Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007796

Physical Location & Seq

Genomic Coordinates Reverse (-)

28874610 .. 28879333

Gene Structure 4724 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2766 bp

ATGTCCATCACCGGGAGCCCCACCACCAGCAGCACCGTCGATTGCGCTGCCTACCAGCGCAGCGACACCACTGCCCTAACTGCCGTCCCCGCCGCGGTGGATCAGTTCCACCACCGATTGTCGTCCAAGCGCAAACTCGACGACTATGGCGGGCCCACCTTCTCCGACGAAGAGGACGACGCCCTTCTCTCTGACCTAGTCCACGTCAGGATGAGGAAGGACGAACCCAACGCCATCGATTCGTCGTCCAACGGCGTCGCCAACCTCCCTCACTCCTCCGCCAACAGCGACGCCCTCAATCCCCGTGTTCCCAATGCCCGATCGGCCTCTCACGGAGAGTCCGCTCACTCCGAGTCGACTCGGTCCCGTACCATGCTCCAATTCTTCATCAGAACCATGTCCGGTGGCAATAATCTGGTAATCCAGGCCTACGCTGACGACAAGGTGAAATCGCTACACCATCGCATACAGACGATCACTGGCATTCCGGTTTTTGAGCAAAGGCTTATATATAGAGGTAAACAGCTGCAGCCGGACCAGTCGCTCGCTGAGTGCGCAATCCAAAACGACGCCAGTTTGCAACTGGTCGGCCGCTTGCGGAGCACCGACCACCCTCAGGCCTGGCAACTCCTGGAGGACATAGTCTCCGCCGCATTTCGGATGTGCCGAGGTGAAGAACTTCACGAGCCGTCCAAATACATCAAGAGCAGGATGAGCCAGTACTTAACCATGGCTCAGAAGGAGATGACAGCAGGATATGGGGTTAGCCATATCCGGGTGTTCCTTCCGTCCTCTGCCCCCTTGGCGCTGCTGATGCTCTATGTTTCTCCCCTGCCAGCTAACAGAACAGTTGCTGAAAATTCAATCAAGTATTTCCTGAATTCGTACCCTACATTGTTGCCGAAACACTTGCATAATTACTGCGCGCCGATTGTGTTAGAGTTTTGTAAGTTTCTTAGGAGATTAGGGGAGGAGGACCCTCTGTACCTCCTGTGCCGAGGTTCACTTGGTTCCTTGTTAGAAAGTGATGGAAATTCGCAGGACTCGGAGCCAGTGGAGGGATTGATTGGTGGGTTGAAAGAGATTTCTCCATTTGTTAGGGAGCTGTCAAGTATTTTGTCTAGGGACTTGGTAGTGAGTATGGACCTTCCTGCGAGTGGCCGGCCGGTGTCGGATGATGTACGTGATTTTAGAGCGTTCTTGCTCCCTTTGCGCACTGCAGTTGCCAGTCAAGTGTGTTTCAAGAGTTCTAATCTTGCATCCTCGAAAGGGGGTACTTTTAGGCATCCACTATATGGAGAGGAGATTGAGCTTCTTCACGTTATATATGCTGATTTGCTGAAAAAAATGGATGAATGCCTTGGAAAAATGGATGAGTCTTTGGCTGGGAAAGGAAAGGTGGAAGGTGATGTTACTCATTCTGGATGGTCTCAGTATCTTGCTGTTTTAAAGGAACTGAGTGGAATTTCTATGCTTTATCAGAGCTGGGAAGAACAATTTAAGACGATTTTGAGGCTTAGAAGGGCTGCATTATGTGCTTTAGTTGTCAAGTATGCAATGCGAAGTGATGATCACCAGTGGCTTATTAAGCACAAAGATGTGCTAGATTTTGAGTCTCGGAGGCATTTGGCTATGATGATGTTCCCTGATGTTAAAGAAGACTATGAGGAGCTGCATGAGATGCTCATTGATAGGTCGCAAATATTGGCAGAATCGTTTGAGTACATAGGGCGTGCGGAACCTGAGTTACTTCATGGCGGTTTATTTATGGAATTCAAAAATGAGGAAGCTACAGGTCCAGGTGTACTGCGTGAGTGGTTCTTTTTGGTATGCCAGGCCATATTTAATCCGCAAAATGCACTTTTTGTTGCATGCCCAAACGACTGTAGAAGGTTCTATCCTAATCCAGCATCTAAAGTTGATCGTCTGCACCTTGAGTATTTCACCTTTGCTGGCCGAGTGATTGCATTAGCTTTGATGCATAAAGTGCAGGTGGGCATAGTCTTTGATCGTGTGTTTTTCCAGCAACTGGCTGGAGATATATATTTATCTTTGGAAGACATAAGAGATGCAGATCCAGTCTTGTATAATAGTTGCAAGAAGATACTAGAAATGGATGCCAAGTCTATTGATTCAGATGAGTTAGGGTTAACATTTGTTAGAGAAGTGGAGGAGTTAGGATTCCGGAAAACTGTGGAGCTTTGCCCTGCTGGGAAAAGCATTGTTGTGAACAGCAAGAACAGGGGGGAATACATTAATCTTCTAATTCAGCATCGTTTTGTCACATCTATATCTGAACAGGTGTCCCACTTTGCGCAAGGTTTTGCTGATATACTTTGTGGCTCAAGGCTCCAGAGCATCTTTTTCCGAAGTTTAGAGCTAGAAGATCTTGATTGGATGCTTCACGGAAGTGAAAGTGCTATTAGTGTTGAAGACTGGAAGGCGCATACTGATTACAATGGCTATAAAGAGACCGATCCTCAAATACTCTGGTTCTGGAAGATTGTTGGGAAAATGTCTGCAGAGCAGAAAAAGGTTCTTCTCTTCTTTTGGACCTCGGTGAAGTATCTTCCAGTAGAGGGGTTCCGAGGTTTGGCTTCTCGGCTTTACATTTACAAGTCCTCAGAGGCTTGCAGTCGCCTGCCTTCGTCTCATACTTGCTTTTACCGGCTGTGTTTCCCGCCTTATCCATGGGAGGCAGAGATGAAAAGCCGCCTTGACATCATCACCCAAGAACATGTCGGCAGTAGCTTCGGCACATGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009636 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0010033 GO:0010035 GO:0010150 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046677 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0090693 GO:0099402 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04011 ko04120 ko04144 ko04530 ko05169 map04011 map04120 map04144 map04530 map05169

