pycom11g11180
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011433
Physical Location & Seq
Forward (+)
9738269 .. 9743901
5633 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2502 bp
ATGTCTTCAACTCCAACTCCAACTGCCGAACCGTCCGCAACTGAACTCCTAAACCCACCATCCCCACTCAACGGGCACGGTTCAACCGATCAACCCCAACCGCCACAGGACCCAGCAACCCAACCGCCCGACGACGTCGTATCCGGCGACAAACGCAAGCGAGAGGTTAACGACGACGTCCAAAACCCTTCCGCCGCGCCCCCTCTATGGAAGACCAGCCTCTGCTCCTTTTTCAGGCGCGAGGCCAAAACCTGCGGCCACGGAAGCGAGTGCCGCTACGCACACGGCGAGGCGGAGCTCCGTCCCCGACCCGACAACACGTGGGACCCGACATCCGAGCGCGCCAAGAAGCAGAAGTTGGAGGAGGAGATTGAGGGGTGCGTGGCTTTGGACAATGTGATGATGACGGAGGCCATCGATGAAGACGGAGACGGCGGCGATGCTGGGGGTTTGGATCCCGAGCTGAGCAAGTGCCTGGTGCATTTGCCGAGGAATTGGAAGTCCGAGAAACTGAAAGAATTTCTCAGTGAGAAGGGAGTCGGATTTAAGTCGGCGAAGAAGAAGAAAGGCATGGCTTTAGGTTTTGTGAGTTTTGAGAATGCAGAGCAATTACAAAATGCTGTGAAGGACCTGGAAGGAATATCGATTGGCAACAAAGTTATGAAGGTTGCGGATGTCATTCCCAGATCATTCGAAAAGAGAACGAAATCGATAATGGCACCTCAAATCCCAGGTGTTGGATTAGATGGGGAGAATGTGGAAGACTCTATGACTGCAAATGGGGCTGAGGATGGTGATGGGCTTACTGGACGAAGTGCCCGGAAAGTCGTGACTCCCCTAGCTCATATGTCTTATGATGATCAGTTGGAGCATAAAAAGAGCTCTCTTATGCAGATTCTCAAAAGACTTACGAGGAACGCTCGTAAAGCTTGTCCTGATGGTATTCCACTTCCAGAATGGATACTCAAGTCTAGGGAGATAGGTGGCCTTCCCTGCAGTTTAGAGGGTATACTAGCGTCCCCAGTTGTAAAAGGATATCGGAACAAGTGTGAATTTTCAGTTGGGTATTCTTTACAGGGAAAAATTACTGTGGGGTTTATGCTTGGAAACTTTAGGGAGGGTGTTACAGCAGTTGAAGAACCCTTTGACTGCCCAAATGTTTCCAGAATTTCTTCCCAATATGCTTCAATATTTCAGGAATTTCTGCAACATTCAAGTTTACCAATTTGGAACAGATTTAAGAATATTGGATTCTGGCGCCAATTGACGGTTCGAGAAGGGAGGAAACCAGGCATTGCTACTGATTCTGAACATTGTGAGGCCAATATTATGGAGGTCATGCTTATTGTTCAGGTTTCCAGTAGCGGATTTGATGATGCACAAATAACCAGTGAATATGAGAGGTTGGCTCAAGCTTTTGCTGCAGGAGCTGCTGCTAGCTCTCCATCTTTGCCACTAATGTTTTTAGTTATTCAGGATCACCAAGGAATATCGAATGCAGCTCCAGCTGATGCTCCACTGCGCTCATTATCTATACCTAAAGCAGAAGGTGGTTCCGAAACGCAGTCCGCTGACAATGTTGTAGAACCAAAAATTCATGACTATATAAACAATCTTAAGTTCTGTATATCACCAACAGCCTTTTTCCAGGTTAATACCCCTGCTGCAGAGAAGCTTTACTCGCTTGCGGGGGATTGGGCTCAGTTGGGTCCTGATACCTTGCTATTTGATGTATGTTGTGGGACTGGAACAATTGGGCTCACTTTAGCACACCATGTTGGTATGGTTGTTGGCATTGAAATGAATGCTTCTGCAGTTTCAGATGCTCGTAGGAATGCTGAAATTAATGACATAAAGAACTGTAGATTTGTCTGTGCGAAGGCAGAGGATGTGATGCGGTCTTTGTTGGAAGAGTACGCAATTGCACCTCAGAAACAAGACGATCATGTCACTGAAGGTAGTGACAAAGTTATAGTTACCGAGGGCGAGAAAGCAATCTCAATGGACAACAAACCCAACCCTGAGGAGAGTTCAAGCCATGAGCTTGACACTGCATCTGAGGGTTTAGAAATTGACAAGCAGGAAGTGAGAAGCCAACCTCAAAGCAGTTGCACTTCTGAAAAGGGGAAAACTCCAGTCAAATGTTTCAGTAATGTTGTTGCTATTGTTGATCCTCCACGAAACGGACTTCACCCCACTGTGATCAAAGCACTGAGGACTCATGCTGATCTACGGAAGCTTGTCTACATTTCCTGCAATCCTGAAACGTTGGTGGCAAATGCGATCGAGCTTTGCACACCTTCTCCTGAGAAAATCGACAAGAACAAGAACAACAGAGCATGGAGAAACATGGGCAGTGCTGGCCTAGCACGGCACAGAACCAAGTCTATGCCTGCTTCAGAGCCTTTCCGTCCTGTAAAAGCAATGGCTGTTGACATTTTCCCACATACCGTACACTGTGAATTAGTAATGCTCCTTGAGAGTGTCACCTATGCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 tRNA_U5-meth_tr zf-CCCH

Protein Analysis

834

Amino Acids

90.86

Weight (kDa)

5.59

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 70 - 97 1e-05 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
tRNA_U5-meth_tr PF05958 543 - 636 3.3e-07 tRNA (Uracil-5-)-methyltransferase
Methyltransf_31 PF13847 576 - 636 2.5e-10 Methyltransferase domain
Methyltransf_25 PF13649 577 - 631 3.1e-07 Methyltransferase domain
tRNA_U5-meth_tr PF05958 720 - 764 5.1e-07 tRNA (Uracil-5-)-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1946
AatII GACGTC 2 cut(s) 138, 180
Acc36I ACCTGC 1 cut(s) 260
AccB1I GGYRCC 2 cut(s) 718, 1257
AccB7I CCANNNNNTGG 1 cut(s) 737
AccI GTMKAC 2 cut(s) 1009, 2244
AccII CGCG 3 cut(s) 197, 240, 342
AclI AACGTT 1 cut(s) 2267
AclWI GGATC 4 cut(s) 449, 462, 1485, 2165
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 5 cut(s) 518, 1052, 1167, 1199, 1593
AcuI CTGAAG 2 cut(s) 1974, 2382
AcvI CACGTG 1 cut(s) 321
AcyI GRCGYC 3 cut(s) 135, 177, 1258
AfaI GTAC 2 cut(s) 1916, 2454
AfiI CCNNNNNNNGG 6 cut(s) 71, 207, 234, 737, 1267, 2183
AflII CTTAAG 1 cut(s) 1616
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 7 cut(s) 9, 84, 1136, 1188, 1215, 1799, 2034
AjnI CCWGG 5 cut(s) 474, 630, 730, 1288, 1647
AjuI GAANNNNNNNTTGG 6 cut(s) 341, 373, 1044, 1076, 1254, 1286
Alw21I GWGCWC 2 cut(s) 300, 884
Alw26I GTCTC 1 cut(s) 423
AlwI GGATC 4 cut(s) 449, 462, 1485, 2165
AlwNI CAGNNNCTG 2 cut(s) 222, 1430
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 6 cut(s) 243, 256, 411, 985, 1320, 2362
ApeKI GCWGC 5 cut(s) 1421, 1430, 1433, 1499, 1664
ApoI RAATTY 5 cut(s) 518, 1052, 1167, 1199, 1593
AseI ATTAAT 1 cut(s) 1845
Asp700I GAANNNNTTC 1 cut(s) 1171
AspLEI GCGC 6 cut(s) 199, 240, 342, 344, 1260, 1524
AspS9I GGNCC 4 cut(s) 109, 325, 628, 1709
AsuC2I CCSGG 1 cut(s) 820
AsuHPI GGTGA 5 cut(s) 806, 1472, 1623, 2183, 2479
AsuII TTCGAA 1 cut(s) 693
AsuNHI GCTAGC 1 cut(s) 1436
