pycom111g00950

Peptidase family M48

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0006709
Physical Location & Seq
Forward (+)
610543 .. 612177
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGGCTTCCGCTGCCGCGCCCTTGATCTCTTCTCTCTGCCTAACACAGAACCTTAGTTCTCTTAGTTTTAGCTCCGTGGCTTCGGATCATTTGCGGTTCGGTTCGCCCACCGTCGAATTCGCCGCCGTCAAGAAGTACCGGAGAGTTGGAATTCGAGTTCCTGTGTGCAGAGCTGCCTCCGTTGTGTTTAGCAACCTCGATGCCGATGATTTTCGGCACCCTTTGGATAAACAGAACACGCTGATTTTGAGAGCGATTCCGGGATTGAATGAGTTTGGAAAGATTCTGCTAGGATCTGTTGCAGAGCAAGTCATGCTTCTTGAGAACATCGGAACGTCCCTTCTTGTTTCGAAAGATCAGCTTTCTGGCCTACATCAATTGATGGTGGAGGCTGCGGAGATTCTCAGCATAGATGCTCCTGATTTATATGTTCGTCAAAGTCCTGTACCAAATGCGTATACTTTAGCAATAAGTGGTAAAAAGCCGTTTGTTGTTGTTCATACTAGTCTCGTGGAGCTTTTGACACAAGAAGAGCTGCAGGCTGTTTTGGCTCATGAGTTGGGTCATTTGAAATGCGACCATGGTGTGTGGCTTACGTTTGCAAATATTCTTACCCTTGGAGCTTATACAATACCTGGGCTAGGTGGGATGATTGCTCAGAGGTTGGAAGAACAGTTATTTCGTTGGCTTCGAGCAGCAGAGCTAACTTGTGATCGTGCAGCTCTTCTTGTTGCACAAAACCCAAAGAGGTGGTCGTCTCTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M48

Protein Analysis

256

Amino Acids

27.83

Weight (kDa)

