pycom111g05620

Protein CHLORORESPIRATORY REDUCTION 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0014482
Physical Location & Seq
Reverse (-)
3593413 .. 3594459
1047 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGTTATCTGCAAAATCTTGTTTTGATTCAACATGCTTTTGGATTTGGAATTTTAGTACCAAGAAGCTGAAATTTGCAGGAAAAATGGTTTCAATGGAAGGAGTTTTGAAGAAGCAGTTGTTCCATGGTGGATTTCAGGCCTTTAACTGTAAAGAAGATACAAAGCGCCGGATTCGAGCTCCGTTGATGCAGTTAATGGCAACAGGAAAAAATGATGAGTCTGAGTACATGAGTTCTTCAGTTCATCTTAATCTGTTTAAAACAAGGAGTGTGCAAAGAGATGCAGTCAAGGTTTGTGCAGTGAGGAGGAGAAGGATATACCATAATAGTGAAACTTATGTGTTACTGGAGCCAGGAGAGGATGAGAAGTTTGTTTCAGAGGATGAGCTGAGGGACAAGTTAAAAGGTTGGCTGGAGAACTGGCCAGCCAAGAACCTTCCTCCTGACCTTGCAAGATTCGAAAGCATCGACGACGCCGTTTCTTATCTAGTGAGGTCTGTTTGTGAACTTGAAATTCATGGAGATGTAGGTTCAGTCCAGTGGTACGAAGTTCGAATGGAGTGA

Protein Analysis

188

Amino Acids

21.76

Weight (kDa)