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 4 cut(s) 109, 325, 628, 1709
AxyI CCTNAGG 1 cut(s) 2022
BaeGI GKGCMC 2 cut(s) 78, 820
BaeI ACNNNNGTAYC 2 cut(s) 953, 986
BamHI GGATCC 1 cut(s) 454
BanI GGYRCC 2 cut(s) 718, 1257
BanII GRGCYC 4 cut(s) 300, 884, 1702, 1761
BarI GAAGNNNNNNTAC 2 cut(s) 2228, 2260
BbrPI CACGTG 1 cut(s) 321
BbsI GAAGAC 3 cut(s) 218, 429, 768
Bbv12I GWGCWC 2 cut(s) 300, 884
BbvCI CCTCAGC 1 cut(s) 786
BbvI GCAGC 5 cut(s) 1408, 1417, 1420, 1511, 1651
BccI CCATC 7 cut(s) 67, 422, 740, 785, 791, 932, 1453
BceAI ACGGC 3 cut(s) 301, 448, 2387
BciT130I CCWGG 5 cut(s) 476, 632, 732, 1290, 1649
BciVI GTATCC 2 cut(s) 151, 954
BclI TGATCA 2 cut(s) 859, 2202
BcnI CCSGG 1 cut(s) 820
BcoDI GTCTC 1 cut(s) 423
BfaI CTAG 5 cut(s) 839, 972, 1013, 1437, 2366
BfmI CTRYAG 5 cut(s) 994, 1422, 1665, 1812, 1861
BfoI RGCGCY 1 cut(s) 1261
BfrI CTTAAG 1 cut(s) 1616
BfuAI ACCTGC 1 cut(s) 260
BfuI GTATCC 2 cut(s) 151, 954
BisI GCNGC 9 cut(s) 195, 256, 274, 436, 1422, 1431, 1434, 1500, 1665
BlpI GCTNAGC 1 cut(s) 464
BlsI GCNGC 9 cut(s) 196, 257, 275, 437, 1423, 1432, 1435, 1501, 1666
Bme1390I CCNGG 6 cut(s) 476, 632, 732, 820, 1290, 1649
Bme18I GGWCC 4 cut(s) 109, 325, 628, 1709
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 4 cut(s) 109, 325, 628, 1709
BmiI GGNNCC 8 cut(s) 111, 326, 327, 456, 720, 1259, 1555, 1710
BmrFI CCNGG 6 cut(s) 476, 632, 732, 820, 1290, 1649
BmrI ACTGGG 1 cut(s) 1016
BmsI GCATC 6 cut(s) 430, 1366, 1501, 1813, 1884, 2063
BmtI GCTAGC 1 cut(s) 1440
BmuI ACTGGG 1 cut(s) 1016
BpiI GAAGAC 3 cut(s) 218, 429, 768
BplI GAGNNNNNCTC 4 cut(s) 904, 936, 1393, 1425
BpmI CTGGAG 2 cut(s) 1488, 2118
Bpu10I CCTNAGC 1 cut(s) 786
Bpu1102I GCTNAGC 1 cut(s) 464
Bpu14I TTCGAA 1 cut(s) 693
BpuEI CTTGAG 3 cut(s) 950, 1395, 2498
BpuMI CCSGG 1 cut(s) 820
Bsa29I ATCGAT 3 cut(s) 417, 644, 710
BsaAI YACGTR 1 cut(s) 321
BsaHI GRCGYC 3 cut(s) 135, 177, 1258
BsaJI CCNNGG 5 cut(s) 259, 488, 730, 1483, 1980
BsaXI ACNNNNNCTCC 3 cut(s) 31, 2287, 2317
Bsc4I CCNNNNNNNGG 6 cut(s) 71, 207, 234, 737, 1267, 2183
Bse1I ACTGG 6 cut(s) 811, 1022, 1359, 1389, 1750, 2135
Bse21I CCTNAGG 1 cut(s) 2022
Bse3DI GCAATG 2 cut(s) 1293, 2430
BseBI CCWGG 5 cut(s) 476, 632, 732, 1290, 1649
BseCI ATCGAT 3 cut(s) 417, 644, 710
BseDI CCNNGG 5 cut(s) 259, 488, 730, 1483, 1980
BseGI GGATG 5 cut(s) 59, 332, 679, 796, 1894
BseLI CCNNNNNNNGG 6 cut(s) 71, 207, 234, 737, 1267, 2183
