Rw2G050550

Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH ubiquinone oxidoreductase complex (complex I)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005361
Physical Location & Seq
Forward (+)
78132767 .. 78137396
4630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050550.1

Sequence Viewer

Length: 1428 bp
ATGTTGAGACGACTACTCCTTCAAGCTTCCACTACTCGTCGCCTTGCCGGTGCCCACTTCACTCCACCATCTTCACCTCCTTTGCTTCGTTATCGTCCACTTTCTTCTTCTTCTTCAACCCAAATCCCAGAGACTCATTTGGAGGACAACCCTGACCAACCCTCTGCCACCATCTCCATCGACCGCTCCGGCCTTTGCAATCCCCCCGAGCATTCCCATGAACCCTGTTCCGACTCCGAGCTCGTCAAGCACCTCAAAGGCATCATCAAGTTCCGCGGTGGCCCAATCTCGGTGGCTGAGTATATGGAGGAGGTTTTGACGAACCCGAAAGCTGGGTTCTACATGAACCGTGATGTGTTTGGAGCCGGAGGTGATTTTATCACCTCCCCAGAAGTGAGCCAGATGTTCGGGGAGATGGTTGGTGTTTGGGCGATGTCTCTGTGGGAGCAGATGGGGCAGCCAGGAAAAATGAACCTAGTAGAGCTAGGTCCAGGAAGAGGAACTCTCATGGCTGACCTTCTTCGGTATGAAATGGGTGCGTCCAAGTTTAAGAACTTCACTGAATCGTTGCATGTACACATGGTGGAGTGCAGTCCTGCATTACAGAAGCTCCAGCACCAGGCCCTGAAATGTGTGGATGAAGAGGGTACGGCTGAGAAAAGGACTGTCAGCACTTTGGCTAAAACTCCTGTGTCATGGCATGCCACATTCGACCAGGTTCCTTCAGGATTGCCATCTATTATCATCGCCCATGAGTTTTATGATGCTTTACCAGTTCATCAATTTCAGAGGGCTTCTTGTGGCTGGTGTGAGAAAATGGTGGATGTTGCTGAAGATTCAAAGTTCCGTTTTGTTCTTTCTTCACAGCCTACACCAGCAACTCTTTATCTGGCAAAACGCTGCAAATGGGCTGGAATGGAAGAAATAGCGAAGCTTGATCATGTTGAGGTTTGCCCTGCATCAACGGAGTTGACTCAAAGTATTGCTGATAGAATTGCTTCTGATGGGGGTGGTGCTCTTGTAATTGATTATGGTCAGAATGGAGCAGTCTCTGACAGTCTGCAGGCAATTCGGAAACATAAATTTGTTGACATTCTAGACGATCCAGGATCTGCTGATCTCAGTGCCTACGTTGATTTTGCTTCCATCAGGCATTCTGCTGAGGAAGCTTCAGGAGATGTGTCTGTTCATGGCCCTATTACTCAGTCTCAGTTCCTTGGCTCTCTTGGGATAAATTTCCGAGTGGAAGCATTATTACAGAACTGCACGGAGGAACAATTTGAATCTCTCAGGTCAGGGTATTGGCAACTGGTTGGAGAAGGTGAGGCTCCCTTCTGGGAGGGTCCTGACGAAAAAGTTCCCATCGGAATGGGTACCCGTTATTTGAAGTTGAAACAAGTGTTTGTTTCATGGTTTTCCAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
Methyltransf_28

Protein Analysis

475

Amino Acids

52.32

Weight (kDa)

