pycom11g12540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000928
Physical Location & Seq
Forward (+)
11459363 .. 11465394
6032 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1794 bp
ATGAAAAATTATTTAAATGTGGTTTTCTTCTTCCCCAAAAAATTTGAAAACAGGGGGAAACCTAACCAGACAAACAGAAGGGCTCGTCTGAAGCTGGCGGTAATGGCGCTGGACTCCAATTCAATACCTCTACAGGACCAAGAACAACAACAACCACAATTACAAGACCACAACGCAACCGCACACCACCCTTCTGCTCCCCCTGATGAGCTGTTTGACATCTCAACAACAGTAGATCCTAGTTATGTGATCTCTCTGATACGGAGACTTCTACCAGCTAATGCAAACAATAAACACAATTCGTCGGGTTTTGAGGCCCTTGTGCAAGAATTAAATGCTGATTCCATGGAAAAGAGTGAGCCCACTCCACCTGGAGACATATCACTGCACTCATCAAAAGATGTATCTGAAAGCATGGACATTATTGACGATTTTCATAAAAATGCTCCGGAGGAAGGAGAAAATGTTGATTTAAATGGTGTCTCAGTGGGAATAGAGGCTTGGGAAGAGTATGGTTGCATTTTATGGGATCTTGCTGCAAGTAAAACTCATGCAGAACTCATGGTGCAAAACCTTGTACTTGAAGTGCTTTTAGCAAACCTTATGGTTTCACAATCTGTGCGTGCTACGGAAATCAGCCTTGGAATTATTGGAAACCTTGCCTGTCACGAAGTTCCCATGAAACAAATAGTTTCTACAAATGGATTAATTGGAATAATTGTGGATCAATTGTTTTCTGATGATGCTCAGTGTTTATGTGAAACCTGCAGGCTGTTAACTGTGGGTCTTCATAGTAGTGAACGTATTACTTGGGCCAAGGCATTGCAGTCCGAACACAATCTGAGCCGCATTCTATGGATTGCAGAAAATAGCTTAAATCTACAGCTTATAGAAAAGAATGTTGAACTCTTGTTAGCCACCATTGAAAACTCGGACGAAGTTGTGCATATTCTCCTTCCACCTTTAATGAAGCTGGGAGTAGCAAGTCTACTCATAAATCTCTTGGATTTCGAAATGAGCAAATTAATGGGTGAAAGAGAACCTGAAAGGTACCCAATTCTTGATGTAATCCTCCGTTCCATTGAAGCTCTCTCAGTTATTGATGGTCATTCCCAACACATCTGTCCAAACAAGAAACTTTTTCAGCTAGCTTGTGACCTAGTCAAACTCCCTGATAAAGTTGAGGTGTCAAATTCTTGTGTTACAGCTGCAATTCTAATTGCGAACATTCTTTCAGATGAATCTACTCTAGCATTGGAGATAGCGGAGGGTAAACTGTTTATGTTTCCTTCACTTGTTTATTCCTTCCAAGATTTACTATCATGTGCTTTTGGTTTAATATCACGGCACGACATGGAAGTGACACTTCGTATCCATCCAGATTTGCCTTTCTTACAGGGTTTGCTTGATTTATTTCCCTTTTCTTCGGACGATTGGGAAGCAAGGAGTGCACTCTGGAACATCATTGCAAGGTTAATGGTTCGAGTTAAGGAAAAGGAAATGAGCCCGTCAGTTCTCCAGCAGTATGTTTTGGTTCTAGTCAACAAATCTGATGCGATTGAAGATGATCTTCTTGACTTTCAATTAGGCGACTTACAGTCAAAAGCTAGAACTACAGCTCTTAGAAGGATCGTCAGTATTTTAAATCAATGGACTACTTCAAAGGATTATGCCAAGGATAATGACACAACCGACGAACATGCAGATGATTTAAGTGTTGATAGATTGCTGGACTGCTGTCGCAAGCATTCTGAGCATGAACCGTCTACAGTTTCTCAGGGTACGGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

598

Amino Acids

66.77

Weight (kDa)

4.79

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 773
Acc65I GGTACC 1 cut(s) 1050
AccB1I GGYRCC 1 cut(s) 1050
AccI GTMKAC 2 cut(s) 988, 1766
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 4 cut(s) 98, 180, 847, 1265
AclWI GGATC 4 cut(s) 230, 537, 732, 1637
AcsI RAATTY 2 cut(s) 41, 1192
AcuI CTGAAG 1 cut(s) 110
AfaI GTAC 3 cut(s) 579, 1052, 1783
AfiI CCNNNNNNNGG 1 cut(s) 455
