pycom111g03620

Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH ubiquinone oxidoreductase complex (complex I)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0005361
Physical Location & Seq
Reverse (-)
2224964 .. 2225796
833 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
ATGATTGATGTTGCTGAAGATTCAACGTTTCGTTTTGTTCCGTCTTCACAGCCTACACCAGCGACTCTTTATCTTGCAAAACGCTGCAAATGGGCTGGAAATGAAGAAATAGCAAAGCTTCAACATATCGAGGTTTGCCCCAAAGCAATGGAGTCGACTCAAACTATGGCTGAAAGAATTGCTTCCGACGGAGGTGGAGCTCTTATAATTGACTATGGCCTCAATGGAGTAGTATCAGATAGTCTGCAGGTCTGTTGCTTTTACTTTTGCAGTACTTCAATTTGTTGCAGGCCATTCGGAAACATAAATTTGTCAACATTCTGGATGATCCTGGCTCTGCTCGATCTTAGTGCATATGTTGATTTTGCTTCCATCAGGCACTCCGCTGAGGAAATTTCAGGAGAGATTTCTGTCCATGGCCCGATTACTCAGTCTCAGTTCCTTGGTTCTCTTGGGATAAATTTCCGAGCGGAAGCATTAACACAAAACTGTACAGAAGAACAATTCGAGTCTCTAAGGAACGGATACTGGCAACTGGTTGGCGAAGGTGAGGCCCCCTTCTGGGAGGGGGCTGATGAAAAAGCGCCCATCGGAATGGGCACTCGGTATTTGGCAATGGCCATTGTGAACAAGAAACAAGGAGTTCCTGTTCCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
Methyltransf_28

Protein Analysis

220

Amino Acids

24.01

Weight (kDa)

4.74

Isoelectric Point (pI)

