Rroxscaffold_1G00058990

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014836
Physical Location & Seq
Forward (+)
80992777 .. 80993352
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058990.1

Sequence Viewer

Length: 576 bp
ATGAGGTCTTCACCCAGAATTTGGCTAGTCCCTTCCGAAAGCAGCGAGGTTTTTGCTGAAGACCAAGGCTATGTGGACTCTGATGGGTCTGACAATCTAGAAAAGCCTTCTTCTTTCGCTAAAACTCTGACCCGGTCTGACGCCAACAATGGCAGCGGCTTCTCTGTCTCGAGGTACTGTGCCGAGACCATTTTTCCGAGGCTCGACTACTCCACCGACCGGCTGATGCAGACCGTGGCTACCAAGGACGTCTATGGCGAGGTGTTGAAGTTTCGACACATTTACCGAGGCACCCCTTGTCGGCATTTGCTGATGACCGGGTGGAGCACGTTCATGAACCAGAAGAAGCTCGTTGCTGGCAACTCAATTGTGTTCTTGCGAGCCCAAAACGGCGACCTCTGTGTCGAGATTTGGAGGGCCAAGCGAGGATCGCAGAAGGGGTCGTCGAAATCGGGGTGGAACCACAGTGGTTCTAGGGGAGCGTCGTTTTTTTCGTCTTCCAGGTTCTCAGTGTTTTTGAAATGGGGAGAAATCATGGAGAGAGAAATCAAGGAGAGAGAAACCAAGTTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_resp B3

Protein Analysis

191

Amino Acids

21.65

Weight (kDa)

