pycom10g23420

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004186
Physical Location & Seq
Reverse (-)
25576090 .. 25579256
3167 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1332 bp
ATGGCGTCCGCTTCTCTGATTCACCCGCGCTTTGGGCACTTACCGAGTTTCAGCTGCAATCGGCGAAACGACTCCCAAGGACCTTTAGGGACCTCCTCCTCCTGTTCGTCGTTCGGCTTCGCGTCCTATTCGATTTTTAGGACGATATATTATATTGCAGGCATATGTGGTAGTTGGAGAAACCAAGGCCTTAGGGCTCAAGCTATGACTAGTCAGGGAAATTTTGGATCCTCCAGGGGTGTTGCAAATGCGGAGAATAAACCCGATCATCTTCTCGTCCTTGTTCACGGCATCTTGGCGAGCCCGAGTGACTGGACATACGTGGAAGCGGAGTTAAAAAGGCGTCTAGGAAGAAACTTCTTAATCTATGCAAGTTCGTGTAACACGTACACCAAAACTTTTTCTGGTATTGATGGAGCTGGGAAACGATTAGCAGATGAAGTTTTGCAACTTGTACAAAAGACAGAGAGCCTAAAGAAGATCTCATTTTTAGCCCATTCTCTGGGGGGTTTATTTGCAAGATATGCAATTTCTGTCCTTTACACACCCAACGATAGTGCCAGTTCAACTACTGCAAATTCACAAACGGCATACTCCTTGAGACAAGGAAAGATTGCTGGGTTAGAGCCTATTAATTTTGTAACATTGGCAACTCCACATCTTGGGGTGCGAGGGAAAAAGCAGCTTCCATTTTTATTAGGAATACCTTTCTTAGAAAAGCTGGCTGCGCCAATCGCTCCTATTGTTGTTGGTCGAACTGGTAGTCAATTGTTTCTCACCGATGGTAAGCCTGAAAGACCACCACTTCTTCTGAGAATGGCATCCGATTGCAAAGATGGGAAATTTTTATCTGCTATGGGGGCATTCAAATCTCGGATTATCTATGCAAATGTATCTTATGATCATATGGTTGGTTGGCGCACATCATCCATTAGGAGGGAGACCGAAATTTTTAAGCCCCCTCGCCGGTCTTTGGACGGGTACAAGCATGTTGTAGACGTGGAGTACTGTCCTCCAGTTTCATCTGAAGGTCCCCATTTTCCTCCTGAAGCAGCTAAAGCAAAGGAAGCCGCCCAAAGCGAACCCAACATACAAAAGACGTTGGAATATCATGAAATTGTGGAAGATGAAATGATACGGGGTTTGCAGCAAGTAGGATGGAGAAAAGTTGATGTCAGCTTTCATTCGGCGTTCTGGCCCTTCTTTGCCCACAACAACATTCATGTGAAAAGTGAATGGCTTCACAATGCTGGCGTCGGGGTGGTTGCTCACGTTGCCGACAGCCTGAAGCAACAGGAATCCTCCTCCTCATTCATCACTGCCAGCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

444

Amino Acids

48.67

Weight (kDa)

9.35

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 86 - 308 2.1e-66 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 502, 662
AccI GTMKAC 1 cut(s) 996
AccII CGCG 2 cut(s) 28, 122
AciI CCGC 5 cut(s) 9, 26, 251, 329, 1071
AclWI GGATC 2 cut(s) 222, 235
AcsI RAATTY 4 cut(s) 220, 577, 842, 948
AcuI CTGAAG 3 cut(s) 1047, 1068, 1307
AcyI GRCGYC 3 cut(s) 5, 343, 1254
AfaI GTAC 4 cut(s) 389, 456, 983, 1007
AfiI CCNNNNNNNGG 6 cut(s) 32, 502, 662, 936, 966, 973
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 2 cut(s) 567, 868
AhlI ACTAGT 1 cut(s) 209
AjiI CACGTC 1 cut(s) 1000
AjnI CCWGG 1 cut(s) 233
AluBI AGCT 8 cut(s) 54, 203, 419, 685, 721, 1055, 1179, 1326
AluI AGCT 8 cut(s) 54, 203, 419, 685, 721, 1055, 1179, 1326
Alw26I GTCTC 2 cut(s) 595, 935
AlwI GGATC 2 cut(s) 222, 235
Ama87I CYCGRG 1 cut(s) 304
AoxI GGCC 2 cut(s) 187, 1196