5.43

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_28 PF02636 139 - 399 5.3e-63 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1373
AccB1I GGYRCC 2 cut(s) 50, 1373
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 1 cut(s) 276
AciI CCGC 3 cut(s) 184, 274, 276
AclWI GGATC 2 cut(s) 1097, 1117
AcsI RAATTY 2 cut(s) 1082, 1234
AcuI CTGAAG 3 cut(s) 708, 852, 1155
AdeI CACNNNGTG 1 cut(s) 583
AfaI GTAC 3 cut(s) 576, 649, 1375
AfiI CCNNNNNNNGG 4 cut(s) 289, 332, 497, 619
AgsI TTSAA 6 cut(s) 23, 117, 840, 1283, 1387, 1393
AjnI CCWGG 5 cut(s) 460, 490, 618, 714, 1105
AluBI AGCT 7 cut(s) 26, 241, 332, 484, 610, 934, 1169
AluI AGCT 7 cut(s) 26, 241, 332, 484, 610, 934, 1169
Alw21I GWGCWC 2 cut(s) 243, 1018
Alw26I GTCTC 4 cut(s) 125, 441, 1054, 1212
AlwI GGATC 2 cut(s) 1097, 1117
AlwNI CAGNNNCTG 3 cut(s) 625, 1052, 1112
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 4 cut(s) 190, 280, 621, 1192
ApeKI GCWGC 2 cut(s) 457, 900
ApoI RAATTY 2 cut(s) 1082, 1234
Asp700I GAANNNNTTC 2 cut(s) 997, 1356
Asp718I GGTACC 1 cut(s) 1373
AspS9I GGNCC 5 cut(s) 281, 488, 622, 1193, 1343
AsuHPI GGTGA 4 cut(s) 66, 373, 383, 1334
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 2 cut(s) 488, 1343
BaeGI GKGCMC 1 cut(s) 55
BanI GGYRCC 2 cut(s) 50, 1373
BanII GRGCYC 1 cut(s) 243
BarI GAAGNNNNNNTAC 2 cut(s) 1239, 1271
Bbv12I GWGCWC 2 cut(s) 243, 1018
BbvCI CCTCAGC 1 cut(s) 1161
BbvI GCAGC 2 cut(s) 469, 887
BccI CCATC 9 cut(s) 76, 179, 185, 409, 445, 742, 998, 1154, 1370
BceAI ACGGC 1 cut(s) 666
BcgI CGANNNNNNTGC 2 cut(s) 1052, 1086
BciT130I CCWGG 5 cut(s) 462, 492, 620, 716, 1107
BclI TGATCA 1 cut(s) 937
BcoDI GTCTC 4 cut(s) 125, 441, 1054, 1212
BfaI CTAG 3 cut(s) 476, 485, 1097
BfmI CTRYAG 1 cut(s) 1061
BisI GCNGC 2 cut(s) 458, 901
BlsI GCNGC 2 cut(s) 459, 902
Bme1390I CCNGG 5 cut(s) 462, 492, 620, 716, 1107
Bme18I GGWCC 2 cut(s) 488, 1343
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 5 cut(s) 281, 488, 622, 1193, 1343
BmiI GGNNCC 6 cut(s) 52, 364, 720, 1329, 1344, 1375
BmrFI CCNGG 5 cut(s) 462, 492, 620, 716, 1107
BmsI GCATC 3 cut(s) 270, 754, 968
BplI GAGNNNNNCTC 2 cut(s) 489, 521
BpmI CTGGAG 1 cut(s) 596
Bpu10I CCTNAGC 1 cut(s) 1161
BsaBI GATNNNNATC 1 cut(s) 733
BsaJI CCNNGG 2 cut(s) 274, 1216
BsaXI ACNNNNNCTCC 3 cut(s) 30, 594, 624
Bsc4I CCNNNNNNNGG 4 cut(s) 289, 332, 497, 619
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 2 cut(s) 773, 1314
Bse8I GATNNNNATC 1 cut(s) 733
BseBI CCWGG 5 cut(s) 462, 492, 620, 716, 1107
BseDI CCNNGG 2 cut(s) 274, 1216
BseGI GGATG 2 cut(s) 643, 829
BseJI GATNNNNATC 1 cut(s) 733
BseLI CCNNNNNNNGG 4 cut(s) 289, 332, 497, 619
BseMII CTCAG 7 cut(s) 288, 645, 1135, 1152, 1217, 1223, 1303
