pycom10g24360

Auxin-repressed 12.5 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005809
Physical Location & Seq
Forward (+)
26240063 .. 26241393
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 462 bp
ATGTTAACGCTACCAGCTGGAAGTAGCAAAACAAAAGATGAAAACAAAAGATGGCAAATTAATAGAAAGAGAGAGAAAGAAAGAAAGAAAGAGAAGAGAGAGAGAGAGAGAGCAAAAATGGTTCTTCTTGAAAAGCTTTGGGATGATATTGTCGCCGGACCTCAGCCTGAACGCGGCCTCGGCAGGCTGAGGAAGGTGTCTCCTAAGCCCTTGAACGCAAAAGAAGGGGAAGGAGAGTCGAGCAAGTTGGCGATGCCGATGAGCCCGGCAACACCAGGAACCCCAGGCACACCAGTATCTGCTCGTGCTAAAGACAACGTTTGGAGGAGTGTCTTCCACCCAGGTAGCAACCTTGCCTCCAAATCCATGGGCAACCAGGTTTTCGACAAGCCACAGCCCAACTCTCCCACTGTCTACGACTGGCTCTACAGTGGGGAGACCAGGAGCAAGCATCATCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

154

Amino Acids

17.31

Weight (kDa)

10.25

Isoelectric Point (pI)