Pfam Domains

Protein Families

HECT ubiquitin

Protein Analysis

922

Amino Acids

104.14

Weight (kDa)

5.93

Isoelectric Point (pI)

47.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ubiquitin	PF00240	129 - 200	5.3e-16	Ubiquitin family
HECT	PF00632	609 - 913	1e-76	HECT-domain (ubiquitin-transferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007796)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G12570
fragaria_vesca	FvH4_3g00910 FvH4_3g00910
malus_domestica	MD05G1358400.v1.1 MD10G1332500.v1.1
prunus_persica	Prupe.4G009700_v2.0.a1 Prupe.4G009700_v2.0.a1
pyrus_communis	pycom05g32410 pycom10g28190
rosa_chinensis	RchiOBHm_Chr5g0001411 RchiOBHm_Chr5g0001431
rosa_laevigata	RLG00000030927
rosa_multiflora	Rmu_sc0003749.1_g000014
rosa_roxburghii	Rroxscaffold_1G00074960 Rroxscaffold_1G00074970
rosa_rugosa	Rorug04G0390300
rosa_samantha	Rh5AG010900 Rh5AG011000 Rh5BG013500 Rh5CG011900 Rh5CG012000 Rh5DG011200 Rh7AG192400
rosa_wichuraiana	Rw5G001040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1981
Acc16I	TGCGCA	3 cut(s)	556, 1214, 2316
Acc36I	ACCTGC	1 cut(s)	1981
AccB7I	CCANNNNNTGG	2 cut(s)	1295, 2029
AccBSI	CCGCTC	1 cut(s)	344
AccI	GTMKAC	1 cut(s)	356
AccII	CGCG	2 cut(s)	95, 926
AccIII	TCCGGA	1 cut(s)	2184
AclWI	GGATC	3 cut(s)	108, 2069, 2471
AcoI	YGGCCR	4 cut(s)	589, 1159, 1163, 1954
AcsI	RAATTY	5 cut(s)	859, 880, 1033, 1464, 1772
AcyI	GRCGYC	4 cut(s)	180, 255, 291, 570
AfaI	GTAC	8 cut(s)	370, 722, 887, 986, 1182, 1276, 1724, 1806
AflIII	ACRYGT	1 cut(s)	2737
AgsI	TTSAA	5 cut(s)	864, 1078, 1243, 1777, 2432
AjiI	CACGTC	1 cut(s)	205
AjnI	CCWGG	5 cut(s)	423, 620, 630, 1798, 1833
AleI	CACNNNNGTG	1 cut(s)	2409
Alw21I	GWGCWC	1 cut(s)	605
Alw26I	GTCTC	5 cut(s)	649, 1434, 1620, 2465, 2655
AlwI	GGATC	3 cut(s)	108, 2069, 2471
AlwNI	CAGNNNCTG	2 cut(s)	854, 2029
Aor13HI	TCCGGA	1 cut(s)	2184
AoxI	GGCC	9 cut(s)	152, 324, 426, 589, 618, 1159, 1163, 1836, 1954
ApaI	GGGCCC	1 cut(s)	156
ApeKI	GCWGC	8 cut(s)	30, 47, 60, 526, 529, 808, 1526, 1672
ApoI	RAATTY	5 cut(s)	859, 880, 1033, 1464, 1772
AseI	ATTAAT	1 cut(s)	2256
Asp700I	GAANNNNTTC	1 cut(s)	678
AspS9I	GGNCC	8 cut(s)	152, 153, 363, 535, 976, 1144, 1796, 2553
AsuC2I	CCSGG	2 cut(s)	13, 776
AsuHPI	GGTGA	7 cut(s)	457, 683, 1418, 1565, 1936, 2572, 2719
AvaII	GGWCC	6 cut(s)	363, 535, 976, 1144, 1796, 2553
