pycom11g12230

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0014836
Physical Location & Seq
Forward (+)
11131969 .. 11132447
479 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 372 bp
ATGGCGGACTCGAAAACCAACGAGGTGTTTGCAAATGGGTCAGAAAATCAGGAAAAGCCGGCGTCTTTCGCTAAGACGCTGACCCAATCTGACGCCAACAATGGCGGTGGATTCTTTGTCCCAAGGTACTGCGCTGAGACCATTTTTCCAAGGTTGGATTACTCGGCGGACCCACCGGTGCAGACGGTGATCGCGAAGGACGTTCACGGCGAGGTGTGGAAGTTCATGCACATTTACAGAGGGAGGGACGCCATATCGAAGAAATTGGTGATTGGAGACTCGATTGTGTTTCTGAGAGCTAAAAATGGCGATCTCTATGTTGGGATTTGGAGGGTGAAAAGGGGAATAGATGACGTGGAGGAAATGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_resp B3

Protein Analysis

124

Amino Acids

13.77

Weight (kDa)

6.1

Isoelectric Point (pI)

27.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 23 - 111 2e-08 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 194
AciI CCGC 3 cut(s) 5, 105, 167
AcyI GRCGYC 3 cut(s) 62, 93, 249
AfaI GTAC 1 cut(s) 128
AgeI ACCGGT 1 cut(s) 175
AjiI CACGTC 1 cut(s) 355
AluBI AGCT 1 cut(s) 299
AluI AGCT 1 cut(s) 299
Alw26I GTCTC 2 cut(s) 131, 270
AsiGI ACCGGT 1 cut(s) 175
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 3 cut(s) 199, 280, 346
AvaII GGWCC 1 cut(s) 169
BceAI ACGGC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 11, 45
BcoDI GTCTC 2 cut(s) 131, 270
Bme18I GGWCC 1 cut(s) 169
BmgBI CACGTC 1 cut(s) 355
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 1 cut(s) 171
BsaHI GRCGYC 3 cut(s) 62, 93, 249
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 2 cut(s) 122, 149
BsaWI WCCGGW 1 cut(s) 175
Bse118I RCCGGY 2 cut(s) 58, 175
BseDI CCNNGG 2 cut(s) 122, 149
BseMII CTCAG 2 cut(s) 126, 284
BsgI GTGCAG 1 cut(s) 200
Bsh1236I CGCG 1 cut(s) 194
BshTI ACCGGT 1 cut(s) 175
BsiSI CCGG 2 cut(s) 59, 176
BslFI GGGAC 2 cut(s) 104, 260
BsmAI GTCTC 2 cut(s) 131, 270
BsmFI GGGAC 2 cut(s) 104, 260
Bso31I GGTCTC 1 cut(s) 131
Bsp143I GATC 2 cut(s) 189, 310
Bsp68I TCGCGA 1 cut(s) 194
BspACI CCGC 3 cut(s) 5, 105, 167
BspCNI CTCAG 2 cut(s) 127, 285
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 171
BspTNI GGTCTC 1 cut(s) 131
BsrFI RCCGGY 2 cut(s) 58, 175
BssAI RCCGGY 2 cut(s) 58, 175
BssECI CCNNGG 2 cut(s) 122, 149
BssMI GATC 2 cut(s) 189, 310
BssNI GRCGYC 3 cut(s) 62, 93, 249
BssT1I CCWWGG 2 cut(s) 122, 149
Bst4CI ACNGT 1 cut(s) 187
BstACI GRCGYC 3 cut(s) 62, 93, 249
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 3 cut(s) 72, 135, 293
BstFNI CGCG 1 cut(s) 194
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 2 cut(s) 192, 313
BstMAI GTCTC 2 cut(s) 131, 270
BstMBI GATC 2 cut(s) 189, 310
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstUI CGCG 1 cut(s) 194
BtrI CACGTC 1 cut(s) 355
BtuMI TCGCGA 1 cut(s) 194
Cac8I GCNNGC 1 cut(s) 60
CfoI GCGC 1 cut(s) 134
Cfr10I RCCGGY 2 cut(s) 58, 175
Cfr13I GGNCC 1 cut(s) 169
CseI GACGC 4 cut(s) 51, 85, 101, 257
Csp6I GTAC 1 cut(s) 127