8.23

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRR7 PF12095 106 - 187 2.2e-28 Protein CHLORORESPIRATORY REDUCTION 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39210
fragaria_vesca FvH4_6g46840
malus_domestica MD09G1066900.v1.1
prunus_persica Prupe.3G254800_v2.0.a1
pyrus_communis pycom111g05620 pycom17g05950
rosa_chinensis RchiOBHm_Chr2g0165831
rosa_laevigata RLG00000021560
rosa_multiflora Rmu_sc0003227.1_g000001 Rmu_sc0008709.1_g000001
rosa_roxburghii Rroxscaffold_2G00085460
rosa_rugosa Rorug02G0518200
rosa_samantha Rh2AG584000 Rh2BG595900 Rh2CG566900 Rh2DG605700
rosa_wichuraiana Rw2G048690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 422
AcsI RAATTY 3 cut(s) 49, 71, 513
AcuI CTGAAG 1 cut(s) 222
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 3 cut(s) 58, 227, 545
AgsI TTSAA 4 cut(s) 30, 93, 109, 512
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 3 cut(s) 67, 179, 388
AluI AGCT 3 cut(s) 67, 179, 388
Alw21I GWGCWC 1 cut(s) 181
AoxI GGCC 2 cut(s) 138, 422
ApoI RAATTY 3 cut(s) 49, 71, 513
AspLEI GCGC 1 cut(s) 168
AsuII TTCGAA 2 cut(s) 459, 553
BalI TGGCCA 1 cut(s) 424
BanII GRGCYC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 181
BbvCI CCTCAGC 1 cut(s) 389
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 1 cut(s) 354
BfaI CTAG 1 cut(s) 488
BfoI RGCGCY 1 cut(s) 169
Bme1390I CCNGG 1 cut(s) 354
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 3 cut(s) 177, 271, 474
BpmI CTGGAG 2 cut(s) 368, 434
Bpu10I CCTNAGC 1 cut(s) 389
Bpu14I TTCGAA 2 cut(s) 459, 553
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 124
Bse1I ACTGG 3 cut(s) 351, 425, 538
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 1 cut(s) 124
BseGI GGATG 2 cut(s) 367, 388
BseMII CTCAG 2 cut(s) 213, 380
BseNI ACTGG 3 cut(s) 351, 425, 538
BseRI GAGGAG 2 cut(s) 319, 322
BsgI GTGCAG 1 cut(s) 318
BshFI GGCC 2 cut(s) 140, 424
BsiHKAI GWGCWC 1 cut(s) 181
BsiSI CCGG 1 cut(s) 169
BslFI GGGAC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 407
BsnI GGCC 2 cut(s) 140, 424
Bsp119I TTCGAA 2 cut(s) 459, 553
Bsp1286I GDGCHC 1 cut(s) 181
Bsp19I CCATGG 1 cut(s) 124
BspANI GGCC 2 cut(s) 140, 424
BspCNI CTCAG 2 cut(s) 214, 381
BspLI GGNNCC 1 cut(s) 351
BspT104I TTCGAA 2 cut(s) 459, 553
BsrI ACTGG 3 cut(s) 351, 425, 538
BssECI CCNNGG 1 cut(s) 124
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 124
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 149
BstACI GRCGYC 1 cut(s) 474
BstBI TTCGAA 2 cut(s) 459, 553
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 2 cut(s) 222, 389
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 2 cut(s) 367, 388
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 352
BsuRI GGCC 2 cut(s) 140, 424
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 2 cut(s) 367, 388
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 2 cut(s) 306, 545
Cac8I GCNNGC 1 cut(s) 426
CfoI GCGC 1 cut(s) 168
CseI GACGC 1 cut(s) 482
Csp6I GTAC 3 cut(s) 57, 226, 544
CviAII CATG 4 cut(s) 33, 125, 229, 518
CviJI RGCY 8 cut(s) 67, 140, 179, 352, 388, 412, 424, 428
CviKI_1 RGCY 8 cut(s) 67, 140, 179, 352, 388, 412, 424, 428
CviQI GTAC 3 cut(s) 57, 226, 544
DdeI CTNAG 2 cut(s) 222, 389
DraI TTTAAA 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 422
Ecl136II GAGCTC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 124
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 179
Eco57I CTGAAG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 4 cut(s) 36, 128, 232, 521
FaiI YATR 7 cut(s) 34, 126, 230, 319, 324, 339, 519
FaqI GGGAC 1 cut(s) 407
FatI CATG 4 cut(s) 32, 124, 228, 517
FokI GGATG 2 cut(s) 374, 395
FriOI GRGCYC 1 cut(s) 181
FspBI CTAG 1 cut(s) 488
GlaI GCGC 1 cut(s) 167
GsuI CTGGAG 2 cut(s) 368, 434
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 2 cut(s) 140, 424
HapII CCGG 1 cut(s) 169
HgaI GACGC 1 cut(s) 482
HhaI GCGC 1 cut(s) 168
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 4 cut(s) 36, 128, 232, 521
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HinfI GANTC 4 cut(s) 26, 172, 218, 456
HpaII CCGG 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 506
Hpy188I TCNGA 2 cut(s) 223, 379
Hpy188III TCNNGA 1 cut(s) 443
Hpy8I GTNNAC 1 cut(s) 506
Hpy99I CGWCG 2 cut(s) 473, 476
HpyAV CCTTC 3 cut(s) 92, 306, 446
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4V TGCA 7 cut(s) 11, 77, 190, 274, 284, 299, 452
HpyF3I CTNAG 2 cut(s) 222, 389
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 4 cut(s) 36, 128, 232, 521
HspAI GCGC 1 cut(s) 166
LmnI GCTCC 2 cut(s) 184, 349
LweI GCATC 3 cut(s) 177, 271, 474
MaeI CTAG 1 cut(s) 488
MaeIII GTNAC 1 cut(s) 342
MboII GAAGA 3 cut(s) 121, 167, 228
MhlI GDGCHC 1 cut(s) 181
MlsI TGGCCA 1 cut(s) 424
MluCI AATT 3 cut(s) 49, 71, 513
MluNI TGGCCA 1 cut(s) 424
MlyI GAGTC 1 cut(s) 227
MnlI CCTC 7 cut(s) 297, 300, 352, 373, 384, 450, 486
Mox20I TGGCCA 1 cut(s) 424
MscI TGGCCA 1 cut(s) 424
MseI TTAA 5 cut(s) 144, 194, 249, 258, 401
MslI CAYNNNNRTG 2 cut(s) 327, 522
Msp20I TGGCCA 1 cut(s) 424
MspI CCGG 1 cut(s) 169
MspR9I CCNGG 1 cut(s) 354
MvaI CCWGG 1 cut(s) 354
NcoI CCATGG 1 cut(s) 124
NlaIII CATG 4 cut(s) 36, 128, 232, 521
NlaIV GGNNCC 1 cut(s) 351
NspI RCATGY 1 cut(s) 36
NspV TTCGAA 2 cut(s) 459, 553
PceI AGGCCT 1 cut(s) 140
PcsI WCGNNNNNNNCGW 2 cut(s) 465, 474
PfeI GAWTC 3 cut(s) 26, 172, 456
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 352
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 1 cut(s) 351
RsaI GTAC 3 cut(s) 58, 227, 545
RsaNI GTAC 3 cut(s) 57, 226, 544
RseI CAYNNNNRTG 2 cut(s) 327, 522
SacI GAGCTC 1 cut(s) 181
SaqAI TTAA 5 cut(s) 144, 194, 249, 258, 401
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 1 cut(s) 181
SetI ASST 9 cut(s) 69, 181, 294, 390, 409, 438, 450, 497, 532
SfaNI GCATC 3 cut(s) 177, 271, 474
SfuI TTCGAA 2 cut(s) 459, 553
SmiMI CAYNNNNRTG 2 cut(s) 327, 522
Sse9I AATT 3 cut(s) 49, 71, 513
SseBI AGGCCT 1 cut(s) 140
SspMI CTAG 1 cut(s) 488
SstI GAGCTC 1 cut(s) 181
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 1 cut(s) 149
TaqI TCGA 4 cut(s) 175, 459, 468, 553
TasI AATT 3 cut(s) 49, 71, 513
TatI WGTACW 1 cut(s) 225
TfiI GAWTC 3 cut(s) 26, 172, 456
Tru1I TTAA 5 cut(s) 144, 194, 249, 258, 401
Tru9I TTAA 5 cut(s) 144, 194, 249, 258, 401
TscAI CASTG 2 cut(s) 306, 545
TspDTI ATGAA 2 cut(s) 233, 506
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 2 cut(s) 306, 545
XapI RAATTY 3 cut(s) 49, 71, 513
XceI RCATGY 1 cut(s) 36
XspI CTAG 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.