AxyI	CCTNAGG	1 cut(s)	615
BaeGI	GKGCMC	1 cut(s)	156
BanII	GRGCYC	2 cut(s)	20, 156
BauI	CACGAG	1 cut(s)	683
BbsI	GAAGAC	3 cut(s)	1665, 2064, 2439
Bbv12I	GWGCWC	1 cut(s)	605
BbvI	GCAGC	8 cut(s)	34, 42, 72, 513, 541, 795, 1513, 1659
BccI	CCATC	5 cut(s)	14, 242, 468, 1022, 1419
BceAI	ACGGC	3 cut(s)	68, 268, 673
BcgI	CGANNNNNNTGC	9 cut(s)	19, 29, 53, 53, 63, 87, 892, 926, 2734
BciT130I	CCWGG	5 cut(s)	425, 622, 632, 1800, 1835
BclI	TGATCA	1 cut(s)	1570
BcnI	CCSGG	2 cut(s)	13, 776
BcoDI	GTCTC	5 cut(s)	649, 1434, 1620, 2465, 2655
BfaI	CTAG	5 cut(s)	197, 1122, 1604, 2108, 2381
BfmI	CTRYAG	5 cut(s)	527, 1218, 1791, 1885, 2520
BfoI	RGCGCY	1 cut(s)	809
BfuAI	ACCTGC	1 cut(s)	1981
BglI	GCCNNNNNGGC	2 cut(s)	323, 803
BglII	AGATCT	1 cut(s)	2386
BmcAI	AGTACT	1 cut(s)	722
Bme1390I	CCNGG	7 cut(s)	13, 425, 622, 632, 776, 1800, 1835
Bme18I	GGWCC	6 cut(s)	363, 535, 976, 1144, 1796, 2553
BmgBI	CACGTC	1 cut(s)	205
BmgT120I	GGNCC	8 cut(s)	152, 153, 363, 535, 976, 1144, 1796, 2553
BmiI	GGNNCC	9 cut(s)	17, 154, 365, 978, 1012, 1050, 1740, 2351, 2585
BmrFI	CCNGG	7 cut(s)	13, 425, 622, 632, 776, 1800, 1835
BpiI	GAAGAC	3 cut(s)	1665, 2064, 2439
BpmI	CTGGAG	3 cut(s)	653, 2055, 2336
BpuEI	CTTGAG	2 cut(s)	1955, 2329
BpuMI	CCSGG	2 cut(s)	13, 776
Bsa29I	ATCGAT	1 cut(s)	237
BsaAI	YACGTR	1 cut(s)	1184
BsaBI	GATNNNNATC	1 cut(s)	2073
BsaHI	GRCGYC	4 cut(s)	180, 255, 291, 570
BsaI	GGTCTC	2 cut(s)	1434, 2465
BsaJI	CCNNGG	9 cut(s)	93, 667, 729, 801, 997, 1360, 2556, 2587, 2690
BsaWI	WCCGGW	3 cut(s)	401, 487, 2184
BsaXI	ACNNNNNCTCC	2 cut(s)	629, 659
Bse118I	RCCGGY	3 cut(s)	1161, 1165, 2667
Bse21I	CCTNAGG	1 cut(s)	615
Bse3DI	GCAATG	1 cut(s)	1563
Bse8I	GATNNNNATC	1 cut(s)	2073
BseAI	TCCGGA	1 cut(s)	2184
BseBI	CCWGG	5 cut(s)	425, 622, 632, 1800, 1835
BseCI	ATCGAT	1 cut(s)	237
BseDI	CCNNGG	9 cut(s)	93, 667, 729, 801, 997, 1360, 2556, 2587, 2690
BseJI	GATNNNNATC	1 cut(s)	2073
BseMI	GCAATG	1 cut(s)	1563
BseMII	CTCAG	7 cut(s)	540, 629, 749, 1445, 1448, 1734, 2637
BsePI	GCGCGC	1 cut(s)	924
BseRI	GAGGAG	5 cut(s)	265, 986, 1316, 1682, 2186
BseSI	GKGCMC	1 cut(s)	156
BseX3I	CGGCCG	2 cut(s)	589, 1163
BseXI	GCAGC	8 cut(s)	34, 42, 72, 513, 541, 795, 1513, 1659
BseYI	CCCAGC	3 cut(s)	1385, 1485, 2210
BsgI	GTGCAG	2 cut(s)	1913, 2009
Bsh1236I	CGCG	2 cut(s)	95, 926
Bsh1285I	CGRYCG	3 cut(s)	323, 592, 1166