6.65

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M48 PF01435 124 - 207 1.3e-14 Peptidase family M48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 216
AccI GTMKAC 1 cut(s) 458
AccII CGCG 1 cut(s) 17
AciI CCGC 5 cut(s) 9, 15, 94, 123, 395
AclWI GGATC 2 cut(s) 93, 301
AcsI RAATTY 2 cut(s) 116, 150
AfaI GTAC 2 cut(s) 137, 447
AfiI CCNNNNNNNGG 1 cut(s) 641
AgsI TTSAA 2 cut(s) 268, 571
AhlI ACTAGT 1 cut(s) 503
AjnI CCWGG 1 cut(s) 634
AloI GAACNNNNNNTCC 2 cut(s) 141, 173
AluBI AGCT 8 cut(s) 72, 173, 361, 517, 535, 623, 703, 722
AluI AGCT 8 cut(s) 72, 173, 361, 517, 535, 623, 703, 722
Alw26I GTCTC 2 cut(s) 512, 762
AlwI GGATC 2 cut(s) 93, 301
AoxI GGCC 1 cut(s) 367
ApeKI GCWGC 6 cut(s) 11, 173, 392, 535, 695, 719
ApoI RAATTY 2 cut(s) 116, 150
ArsI GACNNNNNNTTYG 1 cut(s) 737
AspLEI GCGC 1 cut(s) 19
AsuC2I CCSGG 1 cut(s) 261
AsuII TTCGAA 1 cut(s) 350
BanI GGYRCC 1 cut(s) 216
BauI CACGAG 1 cut(s) 509
BbvI GCAGC 5 cut(s) 160, 379, 522, 707, 731
BccI CCATC 1 cut(s) 376
BceAI ACGGC 2 cut(s) 110, 469
BciT130I CCWGG 1 cut(s) 636
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 2 cut(s) 512, 762
BcuI ACTAGT 1 cut(s) 503
BfaI CTAG 3 cut(s) 290, 504, 641
BfmI CTRYAG 1 cut(s) 536
BisI GCNGC 8 cut(s) 12, 15, 123, 174, 393, 536, 696, 720
BlsI GCNGC 8 cut(s) 13, 16, 124, 175, 394, 537, 697, 721
Bme1390I CCNGG 2 cut(s) 261, 636
BmiI GGNNCC 1 cut(s) 218
BmrFI CCNGG 2 cut(s) 261, 636
BmsI GCATC 2 cut(s) 190, 403
Bpu14I TTCGAA 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 261
BsaJI CCNNGG 4 cut(s) 75, 580, 616, 635
BsaWI WCCGGW 1 cut(s) 138
Bsc4I CCNNNNNNNGG 1 cut(s) 641
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 4 cut(s) 75, 580, 616, 635
BseGI GGATG 1 cut(s) 654
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMII CTCAG 2 cut(s) 418, 671
BseXI GCAGC 5 cut(s) 160, 379, 522, 707, 731
BsgI GTGCAG 2 cut(s) 187, 738
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 369
BshNI GGYRCC 1 cut(s) 216
BsiSI CCGG 2 cut(s) 139, 260
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 2 cut(s) 512, 762
BsmBI CGTCTC 1 cut(s) 762
BsmFI GGGAC 1 cut(s) 322
BsnI GGCC 1 cut(s) 369
Bsp119I TTCGAA 1 cut(s) 350
Bsp143I GATC 5 cut(s) 24, 85, 293, 355, 712
Bsp19I CCATGG 1 cut(s) 580
BspACI CCGC 5 cut(s) 9, 15, 94, 123, 395
BspANI GGCC 1 cut(s) 369
BspCNI CTCAG 2 cut(s) 417, 670
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 1 cut(s) 218
BspMAI CTGCAG 1 cut(s) 540
BspPI GGATC 2 cut(s) 93, 301
BspQI GCTCTTC 2 cut(s) 525, 729
BspT104I TTCGAA 1 cut(s) 350
BspT107I GGYRCC 1 cut(s) 216
BssECI CCNNGG 4 cut(s) 75, 580, 616, 635
BssMI GATC 5 cut(s) 24, 85, 293, 355, 712
BssNAI GTATAC 1 cut(s) 459
BssSI CACGAG 1 cut(s) 509
BssT1I CCWWGG 2 cut(s) 580, 616
Bst1107I GTATAC 1 cut(s) 459
Bst2BI CACGAG 1 cut(s) 509
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 2 cut(s) 112, 675
Bst6I CTCTTC 3 cut(s) 34, 525, 729
BstAPI GCANNNNNTGC 1 cut(s) 313
BstBI TTCGAA 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 4 cut(s) 53, 62, 404, 657
BstDSI CCRYGG 2 cut(s) 75, 580
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 1 cut(s) 654
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 5 cut(s) 27, 88, 296, 358, 715
BstMAI GTCTC 2 cut(s) 512, 762
BstMBI GATC 5 cut(s) 24, 85, 293, 355, 712
BstMWI GCNNNNNNNGC 3 cut(s) 11, 313, 548
BstNI CCWGG 1 cut(s) 636
BstSCI CCNGG 2 cut(s) 259, 634
BstSFI CTRYAG 1 cut(s) 536
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 5 cut(s) 160, 379, 522, 707, 731
BstX2I RGATCY 1 cut(s) 293
BstXI CCANNNNNNTGG 1 cut(s) 750
BstYI RGATCY 1 cut(s) 293
BstZ17I GTATAC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 369
BtgI CCRYGG 2 cut(s) 75, 580
BtsCI GGATG 1 cut(s) 654
Cac8I GCNNGC 1 cut(s) 540
CciI TCATGA 1 cut(s) 553
CfoI GCGC 1 cut(s) 19
Csp6I GTAC 2 cut(s) 136, 446
CviAII CATG 3 cut(s) 313, 554, 581
CviQI GTAC 2 cut(s) 136, 446
DdeI CTNAG 4 cut(s) 53, 62, 404, 657
DpnI GATC 5 cut(s) 26, 87, 295, 357, 714