AgsI TTSAA 9 cut(s) 47, 123, 584, 905, 926, 1085, 1562, 1583, 1662
AhdI GACNNNNNGTC 1 cut(s) 1597
AjnI CCWGG 1 cut(s) 370
AjuI GAANNNNNNNTTGG 4 cut(s) 484, 516, 624, 656
Alw21I GWGCWC 1 cut(s) 1453
Alw26I GTCTC 3 cut(s) 259, 369, 487
Alw44I GTGCAC 1 cut(s) 1449
AlwI GGATC 4 cut(s) 230, 537, 732, 1637
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 2 cut(s) 315, 813
ApaLI GTGCAC 1 cut(s) 1449
ApeKI GCWGC 2 cut(s) 536, 1208
ApoI RAATTY 2 cut(s) 41, 1192
AseI ATTAAT 2 cut(s) 707, 1025
Asp718I GGTACC 1 cut(s) 1050
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 3 cut(s) 136, 316, 813
AsuHPI GGTGA 1 cut(s) 1043
AsuII TTCGAA 1 cut(s) 1011
AsuNHI GCTAGC 1 cut(s) 1147
AvaII GGWCC 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 1453
BanI GGYRCC 1 cut(s) 1050
BanII GRGCYC 3 cut(s) 85, 363, 1508
BbsI GAAGAC 1 cut(s) 779
Bbv12I GWGCWC 1 cut(s) 1453
BbvI GCAGC 2 cut(s) 523, 1195
BccI CCATC 2 cut(s) 1097, 1383
BceAI ACGGC 1 cut(s) 1361
BcgI CGANNNNNNTGC 2 cut(s) 1682, 1716
BciT130I CCWGG 1 cut(s) 372
BciVI GTATCC 1 cut(s) 1382
BcoDI GTCTC 3 cut(s) 259, 369, 487
BfaI CTAG 6 cut(s) 240, 1148, 1160, 1250, 1538, 1608
BfmI CTRYAG 5 cut(s) 131, 766, 881, 1614, 1767
BfoI RGCGCY 1 cut(s) 110
BfuAI ACCTGC 1 cut(s) 773
BfuI GTATCC 1 cut(s) 1382
BisI GCNGC 3 cut(s) 537, 847, 1209
BlsI GCNGC 3 cut(s) 538, 848, 1210
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 1 cut(s) 136
BmeRI GACNNNNNGTC 1 cut(s) 1597
BmgT120I GGNCC 3 cut(s) 136, 316, 813
BmiI GGNNCC 1 cut(s) 1052
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 2 cut(s) 733, 1543
BmtI GCTAGC 1 cut(s) 1151
BoxI GACNNNNGTC 1 cut(s) 1737
BpiI GAAGAC 1 cut(s) 779
BpmI CTGGAG 2 cut(s) 393, 1502
Bpu14I TTCGAA 1 cut(s) 1011
BsaJI CCNNGG 4 cut(s) 345, 640, 816, 1674
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 2 cut(s) 450, 480
Bsc4I CCNNNNNNNGG 1 cut(s) 455
Bse3DI GCAATG 2 cut(s) 821, 1464
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 4 cut(s) 345, 640, 816, 1674
BseGI GGATG 1 cut(s) 1375
BseLI CCNNNNNNNGG 1 cut(s) 455
BseMI GCAATG 2 cut(s) 821, 1464
BseMII CTCAG 6 cut(s) 498, 761, 833, 1107, 1743, 1790
BseSI GKGCMC 1 cut(s) 1453
BseXI GCAGC 2 cut(s) 523, 1195
BseYI CCCAGC 1 cut(s) 973
BsgI GTGCAG 1 cut(s) 371
BshFI GGCC 2 cut(s) 317, 815
BshNI GGYRCC 1 cut(s) 1050
BsiHKAI GWGCWC 1 cut(s) 1453
BsiSI CCGG 1 cut(s) 449
BslI CCNNNNNNNGG 1 cut(s) 455
BsmAI GTCTC 3 cut(s) 259, 369, 487
BsmI GAATGC 2 cut(s) 849, 1747
BsnI GGCC 2 cut(s) 317, 815
Bsp119I TTCGAA 1 cut(s) 1011
Bsp1286I GDGCHC 4 cut(s) 85, 363, 1453, 1508