BshFI	GGCC	9 cut(s)	154, 326, 428, 591, 620, 1161, 1165, 1838, 1956
BshVI	ATCGAT	1 cut(s)	237
BsiEI	CGRYCG	3 cut(s)	323, 592, 1166
BsiHKAI	GWGCWC	1 cut(s)	605
BsiSI	CCGG	9 cut(s)	12, 402, 488, 533, 775, 1162, 1166, 2185, 2668
BslFI	GGGAC	4 cut(s)	71, 349, 1139, 2290
BsmAI	GTCTC	5 cut(s)	649, 1434, 1620, 2465, 2655
BsmBI	CGTCTC	1 cut(s)	2655
BsmFI	GGGAC	4 cut(s)	71, 349, 1139, 2290
BsmI	GAATGC	2 cut(s)	483, 1361
BsnI	GGCC	9 cut(s)	154, 326, 428, 591, 620, 1161, 1165, 1838, 1956
Bso31I	GGTCTC	2 cut(s)	1434, 2465
Bsp120I	GGGCCC	1 cut(s)	152
Bsp1286I	GDGCHC	3 cut(s)	20, 156, 605
Bsp13I	TCCGGA	1 cut(s)	2184
Bsp143I	GATC	9 cut(s)	100, 320, 474, 1570, 1921, 2008, 2074, 2386, 2476
Bsp19I	CCATGG	2 cut(s)	729, 2690
BspANI	GGCC	9 cut(s)	154, 326, 428, 591, 620, 1161, 1165, 1838, 1956
BspCNI	CTCAG	7 cut(s)	541, 628, 748, 1444, 1449, 1735, 2636
BspDI	ATCGAT	1 cut(s)	237
BspEI	TCCGGA	1 cut(s)	2184
BspFNI	CGCG	2 cut(s)	95, 926
BspLI	GGNNCC	9 cut(s)	17, 154, 365, 978, 1012, 1050, 1740, 2351, 2585
BspMAI	CTGCAG	3 cut(s)	531, 1222, 2524
BspMI	ACCTGC	1 cut(s)	1981
BspPI	GGATC	3 cut(s)	108, 2069, 2471
BspTNI	GGTCTC	2 cut(s)	1434, 2465
BsrBI	CCGCTC	1 cut(s)	344
BsrDI	GCAATG	1 cut(s)	1563
BsrFI	RCCGGY	3 cut(s)	1161, 1165, 2667
BssAI	RCCGGY	3 cut(s)	1161, 1165, 2667
BssECI	CCNNGG	9 cut(s)	93, 667, 729, 801, 997, 1360, 2556, 2587, 2690
BssHII	GCGCGC	1 cut(s)	924
BssMI	GATC	9 cut(s)	100, 320, 474, 1570, 1921, 2008, 2074, 2386, 2476
BssNI	GRCGYC	4 cut(s)	180, 255, 291, 570
BssSI	CACGAG	1 cut(s)	683
BssT1I	CCWWGG	4 cut(s)	729, 801, 1360, 2690
Bst2BI	CACGAG	1 cut(s)	683
Bst2UI	CCWGG	5 cut(s)	425, 622, 632, 1800, 1835
Bst4CI	ACNGT	4 cut(s)	37, 850, 1886, 2194
Bst6I	CTCTTC	2 cut(s)	165, 2549
BstACI	GRCGYC	4 cut(s)	180, 255, 291, 570
BstAPI	GCANNNNNTGC	3 cut(s)	1535, 1681, 1987
BstBAI	YACGTR	1 cut(s)	1184
BstDEI	CTNAG	9 cut(s)	549, 615, 735, 956, 1431, 1457, 1517, 1743, 2623
BstDSI	CCRYGG	3 cut(s)	93, 729, 2690
BstFNI	CGCG	2 cut(s)	95, 926
BstH2I	RGCGCY	1 cut(s)	809
BstKTI	GATC	9 cut(s)	103, 323, 477, 1573, 1924, 2011, 2077, 2389, 2479
BstMAI	GTCTC	5 cut(s)	649, 1434, 1620, 2465, 2655
BstMBI	GATC	9 cut(s)	100, 320, 474, 1570, 1921, 2008, 2074, 2386, 2476
BstMCI	CGRYCG	3 cut(s)	323, 592, 1166
BstNI	CCWGG	5 cut(s)	425, 622, 632, 1800, 1835
BstNSI	RCATGY	2 cut(s)	1875, 2741
