pycom10g25130

Polyphenol oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005180
Physical Location & Seq
Forward (+)
26785563 .. 26786397
835 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 693 bp
ATGGCTTCTTCTCTTCCACTATCATCCAACACCAAGATTACCACTTGCGCCATCGCCCCTTCACTCCCTCACATCTTCCCAAACAAATCCCAGATCTCCTTAGTCAGAAATCTTAGAGGTTCATGTAAAGCAACAACAATCAACAGTGACCAAAACCTTAATCAAGATCCGCAAACTTGTCTGGTAAAATTGGATAGGAGAAATCTTCTCCTTGGTCTTGGAGGACTTGCCTTGGGGACTCATAACCTAACAAGGCCAGCAAACGCGGCAGAGGCGTCAAAAAAGGTAGTTTTGGTGGGGAGTAGTGAGTTGCCAATAGTTTTGGACTCTGTGGTGAGCGTGATCGTGCCGCGGCCGAGGAGGTCGAGGAGCAAGAAGGACAAGGAGGAGGAAGAGGAGGTGTTGGCGATTGAAGGGATTGAGTTTGTGGCAGACAAGGTGCTCAAGTTCGACGTGCATGTGAACGATGATGAGGACTCGCTGAGTCGACCTGACAAGTCCGAGTTTGCAGGGAGCTTGGTGTTTTTGCCACAGTCCAAGAAAAAGGTTAAAACCAGCCTGCATCTTGGGATTTCGGACTTGCTGGAGGACTTGGGAGCTGACGATGATGGCAGTATCAAGGTAACTTTGGTGCCAAGGAATGTCCAGCGACCTGTCACCATTGGTCGGATCAGGATCGAATATTTTGCCTAG

Protein Analysis

231

Amino Acids

25.05

Weight (kDa)