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 6 cut(s) 235, 249, 529, 724, 1567, 1629
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 4 cut(s) 98, 180, 847, 1265
BspANI GGCC 2 cut(s) 317, 815
BspCNI CTCAG 6 cut(s) 497, 760, 834, 1106, 1744, 1789
BspEI TCCGGA 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 1052
BspMAI CTGCAG 1 cut(s) 770
BspMI ACCTGC 1 cut(s) 773
BspOI GCTAGC 1 cut(s) 1151
BspPI GGATC 4 cut(s) 230, 537, 732, 1637
BspT104I TTCGAA 1 cut(s) 1011
BspT107I GGYRCC 1 cut(s) 1050
BsrDI GCAATG 2 cut(s) 821, 1464
BssECI CCNNGG 4 cut(s) 345, 640, 816, 1674
BssMI GATC 6 cut(s) 235, 249, 529, 724, 1567, 1629
BssT1I CCWWGG 4 cut(s) 345, 640, 816, 1674
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 6 cut(s) 232, 781, 1278, 1599, 1764, 1771
Bst6I CTCTTC 1 cut(s) 501
BstAPI GCANNNNNTGC 1 cut(s) 1448
BstBI TTCGAA 1 cut(s) 1011
BstC8I GCNNGC 5 cut(s) 96, 624, 770, 1149, 1745
BstDEI CTNAG 7 cut(s) 484, 747, 842, 1093, 1622, 1752, 1776
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 1 cut(s) 1375
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 6 cut(s) 238, 252, 532, 727, 1570, 1632
BstMAI GTCTC 3 cut(s) 259, 369, 487
BstMBI GATC 6 cut(s) 235, 249, 529, 724, 1567, 1629
BstMWI GCNNNNNNNGC 3 cut(s) 104, 1448, 1753
BstNI CCWGG 1 cut(s) 372
BstNSI RCATGY 1 cut(s) 1703
BstPAI GACNNNNGTC 1 cut(s) 1737
BstSCI CCNGG 1 cut(s) 370
BstSFI CTRYAG 5 cut(s) 131, 766, 881, 1614, 1767
BstSLI GKGCMC 1 cut(s) 1453
BstV1I GCAGC 2 cut(s) 523, 1195
BstV2I GAAGAC 1 cut(s) 779
BstX2I RGATCY 2 cut(s) 235, 529
BstYI RGATCY 2 cut(s) 235, 529
BsuI GTATCC 1 cut(s) 1382
BsuRI GGCC 2 cut(s) 317, 815
BtgI CCRYGG 1 cut(s) 345
BtsCI GGATG 1 cut(s) 1375
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 3 cut(s) 383, 492, 755
BveI ACCTGC 1 cut(s) 773
Cac8I GCNNGC 5 cut(s) 96, 624, 770, 1149, 1745
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 3 cut(s) 136, 316, 813
Csp6I GTAC 3 cut(s) 578, 1051, 1782
CviAII CATG 9 cut(s) 346, 415, 551, 562, 679, 1323, 1354, 1700, 1757
CviQI GTAC 3 cut(s) 578, 1051, 1782
DdeI CTNAG 7 cut(s) 484, 747, 842, 1093, 1622, 1752, 1776
DpnI GATC 6 cut(s) 237, 251, 531, 726, 1569, 1631
DpnII GATC 6 cut(s) 235, 249, 529, 724, 1567, 1629
DraI TTTAAA 3 cut(s) 15, 474, 1644
DriI GACNNNNNGTC 1 cut(s) 1597
Eam1104I CTCTTC 1 cut(s) 501
Eam1105I GACNNNNNGTC 1 cut(s) 1597
EarI CTCTTC 1 cut(s) 501
Eco130I CCWWGG 4 cut(s) 345, 640, 816, 1674
Eco24I GRGCYC 3 cut(s) 85, 363, 1508
Eco47I GGWCC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 370
