Rmu_sc0029984.1_g000001

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0029984.1
OG0014836
Physical Location & Seq
Reverse (-)
788 .. 1539
752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0029984.1_g000001.1.cds

Sequence Viewer

Length: 636 bp
atgttgaaacccggtgaccgtactctcagtcttagtctctccgagatgattgtcagtgctactgccacagatactagcagcgaagcaagggtgacgccctcgcaaccgagcgaagctaaagatacgcaaaagtcctttcccgaaaggctagagaagaaccctgtggcgaggttggtgctctcctcgtccacaacgcttgaagaagaagtcaggtcaagggactcccccaacgactgcaccccacggtgctggcacgcctgcgcaatcacacgctcaaaagagacagtttgcacaccaactggttttggagccaaacactcggaaaccgaccagctattcgccaggatccgactcatcccttttgagagcaacaagcgttttgctcaagaccaaggctctatggactctgatggatttgataatccggaaaagccttcttctttcgctaaaactctaacacagtccgacgccaacaatggctgcgacttcttcgtctcgaggtactgtgccgaaaccattttcccgaggctcgactactccgccgaccagccgatgcagacgaccgggtggagcacgttcgtgaaccaaaagaagctcatcgccaacaactcgattgtgttcctacatgcgagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_resp B3

Protein Analysis

211

Amino Acids

23.46

Weight (kDa)