50.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_28 PF02636 6 - 141 1.1e-09 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 238
AccBSI CCGCTC 1 cut(s) 470
AccI GTMKAC 1 cut(s) 155
AciI CCGC 2 cut(s) 384, 470
AclI AACGTT 1 cut(s) 26
AclWI GGATC 1 cut(s) 322
AcoI YGGCCR 1 cut(s) 618
AcsI RAATTY 3 cut(s) 307, 393, 460
AcuI CTGAAG 1 cut(s) 36
AfaI GTAC 2 cut(s) 274, 493
AfiI CCNNNNNNNGG 2 cut(s) 561, 562
AgsI TTSAA 3 cut(s) 24, 122, 279
AjnI CCWGG 1 cut(s) 330
AloI GAACNNNNNNTCC 1 cut(s) 633
AluBI AGCT 2 cut(s) 118, 200
AluI AGCT 2 cut(s) 118, 200
Alw21I GWGCWC 1 cut(s) 202
Alw26I GTCTC 2 cut(s) 438, 516
AlwI GGATC 1 cut(s) 322
AoxI GGCC 5 cut(s) 217, 290, 418, 552, 618
ApeKI GCWGC 1 cut(s) 84
ApoI RAATTY 3 cut(s) 307, 393, 460
Asp700I GAANNNNTTC 1 cut(s) 181
AspLEI GCGC 1 cut(s) 586
AspS9I GGNCC 2 cut(s) 419, 553
AsuHPI GGTGA 1 cut(s) 560
BaeGI GKGCMC 1 cut(s) 602
BalI TGGCCA 1 cut(s) 620
BanII GRGCYC 1 cut(s) 202
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 202
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 71
BccI CCATC 2 cut(s) 380, 596
BcgI CGANNNNNNTGC 6 cut(s) 135, 169, 277, 311, 332, 366
BciT130I CCWGG 1 cut(s) 332
BciVI GTATCC 1 cut(s) 518
BcoDI GTCTC 2 cut(s) 438, 516
BfmI CTRYAG 1 cut(s) 245
BfoI RGCGCY 1 cut(s) 587
BfuAI ACCTGC 1 cut(s) 238
BfuI GTATCC 1 cut(s) 518
BisI GCNGC 1 cut(s) 85
BlsI GCNGC 1 cut(s) 86
BmcAI AGTACT 1 cut(s) 274
Bme1390I CCNGG 1 cut(s) 332
BmgT120I GGNCC 2 cut(s) 419, 553
BmiI GGNNCC 1 cut(s) 555
BmrFI CCNGG 1 cut(s) 332
BpiI GAAGAC 1 cut(s) 36
Bpu10I CCTNAGC 1 cut(s) 387
BsaJI CCNNGG 2 cut(s) 415, 442
BsaXI ACNNNNNCTCC 1 cut(s) 633
Bsc4I CCNNNNNNNGG 2 cut(s) 561, 562
Bse1I ACTGG 2 cut(s) 533, 540
Bse3DI GCAATG 2 cut(s) 153, 621
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 2 cut(s) 415, 442
BseGI GGATG 1 cut(s) 330
BseLI CCNNNNNNNGG 2 cut(s) 561, 562
BseMI GCAATG 2 cut(s) 153, 621
BseMII CTCAG 3 cut(s) 378, 443, 449
BseNI ACTGG 2 cut(s) 533, 540
BseSI GKGCMC 1 cut(s) 602
BseXI GCAGC 1 cut(s) 71
BshFI GGCC 5 cut(s) 219, 292, 420, 554, 620
BsiHKAI GWGCWC 1 cut(s) 202
BslI CCNNNNNNNGG 2 cut(s) 561, 562
BsmAI GTCTC 2 cut(s) 438, 516
BsnI GGCC 5 cut(s) 219, 292, 420, 554, 620
Bsp1286I GDGCHC 2 cut(s) 202, 602
Bsp1407I TGTACA 1 cut(s) 491
Bsp143I GATC 2 cut(s) 327, 343
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 2 cut(s) 384, 470
BspANI GGCC 5 cut(s) 219, 292, 420, 554, 620
BspCNI CTCAG 3 cut(s) 379, 442, 448
BspLI GGNNCC 1 cut(s) 555
BspMAI CTGCAG 1 cut(s) 249
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 1 cut(s) 322
BsrBI CCGCTC 1 cut(s) 470
BsrDI GCAATG 2 cut(s) 153, 621
BsrGI TGTACA 1 cut(s) 491
BsrI ACTGG 2 cut(s) 533, 540
BssECI CCNNGG 2 cut(s) 415, 442
BssMI GATC 2 cut(s) 327, 343
BssT1I CCWWGG 2 cut(s) 415, 442
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 1 cut(s) 491
BstAUI TGTACA 1 cut(s) 491
BstC8I GCNNGC 1 cut(s) 290
BstDEI CTNAG 5 cut(s) 347, 387, 429, 435, 515
BstDSI CCRYGG 1 cut(s) 415
BstF5I GGATG 1 cut(s) 330
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 1 cut(s) 586
BstKTI GATC 2 cut(s) 330, 346
BstMAI GTCTC 2 cut(s) 438, 516
BstMBI GATC 2 cut(s) 327, 343
BstNI CCWGG 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 330
BstSFI CTRYAG 1 cut(s) 245
BstSLI GKGCMC 1 cut(s) 602
BstV1I GCAGC 1 cut(s) 71
BstV2I GAAGAC 1 cut(s) 36
BstXI CCANNNNNNTGG 2 cut(s) 148, 595
BsuI GTATCC 1 cut(s) 518
BsuRI GGCC 5 cut(s) 219, 292, 420, 554, 620
BtgI CCRYGG 1 cut(s) 415
BtsCI GGATG 1 cut(s) 330
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 290
CfoI GCGC 1 cut(s) 586
Cfr13I GGNCC 2 cut(s) 419, 553
Csp6I GTAC 2 cut(s) 273, 492
CviAII CATG 1 cut(s) 416
CviQI GTAC 2 cut(s) 273, 492
DdeI CTNAG 5 cut(s) 347, 387, 429, 435, 515
DpnI GATC 2 cut(s) 329, 345
DpnII GATC 2 cut(s) 327, 343
EaeI YGGCCR 1 cut(s) 618