ApeKI GCWGC 5 cut(s) 54, 682, 725, 1052, 1147
ApoI RAATTY 4 cut(s) 220, 577, 842, 948
ArsI GACNNNNNNTTYG 2 cut(s) 748, 780
AseI ATTAAT 1 cut(s) 633
Asp700I GAANNNNTTC 1 cut(s) 1239
AspLEI GCGC 3 cut(s) 30, 730, 921
AspS9I GGNCC 4 cut(s) 80, 90, 1031, 1197
AsuHPI GGTGA 2 cut(s) 14, 769
AvaI CYCGRG 1 cut(s) 304
AvaII GGWCC 3 cut(s) 80, 90, 1031
AxyI CCTNAGG 1 cut(s) 191
BaeGI GKGCMC 1 cut(s) 39
BamHI GGATCC 1 cut(s) 227
BanII GRGCYC 2 cut(s) 199, 305
BbvI GCAGC 5 cut(s) 41, 694, 712, 1064, 1159
BccI CCATC 4 cut(s) 407, 776, 830, 1152
BceAI ACGGC 2 cut(s) 304, 603
BciT130I CCWGG 1 cut(s) 235
BclI TGATCA 1 cut(s) 901
BcoDI GTCTC 2 cut(s) 595, 935
BcuI ACTAGT 1 cut(s) 209
BfaI CTAG 2 cut(s) 210, 347
BglII AGATCT 1 cut(s) 480
BisI GCNGC 6 cut(s) 55, 683, 726, 1053, 1071, 1148
BlsI GCNGC 6 cut(s) 56, 684, 727, 1054, 1072, 1149
BmcAI AGTACT 1 cut(s) 1007
Bme1390I CCNGG 1 cut(s) 235
Bme18I GGWCC 3 cut(s) 80, 90, 1031
BmeT110I CYCGRG 1 cut(s) 304
BmgBI CACGTC 1 cut(s) 1000
BmgT120I GGNCC 4 cut(s) 80, 90, 1031, 1197
BmiI GGNNCC 3 cut(s) 91, 229, 1033
BmrFI CCNGG 1 cut(s) 235
BmsI GCATC 2 cut(s) 300, 830
BpmI CTGGAG 2 cut(s) 217, 999
BpuEI CTTGAG 2 cut(s) 183, 619
BsaAI YACGTR 2 cut(s) 322, 387
BsaHI GRCGYC 3 cut(s) 5, 343, 1254
BsaI GGTCTC 1 cut(s) 935
BsaJI CCNNGG 3 cut(s) 76, 184, 234
Bsc4I CCNNNNNNNGG 6 cut(s) 32, 502, 662, 936, 966, 973
Bse118I RCCGGY 1 cut(s) 966
Bse1I ACTGG 4 cut(s) 317, 561, 763, 1016
Bse21I CCTNAGG 1 cut(s) 191
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 3 cut(s) 76, 184, 234
BseGI GGATG 3 cut(s) 821, 926, 1163
BseLI CCNNNNNNNGG 6 cut(s) 32, 502, 662, 936, 966, 973
BseMII CTCAG 1 cut(s) 803
BseNI ACTGG 4 cut(s) 317, 561, 763, 1016
BseRI GAGGAG 4 cut(s) 85, 88, 1294, 1297
BseSI GKGCMC 1 cut(s) 39
BseXI GCAGC 5 cut(s) 41, 694, 712, 1064, 1159
BseYI CCCAGC 2 cut(s) 419, 617
Bsh1236I CGCG 2 cut(s) 28, 122
BshFI GGCC 2 cut(s) 189, 1198
BsiHKCI CYCGRG 1 cut(s) 304
BsiSI CCGG 1 cut(s) 967
BslFI GGGAC 2 cut(s) 103, 1017
BslI CCNNNNNNNGG 6 cut(s) 32, 502, 662, 936, 966, 973
BsmAI GTCTC 2 cut(s) 595, 935
BsmFI GGGAC 2 cut(s) 103, 1017
BsmI GAATGC 1 cut(s) 863
BsnI GGCC 2 cut(s) 189, 1198
Bso31I GGTCTC 1 cut(s) 935
BsoBI CYCGRG 1 cut(s) 304
Bsp1286I GDGCHC 3 cut(s) 39, 199, 305
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 4 cut(s) 227, 265, 480, 901
BspACI CCGC 5 cut(s) 9, 26, 251, 329, 1071
BspANI GGCC 2 cut(s) 189, 1198
BspCNI CTCAG 1 cut(s) 804
BspFNI CGCG 2 cut(s) 28, 122