8.4

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPO1_KFDV PF12143 105 - 228 4.4e-44 Chloroplastic Polyphenol Oxidase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 631
AccI GTMKAC 1 cut(s) 487
AccII CGCG 2 cut(s) 266, 352
AciI CCGC 4 cut(s) 170, 266, 350, 352
AclWI GGATC 3 cut(s) 161, 677, 683
AcoI YGGCCR 1 cut(s) 353
AcyI GRCGYC 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 666
AgsI TTSAA 1 cut(s) 413
AjiI CACGTC 1 cut(s) 454
AluBI AGCT 2 cut(s) 516, 599
AluI AGCT 2 cut(s) 516, 599
Alw21I GWGCWC 1 cut(s) 444
AlwI GGATC 3 cut(s) 161, 677, 683
AoxI GGCC 2 cut(s) 254, 353
AspLEI GCGC 1 cut(s) 50
AsuHPI GGTGA 2 cut(s) 346, 649
BanI GGYRCC 1 cut(s) 631
Bbv12I GWGCWC 1 cut(s) 444
BccI CCATC 2 cut(s) 59, 602
BcgI CGANNNNNNTGC 1 cut(s) 668
BfaI CTAG 1 cut(s) 691
BglII AGATCT 1 cut(s) 93
BisI GCNGC 3 cut(s) 267, 350, 353
BlsI GCNGC 3 cut(s) 268, 351, 354
BmgBI CACGTC 1 cut(s) 454
BmiI GGNNCC 1 cut(s) 633
BmsI GCATC 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 605
BpuEI CTTGAG 1 cut(s) 428
BsaBI GATNNNNATC 1 cut(s) 674
BsaHI GRCGYC 1 cut(s) 275
BsaJI CCNNGG 5 cut(s) 211, 231, 350, 356, 635
Bsc4I CCNNNNNNNGG 1 cut(s) 666
Bse8I GATNNNNATC 1 cut(s) 674
BseDI CCNNGG 5 cut(s) 211, 231, 350, 356, 635
BseGI GGATG 1 cut(s) 23
BseJI GATNNNNATC 1 cut(s) 674
BseLI CCNNNNNNNGG 1 cut(s) 666
BseMII CTCAG 1 cut(s) 473
BseRI GAGGAG 4 cut(s) 373, 382, 401, 410
BseX3I CGGCCG 1 cut(s) 353
Bsh1236I CGCG 2 cut(s) 266, 352
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 2 cut(s) 256, 355
BshNI GGYRCC 1 cut(s) 631
BsiEI CGRYCG 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 444
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 2 cut(s) 256, 355
Bsp1286I GDGCHC 1 cut(s) 444
Bsp143I GATC 5 cut(s) 93, 166, 342, 669, 675
BspACI CCGC 4 cut(s) 170, 266, 350, 352
BspANI GGCC 2 cut(s) 256, 355
BspCNI CTCAG 1 cut(s) 474
BspFNI CGCG 2 cut(s) 266, 352
BspLI GGNNCC 1 cut(s) 633
BspPI GGATC 3 cut(s) 161, 677, 683
BspT107I GGYRCC 1 cut(s) 631
BssECI CCNNGG 5 cut(s) 211, 231, 350, 356, 635
BssMI GATC 5 cut(s) 93, 166, 342, 669, 675
BssNI GRCGYC 1 cut(s) 275
BssT1I CCWWGG 3 cut(s) 211, 231, 635
Bst4CI ACNGT 2 cut(s) 146, 534
Bst6I CTCTTC 2 cut(s) 18, 387
BstACI GRCGYC 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 258, 560
BstDEI CTNAG 3 cut(s) 100, 113, 482
BstDSI CCRYGG 1 cut(s) 350
BstF5I GGATG 1 cut(s) 23
BstFNI CGCG 2 cut(s) 266, 352
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 5 cut(s) 96, 169, 345, 672, 678
BstMBI GATC 5 cut(s) 93, 166, 342, 669, 675
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 2 cut(s) 266, 272
BstNSI RCATGY 1 cut(s) 461
BstUI CGCG 2 cut(s) 266, 352
BstX2I RGATCY 2 cut(s) 93, 166
BstYI RGATCY 2 cut(s) 93, 166
BstZI CGGCCG 1 cut(s) 353
BsuRI GGCC 2 cut(s) 256, 355
BtgI CCRYGG 1 cut(s) 350
BtgZI GCGATG 1 cut(s) 37
BtrI CACGTC 1 cut(s) 454
BtsCI GGATG 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 2 cut(s) 258, 560
CfoI GCGC 1 cut(s) 50
Cfr42I CCGCGG 1 cut(s) 353
CseI GACGC 1 cut(s) 264
CviAII CATG 2 cut(s) 123, 458
CviJI RGCY 6 cut(s) 5, 256, 355, 516, 558, 599
CviKI_1 RGCY 6 cut(s) 5, 256, 355, 516, 558, 599
DdeI CTNAG 3 cut(s) 100, 113, 482
DpnI GATC 5 cut(s) 95, 168, 344, 671, 677
DpnII GATC 5 cut(s) 93, 166, 342, 669, 675
EaeI YGGCCR 1 cut(s) 353
EagI CGGCCG 1 cut(s) 353
Eam1104I CTCTTC 2 cut(s) 18, 387
