pycom10g25000

Wings apart-like protein regulation of heterochromatin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009354
Physical Location & Seq
Forward (+)
26712724 .. 26717318
4595 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1815 bp
ATGGAAAAGCCGGAGTTATGCCCAAAATGGATAGCTTTGCTGACTATTGAGAAAGCTTGCTTATCTACTATTTCTCTTGAAGAAACCACTGGCGCAGTAAGGAAGTCAGGGGGCAACTTTAAGGAAAAATTACGAGAGCTTGGAGGTCTAGATGCTGTATTTGAGGTGACCATGGGTTGCCATTCCAATTTGGAGGGTTGGCTAAAAGATAGCTCGCATTCTATTTGGGAAAAGGAAATCGACAAGGTGCGGAGCCTGGTTCTTCTTTTGAAATGTTTGAAGATCATGGAAAATGCGACATTCCTAAGTAAAGAGAATCAGAGCCATTTGCTAGGAATGAAAAGGAAGTTGGATCCCATGGCAATTCCTATGTCTTTTACAGAACTTGTTATAAGTGCCATCAATATTCTATCAGGTCTTTATCTGCAGGAAAGTTCCTCATCTGCCTCTAATGAAGAAAAATCTTATAGTCTCTCTAATGGGGTTGAACATGTTTCTGAGAAGAGATCCGATATATGTCAGAGCAATCAGTTAATGTCAACGCCTCGCTCAGGGTATACTATATTCAGTTCGGAAACTACCAGCACCTCCATGACTGATGCTTATTCATTGAAGACAAGACTCAATTCTTCTAGAAATGGTTCTTCCAATGGTGTATCAAGCCATGTAAGCAGTGGAATCGGTAAATTTAGTAATCTTTCCACGAGGAATGCTGGTTTTAGACGGCGATCCTCTAACTTTGACGACTCCAAAATTGATCTTTCAGAAGAGAGTCAAGATCCTTTCGCATTTAGCGATGATTCCAGGATGGATGCTGATCTCAGTCAGAGATCCTATATTTCCCAAGACTCGAAGACTGATCTTTCACAGGGGAGTCAAGATCCTTTTGCATTTGATGAAGATGATTATAAACCTTCTAAGTGGGACAAGTTATCTGGCAAGAAAAAGTTCTCTCTTTCGCAAGAGAATGCAACAGCATACAGAGAAATTGATGACATTTGTCAATTGCAGCTTATTATGAGCCAAGAAGCATCGAGTAATGGAGAAAACCATCAGACGCAAGAGACCACCTGTTCAGGTGCTGTTAACAGAGAAGGTTCCAGTCTTGTAGCAGACTGCCTTCTTACCGCTGTCAAGGTTCTGATGAACTTAGCAAATGATAATCCTGTTGGGTGTCAGCAAATTGCTGCCTATGGAGGACTGGAAACCTTGTCTTCCTTGATTGCCAACCACTTTCCATCTTTCAACTCATTATCTTCTCCCTTCAGCGAGAGAAGTGAGAATACTTCCAGCATGGAACTTGACCATCAGAATGATAAACGTCTCACTGATCAAGAGCTGGACTTTCTTGTTGCTATTTTAGGCCTGCTTGTGAACTTAGTGGAGAAAGATGGCCAAAACAGATCGCGTCTTGCAGCAGCTAGTGTTCACTTACCTAGTTCAGAAGGGTTTAAAGAGGGGAGTCGCAAGGATCTAATTCTGCTAATCTGCTCTATCTTTCTAGCCAACCAAGGTGCAGGCGAGGGAGGTGGAGAAGAAACGATTTTACCAACTGATGAGGCAGCTGTCTTACAAGGAGAACAGGAAGCGGAGAAAATGATAGTAGAAGCTTATTCTGCCTTGCTTCTAGCATTTCTTTCAACTGAGAGTAAGAGTATACGTGATGCAATTTCTGACTGTCTCCCAGACCGCAACCTGGCTATCCTAGTACCTGTGTTGGACAGATTTGTGGCATTCCATTTGACGCTAAACATGATATCGCCCGAGACTCATAAAGCTGTGAGCGAAGTAATTGAATCATGTAGGATACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WAPL

Protein Analysis

605

Amino Acids

66.29

Weight (kDa)

4.98

Isoelectric Point (pI)

