI CCTNNNNNAGG 1 cut(s) 336
XapI RAATTY 1 cut(s) 417
XbaI TCTAGA 1 cut(s) 355
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 5 cut(s) 53, 356, 401, 800, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.