BstSCI	CCNGG	7 cut(s)	11, 423, 620, 630, 774, 1798, 1833
BstSFI	CTRYAG	5 cut(s)	527, 1218, 1791, 1885, 2520
BstSLI	GKGCMC	1 cut(s)	156
BstUI	CGCG	2 cut(s)	95, 926
BstV1I	GCAGC	8 cut(s)	34, 42, 72, 513, 541, 795, 1513, 1659
BstV2I	GAAGAC	3 cut(s)	1665, 2064, 2439
BstX2I	RGATCY	2 cut(s)	2074, 2386
BstYI	RGATCY	2 cut(s)	2074, 2386
BstZI	CGGCCG	2 cut(s)	589, 1163
Bsu15I	ATCGAT	1 cut(s)	237
Bsu36I	CCTNAGG	1 cut(s)	615
BsuRI	GGCC	9 cut(s)	154, 326, 428, 591, 620, 1161, 1165, 1838, 1956
BsuTUI	ATCGAT	1 cut(s)	237
BtgI	CCRYGG	3 cut(s)	93, 729, 2690
BtgZI	GCGATG	1 cut(s)	446
BtrI	CACGTC	1 cut(s)	205
BtsI	GCAGTG	2 cut(s)	69, 1215
BtsIMutI	CAGTG	5 cut(s)	69, 477, 1059, 1215, 1583
BveI	ACCTGC	1 cut(s)	1981
CaiI	CAGNNNCTG	2 cut(s)	854, 2029
Cfr10I	RCCGGY	3 cut(s)	1161, 1165, 2667
Cfr13I	GGNCC	8 cut(s)	152, 153, 363, 535, 976, 1144, 1796, 2553
Cfr42I	CCGCGG	1 cut(s)	96
ClaI	ATCGAT	1 cut(s)	237
CseI	GACGC	4 cut(s)	188, 244, 299, 578
Csp6I	GTAC	8 cut(s)	369, 721, 886, 985, 1181, 1275, 1723, 1805
CviAII	CATG	9 cut(s)	373, 397, 730, 1676, 1754, 1872, 2691, 2738, 2760
CviQI	GTAC	8 cut(s)	369, 721, 886, 985, 1181, 1275, 1723, 1805
DdeI	CTNAG	9 cut(s)	549, 615, 735, 956, 1431, 1457, 1517, 1743, 2623
DpnI	GATC	9 cut(s)	102, 322, 476, 1572, 1923, 2010, 2076, 2388, 2478
DpnII	GATC	9 cut(s)	100, 320, 474, 1570, 1921, 2008, 2074, 2386, 2476
DraI	TTTAAA	1 cut(s)	1449
EaeI	YGGCCR	4 cut(s)	589, 1159, 1163, 1954
EagI	CGGCCG	2 cut(s)	589, 1163
Eam1104I	CTCTTC	2 cut(s)	165, 2549
EarI	CTCTTC	2 cut(s)	165, 2549
EciI	GGCGGA	2 cut(s)	268, 637
EclXI	CGGCCG	2 cut(s)	589, 1163
Eco130I	CCWWGG	4 cut(s)	729, 801, 1360, 2690
Eco147I	AGGCCT	2 cut(s)	428, 620
Eco24I	GRGCYC	2 cut(s)	20, 156
Eco31I	GGTCTC	2 cut(s)	1434, 2465
Eco47I	GGWCC	6 cut(s)	363, 535, 976, 1144, 1796, 2553
Eco52I	CGGCCG	2 cut(s)	589, 1163
Eco81I	CCTNAGG	1 cut(s)	615
EcoO109I	RGGNCCY	1 cut(s)	976
EcoRI	GAATTC	2 cut(s)	880, 1772
EcoRII	CCWGG	5 cut(s)	423, 620, 630, 1798, 1833
EcoT14I	CCWWGG	4 cut(s)	729, 801, 1360, 2690
EcoT22I	ATGCAT	1 cut(s)	1983
EcoT38I	GRGCYC	2 cut(s)	20, 156
ErhI	CCWWGG	4 cut(s)	729, 801, 1360, 2690
Esp3I	CGTCTC	1 cut(s)	2655
FaeI	CATG	9 cut(s)	376, 400, 733, 1679, 1757, 1875, 2694, 2741, 2763
FaqI	GGGAC	4 cut(s)	71, 349, 1139, 2290
FatI	CATG	9 cut(s)	372, 396, 729, 1675, 1753, 1871, 2690, 2737, 2759
FauI	CCCGC	3 cut(s)	97, 143, 2688