BspHI TCATGA 1 cut(s) 1111
BspLI GGNNCC 3 cut(s) 91, 229, 1033
BspPI GGATC 2 cut(s) 222, 235
BspTNI GGTCTC 1 cut(s) 935
BsrFI RCCGGY 1 cut(s) 966
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 4 cut(s) 317, 561, 763, 1016
BssAI RCCGGY 1 cut(s) 966
BssECI CCNNGG 3 cut(s) 76, 184, 234
BssMI GATC 4 cut(s) 227, 265, 480, 901
BssNI GRCGYC 3 cut(s) 5, 343, 1254
BssT1I CCWWGG 2 cut(s) 76, 184
Bst2UI CCWGG 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 1010
BstACI GRCGYC 3 cut(s) 5, 343, 1254
BstAPI GCANNNNNTGC 1 cut(s) 524
BstAUI TGTACA 1 cut(s) 454
BstBAI YACGTR 2 cut(s) 322, 387
BstC8I GCNNGC 5 cut(s) 160, 301, 723, 1252, 1324
BstDEI CTNAG 3 cut(s) 191, 712, 812
BstF5I GGATG 3 cut(s) 821, 926, 1163
BstFNI CGCG 2 cut(s) 28, 122
BstHHI GCGC 3 cut(s) 30, 730, 921
BstKTI GATC 4 cut(s) 230, 268, 483, 904
BstMAI GTCTC 2 cut(s) 595, 935
BstMBI GATC 4 cut(s) 227, 265, 480, 901
BstMWI GCNNNNNNNGC 8 cut(s) 34, 524, 727, 734, 860, 1058, 1067, 1274
BstNI CCWGG 1 cut(s) 235
BstNSI RCATGY 1 cut(s) 992
BstSCI CCNGG 1 cut(s) 233
BstSLI GKGCMC 1 cut(s) 39
BstUI CGCG 2 cut(s) 28, 122
BstV1I GCAGC 5 cut(s) 41, 694, 712, 1064, 1159
BstX2I RGATCY 2 cut(s) 227, 480
BstYI RGATCY 2 cut(s) 227, 480
Bsu36I CCTNAGG 1 cut(s) 191
BsuRI GGCC 2 cut(s) 189, 1198
BtrI CACGTC 1 cut(s) 1000
BtsCI GGATG 3 cut(s) 821, 926, 1163
BtsI GCAGTG 1 cut(s) 1317
BtsIMutI CAGTG 1 cut(s) 1317
Cac8I GCNNGC 5 cut(s) 160, 301, 723, 1252, 1324
CciI TCATGA 1 cut(s) 1111
CfoI GCGC 3 cut(s) 30, 730, 921
Cfr10I RCCGGY 1 cut(s) 966
Cfr13I GGNCC 4 cut(s) 80, 90, 1031, 1197
CseI GACGC 3 cut(s) 111, 332, 1243
Csp6I GTAC 4 cut(s) 388, 455, 982, 1006
CviAII CATG 3 cut(s) 989, 1112, 1223
CviQI GTAC 4 cut(s) 388, 455, 982, 1006
DdeI CTNAG 3 cut(s) 191, 712, 812
DpnI GATC 4 cut(s) 229, 267, 482, 903
DpnII GATC 4 cut(s) 227, 265, 480, 901
Eco130I CCWWGG 2 cut(s) 76, 184
Eco147I AGGCCT 1 cut(s) 189
Eco24I GRGCYC 2 cut(s) 199, 305
Eco31I GGTCTC 1 cut(s) 935
Eco47I GGWCC 3 cut(s) 80, 90, 1031
Eco57I CTGAAG 3 cut(s) 1047, 1068, 1307
Eco81I CCTNAGG 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 304
EcoO109I RGGNCCY 3 cut(s) 80, 90, 1031
EcoRII CCWGG 1 cut(s) 233
EcoT14I CCWWGG 2 cut(s) 76, 184
EcoT38I GRGCYC 2 cut(s) 199, 305
ErhI CCWWGG 2 cut(s) 76, 184
FaeI CATG 3 cut(s) 992, 1115, 1226
FaqI GGGAC 2 cut(s) 103, 1017
FatI CATG 3 cut(s) 988, 1111, 1222
FauI CCCGC 1 cut(s) 33
FauNDI CATATG 2 cut(s) 164, 906
FbaI TGATCA 1 cut(s) 901
FblI GTMKAC 1 cut(s) 996
Fnu4HI GCNGC 6 cut(s) 55, 683, 726, 1053, 1071, 1148
