pycom11g03960

Protein of unknown function (DUF2488)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010918
Physical Location & Seq
Forward (+)
3101268 .. 3103464
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
ATGTACACATCATTTATTATATTATGTAGTATAACACTAGATAAGGCTGGCTTTGCTTATTATATCGAAGGAAGACACGCAGGGGAGGAAGAAGAAGAACCAGGAGGAGCAATGTCTGCCGTGGCAACTCTCGGTCAGGCATGCTGGGCCGCCGCCGCCGCCGTGAAATCGAAACCAACCACCGCCATCTCCCAAACCCACATCCGAAATCCAAAACCCAAATCTTTTTCTCTTCCTCTCTCAGCCAATTCCAGCTGCAACCACTTCTTCTCCTTCCCTCCCAAAACCACCCTCAAATCCTCCTCCTCCGTCCGTACATCTGTCGCCGCCGTCGACTCCGACCAGCTCAGCTCCTCCGATTCCGCCGATAAGGAAAAGCCGAGCAGGTACTATTTCGTGGTTGCGAATGCGAAGTTTATGCTCGATGACGAGGAGCATTTCCAGGAGCAATTGTGTGAGCGGCTTCGATACTATGGAGAGAAGAACAAAGAGCAGGACTTTTGGCTTGTGATCGAGCCCAAGTTCTTGGATAAGTTTCCTAACATTACTAAGAGATTAGGAAGGCCTGCCGTTGCTCTGGTTTCGACTAACGGACCTTGGATTACGTTTATGAAGCTGAGACTGGACAGAGTGTTAGCAGAAAGTTTTGAAGCTGCAAGTCTAGAAGAAGCATTAGCCTCCAATCCAACTAAGATCGATTTTGAGAAGCCGCAGAAATGGGTAGCACCTTATTCCAAGTATGAATCCGGGTGGTGGGAGCCCTTCTTACCGCCAGGATCAAAAGAGGAGGTCAAAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ycf54

Protein Analysis

267

Amino Acids

29.76

Weight (kDa)