DpnII GATC 5 cut(s) 24, 85, 293, 355, 712
Eam1104I CTCTTC 3 cut(s) 34, 525, 729
EarI CTCTTC 3 cut(s) 34, 525, 729
Eco130I CCWWGG 2 cut(s) 580, 616
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoRI GAATTC 2 cut(s) 116, 150
EcoRII CCWGG 1 cut(s) 634
EcoT14I CCWWGG 2 cut(s) 580, 616
ErhI CCWWGG 2 cut(s) 580, 616
Esp3I CGTCTC 1 cut(s) 762
FaeI CATG 3 cut(s) 316, 557, 584
FaiI YATR 9 cut(s) 314, 410, 427, 429, 459, 501, 555, 582, 627
FalI AAGNNNNNCTT 4 cut(s) 300, 332, 345, 377
FaqI GGGAC 1 cut(s) 322
FatI CATG 3 cut(s) 312, 553, 580
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 8 cut(s) 12, 15, 123, 174, 393, 536, 696, 720
FokI GGATG 1 cut(s) 661
Fsp4HI GCNGC 8 cut(s) 12, 15, 123, 174, 393, 536, 696, 720
FspBI CTAG 3 cut(s) 290, 504, 641
GlaI GCGC 1 cut(s) 18
GluI GCNGC 8 cut(s) 12, 15, 123, 174, 393, 536, 696, 720
HaeIII GGCC 1 cut(s) 369
HapII CCGG 2 cut(s) 139, 260
HhaI GCGC 1 cut(s) 19
Hin1II CATG 3 cut(s) 316, 557, 584
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 3 cut(s) 256, 283, 400
HpaII CCGG 2 cut(s) 139, 260
Hpy166II GTNNAC 1 cut(s) 459
Hpy188I TCNGA 3 cut(s) 85, 332, 660
Hpy188III TCNNGA 4 cut(s) 130, 320, 419, 554
Hpy8I GTNNAC 1 cut(s) 459
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 2 cut(s) 112, 675
HpyCH4IV ACGT 2 cut(s) 335, 596
HpyCH4V TGCA 6 cut(s) 168, 302, 538, 602, 719, 734
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 313, 548
HpyF3I CTNAG 4 cut(s) 53, 62, 404, 657
HpySE526I ACGT 2 cut(s) 335, 596
Hsp92II CATG 3 cut(s) 316, 557, 584
HspAI GCGC 1 cut(s) 17
Kzo9I GATC 5 cut(s) 24, 85, 293, 355, 712
LguI GCTCTTC 2 cut(s) 525, 729
LmnI GCTCC 4 cut(s) 77, 421, 514, 620
LpnPI CCDG 9 cut(s) 152, 174, 273, 351, 432, 456, 524, 621, 648
Lsp1109I GCAGC 5 cut(s) 160, 379, 522, 707, 731
LweI GCATC 2 cut(s) 190, 403
MaeI CTAG 3 cut(s) 290, 504, 641
MaeII ACGT 2 cut(s) 335, 596
MalI GATC 5 cut(s) 26, 87, 295, 357, 714
MboI GATC 5 cut(s) 24, 85, 293, 355, 712
MboII GAAGA 4 cut(s) 21, 542, 680, 716
MfeI CAATTG 1 cut(s) 377
MflI RGATCY 1 cut(s) 293
MluCI AATT 3 cut(s) 116, 150, 377
MmeI TCCRAC 2 cut(s) 127, 645
MnlI CCTC 5 cut(s) 187, 206, 382, 654, 741
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 2 cut(s) 139, 260
MspR9I CCNGG 2 cut(s) 261, 636
MunI CAATTG 1 cut(s) 377
MvaI CCWGG 1 cut(s) 636
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 11, 313, 548
NciI CCSGG 1 cut(s) 261
NcoI CCATGG 1 cut(s) 580
NdeII GATC 5 cut(s) 24, 85, 293, 355, 712
NlaIII CATG 3 cut(s) 316, 557, 584
NlaIV GGNNCC 1 cut(s) 218
NspV TTCGAA 1 cut(s) 350
PagI TCATGA 1 cut(s) 553
PciSI GCTCTTC 2 cut(s) 525, 729
PcsI WCGNNNNNNNCGW 1 cut(s) 688
PfeI GAWTC 3 cut(s) 256, 283, 400
PfoI TCCNGGA 1 cut(s) 259
PkrI GCNGC 8 cut(s) 13, 16, 124, 175, 394, 537, 697, 721
Psp6I CCWGG 1 cut(s) 634
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 1 cut(s) 218
PstI CTGCAG 1 cut(s) 540
PsuI RGATCY 1 cut(s) 293
RsaI GTAC 2 cut(s) 137, 447
RsaNI GTAC 2 cut(s) 136, 446
SapI GCTCTTC 2 cut(s) 525, 729
SatI GCNGC 8 cut(s) 12, 15, 123, 174, 393, 536, 696, 720
Sau3AI GATC 5 cut(s) 24, 85, 293, 355, 712
ScrFI CCNGG 2 cut(s) 261, 636
SfaNI GCATC 2 cut(s) 190, 403
SfcI CTRYAG 1 cut(s) 536
SfuI TTCGAA 1 cut(s) 350
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SpeI ACTAGT 1 cut(s) 503
Sse9I AATT 3 cut(s) 116, 150, 377
SsiI CCGC 5 cut(s) 9, 15, 94, 123, 395
SspI AATATT 1 cut(s) 607
SspMI CTAG 3 cut(s) 290, 504, 641
StyD4I CCNGG 2 cut(s) 259, 634
StyI CCWWGG 2 cut(s) 580, 616
TaaI ACNGT 2 cut(s) 112, 675
TaiI ACGT 2 cut(s) 338, 599
TaqI TCGA 5 cut(s) 114, 154, 198, 350, 691
TasI AATT 3 cut(s) 116, 150, 377
TauI GCSGC 2 cut(s) 17, 125
TfiI GAWTC 3 cut(s) 256, 283, 400
TseI GCWGC 6 cut(s) 11, 173, 392, 535, 695, 719
TspDTI ATGAA 1 cut(s) 488
TspGWI ACGGA 2 cut(s) 64, 169
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 2 cut(s) 116, 150
XmiI GTMKAC 1 cut(s) 458
XspI CTAG 3 cut(s) 290, 504, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.