pycom111g04060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0013201
Physical Location & Seq
Reverse (-)
2470894 .. 2471512
619 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g04060.1

Sequence Viewer

Length: 471 bp
ATGACTACAGCTAAGAAAACCGTTAGACCAAAACTCTCGCCACTGCGCAATCCGTTCCGTGCCGTTTCAACCAACATGATGACACTGCGTCACAAGCCGGAGGAGGAGAACCGGAACCAGAGCGAGATTCGGGAGACGATGGAGGAGGTCCGGCGGAGCATGCTGTCCGTATCGTCGAAGGAGAAGAAGGCGTGTACGGAAGACCAGGAGGAGGACAGCGCGGGATCGTCGGAGGAGATGGAGTCGCCGAGGTCGGTGGGGCGATGGCGGAGGGGGACGACGAAGAAGACGGGGTGCGTTCTGCACAGCCAGATCTTGAGGATCAGAGAGGAGGACTCGCATCTCGGCGAGAGTACGGCTCTGATCGGTGCTGCCGCTGCAGCTGGAAAAGATTATCACGGCATTGACGTCGTTCTTTTCGCCCGGCCAATCTTGCCTTCCTCGCCTCTCAAGAACACAATCAACGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.39

Weight (kDa)

9.23

Isoelectric Point (pI)