CspAI ACCGGT 1 cut(s) 175
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviAII CATG 1 cut(s) 226
CviJI RGCY 2 cut(s) 58, 299
CviKI_1 RGCY 2 cut(s) 58, 299
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 3 cut(s) 72, 135, 293
DpnI GATC 2 cut(s) 191, 312
DpnII GATC 2 cut(s) 189, 310
EciI GGCGGA 2 cut(s) 20, 182
Eco130I CCWWGG 2 cut(s) 122, 149
Eco31I GGTCTC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 169
EcoT14I CCWWGG 2 cut(s) 122, 149
ErhI CCWWGG 2 cut(s) 122, 149
FaeI CATG 1 cut(s) 229
FaiI YATR 3 cut(s) 227, 254, 318
FaqI GGGAC 2 cut(s) 104, 260
FatI CATG 1 cut(s) 225
GlaI GCGC 1 cut(s) 133
HapII CCGG 2 cut(s) 59, 176
HgaI GACGC 4 cut(s) 51, 85, 101, 257
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 3 cut(s) 62, 93, 249
Hin1II CATG 1 cut(s) 229
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HinfI GANTC 3 cut(s) 8, 111, 278
HpaII CCGG 2 cut(s) 59, 176
HphI GGTGA 3 cut(s) 199, 280, 346
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 3 cut(s) 43, 91, 294
Hpy188III TCNNGA 2 cut(s) 50, 193
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4IV ACGT 2 cut(s) 201, 354
HpyCH4V TGCA 3 cut(s) 32, 181, 229
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 3 cut(s) 72, 135, 293
HpySE526I ACGT 2 cut(s) 201, 354
Hsp92I GRCGYC 3 cut(s) 62, 93, 249
Hsp92II CATG 1 cut(s) 229
HspAI GCGC 1 cut(s) 132
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Kzo9I GATC 2 cut(s) 189, 310
LpnPI CCDG 3 cut(s) 35, 72, 189
MaeII ACGT 2 cut(s) 201, 354
MalI GATC 2 cut(s) 191, 312
MboI GATC 2 cut(s) 189, 310
MboII GAAGA 1 cut(s) 271
MluCI AATT 2 cut(s) 263, 367
MlyI GAGTC 2 cut(s) 2, 272
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 6 cut(s) 16, 205, 233, 237, 324, 352
MroNI GCCGGC 1 cut(s) 58
MspI CCGG 2 cut(s) 59, 176
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 1 cut(s) 68
NaeI GCCGGC 1 cut(s) 60
NdeII GATC 2 cut(s) 189, 310
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 1 cut(s) 229
NlaIV GGNNCC 1 cut(s) 171
NmeAIII GCCGAG 1 cut(s) 143
NruI TCGCGA 1 cut(s) 194
PcsI WCGNNNNNNNCGW 3 cut(s) 191, 198, 207
PdiI GCCGGC 1 cut(s) 60
PfeI GAWTC 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 175
PleI GAGTC 2 cut(s) 2, 272
PpsI GAGTC 2 cut(s) 2, 272
PspN4I GGNNCC 1 cut(s) 171
PspPI GGNCC 1 cut(s) 169
RruI TCGCGA 1 cut(s) 194
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
Sau3AI GATC 2 cut(s) 189, 310
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 2 cut(s) 2, 272
SetI ASST 7 cut(s) 27, 128, 155, 204, 216, 301, 357
SgrAI CRCCGGYG 1 cut(s) 175
SinI GGWCC 1 cut(s) 169
Sse9I AATT 2 cut(s) 263, 367
SsiI CCGC 3 cut(s) 5, 105, 167
StyI CCWWGG 2 cut(s) 122, 149
TaaI ACNGT 1 cut(s) 187
TaiI ACGT 2 cut(s) 204, 357
TaqI TCGA 3 cut(s) 11, 257, 281
TasI AATT 2 cut(s) 263, 367
TfiI GAWTC 1 cut(s) 111
TspDTI ATGAA 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.