BseNI ACTGG 2 cut(s) 773, 1314
BseRI GAGGAG 1 cut(s) 323
BseSI GKGCMC 1 cut(s) 55
BseXI GCAGC 2 cut(s) 469, 887
BseYI CCCAGC 1 cut(s) 332
BsgI GTGCAG 2 cut(s) 610, 1249
Bsh1236I CGCG 1 cut(s) 276
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 4 cut(s) 192, 282, 623, 1194
BshNI GGYRCC 2 cut(s) 50, 1373
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 2 cut(s) 243, 1018
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 3 cut(s) 48, 189, 366
BslI CCNNNNNNNGG 4 cut(s) 289, 332, 497, 619
BsmAI GTCTC 4 cut(s) 125, 441, 1054, 1212
BsmI GAATGC 2 cut(s) 211, 1153
BsnI GGCC 4 cut(s) 192, 282, 623, 1194
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 3 cut(s) 55, 243, 1018
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 4 cut(s) 937, 1102, 1109, 1117
BspACI CCGC 3 cut(s) 184, 274, 276
BspANI GGCC 4 cut(s) 192, 282, 623, 1194
BspCNI CTCAG 7 cut(s) 289, 646, 1134, 1153, 1216, 1222, 1302
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 6 cut(s) 52, 364, 720, 1329, 1344, 1375
BspMAI CTGCAG 1 cut(s) 1065
BspPI GGATC 2 cut(s) 1097, 1117
BspT107I GGYRCC 2 cut(s) 50, 1373
BsrBI CCGCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 47
BsrGI TGTACA 1 cut(s) 574
BsrI ACTGG 2 cut(s) 773, 1314
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 274, 1216
BssMI GATC 4 cut(s) 937, 1102, 1109, 1117
BssT1I CCWWGG 1 cut(s) 1216
Bst2UI CCWGG 5 cut(s) 462, 492, 620, 716, 1107
Bst4CI ACNGT 3 cut(s) 350, 667, 1058
Bst6I CTCTTC 2 cut(s) 490, 636
BstAUI TGTACA 1 cut(s) 574
BstC8I GCNNGC 2 cut(s) 702, 1065
BstDEI CTNAG 7 cut(s) 297, 654, 1121, 1161, 1203, 1209, 1289
BstDSI CCRYGG 1 cut(s) 274
BstF5I GGATG 2 cut(s) 643, 829
BstFNI CGCG 1 cut(s) 276
BstKTI GATC 4 cut(s) 940, 1105, 1112, 1120
BstMAI GTCTC 4 cut(s) 125, 441, 1054, 1212
BstMBI GATC 4 cut(s) 937, 1102, 1109, 1117
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 3 cut(s) 247, 454, 1166
BstNI CCWGG 5 cut(s) 462, 492, 620, 716, 1107
BstNSI RCATGY 2 cut(s) 575, 704
BstSCI CCNGG 5 cut(s) 460, 490, 618, 714, 1105
BstSFI CTRYAG 1 cut(s) 1061
BstSLI GKGCMC 1 cut(s) 55
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 2 cut(s) 469, 887
BstX2I RGATCY 1 cut(s) 1109
BstXI CCANNNNNNTGG 1 cut(s) 1369
BstYI RGATCY 1 cut(s) 1109
BsuRI GGCC 4 cut(s) 192, 282, 623, 1194
BtgI CCRYGG 1 cut(s) 274
BtgZI GCGATG 2 cut(s) 446, 730
BtsCI GGATG 2 cut(s) 643, 829
BtsIMutI CAGTG 2 cut(s) 558, 1129
Cac8I GCNNGC 2 cut(s) 702, 1065
CaiI CAGNNNCTG 3 cut(s) 625, 1052, 1112
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 5 cut(s) 281, 488, 622, 1193, 1343
Cfr42I CCGCGG 1 cut(s) 277
CseI GACGC 1 cut(s) 528
CsiI ACCWGGT 1 cut(s) 714