52.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAPL PF07814 5 - 465 8.4e-18 Wings apart-like protein regulation of heterochromatin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 392, 909
AccI GTMKAC 2 cut(s) 557, 1657
AccII CGCG 1 cut(s) 1408
AciI CCGC 4 cut(s) 250, 1128, 1589, 1690
AclWI GGATC 8 cut(s) 347, 360, 501, 723, 773, 825, 875, 1479
AcoI YGGCCR 1 cut(s) 1393
AcsI RAATTY 1 cut(s) 686
AcuI CTGAAG 1 cut(s) 1249
AfaI GTAC 1 cut(s) 1710
AfiI CCNNNNNNNGG 2 cut(s) 551, 1696
AflIII ACRYGT 1 cut(s) 490
AgsI TTSAA 8 cut(s) 80, 271, 280, 488, 613, 1246, 1641, 1796
AjnI CCWGG 3 cut(s) 255, 803, 1695
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
Alw26I GTCTC 5 cut(s) 476, 1058, 1328, 1685, 1760
AlwI GGATC 8 cut(s) 347, 360, 501, 723, 773, 825, 875, 1479
AlwNI CAGNNNCTG 1 cut(s) 1082
Ama87I CYCGRG 1 cut(s) 1763
AoxI GGCC 2 cut(s) 1363, 1393
ApeKI GCWGC 5 cut(s) 1009, 1187, 1415, 1418, 1562
ApoI RAATTY 1 cut(s) 686
Asp700I GAANNNNTTC 2 cut(s) 640, 947
AspLEI GCGC 1 cut(s) 95
AsuHPI GGTGA 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 1763
BalI TGGCCA 1 cut(s) 1395
BamHI GGATCC 1 cut(s) 352
BarI GAAGNNNNNNTAC 2 cut(s) 1267, 1299
BauI CACGAG 1 cut(s) 703
BbsI GAAGAC 3 cut(s) 620, 860, 1206
BbvI GCAGC 5 cut(s) 1021, 1174, 1427, 1430, 1574
BccI CCATC 6 cut(s) 407, 802, 1059, 1246, 1314, 1385
BceAI ACGGC 1 cut(s) 740
BcgI CGANNNNNNTGC 2 cut(s) 661, 695
BciT130I CCWGG 3 cut(s) 257, 805, 1697
BciVI GTATCC 1 cut(s) 1800
BclI TGATCA 1 cut(s) 1330
BcoDI GTCTC 5 cut(s) 476, 1058, 1328, 1685, 1760
BfaI CTAG 8 cut(s) 149, 332, 633, 1422, 1437, 1502, 1628, 1706
BfmI CTRYAG 1 cut(s) 425
BfuI GTATCC 1 cut(s) 1800
BisI GCNGC 5 cut(s) 1010, 1188, 1416, 1419, 1563
BlsI GCNGC 5 cut(s) 1011, 1189, 1417, 1420, 1564
Bme1390I CCNGG 3 cut(s) 257, 805, 1697
BmeT110I CYCGRG 1 cut(s) 1763
BmiI GGNNCC 3 cut(s) 254, 354, 1099
BmrFI CCNGG 3 cut(s) 257, 805, 1697
BmsI GCATC 5 cut(s) 142, 589, 802, 1040, 1654
BoxI GACNNNNGTC 1 cut(s) 999
BpiI GAAGAC 3 cut(s) 620, 860, 1206
Bpu10I CCTNAGC 1 cut(s) 550
BsaAI YACGTR 1 cut(s) 1661
BsaBI GATNNNNATC 1 cut(s) 816
BsaI GGTCTC 1 cut(s) 1058
BsaJI CCNNGG 3 cut(s) 171, 357, 1510
Bsc4I CCNNNNNNNGG 2 cut(s) 551, 1696
Bse1I ACTGG 3 cut(s) 94, 1101, 1206
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 3 cut(s) 257, 805, 1697
BseDI CCNNGG 3 cut(s) 171, 357, 1510
BseGI GGATG 2 cut(s) 813, 817
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 2 cut(s) 551, 1696
BseMII CTCAG 4 cut(s) 489, 564, 835, 1635
BseNI ACTGG 3 cut(s) 94, 1101, 1206
BseXI GCAGC 5 cut(s) 1021, 1174, 1427, 1430, 1574
BsgI GTGCAG 1 cut(s) 1536
Bsh1236I CGCG 1 cut(s) 1408