BseMI GCAATG 2 cut(s) 1293, 2430
BseMII CTCAG 9 cut(s) 455, 538, 777, 1715, 1943, 2013, 2049, 2204, 2298
BseNI ACTGG 6 cut(s) 811, 1022, 1359, 1389, 1750, 2135
BsePI GCGCGC 1 cut(s) 340
BseRI GAGGAG 3 cut(s) 377, 380, 2039
BseSI GKGCMC 2 cut(s) 78, 820
BseXI GCAGC 5 cut(s) 1408, 1417, 1420, 1511, 1651
BseYI CCCAGC 2 cut(s) 112, 443
Bsh1236I CGCG 3 cut(s) 197, 240, 342
Bsh1285I CGRYCG 1 cut(s) 2286
BshFI GGCC 6 cut(s) 245, 258, 413, 987, 1322, 2364
BshNI GGYRCC 2 cut(s) 718, 1257
BshVI ATCGAT 3 cut(s) 417, 644, 710
BsiEI CGRYCG 1 cut(s) 2286
BsiHKAI GWGCWC 2 cut(s) 300, 884
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 2 cut(s) 144, 820
BslFI GGGAC 4 cut(s) 288, 338, 1003, 1756
BslI CCNNNNNNNGG 6 cut(s) 71, 207, 234, 737, 1267, 2183
BsmAI GTCTC 1 cut(s) 423
BsmBI CGTCTC 1 cut(s) 423
BsmFI GGGAC 4 cut(s) 288, 338, 1003, 1756
BsmI GAATGC 4 cut(s) 604, 1501, 1810, 1840
BsnI GGCC 6 cut(s) 245, 258, 413, 987, 1322, 2364
BsoBI CYCGRG 1 cut(s) 458
Bsp119I TTCGAA 1 cut(s) 693
Bsp1286I GDGCHC 6 cut(s) 78, 300, 820, 884, 1702, 1761
Bsp1720I GCTNAGC 1 cut(s) 464
BspANI GGCC 6 cut(s) 245, 258, 413, 987, 1322, 2364
BspCNI CTCAG 9 cut(s) 456, 537, 778, 1714, 1942, 2014, 2050, 2205, 2299
BspDI ATCGAT 3 cut(s) 417, 644, 710
BspFNI CGCG 3 cut(s) 197, 240, 342
BspHI TCATGA 1 cut(s) 1597
BspLI GGNNCC 8 cut(s) 111, 326, 327, 456, 720, 1259, 1555, 1710
BspMAI CTGCAG 4 cut(s) 998, 1426, 1669, 1816
BspMI ACCTGC 1 cut(s) 260
BspOI GCTAGC 1 cut(s) 1440
BspPI GGATC 4 cut(s) 449, 462, 1485, 2165
BspT104I TTCGAA 1 cut(s) 693
BspT107I GGYRCC 2 cut(s) 718, 1257
BspTI CTTAAG 1 cut(s) 1616
BsrDI GCAATG 2 cut(s) 1293, 2430
BsrI ACTGG 6 cut(s) 811, 1022, 1359, 1389, 1750, 2135
BssECI CCNNGG 5 cut(s) 259, 488, 730, 1483, 1980
BssHII GCGCGC 1 cut(s) 340
BssNAI GTATAC 1 cut(s) 1010
BssNI GRCGYC 3 cut(s) 135, 177, 1258
BssT1I CCWWGG 1 cut(s) 1483
Bst1107I GTATAC 1 cut(s) 1010
Bst2UI CCWGG 5 cut(s) 476, 632, 732, 1290, 1649
Bst4CI ACNGT 8 cut(s) 33, 80, 1090, 1270, 1862, 2200, 2452, 2459
Bst6I CTCTTC 1 cut(s) 1905
BstACI GRCGYC 3 cut(s) 135, 177, 1258
BstAFI CTTAAG 1 cut(s) 1616
BstAPI GCANNNNNTGC 1 cut(s) 1430
BstBAI YACGTR 1 cut(s) 321
BstBI TTCGAA 1 cut(s) 693
BstC8I GCNNGC 6 cut(s) 158, 342, 1438, 1686, 2362, 2394
BstDEI CTNAG 9 cut(s) 464, 524, 786, 1701, 1929, 2022, 2058, 2213, 2307
BstDSI CCRYGG 1 cut(s) 259
