Rh4CG388500

Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH ubiquinone oxidoreductase complex (complex I)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005361
Physical Location & Seq
Forward (+)
63967733 .. 63969426
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG388500.1

Sequence Viewer

Length: 579 bp
ATGAGTTTTATGATGCTTTACCAGTTCATCAATTTCAGGTTTGTATGGTCTTTTCTAACATTTGTATTCAAATGGTCATTCTTTGCGTATCATCTTATTCTTAATCTCACTAATCTTTTTTTCCAGAGGGCTTCTCGTGGCTGGTGTGAGAAAATGGTGGATGTTGCAGAAGATTCAAAGTTCCGTTTTGTTCTTTCTTCACAGCCTACACCAGCAACGCTTTATCTGGCAAAACGCTGCAAATGGGCTGGAATGGAAGAAATAGCGAAGCTTGATCATGTTGAGGTTTGCCCTGCATCAATGGAGTTGACTCAAAGTATTGCTGATAGAATTGCTTCTGATGGGGGTGGTGCTCTTGTAATTGATTATGGTCAGAATGGAGTAGTCTCTGACAGTCTGCAGGCAATTCGGAAACATAAGTTTGTTGACATTCTAGACGATCCAGGATCAGCTGATCTCAGTGCCTACGTTGATTTTGCTTCCATCATGCATTCTGCTGAGGAAGCTTCAGGAGATGTGTCTGTTCATGGCCCTATTACTCAGTCTCAGTTCCTTGGCTCTCTTGGGATAAATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
Methyltransf_28

Protein Analysis

192

Amino Acids

21.52

Weight (kDa)