BshFI GGCC 2 cut(s) 1365, 1395
BsiHKCI CYCGRG 1 cut(s) 1763
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 938
BslI CCNNNNNNNGG 2 cut(s) 551, 1696
BsmAI GTCTC 5 cut(s) 476, 1058, 1328, 1685, 1760
BsmBI CGTCTC 1 cut(s) 1328
BsmFI GGGAC 1 cut(s) 938
BsmI GAATGC 4 cut(s) 217, 715, 973, 1733
BsnI GGCC 2 cut(s) 1365, 1395
Bso31I GGTCTC 1 cut(s) 1058
BsoBI CYCGRG 1 cut(s) 1763
Bsp19I CCATGG 2 cut(s) 171, 357
BspACI CCGC 4 cut(s) 250, 1128, 1589, 1690
BspANI GGCC 2 cut(s) 1365, 1395
BspCNI CTCAG 4 cut(s) 490, 563, 834, 1636
BspFNI CGCG 1 cut(s) 1408
BspLI GGNNCC 3 cut(s) 254, 354, 1099
BspMAI CTGCAG 1 cut(s) 429
BspPI GGATC 8 cut(s) 347, 360, 501, 723, 773, 825, 875, 1479
BspTNI GGTCTC 1 cut(s) 1058
BsrI ACTGG 3 cut(s) 94, 1101, 1206
BssECI CCNNGG 3 cut(s) 171, 357, 1510
BssNAI GTATAC 2 cut(s) 558, 1658
BssSI CACGAG 1 cut(s) 703
BssT1I CCWWGG 3 cut(s) 171, 357, 1510
Bst1107I GTATAC 2 cut(s) 558, 1658
Bst2BI CACGAG 1 cut(s) 703
Bst2UI CCWGG 3 cut(s) 257, 805, 1697
Bst4CI ACNGT 2 cut(s) 1679, 1812
Bst6I CTCTTC 2 cut(s) 497, 762
BstBAI YACGTR 1 cut(s) 1661
BstC8I GCNNGC 4 cut(s) 58, 215, 1367, 1519
BstDEI CTNAG 8 cut(s) 305, 498, 550, 821, 918, 1150, 1378, 1644
BstDSI CCRYGG 2 cut(s) 171, 357
BstEII GGTNACC 1 cut(s) 166
BstENI CCTNNNNNAGG 1 cut(s) 549
BstF5I GGATG 2 cut(s) 813, 817
BstFNI CGCG 1 cut(s) 1408
BstHHI GCGC 1 cut(s) 95
BstMAI GTCTC 5 cut(s) 476, 1058, 1328, 1685, 1760
BstMWI GCNNNNNNNGC 2 cut(s) 669, 1616
BstNI CCWGG 3 cut(s) 257, 805, 1697
BstNSI RCATGY 1 cut(s) 494
BstPAI GACNNNNGTC 1 cut(s) 999
BstPI GGTNACC 1 cut(s) 166
BstSCI CCNGG 3 cut(s) 255, 803, 1695
BstSFI CTRYAG 1 cut(s) 425
BstUI CGCG 1 cut(s) 1408
BstV1I GCAGC 5 cut(s) 1021, 1174, 1427, 1430, 1574
BstV2I GAAGAC 3 cut(s) 620, 860, 1206
BstX2I RGATCY 6 cut(s) 352, 506, 778, 830, 880, 1471
BstYI RGATCY 6 cut(s) 352, 506, 778, 830, 880, 1471
BstZ17I GTATAC 2 cut(s) 558, 1658
BsuI GTATCC 1 cut(s) 1800
BsuRI GGCC 2 cut(s) 1365, 1395
BtgI CCRYGG 2 cut(s) 171, 357
BtgZI GCGATG 1 cut(s) 810
BtsCI GGATG 2 cut(s) 813, 817
BtsI GCAGTG 1 cut(s) 679
BtsIMutI CAGTG 3 cut(s) 87, 679, 1326
Cac8I GCNNGC 4 cut(s) 58, 215, 1367, 1519
CaiI CAGNNNCTG 1 cut(s) 1082
CfoI GCGC 1 cut(s) 95
CseI GACGC 3 cut(s) 1066, 1397, 1753
Csp6I GTAC 1 cut(s) 1709
CviAII CATG 9 cut(s) 172, 286, 358, 491, 592, 665, 1294, 1753, 1800
CviQI GTAC 1 cut(s) 1709
DdeI CTNAG 8 cut(s) 305, 498, 550, 821, 918, 1150, 1378, 1644
DraI TTTAAA 1 cut(s) 1453
EaeI YGGCCR 1 cut(s) 1393
Eam1104I CTCTTC 2 cut(s) 497, 762
EarI CTCTTC 2 cut(s) 497, 762
Eco130I CCWWGG 3 cut(s) 171, 357, 1510
Eco147I AGGCCT 1 cut(s) 1365
Eco31I GGTCTC 1 cut(s) 1058