BstENI CCTNNNNNAGG 1 cut(s) 232
BstF5I GGATG 5 cut(s) 59, 332, 679, 796, 1894
BstFNI CGCG 3 cut(s) 197, 240, 342
BstH2I RGCGCY 1 cut(s) 1261
BstHHI GCGC 6 cut(s) 199, 240, 342, 344, 1260, 1524
BstMAI GTCTC 1 cut(s) 423
BstMCI CGRYCG 1 cut(s) 2286
BstNI CCWGG 5 cut(s) 476, 632, 732, 1290, 1649
BstSCI CCNGG 6 cut(s) 474, 630, 730, 818, 1288, 1647
BstSFI CTRYAG 5 cut(s) 994, 1422, 1665, 1812, 1861
BstSLI GKGCMC 2 cut(s) 78, 820
BstUI CGCG 3 cut(s) 197, 240, 342
BstV1I GCAGC 5 cut(s) 1408, 1417, 1420, 1511, 1651
BstV2I GAAGAC 3 cut(s) 218, 429, 768
BstX2I RGATCY 1 cut(s) 454
BstXI CCANNNNNNTGG 1 cut(s) 1330
BstYI RGATCY 1 cut(s) 454
BstZ17I GTATAC 1 cut(s) 1010
Bsu15I ATCGAT 3 cut(s) 417, 644, 710
Bsu36I CCTNAGG 1 cut(s) 2022
BsuI GTATCC 2 cut(s) 151, 954
BsuRI GGCC 6 cut(s) 245, 258, 413, 987, 1322, 2364
BsuTUI ATCGAT 3 cut(s) 417, 644, 710
BtgI CCRYGG 1 cut(s) 259
BtgZI GCGATG 1 cut(s) 453
BtsCI GGATG 5 cut(s) 59, 332, 679, 796, 1894
BtsI GCAGTG 3 cut(s) 1517, 2049, 2362
BtsIMutI CAGTG 9 cut(s) 532, 1396, 1517, 1950, 2049, 2196, 2210, 2362, 2455
BveI ACCTGC 1 cut(s) 260
Cac8I GCNNGC 6 cut(s) 158, 342, 1438, 1686, 2362, 2394
CaiI CAGNNNCTG 2 cut(s) 222, 1430
CciI TCATGA 1 cut(s) 1597
CfoI GCGC 6 cut(s) 199, 240, 342, 344, 1260, 1524
Cfr13I GGNCC 4 cut(s) 109, 325, 628, 1709
ClaI ATCGAT 3 cut(s) 417, 644, 710
CseI GACGC 1 cut(s) 1005
Csp6I GTAC 2 cut(s) 1915, 2453
CviAII CATG 9 cut(s) 571, 1339, 1598, 1775, 1946, 2039, 2222, 2340, 2350
CviQI GTAC 2 cut(s) 1915, 2453
DdeI CTNAG 9 cut(s) 464, 524, 786, 1701, 1929, 2022, 2058, 2213, 2307
DinI GGCGCC 1 cut(s) 1259
DrdI GACNNNNNNGTC 1 cut(s) 1946
DseDI GACNNNNNNGTC 1 cut(s) 1946
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 1905
EarI CTCTTC 1 cut(s) 1905
EciI GGCGGA 2 cut(s) 181, 308
Ecl136II GAGCTC 2 cut(s) 298, 882
Eco130I CCWWGG 1 cut(s) 1483
Eco24I GRGCYC 4 cut(s) 300, 884, 1702, 1761
Eco32I GATATC 1 cut(s) 1037
Eco47I GGWCC 4 cut(s) 109, 325, 628, 1709
Eco53kI GAGCTC 2 cut(s) 298, 882
Eco57I CTGAAG 2 cut(s) 1974, 2382
Eco72I CACGTG 1 cut(s) 321
Eco81I CCTNAGG 1 cut(s) 2022
Eco88I CYCGRG 1 cut(s) 458
EcoICRI GAGCTC 2 cut(s) 298, 882
EcoNI CCTNNNNNAGG 1 cut(s) 232
EcoO109I RGGNCCY 4 cut(s) 109, 325, 628, 1709
EcoRII CCWGG 5 cut(s) 474, 630, 730, 1288, 1647
EcoRV GATATC 1 cut(s) 1037
EcoT14I CCWWGG 1 cut(s) 1483
EcoT38I GRGCYC 4 cut(s) 300, 884, 1702, 1761