55.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_repressed PF05564 46 - 152 8.1e-48 Dormancy/auxin associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 414
AccII CGCG 1 cut(s) 174
AciI CCGC 1 cut(s) 174
AclI AACGTT 1 cut(s) 318
AfiI CCNNNNNNNGG 1 cut(s) 173
AgsI TTSAA 2 cut(s) 131, 214
AjnI CCWGG 5 cut(s) 274, 283, 340, 375, 440
AluBI AGCT 2 cut(s) 17, 136
AluI AGCT 2 cut(s) 17, 136
Alw26I GTCTC 2 cut(s) 204, 431
AlwNI CAGNNNCTG 1 cut(s) 299
AoxI GGCC 1 cut(s) 175
AseI ATTAAT 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 158
AsuC2I CCSGG 1 cut(s) 266
AvaII GGWCC 1 cut(s) 158
BaeI ACNNNNGTAYC 2 cut(s) 279, 312
BanII GRGCYC 1 cut(s) 266
BauI CACGAG 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 325
BbvCI CCTCAGC 2 cut(s) 162, 188
BccI CCATC 1 cut(s) 45
BciT130I CCWGG 5 cut(s) 276, 285, 342, 377, 442
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 2 cut(s) 204, 431
BfmI CTRYAG 1 cut(s) 427
BisI GCNGC 1 cut(s) 175
BlsI GCNGC 1 cut(s) 176
Bme1390I CCNGG 6 cut(s) 266, 276, 285, 342, 377, 442
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 6 cut(s) 266, 276, 285, 342, 377, 442
BmsI GCATC 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 325
Bpu10I CCTNAGC 3 cut(s) 162, 188, 204
BpuMI CCSGG 1 cut(s) 266
BsaI GGTCTC 1 cut(s) 431
BsaJI CCNNGG 4 cut(s) 178, 283, 340, 366
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse1I ACTGG 2 cut(s) 293, 425
BseBI CCWGG 5 cut(s) 276, 285, 342, 377, 442
BseDI CCNNGG 4 cut(s) 178, 283, 340, 366
BseGI GGATG 1 cut(s) 148
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMII CTCAG 2 cut(s) 176, 179
BseNI ACTGG 2 cut(s) 293, 425
BseRI GAGGAG 1 cut(s) 340
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 177
BsiSI CCGG 2 cut(s) 156, 266
BslI CCNNNNNNNGG 1 cut(s) 173
BsmAI GTCTC 2 cut(s) 204, 431
BsnI GGCC 1 cut(s) 177
Bso31I GGTCTC 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 266
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 1 cut(s) 174
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 2 cut(s) 175, 180
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 1 cut(s) 280
BspTNI GGTCTC 1 cut(s) 431
BsrI ACTGG 2 cut(s) 293, 425
BssECI CCNNGG 4 cut(s) 178, 283, 340, 366
BssSI CACGAG 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 366
Bst2BI CACGAG 1 cut(s) 303
Bst2UI CCWGG 5 cut(s) 276, 285, 342, 377, 442
Bst4CI ACNGT 2 cut(s) 412, 431
Bst6I CTCTTC 1 cut(s) 89
BstC8I GCNNGC 2 cut(s) 185, 449
BstDEI CTNAG 3 cut(s) 162, 188, 204
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 148
BstFNI CGCG 1 cut(s) 174
BstMAI GTCTC 2 cut(s) 204, 431
BstMWI GCNNNNNNNGC 1 cut(s) 180
BstNI CCWGG 5 cut(s) 276, 285, 342, 377, 442
BstSCI CCNGG 6 cut(s) 264, 274, 283, 340, 375, 440
BstSFI CTRYAG 1 cut(s) 427
BstUI CGCG 1 cut(s) 174
BstV2I GAAGAC 1 cut(s) 325
BstXI CCANNNNNNTGG 1 cut(s) 367
BsuRI GGCC 1 cut(s) 177
BtgI CCRYGG 1 cut(s) 366
BtgZI GCGATG 1 cut(s) 266
BtsCI GGATG 1 cut(s) 148
BtsIMutI CAGTG 2 cut(s) 408, 436
Cac8I GCNNGC 2 cut(s) 185, 449
CaiI CAGNNNCTG 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 158
CsiI ACCWGGT 1 cut(s) 375
CviAII CATG 1 cut(s) 367
DdeI CTNAG 3 cut(s) 162, 188, 204
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 266
Eco31I GGTCTC 1 cut(s) 431
Eco47I GGWCC 1 cut(s) 158
EcoRII CCWGG 5 cut(s) 274, 283, 340, 375, 440
EcoT14I CCWWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 266
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 1 cut(s) 370
FaiI YATR 1 cut(s) 368
FatI CATG 1 cut(s) 366
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 1 cut(s) 175
FokI GGATG 1 cut(s) 155
FriOI GRGCYC 1 cut(s) 266
Fsp4HI GCNGC 1 cut(s) 175
GluI GCNGC 1 cut(s) 175
HaeIII GGCC 1 cut(s) 177
HapII CCGG 2 cut(s) 156, 266
Hin1II CATG 1 cut(s) 370
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 1 cut(s) 236
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 2 cut(s) 156, 266
Hpy166II GTNNAC 2 cut(s) 6, 415
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 2 cut(s) 6, 415
HpyAV CCTTC 3 cut(s) 187, 218, 224
HpyCH4III ACNGT 2 cut(s) 412, 431
HpyCH4IV ACGT 1 cut(s) 318
HpyF10VI GCNNNNNNNGC 1 cut(s) 180
HpyF3I CTNAG 3 cut(s) 162, 188, 204
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 1 cut(s) 370
KspAI GTTAAC 1 cut(s) 6
LmnI GCTCC 1 cut(s) 444
LweI GCATC 1 cut(s) 243
MabI ACCWGGT 1 cut(s) 375
MaeII ACGT 1 cut(s) 318
MboII GAAGA 3 cut(s) 106, 116, 325
MhlI GDGCHC 1 cut(s) 266
MluCI AATT 1 cut(s) 57
MlyI GAGTC 1 cut(s) 245
MnlI CCTC 5 cut(s) 171, 183, 188, 318, 367
MseI TTAA 3 cut(s) 5, 60, 460
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 156, 266
MspR9I CCNGG 6 cut(s) 266, 276, 285, 342, 377, 442
MvaI CCWGG 5 cut(s) 276, 285, 342, 377, 442
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 180
NciI CCSGG 1 cut(s) 266
NcoI CCATGG 1 cut(s) 366
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 1 cut(s) 280
NmeAIII GCCGAG 1 cut(s) 159
PkrI GCNGC 1 cut(s) 176
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
PshBI ATTAAT 1 cut(s) 60
Psp1406I AACGTT 1 cut(s) 318
Psp6I CCWGG 5 cut(s) 274, 283, 340, 375, 440
PspGI CCWGG 5 cut(s) 274, 283, 340, 375, 440
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 17
SaqAI TTAA 3 cut(s) 5, 60, 460
SatI GCNGC 1 cut(s) 175
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 6 cut(s) 266, 276, 285, 342, 377, 442
SduI GDGCHC 1 cut(s) 266
SetI ASST 8 cut(s) 19, 138, 163, 198, 321, 346, 354, 381
SexAI ACCWGGT 1 cut(s) 375
SfaNI GCATC 1 cut(s) 243
SfcI CTRYAG 1 cut(s) 427
SinI GGWCC 1 cut(s) 158
Sse9I AATT 1 cut(s) 57
SsiI CCGC 1 cut(s) 174
StyD4I CCNGG 6 cut(s) 264, 274, 283, 340, 375, 440
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 2 cut(s) 412, 431
TaiI ACGT 1 cut(s) 321
TaqI TCGA 2 cut(s) 239, 384
TasI AATT 1 cut(s) 57
TauI GCSGC 1 cut(s) 177
Tru1I TTAA 3 cut(s) 5, 60, 460
Tru9I TTAA 3 cut(s) 5, 60, 460
TscAI CASTG 2 cut(s) 415, 436
TspDTI ATGAA 1 cut(s) 54
TspRI CASTG 2 cut(s) 415, 436
VpaK11BI GGWCC 1 cut(s) 158
VspI ATTAAT 1 cut(s) 60
XmiI GTMKAC 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.