FokI GGATG 3 cut(s) 808, 913, 1170
FriOI GRGCYC 2 cut(s) 199, 305
Fsp4HI GCNGC 6 cut(s) 55, 683, 726, 1053, 1071, 1148
FspBI CTAG 2 cut(s) 210, 347
GlaI GCGC 3 cut(s) 29, 729, 920
GluI GCNGC 6 cut(s) 55, 683, 726, 1053, 1071, 1148
GsaI CCCAGC 2 cut(s) 423, 621
GsuI CTGGAG 2 cut(s) 217, 999
HaeIII GGCC 2 cut(s) 189, 1198
HapII CCGG 1 cut(s) 967
HgaI GACGC 3 cut(s) 111, 332, 1243
HhaI GCGC 3 cut(s) 30, 730, 921
Hin1I GRCGYC 3 cut(s) 5, 343, 1254
Hin1II CATG 3 cut(s) 992, 1115, 1226
Hin6I GCGC 3 cut(s) 28, 728, 919
HinP1I GCGC 3 cut(s) 28, 728, 919
HinfI GANTC 3 cut(s) 19, 71, 1298
HpaII CCGG 1 cut(s) 967
HphI GGTGA 2 cut(s) 14, 769
Hpy166II GTNNAC 3 cut(s) 286, 390, 997
Hpy188I TCNGA 5 cut(s) 18, 813, 826, 876, 1027
Hpy188III TCNNGA 2 cut(s) 1046, 1112
Hpy8I GTNNAC 3 cut(s) 286, 390, 997
Hpy99I CGWCG 2 cut(s) 112, 1259
HpyAV CCTTC 2 cut(s) 1022, 1210
HpyCH4III ACNGT 1 cut(s) 1010
HpyCH4IV ACGT 5 cut(s) 321, 386, 999, 1100, 1272
HpyF10VI GCNNNNNNNGC 8 cut(s) 34, 524, 727, 734, 860, 1058, 1067, 1274
HpyF3I CTNAG 3 cut(s) 191, 712, 812
HpySE526I ACGT 5 cut(s) 321, 386, 999, 1100, 1272
Hsp92I GRCGYC 3 cut(s) 5, 343, 1254
Hsp92II CATG 3 cut(s) 992, 1115, 1226
HspAI GCGC 3 cut(s) 28, 728, 919
Ksp22I TGATCA 1 cut(s) 901
Kzo9I GATC 4 cut(s) 227, 265, 480, 901
LmnI GCTCC 2 cut(s) 416, 742
Lsp1109I GCAGC 5 cut(s) 41, 694, 712, 1064, 1159
LweI GCATC 2 cut(s) 300, 830
MaeI CTAG 2 cut(s) 210, 347
MaeII ACGT 5 cut(s) 321, 386, 999, 1100, 1272
MaeIII GTNAC 3 cut(s) 308, 380, 640
MalI GATC 4 cut(s) 229, 267, 482, 903
MboI GATC 4 cut(s) 227, 265, 480, 901
MboII GAAGA 5 cut(s) 263, 363, 490, 800, 1136
MfeI CAATTG 1 cut(s) 767
MflI RGATCY 2 cut(s) 227, 480
MhlI GDGCHC 3 cut(s) 39, 199, 305
MluCI AATT 8 cut(s) 220, 528, 577, 634, 767, 842, 948, 1116
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 2 cut(s) 155, 1083
MroXI GAANNNNTTC 1 cut(s) 1239
MseI TTAA 4 cut(s) 335, 362, 633, 954
MslI CAYNNNNRTG 1 cut(s) 1223
MspA1I CMGCKG 1 cut(s) 54
MspI CCGG 1 cut(s) 967
MspR9I CCNGG 1 cut(s) 235
MunI CAATTG 1 cut(s) 767
Mva1269I GAATGC 1 cut(s) 863
MvaI CCWGG 1 cut(s) 235
MvnI CGCG 2 cut(s) 28, 122
MwoI GCNNNNNNNGC 8 cut(s) 34, 524, 727, 734, 860, 1058, 1067, 1274
NdeI CATATG 2 cut(s) 164, 906
NdeII GATC 4 cut(s) 227, 265, 480, 901
NlaIII CATG 3 cut(s) 992, 1115, 1226
NlaIV GGNNCC 3 cut(s) 91, 229, 1033
NmuCI GTSAC 1 cut(s) 308
NspI RCATGY 1 cut(s) 992
PagI TCATGA 1 cut(s) 1111
PceI AGGCCT 1 cut(s) 189