EcoT14I CCWWGG 4 cut(s) 345, 640, 816, 1674
EcoT38I GRGCYC 3 cut(s) 85, 363, 1508
ErhI CCWWGG 4 cut(s) 345, 640, 816, 1674
FaeI CATG 9 cut(s) 349, 418, 554, 565, 682, 1326, 1357, 1703, 1760
FalI AAGNNNNNCTT 4 cut(s) 1350, 1382, 1554, 1586
FatI CATG 9 cut(s) 345, 414, 550, 561, 678, 1322, 1353, 1699, 1756
FblI GTMKAC 2 cut(s) 988, 1766
Fnu4HI GCNGC 3 cut(s) 537, 847, 1209
FokI GGATG 1 cut(s) 1362
FriOI GRGCYC 3 cut(s) 85, 363, 1508
Fsp4HI GCNGC 3 cut(s) 537, 847, 1209
FspBI CTAG 6 cut(s) 240, 1148, 1160, 1250, 1538, 1608
GlaI GCGC 1 cut(s) 108
GluI GCNGC 3 cut(s) 537, 847, 1209
GsaI CCCAGC 1 cut(s) 977
GsuI CTGGAG 2 cut(s) 393, 1502
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 2 cut(s) 317, 815
HapII CCGG 1 cut(s) 449
HhaI GCGC 1 cut(s) 109
Hin1II CATG 9 cut(s) 349, 418, 554, 565, 682, 1326, 1357, 1703, 1760
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 2 cut(s) 777, 1543
HindII GTYRAC 2 cut(s) 777, 1543
HinfI GANTC 3 cut(s) 113, 341, 1241
HpaI GTTAAC 1 cut(s) 777
HpaII CCGG 1 cut(s) 449
HphI GGTGA 1 cut(s) 1043
Hpy166II GTNNAC 7 cut(s) 777, 800, 989, 1274, 1451, 1543, 1767
Hpy188III TCNNGA 6 cut(s) 449, 668, 1061, 1379, 1456, 1574
Hpy8I GTNNAC 7 cut(s) 777, 800, 989, 1274, 1451, 1543, 1767
Hpy99I CGWCG 2 cut(s) 307, 1697
HpyAV CCTTC 7 cut(s) 72, 201, 449, 965, 1299, 1315, 1620
HpyCH4III ACNGT 6 cut(s) 232, 781, 1278, 1599, 1764, 1771
HpyCH4IV ACGT 1 cut(s) 802
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 1448, 1753
HpyF3I CTNAG 7 cut(s) 484, 747, 842, 1093, 1622, 1752, 1776
HpySE526I ACGT 1 cut(s) 802
Hsp92II CATG 9 cut(s) 349, 418, 554, 565, 682, 1326, 1357, 1703, 1760
HspAI GCGC 1 cut(s) 107
Kpn2I TCCGGA 1 cut(s) 448
KpnI GGTACC 1 cut(s) 1054
KspAI GTTAAC 1 cut(s) 777
Kzo9I GATC 6 cut(s) 235, 249, 529, 724, 1567, 1629
LmnI GCTCC 2 cut(s) 202, 451
Lsp1109I GCAGC 2 cut(s) 523, 1195
LweI GCATC 2 cut(s) 733, 1543
MaeI CTAG 6 cut(s) 240, 1148, 1160, 1250, 1538, 1608
MaeII ACGT 1 cut(s) 802
MaeIII GTNAC 4 cut(s) 665, 1154, 1201, 1360
MalI GATC 6 cut(s) 237, 251, 531, 726, 1569, 1631
MboI GATC 6 cut(s) 235, 249, 529, 724, 1567, 1629
MboII GAAGA 7 cut(s) 19, 22, 518, 779, 1416, 1562, 1574
MfeI CAATTG 1 cut(s) 728
MflI RGATCY 2 cut(s) 235, 529
MhlI GDGCHC 4 cut(s) 85, 363, 1453, 1508
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 7 cut(s) 138, 307, 445, 490, 1082, 1177, 1261
MroI TCCGGA 1 cut(s) 448
MslI CAYNNNNRTG 4 cut(s) 441, 795, 1358, 1704
MspA1I CMGCKG 1 cut(s) 1208
MspI CCGG 1 cut(s) 449
MspR9I CCNGG 1 cut(s) 372