Eco32I GATATC 1 cut(s) 1758
Eco57I CTGAAG 1 cut(s) 1249
Eco88I CYCGRG 1 cut(s) 1763
Eco91I GGTNACC 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoO65I GGTNACC 1 cut(s) 166
EcoRII CCWGG 3 cut(s) 255, 803, 1695
EcoRV GATATC 1 cut(s) 1758
EcoT14I CCWWGG 3 cut(s) 171, 357, 1510
ErhI CCWWGG 3 cut(s) 171, 357, 1510
Esp3I CGTCTC 1 cut(s) 1328
FaeI CATG 9 cut(s) 175, 289, 361, 494, 595, 668, 1297, 1756, 1803
FaqI GGGAC 1 cut(s) 938
FatI CATG 9 cut(s) 171, 285, 357, 490, 591, 664, 1293, 1752, 1799
FbaI TGATCA 1 cut(s) 1330
FblI GTMKAC 2 cut(s) 557, 1657
Fnu4HI GCNGC 5 cut(s) 1010, 1188, 1416, 1419, 1563
FokI GGATG 2 cut(s) 820, 824
Fsp4HI GCNGC 5 cut(s) 1010, 1188, 1416, 1419, 1563
FspBI CTAG 8 cut(s) 149, 332, 633, 1422, 1437, 1502, 1628, 1706
GlaI GCGC 1 cut(s) 94
GluI GCNGC 5 cut(s) 1010, 1188, 1416, 1419, 1563
HaeIII GGCC 2 cut(s) 1365, 1395
HapII CCGG 1 cut(s) 11
HgaI GACGC 3 cut(s) 1066, 1397, 1753
HhaI GCGC 1 cut(s) 95
Hin1II CATG 9 cut(s) 175, 289, 361, 494, 595, 668, 1297, 1756, 1803
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HincII GTYRAC 2 cut(s) 540, 1087
HindII GTYRAC 2 cut(s) 540, 1087
HindIII AAGCTT 2 cut(s) 54, 1608
HpaI GTTAAC 1 cut(s) 1087
HpaII CCGG 1 cut(s) 11
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 6 cut(s) 540, 558, 1087, 1375, 1429, 1658
Hpy188III TCNNGA 6 cut(s) 77, 149, 633, 776, 878, 1334
Hpy8I GTNNAC 6 cut(s) 540, 558, 1087, 1375, 1429, 1658
HpyAV CCTTC 5 cut(s) 924, 1088, 1130, 1273, 1439
HpyCH4III ACNGT 2 cut(s) 1679, 1812
HpyCH4IV ACGT 2 cut(s) 1321, 1660
HpyCH4V TGCA 7 cut(s) 427, 890, 971, 1009, 1415, 1517, 1667
HpyF10VI GCNNNNNNNGC 2 cut(s) 669, 1616
HpyF3I CTNAG 8 cut(s) 305, 498, 550, 821, 918, 1150, 1378, 1644
HpySE526I ACGT 2 cut(s) 1321, 1660
Hsp92II CATG 9 cut(s) 175, 289, 361, 494, 595, 668, 1297, 1756, 1803
HspAI GCGC 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 1330
KspAI GTTAAC 1 cut(s) 1087
LmnI GCTCC 1 cut(s) 252
Lsp1109I GCAGC 5 cut(s) 1021, 1174, 1427, 1430, 1574
LweI GCATC 5 cut(s) 142, 589, 802, 1040, 1654
MaeI CTAG 8 cut(s) 149, 332, 633, 1422, 1437, 1502, 1628, 1706
MaeII ACGT 2 cut(s) 1321, 1660
MaeIII GTNAC 1 cut(s) 166
MfeI CAATTG 1 cut(s) 1004
MflI RGATCY 6 cut(s) 352, 506, 778, 830, 880, 1471
MlsI TGGCCA 1 cut(s) 1395
MluNI TGGCCA 1 cut(s) 1395
MlyI GAGTC 7 cut(s) 615, 740, 781, 842, 883, 1471, 1762
MmeI TCCRAC 2 cut(s) 330, 1698
Mox20I TGGCCA 1 cut(s) 1395
MroXI GAANNNNTTC 2 cut(s) 640, 947
MscI TGGCCA 1 cut(s) 1395
MseI TTAA 4 cut(s) 120, 533, 1086, 1452
MslI CAYNNNNRTG 2 cut(s) 590, 1311
Msp20I TGGCCA 1 cut(s) 1395
MspA1I CMGCKG 2 cut(s) 1130, 1565
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 3 cut(s) 257, 805, 1697