82.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15190 AT5G15190
fragaria_vesca FvH4_6g48930 FvH4_6g48930
malus_domestica MD09G1049600.v1.1 MD17G1049800.v1.1
prunus_persica Prupe.3G269200_v2.0.a1
pyrus_communis pycom111g04060 pycom17g04470
rosa_chinensis RchiOBHm_Chr2g0168741
rosa_laevigata RLG00000021806
rosa_multiflora Rmu_sc0012935.1_g000006 Rmu_sc0022948.1_g000001
rosa_roxburghii Rroxscaffold_2G00082720
rosa_rugosa Rorug02G0535200
rosa_samantha Rh2CG587000 Rh2DG629500
rosa_wichuraiana Rw2G050230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 411
Acc16I TGCGCA 1 cut(s) 47
AccII CGCG 1 cut(s) 221
AciI CCGC 4 cut(s) 154, 221, 268, 375
AclWI GGATC 2 cut(s) 232, 329
AcoI YGGCCR 1 cut(s) 425
AcyI GRCGYC 1 cut(s) 408
AfaI GTAC 2 cut(s) 196, 355
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 1 cut(s) 69
AhdI GACNNNNNGTC 1 cut(s) 87
AjnI CCWGG 1 cut(s) 204
AjuI GAANNNNNNNTTGG 2 cut(s) 421, 453
AluBI AGCT 2 cut(s) 11, 383
AluI AGCT 2 cut(s) 11, 383
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 2 cut(s) 232, 329
AoxI GGCC 1 cut(s) 425
ApeKI GCWGC 3 cut(s) 371, 377, 380
AspLEI GCGC 2 cut(s) 48, 221
AspS9I GGNCC 1 cut(s) 148
AsuC2I CCSGG 1 cut(s) 424
AvaII GGWCC 1 cut(s) 148
BbsI GAAGAC 2 cut(s) 207, 293
BbvI GCAGC 3 cut(s) 358, 364, 392
BccI CCATC 3 cut(s) 133, 232, 258
BceAI ACGGC 3 cut(s) 47, 372, 415
BcgI CGANNNNNNTGC 2 cut(s) 391, 425
BciT130I CCWGG 1 cut(s) 206
BcnI CCSGG 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 128
BfmI CTRYAG 2 cut(s) 6, 378
BglII AGATCT 1 cut(s) 312
BisI GCNGC 4 cut(s) 372, 375, 378, 381
BlsI GCNGC 4 cut(s) 373, 376, 379, 382
Bme1390I CCNGG 2 cut(s) 206, 424
Bme18I GGWCC 1 cut(s) 148
BmeRI GACNNNNNGTC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 116
BmrFI CCNGG 2 cut(s) 206, 424
BmsI GCATC 1 cut(s) 349
BpiI GAAGAC 2 cut(s) 207, 293
BplI GAGNNNNNCTC 4 cut(s) 320, 352, 343, 375
BpuEI CTTGAG 2 cut(s) 337, 434
BpuMI CCSGG 1 cut(s) 424
BsaHI GRCGYC 1 cut(s) 408
BsaJI CCNNGG 1 cut(s) 248
BsaWI WCCGGW 1 cut(s) 111
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 211
BseBI CCWGG 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 248
BseLI CCNNNNNNNGG 1 cut(s) 211
BseRI GAGGAG 6 cut(s) 116, 119, 158, 224, 248, 344
BseXI GCAGC 3 cut(s) 358, 364, 392
BsgI GTGCAG 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 1 cut(s) 427
BsiSI CCGG 4 cut(s) 98, 112, 151, 424
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 128
BsmBI CGTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 289
BsnI GGCC 1 cut(s) 427
Bsp143I GATC 4 cut(s) 224, 312, 321, 363
BspACI CCGC 4 cut(s) 154, 221, 268, 375
BspANI GGCC 1 cut(s) 427
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 1 cut(s) 116
BspMAI CTGCAG 1 cut(s) 382
BspPI GGATC 2 cut(s) 232, 329
BssECI CCNNGG 1 cut(s) 248
BssMI GATC 4 cut(s) 224, 312, 321, 363
BssNI GRCGYC 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 2 cut(s) 22, 467
BstACI GRCGYC 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 1 cut(s) 12
BstFNI CGCG 1 cut(s) 221
BstHHI GCGC 2 cut(s) 48, 221
BstKTI GATC 4 cut(s) 227, 315, 324, 366
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 4 cut(s) 224, 312, 321, 363
BstMWI GCNNNNNNNGC 6 cut(s) 94, 160, 377, 380, 433, 442
BstNI CCWGG 1 cut(s) 206
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 2 cut(s) 204, 422
BstSFI CTRYAG 2 cut(s) 6, 378
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 3 cut(s) 358, 364, 392
BstV2I GAAGAC 2 cut(s) 207, 293
BstX2I RGATCY 1 cut(s) 312
BstYI RGATCY 1 cut(s) 312
BsuRI GGCC 1 cut(s) 427
BtgZI GCGATG 1 cut(s) 277
BtsI GCAGTG 2 cut(s) 41, 83
BtsIMutI CAGTG 2 cut(s) 41, 83
Cac8I GCNNGC 1 cut(s) 161
CfoI GCGC 2 cut(s) 48, 221
Cfr13I GGNCC 1 cut(s) 148
CseI GACGC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 195, 354
CviAII CATG 2 cut(s) 76, 160
CviJI RGCY 6 cut(s) 11, 97, 309, 359, 383, 427