Csp6I GTAC 3 cut(s) 575, 648, 1374
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviQI GTAC 3 cut(s) 575, 648, 1374
DdeI CTNAG 7 cut(s) 297, 654, 1121, 1161, 1203, 1209, 1289
DpnI GATC 4 cut(s) 939, 1104, 1111, 1119
DpnII GATC 4 cut(s) 937, 1102, 1109, 1117
DraIII CACNNNGTG 1 cut(s) 583
Eam1104I CTCTTC 2 cut(s) 490, 636
EarI CTCTTC 2 cut(s) 490, 636
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 1216
Eco24I GRGCYC 1 cut(s) 243
Eco47I GGWCC 2 cut(s) 488, 1343
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 3 cut(s) 708, 852, 1155
Eco88I CYCGRG 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 2 cut(s) 622, 1343
EcoRII CCWGG 5 cut(s) 460, 490, 618, 714, 1105
EcoT14I CCWWGG 1 cut(s) 1216
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 1216
FbaI TGATCA 1 cut(s) 937
Fnu4HI GCNGC 2 cut(s) 458, 901
FokI GGATG 2 cut(s) 650, 836
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 2 cut(s) 458, 901
FspBI CTAG 3 cut(s) 476, 485, 1097
GluI GCNGC 2 cut(s) 458, 901
GsaI CCCAGC 1 cut(s) 336
GsuI CTGGAG 1 cut(s) 596
HaeIII GGCC 4 cut(s) 192, 282, 623, 1194
HapII CCGG 3 cut(s) 48, 189, 366
HgaI GACGC 1 cut(s) 528
HincII GTYRAC 2 cut(s) 972, 1090
HindII GTYRAC 2 cut(s) 972, 1090
HindIII AAGCTT 3 cut(s) 24, 932, 1167
HinfI GANTC 6 cut(s) 133, 233, 563, 836, 973, 1283
HpaII CCGG 3 cut(s) 48, 189, 366
HphI GGTGA 4 cut(s) 66, 373, 383, 1334
Hpy166II GTNNAC 4 cut(s) 98, 577, 972, 1090
Hpy188I TCNGA 9 cut(s) 232, 238, 789, 1003, 1038, 1054, 1074, 1241, 1367
Hpy188III TCNNGA 4 cut(s) 726, 1097, 1173, 1346
Hpy8I GTNNAC 4 cut(s) 98, 577, 972, 1090
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 5 cut(s) 29, 527, 732, 1313, 1342
HpyCH4III ACNGT 3 cut(s) 350, 667, 1058
HpyCH4IV ACGT 1 cut(s) 1131
HpyCH4V TGCA 8 cut(s) 198, 571, 591, 599, 903, 959, 1063, 1266
HpyF10VI GCNNNNNNNGC 3 cut(s) 247, 454, 1166
HpyF3I CTNAG 7 cut(s) 297, 654, 1121, 1161, 1203, 1209, 1289
HpySE526I ACGT 1 cut(s) 1131
KpnI GGTACC 1 cut(s) 1377
Ksp22I TGATCA 1 cut(s) 937
KspI CCGCGG 1 cut(s) 277
Kzo9I GATC 4 cut(s) 937, 1102, 1109, 1117
LmnI GCTCC 6 cut(s) 191, 362, 445, 615, 1043, 1333
Lsp1109I GCAGC 2 cut(s) 469, 887
LweI GCATC 3 cut(s) 270, 754, 968
MabI ACCWGGT 1 cut(s) 714
MaeI CTAG 3 cut(s) 476, 485, 1097
MaeII ACGT 1 cut(s) 1131
MalI GATC 4 cut(s) 939, 1104, 1111, 1119
MbiI CCGCTC 1 cut(s) 186
MboI GATC 4 cut(s) 937, 1102, 1109, 1117
MflI RGATCY 1 cut(s) 1109
MhlI GDGCHC 3 cut(s) 55, 243, 1018
MluCI AATT 7 cut(s) 782, 993, 1023, 1068, 1082, 1234, 1277
MlyI GAGTC 3 cut(s) 127, 227, 967
MmeI TCCRAC 2 cut(s) 255, 1294
MroXI GAANNNNTTC 2 cut(s) 997, 1356
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 2 cut(s) 216, 1367
MspA1I CMGCKG 1 cut(s) 276