FbaI	TGATCA	1 cut(s)	1570
FblI	GTMKAC	1 cut(s)	356
FriOI	GRGCYC	2 cut(s)	20, 156
FseI	GGCCGGCC	1 cut(s)	1165
FspBI	CTAG	5 cut(s)	197, 1122, 1604, 2108, 2381
FspI	TGCGCA	3 cut(s)	556, 1214, 2316
GsaI	CCCAGC	3 cut(s)	1389, 1489, 2214
GsuI	CTGGAG	3 cut(s)	653, 2055, 2336
HaeII	RGCGCY	1 cut(s)	809
HaeIII	GGCC	9 cut(s)	154, 326, 428, 591, 620, 1161, 1165, 1838, 1956
HapII	CCGG	9 cut(s)	12, 402, 488, 533, 775, 1162, 1166, 2185, 2668
HgaI	GACGC	4 cut(s)	188, 244, 299, 578
Hin1I	GRCGYC	4 cut(s)	180, 255, 291, 570
Hin1II	CATG	9 cut(s)	376, 400, 733, 1679, 1757, 1875, 2694, 2741, 2763
HincII	GTYRAC	2 cut(s)	357, 2151
HindII	GTYRAC	2 cut(s)	357, 2151
HpaI	GTTAAC	1 cut(s)	2151
HpaII	CCGG	9 cut(s)	12, 402, 488, 533, 775, 1162, 1166, 2185, 2668
HphI	GGTGA	7 cut(s)	457, 683, 1418, 1565, 1936, 2572, 2719
Hpy166II	GTNNAC	7 cut(s)	202, 357, 521, 1004, 1805, 2151, 2230
Hpy8I	GTNNAC	7 cut(s)	202, 357, 521, 1004, 1805, 2151, 2230
Hpy99I	CGWCG	8 cut(s)	41, 143, 170, 182, 247, 260, 293, 572
HpyCH4III	ACNGT	4 cut(s)	37, 850, 1886, 2194
HpyCH4IV	ACGT	3 cut(s)	204, 1183, 1320
HpyF3I	CTNAG	9 cut(s)	549, 615, 735, 956, 1431, 1457, 1517, 1743, 2623
HpySE526I	ACGT	3 cut(s)	204, 1183, 1320
Hsp92I	GRCGYC	4 cut(s)	180, 255, 291, 570
Hsp92II	CATG	9 cut(s)	376, 400, 733, 1679, 1757, 1875, 2694, 2741, 2763
Kpn2I	TCCGGA	1 cut(s)	2184
KroI	GCCGGC	1 cut(s)	1161
KroNI	GCCGGC	1 cut(s)	1163
Ksp22I	TGATCA	1 cut(s)	1570
KspAI	GTTAAC	1 cut(s)	2151
KspI	CCGCGG	1 cut(s)	96
Kzo9I	GATC	9 cut(s)	100, 320, 474, 1570, 1921, 2008, 2074, 2386, 2476
LmnI	GCTCC	9 cut(s)	15, 381, 600, 1048, 1102, 1209, 1669, 2197, 2355
Lsp1109I	GCAGC	8 cut(s)	34, 42, 72, 513, 541, 795, 1513, 1659
MaeI	CTAG	5 cut(s)	197, 1122, 1604, 2108, 2381
MaeII	ACGT	3 cut(s)	204, 1183, 1320
MaeIII	GTNAC	3 cut(s)	1411, 1746, 2281
MalI	GATC	9 cut(s)	102, 322, 476, 1572, 1923, 2010, 2076, 2388, 2478
MbiI	CCGCTC	1 cut(s)	344
MboI	GATC	9 cut(s)	100, 320, 474, 1570, 1921, 2008, 2074, 2386, 2476
MflI	RGATCY	2 cut(s)	2074, 2386
MhlI	GDGCHC	3 cut(s)	20, 156, 605
MluCI	AATT	9 cut(s)	380, 859, 880, 916, 1033, 1464, 1496, 1772, 2265
MlyI	GAGTC	6 cut(s)	347, 352, 362, 1037, 1385, 1622
MmeI	TCCRAC	3 cut(s)	189, 273, 1152
Mph1103I	ATGCAT	1 cut(s)	1983
MroI	TCCGGA	1 cut(s)	2184
MroNI	GCCGGC	1 cut(s)	1161
MroXI	GAANNNNTTC	1 cut(s)	678