5.96

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
Acc16I TGCGCA 1 cut(s) 262
AccIII TCCGGA 1 cut(s) 424
AciI CCGC 1 cut(s) 540
AclWI GGATC 2 cut(s) 340, 353
AcyI GRCGYC 2 cut(s) 95, 468
AfaI GTAC 2 cut(s) 22, 503
AgsI TTSAA 2 cut(s) 7, 200
AjnI CCWGG 1 cut(s) 341
AleI CACNNNNGTG 1 cut(s) 578
AluBI AGCT 3 cut(s) 116, 334, 595
AluI AGCT 3 cut(s) 116, 334, 595
Alw21I GWGCWC 2 cut(s) 180, 575
Alw26I GTCTC 3 cut(s) 41, 275, 499
AlwI GGATC 2 cut(s) 340, 353
Ama87I CYCGRG 2 cut(s) 496, 523
Aor13HI TCCGGA 1 cut(s) 424
ApeKI GCWGC 2 cut(s) 78, 480
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
AspLEI GCGC 1 cut(s) 263
AsuC2I CCSGG 2 cut(s) 12, 565
AsuHPI GGTGA 2 cut(s) 26, 103
AvaI CYCGRG 2 cut(s) 496, 523
BamHI GGATCC 1 cut(s) 345
Bbv12I GWGCWC 2 cut(s) 180, 575
BbvI GCAGC 2 cut(s) 90, 467
BccI CCATC 1 cut(s) 404
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 1 cut(s) 343
BcnI CCSGG 2 cut(s) 12, 565
BcoDI GTCTC 3 cut(s) 41, 275, 499
BfaI CTAG 2 cut(s) 75, 149
BisI GCNGC 2 cut(s) 79, 481
BlsI GCNGC 2 cut(s) 80, 482
Bme1390I CCNGG 3 cut(s) 12, 343, 565
BmeT110I CYCGRG 2 cut(s) 496, 523
BmiI GGNNCC 2 cut(s) 310, 347
BmrFI CCNGG 3 cut(s) 12, 343, 565
BmsI GCATC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 369
BpuMI CCSGG 2 cut(s) 12, 565
BsaHI GRCGYC 2 cut(s) 95, 468
BsaJI CCNNGG 3 cut(s) 242, 391, 524
BsaWI WCCGGW 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 304
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 1 cut(s) 343
BseDI CCNNGG 3 cut(s) 242, 391, 524
BseGI GGATG 1 cut(s) 354
BseMII CTCAG 1 cut(s) 40
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 1 cut(s) 172
BseXI GCAGC 2 cut(s) 90, 467
BsgI GTGCAG 1 cut(s) 220
Bsh1285I CGRYCG 1 cut(s) 564
BsiEI CGRYCG 1 cut(s) 564
BsiHKAI GWGCWC 2 cut(s) 180, 575
BsiHKCI CYCGRG 2 cut(s) 496, 523
BsiSI CCGG 3 cut(s) 12, 425, 564
BslFI GGGAC 1 cut(s) 233
BsmAI GTCTC 3 cut(s) 41, 275, 499
BsmBI CGTCTC 1 cut(s) 499
BsmFI GGGAC 1 cut(s) 233
BsoBI CYCGRG 2 cut(s) 496, 523
Bsp1286I GDGCHC 2 cut(s) 180, 575
Bsp13I TCCGGA 1 cut(s) 424
Bsp143I GATC 1 cut(s) 345
BspACI CCGC 1 cut(s) 540
BspCNI CTCAG 1 cut(s) 39
BspEI TCCGGA 1 cut(s) 424
BspLI GGNNCC 2 cut(s) 310, 347
BspPI GGATC 2 cut(s) 340, 353
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 3 cut(s) 242, 391, 524
BssMI GATC 1 cut(s) 345
BssNI GRCGYC 2 cut(s) 95, 468
BssT1I CCWWGG 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 5 cut(s) 20, 246, 286, 462, 506
BstACI GRCGYC 2 cut(s) 95, 468
BstC8I GCNNGC 3 cut(s) 251, 255, 259
BstDEI CTNAG 2 cut(s) 26, 32
BstDSI CCRYGG 1 cut(s) 242
BstEII GGTNACC 1 cut(s) 14
BstF5I GGATG 1 cut(s) 354
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 1 cut(s) 348
BstMAI GTCTC 3 cut(s) 41, 275, 499
BstMBI GATC 1 cut(s) 345
BstMCI CGRYCG 1 cut(s) 564
BstNI CCWGG 1 cut(s) 343
BstNSI RCATGY 1 cut(s) 629
BstPI GGTNACC 1 cut(s) 14
BstSCI CCNGG 3 cut(s) 10, 341, 563
BstV1I GCAGC 2 cut(s) 90, 467
BstX2I RGATCY 1 cut(s) 345
BstXI CCANNNNNNTGG 1 cut(s) 249
BstYI RGATCY 1 cut(s) 345
BtgI CCRYGG 1 cut(s) 242
BtgZI GCGATG 1 cut(s) 583
BtsCI GGATG 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 61
Cac8I GCNNGC 3 cut(s) 251, 255, 259
CfoI GCGC 1 cut(s) 263
CseI GACGC 2 cut(s) 103, 476
Csp6I GTAC 2 cut(s) 21, 502
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviAII CATG 1 cut(s) 626
CviQI GTAC 2 cut(s) 21, 502
DdeI CTNAG 2 cut(s) 26, 32
DpnI GATC 1 cut(s) 347
DpnII GATC 1 cut(s) 345