MunI CAATTG 1 cut(s) 1004
Mva1269I GAATGC 4 cut(s) 217, 715, 973, 1733
MvaI CCWGG 3 cut(s) 257, 805, 1697
MvnI CGCG 1 cut(s) 1408
MwoI GCNNNNNNNGC 2 cut(s) 669, 1616
NcoI CCATGG 2 cut(s) 171, 357
NlaIII CATG 9 cut(s) 175, 289, 361, 494, 595, 668, 1297, 1756, 1803
NlaIV GGNNCC 3 cut(s) 254, 354, 1099
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 1 cut(s) 494
PceI AGGCCT 1 cut(s) 1365
PciI ACATGT 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 792
PctI GAATGC 4 cut(s) 217, 715, 973, 1733
PdmI GAANNNNTTC 2 cut(s) 640, 947
PfeI GAWTC 4 cut(s) 316, 678, 800, 1796
PfoI TCCNGGA 1 cut(s) 803
PkrI GCNGC 5 cut(s) 1011, 1189, 1417, 1420, 1564
PleI GAGTC 7 cut(s) 615, 740, 780, 842, 882, 1470, 1762
PpsI GAGTC 7 cut(s) 615, 740, 780, 842, 882, 1470, 1762
Ppu21I YACGTR 1 cut(s) 1661
PscI ACATGT 1 cut(s) 490
PshAI GACNNNNGTC 1 cut(s) 999
PsiI TTATAA 2 cut(s) 392, 909
Psp6I CCWGG 3 cut(s) 255, 803, 1695
PspEI GGTNACC 1 cut(s) 166
PspGI CCWGG 3 cut(s) 255, 803, 1695
PspN4I GGNNCC 3 cut(s) 254, 354, 1099
PstI CTGCAG 1 cut(s) 429
PstNI CAGNNNCTG 1 cut(s) 1082
PsuI RGATCY 6 cut(s) 352, 506, 778, 830, 880, 1471
PvuII CAGCTG 1 cut(s) 1565
RsaI GTAC 1 cut(s) 1710
RsaNI GTAC 1 cut(s) 1709
RseI CAYNNNNRTG 2 cut(s) 590, 1311
SaqAI TTAA 4 cut(s) 120, 533, 1086, 1452
SatI GCNGC 5 cut(s) 1010, 1188, 1416, 1419, 1563
SchI GAGTC 7 cut(s) 615, 740, 781, 842, 883, 1471, 1762
ScrFI CCNGG 3 cut(s) 257, 805, 1697
SfaNI GCATC 5 cut(s) 142, 589, 802, 1040, 1654
SfcI CTRYAG 1 cut(s) 425
SmiMI CAYNNNNRTG 2 cut(s) 590, 1311
SseBI AGGCCT 1 cut(s) 1365
SsiI CCGC 4 cut(s) 250, 1128, 1589, 1690
SspI AATATT 1 cut(s) 406
SspMI CTAG 8 cut(s) 149, 332, 633, 1422, 1437, 1502, 1628, 1706
StuI AGGCCT 1 cut(s) 1365
StyD4I CCNGG 3 cut(s) 255, 803, 1695
StyI CCWWGG 3 cut(s) 171, 357, 1510
TaaI ACNGT 2 cut(s) 1679, 1812
TaiI ACGT 2 cut(s) 1324, 1663
TaqI TCGA 3 cut(s) 240, 851, 1034
TfiI GAWTC 4 cut(s) 316, 678, 800, 1796
Tru1I TTAA 4 cut(s) 120, 533, 1086, 1452
Tru9I TTAA 4 cut(s) 120, 533, 1086, 1452
TscAI CASTG 3 cut(s) 94, 679, 1333
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 5 cut(s) 1009, 1187, 1415, 1418, 1562
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 5 cut(s) 353, 468, 597, 912, 1160
TspRI CASTG 3 cut(s) 94, 679, 1333
XagI CCTNNNNNAGG 1 cut(s) 549
XapI RAATTY 1 cut(s) 686
XbaI TCTAGA 2 cut(s) 148, 632
XceI RCATGY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 671
XmiI GTMKAC 2 cut(s) 557, 1657
XmnI GAANNNNTTC 2 cut(s) 640, 947
XspI CTAG 8 cut(s) 149, 332, 633, 1422, 1437, 1502, 1628, 1706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.