MunI CAATTG 1 cut(s) 728
Mva1269I GAATGC 2 cut(s) 849, 1747
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 3 cut(s) 104, 1448, 1753
NcoI CCATGG 1 cut(s) 345
NdeII GATC 6 cut(s) 235, 249, 529, 724, 1567, 1629
NheI GCTAGC 1 cut(s) 1147
NlaIII CATG 9 cut(s) 349, 418, 554, 565, 682, 1326, 1357, 1703, 1760
NlaIV GGNNCC 1 cut(s) 1052
NmuCI GTSAC 3 cut(s) 665, 1154, 1360
NspI RCATGY 1 cut(s) 1703
NspV TTCGAA 1 cut(s) 1011
PctI GAATGC 2 cut(s) 849, 1747
PfeI GAWTC 2 cut(s) 341, 1241
PflFI GACNNNGTC 1 cut(s) 1160
PkrI GCNGC 3 cut(s) 538, 848, 1210
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
PshAI GACNNNNGTC 1 cut(s) 1737
PshBI ATTAAT 2 cut(s) 707, 1025
Psp6I CCWGG 1 cut(s) 370
PspFI CCCAGC 1 cut(s) 973
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 1052
PspPI GGNCC 3 cut(s) 136, 316, 813
PstI CTGCAG 1 cut(s) 770
PsuI RGATCY 2 cut(s) 235, 529
PsyI GACNNNGTC 1 cut(s) 1160
PvuII CAGCTG 1 cut(s) 1208
RsaI GTAC 3 cut(s) 579, 1052, 1783
RsaNI GTAC 3 cut(s) 578, 1051, 1782
RseI CAYNNNNRTG 4 cut(s) 441, 795, 1358, 1704
SatI GCNGC 3 cut(s) 537, 847, 1209
Sau3AI GATC 6 cut(s) 235, 249, 529, 724, 1567, 1629
Sau96I GGNCC 3 cut(s) 136, 316, 813
SbfI CCTGCAGG 1 cut(s) 770
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 372
SdaI CCTGCAGG 1 cut(s) 770
SduI GDGCHC 4 cut(s) 85, 363, 1453, 1508
SfaNI GCATC 2 cut(s) 733, 1543
SfcI CTRYAG 5 cut(s) 131, 766, 881, 1614, 1767
SfuI TTCGAA 1 cut(s) 1011
SinI GGWCC 1 cut(s) 136
SmiI ATTTAAAT 2 cut(s) 15, 474
SmiMI CAYNNNNRTG 4 cut(s) 441, 795, 1358, 1704
Sse8387I CCTGCAGG 1 cut(s) 770
SsiI CCGC 4 cut(s) 98, 180, 847, 1265
SspMI CTAG 6 cut(s) 240, 1148, 1160, 1250, 1538, 1608
StyD4I CCNGG 1 cut(s) 370
StyI CCWWGG 4 cut(s) 345, 640, 816, 1674
SwaI ATTTAAAT 2 cut(s) 15, 474
TaaI ACNGT 6 cut(s) 232, 781, 1278, 1599, 1764, 1771
TaiI ACGT 1 cut(s) 805
TaqI TCGA 2 cut(s) 1011, 1483
TatI WGTACW 1 cut(s) 577
TauI GCSGC 1 cut(s) 849
TfiI GAWTC 2 cut(s) 341, 1241
TscAI CASTG 3 cut(s) 390, 492, 755
TseFI GTSAC 3 cut(s) 665, 1154, 1360
TseI GCWGC 2 cut(s) 536, 1208
Tsp45I GTSAC 3 cut(s) 665, 1154, 1360
TspDTI ATGAA 7 cut(s) 17, 425, 695, 779, 983, 1254, 1773
TspGWI ACGGA 3 cut(s) 277, 644, 1064
TspRI CASTG 3 cut(s) 390, 492, 755
Tth111I GACNNNGTC 1 cut(s) 1160
VneI GTGCAC 1 cut(s) 1449
VpaK11BI GGWCC 1 cut(s) 136
VspI ATTAAT 2 cut(s) 707, 1025
XapI RAATTY 2 cut(s) 41, 1192
XceI RCATGY 1 cut(s) 1703
XmiI GTMKAC 2 cut(s) 988, 1766
XspI CTAG 6 cut(s) 240, 1148, 1160, 1250, 1538, 1608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.