MspI CCGG 3 cut(s) 48, 189, 366
MspR9I CCNGG 5 cut(s) 462, 492, 620, 716, 1107
Mva1269I GAATGC 2 cut(s) 211, 1153
MvaI CCWGG 5 cut(s) 462, 492, 620, 716, 1107
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 3 cut(s) 247, 454, 1166
NdeII GATC 4 cut(s) 937, 1102, 1109, 1117
NlaIV GGNNCC 6 cut(s) 52, 364, 720, 1329, 1344, 1375
NspI RCATGY 2 cut(s) 575, 704
PaeI GCATGC 1 cut(s) 704
PctI GAATGC 2 cut(s) 211, 1153
PdmI GAANNNNTTC 2 cut(s) 997, 1356
PfeI GAWTC 3 cut(s) 563, 836, 1283
PfoI TCCNGGA 2 cut(s) 490, 1105
PkrI GCNGC 2 cut(s) 459, 902
PleI GAGTC 3 cut(s) 127, 227, 967
PpsI GAGTC 3 cut(s) 127, 227, 967
PpuMI RGGWCCY 1 cut(s) 1343
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 1343
Psp6I CCWGG 5 cut(s) 460, 490, 618, 714, 1105
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 5 cut(s) 460, 490, 618, 714, 1105
PspN4I GGNNCC 6 cut(s) 52, 364, 720, 1329, 1344, 1375
PspPI GGNCC 5 cut(s) 281, 488, 622, 1193, 1343
PspPPI RGGWCCY 1 cut(s) 1343
PstI CTGCAG 1 cut(s) 1065
PstNI CAGNNNCTG 3 cut(s) 625, 1052, 1112
PsuI RGATCY 1 cut(s) 1109
RsaI GTAC 3 cut(s) 576, 649, 1375
RsaNI GTAC 3 cut(s) 575, 648, 1374
RseI CAYNNNNRTG 2 cut(s) 216, 1367
SacI GAGCTC 1 cut(s) 243
SacII CCGCGG 1 cut(s) 277
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 2 cut(s) 458, 901
Sau3AI GATC 4 cut(s) 937, 1102, 1109, 1117
Sau96I GGNCC 5 cut(s) 281, 488, 622, 1193, 1343
SchI GAGTC 3 cut(s) 127, 227, 967
ScrFI CCNGG 5 cut(s) 462, 492, 620, 716, 1107
SduI GDGCHC 3 cut(s) 55, 243, 1018
SexAI ACCWGGT 1 cut(s) 714
SfaNI GCATC 3 cut(s) 270, 754, 968
SfcI CTRYAG 1 cut(s) 1061
Sfr303I CCGCGG 1 cut(s) 277
SgrBI CCGCGG 1 cut(s) 277
SinI GGWCC 2 cut(s) 488, 1343
SmiMI CAYNNNNRTG 2 cut(s) 216, 1367
SphI GCATGC 1 cut(s) 704
Sse9I AATT 7 cut(s) 782, 993, 1023, 1068, 1082, 1234, 1277
SsiI CCGC 3 cut(s) 184, 274, 276
SspMI CTAG 3 cut(s) 476, 485, 1097
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 5 cut(s) 460, 490, 618, 714, 1105
StyI CCWWGG 1 cut(s) 1216
TaaI ACNGT 3 cut(s) 350, 667, 1058
TaiI ACGT 1 cut(s) 1134
TaqI TCGA 2 cut(s) 180, 711
TasI AATT 7 cut(s) 782, 993, 1023, 1068, 1082, 1234, 1277
TatI WGTACW 1 cut(s) 574
TfiI GAWTC 3 cut(s) 563, 836, 1283
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 2 cut(s) 565, 1129
TseI GCWGC 2 cut(s) 457, 900
TspDTI ATGAA 8 cut(s) 234, 359, 485, 543, 654, 767, 1178, 1398
TspGWI ACGGA 3 cut(s) 836, 980, 1283
TspRI CASTG 2 cut(s) 565, 1129
VpaK11BI GGWCC 2 cut(s) 488, 1343
XapI RAATTY 2 cut(s) 1082, 1234
XbaI TCTAGA 1 cut(s) 1096
XceI RCATGY 2 cut(s) 575, 704
XmnI GAANNNNTTC 2 cut(s) 997, 1356
XspI CTAG 3 cut(s) 476, 485, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.