MseI	TTAA	8 cut(s)	725, 1448, 1500, 1587, 1653, 1845, 2150, 2256
MslI	CAYNNNNRTG	3 cut(s)	1596, 2225, 2409
MspA1I	CMGCKG	2 cut(s)	95, 526
MspI	CCGG	9 cut(s)	12, 402, 488, 533, 775, 1162, 1166, 2185, 2668
MspR9I	CCNGG	7 cut(s)	13, 425, 622, 632, 776, 1800, 1835
Mva1269I	GAATGC	2 cut(s)	483, 1361
MvaI	CCWGG	5 cut(s)	425, 622, 632, 1800, 1835
MvnI	CGCG	2 cut(s)	95, 926
NaeI	GCCGGC	1 cut(s)	1163
NciI	CCSGG	2 cut(s)	13, 776
NcoI	CCATGG	2 cut(s)	729, 2690
NdeII	GATC	9 cut(s)	100, 320, 474, 1570, 1921, 2008, 2074, 2386, 2476
NgoMIV	GCCGGC	1 cut(s)	1161
NlaIII	CATG	9 cut(s)	376, 400, 733, 1679, 1757, 1875, 2694, 2741, 2763
NlaIV	GGNNCC	9 cut(s)	17, 154, 365, 978, 1012, 1050, 1740, 2351, 2585
NmeAIII	GCCGAG	4 cut(s)	692, 1022, 1982, 2581
NmuCI	GTSAC	1 cut(s)	2281
NsbI	TGCGCA	3 cut(s)	556, 1214, 2316
NsiI	ATGCAT	1 cut(s)	1983
NspI	RCATGY	2 cut(s)	1875, 2741
OliI	CACNNNNGTG	1 cut(s)	2409
PaeI	GCATGC	1 cut(s)	1875
PaqCI	CACCTGC	1 cut(s)	1981
PauI	GCGCGC	1 cut(s)	924
PceI	AGGCCT	2 cut(s)	428, 620
PciI	ACATGT	1 cut(s)	2737
PcsI	WCGNNNNNNNCGW	2 cut(s)	174, 664
PctI	GAATGC	2 cut(s)	483, 1361
PdiI	GCCGGC	1 cut(s)	1163
PdmI	GAANNNNTTC	1 cut(s)	678
PfeI	GAWTC	4 cut(s)	239, 1712, 2132, 2181
PflFI	GACNNNGTC	3 cut(s)	197, 361, 641
PflMI	CCANNNNNTGG	2 cut(s)	1295, 2029
PfoI	TCCNGGA	1 cut(s)	630
Ple19I	CGATCG	1 cut(s)	323
PleI	GAGTC	6 cut(s)	346, 352, 361, 1037, 1384, 1621
PpsI	GAGTC	6 cut(s)	346, 352, 361, 1037, 1384, 1621
Ppu21I	YACGTR	1 cut(s)	1184
PpuMI	RGGWCCY	1 cut(s)	976
PscI	ACATGT	1 cut(s)	2737
PshBI	ATTAAT	1 cut(s)	2256
Psp5II	RGGWCCY	1 cut(s)	976
Psp6I	CCWGG	5 cut(s)	423, 620, 630, 1798, 1833
PspFI	CCCAGC	3 cut(s)	1385, 1485, 2210
PspGI	CCWGG	5 cut(s)	423, 620, 630, 1798, 1833
PspN4I	GGNNCC	9 cut(s)	17, 154, 365, 978, 1012, 1050, 1740, 2351, 2585
PspOMI	GGGCCC	1 cut(s)	152
PspPI	GGNCC	8 cut(s)	152, 153, 363, 535, 976, 1144, 1796, 2553
PspPPI	RGGWCCY	1 cut(s)	976
PsrI	GAACNNNNNNTAC	2 cut(s)	1731, 1763
PstI	CTGCAG	3 cut(s)	531, 1222, 2524
PstNI	CAGNNNCTG	2 cut(s)	854, 2029
PsuI	RGATCY	2 cut(s)	2074, 2386
PsyI	GACNNNGTC	3 cut(s)	197, 361, 641
PteI	GCGCGC	1 cut(s)	924
PvuI	CGATCG	1 cut(s)	323
PvuII	CAGCTG	1 cut(s)	526
RigI	GGCCGGCC	1 cut(s)	1165
RsaI	GTAC	8 cut(s)	370, 722, 887, 986, 1182, 1276, 1724, 1806
RsaNI	GTAC	8 cut(s)	369, 721, 886, 985, 1181, 1275, 1723, 1805