5.27

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_28 PF02636 23 - 178 2.4e-27 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 434, 454
AcsI RAATTY 1 cut(s) 571
AcuI CTGAAG 1 cut(s) 492
AgsI TTSAA 2 cut(s) 70, 177
AjnI CCWGG 1 cut(s) 442
AluBI AGCT 3 cut(s) 271, 452, 506
AluI AGCT 3 cut(s) 271, 452, 506
Alw21I GWGCWC 1 cut(s) 355
Alw26I GTCTC 2 cut(s) 391, 549
AlwI GGATC 2 cut(s) 434, 454
AoxI GGCC 1 cut(s) 529
ApeKI GCWGC 1 cut(s) 237
ApoI RAATTY 1 cut(s) 571
Asp700I GAANNNNTTC 1 cut(s) 334
AspS9I GGNCC 1 cut(s) 530
BauI CACGAG 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 355
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 1 cut(s) 224
BccI CCATC 2 cut(s) 335, 491
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 1 cut(s) 444
BclI TGATCA 1 cut(s) 274
BcoDI GTCTC 2 cut(s) 391, 549
BfaI CTAG 1 cut(s) 434
BfmI CTRYAG 1 cut(s) 398
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
Bme1390I CCNGG 1 cut(s) 444
BmgT120I GGNCC 1 cut(s) 530
BmrFI CCNGG 1 cut(s) 444
BmsI GCATC 2 cut(s) 3, 305
BplI GAGNNNNNCTC 2 cut(s) 118, 150
Bpu10I CCTNAGC 1 cut(s) 498
BsaJI CCNNGG 1 cut(s) 553
Bse1I ACTGG 1 cut(s) 22
BseBI CCWGG 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 553
BseGI GGATG 1 cut(s) 166
BseMII CTCAG 4 cut(s) 472, 489, 554, 560
BseNI ACTGG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 224
BshFI GGCC 1 cut(s) 531
BsiHKAI GWGCWC 1 cut(s) 355
BsmAI GTCTC 2 cut(s) 391, 549
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 1 cut(s) 531
Bsp1286I GDGCHC 1 cut(s) 355
Bsp143I GATC 4 cut(s) 274, 439, 446, 454
BspANI GGCC 1 cut(s) 531
BspCNI CTCAG 4 cut(s) 471, 490, 553, 559
BspMAI CTGCAG 1 cut(s) 402
BspPI GGATC 2 cut(s) 434, 454
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 553
BssMI GATC 4 cut(s) 274, 439, 446, 454
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 553
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 1 cut(s) 444
Bst4CI ACNGT 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 4 cut(s) 458, 498, 540, 546
BstF5I GGATG 1 cut(s) 166
BstKTI GATC 4 cut(s) 277, 442, 449, 457
BstMAI GTCTC 2 cut(s) 391, 549
BstMBI GATC 4 cut(s) 274, 439, 446, 454
BstMWI GCNNNNNNNGC 1 cut(s) 503
BstNI CCWGG 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 442
BstSFI CTRYAG 1 cut(s) 398
BstV1I GCAGC 1 cut(s) 224
BsuRI GGCC 1 cut(s) 531
BtsCI GGATG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 466
Cac8I GCNNGC 1 cut(s) 402
Cfr13I GGNCC 1 cut(s) 530
CviAII CATG 3 cut(s) 278, 487, 527
CviJI RGCY 9 cut(s) 131, 141, 205, 248, 271, 452, 506, 531, 558
CviKI_1 RGCY 9 cut(s) 131, 141, 205, 248, 271, 452, 506, 531, 558
DdeI CTNAG 4 cut(s) 458, 498, 540, 546
DpnI GATC 4 cut(s) 276, 441, 448, 456
DpnII GATC 4 cut(s) 274, 439, 446, 454
Eco130I CCWWGG 1 cut(s) 553
Eco57I CTGAAG 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 442
EcoT14I CCWWGG 1 cut(s) 553
EcoT22I ATGCAT 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 553
FaeI CATG 3 cut(s) 281, 490, 530
FaiI YATR 7 cut(s) 11, 46, 279, 369, 417, 488, 528
FatI CATG 3 cut(s) 277, 486, 526
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 1 cut(s) 238
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 1 cut(s) 238
FspBI CTAG 1 cut(s) 434
GluI GCNGC 1 cut(s) 238
HaeIII GGCC 1 cut(s) 531
Hin1II CATG 3 cut(s) 281, 490, 530
HincII GTYRAC 2 cut(s) 309, 427
HindII GTYRAC 2 cut(s) 309, 427
HindIII AAGCTT 2 cut(s) 269, 504
HinfI GANTC 2 cut(s) 173, 310
Hpy166II GTNNAC 2 cut(s) 309, 427
Hpy188I TCNGA 5 cut(s) 340, 375, 391, 411, 578
Hpy188III TCNNGA 3 cut(s) 124, 434, 510
Hpy8I GTNNAC 2 cut(s) 309, 427
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 5 cut(s) 167, 240, 296, 400, 490
HpyF10VI GCNNNNNNNGC 1 cut(s) 503
HpyF3I CTNAG 4 cut(s) 458, 498, 540, 546
HpySE526I ACGT 1 cut(s) 468
Hsp92II CATG 3 cut(s) 281, 490, 530
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 4 cut(s) 274, 439, 446, 454
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 2 cut(s) 3, 305
MaeI CTAG 1 cut(s) 434
MaeII ACGT 1 cut(s) 468
MalI GATC 4 cut(s) 276, 441, 448, 456
MboI GATC 4 cut(s) 274, 439, 446, 454
MboII GAAGA 3 cut(s) 182, 189, 269
MhlI GDGCHC 1 cut(s) 355
MluCI AATT 5 cut(s) 31, 330, 360, 405, 571
MlyI GAGTC 1 cut(s) 304
MnlI CCTC 3 cut(s) 120, 277, 493
Mph1103I ATGCAT 1 cut(s) 492
MroXI GAANNNNTTC 1 cut(s) 334
MseI TTAA 1 cut(s) 102
MspA1I CMGCKG 1 cut(s) 452
MspR9I CCNGG 1 cut(s) 444
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 1 cut(s) 444
MwoI GCNNNNNNNGC 1 cut(s) 503
NdeII GATC 4 cut(s) 274, 439, 446, 454
NlaIII CATG 3 cut(s) 281, 490, 530
NsiI ATGCAT 1 cut(s) 492
PctI GAATGC 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 334
PfeI GAWTC 1 cut(s) 173
PfoI TCCNGGA 1 cut(s) 442
PkrI GCNGC 1 cut(s) 239
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
Psp6I CCWGG 1 cut(s) 442
PspGI CCWGG 1 cut(s) 442
PspPI GGNCC 1 cut(s) 530
PstI CTGCAG 1 cut(s) 402
PvuII CAGCTG 1 cut(s) 452
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 4 cut(s) 274, 439, 446, 454
Sau96I GGNCC 1 cut(s) 530
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 1 cut(s) 444
SduI GDGCHC 1 cut(s) 355
SetI ASST 6 cut(s) 41, 273, 288, 454, 471, 508
SfaNI GCATC 2 cut(s) 3, 305
SfcI CTRYAG 1 cut(s) 398
Sse9I AATT 5 cut(s) 31, 330, 360, 405, 571
SspMI CTAG 1 cut(s) 434
StyD4I CCNGG 1 cut(s) 442
StyI CCWWGG 1 cut(s) 553
TaaI ACNGT 1 cut(s) 395
TaiI ACGT 1 cut(s) 471
TasI AATT 5 cut(s) 31, 330, 360, 405, 571
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 466
TseI GCWGC 1 cut(s) 237
TspDTI ATGAA 2 cut(s) 16, 515
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 1 cut(s) 466
XapI RAATTY 1 cut(s) 571
XbaI TCTAGA 1 cut(s) 433
XmnI GAANNNNTTC 1 cut(s) 334
XspI CTAG 1 cut(s) 434
Zsp2I ATGCAT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.