6.16

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf54 PF10674 129 - 218 3.5e-35 Ycf54 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 375
AccBSI CCGCTC 1 cut(s) 460
AccI GTMKAC 1 cut(s) 333
AclWI GGATC 1 cut(s) 784
AfaI GTAC 3 cut(s) 5, 316, 389
AfiI CCNNNNNNNGG 1 cut(s) 753
AgsI TTSAA 1 cut(s) 650
AjnI CCWGG 3 cut(s) 100, 441, 772
AluBI AGCT 5 cut(s) 255, 346, 351, 616, 653
AluI AGCT 5 cut(s) 255, 346, 351, 616, 653
Alw26I GTCTC 1 cut(s) 613
AlwI GGATC 1 cut(s) 784
AoxI GGCC 2 cut(s) 147, 563
ApeKI GCWGC 2 cut(s) 255, 653
AspS9I GGNCC 2 cut(s) 147, 593
AsuC2I CCSGG 1 cut(s) 748
AvaII GGWCC 1 cut(s) 593
BanII GRGCYC 2 cut(s) 519, 762
BbsI GAAGAC 1 cut(s) 79
BbvI GCAGC 2 cut(s) 242, 640
BccI CCATC 1 cut(s) 194
BceAI ACGGC 4 cut(s) 104, 146, 314, 554
BcgI CGANNNNNNTGC 2 cut(s) 400, 434
BciT130I CCWGG 3 cut(s) 102, 443, 774
BcnI CCSGG 1 cut(s) 748
BcoDI GTCTC 1 cut(s) 613
BfaI CTAG 2 cut(s) 38, 662
BfuAI ACCTGC 1 cut(s) 375
BisI GCNGC 9 cut(s) 150, 153, 156, 159, 256, 327, 461, 654, 710
BlpI GCTNAGC 1 cut(s) 347
BlsI GCNGC 9 cut(s) 151, 154, 157, 160, 257, 328, 462, 655, 711
Bme1390I CCNGG 4 cut(s) 102, 443, 748, 774
Bme18I GGWCC 1 cut(s) 593
BmgT120I GGNCC 2 cut(s) 147, 593
BmiI GGNNCC 1 cut(s) 759
BmrFI CCNGG 4 cut(s) 102, 443, 748, 774
BpiI GAAGAC 1 cut(s) 79
Bpu1102I GCTNAGC 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 748
Bsa29I ATCGAT 1 cut(s) 696
BsaJI CCNNGG 2 cut(s) 120, 596
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 437, 467
Bsc4I CCNNNNNNNGG 1 cut(s) 753
Bse1I ACTGG 1 cut(s) 627
Bse3DI GCAATG 1 cut(s) 117
BseBI CCWGG 3 cut(s) 102, 443, 774
BseCI ATCGAT 1 cut(s) 696
BseDI CCNNGG 2 cut(s) 120, 596
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 753
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 3 cut(s) 255, 361, 608
BseNI ACTGG 1 cut(s) 627
BseRI GAGGAG 6 cut(s) 120, 292, 295, 343, 446, 800
BseXI GCAGC 2 cut(s) 242, 640
BseYI CCCAGC 1 cut(s) 144
BshFI GGCC 2 cut(s) 149, 565
BshVI ATCGAT 1 cut(s) 696
BsiSI CCGG 1 cut(s) 747
BslI CCNNNNNNNGG 1 cut(s) 753
BsmAI GTCTC 1 cut(s) 613
BsmI GAATGC 1 cut(s) 412
BsnI GGCC 2 cut(s) 149, 565
Bsp1286I GDGCHC 2 cut(s) 519, 762
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 510, 693, 776
Bsp1720I GCTNAGC 1 cut(s) 347
BspANI GGCC 2 cut(s) 149, 565
BspCNI CTCAG 3 cut(s) 254, 360, 609
BspDI ATCGAT 1 cut(s) 696
BspLI GGNNCC 1 cut(s) 759
BspMI ACCTGC 1 cut(s) 375
BspPI GGATC 1 cut(s) 784
BsrBI CCGCTC 1 cut(s) 460
BsrDI GCAATG 1 cut(s) 117
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 627
BssECI CCNNGG 2 cut(s) 120, 596
BssMI GATC 3 cut(s) 510, 693, 776
BssT1I CCWWGG 1 cut(s) 596
Bst2UI CCWGG 3 cut(s) 102, 443, 774
Bst6I CTCTTC 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 116
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 49, 142, 567
BstDEI CTNAG 5 cut(s) 241, 347, 549, 617, 690
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 201
BstKTI GATC 3 cut(s) 513, 696, 779
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 3 cut(s) 510, 693, 776
BstMWI GCNNNNNNNGC 5 cut(s) 53, 116, 146, 155, 158
BstNI CCWGG 3 cut(s) 102, 443, 774
BstNSI RCATGY 1 cut(s) 144
BstSCI CCNGG 4 cut(s) 100, 441, 746, 772
BstV1I GCAGC 2 cut(s) 242, 640
BstV2I GAAGAC 1 cut(s) 79
BstXI CCANNNNNNTGG 1 cut(s) 526
Bsu15I ATCGAT 1 cut(s) 696
BsuRI GGCC 2 cut(s) 149, 565
BsuTUI ATCGAT 1 cut(s) 696
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 201
BveI ACCTGC 1 cut(s) 375
Cac8I GCNNGC 3 cut(s) 49, 142, 567
Cfr13I GGNCC 2 cut(s) 147, 593
ClaI ATCGAT 1 cut(s) 696
Csp6I GTAC 3 cut(s) 4, 315, 388
CviAII CATG 1 cut(s) 141
CviQI GTAC 3 cut(s) 4, 315, 388
DdeI CTNAG 5 cut(s) 241, 347, 549, 617, 690
DpnI GATC 3 cut(s) 512, 695, 778
DpnII GATC 3 cut(s) 510, 693, 776
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
EciI GGCGGA 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 596