EarI CTCTTC 2 cut(s) 18, 387
EclXI CGGCCG 1 cut(s) 353
Eco130I CCWWGG 3 cut(s) 211, 231, 635
Eco52I CGGCCG 1 cut(s) 353
EcoT14I CCWWGG 3 cut(s) 211, 231, 635
ErhI CCWWGG 3 cut(s) 211, 231, 635
FaeI CATG 2 cut(s) 126, 461
FaiI YATR 3 cut(s) 124, 243, 459
FaqI GGGAC 1 cut(s) 250
FatI CATG 2 cut(s) 122, 457
FblI GTMKAC 1 cut(s) 487
Fnu4HI GCNGC 3 cut(s) 267, 350, 353
FokI GGATG 1 cut(s) 10
Fsp4HI GCNGC 3 cut(s) 267, 350, 353
FspBI CTAG 1 cut(s) 691
GlaI GCGC 1 cut(s) 49
GluI GCNGC 3 cut(s) 267, 350, 353
GsuI CTGGAG 1 cut(s) 605
HaeIII GGCC 2 cut(s) 256, 355
HgaI GACGC 1 cut(s) 264
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 1 cut(s) 275
Hin1II CATG 2 cut(s) 126, 461
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HincII GTYRAC 1 cut(s) 488
HindII GTYRAC 1 cut(s) 488
HinfI GANTC 4 cut(s) 238, 326, 476, 484
HphI GGTGA 2 cut(s) 346, 649
Hpy166II GTNNAC 2 cut(s) 463, 488
Hpy188I TCNGA 4 cut(s) 107, 502, 577, 669
Hpy188III TCNNGA 2 cut(s) 164, 673
Hpy8I GTNNAC 2 cut(s) 463, 488
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 3 cut(s) 69, 370, 407
HpyCH4III ACNGT 2 cut(s) 146, 534
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 3 cut(s) 457, 509, 562
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 272
HpyF3I CTNAG 3 cut(s) 100, 113, 482
HpySE526I ACGT 1 cut(s) 453
Hsp92I GRCGYC 1 cut(s) 275
Hsp92II CATG 2 cut(s) 126, 461
HspAI GCGC 1 cut(s) 48
KspI CCGCGG 1 cut(s) 353
Kzo9I GATC 5 cut(s) 93, 166, 342, 669, 675
LmnI GCTCC 3 cut(s) 369, 513, 596
LweI GCATC 1 cut(s) 571
MaeI CTAG 1 cut(s) 691
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 3 cut(s) 146, 622, 655
MalI GATC 5 cut(s) 95, 168, 344, 671, 677
MboI GATC 5 cut(s) 93, 166, 342, 669, 675
MboII GAAGA 4 cut(s) 5, 67, 197, 404
MflI RGATCY 2 cut(s) 93, 166
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 1 cut(s) 188
MlyI GAGTC 4 cut(s) 232, 320, 470, 493
MmeI TCCRAC 2 cut(s) 51, 647
MseI TTAA 2 cut(s) 159, 549
MspA1I CMGCKG 1 cut(s) 352
MvnI CGCG 2 cut(s) 266, 352
MwoI GCNNNNNNNGC 2 cut(s) 266, 272
NdeII GATC 5 cut(s) 93, 166, 342, 669, 675
NlaIII CATG 2 cut(s) 126, 461
NlaIV GGNNCC 1 cut(s) 633
NmeAIII GCCGAG 1 cut(s) 381
NmuCI GTSAC 2 cut(s) 146, 655
NspI RCATGY 1 cut(s) 461
PkrI GCNGC 3 cut(s) 268, 351, 354
PleI GAGTC 4 cut(s) 232, 320, 470, 492
PpsI GAGTC 4 cut(s) 232, 320, 470, 492
PspN4I GGNNCC 1 cut(s) 633
PsuI RGATCY 2 cut(s) 93, 166
SacII CCGCGG 1 cut(s) 353
SalI GTCGAC 1 cut(s) 486
SaqAI TTAA 2 cut(s) 159, 549
SatI GCNGC 3 cut(s) 267, 350, 353
Sau3AI GATC 5 cut(s) 93, 166, 342, 669, 675
SchI GAGTC 4 cut(s) 232, 320, 470, 493
SduI GDGCHC 1 cut(s) 444
SfaNI GCATC 1 cut(s) 571
Sfr303I CCGCGG 1 cut(s) 353
SgrBI CCGCGG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 1 cut(s) 188
SsiI CCGC 4 cut(s) 170, 266, 350, 352
SspI AATATT 1 cut(s) 683
SspMI CTAG 1 cut(s) 691
StyI CCWWGG 3 cut(s) 211, 231, 635
TaaI ACNGT 2 cut(s) 146, 534
TaiI ACGT 1 cut(s) 456
TaqI TCGA 4 cut(s) 365, 450, 487, 678
TasI AATT 1 cut(s) 188
TauI GCSGC 3 cut(s) 269, 352, 355
Tru1I TTAA 2 cut(s) 159, 549
Tru9I TTAA 2 cut(s) 159, 549
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 2 cut(s) 146, 655
Tsp45I GTSAC 2 cut(s) 146, 655
TspDTI ATGAA 1 cut(s) 111
TspRI CASTG 1 cut(s) 151
XceI RCATGY 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 487
XspI CTAG 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.