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
EciI GGCGGA 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 391
Eco88I CYCGRG 2 cut(s) 496, 523
Eco91I GGTNACC 1 cut(s) 14
EcoO65I GGTNACC 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 341
EcoT14I CCWWGG 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 391
Esp3I CGTCTC 1 cut(s) 499
FaeI CATG 1 cut(s) 629
FaiI YATR 2 cut(s) 401, 627
FaqI GGGAC 1 cut(s) 233
FatI CATG 1 cut(s) 625
Fnu4HI GCNGC 2 cut(s) 79, 481
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 2 cut(s) 79, 481
FspBI CTAG 2 cut(s) 75, 149
FspI TGCGCA 1 cut(s) 262
GlaI GCGC 1 cut(s) 262
GluI GCNGC 2 cut(s) 79, 481
HapII CCGG 3 cut(s) 12, 425, 564
HgaI GACGC 2 cut(s) 103, 476
HhaI GCGC 1 cut(s) 263
Hin1I GRCGYC 2 cut(s) 95, 468
Hin1II CATG 1 cut(s) 629
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HinfI GANTC 3 cut(s) 221, 351, 404
HpaII CCGG 3 cut(s) 12, 425, 564
HphI GGTGA 2 cut(s) 26, 103
Hpy166II GTNNAC 2 cut(s) 189, 583
Hpy188I TCNGA 5 cut(s) 43, 322, 350, 409, 466
Hpy188III TCNNGA 6 cut(s) 140, 386, 425, 496, 523, 580
Hpy8I GTNNAC 2 cut(s) 189, 583
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 1 cut(s) 444
HpyCH4III ACNGT 5 cut(s) 20, 246, 286, 462, 506
HpyCH4IV ACGT 1 cut(s) 575
HpyCH4V TGCA 3 cut(s) 237, 291, 556
HpyF3I CTNAG 2 cut(s) 26, 32
HpySE526I ACGT 1 cut(s) 575
Hsp92I GRCGYC 2 cut(s) 95, 468
Hsp92II CATG 1 cut(s) 629
HspAI GCGC 1 cut(s) 261
Kpn2I TCCGGA 1 cut(s) 424
Kzo9I GATC 1 cut(s) 345
LmnI GCTCC 2 cut(s) 308, 570
Lsp1109I GCAGC 2 cut(s) 90, 467
LweI GCATC 1 cut(s) 543
MaeI CTAG 2 cut(s) 75, 149
MaeII ACGT 1 cut(s) 575
MaeIII GTNAC 2 cut(s) 14, 91
MalI GATC 1 cut(s) 347
MboI GATC 1 cut(s) 345
MboII GAAGA 5 cut(s) 166, 212, 215, 429, 481
MflI RGATCY 1 cut(s) 345
MhlI GDGCHC 2 cut(s) 180, 575
MlyI GAGTC 3 cut(s) 215, 345, 398
MmeI TCCRAC 2 cut(s) 373, 489
MnlI CCTC 5 cut(s) 109, 162, 193, 492, 519
MroI TCCGGA 1 cut(s) 424
MslI CAYNNNNRTG 1 cut(s) 578
MspI CCGG 3 cut(s) 12, 425, 564
MspR9I CCNGG 3 cut(s) 12, 343, 565
MvaI CCWGG 1 cut(s) 343
NciI CCSGG 2 cut(s) 12, 565
NdeII GATC 1 cut(s) 345
NlaIII CATG 1 cut(s) 629
NlaIV GGNNCC 2 cut(s) 310, 347
NmuCI GTSAC 2 cut(s) 14, 91
NsbI TGCGCA 1 cut(s) 262
NspI RCATGY 1 cut(s) 629
OliI CACNNNNGTG 1 cut(s) 578
PaeR7I CTCGAG 1 cut(s) 496
PkrI GCNGC 2 cut(s) 80, 482
PleI GAGTC 3 cut(s) 215, 345, 398
PpsI GAGTC 3 cut(s) 215, 345, 398
Psp6I CCWGG 1 cut(s) 341
PspEI GGTNACC 1 cut(s) 14
PspGI CCWGG 1 cut(s) 341
PspN4I GGNNCC 2 cut(s) 310, 347
PsuI RGATCY 1 cut(s) 345
RsaI GTAC 2 cut(s) 22, 503
RsaNI GTAC 2 cut(s) 21, 502
RseI CAYNNNNRTG 1 cut(s) 578
SatI GCNGC 2 cut(s) 79, 481
Sau3AI GATC 1 cut(s) 345
SchI GAGTC 3 cut(s) 215, 345, 398
ScrFI CCNGG 3 cut(s) 12, 343, 565
SduI GDGCHC 2 cut(s) 180, 575
SetI ASST 7 cut(s) 118, 173, 215, 336, 503, 578, 597
SfaNI GCATC 1 cut(s) 543
Sfr274I CTCGAG 1 cut(s) 496
SlaI CTCGAG 1 cut(s) 496
SmiMI CAYNNNNRTG 1 cut(s) 578
SmlI CTYRAG 2 cut(s) 384, 496
SmoI CTYRAG 2 cut(s) 384, 496
SsiI CCGC 1 cut(s) 540
SspMI CTAG 2 cut(s) 75, 149
StyD4I CCNGG 3 cut(s) 10, 341, 563
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 5 cut(s) 20, 246, 286, 462, 506
TaiI ACGT 1 cut(s) 578
TaqI TCGA 3 cut(s) 497, 531, 611
TscAI CASTG 1 cut(s) 61
TseFI GTSAC 2 cut(s) 14, 91
TseI GCWGC 2 cut(s) 78, 480
Tsp45I GTSAC 2 cut(s) 14, 91
TspRI CASTG 1 cut(s) 61
XceI RCATGY 1 cut(s) 629
XhoI CTCGAG 1 cut(s) 496
XspI CTAG 2 cut(s) 75, 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.