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PctI GAATGC 1 cut(s) 863
PdmI GAANNNNTTC 1 cut(s) 1239
PfeI GAWTC 2 cut(s) 19, 1298
PflMI CCANNNNNTGG 2 cut(s) 502, 662
PkrI GCNGC 6 cut(s) 56, 684, 727, 1054, 1072, 1149
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
Ppu21I YACGTR 2 cut(s) 322, 387
PpuMI RGGWCCY 3 cut(s) 80, 90, 1031
PshBI ATTAAT 1 cut(s) 633
Psp5II RGGWCCY 3 cut(s) 80, 90, 1031
Psp6I CCWGG 1 cut(s) 233
PspFI CCCAGC 2 cut(s) 419, 617
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 3 cut(s) 91, 229, 1033
PspPI GGNCC 4 cut(s) 80, 90, 1031, 1197
PspPPI RGGWCCY 3 cut(s) 80, 90, 1031
PsrI GAACNNNNNNTAC 2 cut(s) 1074, 1106
PsuI RGATCY 2 cut(s) 227, 480
PvuII CAGCTG 1 cut(s) 54
RsaI GTAC 4 cut(s) 389, 456, 983, 1007
RsaNI GTAC 4 cut(s) 388, 455, 982, 1006
RseI CAYNNNNRTG 1 cut(s) 1223
SaqAI TTAA 4 cut(s) 335, 362, 633, 954
SatI GCNGC 6 cut(s) 55, 683, 726, 1053, 1071, 1148
Sau3AI GATC 4 cut(s) 227, 265, 480, 901
Sau96I GGNCC 4 cut(s) 80, 90, 1031, 1197
ScaI AGTACT 1 cut(s) 1007
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 235
SduI GDGCHC 3 cut(s) 39, 199, 305
SfaNI GCATC 2 cut(s) 300, 830
SinI GGWCC 3 cut(s) 80, 90, 1031
SmiMI CAYNNNNRTG 1 cut(s) 1223
SmlI CTYRAG 2 cut(s) 198, 598
SmoI CTYRAG 2 cut(s) 198, 598
SpeI ACTAGT 1 cut(s) 209
Sse9I AATT 8 cut(s) 220, 528, 577, 634, 767, 842, 948, 1116
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 5 cut(s) 9, 26, 251, 329, 1071
SspMI CTAG 2 cut(s) 210, 347
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 233
StyI CCWWGG 2 cut(s) 76, 184
TaaI ACNGT 1 cut(s) 1010
TaiI ACGT 5 cut(s) 324, 389, 1002, 1103, 1275
TaqI TCGA 2 cut(s) 131, 754
TaqII GACCGA 1 cut(s) 959
TasI AATT 8 cut(s) 220, 528, 577, 634, 767, 842, 948, 1116
TatI WGTACW 2 cut(s) 454, 1005
TauI GCSGC 1 cut(s) 1073
TfiI GAWTC 2 cut(s) 19, 1298
Tru1I TTAA 4 cut(s) 335, 362, 633, 954
Tru9I TTAA 4 cut(s) 335, 362, 633, 954
TscAI CASTG 1 cut(s) 1324
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 5 cut(s) 54, 682, 725, 1052, 1147
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 7 cut(s) 453, 1011, 1128, 1143, 1172, 1211, 1303
TspRI CASTG 1 cut(s) 1324
Van91I CCANNNNNTGG 2 cut(s) 502, 662
VpaK11BI GGWCC 3 cut(s) 80, 90, 1031
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 4 cut(s) 220, 577, 842, 948
XceI RCATGY 1 cut(s) 992
XmiI GTMKAC 1 cut(s) 996
XmnI GAANNNNTTC 1 cut(s) 1239
XspI CTAG 2 cut(s) 210, 347
ZrmI AGTACT 1 cut(s) 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.