EgeI GGCGCC 1 cut(s) 1259
EheI GGCGCC 1 cut(s) 1259
ErhI CCWWGG 1 cut(s) 1483
Esp3I CGTCTC 1 cut(s) 423
FaeI CATG 9 cut(s) 574, 1342, 1601, 1778, 1949, 2042, 2225, 2343, 2353
FaqI GGGAC 4 cut(s) 288, 338, 1003, 1756
FatI CATG 9 cut(s) 570, 1338, 1597, 1774, 1945, 2038, 2221, 2339, 2349
FauI CCCGC 1 cut(s) 1681
FauNDI CATATG 1 cut(s) 846
FbaI TGATCA 2 cut(s) 859, 2202
FblI GTMKAC 2 cut(s) 1009, 2244
Fnu4HI GCNGC 9 cut(s) 195, 256, 274, 436, 1422, 1431, 1434, 1500, 1665
FokI GGATG 5 cut(s) 46, 319, 686, 803, 1901
FriOI GRGCYC 4 cut(s) 300, 884, 1702, 1761
Fsp4HI GCNGC 9 cut(s) 195, 256, 274, 436, 1422, 1431, 1434, 1500, 1665
FspBI CTAG 5 cut(s) 839, 972, 1013, 1437, 2366
GlaI GCGC 6 cut(s) 198, 239, 341, 343, 1259, 1523
GluI GCNGC 9 cut(s) 195, 256, 274, 436, 1422, 1431, 1434, 1500, 1665
GsaI CCCAGC 2 cut(s) 116, 447
GsuI CTGGAG 2 cut(s) 1488, 2118
HaeII RGCGCY 1 cut(s) 1261
HaeIII GGCC 6 cut(s) 245, 258, 413, 987, 1322, 2364
HapII CCGG 2 cut(s) 144, 820
HgaI GACGC 1 cut(s) 1005
HhaI GCGC 6 cut(s) 199, 240, 342, 344, 1260, 1524
Hin1I GRCGYC 3 cut(s) 135, 177, 1258
Hin1II CATG 9 cut(s) 574, 1342, 1601, 1778, 1949, 2042, 2225, 2343, 2353
Hin6I GCGC 6 cut(s) 197, 238, 340, 342, 1258, 1522
HinP1I GCGC 6 cut(s) 197, 238, 340, 342, 1258, 1522
HincII GTYRAC 2 cut(s) 169, 2434
HindII GTYRAC 2 cut(s) 169, 2434
HindIII AAGCTT 4 cut(s) 927, 1413, 1673, 2237
HinfI GANTC 7 cut(s) 537, 764, 832, 895, 1251, 1304, 2218
HpaI GTTAAC 1 cut(s) 169
HpaII CCGG 2 cut(s) 144, 820
HphI GGTGA 5 cut(s) 806, 1472, 1623, 2183, 2479
Hpy166II GTNNAC 6 cut(s) 169, 1010, 1220, 2245, 2434, 2455
Hpy8I GTNNAC 6 cut(s) 169, 1010, 1220, 2245, 2434, 2455
Hpy99I CGWCG 5 cut(s) 134, 137, 140, 176, 179
HpyCH4III ACNGT 8 cut(s) 33, 80, 1090, 1270, 1862, 2200, 2452, 2459
HpyCH4IV ACGT 4 cut(s) 135, 177, 320, 2267
HpyF3I CTNAG 9 cut(s) 464, 524, 786, 1701, 1929, 2022, 2058, 2213, 2307
HpySE526I ACGT 4 cut(s) 135, 177, 320, 2267
Hsp92I GRCGYC 3 cut(s) 135, 177, 1258
Hsp92II CATG 9 cut(s) 574, 1342, 1601, 1778, 1949, 2042, 2225, 2343, 2353
HspAI GCGC 6 cut(s) 197, 238, 340, 342, 1258, 1522
KasI GGCGCC 1 cut(s) 1257
KflI GGGWCCC 1 cut(s) 325
Ksp22I TGATCA 2 cut(s) 859, 2202
KspAI GTTAAC 1 cut(s) 169
LmnI GCTCC 8 cut(s) 230, 295, 303, 868, 1427, 1507, 1519, 2478
Lsp1109I GCAGC 5 cut(s) 1408, 1417, 1420, 1511, 1651