CviKI_1 RGCY 6 cut(s) 11, 97, 309, 359, 383, 427
CviQI GTAC 2 cut(s) 195, 354
DdeI CTNAG 1 cut(s) 12
DpnI GATC 4 cut(s) 226, 314, 323, 365
DpnII GATC 4 cut(s) 224, 312, 321, 363
DriI GACNNNNNGTC 1 cut(s) 87
EaeI YGGCCR 1 cut(s) 425
Eam1105I GACNNNNNGTC 1 cut(s) 87
EciI GGCGGA 2 cut(s) 169, 283
Eco47I GGWCC 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 204
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 2 cut(s) 79, 163
FaiI YATR 2 cut(s) 77, 161
FaqI GGGAC 1 cut(s) 289
FatI CATG 2 cut(s) 75, 159
FauI CCCGC 1 cut(s) 214
Fnu4HI GCNGC 4 cut(s) 372, 375, 378, 381
Fsp4HI GCNGC 4 cut(s) 372, 375, 378, 381
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 47, 220
GluI GCNGC 4 cut(s) 372, 375, 378, 381
HaeIII GGCC 1 cut(s) 427
HapII CCGG 4 cut(s) 98, 112, 151, 424
HgaI GACGC 1 cut(s) 77
HhaI GCGC 2 cut(s) 48, 221
Hin1I GRCGYC 1 cut(s) 408
Hin1II CATG 2 cut(s) 79, 163
Hin6I GCGC 2 cut(s) 46, 219
HinP1I GCGC 2 cut(s) 46, 219
HinfI GANTC 3 cut(s) 127, 242, 335
HpaII CCGG 4 cut(s) 98, 112, 151, 424
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 3 cut(s) 232, 326, 363
Hpy188III TCNNGA 3 cut(s) 131, 316, 451
Hpy8I GTNNAC 1 cut(s) 195
Hpy99I CGWCG 4 cut(s) 178, 232, 283, 413
HpyAV CCTTC 3 cut(s) 172, 181, 447
HpyCH4III ACNGT 2 cut(s) 22, 467
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 2 cut(s) 304, 380
HpyF10VI GCNNNNNNNGC 6 cut(s) 94, 160, 377, 380, 433, 442
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 1 cut(s) 408
Hsp92I GRCGYC 1 cut(s) 408
Hsp92II CATG 2 cut(s) 79, 163
HspAI GCGC 2 cut(s) 46, 219
Kzo9I GATC 4 cut(s) 224, 312, 321, 363
LmnI GCTCC 1 cut(s) 156
LpnPI CCDG 9 cut(s) 111, 125, 131, 164, 191, 218, 323, 369, 437
Lsp1109I GCAGC 3 cut(s) 358, 364, 392
LweI GCATC 1 cut(s) 349
MaeII ACGT 1 cut(s) 408
MaeIII GTNAC 1 cut(s) 89
MalI GATC 4 cut(s) 226, 314, 323, 365
MboI GATC 4 cut(s) 224, 312, 321, 363
MboII GAAGA 4 cut(s) 196, 212, 295, 298
MflI RGATCY 1 cut(s) 312
MlyI GAGTC 2 cut(s) 251, 329
MmeI TCCRAC 1 cut(s) 210
MseI TTAA 1 cut(s) 469
MspA1I CMGCKG 2 cut(s) 377, 383
MspI CCGG 4 cut(s) 98, 112, 151, 424
MspR9I CCNGG 2 cut(s) 206, 424
MvaI CCWGG 1 cut(s) 206
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 6 cut(s) 94, 160, 377, 380, 433, 442
NciI CCSGG 1 cut(s) 424
NdeII GATC 4 cut(s) 224, 312, 321, 363
NlaIII CATG 2 cut(s) 79, 163
NlaIV GGNNCC 1 cut(s) 116
NmeAIII GCCGAG 2 cut(s) 273, 324
NmuCI GTSAC 1 cut(s) 89
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 1 cut(s) 163
PaeI GCATGC 1 cut(s) 163
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 4 cut(s) 373, 376, 379, 382
PleI GAGTC 2 cut(s) 250, 329
PpsI GAGTC 2 cut(s) 250, 329
Psp6I CCWGG 1 cut(s) 204
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 1 cut(s) 116
PspPI GGNCC 1 cut(s) 148
PstI CTGCAG 1 cut(s) 382
PsuI RGATCY 1 cut(s) 312
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 2 cut(s) 196, 355
RsaNI GTAC 2 cut(s) 195, 354
SaqAI TTAA 1 cut(s) 469
SatI GCNGC 4 cut(s) 372, 375, 378, 381
Sau3AI GATC 4 cut(s) 224, 312, 321, 363
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 2 cut(s) 251, 329
ScrFI CCNGG 2 cut(s) 206, 424
SetI ASST 5 cut(s) 13, 150, 254, 385, 411
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 2 cut(s) 6, 378
SinI GGWCC 1 cut(s) 148
SmlI CTYRAG 2 cut(s) 316, 449
SmoI CTYRAG 2 cut(s) 316, 449
SphI GCATGC 1 cut(s) 163
SsiI CCGC 4 cut(s) 154, 221, 268, 375
StyD4I CCNGG 2 cut(s) 204, 422
TaaI ACNGT 2 cut(s) 22, 467
TaiI ACGT 1 cut(s) 411
TaqI TCGA 1 cut(s) 176
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 1 cut(s) 469
Tru9I TTAA 1 cut(s) 469
TscAI CASTG 2 cut(s) 48, 90
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 3 cut(s) 371, 377, 380
Tsp45I GTSAC 1 cut(s) 89
TspGWI ACGGA 4 cut(s) 42, 47, 157, 212
TspRI CASTG 2 cut(s) 48, 90
VpaK11BI GGWCC 1 cut(s) 148
XceI RCATGY 1 cut(s) 163
ZraI GACGTC 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.