RseI	CAYNNNNRTG	3 cut(s)	1596, 2225, 2409
SacII	CCGCGG	1 cut(s)	96
SalI	GTCGAC	1 cut(s)	355
SaqAI	TTAA	8 cut(s)	725, 1448, 1500, 1587, 1653, 1845, 2150, 2256
Sau3AI	GATC	9 cut(s)	100, 320, 474, 1570, 1921, 2008, 2074, 2386, 2476
Sau96I	GGNCC	8 cut(s)	152, 153, 363, 535, 976, 1144, 1796, 2553
ScaI	AGTACT	1 cut(s)	722
SchI	GAGTC	6 cut(s)	347, 352, 362, 1037, 1385, 1622
ScrFI	CCNGG	7 cut(s)	13, 425, 622, 632, 776, 1800, 1835
SduI	GDGCHC	3 cut(s)	20, 156, 605
SfcI	CTRYAG	5 cut(s)	527, 1218, 1791, 1885, 2520
Sfr303I	CCGCGG	1 cut(s)	96
SgrBI	CCGCGG	1 cut(s)	96
SinI	GGWCC	6 cut(s)	363, 535, 976, 1144, 1796, 2553
SmiMI	CAYNNNNRTG	3 cut(s)	1596, 2225, 2409
SmlI	CTYRAG	2 cut(s)	1934, 2344
SmoI	CTYRAG	2 cut(s)	1934, 2344
SphI	GCATGC	1 cut(s)	1875
Sse9I	AATT	9 cut(s)	380, 859, 880, 916, 1033, 1464, 1496, 1772, 2265
SseBI	AGGCCT	2 cut(s)	428, 620
SspI	AATATT	1 cut(s)	1704
SspMI	CTAG	5 cut(s)	197, 1122, 1604, 2108, 2381
StuI	AGGCCT	2 cut(s)	428, 620
StyD4I	CCNGG	7 cut(s)	11, 423, 620, 630, 774, 1798, 1833
StyI	CCWWGG	4 cut(s)	729, 801, 1360, 2690
TaaI	ACNGT	4 cut(s)	37, 850, 1886, 2194
TaiI	ACGT	3 cut(s)	207, 1186, 1323
TaqI	TCGA	5 cut(s)	39, 138, 237, 356, 1265
TaqII	GACCGA	2 cut(s)	351, 2489
TasI	AATT	9 cut(s)	380, 859, 880, 916, 1033, 1464, 1496, 1772, 2265
TatI	WGTACW	3 cut(s)	720, 1722, 1804
TauI	GCSGC	4 cut(s)	95, 594, 653, 2715
TfiI	GAWTC	4 cut(s)	239, 1712, 2132, 2181
Tru1I	TTAA	8 cut(s)	725, 1448, 1500, 1587, 1653, 1845, 2150, 2256
Tru9I	TTAA	8 cut(s)	725, 1448, 1500, 1587, 1653, 1845, 2150, 2256
TscAI	CASTG	5 cut(s)	76, 484, 1059, 1222, 1583
TseFI	GTSAC	1 cut(s)	2281
TseI	GCWGC	8 cut(s)	30, 47, 60, 526, 529, 808, 1526, 1672
Tsp45I	GTSAC	1 cut(s)	2281
TspDTI	ATGAA	4 cut(s)	376, 1368, 1742, 2720
TspGWI	ACGGA	3 cut(s)	348, 777, 2421
TspRI	CASTG	5 cut(s)	76, 484, 1059, 1222, 1583
Tth111I	GACNNNGTC	3 cut(s)	197, 361, 641
Van91I	CCANNNNNTGG	2 cut(s)	1295, 2029
VpaK11BI	GGWCC	6 cut(s)	363, 535, 976, 1144, 1796, 2553
VspI	ATTAAT	1 cut(s)	2256
XapI	RAATTY	5 cut(s)	859, 880, 1033, 1464, 1772
XceI	RCATGY	2 cut(s)	1875, 2741
XcmI	CCANNNNNNNNNTGG	2 cut(s)	580, 2030
XmiI	GTMKAC	1 cut(s)	356
XmnI	GAANNNNTTC	1 cut(s)	678
XspI	CTAG	5 cut(s)	197, 1122, 1604, 2108, 2381
ZrmI	AGTACT	1 cut(s)	722
Zsp2I	ATGCAT	1 cut(s)	1983

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.