LweI GCATC 6 cut(s) 430, 1366, 1501, 1813, 1884, 2063
MaeI CTAG 5 cut(s) 839, 972, 1013, 1437, 2366
MaeII ACGT 4 cut(s) 135, 177, 320, 2267
MaeIII GTNAC 6 cut(s) 829, 1123, 1948, 1961, 1975, 2485
MboII GAAGA 9 cut(s) 223, 434, 568, 571, 574, 773, 1148, 1164, 1922
MfeI CAATTG 3 cut(s) 1262, 1752, 1920
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 6 cut(s) 78, 300, 820, 884, 1702, 1761
Mly113I GGCGCC 1 cut(s) 1258
MlyI GAGTC 4 cut(s) 546, 758, 826, 2212
MmeI TCCRAC 7 cut(s) 38, 44, 339, 520, 718, 846, 1887
MroXI GAANNNNTTC 1 cut(s) 1171
MseI TTAA 6 cut(s) 168, 546, 1239, 1617, 1653, 1845
MslI CAYNNNNRTG 1 cut(s) 1799
MspA1I CMGCKG 2 cut(s) 1508, 1571
MspCI CTTAAG 1 cut(s) 1616
MspI CCGG 2 cut(s) 144, 820
MspR9I CCNGG 6 cut(s) 476, 632, 732, 820, 1290, 1649
MunI CAATTG 3 cut(s) 1262, 1752, 1920
Mva1269I GAATGC 4 cut(s) 604, 1501, 1810, 1840
MvaI CCWGG 5 cut(s) 476, 632, 732, 1290, 1649
MvnI CGCG 3 cut(s) 197, 240, 342
NarI GGCGCC 1 cut(s) 1258
NciI CCSGG 1 cut(s) 820
NdeI CATATG 1 cut(s) 846
NheI GCTAGC 1 cut(s) 1436
NlaIII CATG 9 cut(s) 574, 1342, 1601, 1778, 1949, 2042, 2225, 2343, 2353
NlaIV GGNNCC 8 cut(s) 111, 326, 327, 456, 720, 1259, 1555, 1710
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 4 cut(s) 829, 1948, 1961, 2485
NspV TTCGAA 1 cut(s) 693
PagI TCATGA 1 cut(s) 1597
PauI GCGCGC 1 cut(s) 340
PcsI WCGNNNNNNNCGW 4 cut(s) 84, 144, 285, 326
PctI GAATGC 4 cut(s) 604, 1501, 1810, 1840
PdmI GAANNNNTTC 1 cut(s) 1171
PfeI GAWTC 3 cut(s) 895, 1251, 1304
PflFI GACNNNGTC 2 cut(s) 134, 176
PflMI CCANNNNNTGG 1 cut(s) 737
PkrI GCNGC 9 cut(s) 196, 257, 275, 437, 1423, 1432, 1435, 1501, 1666
Ple19I CGATCG 1 cut(s) 2286
PleI GAGTC 4 cut(s) 545, 758, 826, 2212
PluTI GGCGCC 1 cut(s) 1261
PmaCI CACGTG 1 cut(s) 321
PmlI CACGTG 1 cut(s) 321
PpsI GAGTC 4 cut(s) 545, 758, 826, 2212
Ppu21I YACGTR 1 cut(s) 321
PpuMI RGGWCCY 4 cut(s) 109, 325, 628, 1709
PshBI ATTAAT 1 cut(s) 1845
Psp124BI GAGCTC 2 cut(s) 300, 884
Psp1406I AACGTT 1 cut(s) 2267
Psp5II RGGWCCY 4 cut(s) 109, 325, 628, 1709
Psp6I CCWGG 5 cut(s) 474, 630, 730, 1288, 1647
PspCI CACGTG 1 cut(s) 321
PspFI CCCAGC 2 cut(s) 112, 443
PspGI CCWGG 5 cut(s) 474, 630, 730, 1288, 1647
PspN4I GGNNCC 8 cut(s) 111, 326, 327, 456, 720, 1259, 1555, 1710
PspPI GGNCC 4 cut(s) 109, 325, 628, 1709
PspPPI RGGWCCY 4 cut(s) 109, 325, 628, 1709
PstI CTGCAG 4 cut(s) 998, 1426, 1669, 1816