Ecl136II GAGCTC 1 cut(s) 200
Eco130I CCWWGG 2 cut(s) 415, 442
Eco24I GRGCYC 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 36
EcoICRI GAGCTC 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 330
EcoT14I CCWWGG 2 cut(s) 415, 442
EcoT38I GRGCYC 1 cut(s) 202
ErhI CCWWGG 2 cut(s) 415, 442
FaeI CATG 1 cut(s) 419
FaiI YATR 8 cut(s) 126, 167, 206, 216, 305, 355, 357, 417
FalI AAGNNNNNCTT 2 cut(s) 166, 198
FatI CATG 1 cut(s) 415
FauNDI CATATG 1 cut(s) 355
FblI GTMKAC 1 cut(s) 155
Fnu4HI GCNGC 1 cut(s) 85
FokI GGATG 1 cut(s) 337
FriOI GRGCYC 1 cut(s) 202
Fsp4HI GCNGC 1 cut(s) 85
GlaI GCGC 1 cut(s) 585
GluI GCNGC 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 5 cut(s) 219, 292, 420, 554, 620
HhaI GCGC 1 cut(s) 586
Hin1II CATG 1 cut(s) 419
Hin6I GCGC 1 cut(s) 584
HinP1I GCGC 1 cut(s) 584
HincII GTYRAC 2 cut(s) 156, 315
HindII GTYRAC 2 cut(s) 156, 315
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 5 cut(s) 20, 64, 152, 157, 509
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 3 cut(s) 156, 315, 628
Hpy188I TCNGA 5 cut(s) 187, 238, 299, 467, 593
Hpy188III TCNNGA 2 cut(s) 322, 399
Hpy8I GTNNAC 3 cut(s) 156, 315, 628
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 539, 568
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 6 cut(s) 77, 87, 247, 270, 288, 353
HpyF3I CTNAG 5 cut(s) 347, 387, 429, 435, 515
HpySE526I ACGT 1 cut(s) 26
Hsp92II CATG 1 cut(s) 419
HspAI GCGC 1 cut(s) 584
Kzo9I GATC 2 cut(s) 327, 343
LmnI GCTCC 1 cut(s) 197
Lsp1109I GCAGC 1 cut(s) 71
MaeII ACGT 1 cut(s) 26
MalI GATC 2 cut(s) 329, 345
MbiI CCGCTC 1 cut(s) 470
MboI GATC 2 cut(s) 327, 343
MboII GAAGA 4 cut(s) 29, 36, 116, 509
MhlI GDGCHC 2 cut(s) 202, 602
MlsI TGGCCA 1 cut(s) 620
MluCI AATT 7 cut(s) 177, 207, 279, 307, 393, 460, 503
MluNI TGGCCA 1 cut(s) 620
MlyI GAGTC 4 cut(s) 58, 151, 161, 518
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 6 cut(s) 124, 185, 230, 382, 544, 559
Mox20I TGGCCA 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 181
MscI TGGCCA 1 cut(s) 620
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 1 cut(s) 593
Msp20I TGGCCA 1 cut(s) 620
MspA1I CMGCKG 1 cut(s) 386
MspR9I CCNGG 1 cut(s) 332
MvaI CCWGG 1 cut(s) 332
NcoI CCATGG 1 cut(s) 415
NdeI CATATG 1 cut(s) 355
NdeII GATC 2 cut(s) 327, 343
NlaIII CATG 1 cut(s) 419
NlaIV GGNNCC 1 cut(s) 555
PdmI GAANNNNTTC 1 cut(s) 181
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 1 cut(s) 86
PleI GAGTC 4 cut(s) 58, 151, 160, 517
PpsI GAGTC 4 cut(s) 58, 151, 160, 517
PsiI TTATAA 1 cut(s) 206
Psp124BI GAGCTC 1 cut(s) 202
Psp1406I AACGTT 1 cut(s) 26
Psp6I CCWGG 1 cut(s) 330
PspGI CCWGG 1 cut(s) 330
PspN4I GGNNCC 1 cut(s) 555
PspPI GGNCC 2 cut(s) 419, 553
PstI CTGCAG 1 cut(s) 249
RsaI GTAC 2 cut(s) 274, 493
RsaNI GTAC 2 cut(s) 273, 492
RseI CAYNNNNRTG 1 cut(s) 593
SacI GAGCTC 1 cut(s) 202
SalI GTCGAC 1 cut(s) 154
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 1 cut(s) 85
Sau3AI GATC 2 cut(s) 327, 343
Sau96I GGNCC 2 cut(s) 419, 553
ScaI AGTACT 1 cut(s) 274
SchI GAGTC 4 cut(s) 58, 151, 161, 518
ScrFI CCNGG 1 cut(s) 332
SduI GDGCHC 2 cut(s) 202, 602
SetI ASST 7 cut(s) 29, 120, 135, 196, 202, 252, 550
SfcI CTRYAG 1 cut(s) 245
SmiMI CAYNNNNRTG 1 cut(s) 593
Sse9I AATT 7 cut(s) 177, 207, 279, 307, 393, 460, 503
SsiI CCGC 2 cut(s) 384, 470
SstI GAGCTC 1 cut(s) 202
StyD4I CCNGG 1 cut(s) 330
StyI CCWWGG 2 cut(s) 415, 442
TaaI ACNGT 1 cut(s) 491
TaiI ACGT 1 cut(s) 29
TaqI TCGA 4 cut(s) 129, 155, 342, 507
TasI AATT 7 cut(s) 177, 207, 279, 307, 393, 460, 503
TatI WGTACW 2 cut(s) 272, 491
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TseI GCWGC 1 cut(s) 84
TspDTI ATGAA 2 cut(s) 117, 591
TspGWI ACGGA 3 cut(s) 30, 204, 537
XapI RAATTY 3 cut(s) 307, 393, 460
XmiI GTMKAC 1 cut(s) 155
XmnI GAANNNNTTC 1 cut(s) 181
ZrmI AGTACT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.