9.45

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 39 - 140 5.7e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 401
AatII GACGTC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 290
AccB7I CCANNNNNTGG 1 cut(s) 21
AciI CCGC 1 cut(s) 156
AclWI GGATC 1 cut(s) 436
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 2 cut(s) 141, 249
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 3 cut(s) 21, 219, 300
AgsI TTSAA 2 cut(s) 268, 520
AjnI CCWGG 1 cut(s) 500
AluBI AGCT 1 cut(s) 349
AluI AGCT 1 cut(s) 349
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 2 cut(s) 172, 179
AlwI GGATC 1 cut(s) 436
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 1 cut(s) 417
ApeKI GCWGC 2 cut(s) 42, 153
ApoI RAATTY 1 cut(s) 18
AspS9I GGNCC 1 cut(s) 417
AsuC2I CCSGG 2 cut(s) 133, 319
AsuHPI GGTGA 1 cut(s) 3
AvaI CYCGRG 1 cut(s) 169
BanI GGYRCC 1 cut(s) 290
BanII GRGCYC 1 cut(s) 385
BbsI GAAGAC 2 cut(s) 66, 489
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 2 cut(s) 54, 165
BccI CCATC 1 cut(s) 77
BceAI ACGGC 1 cut(s) 406
BcgI CGANNNNNNTGC 2 cut(s) 35, 69
BciT130I CCWGG 1 cut(s) 502
BcnI CCSGG 2 cut(s) 133, 319
BcoDI GTCTC 2 cut(s) 172, 179
BfaI CTAG 3 cut(s) 26, 98, 474
BisI GCNGC 3 cut(s) 43, 154, 157
BlsI GCNGC 3 cut(s) 44, 155, 158
Bme1390I CCNGG 3 cut(s) 133, 319, 502
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 417
BmiI GGNNCC 2 cut(s) 292, 461
BmrFI CCNGG 3 cut(s) 133, 319, 502
BmsI GCATC 1 cut(s) 216
BpiI GAAGAC 2 cut(s) 66, 489
BpuMI CCSGG 2 cut(s) 133, 319
BsaHI GRCGYC 2 cut(s) 141, 249
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 5 cut(s) 64, 197, 234, 243, 286
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 219, 300
Bse118I RCCGGY 1 cut(s) 219
BseBI CCWGG 1 cut(s) 502
BseDI CCNNGG 5 cut(s) 64, 197, 234, 243, 286
BseLI CCNNNNNNNGG 3 cut(s) 21, 219, 300
BseMII CTCAG 1 cut(s) 522
BseXI GCAGC 2 cut(s) 54, 165
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 1 cut(s) 419
BshNI GGYRCC 1 cut(s) 290
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 329
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 3 cut(s) 133, 220, 318
BslFI GGGAC 1 cut(s) 14
BslI CCNNNNNNNGG 3 cut(s) 21, 219, 300
BsmAI GTCTC 2 cut(s) 172, 179
BsmFI GGGAC 1 cut(s) 14
BsnI GGCC 1 cut(s) 419
Bso31I GGTCTC 1 cut(s) 179
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 329, 385
Bsp143I GATC 1 cut(s) 428
BspACI CCGC 1 cut(s) 156
BspANI GGCC 1 cut(s) 419
BspCNI CTCAG 1 cut(s) 521
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 292, 461
BspPI GGATC 1 cut(s) 436
BspT107I GGYRCC 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 179
BsrFI RCCGGY 1 cut(s) 219
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 5 cut(s) 64, 197, 234, 243, 286
BssMI GATC 1 cut(s) 428
BssNI GRCGYC 2 cut(s) 141, 249
BssT1I CCWWGG 2 cut(s) 64, 243
Bst2UI CCWGG 1 cut(s) 502
Bst4CI ACNGT 3 cut(s) 179, 235, 467
BstACI GRCGYC 2 cut(s) 141, 249
BstC8I GCNNGC 2 cut(s) 358, 381
BstDEI CTNAG 1 cut(s) 508
BstDSI CCRYGG 1 cut(s) 234
BstKTI GATC 1 cut(s) 431
BstMAI GTCTC 2 cut(s) 172, 179
BstMBI GATC 1 cut(s) 428
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstNI CCWGG 1 cut(s) 502
BstSCI CCNGG 3 cut(s) 131, 317, 500
BstV1I GCAGC 2 cut(s) 54, 165
BstV2I GAAGAC 2 cut(s) 66, 489
BsuRI GGCC 1 cut(s) 419
BtgI CCRYGG 1 cut(s) 234
BtsIMutI CAGTG 2 cut(s) 472, 516
Cac8I GCNNGC 2 cut(s) 358, 381
CciI TCATGA 1 cut(s) 333
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 417
CseI GACGC 2 cut(s) 149, 471
Csp6I GTAC 1 cut(s) 175
CviAII CATG 2 cut(s) 334, 535
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 1 cut(s) 508
DpnI GATC 1 cut(s) 430
DpnII GATC 1 cut(s) 428
DrdI GACNNNNNNGTC 1 cut(s) 401