PstNI CAGNNNCTG 2 cut(s) 222, 1430
PsuI RGATCY 1 cut(s) 454
PsyI GACNNNGTC 2 cut(s) 134, 176
PteI GCGCGC 1 cut(s) 340
PvuI CGATCG 1 cut(s) 2286
PvuII CAGCTG 1 cut(s) 1508
RsaI GTAC 2 cut(s) 1916, 2454
RsaNI GTAC 2 cut(s) 1915, 2453
RseI CAYNNNNRTG 1 cut(s) 1799
SacI GAGCTC 2 cut(s) 300, 884
SaqAI TTAA 6 cut(s) 168, 546, 1239, 1617, 1653, 1845
SatI GCNGC 9 cut(s) 195, 256, 274, 436, 1422, 1431, 1434, 1500, 1665
Sau96I GGNCC 4 cut(s) 109, 325, 628, 1709
SchI GAGTC 4 cut(s) 546, 758, 826, 2212
ScrFI CCNGG 6 cut(s) 476, 632, 732, 820, 1290, 1649
SduI GDGCHC 6 cut(s) 78, 300, 820, 884, 1702, 1761
SfaNI GCATC 6 cut(s) 430, 1366, 1501, 1813, 1884, 2063
SfcI CTRYAG 5 cut(s) 994, 1422, 1665, 1812, 1861
SfoI GGCGCC 1 cut(s) 1259
SfuI TTCGAA 1 cut(s) 693
SinI GGWCC 4 cut(s) 109, 325, 628, 1709
SmiMI CAYNNNNRTG 1 cut(s) 1799
SmlI CTYRAG 4 cut(s) 965, 1410, 1616, 2477
SmoI CTYRAG 4 cut(s) 965, 1410, 1616, 2477
SspDI GGCGCC 1 cut(s) 1257
SspI AATATT 3 cut(s) 1191, 1246, 1327
SspMI CTAG 5 cut(s) 839, 972, 1013, 1437, 2366
SstI GAGCTC 2 cut(s) 300, 884
StyD4I CCNGG 6 cut(s) 474, 630, 730, 818, 1288, 1647
StyI CCWWGG 1 cut(s) 1483
TaaI ACNGT 8 cut(s) 33, 80, 1090, 1270, 1862, 2200, 2452, 2459
TaiI ACGT 4 cut(s) 138, 180, 323, 2270
TaqI TCGA 8 cut(s) 417, 644, 693, 710, 1273, 1493, 2286, 2316
TauI GCSGC 4 cut(s) 197, 258, 276, 438
TfiI GAWTC 3 cut(s) 895, 1251, 1304
Tru1I TTAA 6 cut(s) 168, 546, 1239, 1617, 1653, 1845
Tru9I TTAA 6 cut(s) 168, 546, 1239, 1617, 1653, 1845
TscAI CASTG 9 cut(s) 532, 1396, 1524, 1957, 2056, 2203, 2217, 2362, 2462
TseFI GTSAC 4 cut(s) 829, 1948, 1961, 2485
TseI GCWGC 5 cut(s) 1421, 1430, 1433, 1499, 1664
Tsp45I GTSAC 4 cut(s) 829, 1948, 1961, 2485
TspDTI ATGAA 4 cut(s) 435, 677, 1586, 1817
TspGWI ACGGA 7 cut(s) 276, 290, 422, 441, 2199, 2248, 2399
TspRI CASTG 9 cut(s) 532, 1396, 1524, 1957, 2056, 2203, 2217, 2362, 2462
Tth111I GACNNNGTC 2 cut(s) 134, 176
Van91I CCANNNNNTGG 1 cut(s) 737
Vha464I CTTAAG 1 cut(s) 1616
VpaK11BI GGWCC 4 cut(s) 109, 325, 628, 1709
VspI ATTAAT 1 cut(s) 1845
XagI CCTNNNNNAGG 1 cut(s) 232
XapI RAATTY 5 cut(s) 518, 1052, 1167, 1199, 1593
XcmI CCANNNNNNNNNTGG 1 cut(s) 954
XmiI GTMKAC 2 cut(s) 1009, 2244
XmnI GAANNNNTTC 1 cut(s) 1171
XspI CTAG 5 cut(s) 839, 972, 1013, 1437, 2366
ZraI GACGTC 2 cut(s) 136, 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.