Eco147I AGGCCT 1 cut(s) 565
Eco24I GRGCYC 2 cut(s) 519, 762
Eco47I GGWCC 1 cut(s) 593
EcoRII CCWGG 3 cut(s) 100, 441, 772
EcoT14I CCWWGG 1 cut(s) 596
EcoT38I GRGCYC 2 cut(s) 519, 762
ErhI CCWWGG 1 cut(s) 596
FaeI CATG 1 cut(s) 144
FaiI YATR 9 cut(s) 20, 25, 32, 63, 142, 419, 474, 611, 741
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FatI CATG 1 cut(s) 140
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 9 cut(s) 150, 153, 156, 159, 256, 327, 461, 654, 710
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 2 cut(s) 519, 762
Fsp4HI GCNGC 9 cut(s) 150, 153, 156, 159, 256, 327, 461, 654, 710
FspBI CTAG 2 cut(s) 38, 662
GluI GCNGC 9 cut(s) 150, 153, 156, 159, 256, 327, 461, 654, 710
GsaI CCCAGC 1 cut(s) 148
HaeIII GGCC 2 cut(s) 149, 565
HapII CCGG 1 cut(s) 747
Hin1II CATG 1 cut(s) 144
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 3 cut(s) 335, 359, 743
HpaII CCGG 1 cut(s) 747
Hpy166II GTNNAC 2 cut(s) 6, 334
Hpy188I TCNGA 3 cut(s) 206, 340, 358
Hpy188III TCNNGA 1 cut(s) 662
Hpy8I GTNNAC 2 cut(s) 6, 334
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 4 cut(s) 62, 283, 555, 772
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 2 cut(s) 258, 656
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 116, 146, 155, 158
HpyF3I CTNAG 5 cut(s) 241, 347, 549, 617, 690
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 1 cut(s) 144
Kzo9I GATC 3 cut(s) 510, 693, 776
LmnI GCTCC 5 cut(s) 107, 356, 433, 445, 757
Lsp1109I GCAGC 2 cut(s) 242, 640
MaeI CTAG 2 cut(s) 38, 662
MaeII ACGT 1 cut(s) 605
MalI GATC 3 cut(s) 512, 695, 778
MbiI CCGCTC 1 cut(s) 460
MboI GATC 3 cut(s) 510, 693, 776
MboII GAAGA 8 cut(s) 84, 101, 104, 107, 224, 259, 493, 677
MfeI CAATTG 1 cut(s) 449
MhlI GDGCHC 2 cut(s) 519, 762
MluCI AATT 2 cut(s) 247, 449
MlyI GAGTC 1 cut(s) 329
MmeI TCCRAC 2 cut(s) 363, 710
MspA1I CMGCKG 1 cut(s) 255
MspI CCGG 1 cut(s) 747
MspR9I CCNGG 4 cut(s) 102, 443, 748, 774
MunI CAATTG 1 cut(s) 449
Mva1269I GAATGC 1 cut(s) 412
MvaI CCWGG 3 cut(s) 102, 443, 774
MwoI GCNNNNNNNGC 5 cut(s) 53, 116, 146, 155, 158
NciI CCSGG 1 cut(s) 748
NdeII GATC 3 cut(s) 510, 693, 776
NlaIII CATG 1 cut(s) 144
NlaIV GGNNCC 1 cut(s) 759
NmeAIII GCCGAG 1 cut(s) 405
NspI RCATGY 1 cut(s) 144
PaeI GCATGC 1 cut(s) 144
PceI AGGCCT 1 cut(s) 565
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PctI GAATGC 1 cut(s) 412
PfeI GAWTC 2 cut(s) 359, 743
PfoI TCCNGGA 1 cut(s) 441
PkrI GCNGC 9 cut(s) 151, 154, 157, 160, 257, 328, 462, 655, 711
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
Psp6I CCWGG 3 cut(s) 100, 441, 772
PspFI CCCAGC 1 cut(s) 144
PspGI CCWGG 3 cut(s) 100, 441, 772
PspN4I GGNNCC 1 cut(s) 759
PspPI GGNCC 2 cut(s) 147, 593
PvuII CAGCTG 1 cut(s) 255
RsaI GTAC 3 cut(s) 5, 316, 389
RsaNI GTAC 3 cut(s) 4, 315, 388
SalI GTCGAC 1 cut(s) 332
SatI GCNGC 9 cut(s) 150, 153, 156, 159, 256, 327, 461, 654, 710
Sau3AI GATC 3 cut(s) 510, 693, 776
Sau96I GGNCC 2 cut(s) 147, 593
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 4 cut(s) 102, 443, 748, 774
SduI GDGCHC 2 cut(s) 519, 762
SinI GGWCC 1 cut(s) 593
SphI GCATGC 1 cut(s) 144
Sse9I AATT 2 cut(s) 247, 449
SseBI AGGCCT 1 cut(s) 565
SspMI CTAG 2 cut(s) 38, 662
StuI AGGCCT 1 cut(s) 565
StyD4I CCNGG 4 cut(s) 100, 441, 746, 772
StyI CCWWGG 1 cut(s) 596
TaiI ACGT 1 cut(s) 608
TaqI TCGA 8 cut(s) 66, 170, 333, 423, 466, 513, 584, 696
TaqII GACCGA 1 cut(s) 122
TasI AATT 2 cut(s) 247, 449
TatI WGTACW 1 cut(s) 3
TauI GCSGC 7 cut(s) 152, 155, 158, 161, 329, 463, 712
TfiI GAWTC 2 cut(s) 359, 743
TseI GCWGC 2 cut(s) 255, 653
TspDTI ATGAA 2 cut(s) 626, 756
TspGWI ACGGA 3 cut(s) 298, 302, 606
VpaK11BI GGWCC 1 cut(s) 593
XbaI TCTAGA 1 cut(s) 661
XceI RCATGY 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 2 cut(s) 38, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.