DseDI GACNNNNNNGTC 1 cut(s) 401
Eco130I CCWWGG 2 cut(s) 64, 243
Eco24I GRGCYC 1 cut(s) 385
Eco31I GGTCTC 1 cut(s) 179
Eco57I CTGAAG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 500
EcoT14I CCWWGG 2 cut(s) 64, 243
EcoT38I GRGCYC 1 cut(s) 385
ErhI CCWWGG 2 cut(s) 64, 243
FaeI CATG 2 cut(s) 337, 538
FaiI YATR 4 cut(s) 72, 255, 335, 536
FaqI GGGAC 1 cut(s) 14
FatI CATG 2 cut(s) 333, 534
Fnu4HI GCNGC 3 cut(s) 43, 154, 157
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 3 cut(s) 43, 154, 157
FspBI CTAG 3 cut(s) 26, 98, 474
GluI GCNGC 3 cut(s) 43, 154, 157
HaeIII GGCC 1 cut(s) 419
HapII CCGG 3 cut(s) 133, 220, 318
HgaI GACGC 2 cut(s) 149, 471
Hin1I GRCGYC 2 cut(s) 141, 249
Hin1II CATG 2 cut(s) 337, 538
HinfI GANTC 1 cut(s) 77
HpaII CCGG 3 cut(s) 133, 220, 318
HphI GGTGA 1 cut(s) 3
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 6 cut(s) 37, 82, 91, 129, 139, 198
Hpy188III TCNNGA 4 cut(s) 98, 169, 334, 406
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 2 cut(s) 448, 487
HpyAV CCTTC 3 cut(s) 42, 117, 430
HpyCH4III ACNGT 3 cut(s) 179, 235, 467
HpyCH4IV ACGT 2 cut(s) 249, 329
HpyCH4V TGCA 2 cut(s) 229, 571
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 1 cut(s) 508
HpySE526I ACGT 2 cut(s) 249, 329
Hsp92I GRCGYC 2 cut(s) 141, 249
Hsp92II CATG 2 cut(s) 337, 538
Kzo9I GATC 1 cut(s) 428
LmnI GCTCC 2 cut(s) 324, 479
LpnPI CCDG 8 cut(s) 28, 146, 233, 331, 342, 353, 487, 514
Lsp1109I GCAGC 2 cut(s) 54, 165
LweI GCATC 1 cut(s) 216
MaeI CTAG 3 cut(s) 26, 98, 474
MaeII ACGT 2 cut(s) 249, 329
MalI GATC 1 cut(s) 430
MboI GATC 1 cut(s) 428
MboII GAAGA 4 cut(s) 71, 102, 355, 489
MfeI CAATTG 1 cut(s) 366
MhlI GDGCHC 2 cut(s) 329, 385
MluCI AATT 2 cut(s) 18, 366
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 8 cut(s) 40, 165, 192, 253, 281, 407, 408, 419
MslI CAYNNNNRTG 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 156
MspI CCGG 3 cut(s) 133, 220, 318
MspR9I CCNGG 3 cut(s) 133, 319, 502
MunI CAATTG 1 cut(s) 366
MvaI CCWGG 1 cut(s) 502
MwoI GCNNNNNNNGC 1 cut(s) 430
NciI CCSGG 2 cut(s) 133, 319
NdeII GATC 1 cut(s) 428
NlaIII CATG 2 cut(s) 337, 538
NlaIV GGNNCC 2 cut(s) 292, 461
NmeAIII GCCGAG 1 cut(s) 208
PaeR7I CTCGAG 1 cut(s) 169
PagI TCATGA 1 cut(s) 333
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 491
PflFI GACNNNGTC 1 cut(s) 133
PflMI CCANNNNNTGG 1 cut(s) 21
PkrI GCNGC 3 cut(s) 44, 155, 158
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 500
PspGI CCWGG 1 cut(s) 500
PspN4I GGNNCC 2 cut(s) 292, 461
PspPI GGNCC 1 cut(s) 417
PsyI GACNNNGTC 1 cut(s) 133
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
RseI CAYNNNNRTG 1 cut(s) 332
SatI GCNGC 3 cut(s) 43, 154, 157
Sau3AI GATC 1 cut(s) 428
Sau96I GGNCC 1 cut(s) 417
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 3 cut(s) 133, 319, 502
SduI GDGCHC 2 cut(s) 329, 385
SetI ASST 9 cut(s) 8, 51, 176, 252, 264, 332, 351, 399, 506
SfaNI GCATC 1 cut(s) 216
Sfr274I CTCGAG 1 cut(s) 169
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse9I AATT 2 cut(s) 18, 366
SsiI CCGC 1 cut(s) 156
SspMI CTAG 3 cut(s) 26, 98, 474
StyD4I CCNGG 3 cut(s) 131, 317, 500
StyI CCWWGG 2 cut(s) 64, 243
TaaI ACNGT 3 cut(s) 179, 235, 467
TaiI ACGT 2 cut(s) 252, 332
TaqI TCGA 5 cut(s) 170, 204, 274, 405, 446
TasI AATT 2 cut(s) 18, 366
TauI GCSGC 1 cut(s) 159
TscAI CASTG 2 cut(s) 472, 516
TseI GCWGC 2 cut(s) 42, 153
TspDTI ATGAA 2 cut(s) 322, 350
TspRI CASTG 2 cut(s) 472, 516
Tth111I GACNNNGTC 1 cut(s) 133
Van91I CCANNNNNTGG 1 cut(s) 21
XapI RAATTY 1 cut(s) 18
XbaI TCTAGA 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 169
XspI CTAG 3 cut(s) 26, 98, 474
ZraI GACGTC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.